AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020781.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020781.01.T01 MTR_4g058720 99.612 258 1 0 1 258 1 258 0.0 531
MsG0480020781.01.T01 MTR_7g116920 32.432 222 145 3 33 249 10 231 2.95e-30 113
MsG0480020781.01.T01 MTR_7g116920 32.683 205 133 3 50 249 9 213 5.60e-28 107
MsG0480020781.01.T01 MTR_7g076910 33.333 162 100 3 33 192 5 160 5.63e-18 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020781.01.T01 AT4G27490 73.152 257 67 2 1 256 1 256 6.44e-143 401
MsG0480020781.01.T01 AT4G27490 73.152 257 67 2 1 256 1 256 6.44e-143 401
MsG0480020781.01.T01 AT4G27490 73.152 257 67 2 1 256 1 256 6.44e-143 401
MsG0480020781.01.T01 AT4G27490 77.358 212 47 1 45 256 4 214 1.09e-123 351
MsG0480020781.01.T01 AT4G27490 71.123 187 53 1 1 186 1 187 1.06e-95 279
MsG0480020781.01.T01 AT4G27490 71.123 187 53 1 1 186 1 187 1.06e-95 279
MsG0480020781.01.T01 AT4G27490 71.123 187 53 1 1 186 1 187 1.06e-95 279
MsG0480020781.01.T01 AT3G61620 31.532 222 147 3 33 249 10 231 1.00e-28 109
MsG0480020781.01.T01 AT3G61620 31.532 222 147 3 33 249 10 231 1.00e-28 109
MsG0480020781.01.T01 AT3G46210 34.356 163 97 5 33 192 5 160 8.52e-17 77.8
MsG0480020781.01.T01 AT3G46210 34.356 163 97 5 33 192 5 160 8.52e-17 77.8
MsG0480020781.01.T01 AT3G46210 34.356 163 97 5 33 192 5 160 8.52e-17 77.8
MsG0480020781.01.T01 AT3G46210 34.356 163 97 5 33 192 5 160 8.52e-17 77.8
MsG0480020781.01.T01 AT3G46210 34.356 163 97 5 33 192 5 160 8.52e-17 77.8
MsG0480020781.01.T01 AT3G46210 34.356 163 97 5 33 192 5 160 8.52e-17 77.8
MsG0480020781.01.T01 AT3G46210 34.356 163 97 5 33 192 5 160 8.52e-17 77.8

Find 65 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGACCTCATTAATCTTT+GGG 0.282666 4:-48205578 None:intergenic
AGGCGGACCTCATTAATCTT+TGG 0.300741 4:-48205579 None:intergenic
TTTGGAGGGTGCAATAATAT+TGG 0.333785 4:+48203520 MsG0480020781.01.T01:CDS
GGATCTGCTTATGCTGAGTT+TGG 0.336443 4:+48203127 MsG0480020781.01.T01:CDS
ATTGCCTTCTTGCTCTCTCT+TGG 0.344667 4:-48203288 None:intergenic
TTCAACTCCGGTTCGTGGAC+AGG 0.376488 4:+48203362 MsG0480020781.01.T01:CDS
TTCGATGCTTCATAAAGCTT+TGG 0.379648 4:+48203502 MsG0480020781.01.T01:CDS
GAAACCACGGCCATCGGGTC+GGG 0.381446 4:-48202208 None:intergenic
AAAACATCCACAGTGGTCTT+GGG 0.383967 4:-48203551 None:intergenic
ATCACCCAACTGACAGTTAC+CGG 0.402501 4:+48205543 MsG0480020781.01.T01:CDS
GCAATGATGTACAGTGATAC+AGG 0.416564 4:+48203306 MsG0480020781.01.T01:CDS
CGTCATTGATCCTATATTTG+AGG 0.429457 4:+48205464 MsG0480020781.01.T01:CDS
GGTGCTGTGAATGCTGCATC+AGG 0.440274 4:+48203106 MsG0480020781.01.T01:CDS
GCAGGGGATGCAGCTGTGCT+TGG 0.440538 4:+48205958 MsG0480020781.01.T01:intron
CTAGCAACCGATATGACAAC+TGG 0.441517 4:-48204869 None:intergenic
GCATCGAACTGTACTCTTTG+TGG 0.445785 4:-48203487 None:intergenic
TGCTTCTCAAGGAAGAAGAT+TGG 0.451632 4:+48202184 MsG0480020781.01.T01:CDS
TACTCACCGTCTCTGACAAC+CGG 0.464034 4:+48202147 MsG0480020781.01.T01:CDS
ATCGGTTGCTAGTCTTGCCT+TGG 0.469618 4:+48204880 MsG0480020781.01.T01:CDS
GATGCTTCATAAAGCTTTGG+AGG 0.481702 4:+48203505 MsG0480020781.01.T01:CDS
CAAAGCTTGCTAAGATTATG+AGG 0.489962 4:+48205990 MsG0480020781.01.T01:CDS
TGGACCCGACCCGATGGCCG+TGG 0.491907 4:+48202204 MsG0480020781.01.T01:CDS
TTGGGAATGGCCGGCAAAAG+TGG 0.492601 4:+48202108 None:intergenic
TAATATACCTGTCCACGAAC+CGG 0.492612 4:-48203369 None:intergenic
TAATCATTAAGCTTCCATCT+TGG 0.496219 4:-48205497 None:intergenic
CTGGTGGAAACCACGGCCAT+CGG 0.497666 4:-48202214 None:intergenic
