Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021180.01.T01 | AES89058.1 | 89.333 | 75 | 7 | 1 | 1 | 75 | 1 | 74 | 7.38E-39 | 133 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021180.01.T01 | G7JF00 | 89.333 | 75 | 7 | 1 | 1 | 75 | 1 | 74 | 3.52e-39 | 133 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180006100.01 | MsG0480021180.01 | 0.814243 | 1.701089e-51 | 1.135428e-48 |
| MsG0380013368.01 | MsG0480021180.01 | -0.805888 | 1.074184e-49 | 5.749112e-47 |
| MsG0380014767.01 | MsG0480021180.01 | 0.824520 | 7.720488e-54 | 6.854840e-51 |
| MsG0380016489.01 | MsG0480021180.01 | -0.802705 | 4.942633e-49 | 2.437698e-46 |
| MsG0280010244.01 | MsG0480021180.01 | 0.877709 | 5.055591e-69 | 2.567997e-65 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021180.01.T01 | MTR_4g068560 | 89.333 | 75 | 7 | 1 | 1 | 75 | 1 | 74 | 8.94e-43 | 133 |
| MsG0480021180.01.T01 | MTR_4g068453 | 69.333 | 75 | 19 | 2 | 1 | 75 | 1 | 71 | 3.67e-29 | 99.0 |
| MsG0480021180.01.T01 | MTR_1g034290 | 50.667 | 75 | 32 | 3 | 1 | 74 | 1 | 71 | 4.89e-18 | 70.9 |
| MsG0480021180.01.T01 | MTR_1g034270 | 43.243 | 74 | 34 | 1 | 1 | 74 | 1 | 66 | 7.49e-16 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021180.01.T01 | AT2G15960 | 40.244 | 82 | 37 | 2 | 1 | 75 | 1 | 77 | 8.25e-14 | 60.5 |
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAACCGTGTGTTTGCTTA+TGG | 0.279387 | 4:+54776072 | MsG0480021180.01.T01:CDS |
| CCATTGTAGAGGTATGGGTT+GGG | 0.390310 | 4:+54775894 | MsG0480021180.01.T01:CDS |
| AGGCAACAGAGGTGTCTCTT+GGG | 0.412772 | 4:-54776027 | None:intergenic |
| TTATGGATCGATTTGTTCCT+TGG | 0.422668 | 4:+54776089 | MsG0480021180.01.T01:CDS |
| TGGCTGCCATTGTAGAGGTA+TGG | 0.433651 | 4:+54775888 | MsG0480021180.01.T01:CDS |
| GAGGCAACAGAGGTGTCTCT+TGG | 0.459401 | 4:-54776028 | None:intergenic |
| CATTGTAGAGGTATGGGTTG+GGG | 0.470237 | 4:+54775895 | MsG0480021180.01.T01:CDS |
| GCCATTGTAGAGGTATGGGT+TGG | 0.477069 | 4:+54775893 | MsG0480021180.01.T01:CDS |
| GGCAACAGAGGTGTCTCTTG+GGG | 0.546169 | 4:-54776026 | None:intergenic |
| GGCTGCCATTGTAGAGGTAT+GGG | 0.559523 | 4:+54775889 | MsG0480021180.01.T01:CDS |
| AACAAAGGCTTGCTGTTGCT+AGG | 0.594023 | 4:-54775947 | None:intergenic |
| TGAAGAGTCAGAGGCAACAG+AGG | 0.597712 | 4:-54776038 | None:intergenic |
| ATTAATGGCTGCCATTGTAG+AGG | 0.614505 | 4:+54775883 | None:intergenic |
| TGACATAGTTGAAGAGTCAG+AGG | 0.626699 | 4:-54776047 | None:intergenic |
| CCCAACCCATACCTCTACAA+TGG | 0.629283 | 4:-54775894 | None:intergenic |
| CAAAACAAGAAGAAGAACGT+TGG | 0.651156 | 4:+54775995 | MsG0480021180.01.T01:CDS |
| TGACTCTTCAACTATGTCAG+AGG | 0.654740 | 4:+54776051 | MsG0480021180.01.T01:CDS |
