AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023661.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023661.01.T01 MTR_8g099160 96.861 223 4 1 6 225 1 223 1.04e-156 439
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGCAGCTACTTAATTAA+AGG 0.212532 4:-88233611 MsG0480023661.01.T01:intron
ATCCCAATACATAGGGAATT+TGG 0.221607 4:+88234020 None:intergenic
TACGAAATGGGTTTCTTAAT+AGG 0.229802 4:-88234171 MsG0480023661.01.T01:CDS
TTATCCGTTGGGAGAATCTT+TGG 0.281279 4:+88234148 None:intergenic
TCTTGTTGTTGGTGAATCTA+TGG 0.342990 4:-88233764 MsG0480023661.01.T01:CDS
ATGCATAGATTCATACGAAA+TGG 0.355370 4:-88234184 MsG0480023661.01.T01:CDS
ACACACACAGGAACGTGAAA+TGG 0.362028 4:-88234256 MsG0480023661.01.T01:exon
GAACTCTTGGGCACTGCAGC+TGG 0.366126 4:-88233997 MsG0480023661.01.T01:CDS
AAACCAAATTCCCTATGTAT+TGG 0.366578 4:-88234023 MsG0480023661.01.T01:CDS
TTTGTTGCTTATACATATGC+TGG 0.369186 4:-88233566 MsG0480023661.01.T01:CDS
AACCAAATTCCCTATGTATT+GGG 0.374000 4:-88234022 MsG0480023661.01.T01:CDS
TGTTCTACAGTTCTTGTTGT+TGG 0.381983 4:-88233775 MsG0480023661.01.T01:CDS
CGAGGCATTCTTGAAGCTAC+TGG 0.388413 4:-88233335 MsG0480023661.01.T01:CDS
CTTGCATTTGTTCCGAAAGA+AGG 0.393434 4:-88233832 MsG0480023661.01.T01:CDS
GAACGTGAAATGGGAAGCTT+TGG 0.397755 4:-88234246 MsG0480023661.01.T01:exon
ATTAAAGGAATATTGCCAAA+TGG 0.406702 4:-88233596 MsG0480023661.01.T01:CDS
GCAACAAAGAATAGCCCATT+TGG 0.413622 4:+88233581 None:intergenic
TATGTATTGGGATGAACTCT+TGG 0.415911 4:-88234010 MsG0480023661.01.T01:CDS
GAAATTGTAGCTGGTGGGAT+TGG 0.433535 4:-88233374 MsG0480023661.01.T01:CDS
GATGCAAATGTTGTCCTTGT+TGG 0.435349 4:-88233721 MsG0480023661.01.T01:CDS
TTAAAGGAATATTGCCAAAT+GGG 0.435577 4:-88233595 MsG0480023661.01.T01:CDS
TGCATTTAATAGCAATGGAT+TGG 0.437443 4:-88233522 MsG0480023661.01.T01:intron
TTGGGCACTGCAGCTGGAAG+TGG 0.442276 4:-88233991 MsG0480023661.01.T01:CDS
GCATTTGCATTTAATAGCAA+TGG 0.449501 4:-88233527 MsG0480023661.01.T01:CDS
TTGTTGCTTATACATATGCT+GGG 0.459480 4:-88233565 MsG0480023661.01.T01:CDS
GACTGAGGTGTTTGAGCAAA+GGG 0.462664 4:+88234081 None:intergenic
AAATTGTAGCTGGTGGGATT+GGG 0.464490 4:-88233373 MsG0480023661.01.T01:CDS
TGCATAGATTCATACGAAAT+GGG 0.467431 4:-88234183 MsG0480023661.01.T01:CDS
CACACACAGGAACGTGAAAT+GGG 0.468759 4:-88234255 MsG0480023661.01.T01:exon
AAACTTGAGATGTTTGAAGT+TGG 0.476023 4:-88234210 MsG0480023661.01.T01:CDS
ATCTTCATTGTGTGCTGCAA+TGG 0.479739 4:+88233741 None:intergenic
TTAAATGCAAATGCAGAGCT+TGG 0.481636 4:+88233536 None:intergenic
TTTGTGTTCATTGTAGAACT+TGG 0.483673 4:-88234285 None:intergenic
AACTGTAGAACACTCATTTG+AGG 0.494365 4:+88233786 None:intergenic
TTTACTTATTAATTTCAAGA+AGG 0.519559 4:-88233860 MsG0480023661.01.T01:CDS
AAGAGTTCATCCCAATACAT+AGG 0.525942 4:+88234012 None:intergenic
ATGTATTGGGATGAACTCTT+GGG 0.531229 4:-88234009 MsG0480023661.01.T01:CDS
GCTACAATTTCATCTTCAGC+TGG 0.536105 4:+88233386 None:intergenic
