AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022800.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022800.01.T01 MTR_4g096930 90.291 103 9 1 12 114 1 102 5.76e-51 176
MsG0480022800.01.T01 MTR_4g096930 94.595 74 4 0 117 190 171 244 1.80e-41 149
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022800.01.T01 AT5G44800 47.706 109 54 2 6 114 2 107 1.11e-22 95.1

Find 50 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAAATCTATTTCAACTA+TGG 0.261740 4:+76798155 MsG0480022800.01.T01:CDS
ACTATGGGAGGCAAATTCTT+TGG 0.270367 4:+76798171 MsG0480022800.01.T01:CDS
CAGCAACATCTGAATCTTCT+AGG 0.281233 4:+76795297 MsG0480022800.01.T01:CDS
AGGGAGAAAACTTCCTTATC+AGG 0.323098 4:-76798354 None:intergenic
CTACAATGCTTGACAAGAAC+TGG 0.348395 4:+76795189 MsG0480022800.01.T01:CDS
TGTCCATAGGTGAGACTGTT+GGG 0.354534 4:-76798317 None:intergenic
CAGTTCTTGTCAAGCATTGT+AGG 0.374231 4:-76795188 None:intergenic
CTAGAAGATTCAGATGTTGC+TGG 0.379549 4:-76795296 None:intergenic
GTGTCTTGACCCTCCTCTTA+AGG 0.405564 4:+76797819 MsG0480022800.01.T01:CDS
TCAAACAAACATACCTTAAG+AGG 0.432253 4:-76797832 None:intergenic
CTCTCAAGGGATGGATCCAT+TGG 0.438352 4:-76798240 None:intergenic
AGAGGACTGATCCGGTCCTA+TGG 0.442391 4:-76795240 None:intergenic
GATGTCAGTTTCACCTGATA+AGG 0.446516 4:+76798341 MsG0480022800.01.T01:CDS
CGCTACAAGTAGCATCCACT+GGG 0.448725 4:-76798212 None:intergenic
TAGTTGAAATAGATTTGCTT+TGG 0.468261 4:-76798152 None:intergenic
AGCAAATCTATTTCAACTAT+GGG 0.472649 4:+76798156 MsG0480022800.01.T01:CDS
TGGATTATCTTCTTTACCGT+TGG 0.479001 4:-76795276 None:intergenic
TCGCTACAAGTAGCATCCAC+TGG 0.501364 4:-76798213 None:intergenic
ATAGGACCGGATCAGTCCTC+TGG 0.509732 4:+76795242 MsG0480022800.01.T01:CDS
GAATGCGTGATCTGTGATCT+TGG 0.520786 4:+76797748 MsG0480022800.01.T01:CDS
TCCCTTGAGAGTTGCATGGA+AGG 0.525103 4:+76798252 MsG0480022800.01.T01:CDS
TTCCTTCCATGCAACTCTCA+AGG 0.529707 4:-76798254 None:intergenic
CGTATGTTAAAGACTGAAGA+AGG 0.530419 4:+76795335 MsG0480022800.01.T01:CDS
AGAAGAAGAAGGGACATGAC+GGG 0.533386 4:+76795378 MsG0480022800.01.T01:CDS
GTCCCCAACAGTCTCACCTA+TGG 0.539663 4:+76798314 MsG0480022800.01.T01:CDS
TCCTTCCATGCAACTCTCAA+GGG 0.541956 4:-76798253 None:intergenic
GCATCCACTGGGGAAAGCAC+TGG 0.545975 4:-76798201 None:intergenic
GAGGACTGATCCGGTCCTAT+GGG 0.552767 4:-76795239 None:intergenic
CAGGCACAAGTGAATGAAGA+AGG 0.556450 4:+76795148 None:intergenic
TCCATGCAACTCTCAAGGGA+TGG 0.564580 4:-76798249 None:intergenic
CAACAGTCTCACCTATGGAC+AGG 0.564975 4:+76798319 MsG0480022800.01.T01:CDS
TCTTTACCAGAGGACTGATC+CGG 0.566089 4:-76795248 None:intergenic
AACAAACATACCTTAAGAGG+AGG 0.573335 4:-76797829 None:intergenic
GAGAAAACTTCCCATAGGAC+CGG 0.577281 4:+76795229 MsG0480022800.01.T01:CDS
AAGAAGAAGAAGGGACATGA+CGG 0.578549 4:+76795377 MsG0480022800.01.T01:CDS
GAAACTGACATCCTGTCCAT+AGG 0.600018 4:-76798330 None:intergenic
TACAATGCTTGACAAGAACT+GGG 0.602956 4:+76795190 MsG0480022800.01.T01:CDS
CTGTCCATAGGTGAGACTGT+TGG 0.606864 4:-76798318 None:intergenic
TCCATCCCTTGAGAGTTGCA+TGG 0.610143 4:+76798248 MsG0480022800.01.T01:CDS
ACAAACATACCTTAAGAGGA+GGG 0.624531 4:-76797828 None:intergenic
