AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022818.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022818.01.T01 MTR_4g094708 98.466 326 5 0 1 326 357 682 0.0 661
MsG0480022818.01.T01 MTR_4g094705 90.881 329 30 0 1 329 358 686 0.0 608
MsG0480022818.01.T01 MTR_5g066710 43.000 300 147 5 19 314 422 701 1.20e-63 218
MsG0480022818.01.T01 MTR_3g099777 42.000 300 150 5 19 314 444 723 5.73e-62 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022818.01.T01 AT1G55860 73.065 323 75 2 1 323 352 662 4.06e-142 444
MsG0480022818.01.T01 AT1G70320 72.840 324 76 3 1 323 352 664 7.73e-141 441

Find 63 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGCATTGTTCAGAGATT+TGG 0.223543 4:+77062365 MsG0480022818.01.T01:CDS
TTCAGGCGAGCAAAAGATTT+TGG 0.223733 4:+77062684 MsG0480022818.01.T01:CDS
TCTGGAAACCATCTCAAAAT+TGG 0.233242 4:+77063079 MsG0480022818.01.T01:CDS
ACATATTTGCGTGCTCTTAC+AGG 0.245479 4:+77062963 MsG0480022818.01.T01:CDS
GGATATGTTAGTTGAGATTC+TGG 0.281985 4:+77063061 MsG0480022818.01.T01:CDS
CTCCGGTGATAAGAAATTAA+AGG 0.327958 4:-77062546 None:intergenic
ATCAATTATAGCATCCAAAA+AGG 0.328954 4:-77062805 None:intergenic
GGAAATACTACTCGTATATA+TGG 0.330033 4:+77062621 MsG0480022818.01.T01:CDS
GCTGCATTGTTCAGAGATTT+GGG 0.343483 4:+77062366 MsG0480022818.01.T01:CDS
CTTAAACAGTAACGGCCTTC+AGG 0.366391 4:+77062887 MsG0480022818.01.T01:CDS
GCTGCTTCGTTACGTGGGCC+TGG 0.394997 4:+77063035 MsG0480022818.01.T01:CDS
GAAGTCTCCCATGTTGAAAA+TGG 0.400067 4:+77062423 MsG0480022818.01.T01:CDS
TTTATCTCTTGTAACTGTGT+TGG 0.400347 4:+77062191 MsG0480022818.01.T01:CDS
AGTCCAGCTGCGTCTAAAAC+GGG 0.402917 4:-77062774 None:intergenic
TATATACGAGTAGTATTTCC+AGG 0.405152 4:-77062618 None:intergenic
TGGTTGGAGGTTCTTCAGTT+TGG 0.409660 4:+77062493 MsG0480022818.01.T01:CDS
TTATTATTGATACATCAAAA+TGG 0.423114 4:+77062145 MsG0480022818.01.T01:CDS
AAGAGCACGCAAATATGTTC+TGG 0.438853 4:-77062958 None:intergenic
GTCTAGTAGAAGTGCACATA+TGG 0.442558 4:+77062473 MsG0480022818.01.T01:CDS
AACCTTTAATTTCTTATCAC+CGG 0.448588 4:+77062544 MsG0480022818.01.T01:CDS
TTATTGCGTGCTATATCCCT+TGG 0.453155 4:+77062585 MsG0480022818.01.T01:CDS
TCTCTGAACAATGCAGCAGC+TGG 0.453969 4:-77062360 None:intergenic
AAGTCCAGCTGCGTCTAAAA+CGG 0.455690 4:-77062775 None:intergenic
TGAAAGATAGAAATTCTTTG+AGG 0.457778 4:+77062913 MsG0480022818.01.T01:CDS
CATATTTGCGTGCTCTTACA+GGG 0.460381 4:+77062964 MsG0480022818.01.T01:CDS
AGCATAAAGCATCCAAACAC+TGG 0.464705 4:-77062866 None:intergenic
GTGTTGGTTTCGTCATCGTC+AGG 0.468497 4:+77062207 MsG0480022818.01.T01:CDS
AAATCCACAGCACTTGCATC+TGG 0.469906 4:+77062290 MsG0480022818.01.T01:CDS