GGAAACCACGGCCATCGGGT+CGG 0.497786 4:-48202209 None:intergenic
TGGTGGAAACCACGGCCATC+GGG 0.498963 4:-48202213 None:intergenic
TGATCTCCCAGTTGTCATAT+CGG 0.507012 4:+48204862 MsG0480020781.01.T01:CDS
TTCGCCGGTAACTGTCAGTT+GGG 0.515084 4:-48205547 None:intergenic
GACCTCATTAATCTTTGGGG+TGG 0.519184 4:-48205574 None:intergenic
AAGGTCATACATCAGTATTC+CGG 0.520451 4:-48204909 None:intergenic
TCACACAGATACAATGACTT+TGG 0.523678 4:-48203155 None:intergenic
AGTCTTGCCTTGGCAGATGC+CGG 0.528894 4:+48204890 MsG0480020781.01.T01:CDS
CTTTGGGGTGGACCATTCGC+CGG 0.530416 4:-48205562 None:intergenic
TAATGATTCCAGTCATGTCT+CGG 0.532516 4:+48205432 MsG0480020781.01.T01:intron
GAGGAGGAAAACTGCCAAGA+TGG 0.537446 4:+48205483 MsG0480020781.01.T01:CDS
AGAATCTGCTTCTGATGCAC+AGG 0.537718 4:+48206024 MsG0480020781.01.T01:CDS
ACACCGCCGTTGGGAATGGC+CGG 0.541283 4:+48202099 None:intergenic
ATTCGCCGGTAACTGTCAGT+TGG 0.542035 4:-48205548 None:intergenic
TCTTCTTCCTTGAGAAGCAG+TGG 0.551293 4:-48202180 None:intergenic
CGGCAAAAGTGGAACGACTC+CGG 0.565948 4:+48202119 MsG0480020781.01.T01:CDS
AAAGAAACCACTGCTTCTCA+AGG 0.571189 4:+48202173 MsG0480020781.01.T01:CDS
GAAGATTGGACCCGACCCGA+TGG 0.573254 4:+48202198 MsG0480020781.01.T01:CDS
AAACATCCACAGTGGTCTTG+GGG 0.573612 4:-48203550 None:intergenic
GCTTACAAGCGGGTCTACAC+TGG 0.579283 4:-48202233 None:intergenic
ATGCTTCATAAAGCTTTGGA+GGG 0.579684 4:+48203506 MsG0480020781.01.T01:CDS
GTCTACACTGGTGGAAACCA+CGG 0.581502 4:-48202221 None:intergenic
CAGTATTCCGGCATCTGCCA+AGG 0.585424 4:-48204897 None:intergenic
TACAAGCGGGTCTACACTGG+TGG 0.590371 4:-48202230 None:intergenic
CCTTGTCGCCTCAGTTTCAG+TGG 0.591419 4:+48204928 MsG0480020781.01.T01:CDS
AATTCATACCACTGAAACTG+AGG 0.596309 4:-48204936 None:intergenic
CAATGATGTACAGTGATACA+GGG 0.609152 4:+48203307 MsG0480020781.01.T01:CDS
AACTGACAGTTACCGGCGAA+TGG 0.609296 4:+48205550 MsG0480020781.01.T01:CDS
CATTGATCCTATATTTGAGG+AGG 0.611322 4:+48205467 MsG0480020781.01.T01:CDS
CCACTGAAACTGAGGCGACA+AGG 0.615029 4:-48204928 None:intergenic
GCGGACCTCATTAATCTTTG+GGG 0.618854 4:-48205577 None:intergenic
GTCCACCCCAAAGATTAATG+AGG 0.632706 4:+48205572 MsG0480020781.01.T01:CDS
TGGGTGATTTCATAGCGAGA+AGG 0.637919 4:-48205528 None:intergenic
AGAGACGGTGAGTATGTCGC+CGG 0.644092 4:-48202138 None:intergenic
TGGGCCAAGAGAGAGCAAGA+AGG 0.650542 4:+48203284 MsG0480020781.01.T01:CDS
GCATTGGTGCTGGAATCTAG+TGG 0.655397 4:+48203575 MsG0480020781.01.T01:CDS
CAAATATAGGATCAATGACG+AGG 0.670852 4:-48205461 None:intergenic
TTCTTTCCCCAAGACCACTG+TGG 0.680542 4:+48203544 MsG0480020781.01.T01:CDS
TAGCAACCGATATGACAACT+GGG 0.691620 4:-48204868 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAATAAATTATT+AGG - Chr4:48202293-48202312 None:intergenic 0.0%
!! AAATAAATAATAAATTATTA+GGG - Chr4:48202292-48202311 None:intergenic 0.0%
!! ATAAAACATATATAGATTAA+TGG + Chr4:48205844-48205863 MsG0480020781.01.T01:intron 10.0%
!! GTCAAAAAAAAAAAAAATTA+AGG - Chr4:48205320-48205339 None:intergenic 10.0%
!! TAAAACATATATAGATTAAT+GGG + Chr4:48205845-48205864 MsG0480020781.01.T01:intron 10.0%
!! AAAATAAACAAGATATGATA+AGG - Chr4:48203423-48203442 None:intergenic 15.0%
!! ATCATAAAGAAATATTTCTT+TGG + Chr4:48204681-48204700 MsG0480020781.01.T01:intron 15.0%
!! CTAAAATATAAGCAAAAAAT+GGG + Chr4:48202444-48202463 MsG0480020781.01.T01:intron 15.0%
!! TAATTAGCTAAATGAAAATA+TGG - Chr4:48203833-48203852 None:intergenic 15.0%
!! TACCTTAATTTATATAGTAA+AGG - Chr4:48204607-48204626 None:intergenic 15.0%
