AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022489.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022489.01.T01 MTR_4g090620 98.095 105 2 0 10 114 382 486 3.15e-69 219
MsG0480022489.01.T01 MTR_4g090610 84.615 52 8 0 137 188 75 126 1.73e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022489.01.T01 AT3G06350 71.296 108 31 0 10 117 487 594 1.51e-49 169

Find 38 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCACAAAGTTTCTAAAA+TGG 0.226725 4:-73179977 MsG0480022489.01.T01:CDS
ACAGGACTGGAGATGTTTAT+TGG 0.265366 4:-73182708 MsG0480022489.01.T01:CDS
TAGGAAATCGTGAAAATAAT+AGG 0.297114 4:-73182048 MsG0480022489.01.T01:CDS
GTTGCCCACTGTTCTGGCTT+GGG 0.302142 4:-73179899 MsG0480022489.01.T01:CDS
ACAGGTGTTTCATCAACTTT+TGG 0.331032 4:+73183558 None:intergenic
GTAGTGTAGTGAAGAAGGTT+AGG 0.342020 4:+73179833 None:intergenic
CGACAATATGAGCATTATAC+TGG 0.370012 4:-73182675 MsG0480022489.01.T01:CDS
ACTGGCGTTGCCCACTGTTC+TGG 0.379196 4:-73179905 MsG0480022489.01.T01:CDS
TTGAAAGAAGCAGAAGAATC+TGG 0.381681 4:-73182744 MsG0480022489.01.T01:CDS
CGTTGCCCACTGTTCTGGCT+TGG 0.384296 4:-73179900 MsG0480022489.01.T01:CDS
TCTGGAGCCACTATTGTAAC+AGG 0.414913 4:-73182726 MsG0480022489.01.T01:CDS
TTATACAAACTATTGGAAAC+AGG 0.420896 4:-73182005 MsG0480022489.01.T01:CDS
GAGGAGTAGTGTAGTGAAGA+AGG 0.425024 4:+73179828 None:intergenic
AAATAGCATACCTTTGAAAC+AGG 0.438226 4:+73183540 None:intergenic
TGCAATTTGTAAAATTTCAC+AGG 0.444507 4:-73179860 MsG0480022489.01.T01:CDS
ACAAAGTTTCTAAAATGGTT+CGG 0.451645 4:-73179972 MsG0480022489.01.T01:CDS
GATTACATCAGCAAGTTCTC+TGG 0.463591 4:+73183671 None:intergenic
TAGTGTAGTGAAGAAGGTTA+GGG 0.470147 4:+73179834 None:intergenic
CTAGCAAACACAACTTCAAT+CGG 0.472513 4:-73183588 MsG0480022489.01.T01:CDS
AGTTCCACTGCTGCTATCAC+TGG 0.478428 4:-73179923 MsG0480022489.01.T01:CDS
GAGCATTATACTGGATTGCC+AGG 0.481705 4:-73182666 MsG0480022489.01.T01:intron
TTGCCCACTGTTCTGGCTTG+GGG 0.483534 4:-73179898 MsG0480022489.01.T01:CDS
CTCCAGTCCTGTTACAATAG+TGG 0.488995 4:+73182719 None:intergenic
GCTAGAATCATACCATCCTC+TGG 0.504787 4:+73183606 None:intergenic
AGAGAACTTGCTGATGTAAT+CGG 0.508494 4:-73183669 MsG0480022489.01.T01:CDS
GTTCTGGCTTGGGGAAAAGA+TGG 0.514348 4:-73179889 MsG0480022489.01.T01:CDS
TGATGAAACACCTGTTTCAA+AGG 0.525212 4:-73183550 MsG0480022489.01.T01:intron
GATAATTACCATCCAGAGGA+TGG 0.527848 4:-73183618 MsG0480022489.01.T01:CDS
AGCCACTATTGTAACAGGAC+TGG 0.563539 4:-73182721 MsG0480022489.01.T01:CDS
TTTCCCCAAGCCAGAACAGT+GGG 0.572584 4:+73179895 None:intergenic
