AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018918.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018918.01.T01 MTR_4g020070 73.387 124 2 1 49 172 46 138 2.86e-54 177
MsG0480018918.01.T01 MTR_4g020070 73.387 124 2 1 49 172 46 138 9.47e-54 178
MsG0480018918.01.T01 MTR_8g031030 57.258 124 22 1 49 172 46 138 1.18e-38 138
MsG0480018918.01.T01 MTR_4g065003 54.839 124 25 1 49 172 47 139 4.32e-38 135
MsG0480018918.01.T01 MTR_4g065003 54.839 124 25 1 49 172 47 139 1.15e-37 135
MsG0480018918.01.T01 MTR_7g077150 54.032 124 26 1 49 172 46 138 1.99e-36 132
MsG0480018918.01.T01 MTR_8g098370 51.613 124 29 1 49 172 48 140 1.07e-33 124
MsG0480018918.01.T01 MTR_8g098370 51.613 124 29 1 49 172 48 140 1.39e-33 124
MsG0480018918.01.T01 MTR_4g098740 49.194 124 32 1 49 172 50 142 1.82e-31 119
MsG0480018918.01.T01 MTR_5g098970 49.640 139 32 3 35 172 66 167 6.02e-30 114
MsG0480018918.01.T01 MTR_2g097860 44.262 122 24 1 51 172 32 109 3.10e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018918.01.T01 AT3G54030 58.065 124 21 1 49 172 43 135 1.11e-41 146
MsG0480018918.01.T01 AT4G00710 57.258 124 22 1 49 172 45 137 5.16e-39 139
MsG0480018918.01.T01 AT4G00710 57.258 124 22 1 49 172 45 137 5.16e-39 139
MsG0480018918.01.T01 AT1G63500 55.645 124 24 1 49 172 46 138 1.91e-38 137
MsG0480018918.01.T01 AT5G41260 52.419 124 28 1 49 172 46 138 4.25e-37 134
MsG0480018918.01.T01 AT5G59010 53.226 124 27 1 49 172 42 134 4.98e-34 125
MsG0480018918.01.T01 AT3G09240 47.581 124 34 1 49 172 31 123 1.53e-31 119
MsG0480018918.01.T01 AT3G09240 47.581 124 34 1 49 172 46 138 2.21e-31 118
MsG0480018918.01.T01 AT1G01740 50.806 124 30 1 49 172 43 135 7.73e-31 117
MsG0480018918.01.T01 AT1G01740 50.806 124 30 1 49 172 43 135 8.81e-31 117
MsG0480018918.01.T01 AT1G01740 50.806 124 30 1 49 172 43 135 8.81e-31 117
MsG0480018918.01.T01 AT4G35230 50.820 122 29 1 51 172 65 155 2.14e-30 116
MsG0480018918.01.T01 AT5G46570 47.581 124 34 1 49 172 43 135 4.09e-30 115
MsG0480018918.01.T01 AT5G01060 44.715 123 37 1 49 171 50 141 3.98e-28 107
MsG0480018918.01.T01 AT5G01060 44.715 123 37 1 49 171 50 141 2.44e-27 107

Find 36 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGCATCTCTTTCATTGT+AGG 0.272219 4:+11737584 MsG0480018918.01.T01:CDS
TTCCCTTTGTAACCAACATT+TGG 0.342102 4:-11737089 None:intergenic
AACAATGAATTGTCGAGAAT+CGG 0.387156 4:-11737165 None:intergenic
TCGCCAAACGATCACTTCTA+AGG 0.408329 4:-11737490 None:intergenic
GTTCTTGAATCCTCTGACCA+AGG 0.421943 4:+11736762 MsG0480018918.01.T01:CDS
AGATAATATCTTGCATGAAA+TGG 0.426195 4:-11737222 None:intergenic
GCTCTAAATTCTCTTTCTGC+TGG 0.430452 4:+11736715 MsG0480018918.01.T01:CDS
GATCGTTTGGCGAATTTGAT+TGG 0.431219 4:+11737500 MsG0480018918.01.T01:CDS