!! TTTACTATATAAATTAAGGT+AGG + Chr4:48204606-48204625 MsG0480020781.01.T01:intron 15.0%
!!! AATCTATATATGTTTTATTG+CGG - Chr4:48205842-48205861 None:intergenic 15.0%
!!! ATTATTTTCTCCTAAATTTT+AGG - Chr4:48204428-48204447 None:intergenic 15.0%
!!! CATTTTTTGCTTATATTTTA+GGG - Chr4:48202445-48202464 None:intergenic 15.0%
!!! TTATTTTCTCCTAAATTTTA+GGG - Chr4:48204427-48204446 None:intergenic 15.0%
!! AAAAATTAAGGAATAGTTCA+TGG - Chr4:48205308-48205327 None:intergenic 20.0%
!! CAATTGGATTAATCAAATTA+AGG - Chr4:48205887-48205906 None:intergenic 20.0%
!! CACCTTTACTATATAAATTA+AGG + Chr4:48204602-48204621 MsG0480020781.01.T01:intron 20.0%
!! CCTAAAATATAAGCAAAAAA+TGG + Chr4:48202443-48202462 MsG0480020781.01.T01:intron 20.0%
!! GTAAATTAAAAACGTGAAAT+AGG - Chr4:48205644-48205663 None:intergenic 20.0%
!! TCTTTAATTATTAAGCATGA+CGG - Chr4:48202641-48202660 None:intergenic 20.0%
!!! AAGGATCGTATTTAATATTT+TGG + Chr4:48204739-48204758 MsG0480020781.01.T01:intron 20.0%
!!! AATTTTTTAGATTGTTTGAC+TGG + Chr4:48204349-48204368 MsG0480020781.01.T01:intron 20.0%
!!! ACTGTTTTTTATTTATTCTG+TGG + Chr4:48205170-48205189 MsG0480020781.01.T01:intron 20.0%
!!! ATAATTTTGCAGATTTTTCT+GGG + Chr4:48204579-48204598 MsG0480020781.01.T01:intron 20.0%
!!! CCATTTTTTGCTTATATTTT+AGG - Chr4:48202446-48202465 None:intergenic 20.0%
!!! TAGCATGGTTTTTTAAATTA+TGG + Chr4:48205779-48205798 MsG0480020781.01.T01:intron 20.0%
!!! TTTCATTAGTTTTTGTTTGT+TGG + Chr4:48203237-48203256 MsG0480020781.01.T01:intron 20.0%
! AATTGCACAACAAATATAGA+TGG + Chr4:48205902-48205921 MsG0480020781.01.T01:intron 25.0%
! AGACTAAAAATAAGTGTACA+AGG + Chr4:48202588-48202607 MsG0480020781.01.T01:intron 25.0%
! ATTTAAAAAAGATGGAGAAC+AGG - Chr4:48205602-48205621 None:intergenic 25.0%
! ATTTAAATCAATTGCAACTC+TGG - Chr4:48204768-48204787 None:intergenic 25.0%
! CATTTAGCTAATTAATCATG+AGG + Chr4:48203839-48203858 MsG0480020781.01.T01:intron 25.0%
! CGACAATAGTTTGATAAAAA+AGG + Chr4:48202671-48202690 MsG0480020781.01.T01:intron 25.0%
! GAAAGATCAACCAATAAAAA+TGG - Chr4:48205233-48205252 None:intergenic 25.0%
! GTGATTCTCTCTAAATATTA+AGG - Chr4:48205817-48205836 None:intergenic 25.0%
! GTTTGATAAAAAAGGATTTG+TGG + Chr4:48202679-48202698 MsG0480020781.01.T01:intron 25.0%
! TTAACTATAACTACATGTGT+CGG - Chr4:48202937-48202956 None:intergenic 25.0%
! TTAGTGTATTCTAAGTTGTA+AGG + Chr4:48204114-48204133 MsG0480020781.01.T01:intron 25.0%
! TTTATTATTGAATTTGTCGC+AGG + Chr4:48204839-48204858 MsG0480020781.01.T01:intron 25.0%
! TTTCGTTATCCCTAAAATTT+AGG + Chr4:48204415-48204434 MsG0480020781.01.T01:intron 25.0%
! TTTCTGGATTTATTAATGCT+AGG + Chr4:48205249-48205268 MsG0480020781.01.T01:intron 25.0%
!! AAACACCATCATTTTATTCT+AGG - Chr4:48203907-48203926 None:intergenic 25.0%
!! ATGTGTTCTTTTAATAGGTT+TGG + Chr4:48203264-48203283 MsG0480020781.01.T01:intron 25.0%
!! ATTTTGCTTACATTATGAGA+CGG + Chr4:48202526-48202545 MsG0480020781.01.T01:intron 25.0%
!! CATAATTTTGCAGATTTTTC+TGG + Chr4:48204578-48204597 MsG0480020781.01.T01:intron 25.0%
!! TGGATAGGTTAGAAATTTTA+AGG + Chr4:48203657-48203676 MsG0480020781.01.T01:intron 25.0%
!! TGTGTTCTTTTAATAGGTTT+GGG + Chr4:48203265-48203284 MsG0480020781.01.T01:intron 25.0%
!! TTTAGTTGAAGTACTATCAT+AGG + Chr4:48204013-48204032 MsG0480020781.01.T01:intron 25.0%
!!! ATCTATATTTGTTGTGCAAT+TGG - Chr4:48205903-48205922 None:intergenic 25.0%
!!! CATAGTCATATTGTTTTTGA+TGG + Chr4:48203637-48203656 MsG0480020781.01.T01:intron 25.0%
!!! GATCGTATTTAATATTTTGG+AGG + Chr4:48204742-48204761 MsG0480020781.01.T01:intron 25.0%