| GCAACAGAGGTGTCTCTTGG+GGG | 0.674229 | 4:-54776025 | None:intergenic |
| ATTAACATATACTATCACCA+AGG | 0.710327 | 4:-54776106 | None:intergenic |
| TCGATCCATAAGCAAACACA+CGG | 0.710548 | 4:-54776077 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAAACAAGAAGAAGAACGT+TGG | + | Chr4:54775995-54776014 | MsG0480021180.01.T01:CDS | 35.0% | |
| ! | TTGTTTTCAAAAGCCGAACA+AGG | + | Chr4:54775965-54775984 | MsG0480021180.01.T01:CDS | 35.0% |
| !! | TGTTCGGCTTTTGAAAACAA+AGG | - | Chr4:54775965-54775984 | None:intergenic | 35.0% |
| !! | TTATGGATCGATTTGTTCCT+TGG | + | Chr4:54776089-54776108 | MsG0480021180.01.T01:CDS | 35.0% |
| TCGATCCATAAGCAAACACA+CGG | - | Chr4:54776080-54776099 | None:intergenic | 40.0% | |
| TGACATAGTTGAAGAGTCAG+AGG | - | Chr4:54776050-54776069 | None:intergenic | 40.0% | |
| TGACTCTTCAACTATGTCAG+AGG | + | Chr4:54776051-54776070 | MsG0480021180.01.T01:CDS | 40.0% | |
| ! | CATTGTAGAGGTATGGGTTG+GGG | + | Chr4:54775895-54775914 | MsG0480021180.01.T01:CDS | 45.0% |
| ! | CCATTGTAGAGGTATGGGTT+GGG | + | Chr4:54775894-54775913 | MsG0480021180.01.T01:CDS | 45.0% |
| !! | AACAAAGGCTTGCTGTTGCT+AGG | - | Chr4:54775950-54775969 | None:intergenic | 45.0% |
| !! | GTTTTGATCTGCGCCTTGTT+CGG | - | Chr4:54775981-54776000 | None:intergenic | 45.0% |
| CCCAACCCATACCTCTACAA+TGG | - | Chr4:54775897-54775916 | None:intergenic | 50.0% | |
| GGCAACCGTGTGTTTGCTTA+TGG | + | Chr4:54776072-54776091 | MsG0480021180.01.T01:CDS | 50.0% | |
| TGAAGAGTCAGAGGCAACAG+AGG | - | Chr4:54776041-54776060 | None:intergenic | 50.0% | |
| TGGCTGCCATTGTAGAGGTA+TGG | + | Chr4:54775888-54775907 | MsG0480021180.01.T01:CDS | 50.0% | |
| ! | GCCATTGTAGAGGTATGGGT+TGG | + | Chr4:54775893-54775912 | MsG0480021180.01.T01:CDS | 50.0% |
| ! | GGCTGCCATTGTAGAGGTAT+GGG | + | Chr4:54775889-54775908 | MsG0480021180.01.T01:CDS | 50.0% |
| !! | AGGCAACAGAGGTGTCTCTT+GGG | - | Chr4:54776030-54776049 | None:intergenic | 50.0% |
| !! | GAGGCAACAGAGGTGTCTCT+TGG | - | Chr4:54776031-54776050 | None:intergenic | 55.0% |
| !! | GCAACAGAGGTGTCTCTTGG+GGG | - | Chr4:54776028-54776047 | None:intergenic | 55.0% |
| !! | GGCAACAGAGGTGTCTCTTG+GGG | - | Chr4:54776029-54776048 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 54775887 | 54776114 | 54775887 | ID=MsG0480021180.01;Name=MsG0480021180.01 |
| Chr4 | mRNA | 54775887 | 54776114 | 54775887 | ID=MsG0480021180.01.T01;Parent=MsG0480021180.01;Name=MsG0480021180.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|75 |
| Chr4 | exon | 54775887 | 54776114 | 54775887 | ID=MsG0480021180.01.T01:exon:1103;Parent=MsG0480021180.01.T01 |
| Chr4 | CDS | 54775887 | 54776114 | 54775887 | ID=MsG0480021180.01.T01:cds;Parent=MsG0480021180.01.T01 |
| Gene Sequence |
| Protein sequence |
