AlfalfaGEDB | MsG0380014751.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014751.01

KEGG: K02213
GO: GO:0006270 GO:0051301
PFAM: PF09079 PF13401

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014751.01.T01	KEH34236.1	96.735	490	12	1	1	486	1	490	0	972
MsG0380014751.01.T02	KEH34236.1	96.735	490	12	1	1	486	1	490	0	972

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014751.01.T01	Q8W032	59.252	481	162	9	8	474	37	497	0	557
MsG0380014751.01.T02	Q8W032	59.252	481	162	9	8	474	37	497	0	557

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014751.01.T01	A0A072UXR1	96.735	490	12	1	1	486	1	490	0.0	972
MsG0380014751.01.T02	A0A072UXR1	96.735	490	12	1	1	486	1	490	0.0	972

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048775.01	MsG0380014751.01	0.829557	4.814983e-55	4.944926e-52
MsG0080048781.01	MsG0380014751.01	0.805198	1.498950e-49	7.881469e-47
MsG0180001293.01	MsG0380014751.01	0.810859	9.348543e-51	5.699099e-48
MsG0180002218.01	MsG0380014751.01	0.837963	3.811893e-57	5.032430e-54
MsG0180002397.01	MsG0380014751.01	0.840605	7.863721e-58	1.125866e-54
MsG0180003658.01	MsG0380014751.01	0.853416	2.424704e-61	5.235307e-58
MsG0180003857.01	MsG0380014751.01	0.833653	4.712017e-56	5.461320e-53
MsG0180004299.01	MsG0380014751.01	0.812970	3.243332e-51	2.091190e-48
MsG0180004538.01	MsG0380014751.01	0.829645	4.584386e-55	4.720599e-52
MsG0180004583.01	MsG0380014751.01	0.829547	4.843522e-55	4.972642e-52

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PPI

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Gene1	Gene2	Type
MsG0180000243.01	MsG0380014751.01	PPI
MsG0280009289.01	MsG0380014751.01	PPI
MsG0380014751.01	MsG0380017179.01	PPI
MsG0380014751.01	MsG0380017180.01	PPI
MsG0380014751.01	MsG0480022784.01	PPI
MsG0380014751.01	MsG0380017179.01	PPI
MsG0380014751.01	MsG0380017180.01	PPI
MsG0380014751.01	MsG0280009289.01	PPI
MsG0380014751.01	MsG0180000243.01	PPI
MsG0380014751.01	MsG0380014666.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014751.01.T01	MTR_3g463490	96.735	490	12	1	1	486	1	490	0.0	972
MsG0380014751.01.T01	MTR_3g463490	97.280	478	9	1	1	474	1	478	0.0	953
MsG0380014751.01.T01	MTR_8g099640	30.855	269	166	7	76	331	493	754	4.22e-23	103
MsG0380014751.01.T02	MTR_3g463490	96.735	490	12	1	1	486	1	490	0.0	972
MsG0380014751.01.T02	MTR_3g463490	97.280	478	9	1	1	474	1	478	0.0	953
MsG0380014751.01.T02	MTR_8g099640	30.855	269	166	7	76	331	493	754	4.22e-23	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014751.01.T01	AT1G07270	59.252	481	162	9	8	474	37	497	0.0	557
MsG0380014751.01.T01	AT2G29680	60.086	466	162	8	14	474	54	500	0.0	555
MsG0380014751.01.T01	AT2G29680	56.338	497	162	9	14	474	54	531	0.0	538
MsG0380014751.01.T01	AT1G07270	59.020	449	150	9	8	442	37	465	1.92e-179	513
MsG0380014751.01.T01	AT4G14700	31.461	267	165	7	72	328	417	675	4.77e-29	122
MsG0380014751.01.T01	AT4G12620	27.095	358	220	10	72	419	422	748	1.78e-25	111
MsG0380014751.01.T02	AT1G07270	59.252	481	162	9	8	474	37	497	0.0	557
MsG0380014751.01.T02	AT2G29680	60.086	466	162	8	14	474	54	500	0.0	555
MsG0380014751.01.T02	AT2G29680	56.338	497	162	9	14	474	54	531	0.0	538
MsG0380014751.01.T02	AT1G07270	59.020	449	150	9	8	442	37	465	1.92e-179	513

