Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036472.01.T01 | XP_003621394.2 | 95.679 | 324 | 14 | 0 | 1 | 324 | 178 | 501 | 0 | 649 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0780036472.01 | 0.811256 | 7.665788e-51 | 4.722485e-48 |
| MsG0080048036.01 | MsG0780036472.01 | 0.800590 | 1.342045e-48 | 6.273035e-46 |
| MsG0080048134.01 | MsG0780036472.01 | 0.823026 | 1.728215e-53 | 1.470962e-50 |
| MsG0080048745.01 | MsG0780036472.01 | 0.803321 | 3.685955e-49 | 1.846660e-46 |
| MsG0080048992.01 | MsG0780036472.01 | -0.817306 | 3.530866e-52 | 2.562017e-49 |
| MsG0080049016.01 | MsG0780036472.01 | -0.812732 | 3.656728e-51 | 2.343124e-48 |
| MsG0180000147.01 | MsG0780036472.01 | 0.827972 | 1.164330e-54 | 1.141734e-51 |
| MsG0180000148.01 | MsG0780036472.01 | 0.819702 | 1.010917e-52 | 7.836933e-50 |
| MsG0180000287.01 | MsG0780036472.01 | 0.800620 | 1.323468e-48 | 6.190874e-46 |
| MsG0180000386.01 | MsG0780036472.01 | 0.804076 | 2.569314e-49 | 1.312537e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036472.01.T01 | MTR_7g012860 | 95.679 | 324 | 14 | 0 | 1 | 324 | 178 | 501 | 0.0 | 649 |
| MsG0780036472.01.T01 | MTR_0287s0060 | 95.679 | 324 | 14 | 0 | 1 | 324 | 178 | 501 | 0.0 | 649 |
| MsG0780036472.01.T01 | MTR_7g012330 | 87.654 | 324 | 38 | 1 | 1 | 324 | 179 | 500 | 0.0 | 598 |
| MsG0780036472.01.T01 | MTR_5g045770 | 63.889 | 324 | 115 | 2 | 2 | 324 | 175 | 497 | 1.06e-145 | 420 |
| MsG0780036472.01.T01 | MTR_4g026030 | 60.568 | 317 | 123 | 2 | 6 | 321 | 27 | 342 | 3.88e-144 | 410 |
| MsG0780036472.01.T01 | MTR_4g026050 | 62.154 | 325 | 121 | 2 | 1 | 324 | 174 | 497 | 1.35e-143 | 414 |
| MsG0780036472.01.T01 | MTR_4g025970 | 62.769 | 325 | 118 | 3 | 2 | 324 | 175 | 498 | 3.52e-143 | 414 |
| MsG0780036472.01.T01 | MTR_4g026200 | 59.306 | 317 | 127 | 2 | 6 | 321 | 179 | 494 | 6.02e-141 | 408 |
| MsG0780036472.01.T01 | MTR_4g025980 | 62.037 | 324 | 120 | 2 | 2 | 324 | 176 | 497 | 5.23e-139 | 403 |
| MsG0780036472.01.T01 | MTR_5g023680 | 60.127 | 316 | 124 | 2 | 7 | 321 | 156 | 470 | 1.66e-136 | 395 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036472.01.T01 | AT4G31500 | 46.037 | 328 | 171 | 4 | 1 | 324 | 174 | 499 | 3.35e-102 | 309 |
| MsG0780036472.01.T01 | AT3G26330 | 47.095 | 327 | 165 | 4 | 2 | 324 | 176 | 498 | 8.28e-100 | 303 |
| MsG0780036472.01.T01 | AT5G57260 | 47.239 | 326 | 162 | 6 | 1 | 319 | 174 | 496 | 8.92e-98 | 298 |
