Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015008.01.T01 | XP_013460468.1 | 94.196 | 224 | 13 | 0 | 1 | 224 | 1 | 224 | 1.87E-155 | 440 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015008.01.T01 | P25317 | 71.233 | 219 | 63 | 0 | 5 | 223 | 1 | 219 | 3.70E-119 | 340 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015008.01.T01 | I3S5Q9 | 94.196 | 224 | 13 | 0 | 1 | 224 | 1 | 224 | 8.91e-156 | 440 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004154.01 | MsG0380015008.01 | 0.847126 | 1.408301e-59 | 2.478563e-56 |
| MsG0180004266.01 | MsG0380015008.01 | 0.874932 | 4.586357e-68 | 2.102917e-64 |
| MsG0180004941.01 | MsG0380015008.01 | 0.823903 | 1.077836e-53 | 9.403695e-51 |
| MsG0180005542.01 | MsG0380015008.01 | 0.802682 | 4.997255e-49 | 2.463115e-46 |
| MsG0280011165.01 | MsG0380015008.01 | 0.837927 | 3.892853e-57 | 5.133481e-54 |
| MsG0280011193.01 | MsG0380015008.01 | 0.835200 | 1.927459e-56 | 2.340276e-53 |
| MsG0280011203.01 | MsG0380015008.01 | 0.847329 | 1.238729e-59 | 2.193895e-56 |
| MsG0280011264.01 | MsG0380015008.01 | 0.807048 | 6.114031e-50 | 3.371035e-47 |
| MsG0380015008.01 | MsG0380015009.01 | 0.902686 | 7.520423e-79 | 1.077673e-74 |
| MsG0380015008.01 | MsG0380015010.01 | 0.853974 | 1.676100e-61 | 3.686837e-58 |
| MsG0380015008.01 | MsG0380016801.01 | 0.801059 | 1.076631e-48 | 5.092913e-46 |
| MsG0380015008.01 | MsG0480019228.01 | 0.809316 | 2.009670e-50 | 1.176101e-47 |
| MsG0380015008.01 | MsG0480022032.01 | 0.813843 | 2.084953e-51 | 1.376556e-48 |
| MsG0380015008.01 | MsG0480022655.01 | 0.809831 | 1.557440e-50 | 9.240153e-48 |
| MsG0380015008.01 | MsG0480023267.01 | 0.802836 | 4.643296e-49 | 2.297869e-46 |
| MsG0380015008.01 | MsG0480023303.01 | 0.803251 | 3.810721e-49 | 1.905788e-46 |
| MsG0380015008.01 | MsG0480023921.01 | 0.800097 | 1.690815e-48 | 7.804972e-46 |
| MsG0380015008.01 | MsG0580030135.01 | 0.808623 | 2.827655e-50 | 1.624530e-47 |
| MsG0380015008.01 | MsG0680031240.01 | 0.821200 | 4.582768e-53 | 3.703940e-50 |
| MsG0380015008.01 | MsG0680034317.01 | 0.806341 | 8.622492e-50 | 4.668427e-47 |
| MsG0380015008.01 | MsG0780036661.01 | 0.824853 | 6.443222e-54 | 5.776211e-51 |
| MsG0380015008.01 | MsG0780039978.01 | 0.804002 | 2.662108e-49 | 1.357352e-46 |
| MsG0380015008.01 | MsG0780041583.01 | 0.814771 | 1.299806e-51 | 8.799895e-49 |
| MsG0380015008.01 | MsG0880043744.01 | 0.827078 | 1.907791e-54 | 1.822609e-51 |
| MsG0380015008.01 | MsG0880045818.01 | 0.835615 | 1.513325e-56 | 1.860398e-53 |
| MsG0280007914.01 | MsG0380015008.01 | 0.837223 | 5.899863e-57 | 7.612745e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015008.01.T01 | MTR_3g467420 | 94.196 | 224 | 13 | 0 | 1 | 224 | 1 | 224 | 2.26e-159 | 440 |
| MsG0380015008.01.T01 | MTR_3g467430 | 89.286 | 224 | 24 | 0 | 1 | 224 | 1 | 224 | 6.69e-150 | 416 |
| MsG0380015008.01.T01 | MTR_2g070180 | 63.333 | 210 | 77 | 0 | 10 | 219 | 5 | 214 | 5.37e-103 | 297 |
| MsG0380015008.01.T01 | MTR_2g070150 | 67.000 | 200 | 66 | 0 | 10 | 209 | 5 | 204 | 2.81e-102 | 295 |
| MsG0380015008.01.T01 | MTR_2g070140 | 66.010 | 203 | 69 | 0 | 22 | 224 | 1 | 203 | 4.08e-102 | 294 |
| MsG0380015008.01.T01 | MTR_2g070130 | 61.860 | 215 | 82 | 0 | 10 | 224 | 5 | 219 | 1.80e-100 | 291 |
| MsG0380015008.01.T01 | MTR_2g070210 | 63.134 | 217 | 78 | 1 | 10 | 224 | 5 | 221 | 3.36e-99 | 288 |
| MsG0380015008.01.T01 | MTR_2g070060 | 60.000 | 215 | 86 | 0 | 10 | 224 | 5 | 219 | 8.09e-98 | 284 |