TTGATTGGTTGTTGCTATGA+AGG 0.442906 4:+11737515 MsG0480018918.01.T01:CDS
GGACCTTAGAAGTGATCGTT+TGG 0.458845 4:+11737487 MsG0480018918.01.T01:CDS
ACAATGAATTGTCGAGAATC+GGG 0.460524 4:-11737164 None:intergenic
CTCCAAATGTTGGTTACAAA+GGG 0.474679 4:+11737087 MsG0480018918.01.T01:CDS
GTACAGTTTGGAAGAATTGA+AGG 0.482014 4:+11737010 MsG0480018918.01.T01:intron
GAATTGAAGGCTGCTACAAA+TGG 0.497274 4:+11737023 MsG0480018918.01.T01:CDS
GCTCCAAATGTTGGTTACAA+AGG 0.503634 4:+11737086 MsG0480018918.01.T01:CDS
AACGTTTCAACAAGTTCGCT+TGG 0.507748 4:+11737141 MsG0480018918.01.T01:CDS
GAACTTGTTGAAACGTTTGA+CGG 0.521430 4:-11737135 None:intergenic
TACAAAGGGAAACTTGAGAA+TGG 0.544684 4:+11737101 MsG0480018918.01.T01:CDS
AGTTTATGCCACATGAACTC+TGG 0.555057 4:+11737561 MsG0480018918.01.T01:CDS
TAGGAAGAATCTAAACAAGT+GGG 0.556675 4:+11737464 MsG0480018918.01.T01:intron
AGAACACAATGAAGTGAAAG+TGG 0.568385 4:-11737198 None:intergenic
CACTGTCCTAGTTCTAATTG+TGG 0.575006 4:+11737256 MsG0480018918.01.T01:CDS
TGCTACAAATGGTTTCTCCT+CGG 0.576389 4:+11737034 MsG0480018918.01.T01:CDS
GTAGGAAGAATCTAAACAAG+TGG 0.580662 4:+11737463 MsG0480018918.01.T01:intron
AACAAAGTAAAACACAACCT+TGG 0.587493 4:-11736779 None:intergenic
CAGAAGTATTGAGATGAGAG+TGG 0.597989 4:-11736740 None:intergenic
GGTGAGAAAGCTCCAAATGT+TGG 0.598384 4:+11737077 MsG0480018918.01.T01:CDS
GAAAACATAGTTTCTGAGCA+TGG 0.608446 4:+11737056 MsG0480018918.01.T01:CDS
TGCAGACCACAATTAGAACT+AGG 0.610858 4:-11737262 None:intergenic
TAAAACACAACCTTGGTCAG+AGG 0.618333 4:-11736772 None:intergenic
ATGCTTAGCCAGAGTTCATG+TGG 0.630751 4:-11737569 None:intergenic
TCATGCAAGATATTATCTTG+CGG 0.634547 4:+11737227 MsG0480018918.01.T01:CDS
GGAAGAATCTAAACAAGTGG+GGG 0.646489 4:+11737466 MsG0480018918.01.T01:intron
AGGAGATGAGAGGCTGCTAG+TGG 0.646691 4:+11737535 MsG0480018918.01.T01:CDS
AGGAAGAATCTAAACAAGTG+GGG 0.655629 4:+11737465 MsG0480018918.01.T01:intron
GTTGCTATGAAGGAGATGAG+AGG 0.745615 4:+11737525 MsG0480018918.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTTATTATTGTAGAAAA+TGG + Chr4:11736949-11736968 MsG0480018918.01.T01:intron 15.0%
!! TAAAAAGTGAATTAATTCAA+TGG - Chr4:11736890-11736909 None:intergenic 15.0%
!! TTTATTATTGTAGAAAATGG+TGG + Chr4:11736952-11736971 MsG0480018918.01.T01:intron 20.0%
!!! AAAATATGTTGTGATTTTGT+AGG + Chr4:11737445-11737464 MsG0480018918.01.T01:intron 20.0%
! AGATAATATCTTGCATGAAA+TGG - Chr4:11737225-11737244 None:intergenic 25.0%
!!! AATTATTATCAGTATCGATG+TGG + Chr4:11736909-11736928 MsG0480018918.01.T01:intron 25.0%
AACAAAGTAAAACACAACCT+TGG - Chr4:11736782-11736801 None:intergenic 30.0%
AACAATGAATTGTCGAGAAT+CGG - Chr4:11737168-11737187 None:intergenic 30.0%
TAGGAAGAATCTAAACAAGT+GGG + Chr4:11737464-11737483 MsG0480018918.01.T01:intron 30.0%
TCATGCAAGATATTATCTTG+CGG + Chr4:11737227-11737246 MsG0480018918.01.T01:CDS 30.0%
