Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016792.01.T01 | RHN69890.1 | 96.364 | 110 | 4 | 0 | 1 | 110 | 262 | 371 | 1.33E-68 | 220 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016792.01.T01 | F4J8V5 | 48.315 | 89 | 38 | 3 | 14 | 101 | 526 | 607 | 9.68E-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016792.01.T01 | A0A396IWQ4 | 96.364 | 110 | 4 | 0 | 1 | 110 | 262 | 371 | 6.36e-69 | 220 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380016792.01 | MsG0380017793.01 | 0.815341 | 9.715681e-52 | 6.680733e-49 |
| MsG0380016792.01 | MsG0480018226.01 | 0.812151 | 4.898087e-51 | 3.090320e-48 |
| MsG0380016792.01 | MsG0480018670.01 | 0.803447 | 3.470935e-49 | 1.744630e-46 |
| MsG0380016792.01 | MsG0580025564.01 | 0.808460 | 3.064213e-50 | 1.753111e-47 |
| MsG0380016792.01 | MsG0580025827.01 | 0.802101 | 6.581289e-49 | 3.196233e-46 |
| MsG0380016792.01 | MsG0580030071.01 | 0.816573 | 5.157649e-52 | 3.668174e-49 |
| MsG0380016792.01 | MsG0680030907.01 | 0.821403 | 4.113981e-53 | 3.343638e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016792.01.T01 | MTR_3g095570 | 96.364 | 110 | 4 | 0 | 1 | 110 | 524 | 633 | 1.27e-69 | 219 |
| MsG0380016792.01.T01 | MTR_8g093050 | 49.640 | 139 | 32 | 5 | 1 | 110 | 454 | 583 | 1.27e-27 | 105 |
| MsG0380016792.01.T01 | MTR_8g093050 | 49.640 | 139 | 32 | 5 | 1 | 110 | 428 | 557 | 1.37e-27 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016792.01.T01 | AT3G18770 | 48.315 | 89 | 38 | 3 | 14 | 101 | 526 | 607 | 9.87e-15 | 68.9 |
| MsG0380016792.01.T01 | AT3G49590 | 40.000 | 100 | 41 | 2 | 1 | 100 | 525 | 605 | 5.52e-11 | 58.2 |
| MsG0380016792.01.T01 | AT3G49590 | 40.000 | 100 | 41 | 2 | 1 | 100 | 510 | 590 | 5.84e-11 | 58.2 |
| MsG0380016792.01.T01 | AT3G49590 | 40.000 | 100 | 41 | 2 | 1 | 100 | 510 | 590 | 5.84e-11 | 58.2 |
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTTGGCTCTCTAGAATC+TGG | 0.350434 | 3:+87971562 | None:intergenic |
| CCAGTGAGCACAATGTCTTA+TGG | 0.355804 | 3:-87971540 | MsG0380016792.01.T01:CDS |
| ACATTGTGCTCACTGGTGTT+TGG | 0.395568 | 3:+87971547 | None:intergenic |
| CAGTGAGCACAATGTCTTAT+GGG | 0.398369 | 3:-87971539 | MsG0380016792.01.T01:CDS |
| GCACCTTGAGACTTTCTGAT+TGG | 0.405605 | 3:+87971714 | None:intergenic |
| CACTGGTGAAAACTTGTCTC+AGG | 0.410890 | 3:-87971631 | MsG0380016792.01.T01:CDS |
| GCACAATGTCTTATGGGAAT+AGG | 0.432338 | 3:-87971533 | MsG0380016792.01.T01:CDS |
| GAAAACAACAAGTGATGCAT+TGG | 0.476214 | 3:-87971511 | MsG0380016792.01.T01:CDS |
| TGGCTCTCTAGAATCTGGTT+CGG | 0.476523 | 3:+87971567 | None:intergenic |
| CCATAAGACATTGTGCTCAC+TGG | 0.510597 | 3:+87971540 | None:intergenic |
| ATGACTCAACTTCTTGAAGC+TGG | 0.533846 | 3:-87971747 | MsG0380016792.01.T01:CDS |
| CTGCATCAAGACTTCTCCAC+TGG | 0.558770 | 3:-87971648 | MsG0380016792.01.T01:CDS |
| TTATTACTTATGCCACGTGA+TGG | 0.594204 | 3:-87971456 | MsG0380016792.01.T01:CDS |
| AAGTCTCAAGGTGCCGCTGT+TGG | 0.594638 | 3:-87971705 | MsG0380016792.01.T01:CDS |
| GTGGAGAAGTCTTGATGCAG+AGG | 0.607632 | 3:+87971651 | None:intergenic |
| ACTGGTGAAAACTTGTCTCA+GGG | 0.609755 | 3:-87971630 | MsG0380016792.01.T01:CDS |
