AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022288.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022288.01.T01 MTR_4g087920 91.506 259 18 1 1 255 1 259 3.24e-164 456
MsG0480022288.01.T01 MTR_2g015890 55.118 254 102 4 19 268 11 256 1.03e-82 249
MsG0480022288.01.T01 MTR_2g015890 47.244 254 82 5 19 268 11 216 4.00e-59 187
MsG0480022288.01.T01 MTR_2g015890 58.182 165 62 2 104 268 1 158 5.15e-59 185
MsG0480022288.01.T01 MTR_2g091190 46.190 210 108 4 30 238 21 226 3.87e-46 155
MsG0480022288.01.T01 MTR_4g079760 38.996 259 149 7 1 258 1 251 8.09e-41 141
MsG0480022288.01.T01 MTR_2g015890 46.061 165 42 3 104 268 1 118 1.43e-35 124
MsG0480022288.01.T01 MTR_3g070970 34.673 199 110 7 65 253 67 255 1.27e-28 110
MsG0480022288.01.T01 MTR_1g093750 41.667 180 94 5 57 232 144 316 3.65e-25 102
MsG0480022288.01.T01 MTR_5g032375 31.737 167 97 4 69 223 43 204 2.03e-15 73.6
MsG0480022288.01.T01 MTR_5g066080 33.000 200 91 4 64 230 30 219 1.66e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022288.01.T01 AT3G56770 39.205 176 98 5 63 236 37 205 7.08e-30 112
MsG0480022288.01.T01 AT2G40200 41.667 132 73 2 73 201 65 195 2.46e-27 106
MsG0480022288.01.T01 AT1G68810 40.306 196 111 5 57 251 160 350 4.89e-24 99.8
MsG0480022288.01.T01 AT2G41130 38.068 176 101 4 63 236 59 228 5.22e-24 97.8
MsG0480022288.01.T01 AT3G25710 32.642 193 110 4 27 202 72 261 5.73e-21 90.9

Find 52 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATTCTTCTCCTCTTATT+TGG 0.100613 4:+70696388 None:intergenic
ACCACCACATTCATTGGTTT+TGG 0.275049 4:-70699465 MsG0480022288.01.T01:CDS
GAACCATATGATACTAAATT+AGG 0.319188 4:-70696694 MsG0480022288.01.T01:CDS
GTCATCACCACCACATTCAT+TGG 0.344688 4:-70699471 MsG0480022288.01.T01:CDS
AGAAGAAGGGAGAGAATAAA+TGG 0.348920 4:-70699329 MsG0480022288.01.T01:CDS
TATGATACTAAATTAGGATA+TGG 0.351083 4:-70696688 MsG0480022288.01.T01:CDS
AATCAAACTTTGGATAACTT+TGG 0.378387 4:-70699509 MsG0480022288.01.T01:CDS
TTCACTTCATCAACTTCTGT+TGG 0.395317 4:+70696721 None:intergenic
TATCCTAATTTAGTATCATA+TGG 0.402521 4:+70696691 None:intergenic
TTAAGGTCACATAGTATCTC+AGG 0.426548 4:+70696625 None:intergenic
GAACTTCATATCCAAATAAG+AGG 0.441039 4:-70696399 MsG0480022288.01.T01:CDS
GGGAAGCAGAGAGGAGAAGA+AGG 0.448212 4:-70699343 MsG0480022288.01.T01:CDS
TTCTCCTCTCTGCTTCCCTA+TGG 0.451668 4:+70699348 None:intergenic
ACTGGATTCTGTGCTGGAAA+AGG 0.471850 4:-70696449 MsG0480022288.01.T01:CDS
CAAGGCACTGGATTCTGTGC+TGG 0.480944 4:-70696455 MsG0480022288.01.T01:CDS
TAGCTTCTTTGAAGAACCAT+AGG 0.484232 4:-70699364 MsG0480022288.01.T01:CDS
GAGTTTGTTCAATCAAACTT+TGG 0.484332 4:-70699519 MsG0480022288.01.T01:CDS
GGAAGCAGAGAGGAGAAGAA+GGG 0.491306 4:-70699342 MsG0480022288.01.T01:CDS
GAATGAGAAGGAAAAGGCGG+TGG 0.493115 4:-70699441 MsG0480022288.01.T01:CDS
TGTAGCTTCCACTCAACAAA+AGG 0.500104 4:-70699282 MsG0480022288.01.T01:intron
GGAAAAGGCTTCTAACTCAA+TGG 0.501654 4:-70696434 MsG0480022288.01.T01:CDS