CGCCACGCTGCTTCGTTACG+TGG 0.470138 4:+77063029 MsG0480022818.01.T01:CDS
TGTTCTGCCATGCGTGAAGC+AGG 0.474796 4:+77062231 MsG0480022818.01.T01:CDS
TCCCTTGGAACCTATGCCCC+TGG 0.504369 4:+77062600 MsG0480022818.01.T01:CDS
ATCAATAATAACAAAGTCGA+TGG 0.512053 4:-77062133 None:intergenic
TTCCAGGGGCATAGGTTCCA+AGG 0.514262 4:-77062602 None:intergenic
CATTAATTCATCTAGTCCGC+TGG 0.519273 4:-77063006 None:intergenic
AGTAGTATTTCCAGGGGCAT+AGG 0.521451 4:-77062610 None:intergenic
TTCTATCTTTCACAGCCTGA+AGG 0.537447 4:-77062902 None:intergenic
ATATTTGCGTGCTCTTACAG+GGG 0.539684 4:+77062965 MsG0480022818.01.T01:CDS
AGTAGAAGTGCACATATGGT+TGG 0.543624 4:+77062477 MsG0480022818.01.T01:CDS
TTCAGTAGAGGTAGAAGAGT+AGG 0.553901 4:-77062261 None:intergenic
GCTTTATGCTTAAACAGTAA+CGG 0.558496 4:+77062879 MsG0480022818.01.T01:CDS
GCATAAAGCATCCAAACACT+GGG 0.565842 4:-77062865 None:intergenic
TCTAAAACGGGGAAACAAGT+AGG 0.570833 4:-77062762 None:intergenic
AAGCTTTCATCAGCAATCTC+CGG 0.571737 4:-77062563 None:intergenic
TTCGTTACGTGGGCCTGGAG+TGG 0.573690 4:+77063040 MsG0480022818.01.T01:CDS
AGAAGTGCACATATGGTTGG+AGG 0.579984 4:+77062480 MsG0480022818.01.T01:CDS
TCAACCAGATGCAAGTGCTG+TGG 0.588295 4:-77062294 None:intergenic
AATGAATCCTGCTTCACGCA+TGG 0.593407 4:-77062238 None:intergenic
TTCAATCTTCAATCGAGAGA+TGG 0.595643 4:-77062403 None:intergenic
TCAACTAACATATCCACTCC+AGG 0.597590 4:-77063053 None:intergenic
GCCACGCTGCTTCGTTACGT+GGG 0.602980 4:+77063030 MsG0480022818.01.T01:CDS
GTCTCCCATGTTGAAAATGG+TGG 0.603177 4:+77062426 MsG0480022818.01.T01:CDS
TCCAGGGGCATAGGTTCCAA+GGG 0.604431 4:-77062601 None:intergenic
GGATTTATATCTTTCAGTAG+AGG 0.610621 4:-77062273 None:intergenic
ATATACGAGTAGTATTTCCA+GGG 0.622101 4:-77062617 None:intergenic
ATTAAAGGTTCAGTGTACAA+TGG 0.628036 4:-77062531 None:intergenic
ACACTTGCATCTTTATCCAG+CGG 0.651030 4:+77062990 MsG0480022818.01.T01:CDS
GTCCAGCTGCGTCTAAAACG+GGG 0.658265 4:-77062773 None:intergenic
GCATTGTTCAGAGATTTGGG+TGG 0.693825 4:+77062369 MsG0480022818.01.T01:CDS
TATACGAGTAGTATTTCCAG+GGG 0.715668 4:-77062616 None:intergenic
CATAAAGCATCCAAACACTG+GGG 0.731214 4:-77062864 None:intergenic
ATAAAGCATCCAAACACTGG+GGG 0.734762 4:-77062863 None:intergenic
GCCCACGTAACGAAGCAGCG+TGG 0.741403 4:-77063031 None:intergenic
AAAATTATACATAAGCACTG+AGG 0.755923 4:-77062663 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTGATACATCAAAA+TGG + Chr4:77062145-77062164 MsG0480022818.01.T01:CDS 15.0%
! ATCAATAATAACAAAGTCGA+TGG - Chr4:77062136-77062155 None:intergenic 25.0%
! ATCAATTATAGCATCCAAAA+AGG - Chr4:77062808-77062827 None:intergenic 25.0%