TGACTGAGGTGTTTGAGCAA+AGG 0.552574 4:+88234080 None:intergenic
AGAGTTCATCCCAATACATA+GGG 0.557026 4:+88234013 None:intergenic
CACGTTCCTGTGTGTGTCTG+TGG 0.562929 4:+88234262 None:intergenic
TGAAATGGGAAGCTTTGGAA+TGG 0.574755 4:-88234241 MsG0480023661.01.T01:exon
AAGATATTTACATCAAGAAG+AGG 0.587862 4:+88233964 None:intergenic
GCTGAAGATGAAATTGTAGC+TGG 0.605512 4:-88233383 MsG0480023661.01.T01:CDS
TTGAGAAGTGATTCTGACTG+AGG 0.618267 4:+88234066 None:intergenic
ATTTCGTATGAATCTATGCA+TGG 0.620039 4:+88234186 None:intergenic
ACAATTTCATCTTCAGCTGG+TGG 0.624043 4:+88233389 None:intergenic
TGTTGCTTATACATATGCTG+GGG 0.624573 4:-88233564 MsG0480023661.01.T01:CDS
GGGCGCAACTTCATTTCGCG+AGG 0.627990 4:-88233353 MsG0480023661.01.T01:CDS
TAGGCCAAAGATTCTCCCAA+CGG 0.638678 4:-88234152 MsG0480023661.01.T01:CDS
ACTTGGCCACAGACACACAC+AGG 0.641350 4:-88234268 MsG0480023661.01.T01:five_prime_UTR
AAGATGAAATTGTAGCTGGT+GGG 0.668109 4:-88233379 MsG0480023661.01.T01:CDS
ACGCTTACGTGTGACCAACA+AGG 0.668777 4:+88233707 None:intergenic
GAAGATGAAATTGTAGCTGG+TGG 0.686068 4:-88233380 MsG0480023661.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTACTTATTAATTTCAAGA+AGG - Chr4:88233726-88233745 MsG0480023661.01.T01:CDS 15.0%
!!! AAGAGATCATTTTAAATAAT+AGG + Chr4:88234099-88234118 None:intergenic 15.0%
!!! AGAGATCATTTTAAATAATA+GGG + Chr4:88234098-88234117 None:intergenic 15.0%
!!! GTTTTGAAATTTGAATAAAA+TGG - Chr4:88233917-88233936 MsG0480023661.01.T01:intron 15.0%
!! TTCTTAATACTTACATTACA+TGG + Chr4:88234132-88234151 None:intergenic 20.0%
!!! AAATTTGCTTTATGATGATT+AGG - Chr4:88234154-88234173 MsG0480023661.01.T01:CDS 20.0%
!!! ATAAGTATTCTTTCCTTTTT+TGG - Chr4:88233944-88233963 MsG0480023661.01.T01:intron 20.0%
! AAGATATTTACATCAAGAAG+AGG + Chr4:88233625-88233644 None:intergenic 25.0%
! ATTAAAGGAATATTGCCAAA+TGG - Chr4:88233990-88234009 MsG0480023661.01.T01:CDS 25.0%
! TATATACAATGCAACAACAT+TGG + Chr4:88233664-88233683 None:intergenic 25.0%
! TTAAAGGAATATTGCCAAAT+GGG - Chr4:88233991-88234010 MsG0480023661.01.T01:CDS 25.0%
! TTATGCAGCTACTTAATTAA+AGG - Chr4:88233975-88233994 MsG0480023661.01.T01:CDS 25.0%
!! AAACTTTTCAATCCTTCTTT+CGG + Chr4:88233769-88233788 None:intergenic 25.0%
AAACCAAATTCCCTATGTAT+TGG - Chr4:88233563-88233582 MsG0480023661.01.T01:CDS 30.0%
AACCAAATTCCCTATGTATT+GGG - Chr4:88233564-88233583 MsG0480023661.01.T01:CDS 30.0%
ATGCATAGATTCATACGAAA+TGG - Chr4:88233402-88233421 MsG0480023661.01.T01:CDS 30.0%
ATTTCGTATGAATCTATGCA+TGG + Chr4:88233403-88233422 None:intergenic 30.0%
CATAACAGAAAGACCAAAAA+AGG + Chr4:88233960-88233979 None:intergenic 30.0%
GCATTTGCATTTAATAGCAA+TGG - Chr4:88234059-88234078 MsG0480023661.01.T01:CDS 30.0%
TGCATAGATTCATACGAAAT+GGG - Chr4:88233403-88233422 MsG0480023661.01.T01:CDS 30.0%
TGCATTTAATAGCAATGGAT+TGG - Chr4:88234064-88234083 MsG0480023661.01.T01:CDS 30.0%