TTGTCTTTACATGTTGCAAA+GGG 0.509752 4:-70696525 MsG0480022288.01.T01:CDS
AAATGTCAACATTGGAAAGC+AGG 0.511259 4:-70696561 MsG0480022288.01.T01:CDS
CATGCATCTTGTTCATGCTA+AGG 0.515195 4:-70696334 None:intergenic
GATAACTTTGGAGCTTCCAA+TGG 0.517984 4:-70699497 MsG0480022288.01.T01:CDS
TTTGTCTTTACATGTTGCAA+AGG 0.523070 4:-70696526 MsG0480022288.01.T01:CDS
AAATGTTGTTCACAAGGCAC+TGG 0.530660 4:-70696467 MsG0480022288.01.T01:CDS
TTTGGAGAATGAGAAGGAAA+AGG 0.532337 4:-70699447 MsG0480022288.01.T01:CDS
GATGATGTTTCTGAGGTGAA+AGG 0.532394 4:-70699389 MsG0480022288.01.T01:CDS
GAAGGCAGAAATGTCAACAT+TGG 0.560990 4:-70696569 MsG0480022288.01.T01:CDS
ATGCATCTTGTTCATGCTAA+GGG 0.564588 4:-70696333 None:intergenic
GAGAGAATAAATGGTCACCT+TGG 0.565872 4:-70699320 MsG0480022288.01.T01:CDS
GGAGTGCCCTGTTACTGAAA+AGG 0.568614 4:-70699420 MsG0480022288.01.T01:CDS
GGAATTAAAGAAGAATGCAA+TGG 0.577626 4:-70696767 MsG0480022288.01.T01:CDS
AGCTTCTTTGAAGAACCATA+GGG 0.592888 4:-70699363 MsG0480022288.01.T01:CDS
AGATGCATGGCTGCATGAAG+AGG 0.593504 4:+70696348 None:intergenic
AATCACTTCAGCAAGTATTG+TGG 0.602708 4:+70696801 None:intergenic
TGCCCTGTTACTGAAAAGGT+TGG 0.605529 4:-70699416 MsG0480022288.01.T01:CDS
TATTGCAAATGTTGTTCACA+AGG 0.609473 4:-70696473 MsG0480022288.01.T01:CDS
CAAGACCACGTAATGTTCCA+AGG 0.609560 4:+70699303 None:intergenic
TGAAGTGATTAGTCAAGTGA+AGG 0.620505 4:-70696788 MsG0480022288.01.T01:CDS
GGAGAATGAGAAGGAAAAGG+CGG 0.620734 4:-70699444 MsG0480022288.01.T01:CDS
TTCTCCAAAACCAATGAATG+TGG 0.622319 4:+70699461 None:intergenic
TGGTGGTGATGACAATCCAT+TGG 0.624935 4:+70699481 None:intergenic
CTTAGCATGAACAAGATGCA+TGG 0.629347 4:+70696335 None:intergenic
ACTTCAACTTCAACTAGTGA+AGG 0.632952 4:-70696587 MsG0480022288.01.T01:CDS
AATGCAATGGAAGCAAGCAA+AGG 0.642370 4:-70696754 MsG0480022288.01.T01:CDS
TGGTAAAGATGATGTTTCTG+AGG 0.646156 4:-70699396 MsG0480022288.01.T01:CDS
GGTCACATAGTATCTCAGGT+TGG 0.655590 4:+70696629 None:intergenic
TCCAAAACCAATGAATGTGG+TGG 0.695002 4:+70699464 None:intergenic
GGTCACCTTGGAACATTACG+TGG 0.700048 4:-70699308 MsG0480022288.01.T01:CDS
AGAACCATAGGGAAGCAGAG+AGG 0.739044 4:-70699352 MsG0480022288.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG - Chr4:70697041-70697060 MsG0480022288.01.T01:intron 10.0%
!! CATTATTGTAATTAAATTAA+GGG - Chr4:70697803-70697822 MsG0480022288.01.T01:intron 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - Chr4:70697040-70697059 MsG0480022288.01.T01:intron 10.0%
!!! AATTATATATTTTTTTTGGA+AGG + Chr4:70699004-70699023 None:intergenic 10.0%
!!! ATTATATATTTTTTTTGGAA+GGG + Chr4:70699003-70699022 None:intergenic 10.0%
!!! TAAAGTATTTTTTATTCTAA+AGG + Chr4:70697621-70697640 None:intergenic 10.0%
!!! TGATAATTTTAAAAGAAAAA+TGG - Chr4:70697726-70697745 MsG0480022288.01.T01:intron 10.0%
!! AAAAATAAACACAAACTAAA+AGG + Chr4:70697456-70697475 None:intergenic 15.0%
!! ACTCCTATATTAATATTATA+AGG - Chr4:70698470-70698489 MsG0480022288.01.T01:intron 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - Chr4:70697042-70697061 MsG0480022288.01.T01:intron 15.0%
