AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025564.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025564.01.T01 MTR_5g030600 72.455 167 11 4 1 133 509 674 5.20e-74 233
MsG0580025564.01.T01 MTR_7g037650 91.765 85 6 1 7 91 155 238 9.45e-48 160
MsG0580025564.01.T01 MTR_4g094595 27.027 148 71 2 5 116 819 965 7.97e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025564.01.T01 AT4G18240 32.584 89 59 1 5 93 870 957 6.72e-13 64.7

Find 27 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTCCGCAGTTTATATTTA+TGG 0.032226 5:-20561643 MsG0580025564.01.T01:CDS
ATATGTAGCTCAAAGCTTTA+AGG 0.154547 5:-20560833 MsG0580025564.01.T01:intron
CATTATTCTTCGTTTCCATT+TGG 0.324608 5:+20559947 None:intergenic
CAAATGGAAACGAAGAATAA+TGG 0.372793 5:-20559946 MsG0580025564.01.T01:CDS
GGCACTAGAATCACTTCATA+TGG 0.401474 5:-20561592 MsG0580025564.01.T01:CDS
ATTCTTCGTTTCCATTTGGA+TGG 0.413455 5:+20559951 None:intergenic
ATCACTTCATATGGAATTCA+AGG 0.413713 5:-20561583 MsG0580025564.01.T01:intron
GAATCAGAGTCAACTGCTAC+TGG 0.439908 5:+20560798 None:intergenic
CAATGGCACATGATTTATCA+TGG 0.442232 5:-20559920 MsG0580025564.01.T01:CDS
ATTCAAGTCTGGTCTTTCAC+TGG 0.460075 5:+20561617 None:intergenic
TCTAGTGCCTGATTCAAGTC+TGG 0.461256 5:+20561606 None:intergenic
TGAAAGACCAGACTTGAATC+AGG 0.492845 5:-20561613 MsG0580025564.01.T01:CDS
TAGCTCAAAGCTTTAAGGTA+TGG 0.509482 5:-20560828 MsG0580025564.01.T01:CDS
TTCTTCGTTTCCATTTGGAT+GGG 0.513572 5:+20559952 None:intergenic
AGAGGAGTAACCCATCCAAA+TGG 0.524697 5:-20559962 MsG0580025564.01.T01:CDS
CTTACTGGGGTTTCATCAGT+AGG 0.525772 5:+20561168 None:intergenic
CTATCGCATTTATTATTCGC+AGG 0.527294 5:-20561225 MsG0580025564.01.T01:CDS
GCTATAAAGAATATGTGAAG+TGG 0.567967 5:-20559858 MsG0580025564.01.T01:exon
ACGAAGAATAATGGAAGCAA+TGG 0.575742 5:-20559937 MsG0580025564.01.T01:CDS
TTACTCAAGAGAAGGAAAGC+CGG 0.582749 5:-20559689 MsG0580025564.01.T01:three_prime_UTR
AAGTAATCTTACTCAAGAGA+AGG 0.590473 5:-20559697 MsG0580025564.01.T01:three_prime_UTR
CTATAAAGAATATGTGAAGT+GGG 0.591922 5:-20559857 MsG0580025564.01.T01:exon
AATGGCACATGATTTATCAT+GGG 0.600151 5:-20559919 MsG0580025564.01.T01:CDS
TCGTGAGGCTAACAAAGTTG+CGG 0.633006 5:+20559657 None:intergenic
GCACATGATTTATCATGGGA+TGG 0.639732 5:-20559915 MsG0580025564.01.T01:CDS
GTCTCCATAAATATAAACTG+CGG 0.694325 5:+20561639 None:intergenic
TGAATGCTACGAAGTTCATG+TGG 0.756833 5:-20559892 MsG0580025564.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTCTAAATAAATATTACTAT+TGG + Chr5:20559982-20560001 None:intergenic 15.0%