!!! TAATAGTTGTTTTACACAAC+TGG + Chr4:48204536-48204555 MsG0480020781.01.T01:intron 25.0%
!!! TATGTTTTAATGCTTGAAAC+AGG + Chr4:48205750-48205769 MsG0480020781.01.T01:intron 25.0%
!!! TCACGTTTTTAATTTACTTC+AGG + Chr4:48205647-48205666 MsG0480020781.01.T01:intron 25.0%
!!! TCATATTGTTTTTGATGGAT+AGG + Chr4:48203642-48203661 MsG0480020781.01.T01:intron 25.0%
!!! TTGTTTACTGTGTTTTTATC+TGG + Chr4:48203747-48203766 MsG0480020781.01.T01:intron 25.0%
!!! TTTGCTTATATTTTAGGGTT+TGG - Chr4:48202440-48202459 None:intergenic 25.0%
!!! TTTTTATTGGTTGATCTTTC+TGG + Chr4:48205233-48205252 MsG0480020781.01.T01:intron 25.0%
AAGATATAGCCAAGTACATA+CGG + Chr4:48204497-48204516 MsG0480020781.01.T01:intron 30.0%
AAGCTTATTTCAAGACTTGT+TGG + Chr4:48203950-48203969 MsG0480020781.01.T01:intron 30.0%
ACAGTTTAACAAGCATTTCA+AGG - Chr4:48205155-48205174 None:intergenic 30.0%
ACCTATTAAAAGAACACATC+AGG - Chr4:48203263-48203282 None:intergenic 30.0%
ACTCACCTAGAATAAAATGA+TGG + Chr4:48203899-48203918 MsG0480020781.01.T01:intron 30.0%
ATAGACTTCTTGCAACAAAA+AGG - Chr4:48204293-48204312 None:intergenic 30.0%
ATCTTATATCTCGCAAAGTT+AGG + Chr4:48203457-48203476 MsG0480020781.01.T01:intron 30.0%
CAAACTATTGTCGTACTTTA+GGG - Chr4:48202664-48202683 None:intergenic 30.0%
CTACAAACACGAAATCATAA+AGG - Chr4:48203075-48203094 None:intergenic 30.0%
TAATATATCCCGTATGTACT+TGG - Chr4:48204509-48204528 None:intergenic 30.0%
TAATCATTAAGCTTCCATCT+TGG - Chr4:48205500-48205519 None:intergenic 30.0%
TCAAACTATTGTCGTACTTT+AGG - Chr4:48202665-48202684 None:intergenic 30.0%
TCCTTACAAAACAGAAATCT+TGG - Chr4:48204058-48204077 None:intergenic 30.0%
TCTGGTGCAAAGTAAAATAT+TGG - Chr4:48205075-48205094 None:intergenic 30.0%
TCTTATATCTCGCAAAGTTA+GGG + Chr4:48203458-48203477 MsG0480020781.01.T01:intron 30.0%
TCTTGAAATAAGCTTCACTA+AGG - Chr4:48203945-48203964 None:intergenic 30.0%
TGTACTTGGCTATATCTTTA+AGG - Chr4:48204495-48204514 None:intergenic 30.0%
TTAATGTGTACAAGAGTTCA+TGG + Chr4:48202326-48202345 MsG0480020781.01.T01:intron 30.0%
TTGTCAAATTTAAGAAGCAG+TGG + Chr4:48204714-48204733 MsG0480020781.01.T01:intron 30.0%
! ACCAAGATTTCTGTTTTGTA+AGG + Chr4:48204054-48204073 MsG0480020781.01.T01:intron 30.0%
! ATTTCGTGTTTGTAGTTTTC+AGG + Chr4:48203080-48203099 MsG0480020781.01.T01:intron 30.0%
! CTTTTCCTATAATAGACTTC+CGG - Chr4:48204147-48204166 None:intergenic 30.0%
!! AATTTGTGCCTTGGTTTTTA+GGG + Chr4:48204982-48205001 MsG0480020781.01.T01:intron 30.0%
!! TAATTTGTGCCTTGGTTTTT+AGG + Chr4:48204981-48205000 MsG0480020781.01.T01:intron 30.0%
!!! TTTGGCATTGCCATTTTTAT+TGG + Chr4:48205220-48205239 MsG0480020781.01.T01:intron 30.0%
AAGGTCATACATCAGTATTC+CGG - Chr4:48204912-48204931 None:intergenic 35.0%
AATTACTCCACGCTCTATAA+GGG - Chr4:48204250-48204269 None:intergenic 35.0%
AATTCATACCACTGAAACTG+AGG - Chr4:48204939-48204958 None:intergenic 35.0%
AATTTAAGAAGCAGTGGTGA+AGG + Chr4:48204720-48204739 MsG0480020781.01.T01:intron 35.0%
ACACGGCTTTCAATATGAAT+TGG - Chr4:48202901-48202920 None:intergenic 35.0%
ACTGCCACATATTCTATACT+AGG - Chr4:48204195-48204214 None:intergenic 35.0%
AGATATAGCCAAGTACATAC+GGG + Chr4:48204498-48204517 MsG0480020781.01.T01:intron 35.0%
AGCAAACACAATAACGGTTT+GGG + Chr4:48202724-48202743 MsG0480020781.01.T01:intron 35.0%
ATGCCTTCAATTAGAAGAGT+TGG + Chr4:48202828-48202847 MsG0480020781.01.T01:intron 35.0%
ATTAAGGAATAGTTCATGGC+AGG - Chr4:48205304-48205323 None:intergenic 35.0%
CAAATATAGGATCAATGACG+AGG - Chr4:48205464-48205483 None:intergenic 35.0%