!! ATTATTATTATCCAAATCTA+TGG - Chr4:70696864-70696883 MsG0480022288.01.T01:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - Chr4:70697106-70697125 MsG0480022288.01.T01:intron 15.0%
!! CTCCTATATTAATATTATAA+GGG - Chr4:70698471-70698490 MsG0480022288.01.T01:intron 15.0%
!! GACTAAATTAAGTTTAAATA+TGG - Chr4:70698775-70698794 MsG0480022288.01.T01:intron 15.0%
!! GCATTATTGTAATTAAATTA+AGG - Chr4:70697802-70697821 MsG0480022288.01.T01:intron 15.0%
!! TAAATAAGCATTAAGAAATA+AGG + Chr4:70697675-70697694 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - Chr4:70697105-70697124 MsG0480022288.01.T01:intron 15.0%
!! TTATTATTATCCAAATCTAT+GGG - Chr4:70696865-70696884 MsG0480022288.01.T01:intron 15.0%
!! TTCCCTTATAATATTAATAT+AGG + Chr4:70698476-70698495 None:intergenic 15.0%
!!! TCATGATATTAGTATTTTTA+TGG - Chr4:70697960-70697979 MsG0480022288.01.T01:intron 15.0%
!!! TTATATATTTTTTTTGGAAG+GGG + Chr4:70699002-70699021 None:intergenic 15.0%
!!! TTCTAATTGTAAAATTTGTT+TGG - Chr4:70696705-70696724 MsG0480022288.01.T01:CDS 15.0%
!! AGATAACACATATATGATAT+CGG - Chr4:70697519-70697538 MsG0480022288.01.T01:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG - Chr4:70697138-70697157 MsG0480022288.01.T01:intron 20.0%
!! ATGAAGGATGATTATAATAT+GGG + Chr4:70698666-70698685 None:intergenic 20.0%
!! ATTCAATATCAAAATTCTGA+TGG + Chr4:70697404-70697423 None:intergenic 20.0%
!! ATTTAATGGAAAGTTGAAAT+TGG - Chr4:70697227-70697246 MsG0480022288.01.T01:intron 20.0%
!! CAAATATTATTTCCTTGATT+AGG - Chr4:70698049-70698068 MsG0480022288.01.T01:intron 20.0%
!! TATCCTAATTTAGTATCATA+TGG + Chr4:70699217-70699236 None:intergenic 20.0%
!! TATGATACTAAATTAGGATA+TGG - Chr4:70699217-70699236 MsG0480022288.01.T01:intron 20.0%
!! TTTAATGGAAAGTTGAAATT+GGG - Chr4:70697228-70697247 MsG0480022288.01.T01:intron 20.0%
!!! ATAGTATAGTTTTGGTTTTT+AGG - Chr4:70697263-70697282 MsG0480022288.01.T01:intron 20.0%
!!! GAACAAATATAGTATAGTTT+TGG - Chr4:70697255-70697274 MsG0480022288.01.T01:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - Chr4:70697174-70697193 MsG0480022288.01.T01:intron 20.0%
!!! TTGTTGTCTTATTTCTATAA+TGG - Chr4:70698340-70698359 MsG0480022288.01.T01:intron 20.0%
!!! TTTTTTACGGTAATAATACA+TGG - Chr4:70698848-70698867 MsG0480022288.01.T01:intron 20.0%
! AACAAATAAAGATAAGATGG+CGG + Chr4:70698325-70698344 None:intergenic 25.0%
! AACACAAACTAAAAGGATAT+TGG + Chr4:70697449-70697468 None:intergenic 25.0%
! AAGAAATAAGGAAATGCTAA+GGG + Chr4:70697663-70697682 None:intergenic 25.0%
! AATCAAACTTTGGATAACTT+TGG - Chr4:70696396-70696415 MsG0480022288.01.T01:CDS 25.0%
! ACTTCAAATTTCAAAACTTG+TGG + Chr4:70697782-70697801 None:intergenic 25.0%
! AGGTGAGAATCAAATATAAT+TGG - Chr4:70697335-70697354 MsG0480022288.01.T01:intron 25.0%
! ATATTGCATAAAACATACGT+AGG - Chr4:70696768-70696787 MsG0480022288.01.T01:CDS 25.0%
! ATTCAAGACAATGTTATCAA+AGG - Chr4:70698738-70698757 MsG0480022288.01.T01:intron 25.0%