TACTTGTAGCGACAAGCCAA+TGG 0.627602 4:+76798224 MsG0480022800.01.T01:CDS
AAATCTATTTCAACTATGGG+AGG 0.628489 4:+76798159 MsG0480022800.01.T01:CDS
GTTGGATTGCTCTTTACCAG+AGG 0.628893 4:-76795258 None:intergenic
GTCCATAGGTGAGACTGTTG+GGG 0.634809 4:-76798316 None:intergenic
GAAACCAGTGCTTTCCCCAG+TGG 0.637501 4:+76798197 MsG0480022800.01.T01:CDS
AAGCGGAGAAAACTTCCCAT+AGG 0.638970 4:+76795224 MsG0480022800.01.T01:CDS
TCTGGTAAAGAGCAATCCAA+CGG 0.684965 4:+76795260 MsG0480022800.01.T01:CDS
TGCGTGATCTGTGATCTTGG+TGG 0.690352 4:+76797751 MsG0480022800.01.T01:CDS
GAAGAAGAAGGGACATGACG+GGG 0.693634 4:+76795379 MsG0480022800.01.T01:CDS
GCTACAAGTAGCATCCACTG+GGG 0.832591 4:-76798211 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAACATATTTCAAAAAA+AGG + Chr4:76798125-76798144 MsG0480022800.01.T01:intron 10.0%
!! TCAACTATAATATTATTTAT+AGG + Chr4:76797617-76797636 MsG0480022800.01.T01:intron 10.0%
!!! TTGATATTATTTTTGAATTT+GGG + Chr4:76796925-76796944 MsG0480022800.01.T01:intron 10.0%
!!! TTTGATATTATTTTTGAATT+TGG + Chr4:76796924-76796943 MsG0480022800.01.T01:intron 10.0%
!! AAATTGAATCTAGATTATTT+AGG - Chr4:76798016-76798035 None:intergenic 15.0%
!! AATATTATAGTTGAAAAAGA+TGG - Chr4:76797611-76797630 None:intergenic 15.0%
!! AATTGAATCTAGATTATTTA+GGG - Chr4:76798015-76798034 None:intergenic 15.0%
!! ATATGATATATCTTACAATT+TGG + Chr4:76796713-76796732 MsG0480022800.01.T01:intron 15.0%
!!! TTGATACTTTTTAATTCTAT+AGG + Chr4:76797122-76797141 MsG0480022800.01.T01:intron 15.0%
!!! TTTTTACATTTCCATATATT+TGG + Chr4:76795507-76795526 MsG0480022800.01.T01:intron 15.0%
!! ACATCAATAACCATAATTAT+AGG - Chr4:76797066-76797085 None:intergenic 20.0%
!! ATATGACAAACAAAATAAAG+AGG - Chr4:76797544-76797563 None:intergenic 20.0%
!! ATTTATAGGAAAATCTTACT+GGG + Chr4:76797631-76797650 MsG0480022800.01.T01:intron 20.0%
!! CCAAAAATATATGTATAAGT+GGG - Chr4:76796788-76796807 None:intergenic 20.0%
!! TAAACAAGTAAACTAATATC+AGG - Chr4:76797412-76797431 None:intergenic 20.0%
!! TAAATCTTCTATATGATTTC+AGG - Chr4:76796638-76796657 None:intergenic 20.0%
!! TATACGATTACCTATAATTA+TGG + Chr4:76797053-76797072 MsG0480022800.01.T01:intron 20.0%
!! TATTTATAGGAAAATCTTAC+TGG + Chr4:76797630-76797649 MsG0480022800.01.T01:intron 20.0%
!! TTAAATGTTCTGATACAAAT+GGG + Chr4:76797159-76797178 MsG0480022800.01.T01:intron 20.0%
!! TTTAAATGTTCTGATACAAA+TGG + Chr4:76797158-76797177 MsG0480022800.01.T01:intron 20.0%
!!! ATTATTTTTGAATTTGGGTT+TGG + Chr4:76796930-76796949 MsG0480022800.01.T01:intron 20.0%
!!! CCACTTATACATATATTTTT+GGG + Chr4:76796786-76796805 MsG0480022800.01.T01:intron 20.0%
! AAAATATATGTATAAGTGGG+AGG - Chr4:76796785-76796804 None:intergenic 25.0%
! AAGCAAATCTATTTCAACTA+TGG + Chr4:76798155-76798174 MsG0480022800.01.T01:CDS 25.0%
! AGATGGAATTATGAATACAT+AGG - Chr4:76797594-76797613 None:intergenic 25.0%
! AGCAAATCTATTTCAACTAT+GGG + Chr4:76798156-76798175 MsG0480022800.01.T01:CDS 25.0%
! AGTCGAACATTTATAAAGAA+GGG + Chr4:76795449-76795468 MsG0480022800.01.T01:intron 25.0%
! AGTGAAGTAATCCAAATATA+TGG - Chr4:76795521-76795540 None:intergenic 25.0%
! ATCATTATTGTTACCTTTAG+AGG + Chr4:76797432-76797451 MsG0480022800.01.T01:intron 25.0%
