AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031200.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031200.01.T01 MTR_6g017225 84.475 219 29 3 1 215 1 218 2.35e-133 379
MsG0680031200.01.T01 MTR_6g009650 49.289 211 92 6 1 205 1 202 1.26e-55 176
MsG0680031200.01.T01 MTR_6g066190 48.848 217 96 6 1 211 1 208 9.39e-54 172
MsG0680031200.01.T01 MTR_6g059730 51.786 224 91 9 1 216 1 215 1.47e-52 169
MsG0680031200.01.T01 MTR_6g009563 49.296 213 90 8 1 205 1 203 1.01e-51 166
MsG0680031200.01.T01 MTR_6g066210 45.872 218 103 5 1 211 1 210 5.45e-51 164
MsG0680031200.01.T01 MTR_6g059680 51.542 227 91 8 1 216 1 219 4.25e-49 160
MsG0680031200.01.T01 MTR_6g065460 43.478 207 101 6 7 205 6 204 6.10e-37 128
MsG0680031200.01.T01 MTR_6g059810 42.308 208 101 9 7 201 10 211 5.03e-33 118
MsG0680031200.01.T01 MTR_1g035770 39.524 210 100 11 1 192 1 201 9.25e-31 113
MsG0680031200.01.T01 MTR_3g463590 39.894 188 104 5 22 205 21 203 2.97e-28 106
MsG0680031200.01.T01 MTR_6g059530 40.000 220 108 11 1 213 1 203 6.10e-28 105
MsG0680031200.01.T01 MTR_6g065460 39.706 204 89 8 7 205 6 180 1.39e-27 103
MsG0680031200.01.T01 MTR_6g059650 41.209 182 92 9 28 202 26 199 2.24e-26 101
MsG0680031200.01.T01 MTR_6g059410 38.073 218 107 10 3 213 6 202 2.66e-26 100
MsG0680031200.01.T01 MTR_6g045433 35.321 218 117 10 1 203 1 209 1.08e-21 89.4
MsG0680031200.01.T01 MTR_6g059760 36.449 214 113 12 1 204 1 201 4.04e-21 87.8
MsG0680031200.01.T01 MTR_6g044810 35.814 215 117 12 1 204 1 205 3.46e-19 82.8
MsG0680031200.01.T01 MTR_6g044780 31.658 199 128 6 1 192 1 198 2.34e-18 80.5
MsG0680031200.01.T01 MTR_6g045097 32.323 198 126 6 1 191 1 197 2.79e-18 80.5
MsG0680031200.01.T01 MTR_1g057110 31.250 208 122 8 1 207 1 188 5.12e-17 76.3
MsG0680031200.01.T01 MTR_6g065570 51.515 66 31 1 11 75 7 72 3.12e-16 71.6
MsG0680031200.01.T01 MTR_6g045403 33.333 198 115 10 1 188 1 191 3.36e-16 74.7
MsG0680031200.01.T01 MTR_6g078290 29.208 202 125 5 1 186 1 200 2.51e-15 72.0
MsG0680031200.01.T01 MTR_2g073010 34.300 207 111 11 4 203 8 196 5.06e-14 68.6
MsG0680031200.01.T01 MTR_6g078070 31.792 173 106 8 10 173 4 173 6.81e-14 68.2
MsG0680031200.01.T01 MTR_6g078280 32.353 170 94 6 20 171 20 186 9.15e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGTCTTAGTCCACCTTTA+TGG 0.206660 6:+15923604 None:intergenic
TATCCTGTTGTCTTTGTTGA+TGG 0.232859 6:-15923171 MsG0680031200.01.T01:CDS
TAGTCTTAGTCCACCTTTAT+GGG 0.257214 6:+15923605 None:intergenic
AATGTGCCATGAAGTATTTC+AGG 0.267862 6:+15923351 None:intergenic
TGTGATGATTTAACACAATT+AGG 0.276879 6:+15923438 None:intergenic