GAATCATACCATCCTCTGGA+TGG 0.586059 4:+73183610 None:intergenic
ATCAAAGGATGAATTAGGTG+AGG 0.600745 4:+73179809 None:intergenic
GTAATTATCTAAATCACTGA+GGG 0.622651 4:+73183632 None:intergenic
TTTAGATAATTACCATCCAG+AGG 0.639923 4:-73183622 MsG0480022489.01.T01:CDS
GAACTTGCTGATGTAATCGG+TGG 0.640214 4:-73183666 MsG0480022489.01.T01:CDS
AACGCCAGTGATAGCAGCAG+TGG 0.653347 4:+73179919 None:intergenic
TACAAACTATTGGAAACAGG+AGG 0.660877 4:-73182002 MsG0480022489.01.T01:intron
GGTAATTATCTAAATCACTG+AGG 0.690501 4:+73183631 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTGAATAGTAAAAT+TGG + Chr4:73183227-73183246 None:intergenic 10.0%
!! TATATAATAGTAAATTGAAA+AGG + Chr4:73181644-73181663 None:intergenic 10.0%
!!! TATTAATTTTTTTTTGTTTG+GGG + Chr4:73183100-73183119 None:intergenic 10.0%
!! TTAACCATTAAAATTAATTG+CGG + Chr4:73182292-73182311 None:intergenic 15.0%
!!! AATTTTTAGTTTTTACAGAA+TGG + Chr4:73182211-73182230 None:intergenic 15.0%
!!! AGCAAGTATATATTTTTTTA+AGG - Chr4:73181374-73181393 MsG0480022489.01.T01:intron 15.0%
!!! ATTAATTTTTTTTTGTTTGG+GGG + Chr4:73183099-73183118 None:intergenic 15.0%
!!! CAATTTTTTATACAAACTAT+TGG - Chr4:73181515-73181534 MsG0480022489.01.T01:intron 15.0%
!!! GTCAAATATTGTTTTTATTA+GGG - Chr4:73180625-73180644 MsG0480022489.01.T01:intron 15.0%
!!! TACTATTCAATTTTTTTTGA+GGG - Chr4:73183231-73183250 MsG0480022489.01.T01:intron 15.0%
!!! TATGTAAGAATATTTTATAG+TGG - Chr4:73180306-73180325 MsG0480022489.01.T01:intron 15.0%
!!! TGTCAAATATTGTTTTTATT+AGG - Chr4:73180624-73180643 MsG0480022489.01.T01:intron 15.0%
!!! TTACTATTCAATTTTTTTTG+AGG - Chr4:73183230-73183249 MsG0480022489.01.T01:intron 15.0%
!! ACAATACAATACAAATTACA+AGG + Chr4:73182059-73182078 None:intergenic 20.0%
!! ACTATGATTGTGTATTTATT+TGG - Chr4:73180450-73180469 MsG0480022489.01.T01:intron 20.0%
!! AGTAATATTATTTAGTACGA+AGG + Chr4:73182832-73182851 None:intergenic 20.0%
!! ATTAAAGAAAAATACTAGCA+AGG - Chr4:73182139-73182158 MsG0480022489.01.T01:intron 20.0%
!! CATAAATATTTCAAACCAAA+AGG + Chr4:73180427-73180446 None:intergenic 20.0%
!! TAACCATTAAAATTAATTGC+GGG + Chr4:73182291-73182310 None:intergenic 20.0%
!! TGCTTACAGATTAAATTATA+TGG - Chr4:73180332-73180351 MsG0480022489.01.T01:intron 20.0%
!!! AATTTGAAGAATGGAATTTT+AGG - Chr4:73183520-73183539 MsG0480022489.01.T01:intron 20.0%
!!! ATTAGTTTTAGAAGTTTTCA+CGG - Chr4:73182228-73182247 MsG0480022489.01.T01:intron 20.0%
!!! TTAAAGAATGAGTATTTTGA+TGG + Chr4:73180516-73180535 None:intergenic 20.0%
! AAATTTAATAGACTATGCCA+TGG - Chr4:73180899-73180918 MsG0480022489.01.T01:intron 25.0%