! AAACTTGAGATGTTTGAAGT+TGG - Chr4:88233376-88233395 MsG0480023661.01.T01:CDS 30.0%
! TACGAAATGGGTTTCTTAAT+AGG - Chr4:88233415-88233434 MsG0480023661.01.T01:intron 30.0%
! TTGTTGCTTATACATATGCT+GGG - Chr4:88234021-88234040 MsG0480023661.01.T01:CDS 30.0%
! TTTGTTGCTTATACATATGC+TGG - Chr4:88234020-88234039 MsG0480023661.01.T01:CDS 30.0%
!!! CACTCTATTTTTTATCCGTT+GGG + Chr4:88233452-88233471 None:intergenic 30.0%
AACTGTAGAACACTCATTTG+AGG + Chr4:88233803-88233822 None:intergenic 35.0%
AAGAGTTCATCCCAATACAT+AGG + Chr4:88233577-88233596 None:intergenic 35.0%
AAGATGAAATTGTAGCTGGT+GGG - Chr4:88234207-88234226 MsG0480023661.01.T01:CDS 35.0%
AGAGTTCATCCCAATACATA+GGG + Chr4:88233576-88233595 None:intergenic 35.0%
ATCCCAATACATAGGGAATT+TGG + Chr4:88233569-88233588 None:intergenic 35.0%
ATGTATTGGGATGAACTCTT+GGG - Chr4:88233577-88233596 MsG0480023661.01.T01:CDS 35.0%
CCAACGGATAAAAAATAGAG+TGG - Chr4:88233450-88233469 MsG0480023661.01.T01:intron 35.0%
TATGTATTGGGATGAACTCT+TGG - Chr4:88233576-88233595 MsG0480023661.01.T01:CDS 35.0%
TTAAATGCAAATGCAGAGCT+TGG + Chr4:88234053-88234072 None:intergenic 35.0%
! TGTTCTACAGTTCTTGTTGT+TGG - Chr4:88233811-88233830 MsG0480023661.01.T01:CDS 35.0%
! TGTTGCTTATACATATGCTG+GGG - Chr4:88234022-88234041 MsG0480023661.01.T01:CDS 35.0%
!! TCTTGTTGTTGGTGAATCTA+TGG - Chr4:88233822-88233841 MsG0480023661.01.T01:CDS 35.0%
!!! CCACTCTATTTTTTATCCGT+TGG + Chr4:88233453-88233472 None:intergenic 35.0%
!!! TCGAAGCTGATTTTGAAGTA+TGG + Chr4:88233485-88233504 None:intergenic 35.0%
AAATTGTAGCTGGTGGGATT+GGG - Chr4:88234213-88234232 MsG0480023661.01.T01:CDS 40.0%
ACAATTTCATCTTCAGCTGG+TGG + Chr4:88234200-88234219 None:intergenic 40.0%
ATCTTCATTGTGTGCTGCAA+TGG + Chr4:88233848-88233867 None:intergenic 40.0%
CTTGCATTTGTTCCGAAAGA+AGG - Chr4:88233754-88233773 MsG0480023661.01.T01:CDS 40.0%
GAAGATGAAATTGTAGCTGG+TGG - Chr4:88234206-88234225 MsG0480023661.01.T01:CDS 40.0%
GATGCAAATGTTGTCCTTGT+TGG - Chr4:88233865-88233884 MsG0480023661.01.T01:CDS 40.0%
GCAACAAAGAATAGCCCATT+TGG + Chr4:88234008-88234027 None:intergenic 40.0%
GCTACAATTTCATCTTCAGC+TGG + Chr4:88234203-88234222 None:intergenic 40.0%
GCTGAAGATGAAATTGTAGC+TGG - Chr4:88234203-88234222 MsG0480023661.01.T01:CDS 40.0%
TTATCCGTTGGGAGAATCTT+TGG + Chr4:88233441-88233460 None:intergenic 40.0%
TTGAGAAGTGATTCTGACTG+AGG + Chr4:88233523-88233542 None:intergenic 40.0%
!! TGAAATGGGAAGCTTTGGAA+TGG - Chr4:88233345-88233364 MsG0480023661.01.T01:CDS 40.0%
ACACACACAGGAACGTGAAA+TGG - Chr4:88233330-88233349 MsG0480023661.01.T01:CDS 45.0%
CACACACAGGAACGTGAAAT+GGG - Chr4:88233331-88233350 MsG0480023661.01.T01:CDS 45.0%
GAAATTGTAGCTGGTGGGAT+TGG - Chr4:88234212-88234231 MsG0480023661.01.T01:CDS 45.0%
GAACGTGAAATGGGAAGCTT+TGG - Chr4:88233340-88233359 MsG0480023661.01.T01:CDS 45.0%
TAGGCCAAAGATTCTCCCAA+CGG - Chr4:88233434-88233453 MsG0480023661.01.T01:intron 45.0%
