AlfalfaGEDB | MsG0480023246.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023246.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023246.01.T01	KEH20775.1	91.803	61	5	0	1	61	16	76	4.01E-33	120

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005184.01	MsG0480023246.01	0.811680	6.202502e-51	3.863698e-48
MsG0180005339.01	MsG0480023246.01	0.800226	1.592116e-48	7.373447e-46
MsG0280010728.01	MsG0480023246.01	0.833695	4.599305e-56	5.337190e-53
MsG0380014467.01	MsG0480023246.01	0.827102	1.883003e-54	1.800058e-51
MsG0480018848.01	MsG0480023246.01	0.816763	4.676587e-52	3.343375e-49
MsG0480019284.01	MsG0480023246.01	0.824097	9.706697e-54	8.514452e-51
MsG0480019285.01	MsG0480023246.01	0.809546	1.793716e-50	1.056182e-47
MsG0480023246.01	MsG0580027803.01	0.828874	7.052411e-55	7.098526e-52
MsG0480023246.01	MsG0580027899.01	0.823844	1.112821e-53	9.692878e-51
MsG0480023246.01	MsG0580029312.01	0.809993	1.437563e-50	8.565369e-48
MsG0480023246.01	MsG0680035665.01	0.803982	2.688493e-49	1.370103e-46
MsG0480023246.01	MsG0880047614.01	0.850512	1.618524e-60	3.177783e-57
MsG0280008072.01	MsG0480023246.01	0.828583	8.294509e-55	8.279122e-52
MsG0280008139.01	MsG0480023246.01	0.811007	8.680713e-51	5.313363e-48
MsG0280008223.01	MsG0480023246.01	0.806418	8.306328e-50	4.506055e-47
MsG0280008419.01	MsG0480023246.01	0.832087	1.154779e-55	1.277349e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023246.01.T01	MTR_8g089830	91.803	61	5	0	1	61	16	76	4.86e-37	120
MsG0480023246.01.T01	MTR_8g089825	65.152	66	19	3	1	64	16	79	1.20e-20	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTGATCTTCCTCAACTAT+TGG	0.360870	4:+82612554	None:intergenic
AGATCAATCCTACTATTTAG+AGG	0.394992	4:-82612540	MsG0480023246.01.T01:CDS
TTACGAACCCGCATACTATC+CGG	0.409986	4:-82612618	MsG0480023246.01.T01:CDS
TACGAACCCGCATACTATCC+GGG	0.436849	4:-82612617	MsG0480023246.01.T01:CDS
ACGACGAACCTCTAAATAGT+AGG	0.482372	4:+82612532	None:intergenic
AGACCGATCGACGAGGTTTG+AGG	0.483942	4:-82612496	MsG0480023246.01.T01:CDS
GATCGACGAGGTTTGAGGTA+TGG	0.517220	4:-82612491	MsG0480023246.01.T01:CDS
GCGTCGTGTTGCAATATATG+AGG	0.518107	4:-82612579	MsG0480023246.01.T01:CDS
ATACCTCAAACCTCGTCGAT+CGG	0.542925	4:+82612493	None:intergenic
TTATTCAACACGAGCATAGG+AGG	0.567055	4:-82612416	MsG0480023246.01.T01:CDS
GTGATGCCCCGGATAGTATG+CGG	0.591042	4:+82612610	None:intergenic
TGATGCCCCGGATAGTATGC+GGG	0.606955	4:+82612611	None:intergenic
ATATGAGGAACCAATAGTTG+AGG	0.635820	4:-82612564	MsG0480023246.01.T01:CDS
TCAACACGAGCATAGGAGGA+CGG	0.662040	4:-82612412	MsG0480023246.01.T01:CDS
ACGAACCCGCATACTATCCG+GGG	0.673282	4:-82612616	MsG0480023246.01.T01:CDS