CAATGATGTACAGTGATACA+GGG + Chr4:48203307-48203326 MsG0480020781.01.T01:CDS 35.0%
CACGGCTTTCAATATGAATT+GGG - Chr4:48202900-48202919 None:intergenic 35.0%
CATTGATCCTATATTTGAGG+AGG + Chr4:48205467-48205486 MsG0480020781.01.T01:CDS 35.0%
CCTCATAGCAAACACAATAA+CGG + Chr4:48202718-48202737 MsG0480020781.01.T01:intron 35.0%
CGGAAGTCTATTATAGGAAA+AGG + Chr4:48204145-48204164 MsG0480020781.01.T01:intron 35.0%
CGTCATTGATCCTATATTTG+AGG + Chr4:48205464-48205483 MsG0480020781.01.T01:CDS 35.0%
CTGCCACATATTCTATACTA+GGG - Chr4:48204194-48204213 None:intergenic 35.0%
GATAGAAGTCTAGTTGTTAG+TGG + Chr4:48204643-48204662 MsG0480020781.01.T01:intron 35.0%
GATCCCTAGTATAGAATATG+TGG + Chr4:48204188-48204207 MsG0480020781.01.T01:intron 35.0%
GTATTCTAAGTTGTAAGGTG+AGG + Chr4:48204119-48204138 MsG0480020781.01.T01:intron 35.0%
GTTCAAAGAATCCTAACACT+AGG + Chr4:48204466-48204485 MsG0480020781.01.T01:intron 35.0%
TAAAAAAGATGGAGAACAGG+CGG - Chr4:48205599-48205618 None:intergenic 35.0%
TAATGATTCCAGTCATGTCT+CGG + Chr4:48205432-48205451 MsG0480020781.01.T01:intron 35.0%
TAATTACTCCACGCTCTATA+AGG - Chr4:48204251-48204270 None:intergenic 35.0%
TACTGTCTCACTTTACAGAT+TGG + Chr4:48205671-48205690 MsG0480020781.01.T01:intron 35.0%
TAGCAAACACAATAACGGTT+TGG + Chr4:48202723-48202742 MsG0480020781.01.T01:intron 35.0%
TAGGATTCTTTGAACATCAG+TGG - Chr4:48204461-48204480 None:intergenic 35.0%
TCACACAGATACAATGACTT+TGG - Chr4:48203158-48203177 None:intergenic 35.0%
TTAAGGAATAGTTCATGGCA+GGG - Chr4:48205303-48205322 None:intergenic 35.0%
TTCGATGCTTCATAAAGCTT+TGG + Chr4:48203502-48203521 MsG0480020781.01.T01:CDS 35.0%
TTGAACATCAGTGGTTGAAA+GGG - Chr4:48204452-48204471 None:intergenic 35.0%
TTTGAACATCAGTGGTTGAA+AGG - Chr4:48204453-48204472 None:intergenic 35.0%
TTTGCAACCTAAGCTACATA+CGG + Chr4:48203872-48203891 MsG0480020781.01.T01:intron 35.0%
! AAGCAGTGGTTTCTTTTTAC+CGG - Chr4:48202169-48202188 None:intergenic 35.0%
! ATTCTCTGTAATTTGTGCCT+TGG + Chr4:48204973-48204992 MsG0480020781.01.T01:intron 35.0%
! CAGTTTTCCTCCTCAAATAT+AGG - Chr4:48205477-48205496 None:intergenic 35.0%
! CATGCTGATTGAAGTATGAA+GGG - Chr4:48203208-48203227 None:intergenic 35.0%
! CTATACAACGTTTTCAACTC+CGG + Chr4:48203350-48203369 MsG0480020781.01.T01:CDS 35.0%
! CTTTAAGGAATCCTAGTGTT+AGG - Chr4:48204480-48204499 None:intergenic 35.0%
! GCCTGATGTGTTCTTTTAAT+AGG + Chr4:48203259-48203278 MsG0480020781.01.T01:intron 35.0%
! TCATGCTGATTGAAGTATGA+AGG - Chr4:48203209-48203228 None:intergenic 35.0%
! TGATGGTGTTTGAATCTAAC+TGG + Chr4:48203916-48203935 MsG0480020781.01.T01:intron 35.0%
! TTTGGAGGGTGCAATAATAT+TGG + Chr4:48203520-48203539 MsG0480020781.01.T01:CDS 35.0%
!! AAAAAGCACCCTAAAAACCA+AGG - Chr4:48204993-48205012 None:intergenic 35.0%
!! ATGCTTCATAAAGCTTTGGA+GGG + Chr4:48203506-48203525 MsG0480020781.01.T01:CDS 35.0%
!! ATTTCAAGACTTGTTGGTCT+GGG + Chr4:48203956-48203975 MsG0480020781.01.T01:intron 35.0%
!! CAAAGCTTGCTAAGATTATG+AGG + Chr4:48205990-48206009 MsG0480020781.01.T01:CDS 35.0%
!! CGTTATTGTGTTTGCTATGA+GGG - Chr4:48202720-48202739 None:intergenic 35.0%
!! TATTTCAAGACTTGTTGGTC+TGG + Chr4:48203955-48203974 MsG0480020781.01.T01:intron 35.0%
AAAAACATCCACAGTGGTCT+TGG - Chr4:48203555-48203574 None:intergenic 40.0%
AAAACATCCACAGTGGTCTT+GGG - Chr4:48203554-48203573 None:intergenic 40.0%
AAAGAAACCACTGCTTCTCA+AGG + Chr4:48202173-48202192 MsG0480020781.01.T01:CDS 40.0%
AACAAGCATTTCAAGGCTAC+AGG - Chr4:48205148-48205167 None:intergenic 40.0%
AGAGTGATGAAGCTTACAAG+CGG - Chr4:48202247-48202266 None:intergenic 40.0%
