Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017583.01.T01 | XP_003603585.1 | 99.608 | 255 | 1 | 0 | 1 | 255 | 1 | 255 | 0 | 529 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017583.01.T01 | Q8GXB7 | 79.835 | 243 | 46 | 2 | 8 | 249 | 8 | 248 | 7.39E-146 | 410 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017583.01.T01 | G7J2W7 | 99.608 | 255 | 1 | 0 | 1 | 255 | 1 | 255 | 0.0 | 529 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000280.01 | MsG0380017583.01 | 0.820857 | 5.494434e-53 | 4.398639e-50 |
| MsG0380012279.01 | MsG0380017583.01 | 0.809309 | 2.016271e-50 | 1.179744e-47 |
| MsG0380016201.01 | MsG0380017583.01 | 0.808983 | 2.368311e-50 | 1.373664e-47 |
| MsG0380017583.01 | MsG0780040576.01 | 0.816318 | 5.881834e-52 | 4.154372e-49 |
| MsG0380017583.01 | MsG0880045707.01 | 0.804523 | 2.073326e-49 | 1.071315e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880045124.01 | MsG0380017583.01 | PPI |
| MsG0780040655.01 | MsG0380017583.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017583.01.T01 | MTR_3g109360 | 99.608 | 255 | 1 | 0 | 1 | 255 | 1 | 255 | 0.0 | 529 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017583.01.T01 | AT5G24840 | 79.835 | 243 | 46 | 2 | 8 | 249 | 8 | 248 | 7.53e-147 | 410 |
Find 65 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATTAAGTTTGCCGATAT+TGG | 0.221557 | 3:+98107933 | MsG0380017583.01.T01:CDS |
| TCCGCACCACAGAAACATTT+TGG | 0.250656 | 3:-98108097 | None:intergenic |
| ATTCCGATCATCAATGTTTC+TGG | 0.252188 | 3:-98107999 | None:intergenic |
| GCCGATATTGGCTGTGGTTT+TGG | 0.274610 | 3:+98107945 | MsG0380017583.01.T01:CDS |
| CCTCGTTTCTAGCAACCTTT+TGG | 0.326676 | 3:-98108427 | None:intergenic |
| TAAAATGAGGATCGGGAAAC+AGG | 0.368009 | 3:-98108181 | None:intergenic |
| TTCTCTTTAAAATGAGGATC+GGG | 0.398927 | 3:-98108188 | None:intergenic |
| GCATACTCATCTAGTAAGAA+TGG | 0.400409 | 3:-98108236 | None:intergenic |
| AAACGAGGGACAAACTTTCC+AGG | 0.404412 | 3:+98108442 | MsG0380017583.01.T01:CDS |
| TTGTGCTTTCTCAAAGTGAT+TGG | 0.404689 | 3:-98108141 | None:intergenic |
| GGACTGGATGAAATCTTGTT+TGG | 0.409104 | 3:+98108316 | MsG0380017583.01.T01:CDS |
| TCCAAAACCACAGCCAATAT+CGG | 0.417152 | 3:-98107946 | None:intergenic |
| TTAAGCTCTGCCACTGAAGA+AGG | 0.428496 | 3:+98108404 | MsG0380017583.01.T01:CDS |
| TGTGCTTTCTCAAAGTGATT+GGG | 0.444654 | 3:-98108140 | None:intergenic |
| TCAACATTCAGTAAGTCAAC+AGG | 0.452366 | 3:+98107759 | MsG0380017583.01.T01:CDS |
| GTGATTGGGAATGTACTTCA+TGG | 0.454613 | 3:-98108126 | None:intergenic |
| GTTGAATTTCCACCAGTCTC+AGG | 0.455044 | 3:-98107741 | None:intergenic |
| TGGTTCTTCTCTTTAAAATG+AGG | 0.455252 | 3:-98108194 | None:intergenic |
