AlfalfaGEDB | MsG0480021380.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021380.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021380.01.T01	AES89350.1	91.86	86	7	0	1	86	1	86	1.28E-50	164

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021380.01.T01	P33081	76.471	85	17	2	1	85	1	82	1.08E-39	129

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021380.01.T01	G7JMA7	91.860	86	7	0	1	86	1	86	6.11e-51	164

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380013499.01	MsG0480021380.01	0.805852	1.092640e-49	5.842890e-47
MsG0380014386.01	MsG0480021380.01	0.801589	8.385481e-49	4.019721e-46
MsG0480021380.01	MsG0480023661.01	0.804947	1.691229e-49	8.835884e-47
MsG0480021380.01	MsG0580024759.01	0.816186	6.296170e-52	4.430774e-49
MsG0480021380.01	MsG0780039975.01	0.807434	5.063714e-50	2.820108e-47
MsG0480021380.01	MsG0880043249.01	0.800441	1.439027e-48	6.701074e-46
MsG0380014926.01	MsG0480021380.01	0.812904	3.351952e-51	2.157575e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021380.01.T01	MTR_4g072820	91.860	86	7	0	1	86	1	86	1.55e-54	164
MsG0480021380.01.T01	MTR_4g072610	77.381	84	18	1	1	84	1	83	3.56e-43	135
MsG0480021380.01.T01	MTR_3g084150	71.429	91	19	2	1	85	1	90	1.04e-42	134
MsG0480021380.01.T01	MTR_4g072580	78.824	85	16	2	1	84	1	84	1.51e-42	133
MsG0480021380.01.T01	MTR_4g072740	76.744	86	18	2	1	85	1	85	1.87e-42	133
MsG0480021380.01.T01	MTR_4g072370	74.157	89	18	2	1	85	1	88	3.97e-42	132
MsG0480021380.01.T01	MTR_4g072650	77.381	84	18	1	1	84	1	83	4.07e-42	132
MsG0480021380.01.T01	MTR_3g084180	70.330	91	20	2	1	85	1	90	9.07e-42	132
MsG0480021380.01.T01	MTR_4g072490	75.581	86	19	2	1	85	1	85	1.04e-41	131
MsG0480021380.01.T01	MTR_4g072380	78.313	83	17	1	1	82	1	83	3.41e-41	130
MsG0480021380.01.T01	MTR_8g461410	68.132	91	22	2	1	85	1	90	3.42e-41	130
MsG0480021380.01.T01	MTR_4g072310	69.565	92	20	2	1	85	1	91	6.10e-40	127
MsG0480021380.01.T01	MTR_4g072870	68.132	91	23	2	1	85	1	91	6.35e-40	127
MsG0480021380.01.T01	MTR_4g072470	74.419	86	20	2	1	85	1	85	6.71e-40	127
MsG0480021380.01.T01	MTR_3g084240	70.000	90	22	2	1	85	1	90	1.06e-39	126
MsG0480021380.01.T01	MTR_3g110038	68.889	90	23	2	1	85	1	90	1.16e-39	126
MsG0480021380.01.T01	MTR_4g072500	68.889	90	23	1	1	85	1	90	1.61e-39	126
MsG0480021380.01.T01	MTR_3g084170	68.889	90	23	2	1	85	1	90	4.09e-39	125
MsG0480021380.01.T01	MTR_3g110042	70.000	90	20	2	1	84	1	89	5.09e-39	125
MsG0480021380.01.T01	MTR_4g072260	68.889	90	22	2	1	84	1	90	5.18e-39	125
