Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015192.01.T01 | XP_003600650.2 | 100 | 147 | 0 | 0 | 11 | 157 | 66 | 212 | 7.01E-103 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015192.01.T01 | Q96324 | 54.422 | 147 | 67 | 0 | 11 | 157 | 66 | 212 | 5.93E-55 | 175 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015192.01.T01 | A0A2Z2Z7E2 | 100.000 | 147 | 0 | 0 | 11 | 157 | 66 | 212 | 3.35e-103 | 303 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000192.01 | MsG0380015192.01 | 0.829571 | 4.779645e-55 | 4.910608e-52 |
| MsG0180004249.01 | MsG0380015192.01 | 0.804356 | 2.247031e-49 | 1.156143e-46 |
| MsG0280010712.01 | MsG0380015192.01 | 0.812049 | 5.155302e-51 | 3.243381e-48 |
| MsG0380015192.01 | MsG0380015231.01 | 0.895427 | 9.688843e-76 | 1.005011e-71 |
| MsG0380015192.01 | MsG0380015332.01 | 0.814468 | 1.517156e-51 | 1.018882e-48 |
| MsG0380015192.01 | MsG0480023488.01 | 0.805272 | 1.446066e-49 | 7.617732e-47 |
| MsG0380015192.01 | MsG0580024443.01 | 0.815184 | 1.052817e-51 | 7.207850e-49 |
| MsG0380015192.01 | MsG0580026511.01 | 0.830115 | 3.521142e-55 | 3.675360e-52 |
| MsG0380015192.01 | MsG0580029925.01 | 0.808127 | 3.608646e-50 | 2.046511e-47 |
| MsG0380015192.01 | MsG0780040074.01 | 0.805788 | 1.127147e-49 | 6.017254e-47 |
| MsG0380015192.01 | MsG0880046531.01 | 0.801911 | 7.199828e-49 | 3.479878e-46 |
| MsG0280007311.01 | MsG0380015192.01 | 0.811600 | 6.456069e-51 | 4.013282e-48 |
| MsG0280009739.01 | MsG0380015192.01 | 0.807198 | 5.682142e-50 | 3.145204e-47 |
| MsG0280010443.01 | MsG0380015192.01 | 0.803693 | 3.086223e-49 | 1.561093e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015192.01.T01 | MTR_3g064700 | 99.320 | 147 | 1 | 0 | 11 | 157 | 66 | 212 | 3.65e-106 | 302 |
| MsG0380015192.01.T01 | MTR_3g450930 | 55.479 | 146 | 65 | 0 | 11 | 156 | 65 | 210 | 1.01e-56 | 177 |
| MsG0380015192.01.T01 | MTR_3g450790 | 55.479 | 146 | 65 | 0 | 11 | 156 | 65 | 210 | 7.88e-55 | 172 |
| MsG0380015192.01.T01 | MTR_5g090910 | 50.323 | 155 | 74 | 2 | 4 | 156 | 1 | 154 | 2.07e-49 | 156 |
| MsG0380015192.01.T01 | MTR_5g090920 | 53.378 | 148 | 64 | 3 | 11 | 155 | 65 | 210 | 5.49e-49 | 157 |
| MsG0380015192.01.T01 | MTR_5g090910 | 50.676 | 148 | 70 | 2 | 11 | 156 | 65 | 211 | 4.36e-46 | 150 |
| MsG0380015192.01.T01 | MTR_1g026140 | 45.033 | 151 | 78 | 2 | 11 | 156 | 68 | 218 | 1.70e-35 | 123 |
