AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021378.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021378.01.T01 MTR_4g062570 98.876 356 4 0 1 356 1 356 0.0 717
MsG0480021378.01.T01 MTR_4g072780 98.596 356 5 0 1 356 1 356 0.0 716
MsG0480021378.01.T01 MTR_4g062550 98.034 356 7 0 1 356 1 356 0.0 713
MsG0480021378.01.T01 MTR_4g032360 98.315 356 6 0 1 356 1 356 0.0 712
MsG0480021378.01.T01 MTR_5g022720 87.054 224 6 1 133 356 73 273 5.27e-136 388
MsG0480021378.01.T01 MTR_8g088860 41.737 357 205 3 1 354 1 357 1.30e-102 306
MsG0480021378.01.T01 MTR_8g088880 40.517 348 205 2 1 346 1 348 4.20e-96 290
MsG0480021378.01.T01 MTR_8g088890 39.867 301 179 2 29 327 1 301 2.49e-83 256
MsG0480021378.01.T01 MTR_7g097000 22.616 451 214 11 17 334 55 503 4.80e-23 100
MsG0480021378.01.T01 MTR_7g033690 88.889 36 4 0 31 66 1 36 3.06e-15 70.9
MsG0480021378.01.T01 MTR_7g096970 28.750 160 111 2 196 353 365 523 1.71e-13 72.0
MsG0480021378.01.T01 MTR_7g096970 25.389 193 102 5 30 180 61 253 7.46e-12 67.0
MsG0480021378.01.T01 MTR_2g093550 30.061 163 104 6 176 333 50 207 4.16e-12 65.5
MsG0480021378.01.T01 MTR_2g093630 32.374 139 86 5 200 333 61 196 5.55e-12 65.5
MsG0480021378.01.T01 MTR_2g093350 27.778 180 119 7 181 355 281 454 1.24e-11 65.9
MsG0480021378.01.T01 MTR_2g093620 32.143 140 87 5 200 334 296 432 1.85e-11 65.5
MsG0480021378.01.T01 MTR_2g093590 29.012 162 104 7 200 355 295 451 2.32e-11 65.1
MsG0480021378.01.T01 MTR_1g072600 28.671 143 95 4 196 335 422 560 2.92e-11 65.1
MsG0480021378.01.T01 MTR_2g093390 28.395 162 105 7 200 355 295 451 5.46e-11 63.9
MsG0480021378.01.T01 MTR_1g072610 28.671 143 95 4 196 335 391 529 7.39e-11 63.9
MsG0480021378.01.T01 MTR_4g060780 32.743 113 73 3 223 333 307 418 7.85e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021378.01.T01 AT1G07750 79.775 356 72 0 1 356 1 356 0.0 597
MsG0480021378.01.T01 AT2G28680 79.213 356 74 0 1 356 1 356 0.0 593
MsG0480021378.01.T01 AT1G03890 24.939 409 243 11 5 354 44 447 1.49e-32 126
MsG0480021378.01.T01 AT1G03880 23.214 392 235 9 12 339 42 431 1.28e-28 115
MsG0480021378.01.T01 AT5G44120 22.475 396 234 8 12 336 48 441 7.11e-25 105
MsG0480021378.01.T01 AT5G44120 23.780 328 203 7 54 336 12 337 1.44e-20 92.0
MsG0480021378.01.T01 AT4G28520 25.914 301 180 7 78 336 193 492 7.20e-19 88.2
MsG0480021378.01.T01 AT4G28520 30.714 140 96 1 197 336 283 421 1.70e-15 77.8
MsG0480021378.01.T01 AT2G28490 23.219 379 221 12 42 355 122 495 2.52e-15 77.4
MsG0480021378.01.T01 AT5G44120 24.123 228 145 4 136 336 28 254 8.19e-14 71.6

Find 106 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCCATTCTACTTTAATT+TGG 0.172406 4:+57251460 None:intergenic
CCTTTCTTATGCTCCTCTTT+AGG 0.239793 4:+57252097 None:intergenic
GGTCCTAACGGAATCTTTAC+TGG 0.243709 4:-57252241 MsG0480021378.01.T01:CDS
ATAGTTATATTTCTTCCTAT+TGG 0.254736 4:-57251272 MsG0480021378.01.T01:three_prime_UTR
CGGAAGAGCTTCTCAACTTC+TGG 0.259062 4:+57251520 None:intergenic
ACAACCTTCTCTTCTGATTC+AGG 0.286854 4:+57252412 None:intergenic
AGAGAAGGTTGTCGCAATTA+AGG 0.292186 4:-57252401 MsG0480021378.01.T01:CDS
GATGCTTTAGCACTCCCTTT+TGG 0.316918 4:-57252373 MsG0480021378.01.T01:CDS
TAGAGGGAGCGGCCGTGTTC+AGG 0.324622 4:-57251879 MsG0480021378.01.T01:CDS
