AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019356.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019356.01.T01 MTR_4g027430 86.056 251 15 1 1 251 1 231 3.93e-158 438
MsG0480019356.01.T01 MTR_2g064690 74.497 149 16 2 105 251 1 129 2.77e-72 217
MsG0480019356.01.T01 MTR_2g064670 96.386 83 3 0 1 83 1 83 3.30e-53 167
MsG0480019356.01.T01 MTR_2g064700 85.870 92 5 1 1 92 1 84 2.91e-48 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019356.01.T01 AT1G12244 57.082 233 77 4 17 246 31 243 3.47e-74 226

Find 30 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGAAAGTGTTACTGTTAA+AGG 0.213689 4:+18993533 None:intergenic
AGAGTATGTAAATTTGAAAA+AGG 0.215824 4:-18994064 MsG0480019356.01.T01:exon
TTCTTCCTCCGAAGCGCGTT+TGG 0.249873 4:+18993647 None:intergenic
TTGAAAATGAAAATAGCTTA+TGG 0.256089 4:+18993840 None:intergenic
TCAAATAAGAGTTTAGATAG+AGG 0.383763 4:-18994020 MsG0480019356.01.T01:exon
TTCTCTCTTGGTGTGGACTT+AGG 0.392088 4:-18993602 MsG0480019356.01.T01:exon
AACTTCCTCCAAACGCGCTT+CGG 0.404160 4:-18993655 MsG0480019356.01.T01:exon
TGGAGAGGTGGGTTCTCTCT+TGG 0.437334 4:-18993614 MsG0480019356.01.T01:exon
CAGAAAGTGTTACTGTTAAA+GGG 0.439382 4:+18993534 None:intergenic
AAGAGTTTCAAGGGTGGCAC+TGG 0.486520 4:+18993770 None:intergenic
GACTTAGGAATGGCTCGTAC+TGG 0.490640 4:-18993587 MsG0480019356.01.T01:exon
AAATGAGTGGAAGAGTTTCA+AGG 0.497687 4:+18993760 None:intergenic
TTCCTCCGAAGCGCGTTTGG+AGG 0.506216 4:+18993650 None:intergenic
AAAGATGCAGAATGGAGAGG+TGG 0.518239 4:-18993626 MsG0480019356.01.T01:exon
TCTTGGTGTGGACTTAGGAA+TGG 0.521326 4:-18993597 MsG0480019356.01.T01:exon
CAAATAAGAGTTTAGATAGA+GGG 0.526887 4:-18994019 MsG0480019356.01.T01:exon
AGGTGGGTTCTCTCTTGGTG+TGG 0.539897 4:-18993609 MsG0480019356.01.T01:exon
CAATTTCTCTCTCTCTAATG+TGG 0.560553 4:-18993814 MsG0480019356.01.T01:exon
TAATGTCTCTATCAACCAGA+GGG 0.563212 4:-18993977 MsG0480019356.01.T01:exon
AATGAGTGGAAGAGTTTCAA+GGG 0.564579 4:+18993761 None:intergenic
TTCCTCCAAACGCGCTTCGG+AGG 0.565039 4:-18993652 MsG0480019356.01.T01:exon
ACTGGCATTGCTCTCAGCAA+AGG 0.577358 4:-18993569 MsG0480019356.01.T01:exon
GGAGGAAGAAAGATGCAGAA+TGG 0.578101 4:-18993634 MsG0480019356.01.T01:exon
AAGAAAGATGCAGAATGGAG+AGG 0.598221 4:-18993629 MsG0480019356.01.T01:exon
AAGATGCAGAATGGAGAGGT+GGG 0.613873 4:-18993625 MsG0480019356.01.T01:exon
ATGTCTCTATCAACCAGAGG+GGG 0.669191 4:-18993975 MsG0480019356.01.T01:exon
ATAATGTCTCTATCAACCAG+AGG 0.674279 4:-18993978 MsG0480019356.01.T01:exon
GAGTGGAAGAGTTTCAAGGG+TGG 0.680073 4:+18993764 None:intergenic
GATTTGAAGGTTGAAATGAG+TGG 0.689513 4:+18993747 None:intergenic
AATGTCTCTATCAACCAGAG+GGG 0.750132 4:-18993976 MsG0480019356.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTACTTCTATAATTTAAA+AGG - Chr4:18990356-18990375 MsG0480019356.01.T01:intron 10.0%
!! TTCTTCATAATTATTTATTA+TGG + Chr4:18993109-18993128 None:intergenic 10.0%
!!! ATTTGAAATATCATATTTTT+AGG - Chr4:18993476-18993495 MsG0480019356.01.T01:intron 10.0%
!!! TAATAAAAAAGAATGTTTTT+GGG - Chr4:18993674-18993693 MsG0480019356.01.T01:exon 10.0%
!!! TTAATAAAAAAGAATGTTTT+TGG - Chr4:18993673-18993692 MsG0480019356.01.T01:exon 10.0%
!!! TTTGAAATATCATATTTTTA+GGG - Chr4:18993477-18993496 MsG0480019356.01.T01:intron 10.0%