| MsG0380015192.01.T01 | MTR_1g088840 | 41.259 | 143 | 83 | 1 | 11 | 153 | 20 | 161 | 1.76e-33 | 116 |
| MsG0380015192.01.T01 | MTR_1g088840 | 41.259 | 143 | 83 | 1 | 11 | 153 | 67 | 208 | 5.52e-33 | 116 |
| MsG0380015192.01.T01 | MTR_1g088825 | 41.259 | 143 | 83 | 1 | 11 | 153 | 67 | 208 | 5.52e-33 | 116 |
| MsG0380015192.01.T01 | MTR_1g088850 | 41.667 | 144 | 82 | 2 | 11 | 153 | 66 | 208 | 8.68e-33 | 116 |
| MsG0380015192.01.T01 | MTR_1g088845 | 41.259 | 143 | 83 | 1 | 11 | 153 | 67 | 208 | 2.18e-31 | 112 |
| MsG0380015192.01.T01 | MTR_1g088840 | 42.727 | 110 | 62 | 1 | 44 | 153 | 4 | 112 | 1.19e-25 | 94.7 |
| MsG0380015192.01.T01 | MTR_1g492670 | 46.032 | 63 | 33 | 1 | 91 | 153 | 2 | 63 | 1.12e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015192.01.T01 | AT3G03190 | 54.422 | 147 | 67 | 0 | 11 | 157 | 66 | 212 | 6.05e-56 | 175 |
| MsG0380015192.01.T01 | AT2G30870 | 53.846 | 143 | 66 | 0 | 11 | 153 | 65 | 207 | 3.01e-49 | 158 |
| MsG0380015192.01.T01 | AT5G17220 | 46.939 | 147 | 78 | 0 | 11 | 157 | 66 | 212 | 1.01e-48 | 156 |
| MsG0380015192.01.T01 | AT2G30860 | 50.000 | 142 | 71 | 0 | 11 | 152 | 65 | 206 | 6.08e-45 | 147 |
| MsG0380015192.01.T01 | AT3G62760 | 43.056 | 144 | 80 | 2 | 11 | 153 | 66 | 208 | 8.65e-34 | 118 |
| MsG0380015192.01.T01 | AT1G49860 | 41.667 | 144 | 82 | 1 | 11 | 152 | 69 | 212 | 6.31e-32 | 114 |
| MsG0380015192.01.T01 | AT2G47730 | 41.722 | 151 | 86 | 2 | 7 | 156 | 111 | 260 | 6.19e-31 | 112 |
| MsG0380015192.01.T01 | AT2G47730 | 41.722 | 151 | 86 | 2 | 7 | 156 | 111 | 260 | 6.19e-31 | 112 |
| MsG0380015192.01.T01 | AT1G02950 | 41.497 | 147 | 84 | 2 | 6 | 151 | 83 | 228 | 3.05e-29 | 107 |
| MsG0380015192.01.T01 | AT1G02950 | 41.497 | 147 | 84 | 2 | 6 | 151 | 83 | 228 | 3.05e-29 | 107 |
| MsG0380015192.01.T01 | AT1G02950 | 41.259 | 143 | 82 | 2 | 10 | 151 | 61 | 202 | 3.13e-29 | 107 |
| MsG0380015192.01.T01 | AT1G02950 | 41.497 | 147 | 84 | 2 | 6 | 151 | 85 | 230 | 3.40e-29 | 107 |
| MsG0380015192.01.T01 | AT1G02950 | 41.497 | 147 | 84 | 2 | 6 | 151 | 95 | 240 | 3.68e-29 | 107 |
| MsG0380015192.01.T01 | AT1G02930 | 40.559 | 143 | 83 | 2 | 11 | 153 | 67 | 207 | 5.29e-29 | 106 |
| MsG0380015192.01.T01 | AT1G02930 | 40.559 | 143 | 83 | 2 | 11 | 153 | 67 | 207 | 5.29e-29 | 106 |
| MsG0380015192.01.T01 | AT1G02920 | 41.259 | 143 | 83 | 1 | 11 | 153 | 67 | 208 | 6.72e-29 | 105 |