! ATGCTGAATTATGTATATCT+AGG - Chr4:76795838-76795857 None:intergenic 25.0%
! ATGCTTCAGATATACAATTT+AGG - Chr4:76796857-76796876 None:intergenic 25.0%
! ATGGTCGATTAACATAATAA+CGG + Chr4:76795716-76795735 MsG0480022800.01.T01:intron 25.0%
! ATTAAAAAACCATGATTCTC+AGG + Chr4:76797885-76797904 MsG0480022800.01.T01:intron 25.0%
! ATTTAGCAAGTGTAAATACA+TGG + Chr4:76795697-76795716 MsG0480022800.01.T01:intron 25.0%
! CCCAAAAATATATGTATAAG+TGG - Chr4:76796789-76796808 None:intergenic 25.0%
! GTATTGTGTATGCATTTATT+TGG + Chr4:76797239-76797258 MsG0480022800.01.T01:intron 25.0%
! TTGTATATAAGTCAATGATC+AGG + Chr4:76795742-76795761 MsG0480022800.01.T01:intron 25.0%
! TTTATAGGAAAATCTTACTG+GGG + Chr4:76797632-76797651 MsG0480022800.01.T01:intron 25.0%
!! ATTTTCATCTAAGAAGAAGA+AGG + Chr4:76795367-76795386 MsG0480022800.01.T01:CDS 25.0%
!! CCCACTTATACATATATTTT+TGG + Chr4:76796785-76796804 MsG0480022800.01.T01:intron 25.0%
!! TAGTTGAAATAGATTTGCTT+TGG - Chr4:76798155-76798174 None:intergenic 25.0%
!! TTTTCATCTAAGAAGAAGAA+GGG + Chr4:76795368-76795387 MsG0480022800.01.T01:CDS 25.0%
!!! AATAGAAGTTCTAGATTATG+TGG - Chr4:76795945-76795964 None:intergenic 25.0%
!!! ATAGAAGTTCTAGATTATGT+GGG - Chr4:76795944-76795963 None:intergenic 25.0%
!!! ATGCATTTATTTGGTGTTAA+TGG + Chr4:76797248-76797267 MsG0480022800.01.T01:intron 25.0%
!!! ATTTATTTGGTGTTAATGGT+AGG + Chr4:76797252-76797271 MsG0480022800.01.T01:intron 25.0%
!!! TGGCTAATGAGATATTTTTA+GGG + Chr4:76797356-76797375 MsG0480022800.01.T01:intron 25.0%
!!! TTTTCTAGCAAAATTCTTCT+GGG + Chr4:76797473-76797492 MsG0480022800.01.T01:intron 25.0%
AAATACCAAGAGAGAAAAAC+AGG - Chr4:76796067-76796086 None:intergenic 30.0%
AAATCTATTTCAACTATGGG+AGG + Chr4:76798159-76798178 MsG0480022800.01.T01:CDS 30.0%
AAGCTACCTCAAAAGAATAA+TGG + Chr4:76796586-76796605 MsG0480022800.01.T01:intron 30.0%
ACTATCCATAAGCAAATGAT+TGG - Chr4:76795422-76795441 None:intergenic 30.0%
ATCTTCTATATGATTTCAGG+TGG - Chr4:76796635-76796654 None:intergenic 30.0%
ATTTATAAAGAAGGGCCAAT+AGG + Chr4:76795457-76795476 MsG0480022800.01.T01:intron 30.0%
CAGTCGAACATTTATAAAGA+AGG + Chr4:76795448-76795467 MsG0480022800.01.T01:intron 30.0%
CTAAAAATATCTCATTAGCC+AGG - Chr4:76797357-76797376 None:intergenic 30.0%
CTAATGATTAGCACATTTAC+TGG + Chr4:76795776-76795795 MsG0480022800.01.T01:intron 30.0%
CTTTATAAATGTTCGACTGA+AGG - Chr4:76795447-76795466 None:intergenic 30.0%
TACTCTCCCTCATATTTATA+CGG + Chr4:76795857-76795876 MsG0480022800.01.T01:intron 30.0%
TATTTCGAAAAGAATTGCCT+TGG + Chr4:76797320-76797339 MsG0480022800.01.T01:intron 30.0%
TCAAACAAACATACCTTAAG+AGG - Chr4:76797835-76797854 None:intergenic 30.0%
TCAATGAGTAGAAGAACAAT+GGG - Chr4:76796384-76796403 None:intergenic 30.0%
TGACAAATGATACTTTGAGT+TGG + Chr4:76796401-76796420 MsG0480022800.01.T01:intron 30.0%
TTCAGACCGTATAAATATGA+GGG - Chr4:76795866-76795885 None:intergenic 30.0%
! CTGGCTAATGAGATATTTTT+AGG + Chr4:76797355-76797374 MsG0480022800.01.T01:intron 30.0%
! TATACAATTTAGGACGTGAT+AGG - Chr4:76796847-76796866 None:intergenic 30.0%
! TGTTGATAGATTTGTGTCTT+TGG + Chr4:76795540-76795559 MsG0480022800.01.T01:intron 30.0%
