AlfalfaGEDB | MsG0480021349.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021349.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021349.01.T01	GAU32288.1	93.269	104	6	1	4	106	3	106	4.67E-65	202

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021349.01.T01	O65695	83.333	108	15	2	1	106	1	107	3.94E-60	182

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004276.01	MsG0480021349.01	0.820771	5.750051e-53	4.592007e-50
MsG0180004493.01	MsG0480021349.01	0.817468	3.247492e-52	2.367069e-49
MsG0180005429.01	MsG0480021349.01	0.840172	1.020708e-57	1.442109e-54
MsG0480021349.01	MsG0680032060.01	0.823848	1.110730e-53	9.675592e-51
MsG0380017094.01	MsG0480021349.01	-0.822437	2.369991e-53	1.983705e-50
MsG0280009894.01	MsG0480021349.01	0.816308	5.913949e-52	4.175883e-49
MsG0280009918.01	MsG0480021349.01	0.808636	2.809300e-50	1.614540e-47

PPI

Gene1	Gene2	Type
MsG0380017838.01	MsG0480021349.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021349.01.T01	MTR_4g072190	93.396	106	1	1	1	106	1	100	2.18e-68	200
MsG0480021349.01.T01	MTR_3g117610	82.075	106	15	1	4	105	3	108	2.07e-61	183
MsG0480021349.01.T01	MTR_5g021820	71.698	106	28	1	1	106	1	104	1.21e-53	163
MsG0480021349.01.T01	MTR_3g113310	74.074	108	24	2	1	106	1	106	3.19e-52	159
MsG0480021349.01.T01	MTR_8g076040	72.897	107	26	2	1	106	1	105	4.26e-52	159
MsG0480021349.01.T01	MTR_6g077850	53.261	92	40	2	17	105	7	98	1.34e-29	102
MsG0480021349.01.T01	MTR_4g072880	52.941	85	35	2	20	103	8	88	5.36e-27	95.5
MsG0480021349.01.T01	MTR_4g072270	63.077	65	23	1	40	103	24	88	1.33e-25	92.0
MsG0480021349.01.T01	MTR_4g072310	62.687	67	24	1	40	105	24	90	2.40e-25	91.3
MsG0480021349.01.T01	MTR_4g072530	63.636	66	23	1	41	105	24	89	1.18e-24	89.4
MsG0480021349.01.T01	MTR_4g072500	63.636	66	23	1	41	105	24	89	1.24e-24	89.4
MsG0480021349.01.T01	MTR_4g072910	50.549	91	32	2	16	105	21	99	1.90e-24	89.4
MsG0480021349.01.T01	MTR_4g072640	63.077	65	23	1	42	105	25	89	2.61e-24	88.6
MsG0480021349.01.T01	MTR_4g072250	51.765	85	36	3	23	103	4	87	3.87e-24	88.2
MsG0480021349.01.T01	MTR_4g072220	51.163	86	38	2	21	105	8	90	7.92e-24	87.4
MsG0480021349.01.T01	MTR_4g072480	59.701	67	26	1	40	105	23	89	1.02e-23	87.0
MsG0480021349.01.T01	MTR_4g072730	61.194	67	25	1	40	105	23	89	1.52e-23	86.7
MsG0480021349.01.T01	MTR_4g072620	59.701	67	26	1	40	105	23	89	1.64e-23	86.7
MsG0480021349.01.T01	MTR_4g072860	51.724	87	37	2	20	105	8	90	2.44e-23	86.3
MsG0480021349.01.T01	MTR_4g072370	58.209	67	27	1	40	105	21	87	2.71e-23	85.9