MsG0480021380.01.T01	MTR_4g072330	65.909	88	27	1	1	85	1	88	6.52e-39	124
MsG0480021380.01.T01	MTR_4g072240	64.835	91	26	1	1	85	1	91	6.79e-39	124
MsG0480021380.01.T01	MTR_4g072220	68.889	90	22	2	1	84	1	90	6.89e-39	124
MsG0480021380.01.T01	MTR_4g072630	72.414	87	21	2	1	85	1	86	1.12e-38	124
MsG0480021380.01.T01	MTR_4g072640	67.778	90	24	2	1	85	1	90	3.91e-38	122
MsG0480021380.01.T01	MTR_4g072530	66.667	90	25	1	1	85	1	90	3.99e-38	122
MsG0480021380.01.T01	MTR_4g050700	67.033	91	24	2	1	85	1	91	6.97e-38	122
MsG0480021380.01.T01	MTR_2g012410	67.033	91	24	2	1	85	1	91	6.97e-38	122
MsG0480021380.01.T01	MTR_4g072830	72.727	88	19	3	1	84	1	87	9.69e-38	121
MsG0480021380.01.T01	MTR_4g072670	74.713	87	19	2	1	85	1	86	1.01e-37	121
MsG0480021380.01.T01	MTR_4g072300	62.637	91	28	1	1	85	1	91	1.30e-37	121
MsG0480021380.01.T01	MTR_4g072890	65.934	91	25	2	1	85	1	91	1.43e-37	121
MsG0480021380.01.T01	MTR_3g084250	68.132	91	22	2	1	85	1	90	1.44e-37	121
MsG0480021380.01.T01	MTR_4g072465	71.264	87	22	2	1	85	1	86	1.46e-37	121
MsG0480021380.01.T01	MTR_4g072880	67.391	92	22	3	1	85	1	91	1.50e-37	121
MsG0480021380.01.T01	MTR_4g072600	72.941	85	21	2	1	84	1	84	1.73e-37	120
MsG0480021380.01.T01	MTR_4g072540	74.713	87	19	2	1	85	1	86	4.01e-37	120
MsG0480021380.01.T01	MTR_3g084220	65.556	90	26	2	1	85	1	90	5.69e-37	119
MsG0480021380.01.T01	MTR_4g072850	65.591	93	23	2	1	85	1	92	6.16e-37	119
MsG0480021380.01.T01	MTR_4g072720	73.563	87	20	2	1	85	1	86	6.28e-37	119
MsG0480021380.01.T01	MTR_4g072230	70.000	90	17	3	1	83	1	87	1.30e-36	119
MsG0480021380.01.T01	MTR_4g072480	64.835	91	25	2	1	85	1	90	1.74e-36	118
MsG0480021380.01.T01	MTR_3g084260	62.637	91	28	1	1	85	1	91	1.75e-36	118
MsG0480021380.01.T01	MTR_4g072590	71.765	85	24	0	1	85	1	85	1.95e-36	118
MsG0480021380.01.T01	MTR_3g117630	67.442	86	21	2	6	85	6	90	3.01e-36	118
MsG0480021380.01.T01	MTR_4g072250	68.478	92	20	3	1	85	1	90	4.37e-36	117
MsG0480021380.01.T01	MTR_4g072570	64.835	91	25	2	1	85	1	90	5.44e-36	117
MsG0480021380.01.T01	MTR_8g461420	70.000	90	22	1	1	85	1	90	5.69e-36	117
MsG0480021380.01.T01	MTR_4g072510	71.264	87	22	2	1	85	1	86	1.58e-35	115
MsG0480021380.01.T01	MTR_3g084190	67.778	90	24	1	1	85	1	90	2.39e-35	115
MsG0480021380.01.T01	MTR_3g110032	64.130	92	25	3	1	85	1	91	2.44e-35	115
MsG0480021380.01.T01	MTR_4g072620	61.538	91	28	2	1	85	1	90	3.71e-35	115
MsG0480021380.01.T01	MTR_3g084230	69.231	91	21	2	1	85	1	90	4.05e-35	115