! AACAATTGAGCATAAAACAA+AGG + Chr4:73181326-73181345 None:intergenic 25.0%
! AAGTATAAAGTTCTGTTTAG+AGG + Chr4:73181613-73181632 None:intergenic 25.0%
! AATATCACAATATGCTATGT+TGG - Chr4:73180190-73180209 MsG0480022489.01.T01:intron 25.0%
! AGAACTCTTCAGAAATATTA+TGG - Chr4:73181037-73181056 MsG0480022489.01.T01:intron 25.0%
! AGTATAAAGTTCTGTTTAGA+GGG + Chr4:73181612-73181631 None:intergenic 25.0%
! ATGAGAAATGTTGAACATTA+TGG + Chr4:73183369-73183388 None:intergenic 25.0%
! ATGTTCAACATTTCTCATAT+AGG - Chr4:73183371-73183390 MsG0480022489.01.T01:intron 25.0%
! CAAAATACTCATTCTTTAAG+TGG - Chr4:73180517-73180536 MsG0480022489.01.T01:intron 25.0%
! GATAATATATGCAATGTACA+TGG + Chr4:73182731-73182750 None:intergenic 25.0%
! GCAATACAATTTCAATTTCA+AGG - Chr4:73183140-73183159 MsG0480022489.01.T01:intron 25.0%
! GTAATTATCTAAATCACTGA+GGG + Chr4:73179898-73179917 None:intergenic 25.0%
! TAGGAAATCGTGAAAATAAT+AGG - Chr4:73181479-73181498 MsG0480022489.01.T01:intron 25.0%
! TCAAATAATCACTCAAGAAA+GGG - Chr4:73182640-73182659 MsG0480022489.01.T01:intron 25.0%
! TCATATGTTCCTTAGTTAAT+TGG - Chr4:73182672-73182691 MsG0480022489.01.T01:CDS 25.0%
! TGCAATTTGTAAAATTTCAC+AGG - Chr4:73183667-73183686 MsG0480022489.01.T01:CDS 25.0%
! TTATACAAACTATTGGAAAC+AGG - Chr4:73181522-73181541 MsG0480022489.01.T01:intron 25.0%
! TTCAAATAATCACTCAAGAA+AGG - Chr4:73182639-73182658 MsG0480022489.01.T01:intron 25.0%
! TTCTACCACATATTTATGTT+TGG - Chr4:73180569-73180588 MsG0480022489.01.T01:intron 25.0%
!! AGTAAAATTGGTTCTCAAAA+TGG + Chr4:73183215-73183234 None:intergenic 25.0%
!! TATCCCGCAATTAATTTTAA+TGG - Chr4:73182285-73182304 MsG0480022489.01.T01:intron 25.0%
!! TCAAAGGTATGCTATTTATA+TGG - Chr4:73179993-73180012 MsG0480022489.01.T01:intron 25.0%
!!! AACCATTTTAGAAACTTTGT+GGG + Chr4:73183555-73183574 None:intergenic 25.0%
!!! AATTGGACGGTTTTTTTTTT+TGG - Chr4:73182689-73182708 MsG0480022489.01.T01:CDS 25.0%
!!! ACAAAGTTTCTAAAATGGTT+CGG - Chr4:73183555-73183574 MsG0480022489.01.T01:CDS 25.0%
!!! ATATTTCTGAAGAGTTCTTT+TGG + Chr4:73181035-73181054 None:intergenic 25.0%
!!! ATGTTATTTTCCTTGAATAG+TGG - Chr4:73182357-73182376 MsG0480022489.01.T01:intron 25.0%
!!! ATTGGACGGTTTTTTTTTTT+GGG - Chr4:73182690-73182709 MsG0480022489.01.T01:CDS 25.0%
!!! TTCAATTTTTTCAACGTAGA+TGG - Chr4:73181982-73182001 MsG0480022489.01.T01:intron 25.0%
!!! TTGATTTTTCTAGTGAAGAT+TGG - Chr4:73180092-73180111 MsG0480022489.01.T01:intron 25.0%
AAAAACCGTCCAATTAACTA+AGG + Chr4:73182684-73182703 None:intergenic 30.0%
AACTAAGGAACATATGACTT+TGG + Chr4:73182669-73182688 None:intergenic 30.0%