MsG0480021349.01.T01	MTR_4g072570	58.209	67	27	1	40	105	23	89	3.92e-23	85.9
MsG0480021349.01.T01	MTR_5g008360	56.716	67	28	1	40	105	14	80	6.25e-23	85.1
MsG0480021349.01.T01	MTR_4g072240	59.375	64	25	1	41	103	25	88	6.38e-23	85.1
MsG0480021349.01.T01	MTR_4g072230	51.765	85	35	3	23	103	4	86	7.19e-23	85.1
MsG0480021349.01.T01	MTR_5g008370	59.701	67	26	1	41	106	24	90	7.35e-23	86.3
MsG0480021349.01.T01	MTR_4g072820	58.209	67	27	1	41	106	19	85	7.56e-23	84.7
MsG0480021349.01.T01	MTR_4g072560	50.000	90	38	3	18	105	13	97	1.15e-22	84.7
MsG0480021349.01.T01	MTR_4g072980	50.549	91	33	3	18	106	13	93	1.19e-22	84.7
MsG0480021349.01.T01	MTR_4g072260	48.837	86	40	2	21	105	8	90	1.45e-22	84.3
MsG0480021349.01.T01	MTR_3g117640	60.294	68	25	2	41	106	33	100	1.71e-22	84.3
MsG0480021349.01.T01	MTR_3g084150	56.923	65	27	1	40	103	23	87	2.27e-22	84.0
MsG0480021349.01.T01	MTR_4g072660	60.000	65	25	1	40	103	18	82	2.37e-22	83.6
MsG0480021349.01.T01	MTR_3g084180	56.923	65	27	1	40	103	23	87	2.86e-22	83.6
MsG0480021349.01.T01	MTR_3g084210	59.701	67	26	1	40	105	23	89	3.48e-22	83.2
MsG0480021349.01.T01	MTR_3g084250	58.209	67	27	1	40	105	23	89	3.52e-22	83.2
MsG0480021349.01.T01	MTR_3g084240	47.619	84	41	2	23	103	4	87	3.56e-22	83.2
MsG0480021349.01.T01	MTR_4g072890	48.837	86	40	2	21	105	8	90	3.74e-22	83.2
MsG0480021349.01.T01	MTR_4g072330	56.061	66	28	1	41	105	22	87	4.88e-22	82.8
MsG0480021349.01.T01	MTR_8g461410	55.385	65	28	1	40	103	23	87	4.94e-22	82.8
MsG0480021349.01.T01	MTR_4g072540	56.923	65	27	1	40	103	19	83	5.05e-22	82.8
MsG0480021349.01.T01	MTR_4g072850	58.462	65	26	1	40	103	25	89	5.21e-22	82.8
MsG0480021349.01.T01	MTR_3g084170	58.462	65	26	1	40	103	23	87	5.33e-22	82.8
MsG0480021349.01.T01	MTR_3g084220	58.462	65	26	1	40	103	23	87	6.77e-22	82.4
MsG0480021349.01.T01	MTR_4g072870	45.556	90	44	2	17	105	5	90	8.38e-22	82.4
MsG0480021349.01.T01	MTR_4g072300	56.250	64	27	1	41	103	25	88	9.55e-22	82.0
MsG0480021349.01.T01	MTR_4g072680	56.716	67	28	1	40	105	23	89	1.54e-21	81.6
MsG0480021349.01.T01	MTR_3g110038	56.923	65	27	1	40	103	23	87	1.57e-21	81.6
MsG0480021349.01.T01	MTR_3g117630	58.462	65	26	1	40	103	23	87	2.45e-21	81.3
MsG0480021349.01.T01	MTR_4g072460	56.716	67	28	1	40	105	23	89	2.62e-21	81.3
MsG0480021349.01.T01	MTR_3g084230	58.209	67	27	1	40	105	23	89	2.77e-21	80.9
MsG0480021349.01.T01	MTR_3g084160	56.716	67	28	1	40	105	23	89	3.15e-21	80.9
MsG0480021349.01.T01	MTR_4g072580	56.923	65	27	1	40	103	18	82	3.32e-21	80.5
MsG0480021349.01.T01	MTR_4g072610	55.224	67	29	1	40	105	17	83	3.49e-21	80.5