CTTCCTTTGGTTGGTGAGGT+TGG 0.336501 4:-57251992 MsG0480021378.01.T01:CDS
AAGAAATATAACTATATCTA+AGG 0.336897 4:+57251280 None:intergenic
AGGCATGGCTTTGAACTGTT+TGG 0.348039 4:-57252077 MsG0480021378.01.T01:CDS
TTGGTTGGTGAGGTTGGTTT+AGG 0.350208 4:-57251986 MsG0480021378.01.T01:CDS
ATTTGGAGGAGGGAAGAAAA+TGG 0.351352 4:+57251477 None:intergenic
TGTTGATGGTAAGAGAGTTT+TGG 0.351818 4:-57251849 MsG0480021378.01.T01:CDS
GTTCAGGTTGTTGGTGTTGA+TGG 0.356237 4:-57251863 MsG0480021378.01.T01:CDS
AGCGGCCGTGTTCAGGTTGT+TGG 0.361324 4:-57251872 MsG0480021378.01.T01:CDS
AACAATGTGAAGACTCTTGT+TGG 0.387512 4:-57252175 MsG0480021378.01.T01:CDS
CTCTTGCGATTCTGCTCTAC+AGG 0.401819 4:-57251915 MsG0480021378.01.T01:CDS
GCTGACCTTGTGAGGATTGA+TGG 0.407307 4:-57251962 MsG0480021378.01.T01:CDS
AAGAAGTTGATTGTCTCAAT+TGG 0.407945 4:-57251353 MsG0480021378.01.T01:three_prime_UTR
AAGCAAAATTTGTATCCAAT+AGG 0.410174 4:+57251257 None:intergenic
ACCAACCAAAGGAAGGTTCT+TGG 0.412307 4:+57252000 None:intergenic
AAGGTTGCTTATGTTCTTCA+AGG 0.412348 4:-57253296 MsG0480021378.01.T01:intron
GAACACCAAGAACCTTCCTT+TGG 0.413394 4:-57252005 MsG0480021378.01.T01:CDS
CTTCCACACCGAAGAACTTC+CGG 0.415818 4:+57251585 None:intergenic
TCCATTCTACTTTAATTTGG+AGG 0.417794 4:+57251463 None:intergenic
CACCATGTCACAACACCAAA+AGG 0.420280 4:+57252358 None:intergenic
TTGGAAAGCAATCTGCTAAA+GGG 0.426822 4:-57252156 MsG0480021378.01.T01:CDS
GGAAGATCCATGTGCTCTCC+TGG 0.428535 4:-57251941 MsG0480021378.01.T01:CDS
CAGGTTACTTACATTGTTAG+AGG 0.431822 4:-57251896 MsG0480021378.01.T01:CDS
AAGTTAGCTCTTCAGAAGAA+TGG 0.434931 4:-57253350 MsG0480021378.01.T01:CDS
ACACATGGCCGGAAGTTCTT+CGG 0.439706 4:-57251593 MsG0480021378.01.T01:CDS
CTGAGTTTGTGGGAAGAGCT+TGG 0.442265 4:-57252210 MsG0480021378.01.T01:CDS
TGAGTTTGTGGGAAGAGCTT+GGG 0.443495 4:-57252209 MsG0480021378.01.T01:CDS
ATTCTTCTGAAGAGCTAACT+TGG 0.448714 4:+57253352 None:intergenic
TACACTTTCTTAGCCGATTG+AGG 0.457155 4:+57253452 None:intergenic
CATGGCTTTGAACTGTTTGG+AGG 0.461927 4:-57252074 MsG0480021378.01.T01:CDS
ACCAAGAACCTTCCTTTGGT+TGG 0.462283 4:-57252001 MsG0480021378.01.T01:CDS
TCCTCCAAATTAAAGTAGAA+TGG 0.462317 4:-57251464 MsG0480021378.01.T01:exon
TATCGATCTTTCTCCTCAAT+CGG 0.467279 4:-57253465 MsG0480021378.01.T01:CDS
TTATGCTCCTCTTTAGGCTG+AGG 0.472213 4:+57252103 None:intergenic
GAGTTGGTTGTTCTGTTCCT+CGG 0.474191 4:-57252316 MsG0480021378.01.T01:CDS
GTTGGAAAGCAATCTGCTAA+AGG 0.477335 4:-57252157 MsG0480021378.01.T01:CDS
GATACAGGAAGTGGAGTTGC+TGG 0.481664 4:-57252445 MsG0480021378.01.T01:intron
GAACCTTCCTTTGGTTGGTG+AGG 0.484774 4:-57251996 MsG0480021378.01.T01:CDS
TTGGAGACAACTTTGAAGGC+TGG 0.487933 4:-57251830 MsG0480021378.01.T01:CDS
GAACTGTTTGGAGGCACCAT+TGG 0.492260 4:-57252065 MsG0480021378.01.T01:CDS
AAAGGGATTGTGAAGCTTGA+CGG 0.493208 4:-57252139 MsG0480021378.01.T01:CDS
TCGAAGATTGCTGACAATGA+TGG 0.493938 4:-57251773 MsG0480021378.01.T01:CDS
ATTCTACTTTAATTTGGAGG+AGG 0.504281 4:+57251466 None:intergenic
CGGAAGTTCTTCGGTGTGGA+AGG 0.504760 4:-57251584 MsG0480021378.01.T01:CDS
GCTATCGCCTACGGTTCTGC+AGG 0.509869 4:-57251560 MsG0480021378.01.T01:CDS
GCTCCTCTTTAGGCTGAGGA+AGG 0.513125 4:+57252107 None:intergenic