| TTTCCAATCAGAAAGTCTCA+AGG | 0.615481 | 3:-87971717 | MsG0380016792.01.T01:CDS |
| TGTCGCTTACTGCCATCACG+TGG | 0.644221 | 3:+87971444 | None:intergenic |
| CTCAACTTCTTGAAGCTGGT+AGG | 0.648198 | 3:-87971743 | MsG0380016792.01.T01:CDS |
| ATGCACAAGTGCACCAACAG+CGG | 0.706901 | 3:+87971692 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTGCTCTTCAAGTTTTC+AGG | + | Chr3:87971587-87971606 | None:intergenic | 30.0% |
| GAAAACAACAAGTGATGCAT+TGG | - | Chr3:87971673-87971692 | MsG0380016792.01.T01:CDS | 35.0% | |
| TTATTACTTATGCCACGTGA+TGG | - | Chr3:87971728-87971747 | MsG0380016792.01.T01:CDS | 35.0% | |
| TTTCCAATCAGAAAGTCTCA+AGG | - | Chr3:87971467-87971486 | MsG0380016792.01.T01:CDS | 35.0% | |
| ACTGGTGAAAACTTGTCTCA+GGG | - | Chr3:87971554-87971573 | MsG0380016792.01.T01:CDS | 40.0% | |
| ATGACTCAACTTCTTGAAGC+TGG | - | Chr3:87971437-87971456 | MsG0380016792.01.T01:CDS | 40.0% | |
| CAGTGAGCACAATGTCTTAT+GGG | - | Chr3:87971645-87971664 | MsG0380016792.01.T01:CDS | 40.0% | |
| GCACAATGTCTTATGGGAAT+AGG | - | Chr3:87971651-87971670 | MsG0380016792.01.T01:CDS | 40.0% | |
| !! | TGCTCTTCAAGTTTTCAGGA+CGG | + | Chr3:87971583-87971602 | None:intergenic | 40.0% |
| ACATTGTGCTCACTGGTGTT+TGG | + | Chr3:87971640-87971659 | None:intergenic | 45.0% | |
| CACTGGTGAAAACTTGTCTC+AGG | - | Chr3:87971553-87971572 | MsG0380016792.01.T01:CDS | 45.0% | |
| CCAGTGAGCACAATGTCTTA+TGG | - | Chr3:87971644-87971663 | MsG0380016792.01.T01:CDS | 45.0% | |
| CCATAAGACATTGTGCTCAC+TGG | + | Chr3:87971647-87971666 | None:intergenic | 45.0% | |
| CTCAACTTCTTGAAGCTGGT+AGG | - | Chr3:87971441-87971460 | MsG0380016792.01.T01:CDS | 45.0% | |
| GCACCTTGAGACTTTCTGAT+TGG | + | Chr3:87971473-87971492 | None:intergenic | 45.0% | |
| ! | CTGAGACAAGTTTTCACCAG+TGG | + | Chr3:87971555-87971574 | None:intergenic | 45.0% |
| !! | GCTCTTCAAGTTTTCAGGAC+GGG | + | Chr3:87971582-87971601 | None:intergenic | 45.0% |
| !! | GTGTTTGGCTCTCTAGAATC+TGG | + | Chr3:87971625-87971644 | None:intergenic | 45.0% |
| !! | TGGCTCTCTAGAATCTGGTT+CGG | + | Chr3:87971620-87971639 | None:intergenic | 45.0% |
| ATGCACAAGTGCACCAACAG+CGG | + | Chr3:87971495-87971514 | None:intergenic | 50.0% | |
| CTGCATCAAGACTTCTCCAC+TGG | - | Chr3:87971536-87971555 | MsG0380016792.01.T01:CDS | 50.0% | |
| GTGGAGAAGTCTTGATGCAG+AGG | + | Chr3:87971536-87971555 | None:intergenic | 50.0% | |
| ! | TGTCGCTTACTGCCATCACG+TGG | + | Chr3:87971743-87971762 | None:intergenic | 55.0% |
| !! | AAGTCTCAAGGTGCCGCTGT+TGG | - | Chr3:87971479-87971498 | MsG0380016792.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 87971437 | 87971769 | 87971437 | ID=MsG0380016792.01;Name=MsG0380016792.01 |
| Chr3 | mRNA | 87971437 | 87971769 | 87971437 | ID=MsG0380016792.01.T01;Parent=MsG0380016792.01;Name=MsG0380016792.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|110 |
| Chr3 | exon | 87971437 | 87971769 | 87971437 | ID=MsG0380016792.01.T01:exon:22408;Parent=MsG0380016792.01.T01 |
| Chr3 | CDS | 87971437 | 87971769 | 87971437 | ID=MsG0380016792.01.T01:cds;Parent=MsG0380016792.01.T01 |
| Gene Sequence |
| Protein sequence |