MsG0480021380.01.T01	MTR_4g072660	64.634	82	29	0	1	82	1	82	5.55e-35	114
MsG0480021380.01.T01	MTR_4g072680	62.637	91	27	2	1	85	1	90	6.14e-35	114
MsG0480021380.01.T01	MTR_4g072730	61.538	91	28	2	1	85	1	90	7.73e-35	114
MsG0480021380.01.T01	MTR_3g084210	67.033	91	23	2	1	85	1	90	8.17e-35	114
MsG0480021380.01.T01	MTR_4g072690	68.235	85	27	0	1	85	1	85	1.29e-34	113
MsG0480021380.01.T01	MTR_4g072270	64.045	89	24	2	1	82	1	88	1.36e-34	114
MsG0480021380.01.T01	MTR_4g072770	64.706	85	25	1	1	85	1	80	1.42e-34	113
MsG0480021380.01.T01	MTR_5g008350	74.667	75	17	2	1	74	1	74	3.51e-34	112
MsG0480021380.01.T01	MTR_3g110045	69.663	89	20	2	3	85	62	149	7.62e-34	114
MsG0480021380.01.T01	MTR_4g072860	64.130	92	25	2	1	85	1	91	1.03e-33	111
MsG0480021380.01.T01	MTR_4g072460	60.440	91	29	2	1	85	1	90	1.68e-33	111
MsG0480021380.01.T01	MTR_3g084160	65.934	91	24	2	1	85	1	90	5.81e-33	109
MsG0480021380.01.T01	MTR_4g072940	63.736	91	24	2	3	85	33	122	1.18e-32	110
MsG0480021380.01.T01	MTR_4g072450	65.882	85	29	0	1	85	1	85	1.55e-32	108
MsG0480021380.01.T01	MTR_5g008370	78.788	66	14	0	20	85	25	90	1.56e-32	110
MsG0480021380.01.T01	MTR_5g008360	67.105	76	24	1	10	85	7	81	8.67e-32	106
MsG0480021380.01.T01	MTR_4g072980	55.172	87	38	1	1	86	8	94	1.02e-30	104
MsG0480021380.01.T01	MTR_4g072550	61.446	83	28	1	7	85	19	101	2.08e-30	103
MsG0480021380.01.T01	MTR_4g072560	59.524	84	31	2	4	86	18	99	6.69e-30	102
MsG0480021380.01.T01	MTR_4g072840	62.791	86	7	2	1	86	1	61	1.30e-28	97.8
MsG0480021380.01.T01	MTR_4g072910	61.538	78	27	1	8	85	26	100	1.83e-28	98.6
MsG0480021380.01.T01	MTR_3g084200	68.493	73	16	3	1	67	1	72	2.22e-28	98.6
MsG0480021380.01.T01	MTR_3g117640	65.672	67	22	1	20	85	34	100	4.52e-28	97.8
MsG0480021380.01.T01	MTR_4g072290	57.647	85	28	1	1	85	1	77	2.76e-26	92.4
MsG0480021380.01.T01	MTR_4g072750	65.753	73	18	1	1	66	1	73	3.18e-26	92.4
MsG0480021380.01.T01	MTR_3g110035	64.062	64	23	0	22	85	73	136	4.44e-24	88.6
MsG0480021380.01.T01	MTR_3g113310	57.576	66	27	1	20	85	42	106	1.53e-23	86.3
MsG0480021380.01.T01	MTR_4g072930	52.500	80	34	1	10	85	31	110	8.98e-23	84.7
MsG0480021380.01.T01	MTR_4g072280	54.667	75	28	1	1	69	1	75	7.03e-22	81.3
MsG0480021380.01.T01	MTR_3g117610	60.317	63	24	1	20	82	45	106	9.44e-22	82.0
MsG0480021380.01.T01	MTR_6g077850	56.923	65	28	0	20	84	34	98	2.55e-21	80.5
MsG0480021380.01.T01	MTR_4g072190	56.061	66	28	1	20	85	36	100	6.98e-21	79.3
MsG0480021380.01.T01	MTR_8g076040	55.385	65	28	1	18	82	39	102	2.56e-19	75.5