CCGGCCATGTGTGTAAATAC+AGG 0.515763 4:+57251604 None:intergenic
AAGCCTTCCTCAGCCTAAAG+AGG 0.519673 4:-57252110 MsG0480021378.01.T01:CDS
TTGAAGAACATAAGCAACCT+TGG 0.522383 4:+57253298 None:intergenic
ACTTACATTGTTAGAGGGAG+CGG 0.529227 4:-57251890 MsG0480021378.01.T01:CDS
AATCCAGTAAAGATTCCGTT+AGG 0.529277 4:+57252238 None:intergenic
GAGATTTGTTCATTGTGCCA+AGG 0.529346 4:-57251808 MsG0480021378.01.T01:CDS
CAGTCCTGTATTTACACACA+TGG 0.531226 4:-57251608 MsG0480021378.01.T01:CDS
AAAGTGTACGGTGGCGATGG+TGG 0.536514 4:-57253437 MsG0480021378.01.T01:CDS
TTAAAGACATATCAAAATAG+AGG 0.537075 4:+57251317 None:intergenic
TTGGATGTTGATATTAAGAA+TGG 0.538544 4:-57252046 MsG0480021378.01.T01:CDS
GAGGTATTCTGATTCTTCCA+AGG 0.542124 4:-57253315 MsG0480021378.01.T01:CDS
GTGGAAGGCGCTATCGCCTA+CGG 0.542651 4:-57251569 MsG0480021378.01.T01:CDS
AGGTTACTTACATTGTTAGA+GGG 0.546023 4:-57251895 MsG0480021378.01.T01:CDS
ACCATGTCACAACACCAAAA+GGG 0.547146 4:+57252359 None:intergenic
GTTTAGGAGCTGACCTTGTG+AGG 0.547750 4:-57251970 MsG0480021378.01.T01:CDS
TTCTACTTTAATTTGGAGGA+GGG 0.549544 4:+57251467 None:intergenic
CCTAAAGAGGAGCATAAGAA+AGG 0.550642 4:-57252097 MsG0480021378.01.T01:CDS
ATGGTGGATCGTATTATGCT+TGG 0.551076 4:-57253421 MsG0480021378.01.T01:CDS
AAGAAAGTGTACGGTGGCGA+TGG 0.556428 4:-57253440 MsG0480021378.01.T01:CDS
TTGATATTAAGAATGGTGGA+AGG 0.557402 4:-57252039 MsG0480021378.01.T01:CDS
AGATTCCGTTAGGACCAGTC+AGG 0.560990 4:+57252248 None:intergenic
GCAGAATCGCAAGAGAAACC+AGG 0.574478 4:+57251923 None:intergenic
CAATCGGCTAAGAAAGTGTA+CGG 0.575765 4:-57253449 MsG0480021378.01.T01:CDS
GATGTTGATATTAAGAATGG+TGG 0.582311 4:-57252043 MsG0480021378.01.T01:CDS
CATCAGCGGTCCTCTTTGAA+CGG 0.585723 4:+57251500 None:intergenic
CGTAACATAGGAAGCTCAGA+AGG 0.585930 4:+57253395 None:intergenic
AGAAGCTCTTCCGTTCAAAG+AGG 0.586762 4:-57251510 MsG0480021378.01.T01:CDS
ATGTTACCTTCACGTAACAT+AGG 0.589093 4:+57253383 None:intergenic
AGAGAAACCAGGAGAGCACA+TGG 0.590073 4:+57251934 None:intergenic
TTCTTAATATCAACATCCAA+TGG 0.592422 4:+57252049 None:intergenic
GATTGCTGACAATGATGGAA+TGG 0.599379 4:-57251768 MsG0480021378.01.T01:CDS
GAAGGTTGTCGCAATTAAGG+AGG 0.601090 4:-57252398 MsG0480021378.01.T01:CDS
ACTTCAAAAGCACACAAAGC+CGG 0.601124 4:-57252289 MsG0480021378.01.T01:CDS
CCTGTATTTACACACATGGC+CGG 0.609498 4:-57251604 MsG0480021378.01.T01:CDS
CAACAAAGAGGACACTGAGT+TGG 0.612479 4:-57252332 MsG0480021378.01.T01:CDS
GCTACCTGAATCAGAAGAGA+AGG 0.615858 4:-57252416 MsG0480021378.01.T01:CDS
CAACCTCACCAACCAAAGGA+AGG 0.617830 4:+57251993 None:intergenic
CAACACCAACAACCTGAACA+CGG 0.621513 4:+57251867 None:intergenic
AAAGCAGCCTGCAGAACCGT+AGG 0.627439 4:+57251553 None:intergenic
AGAGGAGCATAAGAAAGGCA+TGG 0.627567 4:-57252092 MsG0480021378.01.T01:CDS
GAGCTTCCTATGTTACGTGA+AGG 0.629318 4:-57253389 MsG0480021378.01.T01:CDS
AAACCAACCTCACCAACCAA+AGG 0.641668 4:+57251989 None:intergenic
TGGCCGGAAGTTCTTCGGTG+TGG 0.642095 4:-57251588 MsG0480021378.01.T01:CDS
ATGTTACGTGAAGGTAACAT+TGG 0.643476 4:-57253380 MsG0480021378.01.T01:CDS
AGGGAAGAAAATGGCATCAG+CGG 0.644574 4:+57251486 None:intergenic
TTGGAAGAATCAGAATACCT+CGG 0.655927 4:+57253317 None:intergenic
ATCTTCCATCAATCCTCACA+AGG 0.661742 4:+57251957 None:intergenic
