AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016838.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016838.01.T01 MTR_3g096330 93.252 163 7 2 1 162 616 775 4.72e-97 296
MsG0380016838.01.T01 MTR_8g007300 63.830 141 50 1 2 141 66 206 7.39e-58 180
MsG0380016838.01.T01 MTR_1g060120 47.826 115 60 0 3 117 72 186 2.05e-34 120
MsG0380016838.01.T01 MTR_1g085500 48.148 108 56 0 13 120 73 180 5.38e-31 111
MsG0380016838.01.T01 MTR_1g112200 47.748 111 58 0 7 117 62 172 1.11e-29 108
MsG0380016838.01.T01 MTR_1g085140 41.830 153 72 3 3 138 63 215 1.55e-29 107
MsG0380016838.01.T01 MTR_7g111240 38.562 153 77 3 3 138 59 211 1.83e-28 105
MsG0380016838.01.T01 MTR_7g111240 38.562 153 77 3 3 138 56 208 1.90e-28 104
MsG0380016838.01.T01 MTR_2g030855 40.132 152 77 3 8 152 64 208 1.52e-27 102
MsG0380016838.01.T01 MTR_2g031270 40.132 152 77 3 8 152 65 209 2.44e-27 102
MsG0380016838.01.T01 MTR_1g023770 43.609 133 68 3 8 133 61 193 3.34e-27 101
MsG0380016838.01.T01 MTR_2g041570 42.735 117 60 1 12 121 59 175 2.64e-26 99.4
MsG0380016838.01.T01 MTR_2g041580 42.735 117 60 1 12 121 59 175 3.24e-26 99.0
MsG0380016838.01.T01 MTR_5g036570 46.552 116 60 2 3 116 66 181 4.11e-26 99.0
MsG0380016838.01.T01 MTR_2g041550 42.478 113 58 1 12 117 59 171 2.10e-25 97.1
MsG0380016838.01.T01 MTR_8g020630 41.593 113 64 2 5 116 58 169 4.18e-24 93.6
MsG0380016838.01.T01 MTR_2g030895 38.994 159 81 5 3 152 23 174 4.60e-24 92.8
MsG0380016838.01.T01 MTR_1g079490 41.860 129 67 2 13 133 73 201 7.69e-24 93.2
MsG0380016838.01.T01 MTR_2g036120 44.000 100 55 1 16 115 70 168 1.00e-23 92.8
MsG0380016838.01.T01 MTR_8g020650 41.071 112 64 2 6 116 59 169 1.35e-23 92.4
MsG0380016838.01.T01 MTR_6g005340 41.606 137 72 3 5 133 63 199 1.38e-23 92.4
MsG0380016838.01.T01 MTR_8g020590 41.000 100 59 0 22 121 74 173 2.27e-23 91.7
MsG0380016838.01.T01 MTR_6g005360 42.188 128 67 2 13 133 72 199 2.50e-23 92.0
MsG0380016838.01.T01 MTR_2g030865 39.865 148 74 4 13 152 34 174 2.59e-23 90.9
MsG0380016838.01.T01 MTR_6g005310 42.188 128 67 2 13 133 72 199 2.81e-23 91.7
MsG0380016838.01.T01 MTR_6g005330 42.188 128 67 2 13 133 72 199 3.48e-23 91.7
MsG0380016838.01.T01 MTR_4g017040 45.370 108 56 1 13 117 72 179 3.87e-23 91.3
MsG0380016838.01.T01 MTR_4g017050 42.975 121 65 2 1 117 59 179 4.12e-23 91.3
MsG0380016838.01.T01 MTR_5g046410 45.370 108 56 1 13 117 72 179 4.30e-23 91.3
