Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017179.01.T01 | XP_013461729.1 | 94.1 | 339 | 15 | 1 | 1 | 334 | 1 | 339 | 0 | 652 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017179.01.T01 | Q9FMH5 | 52.339 | 342 | 154 | 1 | 3 | 335 | 12 | 353 | 2.17E-123 | 361 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017179.01.T01 | A0A072V166 | 94.100 | 339 | 15 | 1 | 1 | 334 | 1 | 339 | 0.0 | 652 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380017179.01 | MsG0580025186.01 | 0.808351 | 3.231930e-50 | 1.843814e-47 |
| MsG0380017179.01 | MsG0580028923.01 | 0.800370 | 1.488027e-48 | 6.916910e-46 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017179.01.T01 | MTR_3g102510 | 94.100 | 339 | 15 | 1 | 1 | 334 | 1 | 339 | 0.0 | 652 |
| MsG0380017179.01.T01 | MTR_3g102520 | 78.371 | 356 | 53 | 3 | 1 | 332 | 1 | 356 | 0.0 | 564 |
| MsG0380017179.01.T01 | MTR_3g102530 | 67.697 | 356 | 88 | 5 | 1 | 334 | 1 | 351 | 1.92e-166 | 468 |
| MsG0380017179.01.T01 | MTR_3g102560 | 54.896 | 337 | 133 | 6 | 1 | 332 | 2 | 324 | 7.49e-113 | 330 |
| MsG0380017179.01.T01 | MTR_1g018680 | 58.545 | 275 | 112 | 1 | 60 | 332 | 82 | 356 | 1.60e-112 | 331 |
| MsG0380017179.01.T01 | MTR_3g102540 | 55.273 | 275 | 113 | 4 | 61 | 332 | 1 | 268 | 7.45e-98 | 291 |
| MsG0380017179.01.T01 | MTR_3g088415 | 46.886 | 273 | 145 | 0 | 60 | 332 | 231 | 503 | 1.17e-82 | 259 |
| MsG0380017179.01.T01 | MTR_1g011470 | 48.649 | 259 | 132 | 1 | 69 | 326 | 161 | 419 | 5.44e-82 | 255 |
| MsG0380017179.01.T01 | MTR_3g088405 | 46.947 | 262 | 137 | 1 | 69 | 328 | 243 | 504 | 5.92e-81 | 255 |
| MsG0380017179.01.T01 | MTR_3g107970 | 45.865 | 266 | 144 | 0 | 60 | 325 | 204 | 469 | 6.25e-80 | 251 |
| MsG0380017179.01.T01 | MTR_2g102550 | 42.647 | 272 | 151 | 1 | 58 | 324 | 151 | 422 | 4.07e-72 | 230 |
| MsG0380017179.01.T01 | MTR_2g102520 | 44.944 | 267 | 145 | 1 | 60 | 324 | 206 | 472 | 4.54e-71 | 229 |
| MsG0380017179.01.T01 | MTR_3g088415 | 49.282 | 209 | 106 | 0 | 60 | 268 | 231 | 439 | 5.69e-67 | 217 |
| MsG0380017179.01.T01 | MTR_3g088415 | 52.258 | 155 | 74 | 0 | 60 | 214 | 231 | 385 | 2.33e-50 | 172 |
| MsG0380017179.01.T01 | MTR_8g074000 | 34.921 | 252 | 155 | 4 | 69 | 319 | 176 | 419 | 3.02e-45 | 160 |
| MsG0380017179.01.T01 | MTR_5g023790 | 35.317 | 252 | 154 | 5 | 69 | 319 | 172 | 415 | 1.13e-42 | 153 |
| MsG0380017179.01.T01 | MTR_7g089080 | 35.135 | 259 | 156 | 5 | 69 | 322 | 199 | 450 | 2.98e-42 | 157 |
| MsG0380017179.01.T01 | MTR_5g088980 | 34.979 | 243 | 150 | 5 | 69 | 310 | 165 | 400 | 3.32e-42 | 151 |
| MsG0380017179.01.T01 | MTR_5g023790 | 38.220 | 191 | 112 | 2 | 69 | 259 | 172 | 356 | 9.51e-38 | 139 |
| MsG0380017179.01.T01 | MTR_5g023790 | 40.397 | 151 | 89 | 1 | 69 | 219 | 172 | 321 | 8.57e-31 | 119 |
| MsG0380017179.01.T01 | MTR_3g060410 | 32.161 | 199 | 126 | 5 | 98 | 291 | 131 | 325 | 1.18e-21 | 94.7 |
| MsG0380017179.01.T01 | MTR_3g060770 | 29.362 | 235 | 139 | 8 | 102 | 320 | 60 | 283 | 3.13e-20 | 89.4 |
| MsG0380017179.01.T01 | MTR_3g060820 | 31.313 | 198 | 121 | 6 | 102 | 291 | 50 | 240 | 4.42e-20 | 89.0 |
| MsG0380017179.01.T01 | MTR_3g061680 | 31.313 | 198 | 121 | 6 | 102 | 291 | 50 | 240 | 1.20e-19 | 87.8 |
