Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015231.01.T01 | AES70901.1 | 97.778 | 90 | 2 | 0 | 13 | 102 | 123 | 212 | 1.03E-54 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015231.01.T01 | Q96324 | 52.222 | 90 | 43 | 0 | 13 | 102 | 123 | 212 | 1.77E-30 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015231.01.T01 | A0A2Z2Z7E2 | 97.778 | 90 | 2 | 0 | 13 | 102 | 123 | 212 | 4.93e-55 | 179 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000192.01 | MsG0380015231.01 | 0.854924 | 8.901651e-62 | 2.020240e-58 |
| MsG0180004149.01 | MsG0380015231.01 | 0.804884 | 1.743618e-49 | 9.095179e-47 |
| MsG0380014706.01 | MsG0380015231.01 | 0.817908 | 2.582825e-52 | 1.905309e-49 |
| MsG0380015192.01 | MsG0380015231.01 | 0.895427 | 9.688843e-76 | 1.005011e-71 |
| MsG0380015231.01 | MsG0580024443.01 | 0.845420 | 4.106376e-59 | 6.839240e-56 |
| MsG0380015231.01 | MsG0580026511.01 | 0.805980 | 1.026994e-49 | 5.509761e-47 |
| MsG0380015231.01 | MsG0580028960.01 | 0.808387 | 3.175417e-50 | 1.813279e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015231.01.T01 | MTR_3g064700 | 97.778 | 90 | 2 | 0 | 13 | 102 | 123 | 212 | 1.25e-58 | 179 |
| MsG0380015231.01.T01 | MTR_3g450790 | 56.322 | 87 | 38 | 0 | 15 | 101 | 124 | 210 | 1.28e-28 | 103 |
| MsG0380015231.01.T01 | MTR_3g450930 | 54.023 | 87 | 40 | 0 | 15 | 101 | 124 | 210 | 2.86e-28 | 102 |
| MsG0380015231.01.T01 | MTR_5g090920 | 52.874 | 87 | 40 | 1 | 14 | 100 | 125 | 210 | 3.64e-26 | 96.7 |
| MsG0380015231.01.T01 | MTR_5g090910 | 46.237 | 93 | 50 | 0 | 9 | 101 | 62 | 154 | 1.01e-25 | 94.0 |
| MsG0380015231.01.T01 | MTR_5g090910 | 46.237 | 93 | 50 | 0 | 9 | 101 | 119 | 211 | 3.74e-25 | 94.4 |
| MsG0380015231.01.T01 | MTR_1g026140 | 52.500 | 80 | 34 | 1 | 26 | 101 | 139 | 218 | 1.23e-20 | 82.4 |
| MsG0380015231.01.T01 | MTR_1g088845 | 47.126 | 87 | 45 | 1 | 12 | 98 | 123 | 208 | 1.49e-20 | 82.0 |
| MsG0380015231.01.T01 | MTR_1g088840 | 44.828 | 87 | 47 | 1 | 12 | 98 | 27 | 112 | 1.96e-20 | 79.3 |
| MsG0380015231.01.T01 | MTR_1g088840 | 44.828 | 87 | 47 | 1 | 12 | 98 | 76 | 161 | 4.11e-20 | 80.1 |
| MsG0380015231.01.T01 | MTR_1g088840 | 44.828 | 87 | 47 | 1 | 12 | 98 | 123 | 208 | 8.36e-20 | 80.1 |
| MsG0380015231.01.T01 | MTR_1g088825 | 44.828 | 87 | 47 | 1 | 12 | 98 | 123 | 208 | 8.36e-20 | 80.1 |
| MsG0380015231.01.T01 | MTR_1g088850 | 44.048 | 84 | 46 | 1 | 15 | 98 | 126 | 208 | 7.19e-18 | 75.5 |
| MsG0380015231.01.T01 | MTR_1g492670 | 46.032 | 63 | 33 | 1 | 36 | 98 | 2 | 63 | 9.37e-14 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015231.01.T01 | AT3G03190 | 52.222 | 90 | 43 | 0 | 13 | 102 | 123 | 212 | 1.80e-31 | 110 |
| MsG0380015231.01.T01 | AT2G30870 | 57.143 | 84 | 36 | 0 | 15 | 98 | 124 | 207 | 2.09e-27 | 100 |
| MsG0380015231.01.T01 | AT2G30860 | 47.423 | 97 | 51 | 0 | 1 | 97 | 110 | 206 | 1.66e-25 | 95.1 |
| MsG0380015231.01.T01 | AT5G17220 | 42.045 | 88 | 51 | 0 | 15 | 102 | 125 | 212 | 8.24e-23 | 88.2 |
| MsG0380015231.01.T01 | AT2G47730 | 49.425 | 87 | 43 | 1 | 15 | 101 | 175 | 260 | 6.09e-20 | 81.6 |