MsG0380016838.01.T01 MTR_5g046430 45.370 108 56 1 13 117 72 179 4.30e-23 91.3
MsG0380016838.01.T01 MTR_4g017030 45.370 108 56 1 13 117 72 179 4.30e-23 91.3
MsG0380016838.01.T01 MTR_8g020770 47.000 100 52 1 15 114 72 170 4.50e-23 90.9
MsG0380016838.01.T01 MTR_8g020610 40.196 102 61 0 15 116 66 167 5.80e-23 90.5
MsG0380016838.01.T01 MTR_2g044040 44.000 100 55 1 16 115 70 168 1.01e-22 90.1
MsG0380016838.01.T01 MTR_2g019250 45.370 108 56 1 13 117 72 179 1.31e-22 90.1
MsG0380016838.01.T01 MTR_2g045100 42.000 100 57 1 16 115 70 168 1.91e-22 89.4
MsG0380016838.01.T01 MTR_7g011340 44.898 98 53 1 18 115 72 168 2.69e-22 89.0
MsG0380016838.01.T01 MTR_0284s0010 43.434 99 55 1 17 115 71 168 5.77e-22 88.2
MsG0380016838.01.T01 MTR_8g020670 40.179 112 65 2 6 116 59 169 7.94e-22 87.8
MsG0380016838.01.T01 MTR_3g019710 43.689 103 57 1 20 122 74 175 1.18e-21 87.4
MsG0380016838.01.T01 MTR_8g020763 38.053 113 69 1 9 121 44 155 1.47e-21 86.7
MsG0380016838.01.T01 MTR_6g005380 42.593 108 59 1 13 117 72 179 1.62e-21 87.0
MsG0380016838.01.T01 MTR_4g010340 44.545 110 52 4 13 115 57 164 1.80e-21 86.7
MsG0380016838.01.T01 MTR_3g019650 42.718 103 58 1 20 122 73 174 2.90e-21 86.3
MsG0380016838.01.T01 MTR_3g019680 42.718 103 58 1 20 122 73 174 3.15e-21 86.3
MsG0380016838.01.T01 MTR_6g005350 46.154 104 54 1 13 114 72 175 6.47e-21 85.5
MsG0380016838.01.T01 MTR_3g073170 40.777 103 60 1 19 121 74 175 2.31e-20 84.0
MsG0380016838.01.T01 MTR_8g020760 41.818 110 63 1 8 117 64 172 3.83e-20 83.2
MsG0380016838.01.T01 MTR_5g031460 39.815 108 62 2 16 121 68 174 6.72e-20 82.8
MsG0380016838.01.T01 MTR_4g052770 39.806 103 61 1 22 123 74 176 5.63e-19 80.1
MsG0380016838.01.T01 MTR_4g052780 39.806 103 61 1 22 123 74 176 1.03e-18 79.3
MsG0380016838.01.T01 MTR_2g031300 49.367 79 38 1 13 89 34 112 1.44e-18 77.8
MsG0380016838.01.T01 MTR_2g019780 37.864 103 63 1 20 122 77 178 2.26e-18 78.6
MsG0380016838.01.T01 MTR_2g086640 38.596 114 65 4 14 123 62 174 9.33e-18 77.0
MsG0380016838.01.T01 MTR_2g086620 38.235 102 62 1 23 123 74 175 2.18e-17 75.9
MsG0380016838.01.T01 MTR_2g086630 37.255 102 63 1 23 123 74 175 6.16e-17 74.7
MsG0380016838.01.T01 MTR_8g020750 39.394 99 59 1 19 117 76 173 2.61e-16 73.2
MsG0380016838.01.T01 MTR_4g027415 44.444 72 39 1 18 89 72 142 3.09e-13 63.5
MsG0380016838.01.T01 MTR_3g019810 33.333 144 85 3 16 159 176 308 8.36e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016838.01.T01 AT5G61750 50.714 140 68 1 2 140 59 198 8.58e-44 144