| MsG0380015008.01.T01 | MTR_2g070200 | 63.594 | 217 | 77 | 2 | 10 | 224 | 5 | 221 | 6.49e-95 | 276 |
| MsG0380015008.01.T01 | MTR_2g070070 | 60.829 | 217 | 85 | 0 | 8 | 224 | 3 | 219 | 2.43e-92 | 270 |
| MsG0380015008.01.T01 | MTR_2g070110 | 59.908 | 217 | 86 | 1 | 8 | 224 | 3 | 218 | 3.56e-90 | 265 |
| MsG0380015008.01.T01 | MTR_8g056940 | 54.091 | 220 | 100 | 1 | 5 | 224 | 1 | 219 | 3.63e-88 | 259 |
| MsG0380015008.01.T01 | MTR_2g070120 | 57.075 | 212 | 91 | 0 | 10 | 221 | 5 | 216 | 1.22e-87 | 258 |
| MsG0380015008.01.T01 | MTR_4g124130 | 55.251 | 219 | 97 | 1 | 7 | 224 | 2 | 220 | 1.67e-86 | 255 |
| MsG0380015008.01.T01 | MTR_5g076900 | 50.901 | 222 | 106 | 3 | 5 | 224 | 1 | 221 | 4.75e-79 | 236 |
| MsG0380015008.01.T01 | MTR_0186s0030 | 55.634 | 142 | 62 | 1 | 5 | 146 | 1 | 141 | 1.41e-53 | 169 |
| MsG0380015008.01.T01 | MTR_8g087425 | 39.640 | 222 | 130 | 3 | 5 | 223 | 1 | 221 | 1.25e-46 | 154 |
| MsG0380015008.01.T01 | MTR_8g061950 | 38.197 | 233 | 127 | 5 | 5 | 223 | 1 | 230 | 2.78e-46 | 153 |
| MsG0380015008.01.T01 | MTR_7g065720 | 41.827 | 208 | 115 | 5 | 10 | 213 | 8 | 213 | 2.90e-46 | 153 |
| MsG0380015008.01.T01 | MTR_5g037380 | 41.704 | 223 | 115 | 5 | 5 | 215 | 1 | 220 | 4.41e-46 | 152 |
| MsG0380015008.01.T01 | MTR_1g090060 | 40.845 | 213 | 119 | 4 | 5 | 213 | 1 | 210 | 4.82e-46 | 152 |
| MsG0380015008.01.T01 | MTR_7g065270 | 44.388 | 196 | 103 | 6 | 18 | 210 | 16 | 208 | 5.63e-46 | 152 |
| MsG0380015008.01.T01 | MTR_7g065660 | 39.423 | 208 | 121 | 4 | 9 | 213 | 7 | 212 | 7.83e-45 | 149 |
| MsG0380015008.01.T01 | MTR_3g099757 | 42.788 | 208 | 116 | 3 | 5 | 210 | 1 | 207 | 1.66e-44 | 148 |
| MsG0380015008.01.T01 | MTR_7g065740 | 39.336 | 211 | 122 | 4 | 7 | 213 | 5 | 213 | 3.94e-44 | 147 |
| MsG0380015008.01.T01 | MTR_8g087410 | 41.872 | 203 | 115 | 2 | 5 | 205 | 2 | 203 | 6.16e-44 | 147 |
| MsG0380015008.01.T01 | MTR_7g065265 | 44.221 | 199 | 106 | 5 | 18 | 213 | 16 | 212 | 2.05e-43 | 145 |
| MsG0380015008.01.T01 | MTR_4g019790 | 38.571 | 210 | 124 | 4 | 7 | 213 | 5 | 212 | 4.73e-43 | 145 |
| MsG0380015008.01.T01 | MTR_4g019780 | 38.426 | 216 | 123 | 6 | 5 | 213 | 1 | 213 | 1.57e-42 | 143 |
| MsG0380015008.01.T01 | MTR_7g065290 | 42.211 | 199 | 110 | 5 | 18 | 213 | 16 | 212 | 2.00e-42 | 143 |
| MsG0380015008.01.T01 | MTR_7g065590 | 41.206 | 199 | 112 | 5 | 18 | 213 | 16 | 212 | 4.34e-42 | 142 |
| MsG0380015008.01.T01 | MTR_7g065630 | 39.234 | 209 | 122 | 5 | 6 | 213 | 22 | 226 | 9.74e-42 | 142 |
| MsG0380015008.01.T01 | MTR_5g040430 | 33.971 | 209 | 130 | 3 | 8 | 209 | 6 | 213 | 2.82e-41 | 140 |
| MsG0380015008.01.T01 | MTR_4g059730 | 39.901 | 203 | 117 | 3 | 15 | 213 | 10 | 211 | 4.30e-41 | 139 |
| MsG0380015008.01.T01 | MTR_7g065750 | 38.710 | 217 | 122 | 6 | 10 | 219 | 8 | 220 | 5.35e-41 | 139 |
| MsG0380015008.01.T01 | MTR_1g090150 | 39.320 | 206 | 118 | 5 | 9 | 210 | 3 | 205 | 1.43e-40 | 138 |
| MsG0380015008.01.T01 | MTR_7g065230 | 41.667 | 204 | 110 | 6 | 10 | 211 | 8 | 204 | 1.51e-40 | 138 |
| MsG0380015008.01.T01 | MTR_7g065600 | 39.500 | 200 | 116 | 4 | 9 | 205 | 7 | 204 | 2.78e-40 | 137 |
| MsG0380015008.01.T01 | MTR_1g090100 | 39.048 | 210 | 123 | 3 | 9 | 214 | 2 | 210 | 5.33e-40 | 137 |
| MsG0380015008.01.T01 | MTR_7g065710 | 38.579 | 197 | 116 | 5 | 18 | 213 | 13 | 205 | 1.01e-39 | 136 |
| MsG0380015008.01.T01 | MTR_6g080440 | 37.438 | 203 | 119 | 5 | 23 | 219 | 17 | 217 | 4.69e-39 | 134 |
| MsG0380015008.01.T01 | MTR_1g090070 | 36.073 | 219 | 134 | 4 | 1 | 214 | 1 | 218 | 6.90e-39 | 134 |