CAAACGTGATCCACCATCTT+TGG 0.292305 6:+15923233 None:intergenic
CCAATAATAACATTAAAATT+AGG 0.332912 6:+15923393 None:intergenic
TATGAAAATGATTATAAGCT+TGG 0.341119 6:-15923315 MsG0680031200.01.T01:CDS
GACATTCCTGAAATACTTCA+TGG 0.349221 6:-15923357 MsG0680031200.01.T01:CDS
ACCTTTGCTTATTCCTTGTA+TGG 0.349643 6:+15923506 None:intergenic
TGGTGATGATAGAAAATATA+AGG 0.364732 6:+15923730 None:intergenic
CTCAAATGATGAACAAGTAT+TGG 0.381469 6:-15923691 MsG0680031200.01.T01:CDS
CCAGTGAAATTTACCATACA+AGG 0.401764 6:-15923519 MsG0680031200.01.T01:CDS
TCAACAAAGACAACAGGATA+AGG 0.426311 6:+15923174 None:intergenic
CCTTGTATGGTAAATTTCAC+TGG 0.434130 6:+15923519 None:intergenic
TCTTAGTGAACTCAATCTTA+AGG 0.438955 6:+15923463 None:intergenic
TATTCACCACCTGGGATAAG+AGG 0.444318 6:+15923654 None:intergenic
TGAGAAAGCTGATGAAAGAT+TGG 0.465855 6:+15923710 None:intergenic
AGAATTCCAGATTCAAAAGA+AGG 0.484094 6:+15923147 None:intergenic
GGACCATCAACAAAGACAAC+AGG 0.484741 6:+15923168 None:intergenic
AGAAAATATAAGGAAGGAAA+GGG 0.488938 6:+15923740 None:intergenic
GAACAAGTATTGGACACAAA+TGG 0.495359 6:-15923681 MsG0680031200.01.T01:CDS
CAACAAAGACAACAGGATAA+GGG 0.496475 6:+15923175 None:intergenic
AGCAAAGGTGTAATCTTGAC+AGG 0.504770 6:-15923492 MsG0680031200.01.T01:CDS
AGTCTTAGTCCACCTTTATG+GGG 0.513810 6:+15923606 None:intergenic
TAGAAAATATAAGGAAGGAA+AGG 0.516922 6:+15923739 None:intergenic
TTGCAAAATGAAAATATCAC+AGG 0.530508 6:-15923546 MsG0680031200.01.T01:CDS
AGATGGAATAATTCCAAAGA+TGG 0.539525 6:-15923246 MsG0680031200.01.T01:CDS
GATGATAGAAAATATAAGGA+AGG 0.547322 6:+15923734 None:intergenic
GTCTTAGTCCACCTTTATGG+GGG 0.571887 6:+15923607 None:intergenic
TGGAATAATTCCAAAGATGG+TGG 0.581969 6:-15923243 MsG0680031200.01.T01:CDS
ATTGTCTTGATATTATGAGA+TGG 0.582015 6:-15923263 MsG0680031200.01.T01:CDS
AAAATATAGTATTCACCACC+TGG 0.584351 6:+15923645 None:intergenic
AATGGTAACCCTCTTATCCC+AGG 0.597500 6:-15923663 MsG0680031200.01.T01:CDS
ATTCACCACCTGGGATAAGA+GGG 0.601650 6:+15923655 None:intergenic
ACCATACAAGGAATAAGCAA+AGG 0.616779 6:-15923507 MsG0680031200.01.T01:CDS
ATTGTGTTAAATCATCACAA+TGG 0.653286 6:-15923434 MsG0680031200.01.T01:CDS
AAATATAGTATTCACCACCT+GGG 0.666563 6:+15923646 None:intergenic
GGTAACCCTCTTATCCCAGG+TGG 0.707647 6:-15923660 MsG0680031200.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAATAATAACATTAAAATT+AGG + Chr6:15923500-15923519 None:intergenic 15.0%
!!! CCTAATTTTAATGTTATTAT+TGG - Chr6:15923497-15923516 MsG0680031200.01.T01:CDS 15.0%
!! TATGAAAATGATTATAAGCT+TGG - Chr6:15923575-15923594 MsG0680031200.01.T01:CDS 20.0%