!!! TAAACAAATTTGATTTTAAG+CGG - Chr5:20560272-20560291 MsG0580025564.01.T01:intron 15.0%
!! AAGTAGAAGTGATTAATATA+TGG + Chr5:20561204-20561223 None:intergenic 20.0%
!! ATAAGTCACAAATATTCTTA+AGG + Chr5:20560566-20560585 None:intergenic 20.0%
!! GTGTACACAATATTAATTAA+GGG + Chr5:20560941-20560960 None:intergenic 20.0%
!! TGTGTACACAATATTAATTA+AGG + Chr5:20560942-20560961 None:intergenic 20.0%
!!! ATTAGTTATTGACTTTGATA+TGG - Chr5:20561328-20561347 MsG0580025564.01.T01:intron 20.0%
!!! ATTCACTATTTTGAGATATA+TGG - Chr5:20561528-20561547 MsG0580025564.01.T01:intron 20.0%
!!! TTAGTTATTGACTTTGATAT+GGG - Chr5:20561329-20561348 MsG0580025564.01.T01:intron 20.0%
! AAGTTAATCAACAATACCTT+TGG - Chr5:20560694-20560713 MsG0580025564.01.T01:intron 25.0%
! AATGAAATGTATGTAGTTTC+TGG - Chr5:20560747-20560766 MsG0580025564.01.T01:intron 25.0%
! CATACATTTCATTTCATCAA+TGG + Chr5:20560740-20560759 None:intergenic 25.0%
! CATTTATGACAGTTGTTAAT+AGG - Chr5:20560980-20560999 MsG0580025564.01.T01:intron 25.0%
! CTACTTTAGTAAACTATCAT+TGG - Chr5:20561218-20561237 MsG0580025564.01.T01:CDS 25.0%
! CTATAAAGAATATGTGAAGT+GGG - Chr5:20561460-20561479 MsG0580025564.01.T01:intron 25.0%
! GATTAATATATGGATCGTAT+TGG + Chr5:20561194-20561213 None:intergenic 25.0%
! GTTTATGAAGTTCCTATTAA+AGG + Chr5:20560647-20560666 None:intergenic 25.0%
! TGTACATATTCATTCATTGA+TGG - Chr5:20561037-20561056 MsG0580025564.01.T01:intron 25.0%
! TTAACAAGTACTAATAGCTT+CGG - Chr5:20559857-20559876 MsG0580025564.01.T01:exon 25.0%
! TTAATAGGCAATAGTTAACT+GGG - Chr5:20560995-20561014 MsG0580025564.01.T01:intron 25.0%
!! ACAATGAAAATCAAGAGTAT+AGG + Chr5:20559764-20559783 None:intergenic 25.0%
!! CAATGAAAATCAAGAGTATA+GGG + Chr5:20559763-20559782 None:intergenic 25.0%
!!! CAAGTTGATAAAATCAAAGT+TGG + Chr5:20560205-20560224 None:intergenic 25.0%
AAGTAATCTTACTCAAGAGA+AGG - Chr5:20561620-20561639 MsG0580025564.01.T01:CDS 30.0%
AATGGCACATGATTTATCAT+GGG - Chr5:20561398-20561417 MsG0580025564.01.T01:intron 30.0%
ATATGTAGCTCAAAGCTTTA+AGG - Chr5:20560484-20560503 MsG0580025564.01.T01:intron 30.0%
ATCACTTCATATGGAATTCA+AGG - Chr5:20559734-20559753 MsG0580025564.01.T01:three_prime_UTR 30.0%
ATGTGAGTGTTACACAAATA+TGG + Chr5:20560601-20560620 None:intergenic 30.0%
ATTTCATTTCATCAATGGCA+AGG + Chr5:20560735-20560754 None:intergenic 30.0%
CAAATGGAAACGAAGAATAA+TGG - Chr5:20561371-20561390 MsG0580025564.01.T01:intron 30.0%