| MsG0380015008.01.T01 | MTR_7g065260 | 43.455 | 191 | 103 | 5 | 18 | 205 | 23 | 211 | 6.34e-38 | 132 |
| MsG0380015008.01.T01 | MTR_7g065700 | 40.385 | 208 | 119 | 5 | 7 | 213 | 5 | 208 | 7.96e-38 | 131 |
| MsG0380015008.01.T01 | MTR_7g065680 | 40.102 | 197 | 113 | 5 | 18 | 213 | 16 | 208 | 3.73e-37 | 129 |
| MsG0380015008.01.T01 | MTR_1g090090 | 38.967 | 213 | 123 | 5 | 5 | 213 | 1 | 210 | 8.50e-37 | 129 |
| MsG0380015008.01.T01 | MTR_1g115195 | 37.156 | 218 | 130 | 6 | 8 | 220 | 2 | 217 | 1.22e-35 | 125 |
| MsG0380015008.01.T01 | MTR_7g065640 | 34.466 | 206 | 116 | 4 | 9 | 213 | 7 | 194 | 3.69e-34 | 121 |
| MsG0380015008.01.T01 | MTR_2g072120 | 60.294 | 68 | 27 | 0 | 52 | 119 | 1 | 68 | 4.52e-25 | 94.4 |
| MsG0380015008.01.T01 | MTR_7g100320 | 28.718 | 195 | 128 | 7 | 20 | 210 | 37 | 224 | 2.24e-14 | 70.1 |
| MsG0380015008.01.T01 | MTR_4g043640 | 57.143 | 42 | 18 | 0 | 153 | 194 | 44 | 85 | 9.34e-13 | 62.4 |
| MsG0380015008.01.T01 | MTR_1g067180 | 27.922 | 154 | 102 | 6 | 59 | 210 | 76 | 222 | 8.55e-11 | 60.1 |
| MsG0380015008.01.T01 | MTR_1g067180 | 27.922 | 154 | 102 | 6 | 59 | 210 | 79 | 225 | 9.82e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015008.01.T01 | AT1G17180 | 65.581 | 215 | 74 | 0 | 10 | 224 | 5 | 219 | 9.03e-105 | 301 |
| MsG0380015008.01.T01 | AT1G78370 | 62.085 | 211 | 80 | 0 | 11 | 221 | 6 | 216 | 3.43e-102 | 295 |
| MsG0380015008.01.T01 | AT1G78380 | 61.972 | 213 | 81 | 0 | 8 | 220 | 3 | 215 | 1.07e-100 | 291 |
| MsG0380015008.01.T01 | AT1G17170 | 63.721 | 215 | 77 | 1 | 10 | 224 | 5 | 218 | 5.37e-99 | 287 |
| MsG0380015008.01.T01 | AT1G78360 | 60.092 | 218 | 86 | 1 | 8 | 224 | 3 | 220 | 1.50e-95 | 278 |
| MsG0380015008.01.T01 | AT1G78340 | 57.619 | 210 | 89 | 0 | 10 | 219 | 5 | 214 | 3.65e-92 | 270 |
| MsG0380015008.01.T01 | AT1G53680 | 56.682 | 217 | 92 | 2 | 10 | 224 | 8 | 224 | 1.60e-89 | 263 |
| MsG0380015008.01.T01 | AT1G78320 | 57.746 | 213 | 90 | 0 | 7 | 219 | 2 | 214 | 5.46e-88 | 259 |
| MsG0380015008.01.T01 | AT1G17190 | 53.211 | 218 | 102 | 0 | 5 | 222 | 1 | 218 | 1.21e-82 | 246 |
| MsG0380015008.01.T01 | AT3G43800 | 52.941 | 221 | 100 | 3 | 5 | 221 | 1 | 221 | 9.03e-82 | 244 |
| MsG0380015008.01.T01 | AT1G78320 | 50.235 | 213 | 79 | 1 | 7 | 219 | 2 | 187 | 3.28e-70 | 213 |
| MsG0380015008.01.T01 | AT2G29420 | 41.063 | 207 | 119 | 3 | 1 | 205 | 1 | 206 | 4.13e-50 | 163 |
| MsG0380015008.01.T01 | AT1G10360 | 42.202 | 218 | 118 | 4 | 5 | 214 | 1 | 218 | 4.10e-49 | 160 |
| MsG0380015008.01.T01 | AT3G09270 | 39.130 | 207 | 123 | 2 | 7 | 210 | 4 | 210 | 8.89e-46 | 152 |
| MsG0380015008.01.T01 | AT1G74590 | 36.697 | 218 | 130 | 4 | 10 | 220 | 8 | 224 | 4.30e-45 | 150 |
| MsG0380015008.01.T01 | AT1G10370 | 40.444 | 225 | 112 | 5 | 5 | 214 | 1 | 218 | 5.34e-45 | 150 |
| MsG0380015008.01.T01 | AT1G59700 | 39.906 | 213 | 119 | 4 | 10 | 213 | 7 | 219 | 2.80e-43 | 145 |
| MsG0380015008.01.T01 | AT5G62480 | 38.605 | 215 | 119 | 4 | 6 | 209 | 5 | 217 | 5.36e-43 | 145 |
| MsG0380015008.01.T01 | AT1G27130 | 36.036 | 222 | 132 | 5 | 5 | 216 | 1 | 222 | 7.13e-42 | 142 |
| MsG0380015008.01.T01 | AT1G69930 | 37.264 | 212 | 126 | 3 | 10 | 214 | 13 | 224 | 7.18e-42 | 142 |
| MsG0380015008.01.T01 | AT2G29440 | 41.379 | 203 | 107 | 7 | 10 | 205 | 7 | 204 | 5.88e-41 | 139 |
| MsG0380015008.01.T01 | AT1G59670 | 38.636 | 220 | 126 | 5 | 10 | 220 | 7 | 226 | 6.43e-41 | 139 |
| MsG0380015008.01.T01 | AT2G29490 | 37.379 | 206 | 125 | 2 | 7 | 209 | 5 | 209 | 2.27e-40 | 138 |