| MsG0380017179.01.T01 | MTR_3g060400 | 30.542 | 203 | 124 | 7 | 98 | 291 | 52 | 246 | 2.24e-18 | 84.3 |
| MsG0380017179.01.T01 | MTR_3g460990 | 30.542 | 203 | 124 | 7 | 98 | 291 | 52 | 246 | 2.24e-18 | 84.3 |
| MsG0380017179.01.T01 | MTR_5g044040 | 41.333 | 75 | 44 | 0 | 124 | 198 | 7 | 81 | 6.04e-14 | 68.2 |
| MsG0380017179.01.T01 | MTR_6g045270 | 29.902 | 204 | 122 | 8 | 98 | 294 | 70 | 259 | 1.28e-13 | 70.9 |
| MsG0380017179.01.T01 | MTR_8g063120 | 33.577 | 137 | 84 | 4 | 81 | 211 | 71 | 206 | 9.46e-13 | 68.2 |
| MsG0380017179.01.T01 | MTR_8g063120 | 33.577 | 137 | 84 | 4 | 81 | 211 | 71 | 206 | 2.10e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017179.01.T01 | AT5G43080 | 52.339 | 342 | 154 | 1 | 3 | 335 | 12 | 353 | 2.21e-124 | 361 |
| MsG0380017179.01.T01 | AT5G43080 | 51.462 | 342 | 152 | 2 | 3 | 335 | 12 | 348 | 8.13e-121 | 352 |
| MsG0380017179.01.T01 | AT1G47230 | 47.740 | 354 | 163 | 4 | 3 | 335 | 11 | 363 | 2.07e-109 | 323 |
| MsG0380017179.01.T01 | AT1G47230 | 47.606 | 355 | 163 | 5 | 3 | 335 | 11 | 364 | 1.41e-107 | 319 |
| MsG0380017179.01.T01 | AT1G47210 | 46.667 | 360 | 165 | 5 | 3 | 335 | 11 | 370 | 6.76e-106 | 315 |
| MsG0380017179.01.T01 | AT1G47220 | 54.647 | 269 | 120 | 1 | 69 | 335 | 56 | 324 | 2.33e-98 | 294 |
| MsG0380017179.01.T01 | AT1G44110 | 49.242 | 264 | 134 | 0 | 69 | 332 | 194 | 457 | 2.92e-82 | 257 |
| MsG0380017179.01.T01 | AT5G11300 | 46.377 | 276 | 143 | 1 | 61 | 331 | 159 | 434 | 1.98e-80 | 251 |
| MsG0380017179.01.T01 | AT1G80370 | 46.038 | 265 | 142 | 1 | 69 | 333 | 197 | 460 | 5.21e-77 | 243 |
| MsG0380017179.01.T01 | AT5G25380 | 44.487 | 263 | 146 | 0 | 69 | 331 | 173 | 435 | 8.38e-76 | 239 |
| MsG0380017179.01.T01 | AT5G25380 | 44.487 | 263 | 146 | 0 | 69 | 331 | 173 | 435 | 8.38e-76 | 239 |
| MsG0380017179.01.T01 | AT5G25380 | 44.487 | 263 | 146 | 0 | 69 | 331 | 179 | 441 | 1.22e-75 | 239 |
| MsG0380017179.01.T01 | AT1G15570 | 45.312 | 256 | 140 | 0 | 69 | 324 | 188 | 443 | 6.20e-74 | 235 |
| MsG0380017179.01.T01 | AT1G77390 | 41.241 | 274 | 161 | 0 | 60 | 333 | 169 | 442 | 5.63e-72 | 230 |
| MsG0380017179.01.T01 | AT5G25380 | 44.715 | 246 | 136 | 0 | 69 | 314 | 173 | 418 | 1.93e-70 | 225 |
| MsG0380017179.01.T01 | AT5G11300 | 47.639 | 233 | 108 | 2 | 61 | 279 | 159 | 391 | 5.04e-67 | 216 |
| MsG0380017179.01.T01 | AT3G11520 | 36.965 | 257 | 151 | 6 | 69 | 324 | 159 | 405 | 3.11e-43 | 154 |
| MsG0380017179.01.T01 | AT4G37490 | 38.839 | 224 | 128 | 5 | 69 | 291 | 165 | 380 | 4.82e-42 | 151 |
| MsG0380017179.01.T01 | AT5G06150 | 35.317 | 252 | 153 | 5 | 69 | 319 | 182 | 424 | 1.01e-41 | 150 |
| MsG0380017179.01.T01 | AT3G11520 | 38.667 | 225 | 128 | 5 | 69 | 292 | 159 | 374 | 2.94e-41 | 148 |
| MsG0380017179.01.T01 | AT1G20610 | 34.524 | 252 | 156 | 4 | 69 | 319 | 177 | 420 | 1.57e-40 | 147 |
| MsG0380017179.01.T01 | AT1G76310 | 35.433 | 254 | 156 | 4 | 69 | 321 | 177 | 423 | 2.40e-40 | 147 |
| MsG0380017179.01.T01 | AT2G17620 | 36.604 | 265 | 161 | 3 | 55 | 319 | 157 | 414 | 7.70e-40 | 145 |
| MsG0380017179.01.T01 | AT2G26760 | 37.891 | 256 | 151 | 5 | 69 | 323 | 130 | 378 | 1.31e-39 | 144 |
| MsG0380017179.01.T01 | AT4G35620 | 36.255 | 251 | 153 | 3 | 69 | 319 | 172 | 415 | 5.25e-39 | 143 |