! TTACCTTTAGAGGATATGTT+GGG + Chr4:76797442-76797461 MsG0480022800.01.T01:intron 30.0%
!! ATTCTCAGGTTGTGTATTTT+TGG + Chr4:76797899-76797918 MsG0480022800.01.T01:intron 30.0%
!! CAAGTTCAAGTTCTTTTCAT+CGG - Chr4:76798295-76798314 None:intergenic 30.0%
!! CTTTTCTAGCAAAATTCTTC+TGG + Chr4:76797472-76797491 MsG0480022800.01.T01:intron 30.0%
!!! AGCTTTTGATTTTACAGGTA+CGG - Chr4:76798060-76798079 None:intergenic 30.0%
!!! AGGGGTTTTTTTTATCTAAG+GGG + Chr4:76795623-76795642 MsG0480022800.01.T01:intron 30.0%
!!! GAGGGGTTTTTTTTATCTAA+GGG + Chr4:76795622-76795641 MsG0480022800.01.T01:intron 30.0%
!!! TGAGGGGTTTTTTTTATCTA+AGG + Chr4:76795621-76795640 MsG0480022800.01.T01:intron 30.0%
AACAAACATACCTTAAGAGG+AGG - Chr4:76797832-76797851 None:intergenic 35.0%
AAGCTTATCCAAGATCTGTT+AGG - Chr4:76796906-76796925 None:intergenic 35.0%
AATACACAACCTGAGAATCA+TGG - Chr4:76797897-76797916 None:intergenic 35.0%
ACAAACATACCTTAAGAGGA+GGG - Chr4:76797831-76797850 None:intergenic 35.0%
ACTGGCATGTGAAGAAAAAT+CGG - Chr4:76796218-76796237 None:intergenic 35.0%
AGTTACAACCATGCAAAAAG+TGG - Chr4:76797187-76797206 None:intergenic 35.0%
ATACTCGCGTTGTATTCAAT+TGG + Chr4:76795911-76795930 MsG0480022800.01.T01:intron 35.0%
CAGCTTTCTAGATTCTATCA+AGG - Chr4:76796612-76796631 None:intergenic 35.0%
CAGTTATATCTGCTTCTGTT+TGG + Chr4:76796008-76796027 MsG0480022800.01.T01:intron 35.0%
CGTATGTTAAAGACTGAAGA+AGG + Chr4:76795335-76795354 MsG0480022800.01.T01:CDS 35.0%
CTATGAGTAAAATCAACCTG+AGG - Chr4:76796681-76796700 None:intergenic 35.0%
CTTCAGACCGTATAAATATG+AGG - Chr4:76795867-76795886 None:intergenic 35.0%
GTACCTGTAAAATCAAAAGC+TGG + Chr4:76798059-76798078 MsG0480022800.01.T01:intron 35.0%
GTCAATGAGTAGAAGAACAA+TGG - Chr4:76796385-76796404 None:intergenic 35.0%
TACAATGCTTGACAAGAACT+GGG + Chr4:76795190-76795209 MsG0480022800.01.T01:CDS 35.0%
TAGGCTCAAATAATTTCGTG+AGG - Chr4:76796105-76796124 None:intergenic 35.0%
TATCAATCAAGCAGCTGATT+GGG - Chr4:76797108-76797127 None:intergenic 35.0%
TCACGCATTCATAGAAATAC+TGG - Chr4:76797738-76797757 None:intergenic 35.0%
TGGATTATCTTCTTTACCGT+TGG - Chr4:76795279-76795298 None:intergenic 35.0%
TGTGTCCAATCATTTGCTTA+TGG + Chr4:76795414-76795433 MsG0480022800.01.T01:intron 35.0%
TTAAAGCCTCTTGTCATTGT+TGG - Chr4:76796538-76796557 None:intergenic 35.0%
TTATTACCAAGTCCCTTAGA+GGG + Chr4:76796655-76796674 MsG0480022800.01.T01:intron 35.0%
TTTATTACCAAGTCCCTTAG+AGG + Chr4:76796654-76796673 MsG0480022800.01.T01:intron 35.0%
! AATATATGCGATCTAGCTTG+AGG + Chr4:76796998-76797017 MsG0480022800.01.T01:intron 35.0%
! ACTTGCAGTACTTTTCCTAT+TGG - Chr4:76795475-76795494 None:intergenic 35.0%
! ATGACAAGAGGCTTTAATGA+CGG + Chr4:76796541-76796560 MsG0480022800.01.T01:intron 35.0%
! CATGATGATTCTTTTCCAGT+TGG + Chr4:76795980-76795999 MsG0480022800.01.T01:intron 35.0%
! CTTTTTGCATGGTTGTAACT+TGG + Chr4:76797187-76797206 MsG0480022800.01.T01:intron 35.0%
! GTTACCTTTAGAGGATATGT+TGG + Chr4:76797441-76797460 MsG0480022800.01.T01:intron 35.0%
! TCAAGGCCATTATTCTTTTG+AGG - Chr4:76796595-76796614 None:intergenic 35.0%
! TCATTTTCTTCAAAGCAGCT+TGG - Chr4:76797982-76798001 None:intergenic 35.0%
!! AAATTCTTCTGGGACATACA+CGG + Chr4:76797483-76797502 MsG0480022800.01.T01:intron 35.0%