AGCAAGCTCTCATTGTCATT+TGG + Chr4:48205925-48205944 MsG0480020781.01.T01:intron 40.0%
ATGCATAGAGTGTGTGATTC+TGG - Chr4:48205093-48205112 None:intergenic 40.0%
ATGCTATCGTGCATAGAGTA+TGG - Chr4:48205199-48205218 None:intergenic 40.0%
ATTACTCCACGCTCTATAAG+GGG - Chr4:48204249-48204268 None:intergenic 40.0%
ATTATTCCCCTTATAGAGCG+TGG + Chr4:48204240-48204259 MsG0480020781.01.T01:intron 40.0%
CAATGCAAAAACATCCACAG+TGG - Chr4:48203561-48203580 None:intergenic 40.0%
GCAAACACAATAACGGTTTG+GGG + Chr4:48202725-48202744 MsG0480020781.01.T01:intron 40.0%
GCAATGATGTACAGTGATAC+AGG + Chr4:48203306-48203325 MsG0480020781.01.T01:CDS 40.0%
GCACCAACTCTTCTAATTGA+AGG - Chr4:48202834-48202853 None:intergenic 40.0%
TAAGACATGCTGTGTTGTCA+TGG + Chr4:48202402-48202421 MsG0480020781.01.T01:intron 40.0%
TAATATACCTGTCCACGAAC+CGG - Chr4:48203372-48203391 None:intergenic 40.0%
TAGCAACCGATATGACAACT+GGG - Chr4:48204871-48204890 None:intergenic 40.0%
TCGGTATCAGATATTGGACA+CGG - Chr4:48202918-48202937 None:intergenic 40.0%
TCTATGCACGATAGCATGTT+TGG + Chr4:48205202-48205221 MsG0480020781.01.T01:intron 40.0%
TGAACATCAGTGGTTGAAAG+GGG - Chr4:48204451-48204470 None:intergenic 40.0%
TGATCTCCCAGTTGTCATAT+CGG + Chr4:48204862-48204881 MsG0480020781.01.T01:CDS 40.0%
TGCTTACATTATGAGACGGA+GGG + Chr4:48202530-48202549 MsG0480020781.01.T01:intron 40.0%
TGCTTCTCAAGGAAGAAGAT+TGG + Chr4:48202184-48202203 MsG0480020781.01.T01:CDS 40.0%
TTGCTTACATTATGAGACGG+AGG + Chr4:48202529-48202548 MsG0480020781.01.T01:intron 40.0%
! ATTTGGTTTCCTTCATGCAG+GGG + Chr4:48205942-48205961 MsG0480020781.01.T01:intron 40.0%
! CATGTGTCGGTATCAGATAT+TGG - Chr4:48202924-48202943 None:intergenic 40.0%
! CATTTGGTTTCCTTCATGCA+GGG + Chr4:48205941-48205960 MsG0480020781.01.T01:intron 40.0%
! GATGCTTCATAAAGCTTTGG+AGG + Chr4:48203505-48203524 MsG0480020781.01.T01:CDS 40.0%
! TCATTTGGTTTCCTTCATGC+AGG + Chr4:48205940-48205959 MsG0480020781.01.T01:intron 40.0%
! TGAAGTACTATCATAGGCAC+TGG + Chr4:48204019-48204038 MsG0480020781.01.T01:intron 40.0%
! TGGATTCAGCACAAGACTTT+TGG - Chr4:48202751-48202770 None:intergenic 40.0%
!! AGACTTCTAGCTTCTGTGTA+AGG + Chr4:48204317-48204336 MsG0480020781.01.T01:intron 40.0%
!! CACTGTGGATGTTTTTGCAT+TGG + Chr4:48203559-48203578 MsG0480020781.01.T01:CDS 40.0%
!! CCGTTATTGTGTTTGCTATG+AGG - Chr4:48202721-48202740 None:intergenic 40.0%
!! GTGTTTGTAGTTTTCAGGAC+TGG + Chr4:48203085-48203104 MsG0480020781.01.T01:intron 40.0%
!! TGAAACAGGAGTATCTAGCA+TGG + Chr4:48205764-48205783 MsG0480020781.01.T01:intron 40.0%
!! TTTTTACCGGTTGTCAGAGA+CGG - Chr4:48202156-48202175 None:intergenic 40.0%
!!! TGTTTGCTATGAGGGTTTTG+AGG - Chr4:48202712-48202731 None:intergenic 40.0%
ACCTCTTCGCATTAACGCAA+GGG - Chr4:48203799-48203818 None:intergenic 45.0%
AGAATCTGCTTCTGATGCAC+AGG + Chr4:48206024-48206043 MsG0480020781.01.T01:CDS 45.0%
AGGCGGACCTCATTAATCTT+TGG - Chr4:48205582-48205601 None:intergenic 45.0%
AGTGCATCCGTATGTAGCTT+AGG - Chr4:48203882-48203901 None:intergenic 45.0%
ATCACCCAACTGACAGTTAC+CGG + Chr4:48205543-48205562 MsG0480020781.01.T01:CDS 45.0%
ATGCCTTCAACATGAAGCGT+TGG - Chr4:48202876-48202895 None:intergenic 45.0%
ATTGCCTTCTTGCTCTCTCT+TGG - Chr4:48203291-48203310 None:intergenic 45.0%
CACATGATATGACGCTGACA+TGG - Chr4:48203000-48203019 None:intergenic 45.0%
CTAGCAACCGATATGACAAC+TGG - Chr4:48204872-48204891 None:intergenic 45.0%
GAGTGATGAAGCTTACAAGC+GGG - Chr4:48202246-48202265 None:intergenic 45.0%
GCATCGAACTGTACTCTTTG+TGG - Chr4:48203490-48203509 None:intergenic 45.0%