CATTATTCTTCGTTTCCATT+TGG + Chr5:20561373-20561392 None:intergenic 30.0%
GCTATAAAGAATATGTGAAG+TGG - Chr5:20561459-20561478 MsG0580025564.01.T01:intron 30.0%
GTCTCCATAAATATAAACTG+CGG + Chr5:20559681-20559700 None:intergenic 30.0%
GTTAATAGGCAATAGTTAAC+TGG - Chr5:20560994-20561013 MsG0580025564.01.T01:intron 30.0%
TAATAGGCAATAGTTAACTG+GGG - Chr5:20560996-20561015 MsG0580025564.01.T01:intron 30.0%
TCACATCATTCATATTTGCT+CGG - Chr5:20560615-20560634 MsG0580025564.01.T01:intron 30.0%
!!! GACACTTATTTTGAAACAGA+GGG + Chr5:20560837-20560856 None:intergenic 30.0%
AATAGCACAAATAGTCGATG+TGG - Chr5:20560391-20560410 MsG0580025564.01.T01:intron 35.0%
ACCTTAAACGACCCTTAATT+TGG - Chr5:20561083-20561102 MsG0580025564.01.T01:intron 35.0%
AGGTTCTTTCACATAGATAG+AGG + Chr5:20561067-20561086 None:intergenic 35.0%
AGTTGGATAATCAGAACACA+AGG + Chr5:20560188-20560207 None:intergenic 35.0%
ATAATCAGAACACAAGGGAA+TGG + Chr5:20560182-20560201 None:intergenic 35.0%
ATAGCACAAATAGTCGATGT+GGG - Chr5:20560392-20560411 MsG0580025564.01.T01:intron 35.0%
ATCTTGTGTCTAACACATGT+AGG - Chr5:20561149-20561168 MsG0580025564.01.T01:intron 35.0%
ATTCTTCGTTTCCATTTGGA+TGG + Chr5:20561369-20561388 None:intergenic 35.0%
CAATGGCACATGATTTATCA+TGG - Chr5:20561397-20561416 MsG0580025564.01.T01:intron 35.0%
CACATCATTCATATTTGCTC+GGG - Chr5:20560616-20560635 MsG0580025564.01.T01:intron 35.0%
CTATCGCATTTATTATTCGC+AGG - Chr5:20560092-20560111 MsG0580025564.01.T01:intron 35.0%
GTAAACTATCATTGGTCATG+TGG - Chr5:20561226-20561245 MsG0580025564.01.T01:CDS 35.0%
GTTGGATAATCAGAACACAA+GGG + Chr5:20560187-20560206 None:intergenic 35.0%
TAATCAGAACACAAGGGAAT+GGG + Chr5:20560181-20560200 None:intergenic 35.0%
TCATTCATTCTGCATGACAA+TGG + Chr5:20559895-20559914 None:intergenic 35.0%
TCATTCCATTCAAAATCTGC+AGG - Chr5:20560025-20560044 MsG0580025564.01.T01:intron 35.0%
TCCAAATTAAGGGTCGTTTA+AGG + Chr5:20561087-20561106 None:intergenic 35.0%
TGTTATAAATTCCTTGCGAC+TGG - Chr5:20559794-20559813 MsG0580025564.01.T01:three_prime_UTR 35.0%
TTCTTCGTTTCCATTTGGAT+GGG + Chr5:20561368-20561387 None:intergenic 35.0%
! AAACGACCCTTAATTTGGAA+GGG - Chr5:20561088-20561107 MsG0580025564.01.T01:intron 35.0%
! ACGAAGAATAATGGAAGCAA+TGG - Chr5:20561380-20561399 MsG0580025564.01.T01:intron 35.0%
! ATCATCCTGCAGATTTTGAA+TGG + Chr5:20560033-20560052 None:intergenic 35.0%
! GCAAAAACACAGAGAGATTT+TGG + Chr5:20561277-20561296 None:intergenic 35.0%
! TAAACGACCCTTAATTTGGA+AGG - Chr5:20561087-20561106 MsG0580025564.01.T01:intron 35.0%