| MsG0380015008.01.T01 | AT2G29480 | 38.424 | 203 | 119 | 4 | 7 | 205 | 5 | 205 | 2.52e-40 | 138 |
| MsG0380015008.01.T01 | AT2G29450 | 38.739 | 222 | 121 | 8 | 5 | 217 | 1 | 216 | 1.19e-39 | 136 |
| MsG0380015008.01.T01 | AT1G27140 | 34.821 | 224 | 133 | 6 | 5 | 216 | 1 | 223 | 2.14e-38 | 133 |
| MsG0380015008.01.T01 | AT2G29470 | 37.019 | 208 | 124 | 5 | 7 | 209 | 5 | 210 | 3.08e-37 | 130 |
| MsG0380015008.01.T01 | AT2G29460 | 36.946 | 203 | 122 | 4 | 7 | 205 | 5 | 205 | 1.62e-35 | 125 |
| MsG0380015008.01.T01 | AT1G69920 | 30.045 | 223 | 147 | 4 | 1 | 214 | 26 | 248 | 2.81e-32 | 118 |
| MsG0380015008.01.T01 | AT5G62480 | 30.698 | 215 | 110 | 5 | 6 | 209 | 5 | 191 | 4.49e-24 | 95.9 |
| MsG0380015008.01.T01 | AT5G62480 | 30.698 | 215 | 109 | 5 | 6 | 209 | 5 | 190 | 5.48e-24 | 95.5 |
| MsG0380015008.01.T01 | AT5G02790 | 30.065 | 153 | 100 | 4 | 59 | 210 | 80 | 226 | 3.48e-11 | 61.2 |
Find 53 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGACAAAACATATTT+TGG | 0.170928 | 3:+63879756 | MsG0380015008.01.T01:CDS |
| AGCGATCACAAGCCAAGTTT+TGG | 0.291618 | 3:+63878819 | MsG0380015008.01.T01:CDS |
| CCTCACCCTCACAAAATCTA+TGG | 0.311690 | 3:+63879939 | MsG0380015008.01.T01:CDS |
| CTCTTATACCCTTCACTAGT+TGG | 0.329236 | 3:+63879808 | MsG0380015008.01.T01:CDS |
| CTAAACAATGAGTCAATATA+TGG | 0.352448 | 3:+63878526 | MsG0380015008.01.T01:exon |
| TTGGCTTGTGATCGCTTGTA+AGG | 0.379033 | 3:-63878812 | None:intergenic |
| ATGTCCTAAGCTTGTGGCTT+GGG | 0.384787 | 3:+63879875 | MsG0380015008.01.T01:CDS |
| TTTCCATTATGAATCAAAAC+GGG | 0.386030 | 3:-63878710 | None:intergenic |
| GGACTTGAATAGAGATCGTT+AGG | 0.388810 | 3:+63879990 | MsG0380015008.01.T01:exon |
| ATATTAAGTGATTCACATAT+AGG | 0.400119 | 3:-63878734 | None:intergenic |
| TTGGCCCAAGCCACAAGCTT+AGG | 0.419595 | 3:-63879879 | None:intergenic |
| GAATGCTTGAAGACTCTAGA+AGG | 0.458394 | 3:+63879729 | MsG0380015008.01.T01:CDS |
| AGCTTGTGGCTTGGGCCAAA+AGG | 0.465671 | 3:+63879883 | MsG0380015008.01.T01:CDS |
| TTGAGTACATTGATGAAGCT+TGG | 0.466341 | 3:+63878759 | MsG0380015008.01.T01:CDS |
| TTTACACTTACGAGACATAT+GGG | 0.474634 | 3:+63879832 | MsG0380015008.01.T01:CDS |
| GATATACAACATTGGAAAGA+AGG | 0.483460 | 3:+63879656 | MsG0380015008.01.T01:intron |
| AATGTAATCTCCCCAAAACT+TGG | 0.487995 | 3:-63878831 | None:intergenic |
| ACTCTCATTCCATATGAGCT+TGG | 0.497119 | 3:-63878581 | None:intergenic |
| AAGACTCTAGAAGGTGAGCT+TGG | 0.503893 | 3:+63879738 | MsG0380015008.01.T01:CDS |
| GGCCAAAAGGTGTATGGAAA+AGG | 0.504837 | 3:+63879896 | MsG0380015008.01.T01:CDS |
| CTGCACACGAAGTTCTTCTC+AGG | 0.509673 | 3:-63880026 | None:intergenic |
| AGAGATCGTTAGGGATTGCA+TGG | 0.512901 | 3:+63880000 | MsG0380015008.01.T01:exon |
| AAAATTGCATTGGAAGAGAA+GGG | 0.514397 | 3:+63878605 | MsG0380015008.01.T01:CDS |
| AAGAAGGTATGGACAGGAAA+GGG | 0.519335 | 3:+63879672 | MsG0380015008.01.T01:CDS |
| ATTATATTATAATAAACCAT+TGG | 0.531512 | 3:+63880209 | MsG0380015008.01.T01:three_prime_UTR |
| AATGAGAGTGAAAATTGCAT+TGG | 0.532849 | 3:+63878595 | MsG0380015008.01.T01:CDS |
| AATGTCCTAAGCTTGTGGCT+TGG | 0.535005 | 3:+63879874 | MsG0380015008.01.T01:CDS |
| ACAACATTGGAAAGAAGGTA+TGG | 0.535319 | 3:+63879661 | MsG0380015008.01.T01:CDS |
| AAGGGTGAAGAACAAGAAGA+AGG | 0.543295 | 3:+63879690 | MsG0380015008.01.T01:CDS |
| TGACTTTGGGTATGTTGATG+TGG | 0.546781 | 3:+63879785 | MsG0380015008.01.T01:CDS |
| GAAAATTGCATTGGAAGAGA+AGG | 0.550919 | 3:+63878604 | MsG0380015008.01.T01:CDS |