AAGGTTGTCGCAATTAAGGA+GGG 0.662035 4:-57252397 MsG0480021378.01.T01:CDS
TGATATTAAGAATGGTGGAA+GGG 0.666660 4:-57252038 MsG0480021378.01.T01:CDS
GACATGGTGGTACAACAAAG+AGG 0.672878 4:-57252344 MsG0480021378.01.T01:CDS
CTGACAATGATGGAATGGAG+TGG 0.715524 4:-57251763 MsG0480021378.01.T01:CDS
TCGGCTAAGAAAGTGTACGG+TGG 0.760898 4:-57253446 MsG0480021378.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATATTGTCTATAA+CGG + Chr4:57253348-57253367 None:intergenic 10.0%
!!! ATTTTTGATTGATTTTTATA+AGG - Chr4:57251774-57251793 MsG0480021378.01.T01:CDS 10.0%
!!! TGATTATTTTGTTTAAAAAA+AGG - Chr4:57252052-57252071 MsG0480021378.01.T01:CDS 10.0%
!!! TTTTTGATTGATTTTTATAA+GGG - Chr4:57251775-57251794 MsG0480021378.01.T01:CDS 10.0%
!! AAGAAATATAACTATATCTA+AGG + Chr4:57253492-57253511 None:intergenic 15.0%
!!! AAACTTTTTATAAAGTAGAA+AGG + Chr4:57253373-57253392 None:intergenic 15.0%
!!! CATTTTTTATAACTGAATTT+TGG + Chr4:57251927-57251946 None:intergenic 15.0%
!!! TTGATTTTATTTTTGTGATA+CGG - Chr4:57252146-57252165 MsG0480021378.01.T01:CDS 15.0%
!! AAGAAATAACAAACAACAAA+AGG + Chr4:57253112-57253131 None:intergenic 20.0%
!! ATAGTTATATTTCTTCCTAT+TGG - Chr4:57253497-57253516 MsG0480021378.01.T01:five_prime_UTR 20.0%
!! ATTAACAAGAAATAACCTAA+AGG + Chr4:57253069-57253088 None:intergenic 20.0%
!! ATTTCCATTCTACTTTAATT+TGG + Chr4:57253312-57253331 None:intergenic 20.0%
!! TGATAAAAACTATAATAAGC+TGG - Chr4:57252086-57252105 MsG0480021378.01.T01:CDS 20.0%
!! TTAAAGACATATCAAAATAG+AGG + Chr4:57253455-57253474 None:intergenic 20.0%
!! TTCTACTATACTATCAATAT+TGG + Chr4:57252273-57252292 None:intergenic 20.0%
!!! AAAGAAACTGACTTTTATTT+TGG + Chr4:57251898-57251917 None:intergenic 20.0%
!!! AAAGTTTTATGTGTCTATAT+AGG - Chr4:57251728-57251747 MsG0480021378.01.T01:intron 20.0%
!!! TAAAATTTGTAAATAACGGA+TGG - Chr4:57252016-57252035 MsG0480021378.01.T01:CDS 20.0%
!!! TTGTTATTTCTTATACTTAC+TGG - Chr4:57253120-57253139 MsG0480021378.01.T01:intron 20.0%
! AAGCAAAATTTGTATCCAAT+AGG + Chr4:57253515-57253534 None:intergenic 25.0%
! AGAATTGTTGAGATTCTTAA+TGG - Chr4:57252289-57252308 MsG0480021378.01.T01:CDS 25.0%
! TAAAAAAGACGAATTAGATG+AGG + Chr4:57251511-57251530 None:intergenic 25.0%
! TGGCAAAACATAATAACATT+CGG - Chr4:57251690-57251709 MsG0480021378.01.T01:intron 25.0%
! TTCATAATTCATAGTGCTAT+TGG - Chr4:57252242-57252261 MsG0480021378.01.T01:CDS 25.0%
! TTCTTAATATCAACATCCAA+TGG + Chr4:57252723-57252742 None:intergenic 25.0%
! TTGGATGTTGATATTAAGAA+TGG - Chr4:57252723-57252742 MsG0480021378.01.T01:intron 25.0%
!! TCATAATTCATAGTGCTATT+GGG - Chr4:57252243-57252262 MsG0480021378.01.T01:CDS 25.0%
!!! AAGTTTCTGCACTTTTATTT+TGG - Chr4:57253387-57253406 MsG0480021378.01.T01:CDS 25.0%
AAGGTAAGAAAAACTCACTT+AGG + Chr4:57253050-57253069 None:intergenic 30.0%
AGGTAAGAAAAACTCACTTA+GGG + Chr4:57253049-57253068 None:intergenic 30.0%
AGGTTACTTACATTGTTAGA+GGG - Chr4:57252874-57252893 MsG0480021378.01.T01:intron 30.0%
GAAAAAATCAGTAAACTCAC+CGG + Chr4:57252502-57252521 None:intergenic 30.0%
GAAGAACATTGTTTCATACA+TGG - Chr4:57252173-57252192 MsG0480021378.01.T01:CDS 30.0%
GATGTTGATATTAAGAATGG+TGG - Chr4:57252726-57252745 MsG0480021378.01.T01:intron 30.0%
GTGTCAGATAATATAGTTGA+CGG + Chr4:57251665-57251684 None:intergenic 30.0%