MsG0480021349.01.T01	MTR_4g072670	55.224	67	29	1	40	105	19	85	3.64e-21	80.5
MsG0480021349.01.T01	MTR_4g072590	53.731	67	30	1	40	105	18	84	4.98e-21	80.1
MsG0480021349.01.T01	MTR_4g072490	46.591	88	36	2	17	103	5	82	7.29e-21	79.7
MsG0480021349.01.T01	MTR_4g072740	46.667	90	37	2	17	105	5	84	9.58e-21	79.3
MsG0480021349.01.T01	MTR_1g063950	50.725	69	34	0	33	101	39	107	1.09e-20	80.5
MsG0480021349.01.T01	MTR_4g072720	53.846	65	29	1	40	103	19	83	1.47e-20	79.0
MsG0480021349.01.T01	MTR_8g461420	56.716	67	28	1	40	105	23	89	1.66e-20	79.0
MsG0480021349.01.T01	MTR_4g050700	57.812	64	26	1	41	103	25	88	1.70e-20	79.0
MsG0480021349.01.T01	MTR_2g012410	57.812	64	26	1	41	103	25	88	1.70e-20	79.0
MsG0480021349.01.T01	MTR_3g110045	60.000	65	25	1	40	103	82	146	1.95e-20	80.5
MsG0480021349.01.T01	MTR_3g084190	55.224	67	29	1	40	105	23	89	2.01e-20	79.0
MsG0480021349.01.T01	MTR_4g072510	53.846	65	29	1	40	103	19	83	2.02e-20	78.6
MsG0480021349.01.T01	MTR_4g072830	55.385	65	28	1	40	103	21	85	2.40e-20	78.6
MsG0480021349.01.T01	MTR_3g110042	55.224	67	29	1	40	105	23	89	2.77e-20	78.6
MsG0480021349.01.T01	MTR_4g072940	54.688	64	28	1	40	102	55	118	2.80e-20	79.3
MsG0480021349.01.T01	MTR_4g072690	55.385	65	28	1	40	103	18	82	4.92e-20	77.8
MsG0480021349.01.T01	MTR_4g072630	52.308	65	30	1	40	103	19	83	6.36e-20	77.4
MsG0480021349.01.T01	MTR_4g072470	46.667	90	37	2	17	105	5	84	6.90e-20	77.4
MsG0480021349.01.T01	MTR_3g084260	56.250	64	27	1	41	103	25	88	7.25e-20	77.4
MsG0480021349.01.T01	MTR_4g072465	53.846	65	29	1	40	103	19	83	7.73e-20	77.0
MsG0480021349.01.T01	MTR_7g101740	48.611	72	37	0	33	104	31	102	1.39e-19	77.4
MsG0480021349.01.T01	MTR_4g072550	57.812	64	26	1	41	103	35	98	1.56e-19	77.0
MsG0480021349.01.T01	MTR_4g072600	53.731	67	30	1	40	105	18	84	1.58e-19	76.3
MsG0480021349.01.T01	MTR_1g024085	44.944	89	47	1	17	105	14	100	1.89e-19	77.8
MsG0480021349.01.T01	MTR_3g110032	54.688	64	28	1	41	103	25	88	1.97e-19	76.3
MsG0480021349.01.T01	MTR_4g072650	52.308	65	30	1	40	103	17	81	3.72e-19	75.5
MsG0480021349.01.T01	MTR_4g072770	55.385	65	28	1	42	105	15	79	7.15e-19	74.7
MsG0480021349.01.T01	MTR_4g072450	53.846	65	29	1	40	103	18	82	9.77e-19	74.3
MsG0480021349.01.T01	MTR_4g072380	46.591	88	37	2	17	103	5	83	2.72e-18	73.2
MsG0480021349.01.T01	MTR_2g043960	52.174	69	33	0	37	105	71	139	4.02e-18	74.7
MsG0480021349.01.T01	MTR_1g094750	44.872	78	40	1	24	101	35	109	6.17e-18	73.2
MsG0480021349.01.T01	MTR_1g061570	40.260	77	46	0	25	101	2	78	1.09e-17	72.8
MsG0480021349.01.T01	MTR_4g124750	42.857	91	48	1	20	106	43	133	2.42e-17	72.8