| MsG0380017179.01.T01 | AT1G16330 | 31.276 | 243 | 158 | 4 | 69 | 310 | 395 | 629 | 7.35e-36 | 137 |
| MsG0380017179.01.T01 | AT1G76310 | 38.265 | 196 | 114 | 3 | 69 | 263 | 177 | 366 | 1.57e-35 | 133 |
| MsG0380017179.01.T01 | AT4G35620 | 40.838 | 191 | 107 | 2 | 69 | 259 | 172 | 356 | 6.83e-35 | 131 |
| MsG0380017179.01.T01 | AT1G76310 | 31.915 | 282 | 156 | 5 | 69 | 321 | 177 | 451 | 1.27e-34 | 132 |
| MsG0380017179.01.T01 | AT1G47210 | 41.758 | 182 | 79 | 5 | 3 | 157 | 11 | 192 | 1.63e-31 | 117 |
| MsG0380017179.01.T01 | AT5G06150 | 41.958 | 143 | 81 | 2 | 69 | 211 | 182 | 322 | 2.71e-29 | 115 |
| MsG0380017179.01.T01 | AT1G76310 | 40.523 | 153 | 89 | 2 | 69 | 220 | 177 | 328 | 3.01e-28 | 112 |
| MsG0380017179.01.T01 | AT1G20590 | 37.107 | 159 | 92 | 3 | 131 | 288 | 66 | 217 | 5.04e-24 | 99.4 |
| MsG0380017179.01.T01 | AT1G14750 | 31.633 | 196 | 113 | 6 | 76 | 258 | 185 | 372 | 9.48e-16 | 78.2 |
| MsG0380017179.01.T01 | AT1G14750 | 31.633 | 196 | 113 | 6 | 76 | 258 | 353 | 540 | 2.68e-15 | 77.4 |
| MsG0380017179.01.T01 | AT1G14750 | 31.633 | 196 | 113 | 6 | 76 | 258 | 353 | 540 | 3.03e-15 | 77.0 |
| MsG0380017179.01.T01 | AT1G14750 | 32.143 | 196 | 111 | 7 | 76 | 258 | 185 | 371 | 1.03e-14 | 75.1 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 272 | 392 | 1.63e-12 | 68.6 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 272 | 392 | 1.63e-12 | 68.6 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 272 | 392 | 1.63e-12 | 68.6 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 272 | 392 | 1.63e-12 | 68.6 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 258 | 378 | 1.65e-12 | 68.6 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 272 | 392 | 1.84e-12 | 68.6 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 272 | 392 | 1.99e-12 | 68.2 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 269 | 389 | 2.17e-12 | 68.2 |
| MsG0380017179.01.T01 | AT1G34460 | 30.769 | 143 | 77 | 3 | 69 | 211 | 269 | 389 | 2.17e-12 | 68.2 |
Find 76 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTCCCATTTCAAAATTT+AGG | 0.145584 | 3:+93041490 | None:intergenic |
| ACCTTTCCTTAATTCCAATT+TGG | 0.169676 | 3:-93042108 | MsG0380017179.01.T01:CDS |
| CAAAAGACAGAGAGTTGTTT+TGG | 0.206240 | 3:-93042216 | MsG0380017179.01.T01:CDS |
| ACAGAGAGATGAAGGGTTTC+TGG | 0.207212 | 3:+93041921 | None:intergenic |
| CAAAACAACTCTCTGTCTTT+TGG | 0.223684 | 3:+93042217 | None:intergenic |
| GCTTATCCAAATTGGAATTA+AGG | 0.235094 | 3:+93042102 | None:intergenic |
| TTTAGAAATGTGATGACATT+AGG | 0.241622 | 3:+93041468 | None:intergenic |
| GTTTCCCATTTCAAAATTTA+GGG | 0.248370 | 3:+93041491 | None:intergenic |
| TACATAAGGCTTATCCAAAT+TGG | 0.252861 | 3:+93042094 | None:intergenic |
| ACTTCTATAAAGTAATAATT+TGG | 0.267667 | 3:+93040717 | None:intergenic |
| AAGACCAATGGTTGGTTATA+TGG | 0.298772 | 3:-93042030 | MsG0380017179.01.T01:CDS |
| TGAGATTGATTTGTTGAGAT+TGG | 0.300594 | 3:+93042136 | None:intergenic |
| TGTACATTGGAGTTTCTGTT+TGG | 0.309368 | 3:+93042166 | None:intergenic |
| CAGATATTCAAACTGCAAAT+TGG | 0.319844 | 3:+93041214 | None:intergenic |
| TTAGAAATGTGATGACATTA+GGG | 0.330376 | 3:+93041469 | None:intergenic |