! GAACCATATGATACTAAATT+AGG - Chr4:70699211-70699230 MsG0480022288.01.T01:intron 25.0%
! GACAACAAATAAAGATAAGA+TGG + Chr4:70698328-70698347 None:intergenic 25.0%
! GATGAAGGATGATTATAATA+TGG + Chr4:70698667-70698686 None:intergenic 25.0%
! GGTGAGAATCAAATATAATT+GGG - Chr4:70697336-70697355 MsG0480022288.01.T01:intron 25.0%
! TAAGAAATAAGGAAATGCTA+AGG + Chr4:70697664-70697683 None:intergenic 25.0%
! TAATAAATCAACTCAGTTGT+TGG - Chr4:70698692-70698711 MsG0480022288.01.T01:intron 25.0%
! TAATGAATGATTTCCAGTTT+CGG + Chr4:70698002-70698021 None:intergenic 25.0%
!! AGTTTAAGTTTGAACAAAGA+TGG - Chr4:70697292-70697311 MsG0480022288.01.T01:intron 25.0%
!! ATATACTTTTGTTCATGTCA+TGG + Chr4:70698078-70698097 None:intergenic 25.0%
!! ATGGTTCATTACATTTTAGA+TGG - Chr4:70699075-70699094 MsG0480022288.01.T01:intron 25.0%
!! TATTTCTATAATGGTGATGT+GGG - Chr4:70698349-70698368 MsG0480022288.01.T01:intron 25.0%
!! TTTTATGGTTTCTTCTTCTA+TGG - Chr4:70697975-70697994 MsG0480022288.01.T01:intron 25.0%
!!! ACAACTGAGTTGATTTATTA+AGG + Chr4:70698692-70698711 None:intergenic 25.0%
!!! ATATTAATATAGGAGTACTG+AGG + Chr4:70698466-70698485 None:intergenic 25.0%
!!! ATGTGTTGAATTTGATTTTG+TGG - Chr4:70699048-70699067 MsG0480022288.01.T01:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - Chr4:70697173-70697192 MsG0480022288.01.T01:intron 25.0%
!!! GTGCATTAATTGCTTTTTTA+AGG + Chr4:70699300-70699319 None:intergenic 25.0%
!!! TGTGTTGAATTTGATTTTGT+GGG - Chr4:70699049-70699068 MsG0480022288.01.T01:intron 25.0%
!!! TTATTTCTATAATGGTGATG+TGG - Chr4:70698348-70698367 MsG0480022288.01.T01:intron 25.0%
AAATATAATTGGGTACCTGA+GGG - Chr4:70697346-70697365 MsG0480022288.01.T01:intron 30.0%
ACACTTCACAATCAAATTGA+AGG - Chr4:70696803-70696822 MsG0480022288.01.T01:CDS 30.0%
ACTAATCACGTTCAAATATG+TGG - Chr4:70698904-70698923 MsG0480022288.01.T01:intron 30.0%
ATTGAAATGGCTCAGTTATT+TGG - Chr4:70697151-70697170 MsG0480022288.01.T01:intron 30.0%
CAAAAAGAACAATGAAAGCA+AGG + Chr4:70696665-70696684 None:intergenic 30.0%
GAACTTCATATCCAAATAAG+AGG - Chr4:70699506-70699525 MsG0480022288.01.T01:CDS 30.0%
GAAGGGAGTAATAGATTTAA+TGG - Chr4:70697213-70697232 MsG0480022288.01.T01:intron 30.0%
GGAATTAAAGAAGAATGCAA+TGG - Chr4:70699138-70699157 MsG0480022288.01.T01:intron 30.0%
GTGCAATTATATATCCCATA+TGG - Chr4:70697923-70697942 MsG0480022288.01.T01:intron 30.0%
TATTGCAAATGTTGTTCACA+AGG - Chr4:70699432-70699451 MsG0480022288.01.T01:CDS 30.0%
TCAATTTGATTGTGAAGTGT+CGG + Chr4:70696803-70696822 None:intergenic 30.0%
TGATTTATTAAGGTGGATGA+AGG + Chr4:70698682-70698701 None:intergenic 30.0%
TTGAAATGGCTCAGTTATTT+GGG - Chr4:70697152-70697171 MsG0480022288.01.T01:intron 30.0%
TTGTCTTTACATGTTGCAAA+GGG - Chr4:70699380-70699399 MsG0480022288.01.T01:CDS 30.0%
TTTGTCTTTACATGTTGCAA+AGG - Chr4:70699379-70699398 MsG0480022288.01.T01:CDS 30.0%
! AAATTATCAAAGAGCGTGAA+AGG + Chr4:70697715-70697734 None:intergenic 30.0%
! AATTATCAAAGAGCGTGAAA+GGG + Chr4:70697714-70697733 None:intergenic 30.0%