!!! TTTTTACAAATTGATAATAA+AGG + Chr4:18991067-18991086 None:intergenic 10.0%
!! AATAAGAAAATGAATAGAAA+AGG - Chr4:18991794-18991813 MsG0480019356.01.T01:intron 15.0%
!! ATAAGAAAATGAATAGAAAA+GGG - Chr4:18991795-18991814 MsG0480019356.01.T01:intron 15.0%
!! CATTATTCTTAATTCTTATA+AGG - Chr4:18992217-18992236 MsG0480019356.01.T01:intron 15.0%
!! TACTATTACATCAAAATAAA+CGG + Chr4:18994115-18994134 None:intergenic 15.0%
!!! AAACAATTTTATATCCAAAA+TGG + Chr4:18993210-18993229 None:intergenic 15.0%
!!! AAATTGATAATAAAGGTAAT+CGG + Chr4:18991060-18991079 None:intergenic 15.0%
!!! TAACATTTTTGAAAAAAATG+AGG - Chr4:18993504-18993523 MsG0480019356.01.T01:intron 15.0%
!!! TACAAATGATAGTTTTTTAT+TGG + Chr4:18990651-18990670 None:intergenic 15.0%
!!! TATATAGTTTAAAACCATTT+TGG - Chr4:18993193-18993212 MsG0480019356.01.T01:intron 15.0%
!!! TATCAATAGATCATTATTTT+AGG + Chr4:18992459-18992478 None:intergenic 15.0%
!!! TGATATTTGAAATGTATATT+GGG - Chr4:18991162-18991181 MsG0480019356.01.T01:intron 15.0%
!!! TTCTATTCATTTTCTTATTT+TGG + Chr4:18991793-18991812 None:intergenic 15.0%
!!! TTGATATTTGAAATGTATAT+TGG - Chr4:18991161-18991180 MsG0480019356.01.T01:intron 15.0%
!!! TTTTGATGTAATAGTAAAAA+TGG - Chr4:18994119-18994138 MsG0480019356.01.T01:exon 15.0%
!! AAAGATAAATTAGAATTCTG+CGG + Chr4:18993013-18993032 None:intergenic 20.0%
!! AAAGTAGATACACAATTATA+AGG + Chr4:18991440-18991459 None:intergenic 20.0%
!! AAGAATTAAGAATAATGACT+TGG + Chr4:18992214-18992233 None:intergenic 20.0%
!! ACGAACAAAAGATAAAATAA+AGG - Chr4:18992045-18992064 MsG0480019356.01.T01:CDS 20.0%
!! AGAGTATGTAAATTTGAAAA+AGG - Chr4:18989600-18989619 MsG0480019356.01.T01:exon 20.0%
!! CATTACAAAACTTCAAATTA+AGG - Chr4:18994054-18994073 MsG0480019356.01.T01:exon 20.0%
!! CTATTATCTTTATACTCTAA+TGG - Chr4:18992387-18992406 MsG0480019356.01.T01:intron 20.0%
!! GAAGATTAATCACTTAATAA+AGG - Chr4:18993911-18993930 MsG0480019356.01.T01:exon 20.0%
!! GAGTATGTAAATTTGAAAAA+GGG - Chr4:18989601-18989620 MsG0480019356.01.T01:exon 20.0%
!! GATACTGATAAAATTGTAAA+CGG + Chr4:18990455-18990474 None:intergenic 20.0%
!! TATAATTGTGTATCTACTTT+AGG - Chr4:18991440-18991459 MsG0480019356.01.T01:intron 20.0%
!! TGAAATTCTGGATATTTATA+GGG - Chr4:18990412-18990431 MsG0480019356.01.T01:intron 20.0%
!! TGAATTAAATTCTATATACG+AGG - Chr4:18990766-18990785 MsG0480019356.01.T01:intron 20.0%
!! TTGAAAATGAAAATAGCTTA+TGG + Chr4:18989827-18989846 None:intergenic 20.0%
!! TTGAATGTAATCAAATATGT+TGG + Chr4:18990338-18990357 None:intergenic 20.0%
!!! AAAACTGGTTCTTATTTATA+AGG - Chr4:18992555-18992574 MsG0480019356.01.T01:intron 20.0%
!!! AAATTTGAAAAAGGGTTTTA+AGG - Chr4:18989609-18989628 MsG0480019356.01.T01:exon 20.0%
!!! ATTTTTGAAAAAAATGAGGA+AGG - Chr4:18993508-18993527 MsG0480019356.01.T01:intron 20.0%
!!! CTTAATTTGAAGTTTTGTAA+TGG + Chr4:18994056-18994075 None:intergenic 20.0%
!!! TTTCTGTTGAAAATTTTGAA+AGG - Chr4:18990172-18990191 MsG0480019356.01.T01:intron 20.0%
!!! TTTTTATGTTTGCGAATTAT+TGG + Chr4:18991953-18991972 None:intergenic 20.0%
!!! TTTTTGAAAAAAATGAGGAA+GGG - Chr4:18993509-18993528 MsG0480019356.01.T01:intron 20.0%
! AAAAACAAACACCAATCAAA+AGG - Chr4:18990833-18990852 MsG0480019356.01.T01:intron 25.0%