TATATTATCTGACACCAACA+TGG - Chr4:57251670-57251689 MsG0480021378.01.T01:intron 30.0%
TCCTCCAAATTAAAGTAGAA+TGG - Chr4:57253305-57253324 MsG0480021378.01.T01:CDS 30.0%
! AAGAAGTTGATTGTCTCAAT+TGG - Chr4:57253416-57253435 MsG0480021378.01.T01:CDS 30.0%
! AGTGAGTTTTTCTTACCTTT+AGG - Chr4:57253051-57253070 MsG0480021378.01.T01:intron 30.0%
! GTTATTATGTTTTGCCATGT+TGG + Chr4:57251687-57251706 None:intergenic 30.0%
! TGATATTAAGAATGGTGGAA+GGG - Chr4:57252731-57252750 MsG0480021378.01.T01:intron 30.0%
! TTGATATTAAGAATGGTGGA+AGG - Chr4:57252730-57252749 MsG0480021378.01.T01:intron 30.0%
!! ATTCTACTTTAATTTGGAGG+AGG + Chr4:57253306-57253325 None:intergenic 30.0%
!! TCCATTCTACTTTAATTTGG+AGG + Chr4:57253309-57253328 None:intergenic 30.0%
!! TTCTACTTTAATTTGGAGGA+GGG + Chr4:57253305-57253324 None:intergenic 30.0%
!!! ATTGTCTCAATTGGTTTTTG+GGG - Chr4:57253425-57253444 MsG0480021378.01.T01:CDS 30.0%
!!! GATTGTCTCAATTGGTTTTT+GGG - Chr4:57253424-57253443 MsG0480021378.01.T01:CDS 30.0%
!!! TGATTGTCTCAATTGGTTTT+TGG - Chr4:57253423-57253442 MsG0480021378.01.T01:CDS 30.0%
!!! TTTACTGATTTTTTCCTGAC+TGG - Chr4:57252507-57252526 MsG0480021378.01.T01:intron 30.0%
AACAATGTGAAGACTCTTGT+TGG - Chr4:57252594-57252613 MsG0480021378.01.T01:intron 35.0%
AAGGTTGCTTATGTTCTTCA+AGG - Chr4:57251473-57251492 MsG0480021378.01.T01:exon 35.0%
AATCCAGTAAAGATTCCGTT+AGG + Chr4:57252534-57252553 None:intergenic 35.0%
ATGTTACCTTCACGTAACAT+AGG + Chr4:57251389-57251408 None:intergenic 35.0%
ATGTTACGTGAAGGTAACAT+TGG - Chr4:57251389-57251408 MsG0480021378.01.T01:three_prime_UTR 35.0%
CAGGTTACTTACATTGTTAG+AGG - Chr4:57252873-57252892 MsG0480021378.01.T01:intron 35.0%
GAACATTGTTTCATACATGG+TGG - Chr4:57252176-57252195 MsG0480021378.01.T01:CDS 35.0%
GGTAAGAAAAACTCACTTAG+GGG + Chr4:57253048-57253067 None:intergenic 35.0%
TATCGATCTTTCTCCTCAAT+CGG - Chr4:57251304-57251323 MsG0480021378.01.T01:three_prime_UTR 35.0%
TTGAAGAACATAAGCAACCT+TGG + Chr4:57251474-57251493 None:intergenic 35.0%
TTGGAAAGCAATCTGCTAAA+GGG - Chr4:57252613-57252632 MsG0480021378.01.T01:intron 35.0%
TTGGAAGAATCAGAATACCT+CGG + Chr4:57251455-57251474 None:intergenic 35.0%
! AAGTTAGCTCTTCAGAAGAA+TGG - Chr4:57251419-57251438 MsG0480021378.01.T01:three_prime_UTR 35.0%
! ATTCTTCTGAAGAGCTAACT+TGG + Chr4:57251420-57251439 None:intergenic 35.0%
! GGATTTTCAACTGAGTTTGT+GGG - Chr4:57252549-57252568 MsG0480021378.01.T01:intron 35.0%
! TGGATTTTCAACTGAGTTTG+TGG - Chr4:57252548-57252567 MsG0480021378.01.T01:intron 35.0%
! TTGTGTTGATCACTTAATCG+AGG - Chr4:57251611-57251630 MsG0480021378.01.T01:intron 35.0%
!! AGATTTGTTGATACAGGAAG+TGG - Chr4:57252315-57252334 MsG0480021378.01.T01:CDS 35.0%
!! CGATTTTTGTGTTGTTGCTA+TGG - Chr4:57251280-57251299 MsG0480021378.01.T01:three_prime_UTR 35.0%
!! TGGTGAAGATTTGTTGATAC+AGG - Chr4:57252309-57252328 MsG0480021378.01.T01:CDS 35.0%
!!! AGTTTTGGAGACAACTTTGA+AGG - Chr4:57252935-57252954 MsG0480021378.01.T01:intron 35.0%
!!! TGTTGATGGTAAGAGAGTTT+TGG - Chr4:57252920-57252939 MsG0480021378.01.T01:intron 35.0%
AAAGGGATTGTGAAGCTTGA+CGG - Chr4:57252630-57252649 MsG0480021378.01.T01:intron 40.0%
AAGAAAAACTCACTTAGGGG+TGG + Chr4:57253045-57253064 None:intergenic 40.0%
