AlfalfaGEDB | MsG0380017999.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017999.01

KEGG: -
GO: GO:0003677
PFAM: PF00249

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017999.01.T01	XP_024633411.1	100	81	0	0	1	81	1	81	9.27E-53	169

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017999.01.T01	Q1A173	67.5	80	22	1	1	80	1	76	2.92E-34	115

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017999.01.T01	G7J732	100.000	81	0	0	1	81	1	81	4.43e-53	169

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002982.01	MsG0380017999.01	0.805837	1.100874e-49	5.884358e-47
MsG0180005806.01	MsG0380017999.01	0.807265	5.500925e-50	3.050213e-47
MsG0380017999.01	MsG0480018100.01	0.800637	1.312994e-48	6.144513e-46
MsG0380017999.01	MsG0480019693.01	0.803448	3.470054e-49	1.744217e-46
MsG0380017999.01	MsG0580028092.01	0.806427	8.271777e-50	4.488414e-47
MsG0380017999.01	MsG0680035612.01	0.801074	1.068804e-48	5.057811e-46
MsG0380017999.01	MsG0780039179.01	0.800738	1.251994e-48	5.874139e-46
MsG0380017999.01	MsG0880042921.01	0.803860	2.849133e-49	1.447417e-46
MsG0380017999.01	MsG0880045772.01	0.813279	2.774568e-51	1.804048e-48
MsG0380017999.01	MsG0880046584.01	0.808309	3.298997e-50	1.879957e-47
MsG0380017999.01	MsG0880046639.01	0.809966	1.456858e-50	8.673970e-48
MsG0280008233.01	MsG0380017999.01	0.812419	4.280713e-51	2.720508e-48
MsG0280010523.01	MsG0380017999.01	0.811164	8.030102e-51	4.934703e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017999.01.T01	MTR_3g116720	100.000	81	0	0	1	81	1	81	1.12e-56	169
MsG0380017999.01.T01	MTR_1g022290	73.134	67	18	0	14	80	5	71	2.53e-34	112
MsG0380017999.01.T01	MTR_3g111880	73.846	65	17	0	14	78	5	69	8.81e-32	106
MsG0380017999.01.T01	MTR_7g089210	77.049	61	14	0	19	79	15	75	2.92e-30	102
MsG0380017999.01.T01	MTR_3g013440	62.963	81	23	2	1	80	1	75	6.61e-29	99.4
MsG0380017999.01.T01	MTR_8g077360	64.615	65	22	1	16	80	5	68	2.45e-27	94.7
MsG0380017999.01.T01	MTR_8g077380	64.615	65	22	1	16	80	5	68	2.52e-27	94.7
MsG0380017999.01.T01	MTR_8g077390	64.615	65	22	1	16	80	5	68	2.52e-27	94.7
MsG0380017999.01.T01	MTR_5g020170	60.938	64	25	0	16	79	19	82	5.64e-26	92.0
MsG0380017999.01.T01	MTR_6g004250	60.000	65	26	0	14	78	8	72	1.11e-25	90.9
MsG0380017999.01.T01	MTR_5g020540	68.852	61	18	1	16	76	4	63	1.62e-25	89.7
