AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016317.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016317.01.T01 MTR_3g087700 88.144 194 12 3 1 193 1 184 2.89e-124 349
MsG0380016317.01.T01 MTR_3g087700 85.714 154 11 3 1 153 1 144 3.86e-91 263
MsG0380016317.01.T01 MTR_3g073960 33.898 177 74 7 1 177 1 134 2.62e-21 86.3
MsG0380016317.01.T01 MTR_8g008840 53.571 56 25 1 127 181 32 87 2.70e-15 69.3
MsG0380016317.01.T01 MTR_4g099050 61.702 47 18 0 131 177 71 117 7.41e-15 69.3
MsG0380016317.01.T01 MTR_5g013770 50.704 71 29 2 131 196 83 152 1.83e-14 68.6
MsG0380016317.01.T01 MTR_8g022950 44.286 70 36 1 133 202 39 105 8.78e-14 65.5
MsG0380016317.01.T01 MTR_4g124930 54.902 51 22 1 127 177 172 221 1.28e-11 62.4
MsG0380016317.01.T01 MTR_2g436020 58.537 41 17 0 133 173 64 104 1.30e-11 60.5
MsG0380016317.01.T01 MTR_2g436250 58.537 41 17 0 133 173 64 104 1.30e-11 60.5
MsG0380016317.01.T01 MTR_4g124930 54.902 51 22 1 127 177 172 221 1.31e-11 62.0
MsG0380016317.01.T01 MTR_2g042970 40.741 81 45 2 101 179 152 231 2.22e-11 61.6
MsG0380016317.01.T01 MTR_4g120160 43.548 62 34 1 114 175 44 104 5.88e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016317.01.T01 AT1G78020 33.495 206 93 7 1 206 1 162 9.12e-24 92.8
MsG0380016317.01.T01 AT1G22160 35.196 179 61 8 1 179 1 124 1.19e-19 81.6
MsG0380016317.01.T01 AT4G39795 45.833 72 36 1 112 183 55 123 6.14e-17 74.3
MsG0380016317.01.T01 AT4G17670 62.000 50 19 0 128 177 71 120 1.35e-16 73.9
MsG0380016317.01.T01 AT5G65040 51.389 72 31 1 114 185 42 109 1.66e-16 72.8
MsG0380016317.01.T01 AT2G44670 62.000 50 18 1 131 179 14 63 6.20e-16 70.5
MsG0380016317.01.T01 AT5G47060 63.830 47 17 0 131 177 95 141 5.75e-15 70.1
MsG0380016317.01.T01 AT5G47060 63.830 47 17 0 131 177 95 141 5.75e-15 70.1
MsG0380016317.01.T01 AT1G53885 48.529 68 33 1 109 176 20 85 1.78e-12 62.4
MsG0380016317.01.T01 AT1G53903 48.529 68 33 1 109 176 20 85 1.78e-12 62.4
MsG0380016317.01.T01 AT1G74940 45.902 61 32 1 132 192 149 208 5.34e-11 60.1
MsG0380016317.01.T01 AT1G19200 49.091 55 27 1 126 180 143 196 5.38e-11 60.1
MsG0380016317.01.T01 AT5G49120 39.130 69 42 0 105 173 40 108 5.89e-11 58.9
MsG0380016317.01.T01 AT1G19200 49.091 55 27 1 126 180 156 209 6.44e-11 60.1

Find 50 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTTCTTCTCATAAT+TGG 0.156033 3:+81919134 None:intergenic
CTTCCTCATGATGGGATTCT+AGG 0.211376 3:+81919060 None:intergenic
AATAATATTGTTAGTGTTTG+TGG 0.326456 3:+81918848 None:intergenic
AGTTACAGAGGCCACAAGTT+TGG 0.349033 3:+81918760 None:intergenic
ATGATGGGATTCTAGGTTGT+TGG 0.366587 3:+81919067 None:intergenic
ACAAGCAGCAGTAGAAGCTT+TGG 0.372677 3:+81918125 None:intergenic