!! AAGAATTGCCTTGGCTTTTA+TGG + Chr4:76797329-76797348 MsG0480022800.01.T01:intron 35.0%
!! CAAATGATACTTTGAGTTGG+AGG + Chr4:76796404-76796423 MsG0480022800.01.T01:intron 35.0%
!! GTTAAGTGCTGACTAATTTG+AGG - Chr4:76796177-76796196 None:intergenic 35.0%
!! TTAAGCCTGTTTTTCTCTCT+TGG + Chr4:76796059-76796078 MsG0480022800.01.T01:intron 35.0%
!!! ACTCCAGCTTTTGATTTTAC+AGG - Chr4:76798065-76798084 None:intergenic 35.0%
!!! ATGTCATTTTCACTGTTGAG+GGG + Chr4:76795605-76795624 MsG0480022800.01.T01:intron 35.0%
!!! CATGTCATTTTCACTGTTGA+GGG + Chr4:76795604-76795623 MsG0480022800.01.T01:intron 35.0%
!!! GAAAAGGTTTCTGGGATTTT+CGG + Chr4:76798101-76798120 MsG0480022800.01.T01:intron 35.0%
!!! GGGGTTTTTTTTATCTAAGG+GGG + Chr4:76795624-76795643 MsG0480022800.01.T01:intron 35.0%
!!! TCATGTCATTTTCACTGTTG+AGG + Chr4:76795603-76795622 MsG0480022800.01.T01:intron 35.0%
!!! TTCTTTTGAGGTAGCTTTAG+TGG - Chr4:76796583-76796602 None:intergenic 35.0%
AAGAAGAAGAAGGGACATGA+CGG + Chr4:76795377-76795396 MsG0480022800.01.T01:CDS 40.0%
AAGTCTATTGAGAAACTGGC+CGG - Chr4:76796299-76796318 None:intergenic 40.0%
ACACTCCTATCTGTCTTGTA+AGG + Chr4:76796962-76796981 MsG0480022800.01.T01:intron 40.0%
ACTATGGGAGGCAAATTCTT+TGG + Chr4:76798171-76798190 MsG0480022800.01.T01:CDS 40.0%
AGCAAGAAATCATGGTGTGT+AGG - Chr4:76796124-76796143 None:intergenic 40.0%
AGGGAGAAAACTTCCTTATC+AGG - Chr4:76798357-76798376 None:intergenic 40.0%
ATTCAATTTCAAGACGAGCG+AGG + Chr4:76798027-76798046 MsG0480022800.01.T01:intron 40.0%
ATTGTTGGCAGGGGAATAAA+TGG - Chr4:76796523-76796542 None:intergenic 40.0%
CAGCAACATCTGAATCTTCT+AGG + Chr4:76795297-76795316 MsG0480022800.01.T01:CDS 40.0%
CTACAATGCTTGACAAGAAC+TGG + Chr4:76795189-76795208 MsG0480022800.01.T01:CDS 40.0%
CTAGAAGATTCAGATGTTGC+TGG - Chr4:76795299-76795318 None:intergenic 40.0%
GAAAACAAGCCCGCTAATAT+CGG - Chr4:76797390-76797409 None:intergenic 40.0%
GAGCAAGTCTATTGAGAAAC+TGG - Chr4:76796303-76796322 None:intergenic 40.0%
GATGTCAGTTTCACCTGATA+AGG + Chr4:76798341-76798360 MsG0480022800.01.T01:CDS 40.0%
GCACTTAACATTGCTGCTAA+TGG + Chr4:76796188-76796207 MsG0480022800.01.T01:intron 40.0%
GCTCTGCAGTATGATTCATA+TGG + Chr4:76797212-76797231 MsG0480022800.01.T01:intron 40.0%
GGTTCTATGTCCGATATTAG+CGG + Chr4:76797377-76797396 MsG0480022800.01.T01:intron 40.0%
GTATCAATCAAGCAGCTGAT+TGG - Chr4:76797109-76797128 None:intergenic 40.0%
GTTCCCAACATATCCTCTAA+AGG - Chr4:76797448-76797467 None:intergenic 40.0%
GTTCTATGTCCGATATTAGC+GGG + Chr4:76797378-76797397 MsG0480022800.01.T01:intron 40.0%
TATAGCCAGATTTCAGCAAC+TGG - Chr4:76796236-76796255 None:intergenic 40.0%
TCAACCTGGAAAAGGTTTCT+GGG + Chr4:76798093-76798112 MsG0480022800.01.T01:intron 40.0%
TCATCATGAGATAGACACTG+TGG - Chr4:76795968-76795987 None:intergenic 40.0%
TCTCACCTTACAAGACAGAT+AGG - Chr4:76796970-76796989 None:intergenic 40.0%
TCTGAGCAAGCAAGAAATCA+TGG - Chr4:76796132-76796151 None:intergenic 40.0%
TCTGGTAAAGAGCAATCCAA+CGG + Chr4:76795260-76795279 MsG0480022800.01.T01:CDS 40.0%
TGAGTAGAAGAACAATGGGA+AGG - Chr4:76796380-76796399 None:intergenic 40.0%
TTGCTGTCATTGTTCAGCAA+AGG - Chr4:76796460-76796479 None:intergenic 40.0%