MsG0380017999.01.T01	MTR_8g077420	63.492	63	22	1	14	76	3	64	2.75e-25	89.4
MsG0380017999.01.T01	MTR_3g111920	53.846	65	30	0	16	80	3	67	7.28e-22	81.3
MsG0380017999.01.T01	MTR_8g063870	50.725	69	34	0	12	80	4	72	1.32e-20	82.4
MsG0380017999.01.T01	MTR_3g462790	57.576	66	28	0	16	81	43	108	2.76e-20	82.4
MsG0380017999.01.T01	MTR_5g069710	53.846	65	30	0	16	80	29	93	9.73e-20	80.9
MsG0380017999.01.T01	MTR_1g033600	48.529	68	35	0	14	81	10	77	1.03e-19	77.4
MsG0380017999.01.T01	MTR_8g063600	50.769	65	32	0	16	80	10	74	1.68e-19	79.7
MsG0380017999.01.T01	MTR_7g067080	51.471	68	33	0	14	81	33	100	2.10e-18	77.0
MsG0380017999.01.T01	MTR_5g081860	53.968	63	29	0	16	78	27	89	3.86e-18	75.9
MsG0380017999.01.T01	MTR_1g086180	59.649	57	22	1	16	71	11	67	3.08e-16	67.4
MsG0380017999.01.T01	MTR_1g111830	47.619	63	33	0	16	78	24	86	7.24e-16	69.7
MsG0380017999.01.T01	MTR_7g110580	57.627	59	24	1	16	73	8	66	4.52e-15	63.9
MsG0380017999.01.T01	MTR_4g111975	41.176	68	37	1	14	78	6	73	1.28e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017999.01.T01	AT1G75250	67.500	80	22	1	1	80	1	76	2.38e-35	116
MsG0380017999.01.T01	AT1G75250	67.500	80	22	1	1	80	1	76	2.98e-35	115
MsG0380017999.01.T01	AT4G39250	69.697	66	20	0	16	81	14	79	8.62e-34	112
MsG0380017999.01.T01	AT2G21650	66.667	66	22	0	16	81	14	79	1.88e-30	103
MsG0380017999.01.T01	AT1G19510	71.429	63	18	0	19	81	15	77	1.03e-29	101
MsG0380017999.01.T01	AT2G18328	69.841	63	19	0	19	81	14	76	7.58e-29	98.6
MsG0380017999.01.T01	AT4G36570	77.273	44	10	0	16	59	12	55	1.94e-21	79.3
MsG0380017999.01.T01	AT5G05790	52.941	68	32	0	14	81	29	96	2.78e-18	76.6
MsG0380017999.01.T01	AT5G05790	52.941	68	32	0	14	81	29	96	2.78e-18	76.6
MsG0380017999.01.T01	AT2G38090	53.846	65	30	0	16	80	29	93	1.45e-17	74.7
MsG0380017999.01.T01	AT3G11280	50.794	63	31	0	16	78	33	95	7.50e-17	72.4
MsG0380017999.01.T01	AT3G11280	50.794	63	31	0	16	78	33	95	7.50e-17	72.4
MsG0380017999.01.T01	AT5G58900	47.059	68	36	0	14	81	32	99	8.26e-17	72.8
MsG0380017999.01.T01	AT5G08520	49.254	67	34	0	14	80	10	76	3.31e-16	71.2
MsG0380017999.01.T01	AT5G04760	41.791	67	38	1	12	78	2	67	1.63e-13	62.8
MsG0380017999.01.T01	AT1G49010	42.647	68	36	1	14	78	6	73	6.36e-13	62.4
MsG0380017999.01.T01	AT5G01200	43.284	67	37	1	13	78	27	93	1.57e-12	60.8
MsG0380017999.01.T01	AT5G23650	39.189	74	45	0	5	78	2	75	1.21e-11	58.9