| CAGTCAAAGGTTCAAACAAC+GGG | 0.464877 | 3:-98108346 | None:intergenic |
| TAAAGTGTCCGAGTATGTTA+AGG | 0.471708 | 3:+98108034 | MsG0380017583.01.T01:CDS |
| TCAAGTTCTTTCTCAGTCAA+AGG | 0.473439 | 3:-98108359 | None:intergenic |
| ATAACAGATGTGGAAGAACT+TGG | 0.476880 | 3:+98108293 | MsG0380017583.01.T01:CDS |
| GCTCGATAGAAGCGCTTTCG+AGG | 0.477207 | 3:-98107786 | None:intergenic |
| TCTTTAAGGGTAGCAAATCC+AGG | 0.486768 | 3:+98108068 | MsG0380017583.01.T01:CDS |
| GAAAGAACTTGAAGCAGATC+CGG | 0.487319 | 3:+98108370 | MsG0380017583.01.T01:CDS |
| TTCCGATCATCAATGTTTCT+GGG | 0.488618 | 3:-98107998 | None:intergenic |
| AGAGAAGAACCATCGCCGTC+GGG | 0.492041 | 3:+98108205 | MsG0380017583.01.T01:CDS |
| GAGGGAATAGTCAACATGAC+TGG | 0.493842 | 3:-98107862 | None:intergenic |
| CTTCTCTTTAAAATGAGGAT+CGG | 0.494124 | 3:-98108189 | None:intergenic |
| CATACTCATCTAGTAAGAAT+GGG | 0.503167 | 3:-98108235 | None:intergenic |
| TGCCACTGAAGAAGGCCAAA+AGG | 0.507812 | 3:+98108412 | MsG0380017583.01.T01:CDS |
| TCAACATGACTGGGTGATAA+CGG | 0.513146 | 3:-98107852 | None:intergenic |
| AGGGAAAGAACTGAGGGTAA+TGG | 0.514591 | 3:-98107884 | None:intergenic |
| AAGTTTGCCGATATTGGCTG+TGG | 0.514722 | 3:+98107939 | MsG0380017583.01.T01:CDS |
| GAAAGAACTGAGGGTAATGG+AGG | 0.517145 | 3:-98107881 | None:intergenic |
| TAACAGATGTGGAAGAACTT+GGG | 0.526686 | 3:+98108294 | MsG0380017583.01.T01:CDS |
| AAGAGAAGAACCATCGCCGT+CGG | 0.534336 | 3:+98108204 | MsG0380017583.01.T01:CDS |
| CTATCAGCTTTCTCAGACGA+GGG | 0.534942 | 3:-98107903 | None:intergenic |
| CAATGCGTCTGTATACAGCC+TGG | 0.550730 | 3:-98108460 | None:intergenic |
| GGCTGATAACCCGACGGCGA+TGG | 0.555787 | 3:-98108214 | None:intergenic |
| TAAACAGACATGCCTGAGAC+TGG | 0.565306 | 3:+98107729 | None:intergenic |
| ACCAAAATGTTTCTGTGGTG+CGG | 0.567556 | 3:+98108096 | MsG0380017583.01.T01:CDS |
| AATTCGTTCCTTAACATACT+CGG | 0.570956 | 3:-98108042 | None:intergenic |
| TTCCCAGAAACATTGATGAT+CGG | 0.572410 | 3:+98107996 | MsG0380017583.01.T01:CDS |
| GAGCTTAAAAGCTTAACAGC+CGG | 0.573038 | 3:-98108389 | None:intergenic |
| ATGTGGAAGAACTTGGGGAC+TGG | 0.574117 | 3:+98108300 | MsG0380017583.01.T01:CDS |
| TGACTGGGTGATAACGGCAC+AGG | 0.576851 | 3:-98107846 | None:intergenic |
| AGAAACATTGATGATCGGAA+TGG | 0.582107 | 3:+98108001 | MsG0380017583.01.T01:CDS |
| AAAGAACTGAGGGTAATGGA+GGG | 0.599329 | 3:-98107880 | None:intergenic |
| TATATACACAATAACAGATG+TGG | 0.600775 | 3:+98108283 | MsG0380017583.01.T01:CDS |
| AACAGATGTGGAAGAACTTG+GGG | 0.605004 | 3:+98108295 | MsG0380017583.01.T01:CDS |
| GGAAATGAGAGTCACTGAGC+GGG | 0.605858 | 3:-98107824 | None:intergenic |
| GACTGGGTGATAACGGCACA+GGG | 0.611699 | 3:-98107845 | None:intergenic |