AAGGTTGTCGCAATTAAGGA+GGG - Chr4:57252372-57252391 MsG0480021378.01.T01:CDS 40.0%
ACAACCTTCTCTTCTGATTC+AGG + Chr4:57252360-57252379 None:intergenic 40.0%
ACCATGTCACAACACCAAAA+GGG + Chr4:57252413-57252432 None:intergenic 40.0%
ACGATCACACGAAAAAGCAA+AGG + Chr4:57251259-57251278 None:intergenic 40.0%
ACTTACATTGTTAGAGGGAG+CGG - Chr4:57252879-57252898 MsG0480021378.01.T01:intron 40.0%
AGAGAAGGTTGTCGCAATTA+AGG - Chr4:57252368-57252387 MsG0480021378.01.T01:CDS 40.0%
ATCTTCCATCAATCCTCACA+AGG + Chr4:57252815-57252834 None:intergenic 40.0%
ATTTGGAGGAGGGAAGAAAA+TGG + Chr4:57253295-57253314 None:intergenic 40.0%
CAGTCCTGTATTTACACACA+TGG - Chr4:57253161-57253180 MsG0480021378.01.T01:intron 40.0%
CCTTTCTTATGCTCCTCTTT+AGG + Chr4:57252675-57252694 None:intergenic 40.0%
GTTGGAAAGCAATCTGCTAA+AGG - Chr4:57252612-57252631 MsG0480021378.01.T01:intron 40.0%
TACACTTTCTTAGCCGATTG+AGG + Chr4:57251320-57251339 None:intergenic 40.0%
TCGAAGATTGCTGACAATGA+TGG - Chr4:57252996-57253015 MsG0480021378.01.T01:intron 40.0%
! ACTTCAAAAGCACACAAAGC+CGG - Chr4:57252480-57252499 MsG0480021378.01.T01:intron 40.0%
! CAATCGGCTAAGAAAGTGTA+CGG - Chr4:57251320-57251339 MsG0480021378.01.T01:three_prime_UTR 40.0%
! CCTAAAGAGGAGCATAAGAA+AGG - Chr4:57252672-57252691 MsG0480021378.01.T01:intron 40.0%
! GAGATTTGTTCATTGTGCCA+AGG - Chr4:57252961-57252980 MsG0480021378.01.T01:intron 40.0%
! GAGGTATTCTGATTCTTCCA+AGG - Chr4:57251454-57251473 MsG0480021378.01.T01:three_prime_UTR 40.0%
! GATTGCTGACAATGATGGAA+TGG - Chr4:57253001-57253020 MsG0480021378.01.T01:intron 40.0%
!! ATGGTGGATCGTATTATGCT+TGG - Chr4:57251348-57251367 MsG0480021378.01.T01:three_prime_UTR 40.0%
!! TTTTTCCTGACTGGTCCTAA+CGG - Chr4:57252516-57252535 MsG0480021378.01.T01:intron 40.0%
!!! AGAATGGTTTTGCTATTCCG+AGG - Chr4:57251435-57251454 MsG0480021378.01.T01:three_prime_UTR 40.0%
AAACCAACCTCACCAACCAA+AGG + Chr4:57252783-57252802 None:intergenic 45.0%
ACCAACCAAAGGAAGGTTCT+TGG + Chr4:57252772-57252791 None:intergenic 45.0%
ACCAAGAACCTTCCTTTGGT+TGG - Chr4:57252768-57252787 MsG0480021378.01.T01:intron 45.0%
AGAAGCTCTTCCGTTCAAAG+AGG - Chr4:57253259-57253278 MsG0480021378.01.T01:intron 45.0%
AGGGAAGAAAATGGCATCAG+CGG + Chr4:57253286-57253305 None:intergenic 45.0%
CAACAAAGAGGACACTGAGT+TGG - Chr4:57252437-57252456 MsG0480021378.01.T01:CDS 45.0%
CAACACCAACAACCTGAACA+CGG + Chr4:57252905-57252924 None:intergenic 45.0%
CACCATGTCACAACACCAAA+AGG + Chr4:57252414-57252433 None:intergenic 45.0%
CCTGTATTTACACACATGGC+CGG - Chr4:57253165-57253184 MsG0480021378.01.T01:intron 45.0%
CGTAACATAGGAAGCTCAGA+AGG + Chr4:57251377-57251396 None:intergenic 45.0%
GAACACCAAGAACCTTCCTT+TGG - Chr4:57252764-57252783 MsG0480021378.01.T01:intron 45.0%
GAAGGTTGTCGCAATTAAGG+AGG - Chr4:57252371-57252390 MsG0480021378.01.T01:CDS 45.0%
GACATGGTGGTACAACAAAG+AGG - Chr4:57252425-57252444 MsG0480021378.01.T01:CDS 45.0%
GAGCTTCCTATGTTACGTGA+AGG - Chr4:57251380-57251399 MsG0480021378.01.T01:three_prime_UTR 45.0%
GCTACCTGAATCAGAAGAGA+AGG - Chr4:57252353-57252372 MsG0480021378.01.T01:CDS 45.0%
GGTCCTAACGGAATCTTTAC+TGG - Chr4:57252528-57252547 MsG0480021378.01.T01:intron 45.0%
TGAGTTTGTGGGAAGAGCTT+GGG - Chr4:57252560-57252579 MsG0480021378.01.T01:intron 45.0%