Find 16 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCACGTGGATTTGTAATT+TGG	0.099924	3:+103055378	None:intergenic
ACAAGCCTTGTGATTCAATT+TGG	0.187124	3:-103055578	MsG0380017999.01.T01:CDS
GTGGATTTGTAATTTGGAAT+AGG	0.364725	3:+103055384	None:intergenic
AGCACTTGCAAAATTTGATA+AGG	0.422428	3:-103055526	MsG0380017999.01.T01:CDS
CACAAGGCTTGTGTTTGCTA+AGG	0.474591	3:+103055589	None:intergenic
GATCTCAAACACATTGAGTC+TGG	0.498892	3:-103055414	MsG0380017999.01.T01:CDS
GAGACACTATGAGCTACTCT+TGG	0.504426	3:-103055439	MsG0380017999.01.T01:CDS
CTAATTTGCTACACTTCACG+TGG	0.522033	3:+103055365	None:intergenic
ACAAGGCTTGTGTTTGCTAA+GGG	0.544579	3:+103055590	None:intergenic
ACACTATGAGCTACTCTTGG+AGG	0.567606	3:-103055436	MsG0380017999.01.T01:CDS
GTGTTTGCTAAGGGAACTTG+AGG	0.586339	3:+103055599	None:intergenic
AATGTAGCAAAAGCAGTTGG+TGG	0.597235	3:-103055483	MsG0380017999.01.T01:CDS
CAAAATGTAGCAAAAGCAGT+TGG	0.599555	3:-103055486	MsG0380017999.01.T01:CDS
GGAGTCCAAATTGAATCACA+AGG	0.603739	3:+103055573	None:intergenic
ATAAGGATACCCCTGACCGT+TGG	0.605106	3:-103055509	MsG0380017999.01.T01:CDS
AGTTGGTGGTAAATCAGTTG+AGG	0.635333	3:-103055469	MsG0380017999.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCAAACAACTTGTTTTGTTT+TGG	+	Chr3:103055434-103055453	None:intergenic	25.0%
	AGCACTTGCAAAATTTGATA+AGG	-	Chr3:103055457-103055476	MsG0380017999.01.T01:CDS	30.0%
!	GTGGATTTGTAATTTGGAAT+AGG	+	Chr3:103055602-103055621	None:intergenic	30.0%
	CAAAATGTAGCAAAAGCAGT+TGG	-	Chr3:103055497-103055516	MsG0380017999.01.T01:CDS	35.0%
!	ACAAGCCTTGTGATTCAATT+TGG	-	Chr3:103055405-103055424	MsG0380017999.01.T01:CDS	35.0%
!	CTTTTGCTACATTTTGCCAA+CGG	+	Chr3:103055493-103055512	None:intergenic	35.0%
	AATGTAGCAAAAGCAGTTGG+TGG	-	Chr3:103055500-103055519	MsG0380017999.01.T01:CDS	40.0%
	GATCTCAAACACATTGAGTC+TGG	-	Chr3:103055569-103055588	MsG0380017999.01.T01:CDS	40.0%
	GGAGTCCAAATTGAATCACA+AGG	+	Chr3:103055413-103055432	None:intergenic	40.0%
!	ACAAGGCTTGTGTTTGCTAA+GGG	+	Chr3:103055396-103055415	None:intergenic	40.0%
!!	AGTTGGTGGTAAATCAGTTG+AGG	-	Chr3:103055514-103055533	MsG0380017999.01.T01:CDS	40.0%
	ACACTATGAGCTACTCTTGG+AGG	-	Chr3:103055547-103055566	MsG0380017999.01.T01:CDS	45.0%
	GAGACACTATGAGCTACTCT+TGG	-	Chr3:103055544-103055563	MsG0380017999.01.T01:CDS	45.0%
!	CACAAGGCTTGTGTTTGCTA+AGG	+	Chr3:103055397-103055416	None:intergenic	45.0%
!	CTACATTTTGCCAACGGTCA+GGG	+	Chr3:103055487-103055506	None:intergenic	45.0%
!	GTGTTTGCTAAGGGAACTTG+AGG	+	Chr3:103055387-103055406	None:intergenic	45.0%
!	TACATTTTGCCAACGGTCAG+GGG	+	Chr3:103055486-103055505	None:intergenic	45.0%
	ATAAGGATACCCCTGACCGT+TGG	-	Chr3:103055474-103055493	MsG0380017999.01.T01:CDS	50.0%
!	GCTACATTTTGCCAACGGTC+AGG	+	Chr3:103055488-103055507	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	103055380	103055625	103055380	ID=MsG0380017999.01;Name=MsG0380017999.01
Chr3	mRNA	103055380	103055625	103055380	ID=MsG0380017999.01.T01;Parent=MsG0380017999.01;Name=MsG0380017999.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|81
Chr3	exon	103055380	103055625	103055380	ID=MsG0380017999.01.T01:exon:20109;Parent=MsG0380017999.01.T01
Chr3	CDS	103055380	103055625	103055380	ID=MsG0380017999.01.T01:cds;Parent=MsG0380017999.01.T01

Gene Sequence	>MsG0380017999.01.T01 ATGGCCTCAAGTTCCCTTAGCAAACACAAGCCTTGTGATTCAATTTGGACTCCAAAACAAAACAAGTTGTTTGAAAAAGCACTTGCAAAATTTGATAAGGATACCCCTGACCGTTGGCAAAATGTAGCAAAAGCAGTTGGTGGTAAATCAGTTGAGGAAGTTAAGAGACACTATGAGCTACTCTTGGAGGATCTCAAACACATTGAGTCTGGTCATGTTCCTATTCCAAATTACAAATCCACGTGA
Protein sequence	>MsG0380017999.01.T01 MASSSLSKHKPCDSIWTPKQNKLFEKALAKFDKDTPDRWQNVAKAVGGKSVEEVKRHYELLLEDLKHIESGHVPIPNYKST*