TCTAGGTTGTTGGTTTGATC+AGG 0.381479 3:+81919077 None:intergenic
ATTGTTAGTGTTTGTGGCTG+TGG 0.394454 3:+81918854 None:intergenic
TGTAACTGTCGTTTGGCTCC+TGG 0.404985 3:-81918744 MsG0380016317.01.T01:CDS
TACACGGTGGTCTTCTTTGT+TGG 0.433148 3:+81918170 None:intergenic
TGGCCTCTGTAACTGTCGTT+TGG 0.455985 3:-81918751 MsG0380016317.01.T01:CDS
CTTGTTCTTCTCATAATTGG+TGG 0.464977 3:+81919137 None:intergenic
CACTGATTCTTCCTCATGAT+GGG 0.470672 3:+81919052 None:intergenic
GATTAGAAGAACATGTTGCT+TGG 0.489313 3:-81919173 None:intergenic
GATGGCTGATGAACGTTTAG+TGG 0.489958 3:-81918907 MsG0380016317.01.T01:CDS
TCACTGATTCTTCCTCATGA+TGG 0.491322 3:+81919051 None:intergenic
AAGACAAAAGAAAAGAAAAG+TGG 0.499141 3:-81918206 MsG0380016317.01.T01:CDS
GTTCCCATCACAACCGTATG+TGG 0.501901 3:+81918957 None:intergenic
GAATCCACATACGGTTGTGA+TGG 0.509624 3:-81918961 MsG0380016317.01.T01:CDS
TGATCATCATGGTGTGGATG+TGG 0.510086 3:+81919005 None:intergenic
GTGGCTGTGGCTGTGTGTGA+AGG 0.511685 3:+81918867 None:intergenic
GTCGAGACATCTACATGTAT+AGG 0.522956 3:-81918722 MsG0380016317.01.T01:intron
ATCAGGTGGTTGCATGTCCA+CGG 0.524115 3:+81919094 None:intergenic
AAGAAAAGAAAAGTGGAAGA+TGG 0.529987 3:-81918199 MsG0380016317.01.T01:CDS
CTTCTCATAATTGGTGGTCG+AGG 0.540724 3:+81919143 None:intergenic
AGGTTGTTGGTTTGATCAGG+TGG 0.542107 3:+81919080 None:intergenic
TGGCTGTGGCTGTGTGTGAA+GGG 0.543537 3:+81918868 None:intergenic
TGCCATGTTCTTGATCATCA+TGG 0.545211 3:+81918994 None:intergenic
AGCAGTAGAAGCTTTGGCAG+TGG 0.548192 3:+81918131 None:intergenic
GCTTTGGCAGTGGCCGTGCA+CGG 0.562038 3:+81918141 None:intergenic
TCATGTGCACTCACACTCAA+AGG 0.576185 3:+81918810 None:intergenic
CCGTGTATCACCACCGTGCA+CGG 0.577796 3:-81918154 MsG0380016317.01.T01:CDS
AATCCACATACGGTTGTGAT+GGG 0.578470 3:-81918960 MsG0380016317.01.T01:CDS
CCTCACTTTCTCCAAACTTG+TGG 0.579241 3:-81918771 MsG0380016317.01.T01:CDS
CCGTGCACGGTGGTGATACA+CGG 0.602872 3:+81918154 None:intergenic
CACCATGATGATCAAGAACA+TGG 0.605152 3:-81918996 MsG0380016317.01.T01:CDS
TGGTGTGGATGTGGCTGCAG+AGG 0.618105 3:+81919014 None:intergenic
ATGGCTGATGAACGTTTAGT+GGG 0.621978 3:-81918906 MsG0380016317.01.T01:CDS
CCACAAGTTTGGAGAAAGTG+AGG 0.629285 3:+81918771 None:intergenic
TGTTCTTGATCATCATGGTG+TGG 0.629577 3:+81918999 None:intergenic
CAACATCAAGAATCCACATA+CGG 0.631823 3:-81918970 MsG0380016317.01.T01:CDS
CATGAGTGGTGGTTTGTCCG+TGG 0.637137 3:-81919111 MsG0380016317.01.T01:CDS
AGAAGAACAAGAAGCATGAG+TGG 0.643370 3:-81919125 MsG0380016317.01.T01:CDS
TACATGTAGATGTCTCGACC+AGG 0.645212 3:+81918726 None:intergenic
CGTTAAAAGTGAATTAACGA+TGG 0.660105 3:-81918925 MsG0380016317.01.T01:CDS
CAACCTAGAATCCCATCATG+AGG 0.662994 3:-81919063 MsG0380016317.01.T01:CDS