TTATGCTCCTCTTTAGGCTG+AGG + Chr4:57252669-57252688 None:intergenic 45.0%
TTCGAGACGACGAAAAACCT+TGG + Chr4:57252981-57253000 None:intergenic 45.0%
! AGAGGAGCATAAGAAAGGCA+TGG - Chr4:57252677-57252696 MsG0480021378.01.T01:intron 45.0%
! CTGACAATGATGGAATGGAG+TGG - Chr4:57253006-57253025 MsG0480021378.01.T01:intron 45.0%
! GAGTTGGTTGTTCTGTTCCT+CGG - Chr4:57252453-57252472 MsG0480021378.01.T01:intron 45.0%
! GATGCTTTAGCACTCCCTTT+TGG - Chr4:57252396-57252415 MsG0480021378.01.T01:CDS 45.0%
! TTGGAGACAACTTTGAAGGC+TGG - Chr4:57252939-57252958 MsG0480021378.01.T01:intron 45.0%
!! AGGCATGGCTTTGAACTGTT+TGG - Chr4:57252692-57252711 MsG0480021378.01.T01:intron 45.0%
!! CATGGCTTTGAACTGTTTGG+AGG - Chr4:57252695-57252714 MsG0480021378.01.T01:intron 45.0%
!! GTTCAGGTTGTTGGTGTTGA+TGG - Chr4:57252906-57252925 MsG0480021378.01.T01:intron 45.0%
!! TTGGTTGGTGAGGTTGGTTT+AGG - Chr4:57252783-57252802 MsG0480021378.01.T01:intron 45.0%
!!! CTTTTGGTGTTGTGACATGG+TGG - Chr4:57252412-57252431 MsG0480021378.01.T01:CDS 45.0%
!!! TCCCTTTTGGTGTTGTGACA+TGG - Chr4:57252409-57252428 MsG0480021378.01.T01:CDS 45.0%
!!! TTTTTAAAATTTGTAAATAA+CGG - Chr4:57252012-57252031 MsG0480021378.01.T01:CDS 5.0%
AAGCCTTCCTCAGCCTAAAG+AGG - Chr4:57252659-57252678 MsG0480021378.01.T01:intron 50.0%
ACACATGGCCGGAAGTTCTT+CGG - Chr4:57253176-57253195 MsG0480021378.01.T01:intron 50.0%
AGATTCCGTTAGGACCAGTC+AGG + Chr4:57252524-57252543 None:intergenic 50.0%
CAACCTCACCAACCAAAGGA+AGG + Chr4:57252779-57252798 None:intergenic 50.0%
CATCAGCGGTCCTCTTTGAA+CGG + Chr4:57253272-57253291 None:intergenic 50.0%
CCGGCCATGTGTGTAAATAC+AGG + Chr4:57253168-57253187 None:intergenic 50.0%
CGGAAGAGCTTCTCAACTTC+TGG + Chr4:57253252-57253271 None:intergenic 50.0%
CTCTTGCGATTCTGCTCTAC+AGG - Chr4:57252854-57252873 MsG0480021378.01.T01:intron 50.0%
CTGAGTTTGTGGGAAGAGCT+TGG - Chr4:57252559-57252578 MsG0480021378.01.T01:intron 50.0%
CTTCCACACCGAAGAACTTC+CGG + Chr4:57253187-57253206 None:intergenic 50.0%
GATACAGGAAGTGGAGTTGC+TGG - Chr4:57252324-57252343 MsG0480021378.01.T01:CDS 50.0%
GCAGAATCGCAAGAGAAACC+AGG + Chr4:57252849-57252868 None:intergenic 50.0%
GCTGACCTTGTGAGGATTGA+TGG - Chr4:57252807-57252826 MsG0480021378.01.T01:intron 50.0%
GTTTAGGAGCTGACCTTGTG+AGG - Chr4:57252799-57252818 MsG0480021378.01.T01:intron 50.0%
! AAGAAAGTGTACGGTGGCGA+TGG - Chr4:57251329-57251348 MsG0480021378.01.T01:three_prime_UTR 50.0%
! AGAGAAACCAGGAGAGCACA+TGG + Chr4:57252838-57252857 None:intergenic 50.0%
! GAACCTTCCTTTGGTTGGTG+AGG - Chr4:57252773-57252792 MsG0480021378.01.T01:intron 50.0%
! TCGGCTAAGAAAGTGTACGG+TGG - Chr4:57251323-57251342 MsG0480021378.01.T01:three_prime_UTR 50.0%
!! CTTCCTTTGGTTGGTGAGGT+TGG - Chr4:57252777-57252796 MsG0480021378.01.T01:intron 50.0%
!! GAACTGTTTGGAGGCACCAT+TGG - Chr4:57252704-57252723 MsG0480021378.01.T01:intron 50.0%
!!! GTGCTTTTGAAGTATCGCCG+AGG + Chr4:57252473-57252492 None:intergenic 50.0%
AAAGCAGCCTGCAGAACCGT+AGG + Chr4:57253219-57253238 None:intergenic 55.0%
AAAGTGTACGGTGGCGATGG+TGG - Chr4:57251332-57251351 MsG0480021378.01.T01:three_prime_UTR 55.0%
GCTCCTCTTTAGGCTGAGGA+AGG + Chr4:57252665-57252684 None:intergenic 55.0%
