AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004276.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004276.01.T01 MTR_1g076310 84.783 92 14 0 14 105 34 125 2.18e-51 158
MsG0180004276.01.T01 MTR_2g039750 47.945 73 31 1 13 78 33 105 9.98e-16 67.8
MsG0180004276.01.T01 MTR_5g014070 47.945 73 31 1 13 78 33 105 1.05e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004276.01.T01 AT5G56940 44.444 72 33 1 13 77 33 104 1.92e-15 67.4
MsG0180004276.01.T01 AT4G34620 50.000 50 25 0 13 62 33 82 2.98e-14 63.5

Find 37 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATTCACAGTAACAAAT+AGG 0.272085 1:+75923456 None:intergenic
TGGATGGCTGCTCTTCGTTC+GGG 0.277399 1:+75923073 None:intergenic
TTGGATGGCTGCTCTTCGTT+CGG 0.292007 1:+75923072 None:intergenic
ACCATCAACTTCTTTGTCAT+TGG 0.341893 1:+75923053 None:intergenic
TCTTCGTTCGGGGTATCAAC+AGG 0.369294 1:+75923084 None:intergenic
AATTGCAATGTATACCAGTT+TGG 0.380322 1:-75923440 MsG0180004276.01.T01:CDS
GGGAATAACAGCCTTATGTT+TGG 0.392068 1:-75922890 MsG0180004276.01.T01:three_prime_UTR
CCCTCCTTCTCATCCCAAAC+TGG 0.413028 1:+75923426 None:intergenic
ATTGCAATGTATACCAGTTT+GGG 0.434755 1:-75923439 MsG0180004276.01.T01:CDS
GCAATTCACAGTAACAAATA+GGG 0.438622 1:+75923457 None:intergenic
TCCAATGACAAAGAAGTTGA+TGG 0.440759 1:-75923054 MsG0180004276.01.T01:intron
TTTAGGTATTGGCTTTCTGT+TGG 0.451329 1:-75923192 MsG0180004276.01.T01:intron
AAAATAAATAAGTAAACAAC+AGG 0.456386 1:+75922661 None:intergenic
AAACGGAAAGGTGGACTGTC+TGG 0.470726 1:-75923114 MsG0180004276.01.T01:CDS
TCAACTTCTTTGTCATTGGA+TGG 0.482393 1:+75923057 None:intergenic
AGCCTTATGTTTGGAGAAGG+TGG 0.488363 1:-75922881 MsG0180004276.01.T01:three_prime_UTR
AGGCAGGTTTACAAATGAAA+CGG 0.502953 1:-75923131 MsG0180004276.01.T01:CDS
ACCCACCTTCTCCAAACATA+AGG 0.511336 1:+75922879 None:intergenic
GCAGAAGCTGTACTTTCTAT+CGG 0.514830 1:-75922930 MsG0180004276.01.T01:CDS
GCCTTATGTTTGGAGAAGGT+GGG 0.530520 1:-75922880 MsG0180004276.01.T01:three_prime_UTR
AGACTCTCCACAGGTTCTGA+AGG 0.549004 1:+75923162 None:intergenic
AACAGCCTTATGTTTGGAGA+AGG 0.551470 1:-75922884 MsG0180004276.01.T01:three_prime_UTR
ACCAGTTTGGGATGAGAAGG+AGG 0.553779 1:-75923427 MsG0180004276.01.T01:CDS
TATACCAGTTTGGGATGAGA+AGG 0.569595 1:-75923430 MsG0180004276.01.T01:CDS
TTGTTAACTGCTTGATCATG+AGG 0.574436 1:-75922702 MsG0180004276.01.T01:three_prime_UTR
TGCTCAACCTTCAGAACCTG+TGG 0.580737 1:-75923169 MsG0180004276.01.T01:CDS
ATGCATACATGACATGGTAG+AGG 0.582753 1:+75922765 None:intergenic