| MsG0380015192.01.T01 | AT4G02520 | 38.095 | 147 | 87 | 2 | 11 | 154 | 67 | 212 | 2.92e-27 | 101 |
| MsG0380015192.01.T01 | AT1G02940 | 36.806 | 144 | 89 | 2 | 10 | 152 | 101 | 243 | 1.74e-26 | 100 |
| MsG0380015192.01.T01 | AT1G02940 | 36.806 | 144 | 89 | 2 | 10 | 152 | 101 | 243 | 1.74e-26 | 100 |
| MsG0380015192.01.T01 | AT1G02940 | 36.806 | 144 | 89 | 2 | 10 | 152 | 126 | 268 | 2.06e-26 | 101 |
| MsG0380015192.01.T01 | AT1G02940 | 36.806 | 144 | 89 | 2 | 10 | 152 | 114 | 256 | 2.20e-26 | 100 |
| MsG0380015192.01.T01 | AT2G02930 | 38.356 | 146 | 86 | 2 | 11 | 153 | 67 | 211 | 1.04e-25 | 97.8 |
| MsG0380015192.01.T01 | AT2G30860 | 47.778 | 90 | 47 | 0 | 11 | 100 | 65 | 154 | 3.08e-22 | 87.4 |
Find 31 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTATTGAGGCAGCAAAAT+TGG | 0.186477 | 3:-66523298 | MsG0380015192.01.T01:CDS |
| CTTAGTCATCTTCCTGGAAT+TGG | 0.292656 | 3:-66523324 | MsG0380015192.01.T01:CDS |
| GCTGATCTTAGTCATCTTCC+TGG | 0.315615 | 3:-66523330 | MsG0380015192.01.T01:CDS |
| TCTAAAAGCACTTATCTTGC+TGG | 0.332817 | 3:-66523369 | MsG0380015192.01.T01:CDS |
| TTGGAAGAAGTTGAAGGAAT+TGG | 0.377135 | 3:-66523211 | MsG0380015192.01.T01:CDS |
| CTTATTGAGGCAGCAAAATT+GGG | 0.415280 | 3:-66523297 | MsG0380015192.01.T01:CDS |
| CTGTGATTTGTGTAGAATCA+AGG | 0.424395 | 3:-66523641 | MsG0380015192.01.T01:CDS |
| TCCTTCAACTTCTTCCAAGC+TGG | 0.444171 | 3:+66523216 | None:intergenic |
| TCAATAAGATGTCCAATTCC+AGG | 0.451011 | 3:+66523312 | None:intergenic |
| CTTCTAAAGTTGTGCCAAGT+AGG | 0.455796 | 3:+66523571 | None:intergenic |
| AAAGTTGTGCCAAGTAGGTC+AGG | 0.467376 | 3:+66523576 | None:intergenic |
| AGAAAGCTGTGGTGGATCAA+TGG | 0.498575 | 3:-66523548 | MsG0380015192.01.T01:CDS |
| AAATTTCTAGTAGGCCAGCT+TGG | 0.510403 | 3:-66523230 | MsG0380015192.01.T01:CDS |
| TGCTGGTGATAAGTTCACTT+TGG | 0.510705 | 3:-66523352 | MsG0380015192.01.T01:CDS |
| GGTGGGAGAAAATTTCTAGT+AGG | 0.512715 | 3:-66523239 | MsG0380015192.01.T01:CDS |
| TAGAATCAAGGGCTATTGTA+AGG | 0.513820 | 3:-66523629 | MsG0380015192.01.T01:CDS |
| AAGTTGAAGGAATTGGCATA+AGG | 0.515297 | 3:-66523204 | None:intergenic |
| GACCGTGGTCCTGACCTACT+TGG | 0.545257 | 3:-66523585 | MsG0380015192.01.T01:CDS |
| TGAGCTATAGTTATGTCTCC+AGG | 0.558174 | 3:+66523444 | None:intergenic |
| TGTGATTTGTGTAGAATCAA+GGG | 0.558216 | 3:-66523640 | MsG0380015192.01.T01:CDS |