MsG0480021349.01.T01	MTR_8g461210	36.538	104	58	2	3	106	4	99	6.44e-17	70.5
MsG0480021349.01.T01	MTR_5g008350	51.724	58	27	1	40	96	18	75	6.70e-17	69.3
MsG0480021349.01.T01	MTR_1g009150	49.231	65	33	0	41	105	70	134	1.08e-16	71.6
MsG0480021349.01.T01	MTR_3g109160	40.230	87	49	1	19	105	49	132	1.80e-16	70.9
MsG0480021349.01.T01	MTR_4g072930	55.224	67	25	2	42	103	41	107	3.12e-16	68.6
MsG0480021349.01.T01	MTR_4g072750	58.333	48	20	0	41	88	26	73	4.16e-16	67.8
MsG0480021349.01.T01	MTR_7g118010	41.250	80	39	2	28	105	15	88	1.21e-15	67.0
MsG0480021349.01.T01	MTR_3g110035	57.143	56	23	1	51	105	80	135	1.79e-15	67.4
MsG0480021349.01.T01	MTR_3g092220	40.260	77	44	1	31	105	22	98	1.91e-15	66.6
MsG0480021349.01.T01	MTR_7g104950	45.902	61	33	0	45	105	53	113	2.37e-15	67.0
MsG0480021349.01.T01	MTR_4g025830	44.776	67	34	1	42	105	79	145	2.94e-15	67.4
MsG0480021349.01.T01	MTR_1g059770	39.189	74	45	0	33	106	40	113	3.27e-15	66.6
MsG0480021349.01.T01	MTR_7g104940	45.902	61	33	0	45	105	53	113	4.15e-15	66.2
MsG0480021349.01.T01	MTR_4g072290	55.769	52	22	1	55	105	25	76	4.22e-15	65.1
MsG0480021349.01.T01	MTR_2g095430	43.478	69	35	1	30	94	33	101	7.44e-15	65.9
MsG0480021349.01.T01	MTR_3g084200	54.348	46	21	0	40	85	23	68	8.87e-15	64.7
MsG0480021349.01.T01	MTR_4g072160	37.113	97	54	1	9	105	11	100	1.08e-14	65.5
MsG0480021349.01.T01	MTR_0388s0020	43.478	69	36	1	40	105	73	141	1.80e-14	65.1
MsG0480021349.01.T01	MTR_4g005320	43.750	64	36	0	42	105	70	133	1.89e-14	65.5
MsG0480021349.01.T01	MTR_4g057800	42.697	89	44	3	10	94	9	94	3.08e-14	64.3
MsG0480021349.01.T01	MTR_4g057810	40.659	91	50	3	13	101	11	99	1.24e-13	62.4
MsG0480021349.01.T01	MTR_4g072280	55.814	43	19	0	41	83	25	67	3.34e-13	60.1
MsG0480021349.01.T01	MTR_8g026730	42.424	66	36	1	42	105	27	92	3.66e-13	60.8
MsG0480021349.01.T01	MTR_2g066830	35.385	65	42	0	42	106	58	122	3.10e-12	59.3
MsG0480021349.01.T01	MTR_4g021810	43.077	65	36	1	42	105	26	90	6.84e-12	57.4
MsG0480021349.01.T01	MTR_3g098970	42.105	57	33	0	42	98	53	109	1.23e-11	57.8
MsG0480021349.01.T01	MTR_7g101920	46.429	56	28	1	41	94	34	89	2.02e-11	56.2
MsG0480021349.01.T01	MTR_4g005310	43.548	62	33	1	42	101	62	123	5.54e-11	56.2
MsG0480021349.01.T01	MTR_8g101520	35.294	102	58	1	1	94	1	102	7.66e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021349.01.T01	AT4G34760	83.333	108	15	2	1	106	1	107	4.02e-61	182
MsG0480021349.01.T01	AT2G21220	78.095	105	21	1	1	105	1	103	9.06e-57	171
MsG0480021349.01.T01	AT4G38860	78.505	107	20	2	1	106	1	105	2.35e-55	167