TTGTTGGCAGGGGAATAAAT+GGG - Chr4:76796522-76796541 None:intergenic 40.0%
! AAAACCGACTTATAAGGCGA+GGG + Chr4:76796757-76796776 MsG0480022800.01.T01:intron 40.0%
! AAATCCCAGAAACCTTTTCC+AGG - Chr4:76798100-76798119 None:intergenic 40.0%
! AGTGTGTGAGTTACTTTCTC+CGG + Chr4:76796277-76796296 MsG0480022800.01.T01:intron 40.0%
! ATGATTCTTTTCCAGTTGGC+CGG + Chr4:76795984-76796003 MsG0480022800.01.T01:intron 40.0%
! CAGTTCTTGTCAAGCATTGT+AGG - Chr4:76795191-76795210 None:intergenic 40.0%
! CCACACAAAACCGACTTATA+AGG + Chr4:76796751-76796770 MsG0480022800.01.T01:intron 40.0%
! GGGAATAAATGGGCTTCTTA+CGG - Chr4:76796512-76796531 None:intergenic 40.0%
! TATCTGAAGCATGATCCAAC+TGG + Chr4:76796865-76796884 MsG0480022800.01.T01:intron 40.0%
!! AAACCTTTTCCAGGTTGACA+GGG - Chr4:76798091-76798110 None:intergenic 40.0%
!! AATTCTTCTGGGACATACAC+GGG + Chr4:76797484-76797503 MsG0480022800.01.T01:intron 40.0%
!! ATACTTTGAGTTGGAGGCAA+TGG + Chr4:76796410-76796429 MsG0480022800.01.T01:intron 40.0%
!! CCTTATAAGTCGGTTTTGTG+TGG - Chr4:76796754-76796773 None:intergenic 40.0%
!! CGGTTTTGTGTGGTTGAATT+AGG - Chr4:76796744-76796763 None:intergenic 40.0%
!!! TTTTGAGGTAGCTTTAGTGG+TGG - Chr4:76796580-76796599 None:intergenic 40.0%
AAGCGGAGAAAACTTCCCAT+AGG + Chr4:76795224-76795243 MsG0480022800.01.T01:CDS 45.0%
ACATGCCAGTTGCTGAAATC+TGG + Chr4:76796228-76796247 MsG0480022800.01.T01:intron 45.0%
AGAAGAAGAAGGGACATGAC+GGG + Chr4:76795378-76795397 MsG0480022800.01.T01:CDS 45.0%
CAGAAGCAGATATAACTGCC+CGG - Chr4:76796006-76796025 None:intergenic 45.0%
CGCGTTGTATTCAATTGGCT+GGG + Chr4:76795916-76795935 MsG0480022800.01.T01:intron 45.0%
CTAGCAGAGACAGTGCTTTA+CGG - Chr4:76796329-76796348 None:intergenic 45.0%
GAAACTGACATCCTGTCCAT+AGG - Chr4:76798333-76798352 None:intergenic 45.0%
GAATGCGTGATCTGTGATCT+TGG + Chr4:76797748-76797767 MsG0480022800.01.T01:CDS 45.0%
GACATACACGGGAGTAGATT+TGG + Chr4:76797495-76797514 MsG0480022800.01.T01:intron 45.0%
GAGAAAACTTCCCATAGGAC+CGG + Chr4:76795229-76795248 MsG0480022800.01.T01:CDS 45.0%
GGCATGTGAAGAAAAATCGG+AGG - Chr4:76796215-76796234 None:intergenic 45.0%
GGCCATATGTCATCCAATGT+GGG + Chr4:76796807-76796826 MsG0480022800.01.T01:intron 45.0%
GTCAACCTGGAAAAGGTTTC+TGG + Chr4:76798092-76798111 MsG0480022800.01.T01:intron 45.0%
GTCCCACATTGGATGACATA+TGG - Chr4:76796812-76796831 None:intergenic 45.0%
GTCTTGTAAGGTGAGACTCT+CGG + Chr4:76796974-76796993 MsG0480022800.01.T01:intron 45.0%
GTGTGTTAAGAGTCCCACAT+TGG - Chr4:76796823-76796842 None:intergenic 45.0%
GTTGGATTGCTCTTTACCAG+AGG - Chr4:76795261-76795280 None:intergenic 45.0%
TAACCCTGTCAACCTGGAAA+AGG + Chr4:76798085-76798104 MsG0480022800.01.T01:intron 45.0%
TACAACGCGAGTATGTCAAG+AGG - Chr4:76795905-76795924 None:intergenic 45.0%
TACTTGTAGCGACAAGCCAA+TGG + Chr4:76798224-76798243 MsG0480022800.01.T01:CDS 45.0%
TCCTTCCATGCAACTCTCAA+GGG - Chr4:76798256-76798275 None:intergenic 45.0%
TCGCGTTGTATTCAATTGGC+TGG + Chr4:76795915-76795934 MsG0480022800.01.T01:intron 45.0%
TCTTTACCAGAGGACTGATC+CGG - Chr4:76795251-76795270 None:intergenic 45.0%
TGAGGCAGTCAAACACAGTT+GGG - Chr4:76796159-76796178 None:intergenic 45.0%