! GACTGAGGTGTTTGAGCAAA+GGG + Chr4:88233508-88233527 None:intergenic 45.0%
! TGACTGAGGTGTTTGAGCAA+AGG + Chr4:88233509-88233528 None:intergenic 45.0%
ACGCTTACGTGTGACCAACA+AGG + Chr4:88233882-88233901 None:intergenic 50.0%
! CGAGGCATTCTTGAAGCTAC+TGG - Chr4:88234251-88234270 MsG0480023661.01.T01:exon 50.0%
ACTTGGCCACAGACACACAC+AGG - Chr4:88233318-88233337 MsG0480023661.01.T01:CDS 55.0%
CACGTTCCTGTGTGTGTCTG+TGG + Chr4:88233327-88233346 None:intergenic 55.0%
GGGCGCAACTTCATTTCGCG+AGG - Chr4:88234233-88234252 MsG0480023661.01.T01:CDS 60.0%
! GAACTCTTGGGCACTGCAGC+TGG - Chr4:88233589-88233608 MsG0480023661.01.T01:CDS 60.0%
! TTGGGCACTGCAGCTGGAAG+TGG - Chr4:88233595-88233614 MsG0480023661.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 88233310 88234298 88233310 ID=MsG0480023661.01;Name=MsG0480023661.01
Chr4 mRNA 88233310 88234298 88233310 ID=MsG0480023661.01.T01;Parent=MsG0480023661.01;Name=MsG0480023661.01.T01;_AED=0.42;_eAED=0.42;_QI=39|1|0.75|1|1|1|4|0|226
Chr4 exon 88233310 88233432 88233310 ID=MsG0480023661.01.T01:exon:25005;Parent=MsG0480023661.01.T01
Chr4 exon 88233523 88233625 88233523 ID=MsG0480023661.01.T01:exon:25004;Parent=MsG0480023661.01.T01
Chr4 exon 88233715 88233884 88233715 ID=MsG0480023661.01.T01:exon:25003;Parent=MsG0480023661.01.T01
Chr4 exon 88233975 88234298 88233975 ID=MsG0480023661.01.T01:exon:25002;Parent=MsG0480023661.01.T01
Chr4 five_prime_UTR 88234260 88234298 88234260 ID=MsG0480023661.01.T01:five_prime_utr;Parent=MsG0480023661.01.T01
Chr4 CDS 88233975 88234259 88233975 ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01
Chr4 CDS 88233715 88233884 88233715 ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01
Chr4 CDS 88233523 88233625 88233523 ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01
Chr4 CDS 88233310 88233432 88233310 ID=MsG0480023661.01.T01:cds;Parent=MsG0480023661.01.T01
Gene Sequence

>MsG0480023661.01.T01

ATGGGAAGCTTTGGAATGGAACGTGAAAAACTTGAGATGTTTGAAGTTGGACCATGCATAGATTCATACGAAATGGGTTTCTTAATAGGCCAAAGATTCTCCCAACGGATAAAAAATAGAGTGGCTTCAGACACCATACTTCAAAATCAGCTTCGACCCTTTGCTCAAACACCTCAGTCAGAATCACTTCTCAAAGCACTTTTTGATAATAATCAAACCAAATTCCCTATGTATTGGGATGAACTCTTGGGCACTGCAGCTGGAAGTGGTGTGCCTCTTCTTGATATTTTACTTATTAATTTCAAGAAGGAGATTCTTGCATTTGTTCCGAAAGAAGGATTGAAAAGTTTAGAAGATGATTCCTCAAATGAGTGTTCTACAGTTCTTGTTGTTGGTGAATCTATGGCCATTGCAGCACACAATGAAGATGCAAATGTTGTCCTTGTTGGTCACACCTACTTAATTAAAGGAATATTGCCAAATGGGCTATTCTTTGTTGCTTATACATATGCTGGGGAGCTTCCAAGCTCTGCATTTGCATTTAATAGCAATGGATTGGCATTCACTCTAAATTCAGTACCACCAGCTGAAGATGAAATTGTAGCTGGTGGGATTGGGCGCAACTTCATTTCGCGAGGCATTCTTGAAGCTACTGGCATTGACGATGCTATAAATGTTTGA

Protein sequence

>MsG0480023661.01.T01

MGSFGMEREKLEMFEVGPCIDSYEMGFLIGQRFSQRIKNRVASDTILQNQLRPFAQTPQSESLLKALFDNNQTKFPMYWDELLGTAAGSGVPLLDILLINFKKEILAFVPKEGLKSLEDDSSNECSTVLVVGESMAIAAHNEDANVVLVGHTYLIKGILPNGLFFVAYTYAGELPSSAFAFNSNGLAFTLNSVPPAEDEIVAGGIGRNFISRGILEATGIDDAINV*