TTCAATGGAATTACATGTGT+CGG - Chr4:11736875-11736894 None:intergenic 30.0%
!!! TCCTTCTTATTTTCTTCATG+GGG + Chr4:11736685-11736704 MsG0480018918.01.T01:CDS 30.0%
ACAATGAATTGTCGAGAATC+GGG - Chr4:11737167-11737186 None:intergenic 35.0%
AGAACACAATGAAGTGAAAG+TGG - Chr4:11737201-11737220 None:intergenic 35.0%
AGGAAGAATCTAAACAAGTG+GGG + Chr4:11737465-11737484 MsG0480018918.01.T01:intron 35.0%
ATGCCTTTGATCAGAATTGT+CGG - Chr4:11736837-11736856 None:intergenic 35.0%
CAAACTGTACTCATGAAACT+AGG - Chr4:11737000-11737019 None:intergenic 35.0%
CTAAGCATCTCTTTCATTGT+AGG + Chr4:11737584-11737603 MsG0480018918.01.T01:CDS 35.0%
CTAGTTTCATGAGTACAGTT+TGG + Chr4:11736998-11737017 MsG0480018918.01.T01:intron 35.0%
GAAAACATAGTTTCTGAGCA+TGG + Chr4:11737056-11737075 MsG0480018918.01.T01:CDS 35.0%
GATCACATCGATAATCTACA+TGG - Chr4:11737305-11737324 None:intergenic 35.0%
GCAAGAGTGAAAAAAACTTG+TGG + Chr4:11736974-11736993 MsG0480018918.01.T01:intron 35.0%
GTACAGTTTGGAAGAATTGA+AGG + Chr4:11737010-11737029 MsG0480018918.01.T01:intron 35.0%
GTAGGAAGAATCTAAACAAG+TGG + Chr4:11737463-11737482 MsG0480018918.01.T01:intron 35.0%
TACAAAGGGAAACTTGAGAA+TGG + Chr4:11737101-11737120 MsG0480018918.01.T01:CDS 35.0%
TTCCCTTTGTAACCAACATT+TGG - Chr4:11737092-11737111 None:intergenic 35.0%
! CTCCAAATGTTGGTTACAAA+GGG + Chr4:11737087-11737106 MsG0480018918.01.T01:CDS 35.0%
! TTGATTGGTTGTTGCTATGA+AGG + Chr4:11737515-11737534 MsG0480018918.01.T01:CDS 35.0%
!! GAACTTGTTGAAACGTTTGA+CGG - Chr4:11737138-11737157 None:intergenic 35.0%
!!! CCTTCTTATTTTCTTCATGG+GGG + Chr4:11736686-11736705 MsG0480018918.01.T01:CDS 35.0%
!!! CTTCTTATTTTCTTCATGGG+GGG + Chr4:11736687-11736706 MsG0480018918.01.T01:CDS 35.0%
AACGTTTCAACAAGTTCGCT+TGG + Chr4:11737141-11737160 MsG0480018918.01.T01:CDS 40.0%
ACACCGACAATTCTGATCAA+AGG + Chr4:11736831-11736850 MsG0480018918.01.T01:intron 40.0%
AGTTTATGCCACATGAACTC+TGG + Chr4:11737561-11737580 MsG0480018918.01.T01:CDS 40.0%
CCCCCATGAAGAAAATAAGA+AGG - Chr4:11736689-11736708 None:intergenic 40.0%
GAATTGAAGGCTGCTACAAA+TGG + Chr4:11737023-11737042 MsG0480018918.01.T01:CDS 40.0%
GATCGTTTGGCGAATTTGAT+TGG + Chr4:11737500-11737519 MsG0480018918.01.T01:CDS 40.0%
GGAAGAATCTAAACAAGTGG+GGG + Chr4:11737466-11737485 MsG0480018918.01.T01:intron 40.0%
TAAAACACAACCTTGGTCAG+AGG - Chr4:11736775-11736794 None:intergenic 40.0%
TGCAGACCACAATTAGAACT+AGG - Chr4:11737265-11737284 None:intergenic 40.0%
TGCTACAAATGGTTTCTCCT+CGG + Chr4:11737034-11737053 MsG0480018918.01.T01:CDS 40.0%
! CTCAGAAACTATGTTTTCCG+AGG - Chr4:11737054-11737073 None:intergenic 40.0%
! GCTCCAAATGTTGGTTACAA+AGG + Chr4:11737086-11737105 MsG0480018918.01.T01:CDS 40.0%
! GCTCTAAATTCTCTTTCTGC+TGG + Chr4:11736715-11736734 MsG0480018918.01.T01:CDS 40.0%