! AAATTAGAAAACACGAATGA+TGG + Chr4:18993950-18993969 None:intergenic 25.0%
! AATCCAATTTACTTCAGATT+TGG - Chr4:18991103-18991122 MsG0480019356.01.T01:intron 25.0%
! ATAGATATTGAAGAATAGCA+AGG + Chr4:18992271-18992290 None:intergenic 25.0%
! ATGAAAAGAGAAGACAATTA+TGG - Chr4:18993734-18993753 MsG0480019356.01.T01:exon 25.0%
! CAAATAAGAGTTTAGATAGA+GGG - Chr4:18989645-18989664 MsG0480019356.01.T01:exon 25.0%
! CAACAAAGTTGTAAAATGTT+GGG - Chr4:18990246-18990265 MsG0480019356.01.T01:CDS 25.0%
! CAATCGTTCAGTATTTATAA+AGG + Chr4:18990706-18990725 None:intergenic 25.0%
! CCTATAATTCAAAATCATCT+TGG + Chr4:18991600-18991619 None:intergenic 25.0%
! GAGAAATAATCATCATCTTT+GGG + Chr4:18993887-18993906 None:intergenic 25.0%
! GATGTAATAGTAAAAATGGT+AGG - Chr4:18994123-18994142 MsG0480019356.01.T01:exon 25.0%
! GTAAGTTTAATCATTGGATT+TGG - Chr4:18991335-18991354 MsG0480019356.01.T01:intron 25.0%
! GTGAAATTCTGGATATTTAT+AGG - Chr4:18990411-18990430 MsG0480019356.01.T01:intron 25.0%
! TATATCTACACGATGAATAT+GGG - Chr4:18992784-18992803 MsG0480019356.01.T01:intron 25.0%
! TATTTCAAATACACTTACCA+TGG + Chr4:18993466-18993485 None:intergenic 25.0%
! TCAAATAAGAGTTTAGATAG+AGG - Chr4:18989644-18989663 MsG0480019356.01.T01:exon 25.0%
! TCAACAAAGTTGTAAAATGT+TGG - Chr4:18990245-18990264 MsG0480019356.01.T01:CDS 25.0%
! TCTAAAAACAGCATTAACTT+TGG - Chr4:18989983-18990002 MsG0480019356.01.T01:intron 25.0%
! TCTGATGCTTATTTAGTATT+TGG - Chr4:18992642-18992661 MsG0480019356.01.T01:intron 25.0%
! TGAGAAATAATCATCATCTT+TGG + Chr4:18993888-18993907 None:intergenic 25.0%
! TGGTATGTAAGTTTAATCAT+TGG - Chr4:18991329-18991348 MsG0480019356.01.T01:intron 25.0%
!! ACAAACTTGATTTTTCCTTT+CGG + Chr4:18992620-18992639 None:intergenic 25.0%
!! AGTCCTTTAAGAATTCAAAA+TGG - Chr4:18992413-18992432 MsG0480019356.01.T01:intron 25.0%
!! CCAAGATGATTTTGAATTAT+AGG - Chr4:18991597-18991616 MsG0480019356.01.T01:intron 25.0%
!! GAACCATTTTGAATTCTTAA+AGG + Chr4:18992419-18992438 None:intergenic 25.0%
!! GATTTGAATTTTGTTCCTTT+AGG - Chr4:18993589-18993608 MsG0480019356.01.T01:exon 25.0%
!! TAATTGTCTTCTCTTTTCAT+TGG + Chr4:18993734-18993753 None:intergenic 25.0%
!! TGAATTTTTGCATGATTTGA+AGG + Chr4:18989933-18989952 None:intergenic 25.0%
!! TGCAATTTTATCCTTTTGAT+TGG + Chr4:18990847-18990866 None:intergenic 25.0%
!! TGGTAAAACACTTTTCTTTT+AGG + Chr4:18993234-18993253 None:intergenic 25.0%
!!! AAATCACATTTTGACATGTT+TGG + Chr4:18990601-18990620 None:intergenic 25.0%
!!! ATTTCAAGTAACATTTTGTG+TGG - Chr4:18993370-18993389 MsG0480019356.01.T01:intron 25.0%
!!! TAGATCATTATTTTAGGATG+TGG + Chr4:18992453-18992472 None:intergenic 25.0%
!!! TAGTGGTTGATGAAATAATT+CGG + Chr4:18990919-18990938 None:intergenic 25.0%
!!! TGAAAGTGCTTTTTTTGTTA+CGG - Chr4:18990381-18990400 MsG0480019356.01.T01:intron 25.0%
AAAGATCAAAAAGAGGACTA+TGG + Chr4:18990986-18991005 None:intergenic 30.0%
AAATAATCATCATCTTTGGG+AGG + Chr4:18993884-18993903 None:intergenic 30.0%
AACTTCAAATTAAGGATGTG+TGG - Chr4:18994062-18994081 MsG0480019356.01.T01:exon 30.0%
AATCATCTTGGTTATCTCTA+AGG + Chr4:18991588-18991607 None:intergenic 30.0%
ACACGCTTAAAATTCTTTCA+GGG - Chr4:18993398-18993417 MsG0480019356.01.T01:intron 30.0%
ACAGTTCATATACTACATGA+AGG - Chr4:18992912-18992931 MsG0480019356.01.T01:intron 30.0%