CGGCTTACAAGTTTAACTGA+GGG 0.587865 1:-75922910 MsG0180004276.01.T01:exon
TTGAATAAAAGACTCTCCAC+AGG 0.595461 1:+75923153 None:intergenic
GGATGGCTGCTCTTCGTTCG+GGG 0.597237 1:+75923074 None:intergenic
CTTCGTTCGGGGTATCAACA+GGG 0.597733 1:+75923085 None:intergenic
AAGTTGACGATTTGCAATGG+AGG 0.617421 1:+75922840 None:intergenic
TCAAGCATGCATACATGACA+TGG 0.632655 1:+75922759 None:intergenic
CCAGTTTGGGATGAGAAGGA+GGG 0.633963 1:-75923426 MsG0180004276.01.T01:intron
TCGGCTTACAAGTTTAACTG+AGG 0.642616 1:-75922911 MsG0180004276.01.T01:exon
TTACAAATGAAACGGAAAGG+TGG 0.647965 1:-75923123 MsG0180004276.01.T01:CDS
GGTTTACAAATGAAACGGAA+AGG 0.664042 1:-75923126 MsG0180004276.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATTTACATTTTATTTT+AGG - Chr1:75923158-75923177 MsG0180004276.01.T01:CDS 10.0%
!! AAAATAAATAAGTAAACAAC+AGG + Chr1:75923460-75923479 None:intergenic 15.0%
!! AAAGTAAATCAAGTTAAAAA+AGG + Chr1:75922743-75922762 None:intergenic 15.0%
!! AATCAAGTTAAAAAAGGATT+AGG + Chr1:75922737-75922756 None:intergenic 20.0%
!!! AGTTATTTGTACACAATTTT+GGG - Chr1:75922848-75922867 MsG0180004276.01.T01:three_prime_UTR 20.0%
!!! TAGTTATTTGTACACAATTT+TGG - Chr1:75922847-75922866 MsG0180004276.01.T01:three_prime_UTR 20.0%
!! GTTATTTGTACACAATTTTG+GGG - Chr1:75922849-75922868 MsG0180004276.01.T01:three_prime_UTR 25.0%
AAAAAGTTGACGATTTGCAA+TGG + Chr1:75923284-75923303 None:intergenic 30.0%
AATTGCAATGTATACCAGTT+TGG - Chr1:75922678-75922697 MsG0180004276.01.T01:three_prime_UTR 30.0%
AGATTCACATTCAAAAGTGA+AGG + Chr1:75923102-75923121 None:intergenic 30.0%
ATTGCAATGTATACCAGTTT+GGG - Chr1:75922679-75922698 MsG0180004276.01.T01:three_prime_UTR 30.0%
GCAATTCACAGTAACAAATA+GGG + Chr1:75922664-75922683 None:intergenic 30.0%
GGAAATTAGTGATAATTGTG+GGG - Chr1:75922870-75922889 MsG0180004276.01.T01:three_prime_UTR 30.0%
GGGAAATTAGTGATAATTGT+GGG - Chr1:75922869-75922888 MsG0180004276.01.T01:three_prime_UTR 30.0%
TGCAATTCACAGTAACAAAT+AGG + Chr1:75922665-75922684 None:intergenic 30.0%
ACCATCAACTTCTTTGTCAT+TGG + Chr1:75923068-75923087 None:intergenic 35.0%
AGGCAGGTTTACAAATGAAA+CGG - Chr1:75922987-75923006 MsG0180004276.01.T01:intron 35.0%
ATCTCGTTTAGCATTGTTTC+TGG - Chr1:75923118-75923137 MsG0180004276.01.T01:CDS 35.0%
CAATACCTAAAGAACATGAG+GGG + Chr1:75922917-75922936 None:intergenic 35.0%
CCAATACCTAAAGAACATGA+GGG + Chr1:75922918-75922937 None:intergenic 35.0%