! ATAATATGGGTTGTGTTAAC+CGG + Chr4:70698653-70698672 None:intergenic 30.0%
! ATTTCTATAATGGTGATGTG+GGG - Chr4:70698350-70698369 MsG0480022288.01.T01:intron 30.0%
! GAGTTTGTTCAATCAAACTT+TGG - Chr4:70696386-70696405 MsG0480022288.01.T01:CDS 30.0%
! TACTGATTGCTAAAGTCAAA+TGG - Chr4:70697876-70697895 MsG0480022288.01.T01:intron 30.0%
! TATTGACAATATTTTCCCTC+AGG + Chr4:70697364-70697383 None:intergenic 30.0%
!! AATTTGATTTTGTGGGTCAA+TGG - Chr4:70699056-70699075 MsG0480022288.01.T01:intron 30.0%
!! ACACTAATGTCAATTTTGAC+AGG - Chr4:70698108-70698127 MsG0480022288.01.T01:intron 30.0%
!! ACTGAGTTGATTTATTAAGG+TGG + Chr4:70698689-70698708 None:intergenic 30.0%
!! CACTAATGTCAATTTTGACA+GGG - Chr4:70698109-70698128 MsG0480022288.01.T01:intron 30.0%
!!! AGGGAATAGTTTTAATGTTG+AGG - Chr4:70699399-70699418 MsG0480022288.01.T01:CDS 30.0%
!!! ATTGCTTTCAAGTTTTGTTG+TGG - Chr4:70698212-70698231 MsG0480022288.01.T01:intron 30.0%
AAATGTCAACATTGGAAAGC+AGG - Chr4:70699344-70699363 MsG0480022288.01.T01:CDS 35.0%
AAGAACAATGAAAGCAAGGA+AGG + Chr4:70696661-70696680 None:intergenic 35.0%
AATATGTGTGAAATGAGCCA+AGG + Chr4:70697021-70697040 None:intergenic 35.0%
ACGATATTGACCCATAGATT+TGG + Chr4:70696878-70696897 None:intergenic 35.0%
ACTTCAACTTCAACTAGTGA+AGG - Chr4:70699318-70699337 MsG0480022288.01.T01:CDS 35.0%
AGAAAATGGGTCAGATATTC+CGG - Chr4:70697187-70697206 MsG0480022288.01.T01:intron 35.0%
AGAAGAAGGGAGAGAATAAA+TGG - Chr4:70696576-70696595 MsG0480022288.01.T01:CDS 35.0%
AGATGGCAATACAAGCTTTA+AGG - Chr4:70697309-70697328 MsG0480022288.01.T01:intron 35.0%
ATATGTGTGAAATGAGCCAA+GGG + Chr4:70697020-70697039 None:intergenic 35.0%
CAAATATAATTGGGTACCTG+AGG - Chr4:70697345-70697364 MsG0480022288.01.T01:intron 35.0%
CACATTCTTCTCCTCTTATT+TGG + Chr4:70699520-70699539 None:intergenic 35.0%
CTTCACAATCAAATTGAAGG+TGG - Chr4:70696806-70696825 MsG0480022288.01.T01:CDS 35.0%
GCATCATGGTTGTCATTATA+TGG - Chr4:70697835-70697854 MsG0480022288.01.T01:intron 35.0%
TCTATAAAATGCATGTGCTG+TGG - Chr4:70696951-70696970 MsG0480022288.01.T01:intron 35.0%
TCTTTACAAGTCTCGAGAAT+TGG + Chr4:70697580-70697599 None:intergenic 35.0%
TTAAGGTCACATAGTATCTC+AGG + Chr4:70699283-70699302 None:intergenic 35.0%
TTCACTTCATCAACTTCTGT+TGG + Chr4:70699187-70699206 None:intergenic 35.0%
TTCTCCAAAACCAATGAATG+TGG + Chr4:70696447-70696466 None:intergenic 35.0%
TTTGGAAGGGGAAAAATTGT+TGG + Chr4:70698990-70699009 None:intergenic 35.0%
TTTGGAGAATGAGAAGGAAA+AGG - Chr4:70696458-70696477 MsG0480022288.01.T01:CDS 35.0%
! AACTCAGTTTGTTTGCCATA+TGG + Chr4:70697941-70697960 None:intergenic 35.0%
! AAGACATACCTTTTGTTGAG+TGG + Chr4:70696634-70696653 None:intergenic 35.0%
! AATCACTTCAGCAAGTATTG+TGG + Chr4:70699107-70699126 None:intergenic 35.0%
! ACTCAGTTTGTTTGCCATAT+GGG + Chr4:70697940-70697959 None:intergenic 35.0%
! AGCTTCTTTGAAGAACCATA+GGG - Chr4:70696542-70696561 MsG0480022288.01.T01:CDS 35.0%
! CGGGTTAAGGTATCTAAAAA+AGG + Chr4:70698617-70698636 None:intergenic 35.0%