GAGACTGAGACCGATCGACG+AGG	0.700755	4:-82612503	MsG0480023246.01.T01:CDS
AAGTGTTGATGAAGAAGCTG+AGG	0.703686	4:-82612442	MsG0480023246.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGATCAATCCTACTATTTAG+AGG	-	Chr4:82612495-82612514	MsG0480023246.01.T01:CDS	30.0%
	ATATGAGGAACCAATAGTTG+AGG	-	Chr4:82612471-82612490	MsG0480023246.01.T01:CDS	35.0%
	GATTGATCTTCCTCAACTAT+TGG	+	Chr4:82612484-82612503	None:intergenic	35.0%
!	CTTTTATTCAACACGAGCAT+AGG	-	Chr4:82612616-82612635	MsG0480023246.01.T01:CDS	35.0%
	ACGACGAACCTCTAAATAGT+AGG	+	Chr4:82612506-82612525	None:intergenic	40.0%
	TTATTCAACACGAGCATAGG+AGG	-	Chr4:82612619-82612638	MsG0480023246.01.T01:CDS	40.0%
!!	AAGTGTTGATGAAGAAGCTG+AGG	-	Chr4:82612593-82612612	MsG0480023246.01.T01:CDS	40.0%
	ATACCTCAAACCTCGTCGAT+CGG	+	Chr4:82612545-82612564	None:intergenic	45.0%
	GCGTCGTGTTGCAATATATG+AGG	-	Chr4:82612456-82612475	MsG0480023246.01.T01:CDS	45.0%
	TTACGAACCCGCATACTATC+CGG	-	Chr4:82612417-82612436	MsG0480023246.01.T01:CDS	45.0%
!	TTGCAACACGACGCTTTTGA+TGG	+	Chr4:82612450-82612469	None:intergenic	45.0%
	TACGAACCCGCATACTATCC+GGG	-	Chr4:82612418-82612437	MsG0480023246.01.T01:CDS	50.0%
	TCAACACGAGCATAGGAGGA+CGG	-	Chr4:82612623-82612642	MsG0480023246.01.T01:CDS	50.0%
!	GATCGACGAGGTTTGAGGTA+TGG	-	Chr4:82612544-82612563	MsG0480023246.01.T01:CDS	50.0%
	ACGAACCCGCATACTATCCG+GGG	-	Chr4:82612419-82612438	MsG0480023246.01.T01:CDS	55.0%
	AGACCGATCGACGAGGTTTG+AGG	-	Chr4:82612539-82612558	MsG0480023246.01.T01:CDS	55.0%
	GTGATGCCCCGGATAGTATG+CGG	+	Chr4:82612428-82612447	None:intergenic	55.0%
	TGATGCCCCGGATAGTATGC+GGG	+	Chr4:82612427-82612446	None:intergenic	55.0%
!!!	CGCTTTTGATGGTGATGCCC+CGG	+	Chr4:82612439-82612458	None:intergenic	55.0%
	GAGACTGAGACCGATCGACG+AGG	-	Chr4:82612532-82612551	MsG0480023246.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	82612406	82612651	82612406	ID=MsG0480023246.01;Name=MsG0480023246.01
Chr4	mRNA	82612406	82612651	82612406	ID=MsG0480023246.01.T01;Parent=MsG0480023246.01;Name=MsG0480023246.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|81
Chr4	exon	82612406	82612651	82612406	ID=MsG0480023246.01.T01:exon:944;Parent=MsG0480023246.01.T01
Chr4	CDS	82612406	82612651	82612406	ID=MsG0480023246.01.T01:cds;Parent=MsG0480023246.01.T01

Gene Sequence	>MsG0480023246.01.T01 ATGCACGAAATTTACGAACCCGCATACTATCCGGGGCATCACCATCAAAAGCGTCGTGTTGCAATATATGAGGAACCAATAGTTGAGGAAGATCAATCCTACTATTTAGAGGTTCGTCGTGAGACTGAGACTGAGACCGATCGACGAGGTTTGAGGTATGGAGATCAAAATGTTAGCACTACTATGAAAGTGTTGATGAAGAAGCTGAGGCTTTTATTCAACACGAGCATAGGAGGACGGCATTAG
Protein sequence	>MsG0480023246.01.T01 MHEIYEPAYYPGHHHQKRRVAIYEEPIVEEDQSYYLEVRRETETETDRRGLRYGDQNVSTTMKVLMKKLRLLFNTSIGGRH*