GGGGAAATTAGTGATAATTG+TGG - Chr1:75922868-75922887 MsG0180004276.01.T01:three_prime_UTR 35.0%
GGTTTACAAATGAAACGGAA+AGG - Chr1:75922992-75923011 MsG0180004276.01.T01:intron 35.0%
TCAACTTCTTTGTCATTGGA+TGG + Chr1:75923064-75923083 None:intergenic 35.0%
TCCAATGACAAAGAAGTTGA+TGG - Chr1:75923064-75923083 MsG0180004276.01.T01:CDS 35.0%
TTACAAATGAAACGGAAAGG+TGG - Chr1:75922995-75923014 MsG0180004276.01.T01:intron 35.0%
TTGAATAAAAGACTCTCCAC+AGG + Chr1:75922968-75922987 None:intergenic 35.0%
TTGTTAACTGCTTGATCATG+AGG - Chr1:75923416-75923435 MsG0180004276.01.T01:intron 35.0%
! TCTCGTTTAGCATTGTTTCT+GGG - Chr1:75923119-75923138 MsG0180004276.01.T01:CDS 35.0%
! TGTGGAGAGTCTTTTATTCA+AGG - Chr1:75922967-75922986 MsG0180004276.01.T01:intron 35.0%
!! TTTAGGTATTGGCTTTCTGT+TGG - Chr1:75922926-75922945 MsG0180004276.01.T01:CDS 35.0%
AAGTTGACGATTTGCAATGG+AGG + Chr1:75923281-75923300 None:intergenic 40.0%
ATGCATACATGACATGGTAG+AGG + Chr1:75923356-75923375 None:intergenic 40.0%
CCCTCATGTTCTTTAGGTAT+TGG - Chr1:75922915-75922934 MsG0180004276.01.T01:exon 40.0%
CGGCTTACAAGTTTAACTGA+GGG - Chr1:75923208-75923227 MsG0180004276.01.T01:intron 40.0%
GCAGAAGCTGTACTTTCTAT+CGG - Chr1:75923188-75923207 MsG0180004276.01.T01:CDS 40.0%
GCCAATACCTAAAGAACATG+AGG + Chr1:75922919-75922938 None:intergenic 40.0%
GTGATAATTGTGGGGATCAT+TGG - Chr1:75922878-75922897 MsG0180004276.01.T01:three_prime_UTR 40.0%
TCAAGCATGCATACATGACA+TGG + Chr1:75923362-75923381 None:intergenic 40.0%
TCGGCTTACAAGTTTAACTG+AGG - Chr1:75923207-75923226 MsG0180004276.01.T01:intron 40.0%
! AACAGCCTTATGTTTGGAGA+AGG - Chr1:75923234-75923253 MsG0180004276.01.T01:intron 40.0%
! GAGAGTCTTTTATTCAAGGC+AGG - Chr1:75922971-75922990 MsG0180004276.01.T01:intron 40.0%
! GGGAATAACAGCCTTATGTT+TGG - Chr1:75923228-75923247 MsG0180004276.01.T01:intron 40.0%
! TATACCAGTTTGGGATGAGA+AGG - Chr1:75922688-75922707 MsG0180004276.01.T01:three_prime_UTR 40.0%
AACATGAGGGGAGGTTAAGA+TGG + Chr1:75922905-75922924 None:intergenic 45.0%
AACCTCCCCTCATGTTCTTT+AGG - Chr1:75922909-75922928 MsG0180004276.01.T01:exon 45.0%
ACCCACCTTCTCCAAACATA+AGG + Chr1:75923242-75923261 None:intergenic 45.0%
GCCTTATGTTTGGAGAAGGT+GGG - Chr1:75923238-75923257 MsG0180004276.01.T01:intron 45.0%
TACCTAAAGAACATGAGGGG+AGG + Chr1:75922914-75922933 None:intergenic 45.0%
! AGCCTTATGTTTGGAGAAGG+TGG - Chr1:75923237-75923256 MsG0180004276.01.T01:intron 45.0%
TGCTCAACCTTCAGAACCTG+TGG - Chr1:75922949-75922968 MsG0180004276.01.T01:intron 50.0%