MsG0480021349.01.T01	AT2G16580	75.229	109	23	2	1	106	1	108	6.35e-55	166
MsG0480021349.01.T01	AT1G75580	76.190	105	21	2	6	106	4	108	1.50e-53	163
MsG0480021349.01.T01	AT1G19830	66.087	115	28	3	1	106	1	113	2.02e-48	150
MsG0480021349.01.T01	AT4G36110	70.755	106	29	1	1	106	1	104	8.83e-48	148
MsG0480021349.01.T01	AT2G18010	64.286	112	34	1	1	106	1	112	8.76e-46	143
MsG0480021349.01.T01	AT5G66260	66.292	89	28	1	17	105	11	97	3.11e-35	116
MsG0480021349.01.T01	AT2G21210	48.889	90	41	3	17	103	5	92	2.02e-24	89.4
MsG0480021349.01.T01	AT4G34770	61.765	68	23	2	41	105	34	101	5.96e-24	88.2
MsG0480021349.01.T01	AT2G21210	48.889	90	41	3	17	103	72	159	7.90e-24	89.7
MsG0480021349.01.T01	AT4G38840	64.615	65	21	2	41	103	32	96	1.45e-23	87.0
MsG0480021349.01.T01	AT4G13790	55.714	70	28	2	40	106	23	92	1.44e-22	84.3
MsG0480021349.01.T01	AT3G51200	48.387	93	40	3	15	105	17	103	1.47e-22	84.7
MsG0480021349.01.T01	AT4G34810	52.747	91	36	4	18	103	26	114	1.65e-22	85.1
MsG0480021349.01.T01	AT5G18080	47.253	91	35	3	18	106	11	90	2.23e-21	81.3
MsG0480021349.01.T01	AT4G34800	60.938	64	21	2	42	101	21	84	2.28e-21	81.3
MsG0480021349.01.T01	AT4G38825	48.148	81	40	2	27	105	8	88	2.95e-21	80.9
MsG0480021349.01.T01	AT4G38850	47.312	93	31	3	18	106	11	89	3.79e-21	80.5
MsG0480021349.01.T01	AT2G21200	58.209	67	26	2	41	105	20	86	4.30e-21	80.5
MsG0480021349.01.T01	AT5G18030	56.716	67	27	2	42	106	22	88	4.35e-21	80.5
MsG0480021349.01.T01	AT4G34780	54.930	71	28	2	40	106	26	96	6.96e-21	80.5
MsG0480021349.01.T01	AT5G18020	60.938	64	23	2	42	103	24	87	7.37e-21	80.1
MsG0480021349.01.T01	AT5G18010	46.154	91	36	3	18	106	11	90	1.09e-20	79.3
MsG0480021349.01.T01	AT5G18050	55.224	67	28	2	42	106	24	90	1.34e-20	79.3
MsG0480021349.01.T01	AT3G03830	57.971	69	25	2	42	106	24	92	1.57e-20	79.3
MsG0480021349.01.T01	AT5G18060	58.462	65	25	2	43	105	25	89	1.74e-20	79.0
MsG0480021349.01.T01	AT3G43120	46.512	86	45	1	21	106	59	143	1.11e-19	79.0
MsG0480021349.01.T01	AT3G03820	54.286	70	28	2	41	106	26	95	2.12e-19	76.3
MsG0480021349.01.T01	AT3G03840	55.072	69	28	2	41	106	26	94	2.96e-19	75.9
MsG0480021349.01.T01	AT3G20220	49.180	61	30	1	42	101	47	107	4.56e-19	76.3
MsG0480021349.01.T01	AT1G75590	40.659	91	54	0	9	99	9	99	6.50e-19	76.6
MsG0480021349.01.T01	AT2G37030	52.459	61	29	0	38	98	45	105	1.41e-18	75.1
MsG0480021349.01.T01	AT5G20810	53.846	65	30	0	37	101	74	138	1.50e-18	76.3
MsG0480021349.01.T01	AT4G34790	56.923	65	25	2	42	103	39	103	2.94e-18	73.9
MsG0480021349.01.T01	AT4G34750	39.604	101	54	2	8	105	4	100	3.12e-18	75.1