TTGGCAGTGGCCGTGCACGG+TGG 0.682224 3:+81918144 None:intergenic
GAGCCAAACGACAGTTACAG+AGG 0.685876 3:+81918748 None:intergenic
AGAACAAGAAGCATGAGTGG+TGG 0.691730 3:-81919122 MsG0380016317.01.T01:CDS
TGCACGGTGGTGATACACGG+TGG 0.754526 3:+81918157 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAATAATAGAAAAAAA+TGG + Chr3:81918591-81918610 None:intergenic 10.0%
!!! TTAATTAATTTTTTGTTTAG+AGG - Chr3:81918928-81918947 MsG0380016317.01.T01:CDS 10.0%
!! GTTAGTTAGTATAATTAATT+TGG - Chr3:81918959-81918978 MsG0380016317.01.T01:CDS 15.0%
!!! AAAGAAAATTATTGATTTCA+GGG - Chr3:81919003-81919022 MsG0380016317.01.T01:CDS 15.0%
!! AATAATATTGTTAGTGTTTG+TGG + Chr3:81918435-81918454 None:intergenic 20.0%
!! GTAAATATTAATTCTTCCTT+TGG + Chr3:81918818-81918837 None:intergenic 20.0%
!!! AAGAAAATTATTGATTTCAG+GGG - Chr3:81919004-81919023 MsG0380016317.01.T01:CDS 20.0%
!!! GAAAGAAAATTATTGATTTC+AGG - Chr3:81919002-81919021 MsG0380016317.01.T01:CDS 20.0%
! AAGACAAAAGAAAAGAAAAG+TGG - Chr3:81919074-81919093 MsG0380016317.01.T01:CDS 25.0%
! CTGAAAATAAAGAGACAATT+AGG + Chr3:81918788-81918807 None:intergenic 25.0%
! TAAACAAGAATATCAAAGTC+AGG - Chr3:81918698-81918717 MsG0380016317.01.T01:intron 25.0%
!! TGACTTTGATATTCTTGTTT+AGG + Chr3:81918699-81918718 None:intergenic 25.0%
!!! TTTTTGTTTAGAGGAAATTG+TGG - Chr3:81918937-81918956 MsG0380016317.01.T01:CDS 25.0%
AAGAAAAGAAAAGTGGAAGA+TGG - Chr3:81919081-81919100 MsG0380016317.01.T01:CDS 30.0%
ACTAACCATAAGAGTTAGTT+AGG - Chr3:81918725-81918744 MsG0380016317.01.T01:CDS 30.0%
CGTTAAAAGTGAATTAACGA+TGG - Chr3:81918355-81918374 MsG0380016317.01.T01:intron 30.0%
CTTCTTGTTCTTCTCATAAT+TGG + Chr3:81918149-81918168 None:intergenic 30.0%
TTCATTCTAGTAGTTTGACT+AGG - Chr3:81918847-81918866 MsG0380016317.01.T01:CDS 30.0%
TTGAACCTAACTAACTCTTA+TGG + Chr3:81918733-81918752 None:intergenic 30.0%
!! TATTTTCAGTATCGAACCAA+AGG - Chr3:81918799-81918818 MsG0380016317.01.T01:CDS 30.0%
AGACATCTACATGTATAGGT+AGG - Chr3:81918562-81918581 MsG0380016317.01.T01:intron 35.0%
CAACATCAAGAATCCACATA+CGG - Chr3:81918310-81918329 MsG0380016317.01.T01:intron 35.0%
CTTGTTCTTCTCATAATTGG+TGG + Chr3:81918146-81918165 None:intergenic 35.0%
! AGATACAGCATTTTGCAGTT+TGG - Chr3:81919027-81919046 MsG0380016317.01.T01:CDS 35.0%
! TTTTACCTTTGACTGAACTC+CGG + Chr3:81918672-81918691 None:intergenic 35.0%
AATCCACATACGGTTGTGAT+GGG - Chr3:81918320-81918339 MsG0380016317.01.T01:intron 40.0%
ATGGCTGATGAACGTTTAGT+GGG - Chr3:81918374-81918393 MsG0380016317.01.T01:intron 40.0%
ATTGTTAGTGTTTGTGGCTG+TGG + Chr3:81918429-81918448 None:intergenic 40.0%
CACCATGATGATCAAGAACA+TGG - Chr3:81918284-81918303 MsG0380016317.01.T01:intron 40.0%