ACTTCAAGTATGTATGTTCA+GGG + Chr4:73183491-73183510 None:intergenic 30.0%
ATATCACGAGATATGTATCA+CGG - Chr4:73182874-73182893 MsG0480022489.01.T01:intron 30.0%
ATGTTCCTTAGTTAATTGGA+CGG - Chr4:73182676-73182695 MsG0480022489.01.T01:CDS 30.0%
ATTATGGAAAACTATTGACG+AGG - Chr4:73181053-73181072 MsG0480022489.01.T01:intron 30.0%
CAAAATTCTGCTAAGGAAAT+GGG + Chr4:73180363-73180382 None:intergenic 30.0%
CACGATTTCCTATACAAAAT+AGG + Chr4:73181471-73181490 None:intergenic 30.0%
CTTACAAATTCCACTATTCA+AGG + Chr4:73182370-73182389 None:intergenic 30.0%
CTTCGCATTCTTAATGTAAT+AGG - Chr4:73181810-73181829 MsG0480022489.01.T01:intron 30.0%
GAACATTATGGATATCTCTT+TGG + Chr4:73183357-73183376 None:intergenic 30.0%
GATACTTGTGAAGATATTCT+AGG + Chr4:73180259-73180278 None:intergenic 30.0%
GGTAATTATCTAAATCACTG+AGG + Chr4:73179899-73179918 None:intergenic 30.0%
GTAGTGAATGTAACTATCTT+TGG - Chr4:73181181-73181200 MsG0480022489.01.T01:intron 30.0%
GTTCGAAGTTAGAATAAAAG+AGG - Chr4:73182449-73182468 MsG0480022489.01.T01:intron 30.0%
TGAACAAAATGCCATAAACT+TGG - Chr4:73180490-73180509 MsG0480022489.01.T01:intron 30.0%
TTTAGATAATTACCATCCAG+AGG - Chr4:73179905-73179924 MsG0480022489.01.T01:CDS 30.0%
! AACATTACCTTTTCAACGTT+AGG + Chr4:73181860-73181879 None:intergenic 30.0%
! ATTTGAAGTAGAACATGCAT+GGG + Chr4:73182626-73182645 None:intergenic 30.0%
! CTGCATTTCCTATTTTGTAT+AGG - Chr4:73181460-73181479 MsG0480022489.01.T01:intron 30.0%
! GAGAGTGTAAATTTGAAGAA+TGG - Chr4:73183511-73183530 MsG0480022489.01.T01:intron 30.0%
! TATTTGAAGTAGAACATGCA+TGG + Chr4:73182627-73182646 None:intergenic 30.0%
! TGCATTGAAATTTTACTCGT+TGG - Chr4:73180722-73180741 MsG0480022489.01.T01:intron 30.0%
! TTCCCACAAAGTTTCTAAAA+TGG - Chr4:73183550-73183569 MsG0480022489.01.T01:intron 30.0%
!! AAATAGCATACCTTTGAAAC+AGG + Chr4:73179990-73180009 None:intergenic 30.0%
!! GAACCATTTTAGAAACTTTG+TGG + Chr4:73183556-73183575 None:intergenic 30.0%
!! TTCAAGAGTCTAGTCATTTT+TGG + Chr4:73180768-73180787 None:intergenic 30.0%
!! TTCTCTAAAGCAATCATGTA+TGG + Chr4:73181160-73181179 None:intergenic 30.0%
!! TTGTAATGACTTGTACCTTT+TGG - Chr4:73180409-73180428 MsG0480022489.01.T01:intron 30.0%
!!! CATTCGATGTTTTAACTTCA+AGG - Chr4:73181937-73181956 MsG0480022489.01.T01:intron 30.0%
!!! TATTTTGATGGCCAAGTTTA+TGG + Chr4:73180504-73180523 None:intergenic 30.0%
!!! TTTTAAAGCTGAAGAAGCAT+TGG - Chr4:73182091-73182110 MsG0480022489.01.T01:intron 30.0%
AAAGTACAAACCACTGAAAC+AGG + Chr4:73180950-73180969 None:intergenic 35.0%