! TGAAAGATAGAAATTCTTTG+AGG + Chr4:77062913-77062932 MsG0480022818.01.T01:CDS 25.0%
!! AAAATTATACATAAGCACTG+AGG - Chr4:77062666-77062685 None:intergenic 25.0%
!! AACCTTTAATTTCTTATCAC+CGG + Chr4:77062544-77062563 MsG0480022818.01.T01:CDS 25.0%
!! AGTGCTTATGTATAATTTTC+AGG + Chr4:77062667-77062686 MsG0480022818.01.T01:CDS 25.0%
!! CTTTTTGTATTAAATCACTC+AGG - Chr4:77062740-77062759 None:intergenic 25.0%
ATATACGAGTAGTATTTCCA+GGG - Chr4:77062620-77062639 None:intergenic 30.0%
ATTAAAGGTTCAGTGTACAA+TGG - Chr4:77062534-77062553 None:intergenic 30.0%
GCTTTATGCTTAAACAGTAA+CGG + Chr4:77062879-77062898 MsG0480022818.01.T01:CDS 30.0%
GGAAATACTACTCGTATATA+TGG + Chr4:77062621-77062640 MsG0480022818.01.T01:CDS 30.0%
GGATTTATATCTTTCAGTAG+AGG - Chr4:77062276-77062295 None:intergenic 30.0%
TATATACGAGTAGTATTTCC+AGG - Chr4:77062621-77062640 None:intergenic 30.0%
TTTATCTCTTGTAACTGTGT+TGG + Chr4:77062191-77062210 MsG0480022818.01.T01:CDS 30.0%
CTCCGGTGATAAGAAATTAA+AGG - Chr4:77062549-77062568 None:intergenic 35.0%
GGATATGTTAGTTGAGATTC+TGG + Chr4:77063061-77063080 MsG0480022818.01.T01:CDS 35.0%
TATACGAGTAGTATTTCCAG+GGG - Chr4:77062619-77062638 None:intergenic 35.0%
TCTGGAAACCATCTCAAAAT+TGG + Chr4:77063079-77063098 MsG0480022818.01.T01:CDS 35.0%
TTCAATCTTCAATCGAGAGA+TGG - Chr4:77062406-77062425 None:intergenic 35.0%
! ATGGTCAGTTCATTTTGTTG+AGG + Chr4:77062164-77062183 MsG0480022818.01.T01:CDS 35.0%
! CTAGACTCAGAATTTTCATC+TGG - Chr4:77062459-77062478 None:intergenic 35.0%
! CTTTCCACCATTTTCAACAT+GGG - Chr4:77062433-77062452 None:intergenic 35.0%
!! GCGCATTTTAGAAGCTTTTA+TGG + Chr4:77062326-77062345 MsG0480022818.01.T01:CDS 35.0%
AAGCTTTCATCAGCAATCTC+CGG - Chr4:77062566-77062585 None:intergenic 40.0%
ACACTTGCATCTTTATCCAG+CGG + Chr4:77062990-77063009 MsG0480022818.01.T01:CDS 40.0%
ACATATTTGCGTGCTCTTAC+AGG + Chr4:77062963-77062982 MsG0480022818.01.T01:CDS 40.0%
ATAGCATCCAAAAAGGCAGA+TGG - Chr4:77062801-77062820 None:intergenic 40.0%
ATATTTGCGTGCTCTTACAG+GGG + Chr4:77062965-77062984 MsG0480022818.01.T01:CDS 40.0%
CATATTTGCGTGCTCTTACA+GGG + Chr4:77062964-77062983 MsG0480022818.01.T01:CDS 40.0%
CATTAATTCATCTAGTCCGC+TGG - Chr4:77063009-77063028 None:intergenic 40.0%
GAAGTCTCCCATGTTGAAAA+TGG + Chr4:77062423-77062442 MsG0480022818.01.T01:CDS 40.0%
GCTGCATTGTTCAGAGATTT+GGG + Chr4:77062366-77062385 MsG0480022818.01.T01:CDS 40.0%
TCAACTAACATATCCACTCC+AGG - Chr4:77063056-77063075 None:intergenic 40.0%
TGCTGCATTGTTCAGAGATT+TGG + Chr4:77062365-77062384 MsG0480022818.01.T01:CDS 40.0%
TTATTGCGTGCTATATCCCT+TGG + Chr4:77062585-77062604 MsG0480022818.01.T01:CDS 40.0%