ACTTCAAATTAAGGATGTGT+GGG - Chr4:18994063-18994082 MsG0480019356.01.T01:exon 30.0%
AGTTATAAAACGACACTTTG+AGG + Chr4:18992990-18993009 None:intergenic 30.0%
ATATTAGGTTCTATGTCACT+GGG - Chr4:18991234-18991253 MsG0480019356.01.T01:intron 30.0%
ATGAATAGAAAAGGGATGAA+TGG - Chr4:18991803-18991822 MsG0480019356.01.T01:intron 30.0%
ATTGGGTAAAGATCAAAAAG+AGG + Chr4:18990993-18991012 None:intergenic 30.0%
CAGTTCATATACTACATGAA+GGG - Chr4:18992913-18992932 MsG0480019356.01.T01:intron 30.0%
CATACTAAACAACAAAGACA+CGG + Chr4:18991365-18991384 None:intergenic 30.0%
GAAGCTGATGAGTTTATAAT+TGG - Chr4:18991619-18991638 MsG0480019356.01.T01:intron 30.0%
GTATATCTACACGATGAATA+TGG - Chr4:18992783-18992802 MsG0480019356.01.T01:intron 30.0%
TAAAGTTTCAGCATGTTTAG+TGG + Chr4:18990936-18990955 None:intergenic 30.0%
TAGAGTTCTTAAAGCAAATG+CGG + Chr4:18992370-18992389 None:intergenic 30.0%
TGTGGGAAAAAAATGTCAAA+AGG - Chr4:18994080-18994099 MsG0480019356.01.T01:exon 30.0%
TGTTAATCGACACAATGATT+TGG - Chr4:18994017-18994036 MsG0480019356.01.T01:exon 30.0%
TGTTATCTCATTCCATCTTA+TGG - Chr4:18990568-18990587 MsG0480019356.01.T01:intron 30.0%
TTATGTTTGCGAATTATTGG+AGG + Chr4:18991950-18991969 None:intergenic 30.0%
TTATTTATTATGGACCATGC+AGG + Chr4:18993099-18993118 None:intergenic 30.0%
TTGTCAACACTACATTACAA+TGG + Chr4:18991740-18991759 None:intergenic 30.0%
TTTGAGGAAAAGATCCAATT+AGG + Chr4:18992974-18992993 None:intergenic 30.0%
! ACGGATTAAAAGTGAAATTC+TGG - Chr4:18990400-18990419 MsG0480019356.01.T01:intron 30.0%
! ATTTTACAATGGTCTCTAAG+GGG - Chr4:18993567-18993586 MsG0480019356.01.T01:exon 30.0%
! CATTTTACAATGGTCTCTAA+GGG - Chr4:18993566-18993585 MsG0480019356.01.T01:exon 30.0%
! CGTTAATGACCATTTTACAA+TGG - Chr4:18993556-18993575 MsG0480019356.01.T01:exon 30.0%
! GAATTATTGGAGGTATATCT+CGG + Chr4:18991940-18991959 None:intergenic 30.0%
! TCTAATTTCAAGTATGCGTT+TGG - Chr4:18993962-18993981 MsG0480019356.01.T01:exon 30.0%
! TTATTTCTCGCACTTAATAG+AGG - Chr4:18992680-18992699 MsG0480019356.01.T01:intron 30.0%
!! ACATTTTGACATGTTTGGAT+TGG + Chr4:18990596-18990615 None:intergenic 30.0%
!! CAGAAAGTGTTACTGTTAAA+GGG + Chr4:18990133-18990152 None:intergenic 30.0%
!! GAAGAAGTATGCATGATTTT+GGG - Chr4:18993123-18993142 MsG0480019356.01.T01:intron 30.0%
!! GCAATTTGTTACTCTTAGTT+TGG - Chr4:18990204-18990223 MsG0480019356.01.T01:intron 30.0%
!! TATGTTGGTGTCATGTTATT+CGG + Chr4:18990323-18990342 None:intergenic 30.0%
!! TGAAGAAGTATGCATGATTT+TGG - Chr4:18993122-18993141 MsG0480019356.01.T01:intron 30.0%
!!! AACCAGTTGTGTTTTTAAGT+CGG + Chr4:18992582-18992601 None:intergenic 30.0%
!!! GATTTCTAGCTTGTTTTTCA+TGG + Chr4:18990877-18990896 None:intergenic 30.0%
!!! TGTTTGTTTTTGCTTCTGTT+TGG + Chr4:18990824-18990843 None:intergenic 30.0%
AAAGATCCAATTAGGAGGTA+AGG + Chr4:18992966-18992985 None:intergenic 35.0%
AAATGAGTGGAAGAGTTTCA+AGG + Chr4:18989907-18989926 None:intergenic 35.0%
AAGTTGTAAAATGTTGGGAG+AGG - Chr4:18990251-18990270 MsG0480019356.01.T01:intron 35.0%
AATGAGTGGAAGAGTTTCAA+GGG + Chr4:18989906-18989925 None:intergenic 35.0%
ACATACTACACAACAACACA+TGG + Chr4:18991267-18991286 None:intergenic 35.0%
ACTTTGATCTCGTAACCTAA+AGG + Chr4:18993607-18993626 None:intergenic 35.0%