AACTCACGAGGACAATTAAT+TGG - Chr4:73182774-73182793 MsG0480022489.01.T01:CDS 35.0%
AAGTACAAACCACTGAAACA+GGG + Chr4:73180949-73180968 None:intergenic 35.0%
AATAGGACGACAACAATAAG+AGG - Chr4:73181827-73181846 MsG0480022489.01.T01:intron 35.0%
AATGTACATGGTTCAAACTC+CGG + Chr4:73182719-73182738 None:intergenic 35.0%
ACAGGCCAAACATAAATATG+TGG + Chr4:73180577-73180596 None:intergenic 35.0%
ACATATTTATGTTTGGCCTG+TGG - Chr4:73180576-73180595 MsG0480022489.01.T01:intron 35.0%
ACTCATTCTTTAAGTGGAAG+TGG - Chr4:73180523-73180542 MsG0480022489.01.T01:intron 35.0%
AGAAGTAATTAGTTCCCTGA+AGG + Chr4:73180687-73180706 None:intergenic 35.0%
AGTAAATTGAAAAGGTGAGC+AGG + Chr4:73181636-73181655 None:intergenic 35.0%
ATGGCTGTACTAAACCAATT+AGG - Chr4:73180012-73180031 MsG0480022489.01.T01:intron 35.0%
CACTTCAAGTATGTATGTTC+AGG + Chr4:73183492-73183511 None:intergenic 35.0%
CCAAAATTCTGCTAAGGAAA+TGG + Chr4:73180364-73180383 None:intergenic 35.0%
CCACTAACAATAATAGCACA+AGG + Chr4:73180039-73180058 None:intergenic 35.0%
CCTAATACCTAACGTTGAAA+AGG - Chr4:73181850-73181869 MsG0480022489.01.T01:intron 35.0%
CGACAATATGAGCATTATAC+TGG - Chr4:73180852-73180871 MsG0480022489.01.T01:intron 35.0%
CTAGCAAACACAACTTCAAT+CGG - Chr4:73179939-73179958 MsG0480022489.01.T01:CDS 35.0%
GATCAGTGAGATAGAAGTTA+GGG + Chr4:73181087-73181106 None:intergenic 35.0%
GGTGATAACTTTCTATACGA+AGG + Chr4:73181117-73181136 None:intergenic 35.0%
GTGATGTAGACCAACAAAAA+TGG + Chr4:73182542-73182561 None:intergenic 35.0%
TACAAACTATTGGAAACAGG+AGG - Chr4:73181525-73181544 MsG0480022489.01.T01:intron 35.0%
TAGGTTGCATGATTCACAAA+GGG + Chr4:73182584-73182603 None:intergenic 35.0%
TAGTGTAGTGAAGAAGGTTA+GGG + Chr4:73183696-73183715 None:intergenic 35.0%
TATCACGAGATATGTATCAC+GGG - Chr4:73182875-73182894 MsG0480022489.01.T01:intron 35.0%
TCTTAATCCTAGAAGTAGGA+TGG - Chr4:73181898-73181917 MsG0480022489.01.T01:intron 35.0%
TGATCAGTGAGATAGAAGTT+AGG + Chr4:73181088-73181107 None:intergenic 35.0%
TGATGAAACACCTGTTTCAA+AGG - Chr4:73179977-73179996 MsG0480022489.01.T01:CDS 35.0%
TGCATCTTAATCCTAGAAGT+AGG - Chr4:73181894-73181913 MsG0480022489.01.T01:intron 35.0%
TGGCTGTACTAAACCAATTA+GGG - Chr4:73180013-73180032 MsG0480022489.01.T01:intron 35.0%
TTGAAAGAAGCAGAAGAATC+TGG - Chr4:73180783-73180802 MsG0480022489.01.T01:intron 35.0%
TTGTGCCAAATGACAATTAG+CGG + Chr4:73182417-73182436 None:intergenic 35.0%
TTTCAAAGGATAATGTGTCG+AGG + Chr4:73181745-73181764 None:intergenic 35.0%
! ACAGGTGTTTCATCAACTTT+TGG + Chr4:73179972-73179991 None:intergenic 35.0%
! CCATTTCCTTAGCAGAATTT+TGG - Chr4:73180361-73180380 MsG0480022489.01.T01:intron 35.0%