| MsG0780036472.01.T01 | AT3G26300 | 48.607 | 323 | 158 | 5 | 1 | 319 | 175 | 493 | 1.48e-96 | 295 |
| MsG0780036472.01.T01 | AT3G26320 | 46.177 | 327 | 168 | 4 | 2 | 324 | 176 | 498 | 1.63e-96 | 295 |
| MsG0780036472.01.T01 | AT3G26310 | 47.484 | 318 | 159 | 5 | 6 | 319 | 179 | 492 | 2.38e-94 | 289 |
| MsG0780036472.01.T01 | AT3G26290 | 46.037 | 328 | 169 | 4 | 1 | 324 | 188 | 511 | 3.29e-91 | 281 |
| MsG0780036472.01.T01 | AT3G26290 | 46.037 | 328 | 169 | 4 | 1 | 324 | 175 | 498 | 3.52e-91 | 281 |
| MsG0780036472.01.T01 | AT4G13770 | 41.796 | 323 | 182 | 4 | 1 | 319 | 175 | 495 | 4.07e-90 | 278 |
| MsG0780036472.01.T01 | AT1G13080 | 42.638 | 326 | 175 | 6 | 2 | 319 | 58 | 379 | 4.13e-90 | 274 |
Find 104 sgRNAs with CRISPR-Local
Find 165 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGAATAATACTAGTCA+AGG | 0.547152 | 7:+8635900 | None:intergenic |
| AAACCTTTACTGGTCTCTCT+TGG | 0.385897 | 7:+8634207 | None:intergenic |
| AAATATCACATGTGCGCTCT+CGG | 0.481709 | 7:+8635990 | None:intergenic |
| AAATTTCAGAGGAATGAACA+TGG | 0.670710 | 7:+8635445 | None:intergenic |
| AACATCTCAATCCTAATAAT+AGG | 0.353491 | 7:-8635165 | MsG0780036472.01.T01:CDS |
| AACCTTTACTGGTCTCTCTT+GGG | 0.300162 | 7:+8634208 | None:intergenic |
| AACTTCAGCCACATCGGTTT+GGG | 0.332182 | 7:-8634400 | MsG0780036472.01.T01:CDS |
| AAGGATGATATAGACATTGA+AGG | 0.510022 | 7:-8633907 | MsG0780036472.01.T01:CDS |
| AATATGATCTGAAACAAAGA+AGG | 0.548791 | 7:+8635285 | None:intergenic |
| AATATTGTCATACAATGGCA+TGG | 0.581793 | 7:-8635610 | MsG0780036472.01.T01:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAGTTATGAGCATGAAG+GGG | - | Chr7:8634277-8634296 | MsG0780036472.01.T01:CDS | 30.0% | |
| AAATTTCAGAGGAATGAACA+TGG | + | Chr7:8634197-8634216 | None:intergenic | 30.0% | |
| AACAAAGTGTAATCTCTCTT+TGG | - | Chr7:8634972-8634991 | MsG0780036472.01.T01:intron | 30.0% | |
| AAGGATGATATAGACATTGA+AGG | - | Chr7:8635732-8635751 | MsG0780036472.01.T01:five_prime_UTR | 30.0% | |
| AATATTGTCATACAATGGCA+TGG | - | Chr7:8634029-8634048 | MsG0780036472.01.T01:CDS | 30.0% | |
| AATCACATGTAAATGTCACA+AGG | - | Chr7:8635807-8635826 | MsG0780036472.01.T01:five_prime_UTR | 30.0% | |
| AATTTCCTAAACTCAATTGG+GGG | + | Chr7:8634780-8634799 | None:intergenic | 30.0% | |
| ACCTTCTTAACTCATTTGAT+TGG | - | Chr7:8635688-8635707 | MsG0780036472.01.T01:five_prime_UTR | 30.0% | |
| AGGAAAAAAAGAATGCTTGT+TGG | - | Chr7:8635827-8635846 | MsG0780036472.01.T01:five_prime_UTR | 30.0% | |