! TACCAACCTTTTCAGTAACA+GGG + Chr4:70696494-70696513 None:intergenic 35.0%
! TAGCTTCTTTGAAGAACCAT+AGG - Chr4:70696541-70696560 MsG0480022288.01.T01:CDS 35.0%
! TGAAGTGATTAGTCAAGTGA+AGG - Chr4:70699117-70699136 MsG0480022288.01.T01:intron 35.0%
! TGGTAAAGATGATGTTTCTG+AGG - Chr4:70696509-70696528 MsG0480022288.01.T01:CDS 35.0%
! TGGTAACCATGTCTCTTTTA+GGG - Chr4:70696901-70696920 MsG0480022288.01.T01:intron 35.0%
! TTACCAACCTTTTCAGTAAC+AGG + Chr4:70696495-70696514 None:intergenic 35.0%
!!! ATTGGTTTTGGAGAATGAGA+AGG - Chr4:70696452-70696471 MsG0480022288.01.T01:CDS 35.0%
!!! GACAGGGTATTTTAACTTGA+TGG - Chr4:70698125-70698144 MsG0480022288.01.T01:intron 35.0%
AAATGTTGTTCACAAGGCAC+TGG - Chr4:70699438-70699457 MsG0480022288.01.T01:CDS 40.0%
AAGGGATTCTGATTGCATCA+TGG - Chr4:70697821-70697840 MsG0480022288.01.T01:intron 40.0%
AATGCAATGGAAGCAAGCAA+AGG - Chr4:70699151-70699170 MsG0480022288.01.T01:intron 40.0%
ATCAAGCCCTAAAAGAGACA+TGG + Chr4:70696910-70696929 None:intergenic 40.0%
ATGTCATGGAGACCTAATCA+AGG + Chr4:70698064-70698083 None:intergenic 40.0%
GAAAATGGGTCAGATATTCC+GGG - Chr4:70697188-70697207 MsG0480022288.01.T01:intron 40.0%
GAAGGCAGAAATGTCAACAT+TGG - Chr4:70699336-70699355 MsG0480022288.01.T01:CDS 40.0%
GAGAGAATAAATGGTCACCT+TGG - Chr4:70696585-70696604 MsG0480022288.01.T01:CDS 40.0%
GGAAAAGGCTTCTAACTCAA+TGG - Chr4:70699471-70699490 MsG0480022288.01.T01:CDS 40.0%
GGAAAGAGGAAGACAACAAA+TGG + Chr4:70698445-70698464 None:intergenic 40.0%
TCCAAAACCAATGAATGTGG+TGG + Chr4:70696444-70696463 None:intergenic 40.0%
TGGAAAGTTGAAATTGGGTG+AGG - Chr4:70697233-70697252 MsG0480022288.01.T01:intron 40.0%
TGTAGCTTCCACTCAACAAA+AGG - Chr4:70696623-70696642 MsG0480022288.01.T01:CDS 40.0%
! CTGGTAACCATGTCTCTTTT+AGG - Chr4:70696900-70696919 MsG0480022288.01.T01:intron 40.0%
! GTGATTAGTCATGTGTCACA+CGG + Chr4:70698893-70698912 None:intergenic 40.0%
! TGATTAGTCATGTGTCACAC+GGG + Chr4:70698892-70698911 None:intergenic 40.0%
!! CAATACAAGCTTTAAGGCAG+AGG - Chr4:70697315-70697334 MsG0480022288.01.T01:intron 40.0%
!! GATAACTTTGGAGCTTCCAA+TGG - Chr4:70696408-70696427 MsG0480022288.01.T01:CDS 40.0%
!! GATGATGTTTCTGAGGTGAA+AGG - Chr4:70696516-70696535 MsG0480022288.01.T01:CDS 40.0%
!! TATAGGAGTACTGAGGAAAG+AGG + Chr4:70698459-70698478 None:intergenic 40.0%
!!! ACCACCACATTCATTGGTTT+TGG - Chr4:70696440-70696459 MsG0480022288.01.T01:CDS 40.0%
ACTGGATTCTGTGCTGGAAA+AGG - Chr4:70699456-70699475 MsG0480022288.01.T01:CDS 45.0%
AGCCAAGGGCTCAGATAAAT+TGG + Chr4:70697006-70697025 None:intergenic 45.0%
AGGGCTCAGATAAATTGGGA+CGG + Chr4:70697001-70697020 None:intergenic 45.0%
ATACCATCCAGAAGAACACC+TGG + Chr4:70698243-70698262 None:intergenic 45.0%
ATCTATTACTCCCTTCGTCC+CGG + Chr4:70697209-70697228 None:intergenic 45.0%
ATGTGCTGTGGAGATGAGAA+AGG - Chr4:70696963-70696982 MsG0480022288.01.T01:intron 45.0%
CAAGACCACGTAATGTTCCA+AGG + Chr4:70696605-70696624 None:intergenic 45.0%