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Find 7 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTATGGTTTAAATGATGT+TGG	0.359726	3:+59425624	None:intergenic
ATGTTGCCAGAACCTGTAAG+CGG	0.675706	3:+59425843	None:intergenic
ATTATGGTTTAAATGATGTT+GGG	0.414663	3:+59425625	None:intergenic
GTGATCCCGCTTACAGGTTC+TGG	0.321911	3:-59425849	MsG0380014751.01.T01:CDS
TCAGAAGCAAGAGATGTTAG+AGG	0.342938	3:-59425818	MsG0380014751.01.T01:CDS
TCTTCAGTGATCCCGCTTAC+AGG	0.310267	3:-59425855	MsG0380014751.01.T01:CDS
TGTTGCCAGAACCTGTAAGC+GGG	0.554297	3:+59425844	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATTTATATAACTCGCCA+AGG	+	Chr3:59429553-59429572	None:intergenic	30.0%
AAGCATGTCAATCAAATCAA+CGG	+	Chr3:59426852-59426871	None:intergenic	30.0%
ACAATTTACGGATAAAGCAT+CGG	+	Chr3:59427174-59427193	None:intergenic	30.0%
ATGAATGTGTTCTGTATTTC+AGG	-	Chr3:59428747-59428766	MsG0380014751.01.T01:intron	30.0%
CAAATATTGCAACTTGCAAT+TGG	+	Chr3:59428653-59428672	None:intergenic	30.0%
CATATCACATTGTTCTCTAT+TGG	+	Chr3:59426999-59427018	None:intergenic	30.0%
CTCTTCTCAATTTATCATCT+CGG	+	Chr3:59429801-59429820	None:intergenic	30.0%
CTTATTGTGCTTGTTTATAC+AGG	-	Chr3:59427756-59427775	MsG0380014751.01.T01:intron	30.0%
GAAAGCGAATTTACTCAATT+GGG	-	Chr3:59426318-59426337	MsG0380014751.01.T01:CDS	30.0%
GAGTAATTAAAAAGAGAGCA+AGG	+	Chr3:59427959-59427978	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	59425637	59430250	59425637	ID=MsG0380014751.01;Name=MsG0380014751.01
Chr3	mRNA	59425637	59430250	59425637	ID=MsG0380014751.01.T01;Parent=MsG0380014751.01;Name=MsG0380014751.01.T01;_AED=0.31;_eAED=0.32;_QI=76\|0.92\|0.92\|1\|0.84\|0.78\|14\|117\|521
Chr3	exon	59425637	59425708	59425637	ID=MsG0380014751.01.T01:exon:11557;Parent=MsG0380014751.01.T01
Chr3	exon	59425732	59425900	59425732	ID=MsG0380014751.01.T01:exon:11556;Parent=MsG0380014751.01.T01
Chr3	five_prime_UTR	59430175	59430250	59430175	ID=MsG0380014751.01.T01:five_prime_utr;Parent=MsG0380014751.01.T01
Chr3	CDS	59429970	59430174	59429970	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59429539	59429759	59429539	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59428658	59428732	59428658	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59428454	59428554	59428454	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59428218	59428324	59428218	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59428050	59428109	59428050	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59427819	59427892	59427819	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59427567	59427617	59427567	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59427413	59427462	59427413	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59426986	59427118	59426986	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59426232	59426480	59426232	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59425997	59426112	59425997	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	CDS	59425777	59425900	59425777	ID=MsG0380014751.01.T01:cds;Parent=MsG0380014751.01.T01
Chr3	three_prime_UTR	59425732	59425776	59425732	ID=MsG0380014751.01.T01:three_prime_utr;Parent=MsG0380014751.01.T01
Chr3	three_prime_UTR	59425637	59425708	59425637	ID=MsG0380014751.01.T01:three_prime_utr;Parent=MsG0380014751.01.T01
Chr3	mRNA	59425721	59430250	59425721	ID=MsG0380014751.01.T02;Parent=MsG0380014751.01;Name=MsG0380014751.01.T02;_AED=0.32;_eAED=0.32;_QI=76\|0.91\|0.92\|1\|0.91\|0.84\|13\|56\|521
Chr3	exon	59425721	59425900	59425721	ID=MsG0380014751.01.T02:exon:11558;Parent=MsG0380014751.01.T02
Chr3	five_prime_UTR	59430175	59430250	59430175	ID=MsG0380014751.01.T02:five_prime_utr;Parent=MsG0380014751.01.T02
Chr3	CDS	59429970	59430174	59429970	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59429539	59429759	59429539	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59428658	59428732	59428658	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59428454	59428554	59428454	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59428218	59428324	59428218	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59428050	59428109	59428050	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59427819	59427892	59427819	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59427567	59427617	59427567	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59427413	59427462	59427413	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59426986	59427118	59426986	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59426232	59426480	59426232	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59425997	59426112	59425997	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	CDS	59425777	59425900	59425777	ID=MsG0380014751.01.T02:cds;Parent=MsG0380014751.01.T02
Chr3	three_prime_UTR	59425721	59425776	59425721	ID=MsG0380014751.01.T02:three_prime_utr;Parent=MsG0380014751.01.T02