AGATGAGATAAGAACCTCTA+TGG + Chr4:18992329-18992348 None:intergenic 35.0%
AGTTGTAAAATGTTGGGAGA+GGG - Chr4:18990252-18990271 MsG0480019356.01.T01:intron 35.0%
ATAATGTCTCTATCAACCAG+AGG - Chr4:18989686-18989705 MsG0480019356.01.T01:exon 35.0%
ATGTTACCTTACCTCCTAAT+TGG - Chr4:18992957-18992976 MsG0480019356.01.T01:intron 35.0%
ATTGTATCCTTCAATGCTGT+CGG - Chr4:18992873-18992892 MsG0480019356.01.T01:intron 35.0%
CAATTTCTCTCTCTCTAATG+TGG - Chr4:18989850-18989869 MsG0480019356.01.T01:CDS 35.0%
CACGCTTAAAATTCTTTCAG+GGG - Chr4:18993399-18993418 MsG0480019356.01.T01:intron 35.0%
CATATTAGGTTCTATGTCAC+TGG - Chr4:18991233-18991252 MsG0480019356.01.T01:intron 35.0%
CCTTAGAGACCATTGTAAAA+TGG + Chr4:18993568-18993587 None:intergenic 35.0%
CCTTGTAGTTCAAGATTCTT+GGG + Chr4:18993845-18993864 None:intergenic 35.0%
CTACAAGGAAAACTTAGAAG+TGG - Chr4:18993857-18993876 MsG0480019356.01.T01:exon 35.0%
GACACGCTTAAAATTCTTTC+AGG - Chr4:18993397-18993416 MsG0480019356.01.T01:intron 35.0%
GACATAGAACCTAATATGCA+AGG + Chr4:18991231-18991250 None:intergenic 35.0%
GATTTGAAGGTTGAAATGAG+TGG + Chr4:18989920-18989939 None:intergenic 35.0%
GTCATTCAACCTTGCATATT+AGG - Chr4:18991219-18991238 MsG0480019356.01.T01:intron 35.0%
GTGTGCTTATTAGTGATGTT+AGG - Chr4:18991306-18991325 MsG0480019356.01.T01:intron 35.0%
TAATGTCTCTATCAACCAGA+GGG - Chr4:18989687-18989706 MsG0480019356.01.T01:exon 35.0%
TATCTCAACTCTAACTTCAG+AGG - Chr4:18992753-18992772 MsG0480019356.01.T01:intron 35.0%
TCATAGCTCAGTGATATGTT+TGG - Chr4:18990473-18990492 MsG0480019356.01.T01:intron 35.0%
TCCTTGTAGTTCAAGATTCT+TGG + Chr4:18993846-18993865 None:intergenic 35.0%
TGCTTATTAGTGATGTTAGG+TGG - Chr4:18991309-18991328 MsG0480019356.01.T01:intron 35.0%
TTTCATTGGCACTTCAAAAC+AGG + Chr4:18993720-18993739 None:intergenic 35.0%
! AACATAGTCCGTTCTTTTAC+TGG - Chr4:18991682-18991701 MsG0480019356.01.T01:intron 35.0%
! CCATTTTACAATGGTCTCTA+AGG - Chr4:18993565-18993584 MsG0480019356.01.T01:exon 35.0%
! GAATAGGAATTCGGTGAATT+GGG + Chr4:18991010-18991029 None:intergenic 35.0%
! GGTTCTTATCTCATCTTTTG+TGG - Chr4:18992333-18992352 MsG0480019356.01.T01:intron 35.0%
! TACTCCTATGATGGAACTTA+GGG - Chr4:18992532-18992551 MsG0480019356.01.T01:intron 35.0%
! TGAATAGGAATTCGGTGAAT+TGG + Chr4:18991011-18991030 None:intergenic 35.0%
! TTACTCCTATGATGGAACTT+AGG - Chr4:18992531-18992550 MsG0480019356.01.T01:intron 35.0%
! TTTTCCCTAAGTTCCATCAT+AGG + Chr4:18992539-18992558 None:intergenic 35.0%
!! AAGTACTTTGAGACTGTTTG+AGG + Chr4:18992163-18992182 None:intergenic 35.0%
!! AATGTGTTCGAGCTTTGAAT+AGG + Chr4:18991026-18991045 None:intergenic 35.0%
!! ATGTTTGGATTGGCAGTATA+AGG - Chr4:18990488-18990507 MsG0480019356.01.T01:intron 35.0%
!! GCAAACATAAAAAGTGCTCT+GGG - Chr4:18991960-18991979 MsG0480019356.01.T01:CDS 35.0%
!! GCAGAAAGTGTTACTGTTAA+AGG + Chr4:18990134-18990153 None:intergenic 35.0%
!! TCGAGCTTTGAATAGGAATT+CGG + Chr4:18991019-18991038 None:intergenic 35.0%
!! TTTGGATTGGTACCATAAGA+TGG + Chr4:18990583-18990602 None:intergenic 35.0%
AAGAAAGATGCAGAATGGAG+AGG - Chr4:18990035-18990054 MsG0480019356.01.T01:intron 40.0%
AATGAACATCGCAGAAGAAG+AGG - Chr4:18991500-18991519 MsG0480019356.01.T01:intron 40.0%