CTATGGGTCAATATCGTGTC+TGG - Chr4:70696881-70696900 MsG0480022288.01.T01:intron 45.0%
CTCAGATAAATTGGGACGGA+GGG + Chr4:70696997-70697016 None:intergenic 45.0%
CTTCTTCTATGGTCCGAAAC+TGG - Chr4:70697986-70698005 MsG0480022288.01.T01:intron 45.0%
GCCAAGGGCTCAGATAAATT+GGG + Chr4:70697005-70697024 None:intergenic 45.0%
GGAGAATGAGAAGGAAAAGG+CGG - Chr4:70696461-70696480 MsG0480022288.01.T01:CDS 45.0%
GGTCACATAGTATCTCAGGT+TGG + Chr4:70699279-70699298 None:intergenic 45.0%
GTCATCACCACCACATTCAT+TGG - Chr4:70696434-70696453 MsG0480022288.01.T01:CDS 45.0%
TCCCAATTTATCTGAGCCCT+TGG - Chr4:70697001-70697020 MsG0480022288.01.T01:intron 45.0%
TGCCCTGTTACTGAAAAGGT+TGG - Chr4:70696489-70696508 MsG0480022288.01.T01:CDS 45.0%
TGTGCTGTGGAGATGAGAAA+GGG - Chr4:70696964-70696983 MsG0480022288.01.T01:intron 45.0%
! TGGTGGTGATGACAATCCAT+TGG + Chr4:70696427-70696446 None:intergenic 45.0%
!!! AGTTTTGTTGTGGACATGCC+AGG - Chr4:70698222-70698241 MsG0480022288.01.T01:intron 45.0%
!!! AAATTTAATCATTTTTTTTA+CGG - Chr4:70698835-70698854 MsG0480022288.01.T01:intron 5.0%
!!! AAGTAATTATATATTTTTTT+TGG + Chr4:70699008-70699027 None:intergenic 5.0%
!!! TAAAAAAAATGATTAAATTT+TGG + Chr4:70698835-70698854 None:intergenic 5.0%
AAATGTTAACCAGTGCCCCC+GGG - Chr4:70698494-70698513 MsG0480022288.01.T01:intron 50.0%
AGAACCATAGGGAAGCAGAG+AGG - Chr4:70696553-70696572 MsG0480022288.01.T01:CDS 50.0%
AGATGCATGGCTGCATGAAG+AGG + Chr4:70699560-70699579 None:intergenic 50.0%
ATGCCAGGTGTTCTTCTGGA+TGG - Chr4:70698237-70698256 MsG0480022288.01.T01:intron 50.0%
CACACGGGTGTACATAGAGT+GGG + Chr4:70698877-70698896 None:intergenic 50.0%
GAAATGTTAACCAGTGCCCC+CGG - Chr4:70698493-70698512 MsG0480022288.01.T01:intron 50.0%
GAATGAGAAGGAAAAGGCGG+TGG - Chr4:70696464-70696483 MsG0480022288.01.T01:CDS 50.0%
GCTCAGATAAATTGGGACGG+AGG + Chr4:70696998-70697017 None:intergenic 50.0%
GGAAGCAGAGAGGAGAAGAA+GGG - Chr4:70696563-70696582 MsG0480022288.01.T01:CDS 50.0%
GGTCACCTTGGAACATTACG+TGG - Chr4:70696597-70696616 MsG0480022288.01.T01:CDS 50.0%
GTCAGATATTCCGGGACGAA+GGG - Chr4:70697196-70697215 MsG0480022288.01.T01:intron 50.0%
TCACACGGGTGTACATAGAG+TGG + Chr4:70698878-70698897 None:intergenic 50.0%
TTCTCCTCTCTGCTTCCCTA+TGG + Chr4:70696560-70696579 None:intergenic 50.0%
! GGAGTGCCCTGTTACTGAAA+AGG - Chr4:70696485-70696504 MsG0480022288.01.T01:CDS 50.0%
AATGTTAACCAGTGCCCCCG+GGG - Chr4:70698495-70698514 MsG0480022288.01.T01:intron 55.0%
AGATACCTTAACCCGTGCCC+CGG - Chr4:70698622-70698641 MsG0480022288.01.T01:intron 55.0%
GGACATGCCAGGTGTTCTTC+TGG - Chr4:70698233-70698252 MsG0480022288.01.T01:intron 55.0%
GGGAAGCAGAGAGGAGAAGA+AGG - Chr4:70696562-70696581 MsG0480022288.01.T01:CDS 55.0%
GGTCAGATATTCCGGGACGA+AGG - Chr4:70697195-70697214 MsG0480022288.01.T01:intron 55.0%
TGCTCTCTTAACCAGTGCCC+CGG + Chr4:70698515-70698534 None:intergenic 55.0%
!! CAAGGCACTGGATTCTGTGC+TGG - Chr4:70699450-70699469 MsG0480022288.01.T01:CDS 55.0%
ATACCTTAACCCGTGCCCCG+GGG - Chr4:70698624-70698643 MsG0480022288.01.T01:intron 60.0%