| TCAGTCAAAGGTTCAAACAA+CGG | 0.617042 | 3:-98108347 | None:intergenic |
| TCAATACCAAAATGTTTCTG+TGG | 0.618646 | 3:+98108091 | MsG0380017583.01.T01:CDS |
| CCAAAAGGTTGCTAGAAACG+AGG | 0.621682 | 3:+98108427 | MsG0380017583.01.T01:CDS |
| AGGGAATAGTCAACATGACT+GGG | 0.631622 | 3:-98107861 | None:intergenic |
| ACAGACATGCCTGAGACTGG+TGG | 0.638512 | 3:+98107732 | None:intergenic |
| GGGAAATGAGAGTCACTGAG+CGG | 0.640601 | 3:-98107825 | None:intergenic |
| CAAAAGGTTGCTAGAAACGA+GGG | 0.641444 | 3:+98108428 | MsG0380017583.01.T01:CDS |
| ACTATCAGCTTTCTCAGACG+AGG | 0.648094 | 3:-98107904 | None:intergenic |
| CAATGTTTCTGGGAACAGAG+TGG | 0.655902 | 3:-98107988 | None:intergenic |
| CAGACGAGGGAAAGAACTGA+GGG | 0.659441 | 3:-98107890 | None:intergenic |
| TCAGACGAGGGAAAGAACTG+AGG | 0.695327 | 3:-98107891 | None:intergenic |
| AGAATGGGCTGATAACCCGA+CGG | 0.724299 | 3:-98108220 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATATACACAATAACAGATG+TGG | + | Chr3:98108283-98108302 | MsG0380017583.01.T01:CDS | 25.0% |
| !! | AGGAACGAATTTTATCTTTA+AGG | + | Chr3:98108054-98108073 | MsG0380017583.01.T01:CDS | 25.0% |
| !! | GGAACGAATTTTATCTTTAA+GGG | + | Chr3:98108055-98108074 | MsG0380017583.01.T01:CDS | 25.0% |
| AAGATTAAGTTTGCCGATAT+TGG | + | Chr3:98107933-98107952 | MsG0380017583.01.T01:CDS | 30.0% | |
| AATTCGTTCCTTAACATACT+CGG | - | Chr3:98108045-98108064 | None:intergenic | 30.0% | |
| CATACTCATCTAGTAAGAAT+GGG | - | Chr3:98108238-98108257 | None:intergenic | 30.0% | |
| ! | TCAATACCAAAATGTTTCTG+TGG | + | Chr3:98108091-98108110 | MsG0380017583.01.T01:CDS | 30.0% |
| !! | CTTCTCTTTAAAATGAGGAT+CGG | - | Chr3:98108192-98108211 | None:intergenic | 30.0% |
| !! | TGGTTCTTCTCTTTAAAATG+AGG | - | Chr3:98108197-98108216 | None:intergenic | 30.0% |
| !! | TTCTCTTTAAAATGAGGATC+GGG | - | Chr3:98108191-98108210 | None:intergenic | 30.0% |
| ATAACAGATGTGGAAGAACT+TGG | + | Chr3:98108293-98108312 | MsG0380017583.01.T01:CDS | 35.0% | |
| ATTCCGATCATCAATGTTTC+TGG | - | Chr3:98108002-98108021 | None:intergenic | 35.0% | |
| GCATACTCATCTAGTAAGAA+TGG | - | Chr3:98108239-98108258 | None:intergenic | 35.0% | |
| TAACAGATGTGGAAGAACTT+GGG | + | Chr3:98108294-98108313 | MsG0380017583.01.T01:CDS | 35.0% | |
| TCAACATTCAGTAAGTCAAC+AGG | + | Chr3:98107759-98107778 | MsG0380017583.01.T01:CDS | 35.0% | |
| TCAAGTTCTTTCTCAGTCAA+AGG | - | Chr3:98108362-98108381 | None:intergenic | 35.0% | |
| TCAGTCAAAGGTTCAAACAA+CGG | - | Chr3:98108350-98108369 | None:intergenic | 35.0% | |
| TGTGCTTTCTCAAAGTGATT+GGG | - | Chr3:98108143-98108162 | None:intergenic | 35.0% | |
| TTCCGATCATCAATGTTTCT+GGG | - | Chr3:98108001-98108020 | None:intergenic | 35.0% | |