| TTCATCCCTTTGTGAATGCT+TGG | 0.338640 | 3:-93040977 | MsG0380017179.01.T01:CDS |
| CATAGGTGTTATCAGTGATT+TGG | 0.355291 | 3:+93041673 | None:intergenic |
| GACATGTGGTTGTTGTTGAT+TGG | 0.377903 | 3:+93042247 | None:intergenic |
| TGAGAGGGACATTGGTTGAT+TGG | 0.379637 | 3:-93041977 | MsG0380017179.01.T01:CDS |
| TGGCAGAAATCTATAGCCTT+TGG | 0.389420 | 3:+93041693 | None:intergenic |
| TCATCCCTTTGTGAATGCTT+GGG | 0.393250 | 3:-93040976 | MsG0380017179.01.T01:CDS |
| GGGTTACAAGTCGGCTGAAT+TGG | 0.394636 | 3:-93040956 | MsG0380017179.01.T01:CDS |
| AAGTAATAATTTGGTATCTC+TGG | 0.395913 | 3:+93040726 | None:intergenic |
| TTTCTCCTTAACAGCTTTGA+AGG | 0.397121 | 3:+93040882 | None:intergenic |
| GTATCTCTGGCGGGGAAGGC+AGG | 0.399107 | 3:+93040739 | None:intergenic |
| GAGATTGATTTGTTGAGATT+GGG | 0.413711 | 3:+93042137 | None:intergenic |
| GATGAAGGGTTTCTGGAAGA+AGG | 0.417673 | 3:+93041928 | None:intergenic |
| GTACATTGGAGTTTCTGTTT+GGG | 0.433143 | 3:+93042167 | None:intergenic |
| TATTACCTCGTCAAGATCAT+AGG | 0.437323 | 3:+93041656 | None:intergenic |
| TTCTTGCTAGATTTATTCTC+CGG | 0.441267 | 3:-93041109 | MsG0380017179.01.T01:CDS |
| TTATACATTCATAGTCCAAC+AGG | 0.453800 | 3:+93041168 | None:intergenic |
| TGAAGCTTAGATCTAATGAT+AGG | 0.454505 | 3:+93041867 | None:intergenic |
| AGATCTAAGCTTCAGTTGCT+TGG | 0.464129 | 3:-93041858 | MsG0380017179.01.T01:CDS |
| AAGATTCTTGCCTTCTGTTG+TGG | 0.472652 | 3:-93041147 | MsG0380017179.01.T01:CDS |
| AGCATCCTTCAAAGCTGTTA+AGG | 0.479438 | 3:-93040887 | MsG0380017179.01.T01:CDS |
| ATTGGTTGATTGGTTAGTTG+AGG | 0.479448 | 3:-93041967 | MsG0380017179.01.T01:CDS |
| AATAATTTGGTATCTCTGGC+GGG | 0.480876 | 3:+93040730 | None:intergenic |
| TGTATGAAACAGAGAGATGA+AGG | 0.484873 | 3:+93041913 | None:intergenic |
| TCGAATATGTCGGATACATA+AGG | 0.485282 | 3:+93042080 | None:intergenic |
| ATGACTTGTATTTATCAAGA+AGG | 0.491239 | 3:-93040912 | MsG0380017179.01.T01:CDS |
| CCGGCCTGGAGTACATCCTT+CGG | 0.496877 | 3:-93041090 | MsG0380017179.01.T01:intron |
| TTTGGTATCTCTGGCGGGGA+AGG | 0.505867 | 3:+93040735 | None:intergenic |
| CAGAAATCTATAGCCTTTGG+TGG | 0.507750 | 3:+93041696 | None:intergenic |
| AATTGGAATTAAGGAAAGGT+AGG | 0.510771 | 3:+93042111 | None:intergenic |
| GCTAGATTTATTCTCCGGCC+TGG | 0.514323 | 3:-93041104 | MsG0380017179.01.T01:CDS |
| GGAGAAATACAAGCAACAAA+AGG | 0.518364 | 3:-93040866 | MsG0380017179.01.T01:intron |
| GCTTAGATCTAATGATAGGT+TGG | 0.520901 | 3:+93041871 | None:intergenic |
| TGACTTGTATTTATCAAGAA+GGG | 0.521205 | 3:-93040911 | MsG0380017179.01.T01:CDS |
| GATTGCTTCTGAGAATCAAA+AGG | 0.529405 | 3:-93041329 | MsG0380017179.01.T01:intron |
| ATCAAACATGAGAGGGACAT+TGG | 0.531177 | 3:-93041985 | MsG0380017179.01.T01:CDS |
| AGAAATCTATAGCCTTTGGT+GGG | 0.532149 | 3:+93041697 | None:intergenic |
| TCTGGAACAGTTCAAATATG+TGG | 0.532832 | 3:-93040766 | MsG0380017179.01.T01:intron |
| TCCAAATTGGAATTAAGGAA+AGG | 0.539850 | 3:+93042107 | None:intergenic |
| ACAGAAGCAGCCACAACAGA+AGG | 0.558857 | 3:+93041137 | None:intergenic |
| ATTGGAATTAAGGAAAGGTA+GGG | 0.562882 | 3:+93042112 | None:intergenic |
| TAATAATTTGGTATCTCTGG+CGG | 0.563787 | 3:+93040729 | None:intergenic |
| ATTCGATTATCTTCGAACAA+TGG | 0.568440 | 3:-93042063 | MsG0380017179.01.T01:CDS |