TGTCCATAGGTGAGACTGTT+GGG - Chr4:76798320-76798339 None:intergenic 45.0%
TTCCTTCCATGCAACTCTCA+AGG - Chr4:76798257-76798276 None:intergenic 45.0%
TTGAGGCAGTCAAACACAGT+TGG - Chr4:76796160-76796179 None:intergenic 45.0%
! CAAAACCGACTTATAAGGCG+AGG + Chr4:76796756-76796775 MsG0480022800.01.T01:intron 45.0%
! TGATTCTTTTCCAGTTGGCC+GGG + Chr4:76795985-76796004 MsG0480022800.01.T01:intron 45.0%
!! GAAACCTTTTCCAGGTTGAC+AGG - Chr4:76798092-76798111 None:intergenic 45.0%
!!! AATGGGCACCACTTTTTGCA+TGG + Chr4:76797176-76797195 MsG0480022800.01.T01:intron 45.0%
!!! ACTGGGTTTTAAAGCGCAAG+CGG + Chr4:76795207-76795226 MsG0480022800.01.T01:CDS 45.0%
AATCAACCTGAGGCCCTCTA+AGG - Chr4:76796671-76796690 None:intergenic 50.0%
ACGCGAGTATGTCAAGAGGA+AGG - Chr4:76795901-76795920 None:intergenic 50.0%
AGCCTCTTGTCATTGTTGGC+AGG - Chr4:76796534-76796553 None:intergenic 50.0%
ATCAACCTGAGGCCCTCTAA+GGG - Chr4:76796670-76796689 None:intergenic 50.0%
CAACAGTCTCACCTATGGAC+AGG + Chr4:76798319-76798338 MsG0480022800.01.T01:CDS 50.0%
CCTCTTGTCATTGTTGGCAG+GGG - Chr4:76796532-76796551 None:intergenic 50.0%
CGCTACAAGTAGCATCCACT+GGG - Chr4:76798215-76798234 None:intergenic 50.0%
CTCTCAAGGGATGGATCCAT+TGG - Chr4:76798243-76798262 None:intergenic 50.0%
CTGTCCATAGGTGAGACTGT+TGG - Chr4:76798321-76798340 None:intergenic 50.0%
GAAGAAGAAGGGACATGACG+GGG + Chr4:76795379-76795398 MsG0480022800.01.T01:CDS 50.0%
GCCTCTTGTCATTGTTGGCA+GGG - Chr4:76796533-76796552 None:intergenic 50.0%
GCTACAAGTAGCATCCACTG+GGG - Chr4:76798214-76798233 None:intergenic 50.0%
GGGCCATATGTCATCCAATG+TGG + Chr4:76796806-76796825 MsG0480022800.01.T01:intron 50.0%
GTCCATAGGTGAGACTGTTG+GGG - Chr4:76798319-76798338 None:intergenic 50.0%
GTGTCTTGACCCTCCTCTTA+AGG + Chr4:76797819-76797838 MsG0480022800.01.T01:CDS 50.0%
TCCATCCCTTGAGAGTTGCA+TGG + Chr4:76798248-76798267 MsG0480022800.01.T01:CDS 50.0%
TCCATGCAACTCTCAAGGGA+TGG - Chr4:76798252-76798271 None:intergenic 50.0%
TCCCTTGAGAGTTGCATGGA+AGG + Chr4:76798252-76798271 MsG0480022800.01.T01:CDS 50.0%
TCGCTACAAGTAGCATCCAC+TGG - Chr4:76798216-76798235 None:intergenic 50.0%
TGCGTGATCTGTGATCTTGG+TGG + Chr4:76797751-76797770 MsG0480022800.01.T01:CDS 50.0%
TGGAGTTAACCCTGTCAACC+TGG + Chr4:76798079-76798098 MsG0480022800.01.T01:intron 50.0%
! GACATTGCCACTTCCCCATA+GGG - Chr4:76797960-76797979 None:intergenic 50.0%
AGAGGACTGATCCGGTCCTA+TGG - Chr4:76795243-76795262 None:intergenic 55.0%
ATAGGACCGGATCAGTCCTC+TGG + Chr4:76795242-76795261 MsG0480022800.01.T01:CDS 55.0%
CCCCTGCCAACAATGACAAG+AGG + Chr4:76796529-76796548 MsG0480022800.01.T01:intron 55.0%
CTCCTGAAGCGCATCCCTAT+GGG + Chr4:76797943-76797962 MsG0480022800.01.T01:intron 55.0%
GAAACCAGTGCTTTCCCCAG+TGG + Chr4:76798197-76798216 MsG0480022800.01.T01:CDS 55.0%
GAGGACTGATCCGGTCCTAT+GGG - Chr4:76795242-76795261 None:intergenic 55.0%
GATATAACTGCCCGGCCAAC+TGG - Chr4:76795998-76796017 None:intergenic 55.0%
GCCAGGCACCATAAAAGCCA+AGG - Chr4:76797340-76797359 None:intergenic 55.0%
GGCACCCTCGCCTTATAAGT+CGG - Chr4:76796764-76796783 None:intergenic 55.0%
GTCCCCAACAGTCTCACCTA+TGG + Chr4:76798314-76798333 MsG0480022800.01.T01:CDS 55.0%