GTCGAGACATCTACATGTAT+AGG - Chr3:81918558-81918577 MsG0380016317.01.T01:intron 40.0%
TGCCATGTTCTTGATCATCA+TGG + Chr3:81918289-81918308 None:intergenic 40.0%
TGTTCTTGATCATCATGGTG+TGG + Chr3:81918284-81918303 None:intergenic 40.0%
! AGAAGAACAAGAAGCATGAG+TGG - Chr3:81918155-81918174 MsG0380016317.01.T01:CDS 40.0%
! ATGATGGGATTCTAGGTTGT+TGG + Chr3:81918216-81918235 None:intergenic 40.0%
!! ATTTTGCAGTTTGGAGTGCA+GGG - Chr3:81919036-81919055 MsG0380016317.01.T01:CDS 40.0%
!! CACTGATTCTTCCTCATGAT+GGG + Chr3:81918231-81918250 None:intergenic 40.0%
!! TCACTGATTCTTCCTCATGA+TGG + Chr3:81918232-81918251 None:intergenic 40.0%
!! TCTAGGTTGTTGGTTTGATC+AGG + Chr3:81918206-81918225 None:intergenic 40.0%
ACAAGCAGCAGTAGAAGCTT+TGG + Chr3:81919158-81919177 None:intergenic 45.0%
AGTTACAGAGGCCACAAGTT+TGG + Chr3:81918523-81918542 None:intergenic 45.0%
CAACCTAGAATCCCATCATG+AGG - Chr3:81918217-81918236 MsG0380016317.01.T01:CDS 45.0%
CCACAAGTTTGGAGAAAGTG+AGG + Chr3:81918512-81918531 None:intergenic 45.0%
CCTCACTTTCTCCAAACTTG+TGG - Chr3:81918509-81918528 MsG0380016317.01.T01:intron 45.0%
GAAATCCGGAGTTCAGTCAA+AGG - Chr3:81918664-81918683 MsG0380016317.01.T01:intron 45.0%
GAATCCACATACGGTTGTGA+TGG - Chr3:81918319-81918338 MsG0380016317.01.T01:intron 45.0%
GATGGCTGATGAACGTTTAG+TGG - Chr3:81918373-81918392 MsG0380016317.01.T01:intron 45.0%
TACACGGTGGTCTTCTTTGT+TGG + Chr3:81919113-81919132 None:intergenic 45.0%
TACATGTAGATGTCTCGACC+AGG + Chr3:81918557-81918576 None:intergenic 45.0%
TCATGTGCACTCACACTCAA+AGG + Chr3:81918473-81918492 None:intergenic 45.0%
TGATCATCATGGTGTGGATG+TGG + Chr3:81918278-81918297 None:intergenic 45.0%
TGTAAACCCTACTCTGCCTA+GGG - Chr3:81918626-81918645 MsG0380016317.01.T01:intron 45.0%
! AGAACAAGAAGCATGAGTGG+TGG - Chr3:81918158-81918177 MsG0380016317.01.T01:CDS 45.0%
! CTTCCTCATGATGGGATTCT+AGG + Chr3:81918223-81918242 None:intergenic 45.0%
! CTTCTCATAATTGGTGGTCG+AGG + Chr3:81918140-81918159 None:intergenic 45.0%
!! AGGTTGTTGGTTTGATCAGG+TGG + Chr3:81918203-81918222 None:intergenic 45.0%
!! CATTTTGCAGTTTGGAGTGC+AGG - Chr3:81919035-81919054 MsG0380016317.01.T01:CDS 45.0%
AAGGCAGAGCCTTCGAAATC+CGG - Chr3:81918650-81918669 MsG0380016317.01.T01:intron 50.0%
ATCAGGTGGTTGCATGTCCA+CGG + Chr3:81918189-81918208 None:intergenic 50.0%
GACTGAACTCCGGATTTCGA+AGG + Chr3:81918662-81918681 None:intergenic 50.0%
GAGCCAAACGACAGTTACAG+AGG + Chr3:81918535-81918554 None:intergenic 50.0%
GTGTAAACCCTACTCTGCCT+AGG - Chr3:81918625-81918644 MsG0380016317.01.T01:intron 50.0%
GTTCCCATCACAACCGTATG+TGG + Chr3:81918326-81918345 None:intergenic 50.0%
TGGCCTCTGTAACTGTCGTT+TGG - Chr3:81918529-81918548 MsG0380016317.01.T01:intron 50.0%
! AGCAGTAGAAGCTTTGGCAG+TGG + Chr3:81919152-81919171 None:intergenic 50.0%