| GGCTTGGGCCAAAAGGTGTA+TGG | 0.551448 | 3:+63879890 | MsG0380015008.01.T01:CDS |
| AATGAGTCAATATATGGCTG+AGG | 0.555908 | 3:+63878532 | MsG0380015008.01.T01:exon |
| AGTGTAAAACCAACTAGTGA+AGG | 0.558553 | 3:-63879817 | None:intergenic |
| ATTGGAAAGAAGGTATGGAC+AGG | 0.560806 | 3:+63879666 | MsG0380015008.01.T01:CDS |
| TGATCGCTTGTAAGGGTCAG+AGG | 0.571746 | 3:-63878804 | None:intergenic |
| GTTTATTATAATATAATCAG+TGG | 0.583859 | 3:-63880203 | None:intergenic |
| GGGTCAGAGGGCAAAAGAGA+AGG | 0.589806 | 3:-63878791 | None:intergenic |
| TGGCTTGTGATCGCTTGTAA+GGG | 0.589840 | 3:-63878811 | None:intergenic |
| GAAAGAATGTCCTAAGCTTG+TGG | 0.598040 | 3:+63879869 | MsG0380015008.01.T01:CDS |
| GTGAGGGTGAGGAAGTGACT+TGG | 0.599429 | 3:-63879928 | None:intergenic |
| GACTTGAATAGAGATCGTTA+GGG | 0.602022 | 3:+63879991 | MsG0380015008.01.T01:exon |
| ATGAGTCAATATATGGCTGA+GGG | 0.603761 | 3:+63878533 | MsG0380015008.01.T01:CDS |
| AAAGAAGGTATGGACAGGAA+AGG | 0.623553 | 3:+63879671 | MsG0380015008.01.T01:CDS |
| GTGTAAAACCAACTAGTGAA+GGG | 0.625155 | 3:-63879816 | None:intergenic |
| TTGATGTAGATATACAACAT+TGG | 0.625837 | 3:+63879648 | MsG0380015008.01.T01:intron |
| GTGTATGGAAAAGGAGAGTG+TGG | 0.633165 | 3:+63879905 | MsG0380015008.01.T01:CDS |
| TTCCATTATGAATCAAAACG+GGG | 0.641712 | 3:-63878709 | None:intergenic |
| GATCGCTTGTAAGGGTCAGA+GGG | 0.649504 | 3:-63878803 | None:intergenic |
| GAGATCGTTAGGGATTGCAT+GGG | 0.652013 | 3:+63880001 | MsG0380015008.01.T01:exon |
| AAATTGCATTGGAAGAGAAG+GGG | 0.654003 | 3:+63878606 | MsG0380015008.01.T01:CDS |
| AATTGCATTGGAAGAGAAGG+GGG | 0.673032 | 3:+63878607 | MsG0380015008.01.T01:CDS |
| ATATATGGCTGAGGGCAATG+TGG | 0.718578 | 3:+63878541 | MsG0380015008.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATATTATAATAAACCAT+TGG | + | Chr3:63880209-63880228 | MsG0380015008.01.T01:three_prime_UTR | 10.0% |
| !!! | AGTTTATGTTTTTTTTTTTA+AGG | + | Chr3:63880094-63880113 | MsG0380015008.01.T01:three_prime_UTR | 10.0% |
| !!! | CTTTAAAAAAATTATATGTT+TGG | - | Chr3:63879585-63879604 | None:intergenic | 10.0% |
| !!! | TTTAATAGATAGATTTTTAT+AGG | + | Chr3:63878995-63879014 | MsG0380015008.01.T01:intron | 10.0% |
| !! | GTTTATTATAATATAATCAG+TGG | - | Chr3:63880206-63880225 | None:intergenic | 15.0% |
| !!! | AAATATTGGTAGTTTAATAA+GGG | + | Chr3:63879019-63879038 | MsG0380015008.01.T01:intron | 15.0% |
| !!! | AACTTATTTTACATTAACAA+TGG | - | Chr3:63880078-63880097 | None:intergenic | 15.0% |
| !! | ATAAGCTGATAAAAAAAAGA+AGG | + | Chr3:63880172-63880191 | MsG0380015008.01.T01:three_prime_UTR | 20.0% |
| !! | ATATTAAGTGATTCACATAT+AGG | - | Chr3:63878737-63878756 | None:intergenic | 20.0% |
| !! | CAAATATGCTACAATATTTA+TGG | - | Chr3:63879423-63879442 | None:intergenic | 20.0% |
| !! | CAATTTACAATTTACATAAG+AGG | + | Chr3:63879513-63879532 | MsG0380015008.01.T01:intron | 20.0% |
| !! | CATAAATATTGTAGCATATT+TGG | + | Chr3:63879421-63879440 | MsG0380015008.01.T01:intron | 20.0% |
| !! | TATCTACTTAAGCATTAATA+TGG | - | Chr3:63879310-63879329 | None:intergenic | 20.0% |
| !! | TTCATAAAGATTGTCTATAA+AGG | - | Chr3:63879142-63879161 | None:intergenic | 20.0% |
| !!! | AATATTGGTAGTTTAATAAG+GGG | + | Chr3:63879020-63879039 | MsG0380015008.01.T01:intron | 20.0% |
| !!! | AGATTTTTATAGGCAAATAT+TGG | + | Chr3:63879005-63879024 | MsG0380015008.01.T01:intron | 20.0% |
| !!! | CAAATATTGGTAGTTTAATA+AGG | + | Chr3:63879018-63879037 | MsG0380015008.01.T01:intron | 20.0% |
| !!! | TCAAGTCTTGATTATTTTTT+TGG | + | Chr3:63879165-63879184 | MsG0380015008.01.T01:intron | 20.0% |