| TTGTGCTTTCTCAAAGTGAT+TGG | - | Chr3:98108144-98108163 | None:intergenic | 35.0% | |
| ! | AGAAACATTGATGATCGGAA+TGG | + | Chr3:98108001-98108020 | MsG0380017583.01.T01:CDS | 35.0% |
| ! | TAAAGTGTCCGAGTATGTTA+AGG | + | Chr3:98108034-98108053 | MsG0380017583.01.T01:CDS | 35.0% |
| ! | TATGCTTTTGTTCTTGAGGT+TGG | + | Chr3:98108254-98108273 | MsG0380017583.01.T01:CDS | 35.0% |
| ! | TGAGTATGCTTTTGTTCTTG+AGG | + | Chr3:98108250-98108269 | MsG0380017583.01.T01:CDS | 35.0% |
| ! | TTCCCAGAAACATTGATGAT+CGG | + | Chr3:98107996-98108015 | MsG0380017583.01.T01:CDS | 35.0% |
| !!! | GAAACATTTTGGTATTGACC+TGG | - | Chr3:98108089-98108108 | None:intergenic | 35.0% |
| AACAGATGTGGAAGAACTTG+GGG | + | Chr3:98108295-98108314 | MsG0380017583.01.T01:CDS | 40.0% | |
| AGGGAATAGTCAACATGACT+GGG | - | Chr3:98107864-98107883 | None:intergenic | 40.0% | |
| CAAAAGGTTGCTAGAAACGA+GGG | + | Chr3:98108428-98108447 | MsG0380017583.01.T01:CDS | 40.0% | |
| CAGTCAAAGGTTCAAACAAC+GGG | - | Chr3:98108349-98108368 | None:intergenic | 40.0% | |
| GAAAGAACTTGAAGCAGATC+CGG | + | Chr3:98108370-98108389 | MsG0380017583.01.T01:CDS | 40.0% | |
| GAGCTTAAAAGCTTAACAGC+CGG | - | Chr3:98108392-98108411 | None:intergenic | 40.0% | |
| GGACTGGATGAAATCTTGTT+TGG | + | Chr3:98108316-98108335 | MsG0380017583.01.T01:CDS | 40.0% | |
| GTGATTGGGAATGTACTTCA+TGG | - | Chr3:98108129-98108148 | None:intergenic | 40.0% | |
| TAAAATGAGGATCGGGAAAC+AGG | - | Chr3:98108184-98108203 | None:intergenic | 40.0% | |
| TCAACATGACTGGGTGATAA+CGG | - | Chr3:98107855-98107874 | None:intergenic | 40.0% | |
| TCCAAAACCACAGCCAATAT+CGG | - | Chr3:98107949-98107968 | None:intergenic | 40.0% | |
| ! | ACCAAAATGTTTCTGTGGTG+CGG | + | Chr3:98108096-98108115 | MsG0380017583.01.T01:CDS | 40.0% |
| ! | TCTTTAAGGGTAGCAAATCC+AGG | + | Chr3:98108068-98108087 | MsG0380017583.01.T01:CDS | 40.0% |
| !! | AAAGAACTGAGGGTAATGGA+GGG | - | Chr3:98107883-98107902 | None:intergenic | 40.0% |
| !!! | ATATTGGCTGTGGTTTTGGA+GGG | + | Chr3:98107949-98107968 | MsG0380017583.01.T01:CDS | 40.0% |
| AAACGAGGGACAAACTTTCC+AGG | + | Chr3:98108442-98108461 | MsG0380017583.01.T01:CDS | 45.0% | |
| ACTATCAGCTTTCTCAGACG+AGG | - | Chr3:98107907-98107926 | None:intergenic | 45.0% | |
| AGGGAAAGAACTGAGGGTAA+TGG | - | Chr3:98107887-98107906 | None:intergenic | 45.0% | |
| CCAAAAGGTTGCTAGAAACG+AGG | + | Chr3:98108427-98108446 | MsG0380017583.01.T01:CDS | 45.0% | |
| CTATCAGCTTTCTCAGACGA+GGG | - | Chr3:98107906-98107925 | None:intergenic | 45.0% | |
| GAGGGAATAGTCAACATGAC+TGG | - | Chr3:98107865-98107884 | None:intergenic | 45.0% | |
| GTTGAATTTCCACCAGTCTC+AGG | - | Chr3:98107744-98107763 | None:intergenic | 45.0% | |
| TTAAGCTCTGCCACTGAAGA+AGG | + | Chr3:98108404-98108423 | MsG0380017583.01.T01:CDS | 45.0% | |