| GCCAGCTTGGAAGAAGTTGA+AGG | 0.576600 | 3:-66523217 | MsG0380015192.01.T01:CDS |
| AGAGGAAGAATGTGAATGCT+TGG | 0.577760 | 3:-66523260 | MsG0380015192.01.T01:CDS |
| TTTAGAAGAGAAAGCTGTGG+TGG | 0.591968 | 3:-66523556 | MsG0380015192.01.T01:CDS |
| TGGAATTGGACATCTTATTG+AGG | 0.594707 | 3:-66523310 | MsG0380015192.01.T01:CDS |
| GAAGAATGTGAATGCTTGGT+GGG | 0.598085 | 3:-66523256 | MsG0380015192.01.T01:CDS |
| GGAAGAATGTGAATGCTTGG+TGG | 0.600496 | 3:-66523257 | MsG0380015192.01.T01:CDS |
| TGGGACATTTGATTAGTGAG+AGG | 0.608261 | 3:-66523278 | MsG0380015192.01.T01:CDS |
| AACTTTAGAAGAGAAAGCTG+TGG | 0.697603 | 3:-66523559 | MsG0380015192.01.T01:CDS |
| TGCCAAGTAGGTCAGGACCA+CGG | 0.707978 | 3:+66523583 | None:intergenic |
| GCAGCAAAGTATGCAGACCG+TGG | 0.728704 | 3:-66523600 | MsG0380015192.01.T01:CDS |
| TGCTTGATGTGTATGAACGT+AGG | 0.749850 | 3:-66523395 | MsG0380015192.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTGTAATTCTACCAAAAAT+GGG | - | Chr3:66523392-66523411 | MsG0380015192.01.T01:CDS | 25.0% |
| ! | TCTTGTAATTCTACCAAAAA+TGG | - | Chr3:66523391-66523410 | MsG0380015192.01.T01:CDS | 25.0% |
| ! | TGTGATTTGTGTAGAATCAA+GGG | - | Chr3:66523223-66523242 | MsG0380015192.01.T01:CDS | 30.0% |
| CTTATTGAGGCAGCAAAATT+GGG | - | Chr3:66523566-66523585 | MsG0380015192.01.T01:CDS | 35.0% | |
| TAGAATCAAGGGCTATTGTA+AGG | - | Chr3:66523234-66523253 | MsG0380015192.01.T01:CDS | 35.0% | |
| TCAATAAGATGTCCAATTCC+AGG | + | Chr3:66523554-66523573 | None:intergenic | 35.0% | |
| TCTTATTGAGGCAGCAAAAT+TGG | - | Chr3:66523565-66523584 | MsG0380015192.01.T01:CDS | 35.0% | |
| TGGAATTGGACATCTTATTG+AGG | - | Chr3:66523553-66523572 | MsG0380015192.01.T01:CDS | 35.0% | |
| TTGGAAGAAGTTGAAGGAAT+TGG | - | Chr3:66523652-66523671 | MsG0380015192.01.T01:CDS | 35.0% | |
| ! | AACTTTAGAAGAGAAAGCTG+TGG | - | Chr3:66523304-66523323 | MsG0380015192.01.T01:CDS | 35.0% |
| ! | CTGTGATTTGTGTAGAATCA+AGG | - | Chr3:66523222-66523241 | MsG0380015192.01.T01:CDS | 35.0% |
| !! | TCTAAAAGCACTTATCTTGC+TGG | - | Chr3:66523494-66523513 | MsG0380015192.01.T01:CDS | 35.0% |
| AGAGGAAGAATGTGAATGCT+TGG | - | Chr3:66523603-66523622 | MsG0380015192.01.T01:CDS | 40.0% | |
| CTTAGTCATCTTCCTGGAAT+TGG | - | Chr3:66523539-66523558 | MsG0380015192.01.T01:CDS | 40.0% | |
| GAAGAATGTGAATGCTTGGT+GGG | - | Chr3:66523607-66523626 | MsG0380015192.01.T01:CDS | 40.0% | |