| !!! | TTTTAAAGAGTTGATTCATA+GGG | + | Chr3:63879597-63879616 | MsG0380015008.01.T01:intron | 20.0% |
| !!! | TTTTCCATTATGAATCAAAA+CGG | - | Chr3:63878714-63878733 | None:intergenic | 20.0% |
| !!! | TTTTTAAAGAGTTGATTCAT+AGG | + | Chr3:63879596-63879615 | MsG0380015008.01.T01:intron | 20.0% |
| !!! | TTTTTTATCAGCTTATTAGA+TGG | - | Chr3:63880168-63880187 | None:intergenic | 20.0% |
| ! | ATGCAATATAAAAAGAAGCA+TGG | + | Chr3:63879969-63879988 | MsG0380015008.01.T01:CDS | 25.0% |
| ! | CTAAACAATGAGTCAATATA+TGG | + | Chr3:63878526-63878545 | MsG0380015008.01.T01:exon | 25.0% |
| ! | TTGATGTAGATATACAACAT+TGG | + | Chr3:63879648-63879667 | MsG0380015008.01.T01:intron | 25.0% |
| ! | TTTCCATTATGAATCAAAAC+GGG | - | Chr3:63878713-63878732 | None:intergenic | 25.0% |
| !! | TATGTGTGTTATTATGTTCA+AGG | + | Chr3:63878881-63878900 | MsG0380015008.01.T01:intron | 25.0% |
| !!! | GTCTTGATTATTTTTTTGGT+TGG | + | Chr3:63879169-63879188 | MsG0380015008.01.T01:intron | 25.0% |
| !!! | TGACCTATTTTCTTTTTCAA+CGG | - | Chr3:63879352-63879371 | None:intergenic | 25.0% |
| AAAATTGCATTGGAAGAGAA+GGG | + | Chr3:63878605-63878624 | MsG0380015008.01.T01:CDS | 30.0% | |
| AATGAGAGTGAAAATTGCAT+TGG | + | Chr3:63878595-63878614 | MsG0380015008.01.T01:CDS | 30.0% | |
| ATGATCTATCGTTAAGAATG+AGG | - | Chr3:63879088-63879107 | None:intergenic | 30.0% | |
| ATTGTAAATTGTTTGCCACT+TGG | - | Chr3:63879504-63879523 | None:intergenic | 30.0% | |
| CTTCCGTTGAAAAAGAAAAT+AGG | + | Chr3:63879346-63879365 | MsG0380015008.01.T01:intron | 30.0% | |
| GAAAAAAATGTAGTTGAGAG+TGG | - | Chr3:63879285-63879304 | None:intergenic | 30.0% | |
| GATATACAACATTGGAAAGA+AGG | + | Chr3:63879656-63879675 | MsG0380015008.01.T01:intron | 30.0% | |
| GGTATCATGGTCTAATTTAT+TGG | + | Chr3:63879618-63879637 | MsG0380015008.01.T01:intron | 30.0% | |
| TAGTGATGTCTATAAAGCTT+TGG | - | Chr3:63878926-63878945 | None:intergenic | 30.0% | |
| TTCCATTATGAATCAAAACG+GGG | - | Chr3:63878712-63878731 | None:intergenic | 30.0% | |
| TTTACACTTACGAGACATAT+GGG | + | Chr3:63879832-63879851 | MsG0380015008.01.T01:CDS | 30.0% | |
| ! | CAAAACCATAGATTTTGTGA+GGG | - | Chr3:63879947-63879966 | None:intergenic | 30.0% |
| ! | CTTGGAGACAAAACATATTT+TGG | + | Chr3:63879756-63879775 | MsG0380015008.01.T01:CDS | 30.0% |
| ! | TTGTAAATTGTTTGCCACTT+GGG | - | Chr3:63879503-63879522 | None:intergenic | 30.0% |
| ! | TTTTACACTTACGAGACATA+TGG | + | Chr3:63879831-63879850 | MsG0380015008.01.T01:CDS | 30.0% |
| !!! | ATGTGGTTTTGTTGGATTTT+TGG | + | Chr3:63878558-63878577 | MsG0380015008.01.T01:CDS | 30.0% |
| AAATTGCATTGGAAGAGAAG+GGG | + | Chr3:63878606-63878625 | MsG0380015008.01.T01:CDS | 35.0% | |
| AATGAGTCAATATATGGCTG+AGG | + | Chr3:63878532-63878551 | MsG0380015008.01.T01:exon | 35.0% | |
| AATGTAATCTCCCCAAAACT+TGG | - | Chr3:63878834-63878853 | None:intergenic | 35.0% | |
| ACAACATTGGAAAGAAGGTA+TGG | + | Chr3:63879661-63879680 | MsG0380015008.01.T01:CDS | 35.0% | |
| AGTGTAAAACCAACTAGTGA+AGG | - | Chr3:63879820-63879839 | None:intergenic | 35.0% | |
| ATGAGTCAATATATGGCTGA+GGG | + | Chr3:63878533-63878552 | MsG0380015008.01.T01:CDS | 35.0% | |
| CAAAATTAGTCCGACAAAGA+TGG | - | Chr3:63879220-63879239 | None:intergenic | 35.0% | |
| GAAAATTGCATTGGAAGAGA+AGG | + | Chr3:63878604-63878623 | MsG0380015008.01.T01:CDS | 35.0% | |
| GACTTGAATAGAGATCGTTA+GGG | + | Chr3:63879991-63880010 | MsG0380015008.01.T01:exon | 35.0% | |