| AGGATGATATAGACATTGAA+GGG | - | Chr7:8635733-8635752 | MsG0780036472.01.T01:five_prime_UTR | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 8633651 | 8636010 | 8633651 | ID=MsG0780036472.01;Name=MsG0780036472.01 |
| Chr7 | mRNA | 8633651 | 8636010 | 8633651 | ID=MsG0780036472.01.T01;Parent=MsG0780036472.01;Name=MsG0780036472.01.T01;_AED=0.29;_eAED=0.29;_QI=585|1|1|1|1|1|2|192|324 |
| Chr7 | exon | 8633651 | 8634448 | 8633651 | ID=MsG0780036472.01.T01:exon:1392;Parent=MsG0780036472.01.T01 |
| Chr7 | exon | 8635057 | 8636010 | 8635057 | ID=MsG0780036472.01.T01:exon:1391;Parent=MsG0780036472.01.T01 |
| Chr7 | five_prime_UTR | 8635426 | 8636010 | 8635426 | ID=MsG0780036472.01.T01:five_prime_utr;Parent=MsG0780036472.01.T01 |
| Chr7 | CDS | 8635057 | 8635425 | 8635057 | ID=MsG0780036472.01.T01:cds;Parent=MsG0780036472.01.T01 |
| Chr7 | CDS | 8633843 | 8634448 | 8633843 | ID=MsG0780036472.01.T01:cds;Parent=MsG0780036472.01.T01 |
| Chr7 | three_prime_UTR | 8633651 | 8633842 | 8633651 | ID=MsG0780036472.01.T01:three_prime_utr;Parent=MsG0780036472.01.T01 |
| Gene Sequence |
>MsG0780036472.01.T01 ATGTCTGTCTTAAGCTCTATAGTGTGTAGAATTGCTTTTGGAAAAAGTTATGAGCATGAAGGGGATGAGAAAAGCAAGTTCCATAATTTACTTCATGAGACAGAAGCTATTTTCCTTTCCTTCTTTGTTTCAGATCATATTCCTTTTATGGGGTGGATTGATAAACTCACAGGGGCAAATGCTCGTGTTGACCAGACTTTCAAGGCTTTGGATGAGTTTCTTGAACAAGTCCTTAAGGAACATCTCAATCCTAATAATAGGAAGAAAGATGAAGAAGAGAAGGATATAGTTGACGTGTTACTCGAGTTGAAGAATCAGGGTCGTCTCTCAATTGATCTCACCAATGATCACATCAAAGCCGTCCTTATGAACCTACTTGTAGCAGCTACTGACACAACTTCAGCCACATCGGTTTGGGCGATGACTGGATTAATGAAGAATCCAAGAGCAATGAAGAAAGCTCGAGAAGAAATTAGAAACCTCTGCGGCAAAAAAGAGTTCATAGATGAAGATGATATTCAAAAGCTTGTATATCTTAAGGCTGTGATAAAAGAGACATTTAGATTCTATGCACCAGCGCCGCTAGCCCCAAGAGAGACCAGTAAAGGTTTCATTCTAAATGGATATAATATAGAACCAAAAACATCGGTGTTTGTGAATATTTGGGCCATTCATAGGGACCCTGAGGCATGGAAAGATCCCGACGAGTTTTATCCAGAGAGATTCTTAAACAATGATATAGATTTTAAGGGACGAGATTTTGAGTTGATACCATTTGGTGCAGGGCGTAGAATTTGCCCTGGCATGCCATTGGGAATCGCCACAGTGGAAATGATAATTGCAAACCTTCTTAACTCATTTGATTGGGAAATGCCTGAAGGGATGACAAAGGATGATATAGACATTGAAGGGTTGCCAGGACTTTCTAGACACAAGAAGAATCACCTTTGCCTTGTTGCCAAGAATCACATGTAA |
| Protein sequence |
>MsG0780036472.01.T01 MSVLSSIVCRIAFGKSYEHEGDEKSKFHNLLHETEAIFLSFFVSDHIPFMGWIDKLTGANARVDQTFKALDEFLEQVLKEHLNPNNRKKDEEEKDIVDVLLELKNQGRLSIDLTNDHIKAVLMNLLVAATDTTSATSVWAMTGLMKNPRAMKKAREEIRNLCGKKEFIDEDDIQKLVYLKAVIKETFRFYAPAPLAPRETSKGFILNGYNIEPKTSVFVNIWAIHRDPEAWKDPDEFYPERFLNNDIDFKGRDFELIPFGAGRRICPGMPLGIATVEMIIANLLNSFDWEMPEGMTKDDIDIEGLPGLSRHKKNHLCLVAKNHM* |