MsG0380016838.01.T01 AT1G02335 47.541 122 63 1 3 123 63 184 2.36e-32 115
MsG0380016838.01.T01 AT1G09560 47.541 122 63 1 3 123 63 184 2.96e-31 112
MsG0380016838.01.T01 AT3G62020 47.321 112 58 1 13 123 44 155 7.99e-29 105
MsG0380016838.01.T01 AT3G62020 47.321 112 58 1 13 123 73 184 1.84e-28 105
MsG0380016838.01.T01 AT5G26700 46.610 118 63 0 6 123 64 181 6.42e-28 103
MsG0380016838.01.T01 AT1G72610 46.392 97 52 0 21 117 73 169 1.43e-27 102
MsG0380016838.01.T01 AT5G38960 44.538 119 62 2 3 117 67 185 1.81e-27 102
MsG0380016838.01.T01 AT3G10080 44.348 115 64 0 3 117 70 184 4.26e-27 102
MsG0380016838.01.T01 AT3G10080 44.348 115 64 0 3 117 141 255 1.71e-26 102
MsG0380016838.01.T01 AT3G05930 50.000 104 52 0 13 116 74 177 2.49e-26 99.8
MsG0380016838.01.T01 AT5G38910 40.559 143 81 2 12 152 75 215 8.08e-26 98.6
MsG0380016838.01.T01 AT5G38930 46.018 113 59 1 13 123 78 190 3.20e-25 97.1
MsG0380016838.01.T01 AT5G38940 46.729 107 55 1 13 117 78 184 4.75e-25 96.3
MsG0380016838.01.T01 AT5G38940 46.729 107 55 1 13 117 102 208 6.72e-25 96.7
MsG0380016838.01.T01 AT1G10460 41.985 131 68 3 3 125 61 191 9.37e-25 95.5
MsG0380016838.01.T01 AT5G20630 39.655 116 63 1 9 117 57 172 1.22e-23 92.4
MsG0380016838.01.T01 AT4G14630 34.839 155 95 3 2 152 66 218 4.95e-23 91.3
MsG0380016838.01.T01 AT3G04200 44.860 107 56 1 13 116 78 184 6.56e-23 90.9
MsG0380016838.01.T01 AT1G18970 42.500 120 66 1 7 123 68 187 1.39e-22 90.1
MsG0380016838.01.T01 AT3G05950 43.750 112 60 1 8 116 72 183 2.24e-22 89.7
MsG0380016838.01.T01 AT1G18980 43.636 110 59 1 14 123 81 187 2.29e-22 89.4
MsG0380016838.01.T01 AT3G04190 43.925 107 57 1 13 116 77 183 5.32e-22 88.6
MsG0380016838.01.T01 AT5G39110 35.220 159 87 4 3 152 65 216 1.15e-21 87.4
MsG0380016838.01.T01 AT5G39150 35.220 159 87 4 3 152 64 215 3.78e-21 86.3
MsG0380016838.01.T01 AT5G39120 35.220 159 87 4 3 152 64 215 3.94e-21 86.3
MsG0380016838.01.T01 AT5G39180 35.220 159 87 4 3 152 64 215 4.33e-21 86.3
MsG0380016838.01.T01 AT3G04180 41.964 112 62 1 8 116 72 183 6.13e-21 85.9
MsG0380016838.01.T01 AT3G04150 43.519 108 58 1 13 117 77 184 1.40e-20 84.7
MsG0380016838.01.T01 AT3G04170 38.514 148 76 3 19 151 80 227 1.50e-20 84.7
MsG0380016838.01.T01 AT3G04150 43.519 108 58 1 13 117 90 197 1.60e-20 85.1
MsG0380016838.01.T01 AT5G39160 41.667 108 60 1 13 117 53 160 2.22e-20 83.6