! CCTTGTGCTATTATTGTTAG+TGG - Chr4:73180036-73180055 MsG0480022489.01.T01:intron 35.0%
! CCTTTTCAACGTTAGGTATT+AGG + Chr4:73181853-73181872 None:intergenic 35.0%
! GCGTCATGATGTGATTTTAT+TGG + Chr4:73180218-73180237 None:intergenic 35.0%
! TTAGGTTGCATGATTCACAA+AGG + Chr4:73182585-73182604 None:intergenic 35.0%
! TTCTAGGAACCAACTTAACA+CGG + Chr4:73180243-73180262 None:intergenic 35.0%
!! AGAGAACTTGCTGATGTAAT+CGG - Chr4:73179858-73179877 MsG0480022489.01.T01:CDS 35.0%
!! CACATTATCCTTTGAAACAC+AGG - Chr4:73181748-73181767 MsG0480022489.01.T01:intron 35.0%
!! CTATGTGCAACCATTTTTGT+TGG - Chr4:73182529-73182548 MsG0480022489.01.T01:intron 35.0%
!! TTCCTTAGCAGAATTTTGGT+TGG - Chr4:73180365-73180384 MsG0480022489.01.T01:intron 35.0%
!!! TCCTTAGCAGAATTTTGGTT+GGG - Chr4:73180366-73180385 MsG0480022489.01.T01:intron 35.0%
!!! TGGTAGAAGTGCTTTTGTTT+GGG + Chr4:73180557-73180576 None:intergenic 35.0%
!!! TTTTTTACTGTCAGCTTACC+TGG + Chr4:73180882-73180901 None:intergenic 35.0%
AAACAGGGACAACGAAATCA+GGG + Chr4:73180934-73180953 None:intergenic 40.0%
AACTTCAAGGTTGTAAACGC+TGG - Chr4:73181950-73181969 MsG0480022489.01.T01:intron 40.0%
ACAGGACTGGAGATGTTTAT+TGG - Chr4:73180819-73180838 MsG0480022489.01.T01:intron 40.0%
AGCAATCATGTATGGAGTAC+TGG + Chr4:73181152-73181171 None:intergenic 40.0%
AGCTCACTTGGTATGAACAA+TGG + Chr4:73182753-73182772 None:intergenic 40.0%
CACGAGGACAATTAATTGGA+CGG - Chr4:73182778-73182797 MsG0480022489.01.T01:CDS 40.0%
CTATGATGAGATGCAATTGC+TGG - Chr4:73180278-73180297 MsG0480022489.01.T01:intron 40.0%
GATAATTACCATCCAGAGGA+TGG - Chr4:73179909-73179928 MsG0480022489.01.T01:CDS 40.0%
GATTACATCAGCAAGTTCTC+TGG + Chr4:73179859-73179878 None:intergenic 40.0%
GCAATCCGCTAATTGTCATT+TGG - Chr4:73182409-73182428 MsG0480022489.01.T01:intron 40.0%
GTAGTGTAGTGAAGAAGGTT+AGG + Chr4:73183697-73183716 None:intergenic 40.0%
GTCGAGGATAATAAACAGAC+AGG + Chr4:73181729-73181748 None:intergenic 40.0%
GTCTGTAACCTGTGTTTCAA+AGG + Chr4:73181759-73181778 None:intergenic 40.0%
TAATAGCACAAGGCCCTAAT+TGG + Chr4:73180029-73180048 None:intergenic 40.0%
TCCCAACCAAAATTCTGCTA+AGG + Chr4:73180370-73180389 None:intergenic 40.0%
TGTGGCGTTTCTATTCTAGA+TGG - Chr4:73180594-73180613 MsG0480022489.01.T01:intron 40.0%
TTCTTTAAGTGGAAGTGGAG+CGG - Chr4:73180528-73180547 MsG0480022489.01.T01:intron 40.0%
TTGAAACACAGGTTACAGAC+AGG - Chr4:73181759-73181778 MsG0480022489.01.T01:intron 40.0%
TTGCATGTGTGACAACTCAA+CGG - Chr4:73182182-73182201 MsG0480022489.01.T01:intron 40.0%