GCGGACCTCATTAATCTTTG+GGG - Chr4:48205580-48205599 None:intergenic 45.0%
GGATCTGCTTATGCTGAGTT+TGG + Chr4:48203127-48203146 MsG0480020781.01.T01:CDS 45.0%
GGCGGACCTCATTAATCTTT+GGG - Chr4:48205581-48205600 None:intergenic 45.0%
GTCCACCCCAAAGATTAATG+AGG + Chr4:48205572-48205591 MsG0480020781.01.T01:CDS 45.0%
TAAGTTGTAAGGTGAGGCAC+CGG + Chr4:48204125-48204144 MsG0480020781.01.T01:intron 45.0%
TACCTCTTCGCATTAACGCA+AGG - Chr4:48203800-48203819 None:intergenic 45.0%
TCTTCTTCCTTGAGAAGCAG+TGG - Chr4:48202183-48202202 None:intergenic 45.0%
TGGGTGATTTCATAGCGAGA+AGG - Chr4:48205531-48205550 None:intergenic 45.0%
! AAACATCCACAGTGGTCTTG+GGG - Chr4:48203553-48203572 None:intergenic 45.0%
! GACCTCATTAATCTTTGGGG+TGG - Chr4:48205577-48205596 None:intergenic 45.0%
! GGAGGGAGTATAGATGATAG+TGG + Chr4:48202547-48202566 MsG0480020781.01.T01:intron 45.0%
! TGCCTTCAACATGAAGCGTT+GGG - Chr4:48202875-48202894 None:intergenic 45.0%
!! AGGCACCGGAAGTCTATTAT+AGG + Chr4:48204139-48204158 MsG0480020781.01.T01:intron 45.0%
!! GGATGTTTTTGCATTGGTGC+TGG + Chr4:48203565-48203584 MsG0480020781.01.T01:CDS 45.0%
!! ATAAAAATATTTAAAAAAGA+TGG - Chr4:48205610-48205629 None:intergenic 5.0%
AACTGACAGTTACCGGCGAA+TGG + Chr4:48205550-48205569 MsG0480020781.01.T01:CDS 50.0%
ACCCCAACGCTTCATGTTGA+AGG + Chr4:48202870-48202889 MsG0480020781.01.T01:intron 50.0%
AGCAGAATAGCATTGCGCTG+CGG - Chr4:48202778-48202797 None:intergenic 50.0%
ATTCGCCGGTAACTGTCAGT+TGG - Chr4:48205551-48205570 None:intergenic 50.0%
GAGGAGGAAAACTGCCAAGA+TGG + Chr4:48205483-48205502 MsG0480020781.01.T01:CDS 50.0%
GGTTCTTACCGAGACATGAC+TGG - Chr4:48205443-48205462 None:intergenic 50.0%
GTCTACACTGGTGGAAACCA+CGG - Chr4:48202224-48202243 None:intergenic 50.0%
TACTCACCGTCTCTGACAAC+CGG + Chr4:48202147-48202166 MsG0480020781.01.T01:CDS 50.0%
TCCCTTGCGTTAATGCGAAG+AGG + Chr4:48203795-48203814 MsG0480020781.01.T01:intron 50.0%
TTCGCCGGTAACTGTCAGTT+GGG - Chr4:48205550-48205569 None:intergenic 50.0%
TTCTTTCCCCAAGACCACTG+TGG + Chr4:48203544-48203563 MsG0480020781.01.T01:CDS 50.0%
TTGGTCTGGGTACAGCTTGT+AGG + Chr4:48203969-48203988 MsG0480020781.01.T01:intron 50.0%
!! ACGTTTTCAACTCCGGTTCG+TGG + Chr4:48203357-48203376 MsG0480020781.01.T01:CDS 50.0%
!! ATCGGTTGCTAGTCTTGCCT+TGG + Chr4:48204880-48204899 MsG0480020781.01.T01:CDS 50.0%
!! GCATTGGTGCTGGAATCTAG+TGG + Chr4:48203575-48203594 MsG0480020781.01.T01:CDS 50.0%
!! GCCTTCAACATGAAGCGTTG+GGG - Chr4:48202874-48202893 None:intergenic 50.0%
AGAGACGGTGAGTATGTCGC+CGG - Chr4:48202141-48202160 None:intergenic 55.0%
AGTCTTGCCTTGGCAGATGC+CGG + Chr4:48204890-48204909 MsG0480020781.01.T01:CDS 55.0%
CAGTATTCCGGCATCTGCCA+AGG - Chr4:48204900-48204919 None:intergenic 55.0%
CCACTGAAACTGAGGCGACA+AGG - Chr4:48204931-48204950 None:intergenic 55.0%
CCTTGTCGCCTCAGTTTCAG+TGG + Chr4:48204928-48204947 MsG0480020781.01.T01:CDS 55.0%
CGGCAAAAGTGGAACGACTC+CGG + Chr4:48202119-48202138 MsG0480020781.01.T01:CDS 55.0%
GCTTACAAGCGGGTCTACAC+TGG - Chr4:48202236-48202255 None:intergenic 55.0%
TACAAGCGGGTCTACACTGG+TGG - Chr4:48202233-48202252 None:intergenic 55.0%
TGGGCCAAGAGAGAGCAAGA+AGG + Chr4:48203284-48203303 MsG0480020781.01.T01:CDS 55.0%
! GGTGCTGTGAATGCTGCATC+AGG + Chr4:48203106-48203125 MsG0480020781.01.T01:CDS 55.0%
! TTCAACTCCGGTTCGTGGAC+AGG + Chr4:48203362-48203381 MsG0480020781.01.T01:CDS 55.0%
!! CGGAGTCGTTCCACTTTTGC+CGG - Chr4:48202121-48202140 None:intergenic 55.0%
CAGCTGCATCCCCTGCATGA+AGG - Chr4:48205954-48205973 None:intergenic 60.0%