AATGTCTCTATCAACCAGAG+GGG - Chr4:18989688-18989707 MsG0480019356.01.T01:exon 40.0%
ACTAGTGATCACTGATGAGA+GGG + Chr4:18993258-18993277 None:intergenic 40.0%
AGGTTACGAGATCAAAGTCT+TGG - Chr4:18993609-18993628 MsG0480019356.01.T01:exon 40.0%
CAAGCCTTCCAGTAAAAGAA+CGG + Chr4:18991693-18991712 None:intergenic 40.0%
CCCAAGAATCTTGAACTACA+AGG - Chr4:18993842-18993861 MsG0480019356.01.T01:exon 40.0%
CTTATTTCCATGAGCATTCC+AGG + Chr4:18992107-18992126 None:intergenic 40.0%
CTTCTGCGATGTTCATTATC+CGG + Chr4:18991497-18991516 None:intergenic 40.0%
GAGAGATATTTCTCCACATC+AGG - Chr4:18993797-18993816 MsG0480019356.01.T01:exon 40.0%
GAGGAAAAGATCCAATTAGG+AGG + Chr4:18992971-18992990 None:intergenic 40.0%
GCTCAGTGATATGTTTGGAT+TGG - Chr4:18990478-18990497 MsG0480019356.01.T01:intron 40.0%
GTTGTAAAATGTTGGGAGAG+GGG - Chr4:18990253-18990272 MsG0480019356.01.T01:intron 40.0%
GTTTGGAAGTTGCGAGAAAA+TGG - Chr4:18993979-18993998 MsG0480019356.01.T01:exon 40.0%
TAACGACTCTGATCTGTGTT+AGG + Chr4:18991194-18991213 None:intergenic 40.0%
TCAACTCTAACTTCAGAGGT+TGG - Chr4:18992757-18992776 MsG0480019356.01.T01:intron 40.0%
TCCTTTCCATCACAAGACTT+TGG + Chr4:18991649-18991668 None:intergenic 40.0%
TGATGGAACTTAGGGAAAAC+TGG - Chr4:18992540-18992559 MsG0480019356.01.T01:intron 40.0%
TGCAGAAATTGAAACCTGCA+TGG - Chr4:18993082-18993101 MsG0480019356.01.T01:intron 40.0%
TGGAATAGAGTCGAGTGAAA+TGG - Chr4:18990511-18990530 MsG0480019356.01.T01:intron 40.0%
TTCTCGCACTTAATAGAGGT+TGG - Chr4:18992684-18992703 MsG0480019356.01.T01:intron 40.0%
TTGTAAAATGTTGGGAGAGG+GGG - Chr4:18990254-18990273 MsG0480019356.01.T01:intron 40.0%
! ACTCTATCGTGTCGATAGAA+TGG + Chr4:18993304-18993323 None:intergenic 40.0%
! GACCGACTTAAAAACACAAC+TGG - Chr4:18992577-18992596 MsG0480019356.01.T01:intron 40.0%
! GCGCCAAATCTGAAGTAAAT+TGG + Chr4:18991109-18991128 None:intergenic 40.0%
! TAGTCCGTTCTTTTACTGGA+AGG - Chr4:18991686-18991705 MsG0480019356.01.T01:intron 40.0%
! TCATCAGTGATCACTAGTCT+CGG - Chr4:18993260-18993279 MsG0480019356.01.T01:intron 40.0%
! TCCAAAGTCTTGTGATGGAA+AGG - Chr4:18991645-18991664 MsG0480019356.01.T01:intron 40.0%
! TTCAGTTTTCTGACCTGATG+TGG + Chr4:18993813-18993832 None:intergenic 40.0%
!! CGCAAACATAAAAAGTGCTC+TGG - Chr4:18991959-18991978 MsG0480019356.01.T01:CDS 40.0%
!! GTTGTTTTCATGAGCTATCC+TGG - Chr4:18992086-18992105 MsG0480019356.01.T01:intron 40.0%
!! TTTGGATTGGCAGTATAAGG+TGG - Chr4:18990491-18990510 MsG0480019356.01.T01:intron 40.0%
!!! CTTTAGGTTTTGAAACTGCG+AGG - Chr4:18991456-18991475 MsG0480019356.01.T01:intron 40.0%
AAAGATGCAGAATGGAGAGG+TGG - Chr4:18990038-18990057 MsG0480019356.01.T01:intron 45.0%
AAGATGCAGAATGGAGAGGT+GGG - Chr4:18990039-18990058 MsG0480019356.01.T01:intron 45.0%
ACAAGAACCATCCTTACACC+TGG - Chr4:18992495-18992514 MsG0480019356.01.T01:intron 45.0%
ATGTCTCTATCAACCAGAGG+GGG - Chr4:18989689-18989708 MsG0480019356.01.T01:exon 45.0%
ATTCTTTCAGGGGCTTGAGT+AGG - Chr4:18993409-18993428 MsG0480019356.01.T01:intron 45.0%
CATGTCGACCATTCGATCTA+TGG + Chr4:18992823-18992842 None:intergenic 45.0%
CCTCGAAAATGAGTAGAGTG+AGG - Chr4:18991128-18991147 MsG0480019356.01.T01:intron 45.0%
GACTAGTGATCACTGATGAG+AGG + Chr4:18993259-18993278 None:intergenic 45.0%