!! CACTGTCCTAGTTCTAATTG+TGG + Chr4:11737256-11737275 MsG0480018918.01.T01:CDS 40.0%
!! CAGAAGTATTGAGATGAGAG+TGG - Chr4:11736743-11736762 None:intergenic 40.0%
ATGCTTAGCCAGAGTTCATG+TGG - Chr4:11737572-11737591 None:intergenic 45.0%
CAATCACGTTACAACCGCAA+TGG - Chr4:11737329-11737348 None:intergenic 45.0%
GGACCTTAGAAGTGATCGTT+TGG + Chr4:11737487-11737506 MsG0480018918.01.T01:CDS 45.0%
GGTGAGAAAGCTCCAAATGT+TGG + Chr4:11737077-11737096 MsG0480018918.01.T01:CDS 45.0%
GTTCTTGAATCCTCTGACCA+AGG + Chr4:11736762-11736781 MsG0480018918.01.T01:CDS 45.0%
GTTGCTATGAAGGAGATGAG+AGG + Chr4:11737525-11737544 MsG0480018918.01.T01:CDS 45.0%
TATCGATGTGATCGCCATTG+CGG + Chr4:11737312-11737331 MsG0480018918.01.T01:intron 45.0%
TCGCCAAACGATCACTTCTA+AGG - Chr4:11737493-11737512 None:intergenic 45.0%
!! CAGAATTGTCGGTGTTACAG+CGG - Chr4:11736826-11736845 None:intergenic 45.0%
GCAGCTGCAGCATAAAGACT+TGG - Chr4:11737361-11737380 None:intergenic 50.0%
GCTGCAGCATAAAGACTTGG+AGG - Chr4:11737358-11737377 None:intergenic 50.0%
AGGAGATGAGAGGCTGCTAG+TGG + Chr4:11737535-11737554 MsG0480018918.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 11736685 11737613 11736685 ID=MsG0480018918.01;Name=MsG0480018918.01
Chr4 mRNA 11736685 11737613 11736685 ID=MsG0480018918.01.T01;Parent=MsG0480018918.01;Name=MsG0480018918.01.T01;_AED=0.63;_eAED=0.72;_QI=0|0|0|1|0.5|0.66|3|0|183
Chr4 exon 11736685 11736827 11736685 ID=MsG0480018918.01.T01:exon:7345;Parent=MsG0480018918.01.T01
Chr4 exon 11737016 11737277 11737016 ID=MsG0480018918.01.T01:exon:7346;Parent=MsG0480018918.01.T01
Chr4 exon 11737467 11737613 11737467 ID=MsG0480018918.01.T01:exon:7347;Parent=MsG0480018918.01.T01
Chr4 CDS 11736685 11736827 11736685 ID=MsG0480018918.01.T01:cds;Parent=MsG0480018918.01.T01
Chr4 CDS 11737016 11737277 11737016 ID=MsG0480018918.01.T01:cds;Parent=MsG0480018918.01.T01
Chr4 CDS 11737467 11737613 11737467 ID=MsG0480018918.01.T01:cds;Parent=MsG0480018918.01.T01
Gene Sequence

>MsG0480018918.01.T01

TCCTTCTTATTTTCTTCATGGGGGGTCGTTGCTCTAAATTCTCTTTCTGCTGGTTCCACTCTCATCTCAATACTTCTGTTCTTGAATCCTCTGACCAAGGTTGTGTTTTACTTTGTTATTTTTAGTAGACACATGACACCGCTTTTGGAAGAATTGAAGGCTGCTACAAATGGTTTCTCCTCGGAAAACATAGTTTCTGAGCATGGTGAGAAAGCTCCAAATGTTGGTTACAAAGGGAAACTTGAGAATGGACAGTTGATTGCCGTCAAACGTTTCAACAAGTTCGCTTGGCCCGATTCTCGACAATTCATTGTTTGTTTACTTGCCACTTTCACTTCATTGTGTTCTACCATTTCATGCAAGATATTATCTTGCGGTGCGATCACTGTCCTAGTTCTAATTGTGGAAGAATCTAAACAAGTGGGGGACCTTAGAAGTGATCGTTTGGCGAATTTGATTGGTTGTTGCTATGAAGGAGATGAGAGGCTGCTAGTGGCAGAGTTTATGCCACATGAACTCTGGCTAAGCATCTCTTTCATTGTAGGTTCTTAG

Protein sequence

>MsG0480018918.01.T01

SFLFSSWGVVALNSLSAGSTLISILLFLNPLTKVVFYFVIFSRHMTPLLEELKAATNGFSSENIVSEHGEKAPNVGYKGKLENGQLIAVKRFNKFAWPDSRQFIVCLLATFTSLCSTISCKILSCGAITVLVLIVEESKQVGDLRSDRLANLIGCCYEGDERLLVAEFMPHELWLSISFIVGS*