MsG0380016838.01.T01 AT5G39190 41.667 108 60 1 13 117 53 160 2.22e-20 83.6
MsG0380016838.01.T01 AT5G39130 38.760 129 71 2 13 133 75 203 2.27e-20 84.3
MsG0380016838.01.T01 AT5G39160 41.667 108 60 1 13 117 71 178 4.18e-20 83.6
MsG0380016838.01.T01 AT5G39160 37.984 129 72 2 13 133 75 203 4.46e-20 83.6
MsG0380016838.01.T01 AT5G39190 37.984 129 72 2 13 133 75 203 4.70e-20 83.6
MsG0380016838.01.T01 AT1G74820 39.000 100 61 0 10 109 81 180 1.76e-14 68.6

Find 37 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATCCCAACCCTGGATTT+TGG 0.211780 3:+88471733 None:intergenic
CACTAGCTCTTGGATGATTA+TGG 0.284341 3:+88471928 None:intergenic
GCAGGTTCAATAGATGATTC+TGG 0.356320 3:-88472053 MsG0380016838.01.T01:CDS
TAATGTTAATGATTTGAGTT+TGG 0.383346 3:-88471628 MsG0380016838.01.T01:CDS
CTTTATCTTCTCAACTGATT+CGG 0.407952 3:+88471686 None:intergenic
CTTTGTTGAGGAAGGTGTAT+TGG 0.411985 3:-88471898 MsG0380016838.01.T01:CDS
AAGAAATGGAACAAGCCCTT+AGG 0.421816 3:+88471804 None:intergenic
ACCTGCTTTGCTTAATTCCA+TGG 0.423219 3:+88472070 None:intergenic
AACACTCTTGGTATCTCAAT+TGG 0.444841 3:-88471987 MsG0380016838.01.T01:CDS
AGAACAGACATTGAAGTTGA+TGG 0.458478 3:-88471963 MsG0380016838.01.T01:CDS
CACCACGATTTAGAAAGAAA+TGG 0.459409 3:+88471790 None:intergenic
AAGATCATCTCACTAGCTCT+TGG 0.463818 3:+88471918 None:intergenic
TTCCATTTCTTTCTAAATCG+TGG 0.465993 3:-88471792 MsG0380016838.01.T01:CDS
AATAGCCAAAATCCAGGGTT+GGG 0.473458 3:-88471738 MsG0380016838.01.T01:CDS
AATCAGTTGAGAAGATAAAG+AGG 0.476922 3:-88471683 MsG0380016838.01.T01:CDS
AGAAATGGAACAAGCCCTTA+GGG 0.481120 3:+88471805 None:intergenic
GAGGAAGGTGTATTGGAAGC+TGG 0.485772 3:-88471891 MsG0380016838.01.T01:CDS
TTCCCTGGCCTCAACACTCT+TGG 0.487043 3:-88471999 MsG0380016838.01.T01:CDS
GTGTATAATAGCCAAAATCC+AGG 0.499696 3:-88471744 MsG0380016838.01.T01:CDS
TACCAAGAGTGTTGAGGCCA+GGG 0.502060 3:+88471997 None:intergenic
ATACCAAGAGTGTTGAGGCC+AGG 0.514315 3:+88471996 None:intergenic
TAATAGCCAAAATCCAGGGT+TGG 0.523741 3:-88471739 MsG0380016838.01.T01:CDS
GAACAGACATTGAAGTTGAT+GGG 0.524364 3:-88471962 MsG0380016838.01.T01:CDS
ACCATGGAATTAAGCAAAGC+AGG 0.525867 3:-88472071 MsG0380016838.01.T01:CDS
TCAACTGATTCGGAAGAAGA+AGG 0.533585 3:+88471696 None:intergenic
TTTCAGAAGTCTTTGAAGGA+AGG 0.534730 3:-88471843 MsG0380016838.01.T01:CDS
GTCACAGCTTCTAAGTTCCC+TGG 0.546660 3:-88472014 MsG0380016838.01.T01:CDS