MsG0480021380.01.T01	MTR_5g021820	47.500	80	40	2	7	85	26	104	3.46e-19	75.1
MsG0480021380.01.T01	MTR_4g124750	48.649	74	37	1	12	85	61	133	4.70e-17	71.2
MsG0480021380.01.T01	MTR_2g043960	52.239	67	28	2	20	86	76	138	2.51e-16	69.3
MsG0480021380.01.T01	MTR_8g461400	44.444	90	19	3	1	85	1	64	2.66e-16	67.0
MsG0480021380.01.T01	MTR_1g009150	50.746	67	32	1	11	77	62	127	4.07e-16	69.3
MsG0480021380.01.T01	MTR_4g005310	47.059	68	35	1	14	80	56	123	4.27e-16	68.6
MsG0480021380.01.T01	MTR_4g005320	50.820	61	29	1	17	77	67	126	7.16e-15	65.9
MsG0480021380.01.T01	MTR_1g094750	49.180	61	30	1	20	80	50	109	9.15e-15	64.3
MsG0480021380.01.T01	MTR_1g024085	47.826	69	35	1	9	77	26	93	1.42e-14	64.7
MsG0480021380.01.T01	MTR_4g025830	44.118	68	34	2	20	84	79	145	3.15e-14	63.9
MsG0480021380.01.T01	MTR_3g109160	51.667	60	28	1	18	77	67	125	1.52e-13	62.4
MsG0480021380.01.T01	MTR_7g118010	40.741	81	40	3	2	80	10	84	5.97e-13	59.3
MsG0480021380.01.T01	MTR_7g101740	47.692	65	33	1	18	82	38	101	6.24e-13	59.7
MsG0480021380.01.T01	MTR_8g461210	46.667	60	31	1	18	77	33	91	2.21e-12	58.2
MsG0480021380.01.T01	MTR_1g063950	45.902	61	32	1	20	80	48	107	2.83e-12	58.2
MsG0480021380.01.T01	MTR_4g072160	48.387	62	29	2	20	80	37	96	3.38e-12	58.2
MsG0480021380.01.T01	MTR_7g104950	33.766	77	50	1	10	86	40	115	4.38e-12	57.8
MsG0480021380.01.T01	MTR_3g092220	50.000	60	27	2	20	77	33	91	5.59e-12	57.0
MsG0480021380.01.T01	MTR_8g026730	44.262	61	31	2	19	77	26	85	7.00e-12	56.6
MsG0480021380.01.T01	MTR_1g059770	35.211	71	45	1	16	86	45	114	8.73e-12	57.0
MsG0480021380.01.T01	MTR_3g105900	71.429	35	10	0	20	54	1172	1206	9.55e-12	59.3
MsG0480021380.01.T01	MTR_1g061570	46.970	66	34	1	12	77	11	75	9.66e-12	56.6
MsG0480021380.01.T01	MTR_2g066830	39.706	68	36	2	5	72	47	109	1.58e-11	56.6
MsG0480021380.01.T01	MTR_2g095430	40.845	71	37	2	7	73	32	101	1.73e-11	56.2
MsG0480021380.01.T01	MTR_7g405740	37.838	74	45	1	7	80	54	126	2.20e-11	56.6
MsG0480021380.01.T01	MTR_4g021810	47.761	67	33	2	12	77	18	83	2.37e-11	55.1
MsG0480021380.01.T01	MTR_8g096470	43.333	60	33	1	21	80	47	105	4.90e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021380.01.T01	AT5G18050	64.474	76	26	1	11	85	15	90	3.74e-32	107
MsG0480021380.01.T01	AT5G18020	64.474	76	26	1	11	85	15	90	4.39e-32	107
MsG0480021380.01.T01	AT5G18060	64.474	76	26	1	11	85	15	90	1.32e-31	106
MsG0480021380.01.T01	AT4G38840	71.642	67	18	1	20	85	33	99	1.33e-31	106
MsG0480021380.01.T01	AT5G18080	64.474	76	26	1	11	85	15	90	1.38e-31	106