| MsG0380015231.01.T01 | AT2G47730 | 49.425 | 87 | 43 | 1 | 15 | 101 | 175 | 260 | 6.09e-20 | 81.6 |
| MsG0380015231.01.T01 | AT3G62760 | 45.238 | 84 | 45 | 1 | 15 | 98 | 126 | 208 | 1.50e-19 | 79.7 |
| MsG0380015231.01.T01 | AT1G49860 | 48.235 | 85 | 42 | 1 | 15 | 97 | 128 | 212 | 8.08e-19 | 78.6 |
| MsG0380015231.01.T01 | AT1G02940 | 42.169 | 83 | 47 | 1 | 15 | 97 | 162 | 243 | 3.95e-18 | 76.6 |
| MsG0380015231.01.T01 | AT1G02940 | 42.169 | 83 | 47 | 1 | 15 | 97 | 162 | 243 | 3.95e-18 | 76.6 |
| MsG0380015231.01.T01 | AT1G02940 | 42.169 | 83 | 47 | 1 | 15 | 97 | 175 | 256 | 4.54e-18 | 76.6 |
| MsG0380015231.01.T01 | AT1G02940 | 42.169 | 83 | 47 | 1 | 15 | 97 | 187 | 268 | 5.29e-18 | 76.6 |
| MsG0380015231.01.T01 | AT1G02920 | 47.619 | 84 | 43 | 1 | 15 | 98 | 126 | 208 | 9.95e-18 | 74.7 |
| MsG0380015231.01.T01 | AT4G02520 | 43.529 | 85 | 47 | 1 | 15 | 99 | 129 | 212 | 7.34e-17 | 72.4 |
| MsG0380015231.01.T01 | AT1G02930 | 45.238 | 84 | 45 | 1 | 15 | 98 | 125 | 207 | 1.71e-16 | 71.6 |
| MsG0380015231.01.T01 | AT1G02930 | 45.238 | 84 | 45 | 1 | 15 | 98 | 125 | 207 | 1.71e-16 | 71.6 |
| MsG0380015231.01.T01 | AT1G02950 | 43.902 | 82 | 45 | 1 | 15 | 96 | 122 | 202 | 3.59e-16 | 70.9 |
| MsG0380015231.01.T01 | AT2G02930 | 45.000 | 80 | 43 | 1 | 19 | 98 | 133 | 211 | 4.71e-16 | 70.5 |
| MsG0380015231.01.T01 | AT1G02950 | 43.902 | 82 | 45 | 1 | 15 | 96 | 160 | 240 | 8.22e-16 | 70.5 |
| MsG0380015231.01.T01 | AT1G02950 | 43.902 | 82 | 45 | 1 | 15 | 96 | 148 | 228 | 8.57e-16 | 70.1 |
| MsG0380015231.01.T01 | AT1G02950 | 43.902 | 82 | 45 | 1 | 15 | 96 | 148 | 228 | 8.57e-16 | 70.1 |
| MsG0380015231.01.T01 | AT1G02950 | 43.902 | 82 | 45 | 1 | 15 | 96 | 150 | 230 | 9.55e-16 | 70.1 |
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTATTGAGGCAGCAAAAT+TGG | 0.165753 | 3:+67128457 | MsG0380015231.01.T01:CDS |
| GCTGATCTTAGCCATCTTCC+TGG | 0.323812 | 3:+67128425 | MsG0380015231.01.T01:CDS |
| TCTAAAAGCACTTATCTTGC+TGG | 0.332817 | 3:+67128386 | MsG0380015231.01.T01:CDS |
| TTGGAAGAAGTTGAAGGAAT+TGG | 0.377135 | 3:+67128544 | MsG0380015231.01.T01:CDS |
| TCAATAAGATGTTCAATTCC+AGG | 0.412097 | 3:-67128443 | None:intergenic |
| CTTATTGAGGCAGCAAAATT+GGG | 0.415280 | 3:+67128458 | MsG0380015231.01.T01:CDS |
| TCCTTCAACTTCTTCCAAGC+TGG | 0.444171 | 3:-67128539 | None:intergenic |
| AAATTTCTAGTAGGCCAGCT+TGG | 0.510403 | 3:+67128525 | MsG0380015231.01.T01:CDS |
| TGCTGGTGATAAGTTCACTT+TGG | 0.510705 | 3:+67128403 | MsG0380015231.01.T01:CDS |
| GGTGGGAGAAAATTTCTAGT+AGG | 0.512715 | 3:+67128516 | MsG0380015231.01.T01:CDS |
| AAGTTGAAGGAATTGGCATA+AGG | 0.515297 | 3:+67128551 | MsG0380015231.01.T01:CDS |
| GATGTTCAATTCCAGGAAGA+TGG | 0.515702 | 3:-67128436 | None:intergenic |
| TGGAATTGAACATCTTATTG+AGG | 0.518417 | 3:+67128445 | MsG0380015231.01.T01:CDS |
| TGAGCTATAGTTATGTCTCC+AGG | 0.558174 | 3:-67128311 | None:intergenic |
| GCCAGCTTGGAAGAAGTTGA+AGG | 0.576600 | 3:+67128538 | MsG0380015231.01.T01:CDS |