| TGAGCTATAGTTATGTCTCC+AGG | + | Chr3:66523422-66523441 | None:intergenic | 40.0% | |
| TGCTGGTGATAAGTTCACTT+TGG | - | Chr3:66523511-66523530 | MsG0380015192.01.T01:CDS | 40.0% | |
| TGCTTGATGTGTATGAACGT+AGG | - | Chr3:66523468-66523487 | MsG0380015192.01.T01:CDS | 40.0% | |
| TGGGACATTTGATTAGTGAG+AGG | - | Chr3:66523585-66523604 | MsG0380015192.01.T01:CDS | 40.0% | |
| TTTAGAAGAGAAAGCTGTGG+TGG | - | Chr3:66523307-66523326 | MsG0380015192.01.T01:CDS | 40.0% | |
| !! | AAATTTCTAGTAGGCCAGCT+TGG | - | Chr3:66523633-66523652 | MsG0380015192.01.T01:CDS | 40.0% |
| !! | CTTCTAAAGTTGTGCCAAGT+AGG | + | Chr3:66523295-66523314 | None:intergenic | 40.0% |
| !! | GGTGGGAGAAAATTTCTAGT+AGG | - | Chr3:66523624-66523643 | MsG0380015192.01.T01:CDS | 40.0% |
| AAAGTTGTGCCAAGTAGGTC+AGG | + | Chr3:66523290-66523309 | None:intergenic | 45.0% | |
| CTACCAAAAATGGGTAAGCC+TGG | - | Chr3:66523401-66523420 | MsG0380015192.01.T01:CDS | 45.0% | |
| GCTGATCTTAGTCATCTTCC+TGG | - | Chr3:66523533-66523552 | MsG0380015192.01.T01:CDS | 45.0% | |
| GGAAGAATGTGAATGCTTGG+TGG | - | Chr3:66523606-66523625 | MsG0380015192.01.T01:CDS | 45.0% | |
| TCCTTCAACTTCTTCCAAGC+TGG | + | Chr3:66523650-66523669 | None:intergenic | 45.0% | |
| ! | TCTCCAGGCTTACCCATTTT+TGG | + | Chr3:66523407-66523426 | None:intergenic | 45.0% |
| !! | AGAAAGCTGTGGTGGATCAA+TGG | - | Chr3:66523315-66523334 | MsG0380015192.01.T01:CDS | 45.0% |
| GCCAGCTTGGAAGAAGTTGA+AGG | - | Chr3:66523646-66523665 | MsG0380015192.01.T01:CDS | 50.0% | |
| GCAGCAAAGTATGCAGACCG+TGG | - | Chr3:66523263-66523282 | MsG0380015192.01.T01:CDS | 55.0% | |
| TGCCAAGTAGGTCAGGACCA+CGG | + | Chr3:66523283-66523302 | None:intergenic | 55.0% | |
| GACCGTGGTCCTGACCTACT+TGG | - | Chr3:66523278-66523297 | MsG0380015192.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 66523206 | 66523679 | 66523206 | ID=MsG0380015192.01;Name=MsG0380015192.01 |
| Chr3 | mRNA | 66523206 | 66523679 | 66523206 | ID=MsG0380015192.01.T01;Parent=MsG0380015192.01;Name=MsG0380015192.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|157 |
| Chr3 | exon | 66523206 | 66523679 | 66523206 | ID=MsG0380015192.01.T01:exon:23048;Parent=MsG0380015192.01.T01 |
| Chr3 | CDS | 66523206 | 66523679 | 66523206 | ID=MsG0380015192.01.T01:cds;Parent=MsG0380015192.01.T01 |
| Gene Sequence |
| Protein sequence |