TTCAGTAGAGGTAGAAGAGT+AGG - Chr4:77062264-77062283 None:intergenic 40.0%
TTCTATCTTTCACAGCCTGA+AGG - Chr4:77062905-77062924 None:intergenic 40.0%
! AAGAGCACGCAAATATGTTC+TGG - Chr4:77062961-77062980 None:intergenic 40.0%
! AGCATAAAGCATCCAAACAC+TGG - Chr4:77062869-77062888 None:intergenic 40.0%
! AGTAGAAGTGCACATATGGT+TGG + Chr4:77062477-77062496 MsG0480022818.01.T01:CDS 40.0%
! ATAAAGCATCCAAACACTGG+GGG - Chr4:77062866-77062885 None:intergenic 40.0%
! CATAAAGCATCCAAACACTG+GGG - Chr4:77062867-77062886 None:intergenic 40.0%
! GCATAAAGCATCCAAACACT+GGG - Chr4:77062868-77062887 None:intergenic 40.0%
! GCTTTCCACCATTTTCAACA+TGG - Chr4:77062434-77062453 None:intergenic 40.0%
! GTCTAGTAGAAGTGCACATA+TGG + Chr4:77062473-77062492 MsG0480022818.01.T01:CDS 40.0%
! TCTAAAACGGGGAAACAAGT+AGG - Chr4:77062765-77062784 None:intergenic 40.0%
! TTCAGGCGAGCAAAAGATTT+TGG + Chr4:77062684-77062703 MsG0480022818.01.T01:CDS 40.0%
!! TGGCAGAACCAATTTTGAGA+TGG - Chr4:77063090-77063109 None:intergenic 40.0%
AAATCCACAGCACTTGCATC+TGG + Chr4:77062290-77062309 MsG0480022818.01.T01:CDS 45.0%
AAGTCCAGCTGCGTCTAAAA+CGG - Chr4:77062778-77062797 None:intergenic 45.0%
AATGAATCCTGCTTCACGCA+TGG - Chr4:77062241-77062260 None:intergenic 45.0%
CTTAAACAGTAACGGCCTTC+AGG + Chr4:77062887-77062906 MsG0480022818.01.T01:CDS 45.0%
GCATTGTTCAGAGATTTGGG+TGG + Chr4:77062369-77062388 MsG0480022818.01.T01:CDS 45.0%
GTCTCCCATGTTGAAAATGG+TGG + Chr4:77062426-77062445 MsG0480022818.01.T01:CDS 45.0%
TGGTTGGAGGTTCTTCAGTT+TGG + Chr4:77062493-77062512 MsG0480022818.01.T01:CDS 45.0%
! AGAAGTGCACATATGGTTGG+AGG + Chr4:77062480-77062499 MsG0480022818.01.T01:CDS 45.0%
! AGGCGAGCAAAAGATTTTGG+TGG + Chr4:77062687-77062706 MsG0480022818.01.T01:CDS 45.0%
! AGTAGTATTTCCAGGGGCAT+AGG - Chr4:77062613-77062632 None:intergenic 45.0%
! TGGACTTCCATCTGCCTTTT+TGG + Chr4:77062791-77062810 MsG0480022818.01.T01:CDS 45.0%
!! CGAGCAAAAGATTTTGGTGG+TGG + Chr4:77062690-77062709 MsG0480022818.01.T01:CDS 45.0%
ACACTGGGGGATGCATGTAA+TGG - Chr4:77062853-77062872 None:intergenic 50.0%
TACATGCATCCCCCAGTGTT+TGG + Chr4:77062854-77062873 MsG0480022818.01.T01:CDS 50.0%
TCTCTGAACAATGCAGCAGC+TGG - Chr4:77062363-77062382 None:intergenic 50.0%
! AGTCCAGCTGCGTCTAAAAC+GGG - Chr4:77062777-77062796 None:intergenic 50.0%
! TCAACCAGATGCAAGTGCTG+TGG - Chr4:77062297-77062316 None:intergenic 50.0%
!! GTGTTGGTTTCGTCATCGTC+AGG + Chr4:77062207-77062226 MsG0480022818.01.T01:CDS 50.0%
TGTTCTGCCATGCGTGAAGC+AGG + Chr4:77062231-77062250 MsG0480022818.01.T01:CDS 55.0%
! GTCCAGCTGCGTCTAAAACG+GGG - Chr4:77062776-77062795 None:intergenic 55.0%