| ! | AAGTTTGCCGATATTGGCTG+TGG | + | Chr3:98107939-98107958 | MsG0380017583.01.T01:CDS | 45.0% |
| ! | GAAAGAACTGAGGGTAATGG+AGG | - | Chr3:98107884-98107903 | None:intergenic | 45.0% |
| ! | GCTTTTGTTCTTGAGGTTGG+TGG | + | Chr3:98108257-98108276 | MsG0380017583.01.T01:CDS | 45.0% |
| ! | TCCGCACCACAGAAACATTT+TGG | - | Chr3:98108100-98108119 | None:intergenic | 45.0% |
| !! | AACCTTTTGGCCTTCTTCAG+TGG | - | Chr3:98108417-98108436 | None:intergenic | 45.0% |
| !! | CAATGTTTCTGGGAACAGAG+TGG | - | Chr3:98107991-98108010 | None:intergenic | 45.0% |
| !!! | CCTCGTTTCTAGCAACCTTT+TGG | - | Chr3:98108430-98108449 | None:intergenic | 45.0% |
| !!! | GATATTGGCTGTGGTTTTGG+AGG | + | Chr3:98107948-98107967 | MsG0380017583.01.T01:CDS | 45.0% |
| AAGAGAAGAACCATCGCCGT+CGG | + | Chr3:98108204-98108223 | MsG0380017583.01.T01:CDS | 50.0% | |
| ATGTGGAAGAACTTGGGGAC+TGG | + | Chr3:98108300-98108319 | MsG0380017583.01.T01:CDS | 50.0% | |
| CAATGCGTCTGTATACAGCC+TGG | - | Chr3:98108463-98108482 | None:intergenic | 50.0% | |
| CAGACGAGGGAAAGAACTGA+GGG | - | Chr3:98107893-98107912 | None:intergenic | 50.0% | |
| GGAAATGAGAGTCACTGAGC+GGG | - | Chr3:98107827-98107846 | None:intergenic | 50.0% | |
| GGGAAATGAGAGTCACTGAG+CGG | - | Chr3:98107828-98107847 | None:intergenic | 50.0% | |
| TCAGACGAGGGAAAGAACTG+AGG | - | Chr3:98107894-98107913 | None:intergenic | 50.0% | |
| TGCCACTGAAGAAGGCCAAA+AGG | + | Chr3:98108412-98108431 | MsG0380017583.01.T01:CDS | 50.0% | |
| !! | AGAATGGGCTGATAACCCGA+CGG | - | Chr3:98108223-98108242 | None:intergenic | 50.0% |
| !! | GCCGATATTGGCTGTGGTTT+TGG | + | Chr3:98107945-98107964 | MsG0380017583.01.T01:CDS | 50.0% |
| AGAGAAGAACCATCGCCGTC+GGG | + | Chr3:98108205-98108224 | MsG0380017583.01.T01:CDS | 55.0% | |
| !! | GACTGGGTGATAACGGCACA+GGG | - | Chr3:98107848-98107867 | None:intergenic | 55.0% |
| !! | GCTCGATAGAAGCGCTTTCG+AGG | - | Chr3:98107789-98107808 | None:intergenic | 55.0% |
| !! | TGACTGGGTGATAACGGCAC+AGG | - | Chr3:98107849-98107868 | None:intergenic | 55.0% |
| GGCTGATAACCCGACGGCGA+TGG | - | Chr3:98108217-98108236 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 98107738 | 98108505 | 98107738 | ID=MsG0380017583.01;Name=MsG0380017583.01 |
| Chr3 | mRNA | 98107738 | 98108505 | 98107738 | ID=MsG0380017583.01.T01;Parent=MsG0380017583.01;Name=MsG0380017583.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|255 |
| Chr3 | exon | 98107738 | 98108505 | 98107738 | ID=MsG0380017583.01.T01:exon:6170;Parent=MsG0380017583.01.T01 |
| Chr3 | CDS | 98107738 | 98108505 | 98107738 | ID=MsG0380017583.01.T01:cds;Parent=MsG0380017583.01.T01 |
| Gene Sequence |
| Protein sequence |