MsG0480021380.01.T01	AT5G18030	58.537	82	33	1	5	85	7	88	1.91e-30	103
MsG0480021380.01.T01	AT5G18010	61.842	76	28	1	11	85	15	90	1.79e-29	100
MsG0480021380.01.T01	AT2G21200	62.338	77	27	2	9	84	11	86	2.59e-29	100
MsG0480021380.01.T01	AT2G21210	55.789	95	30	3	1	84	1	94	2.80e-29	100
MsG0480021380.01.T01	AT4G34810	60.976	82	27	2	9	85	37	118	6.27e-29	100
MsG0480021380.01.T01	AT3G03840	55.172	87	31	2	1	85	14	94	8.02e-29	99.4
MsG0480021380.01.T01	AT2G21210	55.789	95	30	3	1	84	68	161	1.24e-28	100
MsG0480021380.01.T01	AT4G38850	59.259	81	25	2	8	85	14	89	1.51e-28	98.6
MsG0480021380.01.T01	AT4G38825	61.765	68	25	1	19	85	22	89	4.97e-28	97.4
MsG0480021380.01.T01	AT4G34800	55.556	90	36	2	1	86	1	90	6.25e-28	97.1
MsG0480021380.01.T01	AT3G03820	53.409	88	32	2	1	85	14	95	8.32e-28	97.1
MsG0480021380.01.T01	AT4G34770	62.162	74	26	1	13	84	28	101	2.12e-27	96.3
MsG0480021380.01.T01	AT3G03850	55.294	85	36	1	3	85	8	92	1.22e-26	94.0
MsG0480021380.01.T01	AT4G13790	50.000	80	37	2	8	85	14	92	7.52e-25	89.4
MsG0480021380.01.T01	AT3G03830	60.294	68	24	1	21	85	25	92	1.31e-24	88.6
MsG0480021380.01.T01	AT4G34790	60.294	68	25	1	20	85	39	106	4.34e-23	85.5
MsG0480021380.01.T01	AT4G38860	57.576	66	28	0	20	85	40	105	1.53e-22	84.0
MsG0480021380.01.T01	AT4G34780	50.000	80	37	1	9	85	17	96	5.72e-22	82.4
MsG0480021380.01.T01	AT1G75580	59.091	66	26	1	20	85	44	108	2.78e-21	80.9
MsG0480021380.01.T01	AT2G21220	55.385	65	28	1	20	84	40	103	2.05e-20	78.6
MsG0480021380.01.T01	AT4G34760	54.545	66	29	1	20	85	43	107	3.19e-20	78.2
MsG0480021380.01.T01	AT2G16580	54.545	66	29	1	20	85	44	108	9.42e-20	77.0
MsG0480021380.01.T01	AT1G19830	59.701	67	25	2	20	85	48	113	5.28e-19	75.1
MsG0480021380.01.T01	AT4G36110	53.846	65	29	1	18	82	38	101	2.44e-18	73.2
MsG0480021380.01.T01	AT2G18010	55.556	63	27	1	20	82	48	109	7.97e-18	72.0
MsG0480021380.01.T01	AT3G43120	52.239	67	28	2	20	86	79	141	1.37e-17	72.8
MsG0480021380.01.T01	AT5G66260	46.753	77	40	1	9	85	23	98	2.57e-16	67.8
MsG0480021380.01.T01	AT5G20810	52.239	67	28	2	20	86	79	141	6.17e-16	68.6
MsG0480021380.01.T01	AT3G51200	49.254	67	33	1	14	80	34	99	8.32e-16	66.6
MsG0480021380.01.T01	AT5G20810	52.239	67	28	2	20	86	79	141	9.96e-16	68.6
MsG0480021380.01.T01	AT3G20220	43.662	71	38	2	11	80	38	107	2.62e-15	65.9
MsG0480021380.01.T01	AT3G60690	43.662	71	36	2	18	85	86	155	2.17e-14	64.7
MsG0480021380.01.T01	AT2G45210	42.466	73	38	2	15	84	74	145	2.81e-14	64.3
MsG0480021380.01.T01	AT5G10990	41.573	89	41	3	4	86	29	112	6.24e-14	63.2
