AlfalfaGEDB | MsG0480023661.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023661.01

KEGG: -
GO: -
PFAM: PF03417

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023661.01.T01	XP_013447146.1	96.861	223	4	1	6	225	1	223	8.58E-153	439

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023661.01.T01	A0A072TWB5	96.861	223	4	1	6	225	1	223	4.10e-153	439

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047899.01	MsG0480023661.01	0.816566	5.176787e-52	3.681015e-49
MsG0080047945.01	MsG0480023661.01	0.815814	7.623141e-52	5.310206e-49
MsG0080047955.01	MsG0480023661.01	0.887829	1.011259e-72	7.639786e-69
MsG0080047956.01	MsG0480023661.01	0.860893	1.502858e-63	4.172575e-60
MsG0080047989.01	MsG0480023661.01	0.835869	1.305138e-56	1.616761e-53
MsG0080048068.01	MsG0480023661.01	0.819418	1.173783e-52	9.027501e-50
MsG0080048528.01	MsG0480023661.01	0.827126	1.858321e-54	1.777692e-51
MsG0080048727.01	MsG0480023661.01	0.857197	1.923704e-62	4.709554e-59
MsG0080048773.01	MsG0480023661.01	0.855125	7.781753e-62	1.777781e-58
MsG0080048777.01	MsG0480023661.01	0.876326	1.526283e-68	7.368492e-65

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023661.01.T01	MTR_8g099160	96.861	223	4	1	6	225	1	223	1.04e-156	439

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 54 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAAATTCCCTATGTAT+TGG	0.366578	4:-88234023	MsG0480023661.01.T01:CDS
AAACTTGAGATGTTTGAAGT+TGG	0.476023	4:-88234210	MsG0480023661.01.T01:CDS
AAATTGTAGCTGGTGGGATT+GGG	0.464490	4:-88233373	MsG0480023661.01.T01:CDS
AACCAAATTCCCTATGTATT+GGG	0.374000	4:-88234022	MsG0480023661.01.T01:CDS
AACTGTAGAACACTCATTTG+AGG	0.494365	4:+88233786	None:intergenic
AAGAGTTCATCCCAATACAT+AGG	0.525942	4:+88234012	None:intergenic
AAGATATTTACATCAAGAAG+AGG	0.587862	4:+88233964	None:intergenic
AAGATGAAATTGTAGCTGGT+GGG	0.668109	4:-88233379	MsG0480023661.01.T01:CDS
ACAATTTCATCTTCAGCTGG+TGG	0.624043	4:+88233389	None:intergenic
ACACACACAGGAACGTGAAA+TGG	0.362028	4:-88234256	MsG0480023661.01.T01:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACCAAATTCCCTATGTAT+TGG	-	Chr4:88233563-88233582	MsG0480023661.01.T01:CDS	30.0%
AACCAAATTCCCTATGTATT+GGG	-	Chr4:88233564-88233583	MsG0480023661.01.T01:CDS	30.0%
ATGCATAGATTCATACGAAA+TGG	-	Chr4:88233402-88233421	MsG0480023661.01.T01:CDS	30.0%
ATTTCGTATGAATCTATGCA+TGG	+	Chr4:88233403-88233422	None:intergenic	30.0%
CATAACAGAAAGACCAAAAA+AGG	+	Chr4:88233960-88233979	None:intergenic	30.0%
GCATTTGCATTTAATAGCAA+TGG	-	Chr4:88234059-88234078	MsG0480023661.01.T01:CDS	30.0%
TGCATAGATTCATACGAAAT+GGG	-	Chr4:88233403-88233422	MsG0480023661.01.T01:CDS	30.0%
TGCATTTAATAGCAATGGAT+TGG	-	Chr4:88234064-88234083	MsG0480023661.01.T01:CDS	30.0%
AACTGTAGAACACTCATTTG+AGG	+	Chr4:88233803-88233822	None:intergenic	35.0%
AAGAGTTCATCCCAATACAT+AGG	+	Chr4:88233577-88233596	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	88233310	88234298	88233310	ID=MsG0480023661.01;Name=MsG0480023661.01
Chr4	mRNA	88233310	88234298	88233310	ID=MsG0480023661.01.T01;Parent=MsG0480023661.01;Name=MsG0480023661.01.T01;_AED=0.42;_eAED=0.42;_QI=39\|1\|0.75\|1\|1\|1\|4\|0\|226
Chr4	exon	88233310	88233432	88233310	ID=MsG0480023661.01.T01:exon:25005;Parent=MsG0480023661.01.T01
Chr4	exon	88233523	88233625	88233523	ID=MsG0480023661.01.T01:exon:25004;Parent=MsG0480023661.01.T01
Chr4	exon	88233715	88233884	88233715	ID=MsG0480023661.01.T01:exon:25003;Parent=MsG0480023661.01.T01
Chr4	exon	88233975	88234298	88233975	ID=MsG0480023661.01.T01:exon:25002;Parent=MsG0480023661.01.T01
Chr4	five_prime_UTR	88234260	88234298	88234260	ID=MsG0480023661.01.T01:five_prime_utr;Parent=MsG0480023661.01.T01
Chr4	CDS	88233975	88234259	88233975	ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01
Chr4	CDS	88233715	88233884	88233715	ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01
Chr4	CDS	88233523	88233625	88233523	ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01
Chr4	CDS	88233310	88233432	88233310	ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01

Gene Sequence

>MsG0480023661.01.T01

ATGGGAAGCTTTGGAATGGAACGTGAAAAACTTGAGATGTTTGAAGTTGGACCATGCATAGATTCATACGAAATGGGTTTCTTAATAGGCCAAAGATTCTCCCAACGGATAAAAAATAGAGTGGCTTCAGACACCATACTTCAAAATCAGCTTCGACCCTTTGCTCAAACACCTCAGTCAGAATCACTTCTCAAAGCACTTTTTGATAATAATCAAACCAAATTCCCTATGTATTGGGATGAACTCTTGGGCACTGCAGCTGGAAGTGGTGTGCCTCTTCTTGATATTTTACTTATTAATTTCAAGAAGGAGATTCTTGCATTTGTTCCGAAAGAAGGATTGAAAAGTTTAGAAGATGATTCCTCAAATGAGTGTTCTACAGTTCTTGTTGTTGGTGAATCTATGGCCATTGCAGCACACAATGAAGATGCAAATGTTGTCCTTGTTGGTCACACCTACTTAATTAAAGGAATATTGCCAAATGGGCTATTCTTTGTTGCTTATACATATGCTGGGGAGCTTCCAAGCTCTGCATTTGCATTTAATAGCAATGGATTGGCATTCACTCTAAATTCAGTACCACCAGCTGAAGATGAAATTGTAGCTGGTGGGATTGGGCGCAACTTCATTTCGCGAGGCATTCTTGAAGCTACTGGCATTGACGATGCTATAAATGTTTGA

Protein sequence

>MsG0480023661.01.T01

MGSFGMEREKLEMFEVGPCIDSYEMGFLIGQRFSQRIKNRVASDTILQNQLRPFAQTPQSESLLKALFDNNQTKFPMYWDELLGTAAGSGVPLLDILLINFKKEILAFVPKEGLKSLEDDSSNECSTVLVVGESMAIAAHNEDANVVLVGHTYLIKGILPNGLFFVAYTYAGELPSSAFAFNSNGLAFTLNSVPPAEDEIVAGGIGRNFISRGILEATGIDDAINV*