CTCTCTTAACCAGTGCCCCG+GGG + Chr4:70698513-70698532 None:intergenic 60.0%
GATACCTTAACCCGTGCCCC+GGG - Chr4:70698623-70698642 MsG0480022288.01.T01:intron 60.0%
GCTCTCTTAACCAGTGCCCC+GGG + Chr4:70698514-70698533 None:intergenic 60.0%
GTTGTGTTAACCGGTGCCCC+CGG + Chr4:70698644-70698663 None:intergenic 60.0%
TCTCTTAACCAGTGCCCCGG+GGG + Chr4:70698512-70698531 None:intergenic 60.0%
TTGTGTTAACCGGTGCCCCC+GGG + Chr4:70698643-70698662 None:intergenic 60.0%
TACCTTAACCCGTGCCCCGG+GGG - Chr4:70698625-70698644 MsG0480022288.01.T01:intron 65.0%
TGTGTTAACCGGTGCCCCCG+GGG + Chr4:70698642-70698661 None:intergenic 65.0%
AACCAGTGCCCCCGGGGCAC+TGG - Chr4:70698501-70698520 MsG0480022288.01.T01:intron 75.0%
TAACCGGTGCCCCCGGGGCA+CGG + Chr4:70698637-70698656 None:intergenic 75.0%
! AACCAGTGCCCCGGGGGCAC+TGG + Chr4:70698506-70698525 None:intergenic 75.0%
! TGCCCCCGGGGCACGGGTTA+AGG + Chr4:70698630-70698649 None:intergenic 75.0%
! AACCGGTGCCCCCGGGGCAC+GGG + Chr4:70698636-70698655 None:intergenic 80.0%
!! AACCCGTGCCCCGGGGGCAC+CGG - Chr4:70698631-70698650 MsG0480022288.01.T01:intron 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70696336 70699591 70696336 ID=MsG0480022288.01;Name=MsG0480022288.01
Chr4 mRNA 70696336 70699591 70696336 ID=MsG0480022288.01.T01;Parent=MsG0480022288.01;Name=MsG0480022288.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|268
Chr4 exon 70699283 70699591 70699283 ID=MsG0480022288.01.T01:exon:19145;Parent=MsG0480022288.01.T01
Chr4 exon 70696336 70696833 70696336 ID=MsG0480022288.01.T01:exon:19146;Parent=MsG0480022288.01.T01
Chr4 CDS 70699283 70699591 70699283 ID=MsG0480022288.01.T01:cds;Parent=MsG0480022288.01.T01
Chr4 CDS 70696336 70696833 70696336 ID=MsG0480022288.01.T01:cds;Parent=MsG0480022288.01.T01
Gene Sequence

>MsG0480022288.01.T01

ATGGATTACTCATTTTATGACAAAATTTATTCATCAGCGTATGATCATTTGAGTTTGTTCAATCAAACTTTGGATAACTTTGGAGCTTCCAATGGATTGTCATCACCACCACATTCATTGGTTTTGGAGAATGAGAAGGAAAAGGCGGTGGAGTGCCCTGTTACTGAAAAGGTTGGTAAAGATGATGTTTCTGAGGTGAAAGGTTTAGCTTCTTTGAAGAACCATAGGGAAGCAGAGAGGAGAAGAAGGGAGAGAATAAATGGTCACCTTGGAACATTACGTGGTCTTGTAGCTTCCACTCAACAAAAGATGGATAAAGCCACAATACTTGCTGAAGTGATTAGTCAAGTGAAGGAATTAAAGAAGAATGCAATGGAAGCAAGCAAAGGTTTTCTCATTCCAACAGAAGTTGATGAAGTGAAAGTTGAACCATATGATACTAAATTAGGATATGGATGCATGTCTTACATAGCAACTATCTGCTGTGATTACCAACCTGAGATACTATGTGACCTTAAAAAAGCAATTAATGCACTTCAACTTCAACTAGTGAAGGCAGAAATGTCAACATTGGAAAGCAGGATGAAGAATATGTTTGTCTTTACATGTTGCAAAGGGAATAGTTTTAATGTTGAGGCATGTCAAAGTATTGCAAATGTTGTTCACAAGGCACTGGATTCTGTGCTGGAAAAGGCTTCTAACTCAATGGAATTCTCACTCAGAACTTCATATCCAAATAAGAGGAGAAGAATGTGCTTTGTTGAAACATCTACCTCTTCATGCAGCCATGCATCTTGTTCATGCTAA
Protein sequence

>MsG0480022288.01.T01

MDYSFYDKIYSSAYDHLSLFNQTLDNFGASNGLSSPPHSLVLENEKEKAVECPVTEKVGKDDVSEVKGLASLKNHREAERRRRERINGHLGTLRGLVASTQQKMDKATILAEVISQVKELKKNAMEASKGFLIPTEVDEVKVEPYDTKLGYGCMSYIATICCDYQPEILCDLKKAINALQLQLVKAEMSTLESRMKNMFVFTCCKGNSFNVEACQSIANVVHKALDSVLEKASNSMEFSLRTSYPNKRRRMCFVETSTSSCSHASCSC*