| GTGTAAAACCAACTAGTGAA+GGG | - | Chr3:63879819-63879838 | None:intergenic | 35.0% | |
| TATCGTTAAGAATGAGGTCT+AGG | - | Chr3:63879082-63879101 | None:intergenic | 35.0% | |
| TTGAGTACATTGATGAAGCT+TGG | + | Chr3:63878759-63878778 | MsG0380015008.01.T01:CDS | 35.0% | |
| ! | AGTTGATTCATAGGGTATCA+TGG | + | Chr3:63879605-63879624 | MsG0380015008.01.T01:intron | 35.0% |
| ! | CATCTTTGTCGGACTAATTT+TGG | + | Chr3:63879218-63879237 | MsG0380015008.01.T01:intron | 35.0% |
| ! | GCAAAACCATAGATTTTGTG+AGG | - | Chr3:63879948-63879967 | None:intergenic | 35.0% |
| !! | ATTTTGGTGGAGATGACTTT+GGG | + | Chr3:63879772-63879791 | MsG0380015008.01.T01:CDS | 35.0% |
| !! | GGAGACAAAACATATTTTGG+TGG | + | Chr3:63879759-63879778 | MsG0380015008.01.T01:CDS | 35.0% |
| !!! | ATCCCCGTTTTGATTCATAA+TGG | + | Chr3:63878707-63878726 | MsG0380015008.01.T01:CDS | 35.0% |
| !!! | TATTTTGGTGGAGATGACTT+TGG | + | Chr3:63879771-63879790 | MsG0380015008.01.T01:CDS | 35.0% |
| !!! | TTTTTGGTTGGTCACGTTAT+TGG | + | Chr3:63879181-63879200 | MsG0380015008.01.T01:intron | 35.0% |
| AACTGGAAACCCACTAAAAG+TGG | - | Chr3:63879261-63879280 | None:intergenic | 40.0% | |
| AAGGGTGAAGAACAAGAAGA+AGG | + | Chr3:63879690-63879709 | MsG0380015008.01.T01:CDS | 40.0% | |
| AATTAGTCCGACAAAGATGG+TGG | - | Chr3:63879217-63879236 | None:intergenic | 40.0% | |
| AATTGCATTGGAAGAGAAGG+GGG | + | Chr3:63878607-63878626 | MsG0380015008.01.T01:CDS | 40.0% | |
| ACTCTCATTCCATATGAGCT+TGG | - | Chr3:63878584-63878603 | None:intergenic | 40.0% | |
| ATGTAGTTGAGAGTGGAAAC+TGG | - | Chr3:63879278-63879297 | None:intergenic | 40.0% | |
| CTCTTATACCCTTCACTAGT+TGG | + | Chr3:63879808-63879827 | MsG0380015008.01.T01:CDS | 40.0% | |
| GAAAGAATGTCCTAAGCTTG+TGG | + | Chr3:63879869-63879888 | MsG0380015008.01.T01:CDS | 40.0% | |
| GAATGCTTGAAGACTCTAGA+AGG | + | Chr3:63879729-63879748 | MsG0380015008.01.T01:CDS | 40.0% | |
| GGACTTGAATAGAGATCGTT+AGG | + | Chr3:63879990-63880009 | MsG0380015008.01.T01:exon | 40.0% | |
| TATATCCATTCAAGCCCAAG+TGG | + | Chr3:63879486-63879505 | MsG0380015008.01.T01:intron | 40.0% | |
| TGACTTTGGGTATGTTGATG+TGG | + | Chr3:63879785-63879804 | MsG0380015008.01.T01:CDS | 40.0% | |
| ! | AAAGAAGGTATGGACAGGAA+AGG | + | Chr3:63879671-63879690 | MsG0380015008.01.T01:CDS | 40.0% |
| ! | AAGAAGGTATGGACAGGAAA+GGG | + | Chr3:63879672-63879691 | MsG0380015008.01.T01:CDS | 40.0% |
| ! | ACGGGGATCATTTTGTAAAC+CGG | - | Chr3:63878695-63878714 | None:intergenic | 40.0% |
| ! | AGGGCAAAAGAGAAGGTTTT+TGG | - | Chr3:63878787-63878806 | None:intergenic | 40.0% |
| ! | ATTGGAAAGAAGGTATGGAC+AGG | + | Chr3:63879666-63879685 | MsG0380015008.01.T01:CDS | 40.0% |
| ! | CTCCTTTTCCATACACCTTT+TGG | - | Chr3:63879901-63879920 | None:intergenic | 40.0% |
| ! | GAAGTATCCACCATCTTTGT+CGG | + | Chr3:63879207-63879226 | MsG0380015008.01.T01:intron | 40.0% |
| !! | CTAATTTTGGCCGGAGATTA+TGG | + | Chr3:63879231-63879250 | MsG0380015008.01.T01:intron | 40.0% |
| !! | TCTGCTTTTAGAGATGAACC+CGG | + | Chr3:63878673-63878692 | MsG0380015008.01.T01:CDS | 40.0% |
| !! | TTTGTCGGACTAATTTTGGC+CGG | + | Chr3:63879222-63879241 | MsG0380015008.01.T01:intron | 40.0% |
| !!! | GATTTTTGGCCAAGCTCATA+TGG | + | Chr3:63878572-63878591 | MsG0380015008.01.T01:CDS | 40.0% |
| !!! | TATGGCTAGCCACTTTTAGT+GGG | + | Chr3:63879249-63879268 | MsG0380015008.01.T01:intron | 40.0% |
| !!! | TTATGGCTAGCCACTTTTAG+TGG | + | Chr3:63879248-63879267 | MsG0380015008.01.T01:intron | 40.0% |
| AATGTCCTAAGCTTGTGGCT+TGG | + | Chr3:63879874-63879893 | MsG0380015008.01.T01:CDS | 45.0% | |