| TTTACCGAAGGATGTACTCC+AGG | 0.578118 | 3:+93041086 | None:intergenic |
| GTAACCCAAGCATTCACAAA+GGG | 0.587375 | 3:+93040972 | None:intergenic |
| CCGAAGGATGTACTCCAGGC+CGG | 0.588796 | 3:+93041090 | None:intergenic |
| TCAACAACAACCACATGTCA+TGG | 0.598129 | 3:-93042243 | MsG0380017179.01.T01:CDS |
| TGAATGCTTGGGTTACAAGT+CGG | 0.600751 | 3:-93040965 | MsG0380017179.01.T01:CDS |
| ATCTTACGATGCAATGAGCA+TGG | 0.601202 | 3:+93041827 | None:intergenic |
| ATAATTTGGTATCTCTGGCG+GGG | 0.610149 | 3:+93040731 | None:intergenic |
| TGAAGAAATAACCCCACCAA+AGG | 0.619552 | 3:-93041709 | MsG0380017179.01.T01:CDS |
| TTCTCCATATAACCAACCAT+TGG | 0.621997 | 3:+93042026 | None:intergenic |
| ATATCTGTGCAACTATCTTG+CGG | 0.626935 | 3:-93041198 | MsG0380017179.01.T01:CDS |
| GCATTACATCAAACATGAGA+GGG | 0.627034 | 3:-93041992 | MsG0380017179.01.T01:CDS |
| GGCATTACATCAAACATGAG+AGG | 0.629142 | 3:-93041993 | MsG0380017179.01.T01:CDS |
| GAAATCTATAGCCTTTGGTG+GGG | 0.630025 | 3:+93041698 | None:intergenic |
| TCTTGCGGATATAAGCCTGT+TGG | 0.638118 | 3:-93041183 | MsG0380017179.01.T01:CDS |
| TCGAAGATAATCGAATATGT+CGG | 0.658989 | 3:+93042070 | None:intergenic |
| TATATGGAGAATGTTCAAAG+AGG | 0.675074 | 3:-93042014 | MsG0380017179.01.T01:CDS |
| GTATGAAACAGAGAGATGAA+GGG | 0.679453 | 3:+93041914 | None:intergenic |
| TGTAACCCAAGCATTCACAA+AGG | 0.709657 | 3:+93040971 | None:intergenic |
| TAACACCTATGATCTTGACG+AGG | 0.759007 | 3:-93041661 | MsG0380017179.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAAGAATAATATTTCA+AGG | - | Chr3:93041966-93041985 | MsG0380017179.01.T01:CDS | 10.0% |
| !!! | TAATATAGTATTCTTTTATT+TGG | - | Chr3:93041356-93041375 | MsG0380017179.01.T01:intron | 10.0% |
| !! | ACTTCTATAAAGTAATAATT+TGG | + | Chr3:93042280-93042299 | None:intergenic | 15.0% |
| !! | ATTGTAAAATCGAATATATA+AGG | - | Chr3:93041566-93041585 | MsG0380017179.01.T01:intron | 15.0% |
| !!! | TCGATTTTACAATAAAAAAA+AGG | + | Chr3:93041559-93041578 | None:intergenic | 15.0% |
| !! | CAATTAAATTCATTTCAACA+AGG | + | Chr3:93041592-93041611 | None:intergenic | 20.0% |
| !! | CTTGTTGAAATGAATTTAAT+TGG | - | Chr3:93041590-93041609 | MsG0380017179.01.T01:intron | 20.0% |
| !! | TTTAATTCTATTCAATATGC+AGG | - | Chr3:93041257-93041276 | MsG0380017179.01.T01:intron | 20.0% |
| !!! | TATCAAATTTTGTTCTTGAT+TGG | - | Chr3:93041384-93041403 | MsG0380017179.01.T01:intron | 20.0% |
| ! | AAGTAATAATTTGGTATCTC+TGG | + | Chr3:93042271-93042290 | None:intergenic | 25.0% |
| ! | GTTTCCCATTTCAAAATTTA+GGG | + | Chr3:93041506-93041525 | None:intergenic | 25.0% |
| ! | TAACATCAAATATTTACCGA+AGG | + | Chr3:93041923-93041942 | None:intergenic | 25.0% |
| ! | TTAGAAATGTGATGACATTA+GGG | + | Chr3:93041528-93041547 | None:intergenic | 25.0% |
| ! | TTTAGAAATGTGATGACATT+AGG | + | Chr3:93041529-93041548 | None:intergenic | 25.0% |
| !! | ATGACTTGTATTTATCAAGA+AGG | - | Chr3:93042082-93042101 | MsG0380017179.01.T01:CDS | 25.0% |
| !! | TGACTTGTATTTATCAAGAA+GGG | - | Chr3:93042083-93042102 | MsG0380017179.01.T01:CDS | 25.0% |
| !!! | AAGACCCTAAATTTTGAAAT+GGG | - | Chr3:93041499-93041518 | MsG0380017179.01.T01:CDS | 25.0% |
| !!! | TTGGGTTGATAATAAAAATC+AGG | + | Chr3:93040812-93040831 | None:intergenic | 25.0% |