!!! GATTTTGTAATGATGATTTT+GGG - Chr6:15923597-15923616 MsG0680031200.01.T01:CDS 20.0%
! AGAAAATATAAGGAAGGAAA+GGG + Chr6:15923153-15923172 None:intergenic 25.0%
! ATTGTCTTGATATTATGAGA+TGG - Chr6:15923627-15923646 MsG0680031200.01.T01:CDS 25.0%
! ATTGTGTTAAATCATCACAA+TGG - Chr6:15923456-15923475 MsG0680031200.01.T01:CDS 25.0%
! GATGATAGAAAATATAAGGA+AGG + Chr6:15923159-15923178 None:intergenic 25.0%
! TAGAAAATATAAGGAAGGAA+AGG + Chr6:15923154-15923173 None:intergenic 25.0%
! TGTGATGATTTAACACAATT+AGG + Chr6:15923455-15923474 None:intergenic 25.0%
! TTGCAAAATGAAAATATCAC+AGG - Chr6:15923344-15923363 MsG0680031200.01.T01:CDS 25.0%
!! GGATTTTGTAATGATGATTT+TGG - Chr6:15923596-15923615 MsG0680031200.01.T01:CDS 25.0%
!! TCATTTTGCAAGATAGTAAT+AGG + Chr6:15923335-15923354 None:intergenic 25.0%
!! TGGTGATGATAGAAAATATA+AGG + Chr6:15923163-15923182 None:intergenic 25.0%
!!! TTTTGAATCTGGAATTCTTA+TGG - Chr6:15923748-15923767 MsG0680031200.01.T01:CDS 25.0%
AAAATATAGTATTCACCACC+TGG + Chr6:15923248-15923267 None:intergenic 30.0%
AAATATAGTATTCACCACCT+GGG + Chr6:15923247-15923266 None:intergenic 30.0%
AGAATTCCAGATTCAAAAGA+AGG + Chr6:15923746-15923765 None:intergenic 30.0%
AGATGGAATAATTCCAAAGA+TGG - Chr6:15923644-15923663 MsG0680031200.01.T01:CDS 30.0%
CTCAAATGATGAACAAGTAT+TGG - Chr6:15923199-15923218 MsG0680031200.01.T01:CDS 30.0%
TCTTAGTGAACTCAATCTTA+AGG + Chr6:15923430-15923449 None:intergenic 30.0%
ACCATACAAGGAATAAGCAA+AGG - Chr6:15923383-15923402 MsG0680031200.01.T01:CDS 35.0%
ACCTTTGCTTATTCCTTGTA+TGG + Chr6:15923387-15923406 None:intergenic 35.0%
CAACAAAGACAACAGGATAA+GGG + Chr6:15923718-15923737 None:intergenic 35.0%
CCAGTGAAATTTACCATACA+AGG - Chr6:15923371-15923390 MsG0680031200.01.T01:CDS 35.0%
CCTTGTATGGTAAATTTCAC+TGG + Chr6:15923374-15923393 None:intergenic 35.0%
GACATTCCTGAAATACTTCA+TGG - Chr6:15923533-15923552 MsG0680031200.01.T01:CDS 35.0%
TATCCTGTTGTCTTTGTTGA+TGG - Chr6:15923719-15923738 MsG0680031200.01.T01:CDS 35.0%
TCAACAAAGACAACAGGATA+AGG + Chr6:15923719-15923738 None:intergenic 35.0%
TGAGAAAGCTGATGAAAGAT+TGG + Chr6:15923183-15923202 None:intergenic 35.0%
TGGAATAATTCCAAAGATGG+TGG - Chr6:15923647-15923666 MsG0680031200.01.T01:CDS 35.0%
! AATGTGCCATGAAGTATTTC+AGG + Chr6:15923542-15923561 None:intergenic 35.0%
! TAGTCTTAGTCCACCTTTAT+GGG + Chr6:15923288-15923307 None:intergenic 35.0%
!! GAACAAGTATTGGACACAAA+TGG - Chr6:15923209-15923228 MsG0680031200.01.T01:CDS 35.0%
AGTCTTAGTCCACCTTTATG+GGG + Chr6:15923287-15923306 None:intergenic 40.0%