! AGACTATGCCATGGACTTTT+TGG - Chr4:73180908-73180927 MsG0480022489.01.T01:intron 40.0%
! ATCTAGAATAGAAACGCCAC+AGG + Chr4:73180595-73180614 None:intergenic 40.0%
! ATGAGAGTAAGTTGACCTTC+AGG - Chr4:73180669-73180688 MsG0480022489.01.T01:intron 40.0%
! TGAGAGTAAGTTGACCTTCA+GGG - Chr4:73180670-73180689 MsG0480022489.01.T01:intron 40.0%
!! GAGTACTGGATATAAGCACA+AGG + Chr4:73181138-73181157 None:intergenic 40.0%
!!! GGACGGTTTTTTTTTTTGGG+TGG - Chr4:73182693-73182712 MsG0480022489.01.T01:CDS 40.0%
!!! GGTTTTTTTTTTTGGGTGGC+CGG - Chr4:73182697-73182716 MsG0480022489.01.T01:CDS 40.0%
!!! GTGGTAGAAGTGCTTTTGTT+TGG + Chr4:73180558-73180577 None:intergenic 40.0%
AATCAGGGCCAAAAAGTCCA+TGG + Chr4:73180919-73180938 None:intergenic 45.0%
AGCCACTATTGTAACAGGAC+TGG - Chr4:73180806-73180825 MsG0480022489.01.T01:intron 45.0%
CTCCAGTCCTGTTACAATAG+TGG + Chr4:73180811-73180830 None:intergenic 45.0%
GAAACAGGGACAACGAAATC+AGG + Chr4:73180935-73180954 None:intergenic 45.0%
GAATCATACCATCCTCTGGA+TGG + Chr4:73179920-73179939 None:intergenic 45.0%
GAGCATTATACTGGATTGCC+AGG - Chr4:73180861-73180880 MsG0480022489.01.T01:intron 45.0%
GAGGAGTAGTGTAGTGAAGA+AGG + Chr4:73183702-73183721 None:intergenic 45.0%
GCTAGAATCATACCATCCTC+TGG + Chr4:73179924-73179943 None:intergenic 45.0%
TAGGAACCAACTTAACACGG+TGG + Chr4:73180240-73180259 None:intergenic 45.0%
TGCAAACGCTCATCAAAAGC+AGG + Chr4:73182167-73182186 None:intergenic 45.0%
TTCACAAAGGGTCTCACATG+AGG + Chr4:73182572-73182591 None:intergenic 45.0%
TTTCGTTGTCCCTGTTTCAG+TGG - Chr4:73180937-73180956 MsG0480022489.01.T01:intron 45.0%
! CAGTGAGGTGGAGAGTTTTT+AGG + Chr4:73182603-73182622 None:intergenic 45.0%
! TCTGGAGCCACTATTGTAAC+AGG - Chr4:73180801-73180820 MsG0480022489.01.T01:intron 45.0%
!! GAACTTGCTGATGTAATCGG+TGG - Chr4:73179861-73179880 MsG0480022489.01.T01:CDS 45.0%
!!! TTATTAATTTTTTTTTGTTT+GGG + Chr4:73183101-73183120 None:intergenic 5.0%
!!! TTTATTAATTTTTTTTTGTT+TGG + Chr4:73183102-73183121 None:intergenic 5.0%
AGTTCCACTGCTGCTATCAC+TGG - Chr4:73183604-73183623 MsG0480022489.01.T01:CDS 50.0%
ATGACGCCACCGTGTTAAGT+TGG - Chr4:73180231-73180250 MsG0480022489.01.T01:intron 50.0%
CCAAGTGAGCTAAACTCACG+AGG - Chr4:73182762-73182781 MsG0480022489.01.T01:CDS 50.0%
CTGCTCACCATCCTACTTCT+AGG + Chr4:73181908-73181927 None:intergenic 50.0%
TAGAACATGCATGGGCAGTG+AGG + Chr4:73182618-73182637 None:intergenic 50.0%
TTTCCCCAAGCCAGAACAGT+GGG + Chr4:73183635-73183654 None:intergenic 50.0%
! CCTCGTGAGTTTAGCTCACT+TGG + Chr4:73182765-73182784 None:intergenic 50.0%
! TTTTCCCCAAGCCAGAACAG+TGG + Chr4:73183636-73183655 None:intergenic 50.0%