Gene Sequence

>MsG0380014751.01.T01

ATGCCTTCAATTGCTTCATCCAAACGCAGATTCAGATCTCCCGCCTCACCCATCCCGACCACCCCTACCCGGAAATCCCCCCGCCGTTGCATCTCTGCAACTCCCAATTCAGTCGCCTCATCCGTTGTTCGTCAAATCATCACCGATGAAAATCAAATCGAATCGATCCAACCTGCATCTCTTAAATCCAAATGGAACCCCAAAGATGGTGAGCAAATGAGGATTGTGAAGGAGGTGTTGCATTTGTCTACAGCACCGTCTACGATGGTGTGTCGTGAGGAAGAGCAAAATGTGGTGTTGGGGTTTTGTAAAGGCTGTGTTGAGCATGAAAAAGCCGGGAGTCTTTATATATGTGGCTGTCCTGGAACTGGGAAATCGTTGTCGATGGAGAAAGTGAAAGCGAATTTACTCAATTGGGCTGCAGAGGGAGGTTTTCCGCTGCCCGATGCTTTATCCGTAAATTGTACATCACTGTCAAATACATCTGATATTTTCACAAAGATACTTGGGTTACATAAAACCCTGGGGAAAAAGGGTTCAGGCACACCCTTACAGCAACTTCAGAACTTGTATTCTCATAAATCACCTACATCGAACATGATATTGATAGTAGCAGATGAATTAGATTATCTAATTACCAAAGACAGAGCTGTGCTCCATGATCTTTTCATGCTTACCACTTTTCCATTTTCCAGATGTATACTTATAGGTGTAGCAAATGCAATTGATCTAGCTGATCGATTTCTTCCAAGGCTTACTGCATTAAATTGCAAGCCTACTGTTGTAACTTTTCGGGCATATACTAAAGATCAGATCCTTAAGATTATTCAAGAAAGGCTAAATGAACTTCCTTACATCGTCTTCCAACATCAAGCATTGGAACTATGTGCCAGGAAAGTTGCTGCAGCTTCTGGAGATATGCGAAATGCTCTTTGTATATGCAGGAGTGCAATAGAGATGCTAGAAGCAGAAATTAGAGATTCTGCTTGCAATTTGAATGCTTTGGAAGAGAAATCATCCTCAGAACAAAATCTTCCTACAGCACCGGATTGTGTTAAAAAACAAGAATTTGATATGGTAAGGATTGATCATATGGCTCTTGCATTGTCCAAAACATATAGATCACCAGTGGTGGATACCATACAATCTCTGCCACATCATCAACAAATTATACTCTGCTCCTCGATGAAACGTTTCCGTGGAGCCAAGAAAGACTCAATCCTTGGCGAGTTATATAAATCTTATGCGGAGATATGCAAATCATCACTAATTCCCCCTGCTGGAATCATGGAATTTTCAAACATGTGCAGAGTGTTAAATGACCAGGGCCTTATTAAACTAGGACAATCCCGAGATGATAAATTGAGAAGAGTGACACCTAAACTAGATGAAGGTGATATTACTTTTGCATTGCAGGTAATTTTTCATTCCCTTGAGCCACTGGAATCCGCTTTTTTCGAAATTTTCTTCAGTGATCCCGCTTACAGGTTCTGGCAACATTTTCAGAAGCAAGAGATGTTAGAGGAATTTTATTTATTTTTTCTAGAACAAAAATATAGCAATTGA

>MsG0380014751.01.T02

Protein sequence

>MsG0380014751.01.T01

MPSIASSKRRFRSPASPIPTTPTRKSPRRCISATPNSVASSVVRQIITDENQIESIQPASLKSKWNPKDGEQMRIVKEVLHLSTAPSTMVCREEEQNVVLGFCKGCVEHEKAGSLYICGCPGTGKSLSMEKVKANLLNWAAEGGFPLPDALSVNCTSLSNTSDIFTKILGLHKTLGKKGSGTPLQQLQNLYSHKSPTSNMILIVADELDYLITKDRAVLHDLFMLTTFPFSRCILIGVANAIDLADRFLPRLTALNCKPTVVTFRAYTKDQILKIIQERLNELPYIVFQHQALELCARKVAAASGDMRNALCICRSAIEMLEAEIRDSACNLNALEEKSSSEQNLPTAPDCVKKQEFDMVRIDHMALALSKTYRSPVVDTIQSLPHHQQIILCSSMKRFRGAKKDSILGELYKSYAEICKSSLIPPAGIMEFSNMCRVLNDQGLIKLGQSRDDKLRRVTPKLDEGDITFALQVIFHSLEPLESAFFEIFFSDPAYRFWQHFQKQEMLEEFYLFFLEQKYSN*

>MsG0380014751.01.T02