TTGGATGGCTGCTCTTCGTT+CGG + Chr1:75923049-75923068 None:intergenic 50.0%
! ACCAGTTTGGGATGAGAAGG+AGG - Chr1:75922691-75922710 MsG0180004276.01.T01:three_prime_UTR 50.0%
! AGACTCTCCACAGGTTCTGA+AGG + Chr1:75922959-75922978 None:intergenic 50.0%
! CCAGTTTGGGATGAGAAGGA+GGG - Chr1:75922692-75922711 MsG0180004276.01.T01:three_prime_UTR 50.0%
! CTTCGTTCGGGGTATCAACA+GGG + Chr1:75923036-75923055 None:intergenic 50.0%
! TCTTCGTTCGGGGTATCAAC+AGG + Chr1:75923037-75923056 None:intergenic 50.0%
!! AAACGGAAAGGTGGACTGTC+TGG - Chr1:75923004-75923023 MsG0180004276.01.T01:intron 50.0%
CCCTCCTTCTCATCCCAAAC+TGG + Chr1:75922695-75922714 None:intergenic 55.0%
TGGATGGCTGCTCTTCGTTC+GGG + Chr1:75923048-75923067 None:intergenic 55.0%
GGATGGCTGCTCTTCGTTCG+GGG + Chr1:75923047-75923066 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 75922641 75923499 75922641 ID=MsG0180004276.01;Name=MsG0180004276.01
Chr1 mRNA 75922641 75923499 75922641 ID=MsG0180004276.01.T01;Parent=MsG0180004276.01;Name=MsG0180004276.01.T01;_AED=0.48;_eAED=0.55;_QI=0|0.66|0.75|1|0.33|0.5|4|276|105
Chr1 exon 75922641 75922960 75922641 ID=MsG0180004276.01.T01:exon:14723;Parent=MsG0180004276.01.T01
Chr1 exon 75923055 75923209 75923055 ID=MsG0180004276.01.T01:exon:14722;Parent=MsG0180004276.01.T01
Chr1 exon 75923325 75923370 75923325 ID=MsG0180004276.01.T01:exon:14721;Parent=MsG0180004276.01.T01
Chr1 exon 75923427 75923499 75923427 ID=MsG0180004276.01.T01:exon:14720;Parent=MsG0180004276.01.T01
Chr1 CDS 75923427 75923499 75923427 ID=MsG0180004276.01.T01:cds;Parent=MsG0180004276.01.T01
Chr1 CDS 75923325 75923370 75923325 ID=MsG0180004276.01.T01:cds;Parent=MsG0180004276.01.T01
Chr1 CDS 75923055 75923209 75923055 ID=MsG0180004276.01.T01:cds;Parent=MsG0180004276.01.T01
Chr1 CDS 75922917 75922960 75922917 ID=MsG0180004276.01.T01:cds;Parent=MsG0180004276.01.T01
Chr1 three_prime_UTR 75922641 75922916 75922641 ID=MsG0180004276.01.T01:three_prime_utr;Parent=MsG0180004276.01.T01
Gene Sequence

>MsG0180004276.01.T01

ATGCAAGTTGCAAATGTCATCCCTATTTGTTACTGTGAATTGCAATGTATACCAGTTTGGGATGAGAAGGAGGCTAAAGATGATGAGAAAAGAGTGAACATCAAACTTGAAAGAGTGAAGTATTGGCTTTCTGTTGGTGCTCAACCTTCAGAACCTGTGGAGAGTCTTTTATTCAAGGCAGGTTTACAAATGAAACGGAAAGGTGGACTGTCTGGTAGCTACCCTGTTGATACCCCGAACGAAGAGCAGCCATCCAATGACAAAGAAGTTGATGGCGTATCTGCAGAAGCTGTACTTTCTATCGGCTTACAAGTTTAA

Protein sequence

>MsG0180004276.01.T01

MQVANVIPICYCELQCIPVWDEKEAKDDEKRVNIKLERVKYWLSVGAQPSEPVESLLFKAGLQMKRKGGLSGSYPVDTPNEEQPSNDKEVDGVSAEAVLSIGLQV*