| AATTGGAATTAAGGAAAGGT+AGG | + | Chr3:93040886-93040905 | None:intergenic | 30.0% | |
| ACCTTTCCTTAATTCCAATT+TGG | - | Chr3:93040886-93040905 | MsG0380017179.01.T01:CDS | 30.0% | |
| ATGTATGCTGTTGTTAATGT+AGG | - | Chr3:93041450-93041469 | MsG0380017179.01.T01:intron | 30.0% | |
| ATTCGATTATCTTCGAACAA+TGG | - | Chr3:93040931-93040950 | MsG0380017179.01.T01:CDS | 30.0% | |
| ATTGGAATTAAGGAAAGGTA+GGG | + | Chr3:93040885-93040904 | None:intergenic | 30.0% | |
| CAATTAGATCACACAATAAC+AGG | + | Chr3:93041733-93041752 | None:intergenic | 30.0% | |
| CAGATATTCAAACTGCAAAT+TGG | + | Chr3:93041783-93041802 | None:intergenic | 30.0% | |
| GCTTATCCAAATTGGAATTA+AGG | + | Chr3:93040895-93040914 | None:intergenic | 30.0% | |
| GGTTTCCCATTTCAAAATTT+AGG | + | Chr3:93041507-93041526 | None:intergenic | 30.0% | |
| TAATAATTTGGTATCTCTGG+CGG | + | Chr3:93042268-93042287 | None:intergenic | 30.0% | |
| TACATAAGGCTTATCCAAAT+TGG | + | Chr3:93040903-93040922 | None:intergenic | 30.0% | |
| TATATGGAGAATGTTCAAAG+AGG | - | Chr3:93040980-93040999 | MsG0380017179.01.T01:CDS | 30.0% | |
| TCCAAATTGGAATTAAGGAA+AGG | + | Chr3:93040890-93040909 | None:intergenic | 30.0% | |
| TCGAAGATAATCGAATATGT+CGG | + | Chr3:93040927-93040946 | None:intergenic | 30.0% | |
| TGAAGCTTAGATCTAATGAT+AGG | + | Chr3:93041130-93041149 | None:intergenic | 30.0% | |
| TTATACATTCATAGTCCAAC+AGG | + | Chr3:93041829-93041848 | None:intergenic | 30.0% | |
| TTCTTGCTAGATTTATTCTC+CGG | - | Chr3:93041885-93041904 | MsG0380017179.01.T01:CDS | 30.0% | |
| ! | AAAACAACTCTCTGTCTTTT+GGG | + | Chr3:93040779-93040798 | None:intergenic | 30.0% |
| !! | AAAAGACAGAGAGTTGTTTT+GGG | - | Chr3:93040779-93040798 | MsG0380017179.01.T01:intron | 30.0% |
| !! | ATTCATTTCAGGAGTTTTGT+TGG | - | Chr3:93041642-93041661 | MsG0380017179.01.T01:intron | 30.0% |
| !! | CAAGACCCTAAATTTTGAAA+TGG | - | Chr3:93041498-93041517 | MsG0380017179.01.T01:CDS | 30.0% |
| !! | GAGATTGATTTGTTGAGATT+GGG | + | Chr3:93040860-93040879 | None:intergenic | 30.0% |
| !! | TGAGATTGATTTGTTGAGAT+TGG | + | Chr3:93040861-93040880 | None:intergenic | 30.0% |
| !!! | AATGCTGTTTTATTACTAGC+TGG | - | Chr3:93041696-93041715 | MsG0380017179.01.T01:CDS | 30.0% |
| !!! | GATTGGGATTTTTTGTACAT+TGG | + | Chr3:93040844-93040863 | None:intergenic | 30.0% |
| !!! | GTTAATGTAGGTTTTGAAGA+TGG | - | Chr3:93041462-93041481 | MsG0380017179.01.T01:intron | 30.0% |
| !!! | TTCATTTCAGGAGTTTTGTT+GGG | - | Chr3:93041643-93041662 | MsG0380017179.01.T01:intron | 30.0% |
| AAAAAGAGAAGACCAATGGT+TGG | - | Chr3:93040956-93040975 | MsG0380017179.01.T01:CDS | 35.0% | |
| AATAATTTGGTATCTCTGGC+GGG | + | Chr3:93042267-93042286 | None:intergenic | 35.0% | |
| AGAAATCTATAGCCTTTGGT+GGG | + | Chr3:93041300-93041319 | None:intergenic | 35.0% | |
| ATATCTGTGCAACTATCTTG+CGG | - | Chr3:93041796-93041815 | MsG0380017179.01.T01:intron | 35.0% | |
| GCATTACATCAAACATGAGA+GGG | - | Chr3:93041002-93041021 | MsG0380017179.01.T01:intron | 35.0% | |
| GCTTAGATCTAATGATAGGT+TGG | + | Chr3:93041126-93041145 | None:intergenic | 35.0% | |
| GGAGAAATACAAGCAACAAA+AGG | - | Chr3:93042128-93042147 | MsG0380017179.01.T01:CDS | 35.0% | |
| GTACATTGGAGTTTCTGTTT+GGG | + | Chr3:93040830-93040849 | None:intergenic | 35.0% | |