!! GTTCTGGCTTGGGGAAAAGA+TGG - Chr4:73183638-73183657 MsG0480022489.01.T01:CDS 50.0%
AACATGCATGGGCAGTGAGG+TGG + Chr4:73182615-73182634 None:intergenic 55.0%
AACGCCAGTGATAGCAGCAG+TGG + Chr4:73183611-73183630 None:intergenic 55.0%
!! GTTGCCCACTGTTCTGGCTT+GGG - Chr4:73183628-73183647 MsG0480022489.01.T01:CDS 55.0%
!! TTGCCCACTGTTCTGGCTTG+GGG - Chr4:73183629-73183648 MsG0480022489.01.T01:CDS 55.0%
ACTGGCGTTGCCCACTGTTC+TGG - Chr4:73183622-73183641 MsG0480022489.01.T01:CDS 60.0%
! CGTTGCCCACTGTTCTGGCT+TGG - Chr4:73183627-73183646 MsG0480022489.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 73179822 73183727 73179822 ID=MsG0480022489.01;Name=MsG0480022489.01
Chr4 mRNA 73179822 73183727 73179822 ID=MsG0480022489.01.T01;Parent=MsG0480022489.01;Name=MsG0480022489.01.T01;_AED=0.49;_eAED=0.58;_QI=0|0.33|0.25|1|0.66|0.75|4|0|196
Chr4 exon 73183551 73183727 73183551 ID=MsG0480022489.01.T01:exon:2871;Parent=MsG0480022489.01.T01
Chr4 exon 73182667 73182829 73182667 ID=MsG0480022489.01.T01:exon:2870;Parent=MsG0480022489.01.T01
Chr4 exon 73182003 73182067 73182003 ID=MsG0480022489.01.T01:exon:2869;Parent=MsG0480022489.01.T01
Chr4 exon 73179822 73180007 73179822 ID=MsG0480022489.01.T01:exon:2868;Parent=MsG0480022489.01.T01
Chr4 CDS 73183551 73183727 73183551 ID=MsG0480022489.01.T01:cds;Parent=MsG0480022489.01.T01
Chr4 CDS 73182667 73182829 73182667 ID=MsG0480022489.01.T01:cds;Parent=MsG0480022489.01.T01
Chr4 CDS 73182003 73182067 73182003 ID=MsG0480022489.01.T01:cds;Parent=MsG0480022489.01.T01
Chr4 CDS 73179822 73180007 73179822 ID=MsG0480022489.01.T01:cds;Parent=MsG0480022489.01.T01
Gene Sequence

>MsG0480022489.01.T01

ATGCACTATGTGTATTGTTTATATATAGATCGTGCCAGAGAACTTGCTGATGTAATCGGTGGAGATGCTCTAACCCTCAGTGATTTAGATAATTACCATCCAGAGGATGGTATGATTCTAGCAAACACAACTTCAATCGGAATGCAACCAAAAGTTGATGAAACACCTGTTTCAAAGCATGCATTGAAATTTTACTCGTTGGTTTTTGATGCTGTCTACACGCCAAAAATGACTAGACTCTTGAAAGAAGCAGAAGAATCTGGAGCCACTATTGTAACAGGACTGGAGATGTTTATTGGACAAGCATATCGACAATATGAGCATTATACTGGATTGCCAGGAAATCGTGAAAATAATAGGAAAGTTGATTTCACAATTTTTTATACAAACTATTGGAAACAGGAGGATATTTTTTCCCACAAAGTTTCTAAAATGGTTCGGAACACACTCACGATGATAACGCTCTTAGTTCCACTGCTGCTATCACTGGCGTTGCCCACTGTTCTGGCTTGGGGAAAAGATGGTCACTATGCAATTTGTAAAATTTCACAGGTAACCCTAACCTTCTTCACTACACTACTCCTCACCTAA
Protein sequence

>MsG0480022489.01.T01

MHYVYCLYIDRARELADVIGGDALTLSDLDNYHPEDGMILANTTSIGMQPKVDETPVSKHALKFYSLVFDAVYTPKMTRLLKEAEESGATIVTGLEMFIGQAYRQYEHYTGLPGNRENNRKVDFTIFYTNYWKQEDIFSHKVSKMVRNTLTMITLLVPLLLSLALPTVLAWGKDGHYAICKISQVTLTFFTTLLLT*