MsG0480021349.01.T01	AT4G34750	39.604	101	54	2	8	105	4	100	3.12e-18	75.1
MsG0480021349.01.T01	AT5G20810	53.846	65	30	0	37	101	74	138	4.00e-18	75.5
MsG0480021349.01.T01	AT3G03850	55.224	67	27	2	43	106	26	92	6.53e-18	72.4
MsG0480021349.01.T01	AT1G19840	41.573	89	51	1	17	105	17	104	4.52e-17	72.0
MsG0480021349.01.T01	AT5G10990	40.964	83	48	1	17	99	17	98	8.05e-17	71.2
MsG0480021349.01.T01	AT3G60690	46.575	73	36	1	36	105	82	154	1.02e-15	68.9
MsG0480021349.01.T01	AT1G56150	31.373	102	58	1	17	106	1	102	1.96e-15	66.6
MsG0480021349.01.T01	AT2G28085	47.059	68	32	1	31	94	44	111	2.18e-15	67.0
MsG0480021349.01.T01	AT4G31320	45.312	64	35	0	42	105	80	143	2.63e-15	68.2
MsG0480021349.01.T01	AT3G61900	45.588	68	34	1	41	105	29	96	3.10e-15	67.0
MsG0480021349.01.T01	AT2G45210	41.892	74	40	1	35	105	72	145	5.47e-15	67.0
MsG0480021349.01.T01	AT2G24400	46.875	64	34	0	42	105	64	127	5.57e-15	67.0
MsG0480021349.01.T01	AT3G53250	42.466	73	37	2	26	98	38	105	5.67e-14	63.2
MsG0480021349.01.T01	AT3G53250	37.500	96	55	2	3	98	46	136	1.03e-13	63.5
MsG0480021349.01.T01	AT3G12830	40.000	65	39	0	42	106	50	114	1.47e-13	62.4
MsG0480021349.01.T01	AT2G46690	42.647	68	36	1	41	105	23	90	1.77e-13	62.0
MsG0480021349.01.T01	AT1G16510	40.000	65	39	0	42	106	54	118	4.33e-13	61.6
MsG0480021349.01.T01	AT5G53590	44.286	70	34	1	41	105	45	114	4.70e-13	61.2
MsG0480021349.01.T01	AT1G79130	40.000	65	39	0	42	106	48	112	6.67e-13	60.8
MsG0480021349.01.T01	AT2G36210	38.961	77	45	2	31	105	42	118	1.54e-12	59.7
MsG0480021349.01.T01	AT4G09530	43.750	64	32	2	42	101	28	91	3.33e-12	58.2
MsG0480021349.01.T01	AT4G00880	42.424	66	36	1	42	105	28	93	6.10e-12	58.2
MsG0480021349.01.T01	AT1G76190	39.683	63	37	1	44	106	23	84	8.06e-12	57.8
MsG0480021349.01.T01	AT4G12410	41.791	67	36	1	42	105	76	142	8.63e-11	55.8
MsG0480021349.01.T01	AT1G20470	42.857	63	35	1	44	106	25	86	9.80e-11	55.5

Find 9 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTCAAGCTGAAGAAGAATT+TGG	0.180585	4:+56924728	MsG0480021349.01.T01:CDS
GTTGGAGTATTTCTTGATAA+TGG	0.303731	4:-56924510	None:intergenic
TAGACAGGAAAATGACCTTT+TGG	0.421755	4:-56924632	None:intergenic
CTCAAAAGATGTTCAAGCTT+AGG	0.460632	4:+56924566	MsG0480021349.01.T01:CDS
TGATGATAATGAACTACCTA+TGG	0.494980	4:+56924604	MsG0480021349.01.T01:CDS
CGAAGTAGTGATTGAAACTC+AGG	0.507028	4:-56924707	None:intergenic
CTACCTATGGATGTTCCAAA+AGG	0.526934	4:+56924617	MsG0480021349.01.T01:CDS
TTGGAACAATATATCTACTA+CGG	0.543081	4:-56924667	None:intergenic