! TCCAGGGGCATAGGTTCCAA+GGG - Chr4:77062604-77062623 None:intergenic 55.0%
! TTCCAGGGGCATAGGTTCCA+AGG - Chr4:77062605-77062624 None:intergenic 55.0%
!! TTCCCCGTTTTAGACGCAGC+TGG + Chr4:77062771-77062790 MsG0480022818.01.T01:CDS 55.0%
GCCACGCTGCTTCGTTACGT+GGG + Chr4:77063030-77063049 MsG0480022818.01.T01:CDS 60.0%
TCCCTTGGAACCTATGCCCC+TGG + Chr4:77062600-77062619 MsG0480022818.01.T01:CDS 60.0%
TTCGTTACGTGGGCCTGGAG+TGG + Chr4:77063040-77063059 MsG0480022818.01.T01:CDS 60.0%
CGCCACGCTGCTTCGTTACG+TGG + Chr4:77063029-77063048 MsG0480022818.01.T01:CDS 65.0%
GCCCACGTAACGAAGCAGCG+TGG - Chr4:77063034-77063053 None:intergenic 65.0%
GCTGCTTCGTTACGTGGGCC+TGG + Chr4:77063035-77063054 MsG0480022818.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 77062123 77063112 77062123 ID=MsG0480022818.01;Name=MsG0480022818.01
Chr4 mRNA 77062123 77063112 77062123 ID=MsG0480022818.01.T01;Parent=MsG0480022818.01;Name=MsG0480022818.01.T01;_AED=0.48;_eAED=0.74;_QI=0|0|0|1|1|1|4|0|329
Chr4 exon 77062123 77063112 77062123 ID=MsG0480022818.01.T01:exon:19904;Parent=MsG0480022818.01.T01
Chr4 CDS 77062123 77063112 77062123 ID=MsG0480022818.01.T01:cds;Parent=MsG0480022818.01.T01
Gene Sequence

>MsG0480022818.01.T01

ATGCAAAAATCCATCGACTTTGTTATTATTGATACATCAAAATGGTCAGTTCATTTTGTTGAGGCTCTTTTATCTCTTGTAACTGTGTTGGTTTCGTCATCGTCAGGATGTTCTGCCATGCGTGAAGCAGGATTCATTCCTACTCTTCTACCTCTACTGAAAGATATAAATCCACAGCACTTGCATCTGGTTGAAAAAGCTGTGCGCATTTTAGAAGCTTTTATGGATTACAGTAATCCAGCTGCTGCATTGTTCAGAGATTTGGGTGGTTTAGATGATACCATCTCTCGATTGAAGATTGAAGTCTCCCATGTTGAAAATGGTGGAAAGCAGCCAGATGAAAATTCTGAGTCTAGTAGAAGTGCACATATGGTTGGAGGTTCTTCAGTTTGGCTAGATGACATGCAGCCATTGTACACTGAACCTTTAATTTCTTATCACCGGAGATTGCTGATGAAAGCTTTATTGCGTGCTATATCCCTTGGAACCTATGCCCCTGGAAATACTACTCGTATATATGGATCTGAAGAGAATGTGCTGCCTCAGTGCTTATGTATAATTTTCAGGCGAGCAAAAGATTTTGGTGGTGGAGTTTTCTCGCTTGCTGCTACTGTCCTGAGTGATTTAATACAAAAAGATCCTACTTGTTTCCCCGTTTTAGACGCAGCTGGACTTCCATCTGCCTTTTTGGATGCTATAATTGATGATGTTCTCAACTCTTCTGAAGCCATTACATGCATCCCCCAGTGTTTGGATGCTTTATGCTTAAACAGTAACGGCCTTCAGGCTGTGAAAGATAGAAATTCTTTGAGGTGTTTTGTGAAAGTGTTTACTTCCAGAACATATTTGCGTGCTCTTACAGGGGAAACACTTGCATCTTTATCCAGCGGACTAGATGAATTAATGCGCCACGCTGCTTCGTTACGTGGGCCTGGAGTGGATATGTTAGTTGAGATTCTGGAAACCATCTCAAAATTGGTTCTGCCATAG

Protein sequence

>MsG0480022818.01.T01

MQKSIDFVIIDTSKWSVHFVEALLSLVTVLVSSSSGCSAMREAGFIPTLLPLLKDINPQHLHLVEKAVRILEAFMDYSNPAAALFRDLGGLDDTISRLKIEVSHVENGGKQPDENSESSRSAHMVGGSSVWLDDMQPLYTEPLISYHRRLLMKALLRAISLGTYAPGNTTRIYGSEENVLPQCLCIIFRRAKDFGGGVFSLAATVLSDLIQKDPTCFPVLDAAGLPSAFLDAIIDDVLNSSEAITCIPQCLDALCLNSNGLQAVKDRNSLRCFVKVFTSRTYLRALTGETLASLSSGLDELMRHAASLRGPGVDMLVEILETISKLVLP*