TGATTTGAGTTTGGCTGTGT+CGG 0.549396 3:-88471619 MsG0380016838.01.T01:CDS
GCATTAAGAGATCCCAACCC+TGG 0.564662 3:+88471726 None:intergenic
ATTTCTTGATACACAAAACA+AGG 0.570126 3:-88471868 MsG0380016838.01.T01:CDS
TGATGTGTTTGTGATCCCTA+AGG 0.571996 3:-88471820 MsG0380016838.01.T01:CDS
TAGTGAGATGATCTTTGTTG+AGG 0.576125 3:-88471910 MsG0380016838.01.T01:CDS
GATGTGTTTGTGATCCCTAA+GGG 0.586453 3:-88471819 MsG0380016838.01.T01:CDS
GAGATGATCTTTGTTGAGGA+AGG 0.605865 3:-88471906 MsG0380016838.01.T01:CDS
TGTATAATAGCCAAAATCCA+GGG 0.650796 3:-88471743 MsG0380016838.01.T01:CDS
TTGAGATACCAAGAGTGTTG+AGG 0.653794 3:+88471991 None:intergenic
ACTGATTCGGAAGAAGAAGG+TGG 0.681202 3:+88471699 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGTTAATGATTTGAGTT+TGG - Chr3:88472042-88472061 MsG0380016838.01.T01:CDS 20.0%
! ATTTCTTGATACACAAAACA+AGG - Chr3:88471802-88471821 MsG0380016838.01.T01:CDS 25.0%
AATCAGTTGAGAAGATAAAG+AGG - Chr3:88471987-88472006 MsG0380016838.01.T01:CDS 30.0%
CTTTATCTTCTCAACTGATT+CGG + Chr3:88471987-88472006 None:intergenic 30.0%
TGTATAATAGCCAAAATCCA+GGG - Chr3:88471927-88471946 MsG0380016838.01.T01:CDS 30.0%
TTCCATTTCTTTCTAAATCG+TGG - Chr3:88471878-88471897 MsG0380016838.01.T01:CDS 30.0%
AACACTCTTGGTATCTCAAT+TGG - Chr3:88471683-88471702 MsG0380016838.01.T01:CDS 35.0%
AGAACAGACATTGAAGTTGA+TGG - Chr3:88471707-88471726 MsG0380016838.01.T01:CDS 35.0%
CACCACGATTTAGAAAGAAA+TGG + Chr3:88471883-88471902 None:intergenic 35.0%
GTGTATAATAGCCAAAATCC+AGG - Chr3:88471926-88471945 MsG0380016838.01.T01:CDS 35.0%
TAGTGAGATGATCTTTGTTG+AGG - Chr3:88471760-88471779 MsG0380016838.01.T01:CDS 35.0%
! GAACAGACATTGAAGTTGAT+GGG - Chr3:88471708-88471727 MsG0380016838.01.T01:CDS 35.0%
! TTTCAGAAGTCTTTGAAGGA+AGG - Chr3:88471827-88471846 MsG0380016838.01.T01:CDS 35.0%
!! GGTTTTTCAGAAGTCTTTGA+AGG - Chr3:88471823-88471842 MsG0380016838.01.T01:CDS 35.0%
AAGAAATGGAACAAGCCCTT+AGG + Chr3:88471869-88471888 None:intergenic 40.0%
AATAGCCAAAATCCAGGGTT+GGG - Chr3:88471932-88471951 MsG0380016838.01.T01:CDS 40.0%
AGAAATGGAACAAGCCCTTA+GGG + Chr3:88471868-88471887 None:intergenic 40.0%
GAGATGATCTTTGTTGAGGA+AGG - Chr3:88471764-88471783 MsG0380016838.01.T01:CDS 40.0%
GATGTGTTTGTGATCCCTAA+GGG - Chr3:88471851-88471870 MsG0380016838.01.T01:CDS 40.0%
GCAGGTTCAATAGATGATTC+TGG - Chr3:88471617-88471636 MsG0380016838.01.T01:CDS 40.0%
TAATAGCCAAAATCCAGGGT+TGG - Chr3:88471931-88471950 MsG0380016838.01.T01:CDS 40.0%