GATGCTCTTTCGTTACCGAA+AGG - Chr4:18992602-18992621 MsG0480019356.01.T01:intron 45.0%
GGAGGAAGAAAGATGCAGAA+TGG - Chr4:18990030-18990049 MsG0480019356.01.T01:intron 45.0%
TACACTTACCATGGCAGCAT+AGG + Chr4:18993457-18993476 None:intergenic 45.0%
TGTAAAATGTTGGGAGAGGG+GGG - Chr4:18990255-18990274 MsG0480019356.01.T01:intron 45.0%
TTGCATTAGTGGGATACCGA+AGG - Chr4:18990279-18990298 MsG0480019356.01.T01:intron 45.0%
! AGGCATCTTGTTTCTTCTGC+TGG + Chr4:18993437-18993456 None:intergenic 45.0%
! CCTCACTCTACTCATTTTCG+AGG + Chr4:18991131-18991150 None:intergenic 45.0%
! GACATAGAGCATCTCCATAG+AGG - Chr4:18992312-18992331 MsG0480019356.01.T01:intron 45.0%
! GGACTTCCAAAGTCTTGTGA+TGG - Chr4:18991640-18991659 MsG0480019356.01.T01:intron 45.0%
! GGCATCTTGTTTCTTCTGCT+GGG + Chr4:18993436-18993455 None:intergenic 45.0%
! TCCAAAACCGACAGCATTGA+AGG + Chr4:18992883-18992902 None:intergenic 45.0%
! TCTTGGTGTGGACTTAGGAA+TGG - Chr4:18990067-18990086 MsG0480019356.01.T01:intron 45.0%
! TTCTCTCTTGGTGTGGACTT+AGG - Chr4:18990062-18990081 MsG0480019356.01.T01:intron 45.0%
!!! GCTGTCGGTTTTGGATGAAA+TGG - Chr4:18992888-18992907 MsG0480019356.01.T01:intron 45.0%
!!! TCCTTCAATGCTGTCGGTTT+TGG - Chr4:18992879-18992898 MsG0480019356.01.T01:intron 45.0%
AACTTCCTCCAAACGCGCTT+CGG - Chr4:18990009-18990028 MsG0480019356.01.T01:intron 50.0%
AAGAGTTTCAAGGGTGGCAC+TGG + Chr4:18989897-18989916 None:intergenic 50.0%
ACAAGATGCCTATGCTGCCA+TGG - Chr4:18993446-18993465 MsG0480019356.01.T01:intron 50.0%
ACATCGCAGAAGAAGAGGTC+TGG - Chr4:18991505-18991524 MsG0480019356.01.T01:intron 50.0%
ACTGCGAGGACAGAAACTTG+AGG - Chr4:18991470-18991489 MsG0480019356.01.T01:intron 50.0%
ACTGGCATTGCTCTCAGCAA+AGG - Chr4:18990095-18990114 MsG0480019356.01.T01:intron 50.0%
AGAGGGGGGATTGCATTAGT+GGG - Chr4:18990269-18990288 MsG0480019356.01.T01:intron 50.0%
ATCAGCCGCCATAGATCGAA+TGG - Chr4:18992812-18992831 MsG0480019356.01.T01:intron 50.0%
GACTTAGGAATGGCTCGTAC+TGG - Chr4:18990077-18990096 MsG0480019356.01.T01:intron 50.0%
GAGCTATCCTGGAATGCTCA+TGG - Chr4:18992097-18992116 MsG0480019356.01.T01:intron 50.0%
GAGGACAGAAACTTGAGGTC+CGG - Chr4:18991475-18991494 MsG0480019356.01.T01:intron 50.0%
GAGTGGAAGAGTTTCAAGGG+TGG + Chr4:18989903-18989922 None:intergenic 50.0%
GCGCAAGCTTACTCCTATGA+TGG - Chr4:18992523-18992542 MsG0480019356.01.T01:intron 50.0%
GTCGACCATTCGATCTATGG+CGG + Chr4:18992820-18992839 None:intergenic 50.0%
GTGATCACTGATGAGAGGGT+TGG + Chr4:18993254-18993273 None:intergenic 50.0%
TCAGACACCAAACACGCCTT+CGG + Chr4:18990298-18990317 None:intergenic 50.0%
TTGCTAATCGAGCTGCTGAG+AGG - Chr4:18991710-18991729 MsG0480019356.01.T01:intron 50.0%
! AAAGTGCTCTGGGCTCTTTC+TGG - Chr4:18991970-18991989 MsG0480019356.01.T01:CDS 50.0%
! GGCACTGAGTTGACATCTCA+TGG - Chr4:18993630-18993649 MsG0480019356.01.T01:exon 50.0%
! TCAGTGATCACTAGTCTCGG+AGG - Chr4:18993263-18993282 MsG0480019356.01.T01:intron 50.0%
ACCAGAGGGGGACAACTCAA+TGG - Chr4:18989701-18989720 MsG0480019356.01.T01:exon 55.0%
AGTAAGCTTGCGCAGATGCC+AGG + Chr4:18992516-18992535 None:intergenic 55.0%
GAGAGGGGGGATTGCATTAG+TGG - Chr4:18990268-18990287 MsG0480019356.01.T01:intron 55.0%
GCAGATGCCAGGTGTAAGGA+TGG + Chr4:18992505-18992524 None:intergenic 55.0%