TCCTGAAGCGCATCCCTATG+GGG + Chr4:76797944-76797963 MsG0480022800.01.T01:intron 55.0%
TCTCCTGAAGCGCATCCCTA+TGG + Chr4:76797942-76797961 MsG0480022800.01.T01:intron 55.0%
TGATCCAACTGGCCCGATAG+CGG + Chr4:76796876-76796895 MsG0480022800.01.T01:intron 55.0%
! GGACATTGCCACTTCCCCAT+AGG - Chr4:76797961-76797980 None:intergenic 55.0%
!! GAGGTAGCTTTAGTGGTGGC+TGG - Chr4:76796576-76796595 None:intergenic 55.0%
!! GCCTTGGCTTTTATGGTGCC+TGG + Chr4:76797336-76797355 MsG0480022800.01.T01:intron 55.0%
!! TAGCTTTAGTGGTGGCTGGC+AGG - Chr4:76796572-76796591 None:intergenic 55.0%
AGCGCATCCCTATGGGGAAG+TGG + Chr4:76797950-76797969 MsG0480022800.01.T01:intron 60.0%
CAAGTCCCTTAGAGGGCCTC+AGG + Chr4:76796662-76796681 MsG0480022800.01.T01:intron 60.0%
GATCCAACTGGCCCGATAGC+GGG + Chr4:76796877-76796896 MsG0480022800.01.T01:intron 60.0%
TCCCCATAGGGATGCGCTTC+AGG - Chr4:76797948-76797967 None:intergenic 60.0%
TCTGTTAGGCCGCCCGCTAT+CGG - Chr4:76796892-76796911 None:intergenic 60.0%
! CTGAGGCCCTCTAAGGGACT+TGG - Chr4:76796664-76796683 None:intergenic 60.0%
! GCATCCACTGGGGAAAGCAC+TGG - Chr4:76798204-76798223 None:intergenic 60.0%
CTGTTAGGCCGCCCGCTATC+GGG - Chr4:76796891-76796910 None:intergenic 65.0%
GCGGGCGGCCTAACAGATCT+TGG + Chr4:76796895-76796914 MsG0480022800.01.T01:intron 65.0%
!! CCAACTGGCCCGATAGCGGG+CGG + Chr4:76796880-76796899 MsG0480022800.01.T01:intron 70.0%
CCGCCCGCTATCGGGCCAGT+TGG - Chr4:76796883-76796902 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 76795161 76798380 76795161 ID=MsG0480022800.01;Name=MsG0480022800.01
Chr4 mRNA 76795161 76798380 76795161 ID=MsG0480022800.01.T01;Parent=MsG0480022800.01;Name=MsG0480022800.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|191
Chr4 exon 76795161 76795400 76795161 ID=MsG0480022800.01.T01:exon:19829;Parent=MsG0480022800.01.T01
Chr4 exon 76797739 76797840 76797739 ID=MsG0480022800.01.T01:exon:19830;Parent=MsG0480022800.01.T01
Chr4 exon 76798147 76798380 76798147 ID=MsG0480022800.01.T01:exon:19831;Parent=MsG0480022800.01.T01
Chr4 CDS 76795161 76795400 76795161 ID=MsG0480022800.01.T01:cds;Parent=MsG0480022800.01.T01
Chr4 CDS 76797739 76797840 76797739 ID=MsG0480022800.01.T01:cds;Parent=MsG0480022800.01.T01
Chr4 CDS 76798147 76798380 76798147 ID=MsG0480022800.01.T01:cds;Parent=MsG0480022800.01.T01
Gene Sequence

>MsG0480022800.01.T01

ATGAAGAAGGAAAGCAAATCATCTGCTCCTACAATGCTTGACAAGAACTGGGTTTTAAAGCGCAAGCGGAGAAAACTTCCCATAGGACCGGATCAGTCCTCTGGTAAAGAGCAATCCAACGGTAAAGAAGATAATCCAGCAACATCTGAATCTTCTAGGAGTGCTTCTGCTAAACGTATGTTAAAGACTGAAGAAGGCACTGCTCAATTTTCATCTAAGAAGAAGAAGGGACATGACGGGTATTTCTATGAATGCGTGATCTGTGATCTTGGTGGCAACTTATTGTGTGCTGTGATAGCTTCTCGCACTTATCATTTACAGTGTCTTGACCCTCCTCTTAAGGTCAACCAAAGCAAATCTATTTCAACTATGGGAGGCAAATTCTTTGGAATGAAACCAGTGCTTTCCCCAGTGGATGCTACTTGTAGCGACAAGCCAATGGATCCATCCCTTGAGAGTTGCATGGAAGGAACTTCATGTGCAGATGCCGATGAAAAGAACTTGAACTTGTCCCCAACAGTCTCACCTATGGACAGGATGTCAGTTTCACCTGATAAGGAAGTTTTCTCCCTCTAA
Protein sequence

>MsG0480022800.01.T01

MKKESKSSAPTMLDKNWVLKRKRRKLPIGPDQSSGKEQSNGKEDNPATSESSRSASAKRMLKTEEGTAQFSSKKKKGHDGYFYECVICDLGGNLLCAVIASRTYHLQCLDPPLKVNQSKSISTMGGKFFGMKPVLSPVDATCSDKPMDPSLESCMEGTSCADADEKNLNLSPTVSPMDRMSVSPDKEVFSL*