!! ACACAATCATCGAGTATTGA+TGG - Chr5:20559827-20559846 MsG0580025564.01.T01:three_prime_UTR 35.0%
!! GTGTAGATTTTAGAGGTCAA+TGG - Chr5:20560312-20560331 MsG0580025564.01.T01:intron 35.0%
!! TAGCTCAAAGCTTTAAGGTA+TGG - Chr5:20560489-20560508 MsG0580025564.01.T01:intron 35.0%
!!! CGACACTTATTTTGAAACAG+AGG + Chr5:20560838-20560857 None:intergenic 35.0%
AATCAGAACACAAGGGAATG+GGG + Chr5:20560180-20560199 None:intergenic 40.0%
ATGTAGGTGTCAACATGTGT+TGG - Chr5:20561165-20561184 MsG0580025564.01.T01:intron 40.0%
CAAATAGTCGATGTGGGATA+TGG - Chr5:20560398-20560417 MsG0580025564.01.T01:intron 40.0%
CGCTCCGCAGTTTATATTTA+TGG - Chr5:20559674-20559693 MsG0580025564.01.T01:three_prime_UTR 40.0%
CTTCATAAACCATGACCAAG+AGG - Chr5:20560657-20560676 MsG0580025564.01.T01:intron 40.0%
GCACATGATTTATCATGGGA+TGG - Chr5:20561402-20561421 MsG0580025564.01.T01:intron 40.0%
GGCACTAGAATCACTTCATA+TGG - Chr5:20559725-20559744 MsG0580025564.01.T01:three_prime_UTR 40.0%
TGAAAGACCAGACTTGAATC+AGG - Chr5:20559704-20559723 MsG0580025564.01.T01:three_prime_UTR 40.0%
TGAATGCTACGAAGTTCATG+TGG - Chr5:20561425-20561444 MsG0580025564.01.T01:intron 40.0%
TGACTTTGATATGGGTGTAG+AGG - Chr5:20561337-20561356 MsG0580025564.01.T01:intron 40.0%
TTACTCAAGAGAAGGAAAGC+CGG - Chr5:20561628-20561647 MsG0580025564.01.T01:CDS 40.0%
TTGAGTACTTAGTTGCCTCT+TGG + Chr5:20560675-20560694 None:intergenic 40.0%
! ACCTTTGGAAACATGTCACT+TGG - Chr5:20560709-20560728 MsG0580025564.01.T01:intron 40.0%
! ATTCAAGTCTGGTCTTTCAC+TGG + Chr5:20559703-20559722 None:intergenic 40.0%
! TTTAAGCGGAGATTTTCACG+TGG - Chr5:20560286-20560305 MsG0580025564.01.T01:intron 40.0%
!! TCATCGAGTATTGATGGCTT+TGG - Chr5:20559833-20559852 MsG0580025564.01.T01:three_prime_UTR 40.0%
!!! AAAGTTGCGGATGCTTTTTC+CGG + Chr5:20561650-20561669 None:intergenic 40.0%
ACTGCCTCCCTTCCAAATTA+AGG + Chr5:20561098-20561117 None:intergenic 45.0%
ACTTAGTTGCCTCTTGGTCA+TGG + Chr5:20560669-20560688 None:intergenic 45.0%
AGAGGAGTAACCCATCCAAA+TGG - Chr5:20561355-20561374 MsG0580025564.01.T01:intron 45.0%
CTGCCTCCCTTCCAAATTAA+GGG + Chr5:20561097-20561116 None:intergenic 45.0%
CTTACTGGGGTTTCATCAGT+AGG + Chr5:20560152-20560171 None:intergenic 45.0%
GAATCAGAGTCAACTGCTAC+TGG + Chr5:20560522-20560541 None:intergenic 45.0%
GCCAAGTGACATGTTTCCAA+AGG + Chr5:20560713-20560732 None:intergenic 45.0%
TCTAGTGCCTGATTCAAGTC+TGG + Chr5:20559714-20559733 None:intergenic 45.0%
! CTGCGGAGCGTTAATAATCA+TGG + Chr5:20559664-20559683 None:intergenic 45.0%