GGACTAAGACTAGCAAAAAC+TGG - Chr6:15923296-15923315 MsG0680031200.01.T01:CDS 40.0%
! AGCAAAGGTGTAATCTTGAC+AGG - Chr6:15923398-15923417 MsG0680031200.01.T01:CDS 40.0%
! GATGGTCCTTCTTTTGAATC+TGG - Chr6:15923737-15923756 MsG0680031200.01.T01:CDS 40.0%
!! CTAGTCTTAGTCCACCTTTA+TGG + Chr6:15923289-15923308 None:intergenic 40.0%
AATGGTAACCCTCTTATCCC+AGG - Chr6:15923227-15923246 MsG0680031200.01.T01:CDS 45.0%
ATTCACCACCTGGGATAAGA+GGG + Chr6:15923238-15923257 None:intergenic 45.0%
CAAACGTGATCCACCATCTT+TGG + Chr6:15923660-15923679 None:intergenic 45.0%
GGACCATCAACAAAGACAAC+AGG + Chr6:15923725-15923744 None:intergenic 45.0%
GTCTTAGTCCACCTTTATGG+GGG + Chr6:15923286-15923305 None:intergenic 45.0%
TATTCACCACCTGGGATAAG+AGG + Chr6:15923239-15923258 None:intergenic 45.0%
ACTCAAGACCCCCATAAAGG+TGG - Chr6:15923275-15923294 MsG0680031200.01.T01:CDS 50.0%
GCAACTCAAGACCCCCATAA+AGG - Chr6:15923272-15923291 MsG0680031200.01.T01:CDS 50.0%
! TTATGGGGGTCTTGAGTTGC+TGG + Chr6:15923272-15923291 None:intergenic 50.0%
GGTAACCCTCTTATCCCAGG+TGG - Chr6:15923230-15923249 MsG0680031200.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 15923128 15923784 15923128 ID=MsG0680031200.01;Name=MsG0680031200.01
Chr6 mRNA 15923128 15923784 15923128 ID=MsG0680031200.01.T01;Parent=MsG0680031200.01;Name=MsG0680031200.01.T01;_AED=0.20;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|218
Chr6 exon 15923128 15923784 15923128 ID=MsG0680031200.01.T01:exon:10668;Parent=MsG0680031200.01.T01
Chr6 CDS 15923128 15923784 15923128 ID=MsG0680031200.01.T01:cds;Parent=MsG0680031200.01.T01
Gene Sequence

>MsG0680031200.01.T01

ATGAAGCTAGTTTTATCACTCACCCTTTCCTTCCTTATATTTTCTATCATCACCAATCTTTCATCAGCTTTCTCAAATGATGAACAAGTATTGGACACAAATGGTAACCCTCTTATCCCAGGTGGTGAATACTATATTTTTCCAGCAACTCAAGACCCCCATAAAGGTGGACTAAGACTAGCAAAAACTGGTGATTCAAAGTGTCCTATTACTATCTTGCAAAATGAAAATATCACAGGTTTACCAGTGAAATTTACCATACAAGGAATAAGCAAAGGTGTAATCTTGACAGGTACTGACCTTAAGATTGAGTTCACTAAGAAGCCTAATTGTGTTAAATCATCACAATGGTTAATGTTTTATGATTTTCCTAATTTTAATGTTATTATTGGTGCTTTTGATTCAGACATTCCTGAAATACTTCATGGCACATTTTATATTCAGAAATATGAAAATGATTATAAGCTTGGATTTTGTAATGATGATTTTGGGAAACAAAATTGTCTTGATATTATGAGATGGAATAATTCCAAAGATGGTGGATCACGTTTGAGATTGATTTTGAAAGCTGATCAAGAAGTACAAGACCCTTATCCTGTTGTCTTTGTTGATGGTCCTTCTTTTGAATCTGGAATTCTTATGGATGTGAGACTTTGA

Protein sequence

>MsG0680031200.01.T01

MKLVLSLTLSFLIFSIITNLSSAFSNDEQVLDTNGNPLIPGGEYYIFPATQDPHKGGLRLAKTGDSKCPITILQNENITGLPVKFTIQGISKGVILTGTDLKIEFTKKPNCVKSSQWLMFYDFPNFNVIIGAFDSDIPEILHGTFYIQKYENDYKLGFCNDDFGKQNCLDIMRWNNSKDGGSRLRLILKADQEVQDPYPVVFVDGPSFESGILMDVRL*