| AGAGGAAGAATGTGAATGCT+TGG | 0.577760 | 3:+67128495 | MsG0380015231.01.T01:CDS |
| GAAGAATGTGAATGCTTGGT+GGG | 0.598085 | 3:+67128499 | MsG0380015231.01.T01:CDS |
| GGAAGAATGTGAATGCTTGG+TGG | 0.600496 | 3:+67128498 | MsG0380015231.01.T01:CDS |
| TGGGACATTTGATTAGTGAG+AGG | 0.608261 | 3:+67128477 | MsG0380015231.01.T01:CDS |
| TGCTTGATGTGTATGAACGT+AGG | 0.749850 | 3:+67128360 | MsG0380015231.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTTGTAATTCTACCAAAAA+TGG | + | Chr3:67128284-67128303 | MsG0380015231.01.T01:CDS | 25.0% |
| TCAATAAGATGTTCAATTCC+AGG | - | Chr3:67128446-67128465 | None:intergenic | 30.0% | |
| TGGAATTGAACATCTTATTG+AGG | + | Chr3:67128445-67128464 | MsG0380015231.01.T01:CDS | 30.0% | |
| CTTATTGAGGCAGCAAAATT+GGG | + | Chr3:67128458-67128477 | MsG0380015231.01.T01:CDS | 35.0% | |
| TCTTATTGAGGCAGCAAAAT+TGG | + | Chr3:67128457-67128476 | MsG0380015231.01.T01:CDS | 35.0% | |
| TTGGAAGAAGTTGAAGGAAT+TGG | + | Chr3:67128544-67128563 | MsG0380015231.01.T01:CDS | 35.0% | |
| !! | TCTAAAAGCACTTATCTTGC+TGG | + | Chr3:67128386-67128405 | MsG0380015231.01.T01:CDS | 35.0% |
| AGAGGAAGAATGTGAATGCT+TGG | + | Chr3:67128495-67128514 | MsG0380015231.01.T01:CDS | 40.0% | |
| GAAGAATGTGAATGCTTGGT+GGG | + | Chr3:67128499-67128518 | MsG0380015231.01.T01:CDS | 40.0% | |
| GATGTTCAATTCCAGGAAGA+TGG | - | Chr3:67128439-67128458 | None:intergenic | 40.0% | |
| TCTACCAAAAATGGTAAGCC+TGG | + | Chr3:67128293-67128312 | MsG0380015231.01.T01:CDS | 40.0% | |
| TGAGCTATAGTTATGTCTCC+AGG | - | Chr3:67128314-67128333 | None:intergenic | 40.0% | |
| TGCTGGTGATAAGTTCACTT+TGG | + | Chr3:67128403-67128422 | MsG0380015231.01.T01:CDS | 40.0% | |
| TGCTTGATGTGTATGAACGT+AGG | + | Chr3:67128360-67128379 | MsG0380015231.01.T01:CDS | 40.0% | |
| TGGGACATTTGATTAGTGAG+AGG | + | Chr3:67128477-67128496 | MsG0380015231.01.T01:CDS | 40.0% | |
| !! | AAATTTCTAGTAGGCCAGCT+TGG | + | Chr3:67128525-67128544 | MsG0380015231.01.T01:CDS | 40.0% |
| !! | GGTGGGAGAAAATTTCTAGT+AGG | + | Chr3:67128516-67128535 | MsG0380015231.01.T01:CDS | 40.0% |
| GGAAGAATGTGAATGCTTGG+TGG | + | Chr3:67128498-67128517 | MsG0380015231.01.T01:CDS | 45.0% | |
| TCCTTCAACTTCTTCCAAGC+TGG | - | Chr3:67128542-67128561 | None:intergenic | 45.0% | |
| ! | GTCTCCAGGCTTACCATTTT+TGG | - | Chr3:67128300-67128319 | None:intergenic | 45.0% |
| GCCAGCTTGGAAGAAGTTGA+AGG | + | Chr3:67128538-67128557 | MsG0380015231.01.T01:CDS | 50.0% | |
| GCTGATCTTAGCCATCTTCC+TGG | + | Chr3:67128425-67128444 | MsG0380015231.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 67128263 | 67128571 | 67128263 | ID=MsG0380015231.01;Name=MsG0380015231.01 |
| Chr3 | mRNA | 67128263 | 67128571 | 67128263 | ID=MsG0380015231.01.T01;Parent=MsG0380015231.01;Name=MsG0380015231.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|102 |
| Chr3 | exon | 67128263 | 67128571 | 67128263 | ID=MsG0380015231.01.T01:exon:33;Parent=MsG0380015231.01.T01 |
| Chr3 | CDS | 67128263 | 67128571 | 67128263 | ID=MsG0380015231.01.T01:cds;Parent=MsG0380015231.01.T01 |
| Gene Sequence |
| Protein sequence |