CTTCTTGACAAGGAATAGTG+AGG	0.705041	4:-56924769	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GGATGAGTTAAAAAAGATAT+TGG	-	Chr4:56924689-56924708	None:intergenic	25.0%
!	TTGGAACAATATATCTACTA+CGG	-	Chr4:56924670-56924689	None:intergenic	25.0%
!!!	ATTTGGTTTTGATCATGATA+TGG	+	Chr4:56924745-56924764	MsG0480021349.01.T01:CDS	25.0%
!!!	TTTGGTTTTGATCATGATAT+GGG	+	Chr4:56924746-56924765	MsG0480021349.01.T01:CDS	25.0%
	TGAAAAACAACTTCTTGACA+AGG	-	Chr4:56924782-56924801	None:intergenic	30.0%
!	GTTGGAGTATTTCTTGATAA+TGG	-	Chr4:56924513-56924532	None:intergenic	30.0%
!	TGATGATAATGAACTACCTA+TGG	+	Chr4:56924604-56924623	MsG0480021349.01.T01:CDS	30.0%
!	TGTTTCAAAACTGCATTTTG+AGG	-	Chr4:56924542-56924561	None:intergenic	30.0%
	CTCAAAAGATGTTCAAGCTT+AGG	+	Chr4:56924566-56924585	MsG0480021349.01.T01:CDS	35.0%
!	CTAAGCTTGAACATCTTTTG+AGG	-	Chr4:56924568-56924587	None:intergenic	35.0%
!	TAGACAGGAAAATGACCTTT+TGG	-	Chr4:56924635-56924654	None:intergenic	35.0%
!!!	TGCATTTTGAGGTAGTTTGT+TGG	-	Chr4:56924531-56924550	None:intergenic	35.0%
	CGAAGTAGTGATTGAAACTC+AGG	-	Chr4:56924710-56924729	None:intergenic	40.0%
	CGTCAAGCTGAAGAAGAATT+TGG	+	Chr4:56924728-56924747	MsG0480021349.01.T01:CDS	40.0%
	CTACCTATGGATGTTCCAAA+AGG	+	Chr4:56924617-56924636	MsG0480021349.01.T01:CDS	40.0%
	CTTCTTGACAAGGAATAGTG+AGG	-	Chr4:56924772-56924791	None:intergenic	40.0%
!!!	TGACCTTTTGGAACATCCAT+AGG	-	Chr4:56924623-56924642	None:intergenic	40.0%
!!	GGTCATTTTCCTGTCTACGT+AGG	+	Chr4:56924638-56924657	MsG0480021349.01.T01:CDS	45.0%
!	CGGTTTTCACCTACGTAGAC+AGG	-	Chr4:56924650-56924669	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	56924506	56924826	56924506	ID=MsG0480021349.01;Name=MsG0480021349.01
Chr4	mRNA	56924506	56924826	56924506	ID=MsG0480021349.01.T01;Parent=MsG0480021349.01;Name=MsG0480021349.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|106
Chr4	exon	56924506	56924826	56924506	ID=MsG0480021349.01.T01:exon:1354;Parent=MsG0480021349.01.T01
Chr4	CDS	56924506	56924826	56924506	ID=MsG0480021349.01.T01:cds;Parent=MsG0480021349.01.T01

Gene Sequence	>MsG0480021349.01.T01 ATGGCCATTATCAAGAAATACTCCAACAAACTACCTCAAAATGCAGTTTTGAAACAAATCCTCAAAAGATGTTCAAGCTTAGGAAAGAAAAATGAGTATGATGATAATGAACTACCTATGGATGTTCCAAAAGGTCATTTTCCTGTCTACGTAGGTGAAAACCGTAGTAGATATATTGTTCCAATATCTTTTTTAACTCATCCTGAGTTTCAATCACTACTTCGTCAAGCTGAAGAAGAATTTGGTTTTGATCATGATATGGGCCTCACTATTCCTTGTCAAGAAGTTGTTTTTCAATCTCTAACATCAATGATTAGATGA
Protein sequence	>MsG0480021349.01.T01 MAIIKKYSNKLPQNAVLKQILKRCSSLGKKNEYDDNELPMDVPKGHFPVYVGENRSRYIVPISFLTHPEFQSLLRQAEEEFGFDHDMGLTIPCQEVVFQSLTSMIR*