TTCTTCCTCCGAAGCGCGTT+TGG + Chr4:18990020-18990039 None:intergenic 55.0%
TTGCGCAGATGCCAGGTGTA+AGG + Chr4:18992509-18992528 None:intergenic 55.0%
! ACCATTGAGTTGTCCCCCTC+TGG + Chr4:18989705-18989724 None:intergenic 55.0%
! AGGTGGGTTCTCTCTTGGTG+TGG - Chr4:18990055-18990074 MsG0480019356.01.T01:intron 55.0%
! TGGAGAGGTGGGTTCTCTCT+TGG - Chr4:18990050-18990069 MsG0480019356.01.T01:intron 55.0%
! TGGGATACCGAAGGCGTGTT+TGG - Chr4:18990288-18990307 MsG0480019356.01.T01:intron 55.0%
TTCCTCCAAACGCGCTTCGG+AGG - Chr4:18990012-18990031 MsG0480019356.01.T01:intron 60.0%
TTCCTCCGAAGCGCGTTTGG+AGG + Chr4:18990017-18990036 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 18989536 18994150 18989536 ID=MsG0480019356.01;
Chr4 mRNA 18989536 18994150 18989536 ID=MsG0480019356.01.T01;Parent=MsG0480019356.01
Chr4 exon 18989536 18989898 18989536 ID=MsG0480019356.01.T01.exon;Parent=MsG0480019356.01.T01
Chr4 CDS 18989767 18989898 18989767 ID=MsG0480019356.01.T01.cds;Parent=MsG0480019356.01.T01
Chr4 CDS 18990219 18990267 18990219 ID=MsG0480019356.01.T01.cds;Parent=MsG0480019356.01.T01
Chr4 exon 18990219 18990267 18990219 ID=MsG0480019356.01.T01.exon;Parent=MsG0480019356.01.T01
Chr4 CDS 18990693 18990752 18990693 ID=MsG0480019356.01.T01.cds;Parent=MsG0480019356.01.T01
Chr4 exon 18990693 18990752 18990693 ID=MsG0480019356.01.T01.exon;Parent=MsG0480019356.01.T01
Chr4 CDS 18990845 18990913 18990845 ID=MsG0480019356.01.T01.cds;Parent=MsG0480019356.01.T01
Chr4 exon 18990845 18990913 18990845 ID=MsG0480019356.01.T01.exon;Parent=MsG0480019356.01.T01
Chr4 CDS 18991955 18992067 18991955 ID=MsG0480019356.01.T01.cds;Parent=MsG0480019356.01.T01
Chr4 exon 18991955 18992067 18991955 ID=MsG0480019356.01.T01.exon;Parent=MsG0480019356.01.T01
Chr4 CDS 18992165 18992224 18992165 ID=MsG0480019356.01.T01.cds;Parent=MsG0480019356.01.T01
Chr4 exon 18992165 18992224 18992165 ID=MsG0480019356.01.T01.exon;Parent=MsG0480019356.01.T01
Chr4 CDS 18993547 18993819 18993547 ID=MsG0480019356.01.T01.cds;Parent=MsG0480019356.01.T01
Chr4 exon 18993547 18994150 18993547 ID=MsG0480019356.01.T01.exon;Parent=MsG0480019356.01.T01
Gene Sequence

>MsG0480019356.01.T01

ATGTGGACTCAGCAGCAACTTGTACAACCAGTGCCACCCTTGAAACTCTTCCACTCATTTCAACCTTCAAATCATGCAAAAATTCAATCTTTATCAAAATTAAATTCGACGAACAATCTAAAAACAGCATTAACTTTGGAAGAACTTCCTCCAAACGCGCTTCGGAGGAAGAAAGATGCAGAATGGAGAGGTGGGTTCTCTCTTGGTGTGGACTTAGGAATGGCTCGTACTGGCATTGCTCTCAGCAAAGGCTTCACTTTTCGCCCTTTAACAGTTTTGAAACTGCGAGGACAGAAACTTGAGGTCCGGATAATGAACATCGCAGAAGAAGAGGAAGCTGATGAGTTTATAATTGGACTTCCAAAGTCTTGTGATGGAAAGGAAACGATACAGTCAAACATAGTCCGTTCTTTTACTGGAAGGCTTGCTAATCGAGCTGCTGAGAGAGGTTGGAGAGTATATCTACACGATGAATATGGGACGACATCAGCCGCCATAGATCGAATGGTCGACATGGAAGCTGTTAGAGAAAAAAACTATGTTACCTTACCTCCTAATTGGATCTTTTCCTCAAAGGGCTTGAGTAGGTCCCAGCAGAAGAAACAAGATGCCTATGCTGCCATGATGTTACTTGAGAGATATTTCTCCACATCAGGTCAGAAAACTGAACTTGTTGTGCCCAAGAATCTTGAACTACAAGGAAAACTTAGAAGTGGTCCTCCCAAAGATGATGATTATTTCTCAGACGAAGATTAA
Protein sequence

>MsG0480019356.01.T01

MWTQQQLVQPVPPLKLFHSFQPSNHAKIQSLSKLNSTNNLKTALTLEELPPNALRRKKDAEWRGGFSLGVDLGMARTGIALSKGFTFRPLTVLKLRGQKLEVRIMNIAEEEEADEFIIGLPKSCDGKETIQSNIVRSFTGRLANRAAERGWRVYLHDEYGTTSAAIDRMVDMEAVREKNYVTLPPNWIFSSKGLSRSQQKKQDAYAAMMLLERYFSTSGQKTELVVPKNLELQGKLRSGPPKDDDYFSDED*