! TGTAACTGTCGTTTGGCTCC+TGG - Chr3:81918536-81918555 MsG0380016317.01.T01:intron 50.0%
TGGCTGTGGCTGTGTGTGAA+GGG + Chr3:81918415-81918434 None:intergenic 55.0%
! CATGAGTGGTGGTTTGTCCG+TGG - Chr3:81918169-81918188 MsG0380016317.01.T01:CDS 55.0%
ACCCTACTCTGCCTAGGGCA+AGG - Chr3:81918631-81918650 MsG0380016317.01.T01:intron 60.0%
CCGTGCACGGTGGTGATACA+CGG + Chr3:81919129-81919148 None:intergenic 60.0%
CCGTGTATCACCACCGTGCA+CGG - Chr3:81919126-81919145 MsG0380016317.01.T01:CDS 60.0%
GCCTTGCCCTAGGCAGAGTA+GGG + Chr3:81918635-81918654 None:intergenic 60.0%
GTGGCTGTGGCTGTGTGTGA+AGG + Chr3:81918416-81918435 None:intergenic 60.0%
TGCACGGTGGTGATACACGG+TGG + Chr3:81919126-81919145 None:intergenic 60.0%
TGCCTTGCCCTAGGCAGAGT+AGG + Chr3:81918636-81918655 None:intergenic 60.0%
TGGTGTGGATGTGGCTGCAG+AGG + Chr3:81918269-81918288 None:intergenic 60.0%
CGAAGGCTCTGCCTTGCCCT+AGG + Chr3:81918645-81918664 None:intergenic 65.0%
GCTTTGGCAGTGGCCGTGCA+CGG + Chr3:81919142-81919161 None:intergenic 65.0%
TTGGCAGTGGCCGTGCACGG+TGG + Chr3:81919139-81919158 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 81918119 81919183 81918119 ID=MsG0380016317.01;Name=MsG0380016317.01
Chr3 mRNA 81918119 81919183 81918119 ID=MsG0380016317.01.T01;Parent=MsG0380016317.01;Name=MsG0380016317.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|206
Chr3 exon 81918723 81919183 81918723 ID=MsG0380016317.01.T01:exon:11667;Parent=MsG0380016317.01.T01
Chr3 exon 81918119 81918278 81918119 ID=MsG0380016317.01.T01:exon:11666;Parent=MsG0380016317.01.T01
Chr3 CDS 81918723 81919183 81918723 ID=MsG0380016317.01.T01:cds;Parent=MsG0380016317.01.T01
Chr3 CDS 81918119 81918278 81918119 ID=MsG0380016317.01.T01:cds;Parent=MsG0380016317.01.T01
Gene Sequence

>MsG0380016317.01.T01

ATGTTGCTTGGAAAACGTCCTCGACCACCAATTATGAGAAGAACAAGAAGCATGAGTGGTGGTTTGTCCGTGGACATGCAACCACCTGATCAAACCAACAACCTAGAATCCCATCATGAGGAAGAATCAGTGATGTCTCATCAGAATCCTCTGCAGCCACATCCACACCATGATGATCAAGAACATGGCATCAACATCAAGAATCCACATACGGTTGTGATGGGAACAGAAACACACGTTAAAAGTGAATTAACGATGGCTGATGAACGTTTAGTGGGTAGTGCAGTCATGTTCCCTTCACACACAGCCACAGCCACAAACACTAACAATATTATTAATCATAATAATAATCCTTTGAGTGTGAGTGCACATGATGTCATACATAGTACACCTCACTTTCTCCAAACTTGTGGCCTCTGTAACTGTCGTTTGGCTCCTGGTCGAGACATCTACATGTATAGGGGAGATACAGCATTTTGCAGTTTGGAGTGCAGGGAGCAACAAATAAAACAAGACAAAAGAAAAGAAAAGTGGAAGATGGCGTTCACCAACAAAGAAGACCACCGTGTATCACCACCGTGCACGGCCACTGCCAAAGCTTCTACTGCTGCTTGTACTTGA

Protein sequence

>MsG0380016317.01.T01

MLLGKRPRPPIMRRTRSMSGGLSVDMQPPDQTNNLESHHEEESVMSHQNPLQPHPHHDDQEHGINIKNPHTVVMGTETHVKSELTMADERLVGSAVMFPSHTATATNTNNIINHNNNPLSVSAHDVIHSTPHFLQTCGLCNCRLAPGRDIYMYRGDTAFCSLECREQQIKQDKRKEKWKMAFTNKEDHRVSPPCTATAKASTAACT*