MsG0480021380.01.T01	AT2G37030	49.180	61	30	1	20	80	49	108	1.15e-13	61.6
MsG0480021380.01.T01	AT4G31320	47.143	70	36	1	8	77	68	136	1.52e-13	62.8
MsG0480021380.01.T01	AT2G24400	47.143	70	36	1	8	77	52	120	1.53e-13	62.8
MsG0480021380.01.T01	AT1G75590	47.059	68	34	2	11	77	32	98	1.26e-12	59.7
MsG0480021380.01.T01	AT4G34750	43.284	67	37	1	11	77	28	93	1.37e-12	59.7
MsG0480021380.01.T01	AT4G34750	43.284	67	37	1	11	77	28	93	1.37e-12	59.7
MsG0480021380.01.T01	AT3G61900	40.845	71	38	2	10	77	20	89	2.22e-12	58.5
MsG0480021380.01.T01	AT1G19840	47.143	70	35	2	9	77	29	97	7.36e-12	57.8
MsG0480021380.01.T01	AT2G46690	41.096	73	38	3	8	77	13	83	1.14e-11	56.6
MsG0480021380.01.T01	AT1G56150	41.270	63	36	1	10	72	28	89	1.33e-11	56.2
MsG0480021380.01.T01	AT3G12830	41.429	70	38	2	3	72	35	101	1.49e-11	56.6
MsG0480021380.01.T01	AT4G09530	41.429	70	38	2	14	80	22	91	1.72e-11	55.5
MsG0480021380.01.T01	AT4G12410	39.189	74	41	2	14	84	70	142	2.86e-11	56.2
MsG0480021380.01.T01	AT3G53250	42.623	61	31	1	20	80	52	108	3.17e-11	55.5
MsG0480021380.01.T01	AT4G00880	48.387	62	29	2	18	77	26	86	4.20e-11	55.1

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGCAGAAGAAGAATT+TGG	0.279041	4:-57270959	MsG0480021380.01.T01:CDS
CTTTCTTACAACTCTCTTCT+CGG	0.306926	4:-57270889	MsG0480021380.01.T01:CDS
TTCGCTTATCTGGTATTAGA+AGG	0.346910	4:-57271108	MsG0480021380.01.T01:CDS
TAAACTGCGAGGTAGCCTTT+TGG	0.378136	4:+57271055	None:intergenic
AAGCTGGTTCAATATTGAAA+TGG	0.380921	4:+57271000	None:intergenic
AGCTGGTTCAATATTGAAAT+GGG	0.445913	4:+57271001	None:intergenic
ATTTGGATATGATCATCCTA+TGG	0.456415	4:-57270942	MsG0480021380.01.T01:CDS
GTAATTCTTGAAATGAAAGC+TGG	0.496692	4:+57270984	None:intergenic
TAAGAAAGCATCTTCACTGC+AGG	0.497337	4:+57270904	None:intergenic
TGGGATTGCATCCACAGCCT+TGG	0.507805	4:+57271075	None:intergenic
GTCATCATCTTCCAAGGCTG+TGG	0.512173	4:-57271086	MsG0480021380.01.T01:CDS
GGCTACCTCGCAGTTTATGT+TGG	0.513695	4:-57271049	MsG0480021380.01.T01:CDS
GCTGTGGATGCAATCCCAAA+AGG	0.559098	4:-57271070	MsG0480021380.01.T01:CDS
TTTGGATATGATCATCCTAT+GGG	0.586077	4:-57270941	MsG0480021380.01.T01:CDS
TAGAAGGTCATCATCTTCCA+AGG	0.592917	4:-57271092	MsG0480021380.01.T01:CDS
AAGAAAGCATCTTCACTGCA+GGG	0.633693	4:+57270905	None:intergenic
GGATATGATCATCCTATGGG+TGG	0.645721	4:-57270938	MsG0480021380.01.T01:CDS
GGAATTGTGAGACCACCCAT+AGG	0.657747	4:+57270926	None:intergenic
TTTCTCCAACATAAACTGCG+AGG	0.736972	4:+57271044	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTTGGATATGATCATCCTA+TGG	-	Chr4:57271050-57271069	MsG0480021380.01.T01:CDS	30.0%