TGATGTGTTTGTGATCCCTA+AGG - Chr3:88471850-88471869 MsG0380016838.01.T01:CDS 40.0%
! AAGATCATCTCACTAGCTCT+TGG + Chr3:88471755-88471774 None:intergenic 40.0%
! CACTAGCTCTTGGATGATTA+TGG + Chr3:88471745-88471764 None:intergenic 40.0%
! CTTTGTTGAGGAAGGTGTAT+TGG - Chr3:88471772-88471791 MsG0380016838.01.T01:CDS 40.0%
! TGATTTGAGTTTGGCTGTGT+CGG - Chr3:88472051-88472070 MsG0380016838.01.T01:CDS 40.0%
!! TCAACTGATTCGGAAGAAGA+AGG + Chr3:88471977-88471996 None:intergenic 40.0%
!! TTGAGATACCAAGAGTGTTG+AGG + Chr3:88471682-88471701 None:intergenic 40.0%
!! ACTGATTCGGAAGAAGAAGG+TGG + Chr3:88471974-88471993 None:intergenic 45.0%
GCATTAAGAGATCCCAACCC+TGG + Chr3:88471947-88471966 None:intergenic 50.0%
GTCACAGCTTCTAAGTTCCC+TGG - Chr3:88471656-88471675 MsG0380016838.01.T01:CDS 50.0%
! GAGATCCCAACCCTGGATTT+TGG + Chr3:88471940-88471959 None:intergenic 50.0%
! GAGGAAGGTGTATTGGAAGC+TGG - Chr3:88471779-88471798 MsG0380016838.01.T01:CDS 50.0%
!! ATACCAAGAGTGTTGAGGCC+AGG + Chr3:88471677-88471696 None:intergenic 50.0%
!! TACCAAGAGTGTTGAGGCCA+GGG + Chr3:88471676-88471695 None:intergenic 50.0%
TTCCCTGGCCTCAACACTCT+TGG - Chr3:88471671-88471690 MsG0380016838.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 88471602 88472090 88471602 ID=MsG0380016838.01;Name=MsG0380016838.01
Chr3 mRNA 88471602 88472090 88471602 ID=MsG0380016838.01.T01;Parent=MsG0380016838.01;Name=MsG0380016838.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|162
Chr3 exon 88471602 88472090 88471602 ID=MsG0380016838.01.T01:exon:24990;Parent=MsG0380016838.01.T01
Chr3 CDS 88471602 88472090 88471602 ID=MsG0380016838.01.T01:cds;Parent=MsG0380016838.01.T01
Gene Sequence

>MsG0380016838.01.T01

ATGGAATTAAGCAAAGCAGGTTCAATAGATGATTCTGGTTCATCAATCAACATCGTCACAGCTTCTAAGTTCCCTGGCCTCAACACTCTTGGTATCTCAATTGGCAGAACAGACATTGAAGTTGATGGGATTGTGAATCTCCATAATCATCCAAGAGCTAGTGAGATGATCTTTGTTGAGGAAGGTGTATTGGAAGCTGGATTTCTTGATACACAAAACAAGGTTTTTCAGAAGTCTTTGAAGGAAGGTGATGTGTTTGTGATCCCTAAGGGCTTGTTCCATTTCTTTCTAAATCGTGGTGTAGAAGTTGCAACTGTGTTGTCTGTGTATAATAGCCAAAATCCAGGGTTGGGATCTCTTAATGCTACACCACCTTCTTCTTCCGAATCAGTTGAGAAGATAAAGAGGAAGCTTATATCACTTTATGAGTTAGAACTTGATAATGTTAATGATTTGAGTTTGGCTGTGTCGGAGATTATTTACAGTTAG

Protein sequence

>MsG0380016838.01.T01

MELSKAGSIDDSGSSINIVTASKFPGLNTLGISIGRTDIEVDGIVNLHNHPRASEMIFVEEGVLEAGFLDTQNKVFQKSLKEGDVFVIPKGLFHFFLNRGVEVATVLSVYNSQNPGLGSLNATPPSSSESVEKIKRKLISLYELELDNVNDLSLAVSEIIYS*