CTGGTGGAAACCACGGCCAT+CGG - Chr4:48202217-48202236 None:intergenic 60.0%
GAAGATTGGACCCGACCCGA+TGG + Chr4:48202198-48202217 MsG0480020781.01.T01:CDS 60.0%
TAGCATTGCGCTGCGGCGAT+TGG - Chr4:48202771-48202790 None:intergenic 60.0%
TGGTGGAAACCACGGCCATC+GGG - Chr4:48202216-48202235 None:intergenic 60.0%
!! CTTTGGGGTGGACCATTCGC+CGG - Chr4:48205565-48205584 None:intergenic 60.0%
GAAACCACGGCCATCGGGTC+GGG - Chr4:48202211-48202230 None:intergenic 65.0%
GCAGGGGATGCAGCTGTGCT+TGG + Chr4:48205958-48205977 MsG0480020781.01.T01:intron 65.0%
GGAAACCACGGCCATCGGGT+CGG - Chr4:48202212-48202231 None:intergenic 65.0%
TGGACCCGACCCGATGGCCG+TGG + Chr4:48202204-48202223 MsG0480020781.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 48202114 48206060 48202114 ID=MsG0480020781.01;Name=MsG0480020781.01
Chr4 mRNA 48202114 48206060 48202114 ID=MsG0480020781.01.T01;Parent=MsG0480020781.01;Name=MsG0480020781.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|0.83|0.71|7|0|258
Chr4 exon 48202114 48202249 48202114 ID=MsG0480020781.01.T01:exon:28175;Parent=MsG0480020781.01.T01
Chr4 exon 48203095 48203173 48203095 ID=MsG0480020781.01.T01:exon:28176;Parent=MsG0480020781.01.T01
Chr4 exon 48203281 48203383 48203281 ID=MsG0480020781.01.T01:exon:28177;Parent=MsG0480020781.01.T01
Chr4 exon 48203479 48203599 48203479 ID=MsG0480020781.01.T01:exon:28178;Parent=MsG0480020781.01.T01
Chr4 exon 48204861 48204949 48204861 ID=MsG0480020781.01.T01:exon:28179;Parent=MsG0480020781.01.T01
Chr4 exon 48205444 48205593 48205444 ID=MsG0480020781.01.T01:exon:28180;Parent=MsG0480020781.01.T01
Chr4 exon 48205962 48206060 48205962 ID=MsG0480020781.01.T01:exon:28181;Parent=MsG0480020781.01.T01
Chr4 CDS 48202114 48202249 48202114 ID=MsG0480020781.01.T01:cds;Parent=MsG0480020781.01.T01
Chr4 CDS 48203095 48203173 48203095 ID=MsG0480020781.01.T01:cds;Parent=MsG0480020781.01.T01
Chr4 CDS 48203281 48203383 48203281 ID=MsG0480020781.01.T01:cds;Parent=MsG0480020781.01.T01
Chr4 CDS 48203479 48203599 48203479 ID=MsG0480020781.01.T01:cds;Parent=MsG0480020781.01.T01
Chr4 CDS 48204861 48204949 48204861 ID=MsG0480020781.01.T01:cds;Parent=MsG0480020781.01.T01
Chr4 CDS 48205444 48205593 48205444 ID=MsG0480020781.01.T01:cds;Parent=MsG0480020781.01.T01
Chr4 CDS 48205962 48206060 48205962 ID=MsG0480020781.01.T01:cds;Parent=MsG0480020781.01.T01
Gene Sequence

>MsG0480020781.01.T01

ATGGCCGGCAAAAGTGGAACGACTCCGGCGACATACTCACCGTCTCTGACAACCGGTAAAAAGAAACCACTGCTTCTCAAGGAAGAAGATTGGACCCGACCCGATGGCCGTGGTTTCCACCAGTGTAGACCCGCTTTTTTCAGGACTGGTGCTGTGAATGCTGCATCAGGATCTGCTTATGCTGAGTTTGGAAATACCAAAGTCATTGTATCTGTGTTTGGGCCAAGAGAGAGCAAGAAGGCAATGATGTACAGTGATACAGGGCGTCTGAATTGCAATGTCAGCTATACAACGTTTTCAACTCCGGTTCGTGGACAGGGTTCAGACCACAAAGAGTACAGTTCGATGCTTCATAAAGCTTTGGAGGGTGCAATAATATTGGATTCTTTCCCCAAGACCACTGTGGATGTTTTTGCATTGGTGCTGGAATCTAGTGGCAGTGATCTCCCAGTTGTCATATCGGTTGCTAGTCTTGCCTTGGCAGATGCCGGAATACTGATGTATGACCTTGTCGCCTCAGTTTCAGTGTCATGTCTCGGTAAGAACCTCGTCATTGATCCTATATTTGAGGAGGAAAACTGCCAAGATGGAAGCTTAATGATTACTTGCATGCCTTCTCGCTATGAAATCACCCAACTGACAGTTACCGGCGAATGGTCCACCCCAAAGATTAATGAGGGGATGCAGCTGTGCTTGGATGCTTGTGCAAAGCTTGCTAAGATTATGAGGTCATGTTTGAAAGAATCTGCTTCTGATGCACAGGATGAAAGCTCTTAG
Protein sequence

>MsG0480020781.01.T01

MAGKSGTTPATYSPSLTTGKKKPLLLKEEDWTRPDGRGFHQCRPAFFRTGAVNAASGSAYAEFGNTKVIVSVFGPRESKKAMMYSDTGRLNCNVSYTTFSTPVRGQGSDHKEYSSMLHKALEGAIILDSFPKTTVDVFALVLESSGSDLPVVISVASLALADAGILMYDLVASVSVSCLGKNLVIDPIFEEENCQDGSLMITCMPSRYEITQLTVTGEWSTPKINEGMQLCLDACAKLAKIMRSCLKESASDAQDESS*