| GTATGAAACAGAGAGATGAA+GGG | + | Chr3:93041083-93041102 | None:intergenic | 35.0% | |
| TATTACCTCGTCAAGATCAT+AGG | + | Chr3:93041341-93041360 | None:intergenic | 35.0% | |
| TCGAATATGTCGGATACATA+AGG | + | Chr3:93040917-93040936 | None:intergenic | 35.0% | |
| TGTACATTGGAGTTTCTGTT+TGG | + | Chr3:93040831-93040850 | None:intergenic | 35.0% | |
| TGTATGAAACAGAGAGATGA+AGG | + | Chr3:93041084-93041103 | None:intergenic | 35.0% | |
| TTCTCCATATAACCAACCAT+TGG | + | Chr3:93040971-93040990 | None:intergenic | 35.0% | |
| ! | AAGACCAATGGTTGGTTATA+TGG | - | Chr3:93040964-93040983 | MsG0380017179.01.T01:CDS | 35.0% |
| ! | ATTGGTTGATTGGTTAGTTG+AGG | - | Chr3:93041027-93041046 | MsG0380017179.01.T01:intron | 35.0% |
| ! | CAAAACAACTCTCTGTCTTT+TGG | + | Chr3:93040780-93040799 | None:intergenic | 35.0% |
| ! | CAAAAGACAGAGAGTTGTTT+TGG | - | Chr3:93040778-93040797 | MsG0380017179.01.T01:intron | 35.0% |
| ! | CATAGGTGTTATCAGTGATT+TGG | + | Chr3:93041324-93041343 | None:intergenic | 35.0% |
| ! | GATTGCTTCTGAGAATCAAA+AGG | - | Chr3:93041665-93041684 | MsG0380017179.01.T01:CDS | 35.0% |
| ! | TCTGGAACAGTTCAAATATG+TGG | - | Chr3:93042228-93042247 | MsG0380017179.01.T01:CDS | 35.0% |
| ! | TTTCTCCTTAACAGCTTTGA+AGG | + | Chr3:93042115-93042134 | None:intergenic | 35.0% |
| !! | ATTGACTTGAACTCATTGTC+TGG | - | Chr3:93042210-93042229 | MsG0380017179.01.T01:CDS | 35.0% |
| AAGATTCTTGCCTTCTGTTG+TGG | - | Chr3:93041847-93041866 | MsG0380017179.01.T01:CDS | 40.0% | |
| AGATCTAAGCTTCAGTTGCT+TGG | - | Chr3:93041136-93041155 | MsG0380017179.01.T01:CDS | 40.0% | |
| AGCATCCTTCAAAGCTGTTA+AGG | - | Chr3:93042107-93042126 | MsG0380017179.01.T01:CDS | 40.0% | |
| ATAATTTGGTATCTCTGGCG+GGG | + | Chr3:93042266-93042285 | None:intergenic | 40.0% | |
| ATCAAACATGAGAGGGACAT+TGG | - | Chr3:93041009-93041028 | MsG0380017179.01.T01:intron | 40.0% | |
| ATCTTACGATGCAATGAGCA+TGG | + | Chr3:93041170-93041189 | None:intergenic | 40.0% | |
| CAGAAATCTATAGCCTTTGG+TGG | + | Chr3:93041301-93041320 | None:intergenic | 40.0% | |
| GAAATCTATAGCCTTTGGTG+GGG | + | Chr3:93041299-93041318 | None:intergenic | 40.0% | |
| GGAGAAAAAGAGAAGACCAA+TGG | - | Chr3:93040952-93040971 | MsG0380017179.01.T01:CDS | 40.0% | |
| GGCATTACATCAAACATGAG+AGG | - | Chr3:93041001-93041020 | MsG0380017179.01.T01:intron | 40.0% | |
| GTAACCCAAGCATTCACAAA+GGG | + | Chr3:93042025-93042044 | None:intergenic | 40.0% | |
| TAACACCTATGATCTTGACG+AGG | - | Chr3:93041333-93041352 | MsG0380017179.01.T01:CDS | 40.0% | |
| TCAACAACAACCACATGTCA+TGG | - | Chr3:93040751-93040770 | MsG0380017179.01.T01:CDS | 40.0% | |
| TCATCCCTTTGTGAATGCTT+GGG | - | Chr3:93042018-93042037 | MsG0380017179.01.T01:CDS | 40.0% | |
| TGAAGAAATAACCCCACCAA+AGG | - | Chr3:93041285-93041304 | MsG0380017179.01.T01:intron | 40.0% | |
| TGAATGCTTGGGTTACAAGT+CGG | - | Chr3:93042029-93042048 | MsG0380017179.01.T01:CDS | 40.0% | |
| TGGCAGAAATCTATAGCCTT+TGG | + | Chr3:93041304-93041323 | None:intergenic | 40.0% | |
| TGTAACCCAAGCATTCACAA+AGG | + | Chr3:93042026-93042045 | None:intergenic | 40.0% | |
| TTCATCCCTTTGTGAATGCT+TGG | - | Chr3:93042017-93042036 | MsG0380017179.01.T01:CDS | 40.0% | |