	GTAATTCTTGAAATGAAAGC+TGG	+	Chr4:57271011-57271030	None:intergenic	30.0%
	TTTGGATATGATCATCCTAT+GGG	-	Chr4:57271051-57271070	MsG0480021380.01.T01:CDS	30.0%
!	AAGCTGGTTCAATATTGAAA+TGG	+	Chr4:57270995-57271014	None:intergenic	30.0%
!	AGCTGGTTCAATATTGAAAT+GGG	+	Chr4:57270994-57271013	None:intergenic	30.0%
	CATCAAGCAGAAGAAGAATT+TGG	-	Chr4:57271033-57271052	MsG0480021380.01.T01:CDS	35.0%
	CTTTCTTACAACTCTCTTCT+CGG	-	Chr4:57271103-57271122	MsG0480021380.01.T01:CDS	35.0%
	TTCGCTTATCTGGTATTAGA+AGG	-	Chr4:57270884-57270903	MsG0480021380.01.T01:CDS	35.0%
	TAGAAGGTCATCATCTTCCA+AGG	-	Chr4:57270900-57270919	MsG0480021380.01.T01:CDS	40.0%
	TTTCTCCAACATAAACTGCG+AGG	+	Chr4:57270951-57270970	None:intergenic	40.0%
!!	AAGAAAGCATCTTCACTGCA+GGG	+	Chr4:57271090-57271109	None:intergenic	40.0%
!!	TAAGAAAGCATCTTCACTGC+AGG	+	Chr4:57271091-57271110	None:intergenic	40.0%
	GGATATGATCATCCTATGGG+TGG	-	Chr4:57271054-57271073	MsG0480021380.01.T01:CDS	45.0%
!	AAACTGCGAGGTAGCCTTTT+GGG	+	Chr4:57270939-57270958	None:intergenic	45.0%
!	TAAACTGCGAGGTAGCCTTT+TGG	+	Chr4:57270940-57270959	None:intergenic	45.0%
	GCTGTGGATGCAATCCCAAA+AGG	-	Chr4:57270922-57270941	MsG0480021380.01.T01:CDS	50.0%
	GGAATTGTGAGACCACCCAT+AGG	+	Chr4:57271069-57271088	None:intergenic	50.0%
	GGCTACCTCGCAGTTTATGT+TGG	-	Chr4:57270943-57270962	MsG0480021380.01.T01:CDS	50.0%
	GTCATCATCTTCCAAGGCTG+TGG	-	Chr4:57270906-57270925	MsG0480021380.01.T01:CDS	50.0%
	TGGGATTGCATCCACAGCCT+TGG	+	Chr4:57270920-57270939	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	57270877	57271137	57270877	ID=MsG0480021380.01;Name=MsG0480021380.01
Chr4	mRNA	57270877	57271137	57270877	ID=MsG0480021380.01.T01;Parent=MsG0480021380.01;Name=MsG0480021380.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|86
Chr4	exon	57270877	57271137	57270877	ID=MsG0480021380.01.T01:exon:7231;Parent=MsG0480021380.01.T01
Chr4	CDS	57270877	57271137	57270877	ID=MsG0480021380.01.T01:cds;Parent=MsG0480021380.01.T01

Gene Sequence	>MsG0480021380.01.T01 ATGGGTTTTCGCTTATCTGGTATTAGAAGGTCATCATCTTCCAAGGCTGTGGATGCAATCCCAAAAGGCTACCTCGCAGTTTATGTTGGAGAAAAAATGAAGCGATTTGTGATTCCCATTTCAATATTGAACCAGCTTTCATTTCAAGAATTACTACATCAAGCAGAAGAAGAATTTGGATATGATCATCCTATGGGTGGTCTCACAATTCCCTGCAGTGAAGATGCTTTCTTACAACTCTCTTCTCGGCTTCAGTGTTAA
Protein sequence	>MsG0480021380.01.T01 MGFRLSGIRRSSSSKAVDAIPKGYLAVYVGEKMKRFVIPISILNQLSFQELLHQAEEEFGYDHPMGGLTIPCSEDAFLQLSSRLQC*