! GTGGCACGTGTAGATTTTAG+AGG - Chr5:20560305-20560324 MsG0580025564.01.T01:intron 45.0%
AGAACACAAGGGAATGGGGA+TGG + Chr5:20560176-20560195 None:intergenic 50.0%
GCTCGGGTGACACCTTTAAT+AGG - Chr5:20560632-20560651 MsG0580025564.01.T01:intron 50.0%
! CGACCCTTAATTTGGAAGGG+AGG - Chr5:20561091-20561110 MsG0580025564.01.T01:intron 50.0%
! GAATGGGGATGGACTTACTG+GGG + Chr5:20560165-20560184 None:intergenic 50.0%
! GGAATGGGGATGGACTTACT+GGG + Chr5:20560166-20560185 None:intergenic 50.0%
! GGGAATGGGGATGGACTTAC+TGG + Chr5:20560167-20560186 None:intergenic 55.0%
TGCACGTGCAACCAGTCGCA+AGG + Chr5:20559808-20559827 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 20559663 20561676 20559663 ID=MsG0580025564.01;Name=MsG0580025564.01
Chr5 mRNA 20559663 20561676 20559663 ID=MsG0580025564.01.T01;Parent=MsG0580025564.01;Name=MsG0580025564.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0.66|0.5|1|0|0.5|4|200|133
Chr5 exon 20559663 20559982 20559663 ID=MsG0580025564.01.T01:exon:8978;Parent=MsG0580025564.01.T01
Chr5 exon 20560779 20560847 20560779 ID=MsG0580025564.01.T01:exon:8977;Parent=MsG0580025564.01.T01
Chr5 exon 20561173 20561292 20561173 ID=MsG0580025564.01.T01:exon:8976;Parent=MsG0580025564.01.T01
Chr5 exon 20561584 20561676 20561584 ID=MsG0580025564.01.T01:exon:8975;Parent=MsG0580025564.01.T01
Chr5 CDS 20561584 20561676 20561584 ID=MsG0580025564.01.T01:cds;Parent=MsG0580025564.01.T01
Chr5 CDS 20561173 20561292 20561173 ID=MsG0580025564.01.T01:cds;Parent=MsG0580025564.01.T01
Chr5 CDS 20560779 20560847 20560779 ID=MsG0580025564.01.T01:cds;Parent=MsG0580025564.01.T01
Chr5 CDS 20559863 20559982 20559863 ID=MsG0580025564.01.T01:cds;Parent=MsG0580025564.01.T01
Chr5 three_prime_UTR 20559663 20559862 20559663 ID=MsG0580025564.01.T01:three_prime_utr;Parent=MsG0580025564.01.T01
Gene Sequence

>MsG0580025564.01.T01

ATGATTATTAACGCTCCGCAGTTTATATTTATGGAGACCAGTGAAAGACCAGACTTGAATCAGGCACTAGAATCACTTCATATGGAATTCAAGGATGATGATGATAATATTAAATTTGTGCGTTCATATGATGAAGCTCTATCGCATTTATTATTCGCAGGATCAGACATTATATTATGTCGATCTTTTCATGATCCTACTGATGAAACCCCACTCAAAGCTTTAAGGTATGGAGCAGTTCCAGTAGCAGTTGACTCTGATTCTCACACAAACAGTGTAGAAAGGAGTAACCCATCCAAATGGAAACGAAGAATAATGGAAGCAATGGCACATGATTTATCATGGGATGGTGAATGCTACGAAGTTCATGTGGCAGCTTACTCAGCTATAAAGAATATGTGA

Protein sequence

>MsG0580025564.01.T01

MIINAPQFIFMETSERPDLNQALESLHMEFKDDDDNIKFVRSYDEALSHLLFAGSDIILCRSFHDPTDETPLKALRYGAVPVAVDSDSHTNSVERSNPSKWKRRIMEAMAHDLSWDGECYEVHVAAYSAIKNM*