| ! | GACATGTGGTTGTTGTTGAT+TGG | + | Chr3:93040750-93040769 | None:intergenic | 40.0% |
| ACAGAGAGATGAAGGGTTTC+TGG | + | Chr3:93041076-93041095 | None:intergenic | 45.0% | |
| TCTTGCGGATATAAGCCTGT+TGG | - | Chr3:93041811-93041830 | MsG0380017179.01.T01:intron | 45.0% | |
| TTTACCGAAGGATGTACTCC+AGG | + | Chr3:93041911-93041930 | None:intergenic | 45.0% | |
| ! | GATGAAGGGTTTCTGGAAGA+AGG | + | Chr3:93041069-93041088 | None:intergenic | 45.0% |
| ! | TGAGAGGGACATTGGTTGAT+TGG | - | Chr3:93041017-93041036 | MsG0380017179.01.T01:intron | 45.0% |
| !! | CGCTTGCTGTGATTCATTTC+AGG | - | Chr3:93041631-93041650 | MsG0380017179.01.T01:intron | 45.0% |
| !! | CTTTTGGGAACCATGACATG+TGG | + | Chr3:93040764-93040783 | None:intergenic | 45.0% |
| ACAGAAGCAGCCACAACAGA+AGG | + | Chr3:93041860-93041879 | None:intergenic | 50.0% | |
| GCTAGATTTATTCTCCGGCC+TGG | - | Chr3:93041890-93041909 | MsG0380017179.01.T01:CDS | 50.0% | |
| GGGTTACAAGTCGGCTGAAT+TGG | - | Chr3:93042038-93042057 | MsG0380017179.01.T01:CDS | 50.0% | |
| TTTGGTATCTCTGGCGGGGA+AGG | + | Chr3:93042262-93042281 | None:intergenic | 55.0% | |
| CCGAAGGATGTACTCCAGGC+CGG | + | Chr3:93041907-93041926 | None:intergenic | 60.0% | |
| CCGGCCTGGAGTACATCCTT+CGG | - | Chr3:93041904-93041923 | MsG0380017179.01.T01:CDS | 60.0% | |
| GTATCTCTGGCGGGGAAGGC+AGG | + | Chr3:93042258-93042277 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 93040713 | 93042303 | 93040713 | ID=MsG0380017179.01;Name=MsG0380017179.01 |
| Chr3 | mRNA | 93040713 | 93042303 | 93040713 | ID=MsG0380017179.01.T01;Parent=MsG0380017179.01;Name=MsG0380017179.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|7|0|335 |
| Chr3 | exon | 93041834 | 93042303 | 93041834 | ID=MsG0380017179.01.T01:exon:5361;Parent=MsG0380017179.01.T01 |
| Chr3 | exon | 93041662 | 93041737 | 93041662 | ID=MsG0380017179.01.T01:exon:5360;Parent=MsG0380017179.01.T01 |
| Chr3 | exon | 93041468 | 93041544 | 93041468 | ID=MsG0380017179.01.T01:exon:5359;Parent=MsG0380017179.01.T01 |
| Chr3 | exon | 93041330 | 93041363 | 93041330 | ID=MsG0380017179.01.T01:exon:5358;Parent=MsG0380017179.01.T01 |
| Chr3 | exon | 93041091 | 93041240 | 93041091 | ID=MsG0380017179.01.T01:exon:5357;Parent=MsG0380017179.01.T01 |
| Chr3 | exon | 93040867 | 93041001 | 93040867 | ID=MsG0380017179.01.T01:exon:5356;Parent=MsG0380017179.01.T01 |
| Chr3 | exon | 93040713 | 93040778 | 93040713 | ID=MsG0380017179.01.T01:exon:5355;Parent=MsG0380017179.01.T01 |
| Chr3 | CDS | 93041834 | 93042303 | 93041834 | ID=MsG0380017179.01.T01:cds;Parent=MsG0380017179.01.T01 |
| Chr3 | CDS | 93041662 | 93041737 | 93041662 | ID=MsG0380017179.01.T01:cds;Parent=MsG0380017179.01.T01 |
| Chr3 | CDS | 93041468 | 93041544 | 93041468 | ID=MsG0380017179.01.T01:cds;Parent=MsG0380017179.01.T01 |
| Chr3 | CDS | 93041330 | 93041363 | 93041330 | ID=MsG0380017179.01.T01:cds;Parent=MsG0380017179.01.T01 |
| Chr3 | CDS | 93041091 | 93041240 | 93041091 | ID=MsG0380017179.01.T01:cds;Parent=MsG0380017179.01.T01 |
| Chr3 | CDS | 93040867 | 93041001 | 93040867 | ID=MsG0380017179.01.T01:cds;Parent=MsG0380017179.01.T01 |
| Chr3 | CDS | 93040713 | 93040778 | 93040713 | ID=MsG0380017179.01.T01:cds;Parent=MsG0380017179.01.T01 |
| Gene Sequence |
| Protein sequence |