| AGAGATCGTTAGGGATTGCA+TGG | + | Chr3:63880000-63880019 | MsG0380015008.01.T01:exon | 45.0% | |
| ATATATGGCTGAGGGCAATG+TGG | + | Chr3:63878541-63878560 | MsG0380015008.01.T01:CDS | 45.0% | |
| ATGTCCTAAGCTTGTGGCTT+GGG | + | Chr3:63879875-63879894 | MsG0380015008.01.T01:CDS | 45.0% | |
| CCTCACCCTCACAAAATCTA+TGG | + | Chr3:63879939-63879958 | MsG0380015008.01.T01:CDS | 45.0% | |
| GAGATCGTTAGGGATTGCAT+GGG | + | Chr3:63880001-63880020 | MsG0380015008.01.T01:exon | 45.0% | |
| GTGTATGGAAAAGGAGAGTG+TGG | + | Chr3:63879905-63879924 | MsG0380015008.01.T01:CDS | 45.0% | |
| ! | AAGACTCTAGAAGGTGAGCT+TGG | + | Chr3:63879738-63879757 | MsG0380015008.01.T01:CDS | 45.0% |
| ! | AAGTGGCTAGCCATAATCTC+CGG | - | Chr3:63879244-63879263 | None:intergenic | 45.0% |
| ! | AGCGATCACAAGCCAAGTTT+TGG | + | Chr3:63878819-63878838 | MsG0380015008.01.T01:CDS | 45.0% |
| ! | CCATAGATTTTGTGAGGGTG+AGG | - | Chr3:63879942-63879961 | None:intergenic | 45.0% |
| ! | CGATCACAAGCCAAGTTTTG+GGG | + | Chr3:63878821-63878840 | MsG0380015008.01.T01:CDS | 45.0% |
| ! | CGGGGATCATTTTGTAAACC+GGG | - | Chr3:63878694-63878713 | None:intergenic | 45.0% |
| ! | GCGATCACAAGCCAAGTTTT+GGG | + | Chr3:63878820-63878839 | MsG0380015008.01.T01:CDS | 45.0% |
| ! | GGCCAAAAGGTGTATGGAAA+AGG | + | Chr3:63879896-63879915 | MsG0380015008.01.T01:CDS | 45.0% |
| ! | TGGCTTGTGATCGCTTGTAA+GGG | - | Chr3:63878814-63878833 | None:intergenic | 45.0% |
| !! | TTGGCTTGTGATCGCTTGTA+AGG | - | Chr3:63878815-63878834 | None:intergenic | 45.0% |
| !!! | TGAGGGCAATGTGGTTTTGT+TGG | + | Chr3:63878550-63878569 | MsG0380015008.01.T01:CDS | 45.0% |
| CTGCACACGAAGTTCTTCTC+AGG | - | Chr3:63880029-63880048 | None:intergenic | 50.0% | |
| GATCGCTTGTAAGGGTCAGA+GGG | - | Chr3:63878806-63878825 | None:intergenic | 50.0% | |
| TGATCGCTTGTAAGGGTCAG+AGG | - | Chr3:63878807-63878826 | None:intergenic | 50.0% | |
| ! | GTTTGCCACTTGGGCTTGAA+TGG | - | Chr3:63879494-63879513 | None:intergenic | 50.0% |
| GGGTCAGAGGGCAAAAGAGA+AGG | - | Chr3:63878794-63878813 | None:intergenic | 55.0% | |
| GTGAGGGTGAGGAAGTGACT+TGG | - | Chr3:63879931-63879950 | None:intergenic | 55.0% | |
| TTGGCCCAAGCCACAAGCTT+AGG | - | Chr3:63879882-63879901 | None:intergenic | 55.0% | |
| ! | AGCTTGTGGCTTGGGCCAAA+AGG | + | Chr3:63879883-63879902 | MsG0380015008.01.T01:CDS | 55.0% |
| ! | GGCTTGGGCCAAAAGGTGTA+TGG | + | Chr3:63879890-63879909 | MsG0380015008.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 63878474 | 63880238 | 63878474 | ID=MsG0380015008.01;Name=MsG0380015008.01 |
| Chr3 | mRNA | 63878474 | 63880238 | 63878474 | ID=MsG0380015008.01.T01;Parent=MsG0380015008.01;Name=MsG0380015008.01.T01;_AED=0.46;_eAED=0.46;_QI=59|1|1|1|1|1|2|237|224 |
| Chr3 | exon | 63878474 | 63878862 | 63878474 | ID=MsG0380015008.01.T01:exon:29783;Parent=MsG0380015008.01.T01 |
| Chr3 | exon | 63879657 | 63880238 | 63879657 | ID=MsG0380015008.01.T01:exon:29784;Parent=MsG0380015008.01.T01 |
| Chr3 | five_prime_UTR | 63878474 | 63878532 | 63878474 | ID=MsG0380015008.01.T01:five_prime_utr;Parent=MsG0380015008.01.T01 |
| Chr3 | CDS | 63878533 | 63878862 | 63878533 | ID=MsG0380015008.01.T01:cds;Parent=MsG0380015008.01.T01 |
| Chr3 | CDS | 63879657 | 63880001 | 63879657 | ID=MsG0380015008.01.T01:cds;Parent=MsG0380015008.01.T01 |
| Chr3 | three_prime_UTR | 63880002 | 63880238 | 63880002 | ID=MsG0380015008.01.T01:three_prime_utr;Parent=MsG0380015008.01.T01 |
| Gene Sequence |
| Protein sequence |