GGAAGATCCATGTGCTCTCC+TGG - Chr4:57252828-57252847 MsG0480021378.01.T01:intron 55.0%
! CGGAAGTTCTTCGGTGTGGA+AGG - Chr4:57253185-57253204 MsG0480021378.01.T01:intron 55.0%
GCTATCGCCTACGGTTCTGC+AGG - Chr4:57253209-57253228 MsG0480021378.01.T01:intron 60.0%
TGGCCGGAAGTTCTTCGGTG+TGG - Chr4:57253181-57253200 MsG0480021378.01.T01:intron 60.0%
!! AGCGGCCGTGTTCAGGTTGT+TGG - Chr4:57252897-57252916 MsG0480021378.01.T01:intron 60.0%
!! GTGGAAGGCGCTATCGCCTA+CGG - Chr4:57253200-57253219 MsG0480021378.01.T01:intron 60.0%
TAGAGGGAGCGGCCGTGTTC+AGG - Chr4:57252890-57252909 MsG0480021378.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 57251226 57253565 57251226 ID=MsG0480021378.01;Name=MsG0480021378.01
Chr4 mRNA 57251226 57253565 57251226 ID=MsG0480021378.01.T01;Parent=MsG0480021378.01;Name=MsG0480021378.01.T01;_AED=0.47;_eAED=0.47;_QI=73|1|1|1|1|1|3|248|356
Chr4 exon 57251226 57251627 57251226 ID=MsG0480021378.01.T01:exon:7227;Parent=MsG0480021378.01.T01
Chr4 exon 57251740 57252460 57251740 ID=MsG0480021378.01.T01:exon:7226;Parent=MsG0480021378.01.T01
Chr4 exon 57253297 57253565 57253297 ID=MsG0480021378.01.T01:exon:7225;Parent=MsG0480021378.01.T01
Chr4 five_prime_UTR 57253493 57253565 57253493 ID=MsG0480021378.01.T01:five_prime_utr;Parent=MsG0480021378.01.T01
Chr4 CDS 57253297 57253492 57253297 ID=MsG0480021378.01.T01:cds;Parent=MsG0480021378.01.T01
Chr4 CDS 57251740 57252460 57251740 ID=MsG0480021378.01.T01:cds;Parent=MsG0480021378.01.T01
Chr4 CDS 57251474 57251627 57251474 ID=MsG0480021378.01.T01:cds;Parent=MsG0480021378.01.T01
Chr4 three_prime_UTR 57251226 57251473 57251226 ID=MsG0480021378.01.T01:three_prime_utr;Parent=MsG0480021378.01.T01
Gene Sequence

>MsG0480021378.01.T01

ATGGATATCGATCTTTCTCCTCAATCGGCTAAGAAAGTGTACGGTGGCGATGGTGGATCGTATTATGCTTGGTCTCCTTCTGAGCTTCCTATGTTACGTGAAGGTAACATTGGTGCTGCCAAGTTAGCTCTTCAGAAGAATGGTTTTGCTATTCCGAGGTATTCTGATTCTTCCAAGGTTGCTTATGTTCTTCAAGGAAGTGGAGTTGCTGGAATTGTGCTACCTGAATCAGAAGAGAAGGTTGTCGCAATTAAGGAGGGTGATGCTTTAGCACTCCCTTTTGGTGTTGTGACATGGTGGTACAACAAAGAGGACACTGAGTTGGTTGTTCTGTTCCTCGGCGATACTTCAAAAGCACACAAAGCCGGTGAGTTTACTGATTTTTTCCTGACTGGTCCTAACGGAATCTTTACTGGATTTTCAACTGAGTTTGTGGGAAGAGCTTGGGACTTAGACGAGAACAATGTGAAGACTCTTGTTGGAAAGCAATCTGCTAAAGGGATTGTGAAGCTTGACGGAAAAATAAGCCTTCCTCAGCCTAAAGAGGAGCATAAGAAAGGCATGGCTTTGAACTGTTTGGAGGCACCATTGGATGTTGATATTAAGAATGGTGGAAGGGTTGTTGTCTTGAACACCAAGAACCTTCCTTTGGTTGGTGAGGTTGGTTTAGGAGCTGACCTTGTGAGGATTGATGGAAGATCCATGTGCTCTCCTGGTTTCTCTTGCGATTCTGCTCTACAGGTTACTTACATTGTTAGAGGGAGCGGCCGTGTTCAGGTTGTTGGTGTTGATGGTAAGAGAGTTTTGGAGACAACTTTGAAGGCTGGAGATTTGTTCATTGTGCCAAGGTTTTTCGTCGTCTCGAAGATTGCTGACAATGATGGAATGGAGTGGTTTTCTATCATCACCACCCCTAATCCTGTATTTACACACATGGCCGGAAGTTCTTCGGTGTGGAAGGCGCTATCGCCTACGGTTCTGCAGGCTGCTTTCAATGTAGATCCAGAAGTTGAGAAGCTCTTCCGTTCAAAGAGGACCGCTGATGCCATTTTCTTCCCTCCTCCAAATTAA
Protein sequence

>MsG0480021378.01.T01

MDIDLSPQSAKKVYGGDGGSYYAWSPSELPMLREGNIGAAKLALQKNGFAIPRYSDSSKVAYVLQGSGVAGIVLPESEEKVVAIKEGDALALPFGVVTWWYNKEDTELVVLFLGDTSKAHKAGEFTDFFLTGPNGIFTGFSTEFVGRAWDLDENNVKTLVGKQSAKGIVKLDGKISLPQPKEEHKKGMALNCLEAPLDVDIKNGGRVVVLNTKNLPLVGEVGLGADLVRIDGRSMCSPGFSCDSALQVTYIVRGSGRVQVVGVDGKRVLETTLKAGDLFIVPRFFVVSKIADNDGMEWFSIITTPNPVFTHMAGSSSVWKALSPTVLQAAFNVDPEVEKLFRSKRTADAIFFPPPN*