AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022816.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022816.01.T01 MTR_4g094705 98.405 439 7 0 188 626 1346 1784 0.0 874
MsG0480022816.01.T01 MTR_4g094705 98.667 150 2 0 1 150 1026 1175 6.17e-91 309
MsG0480022816.01.T01 MTR_4g094708 87.699 439 54 0 188 626 1355 1793 0.0 767
MsG0480022816.01.T01 MTR_4g094708 85.333 150 21 1 1 150 1036 1184 9.62e-76 265
MsG0480022816.01.T01 MTR_5g066710 35.455 440 239 9 207 620 1385 1805 4.20e-62 224
MsG0480022816.01.T01 MTR_5g066710 37.333 150 90 2 4 150 1057 1205 2.68e-21 100
MsG0480022816.01.T01 MTR_3g099777 35.111 450 250 10 190 616 1378 1808 4.89e-60 218
MsG0480022816.01.T01 MTR_3g099777 37.162 148 87 3 4 150 1077 1219 1.01e-17 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022816.01.T01 AT1G55860 57.143 441 179 5 188 624 1330 1764 6.19e-152 486
MsG0480022816.01.T01 AT1G55860 68.421 152 47 1 1 151 1011 1162 1.06e-58 214
MsG0480022816.01.T01 AT1G70320 56.422 436 180 5 188 619 1332 1761 2.44e-147 473
MsG0480022816.01.T01 AT1G70320 67.763 152 48 1 1 151 1013 1164 8.34e-58 211

Find 128 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGTACACGGAACGTTTA+AGG 0.214213 4:+77017519 MsG0480022816.01.T01:CDS
TTGAGTGTGACTCTACTTAA+TGG 0.220407 4:+77019497 MsG0480022816.01.T01:CDS
GCTATGAAGTTCCTTGAAAA+TGG 0.233498 4:+77018977 MsG0480022816.01.T01:CDS
TCTGGACATTCTACTTATAT+TGG 0.246370 4:+77017379 MsG0480022816.01.T01:CDS
AATCGTCGTCGAGCCGATTC+AGG 0.250891 4:+77017286 MsG0480022816.01.T01:CDS
GTTACTGTTGGCCTGTCAAT+TGG 0.267778 4:+77017768 MsG0480022816.01.T01:CDS
CTGGACATTCTACTTATATT+GGG 0.288425 4:+77017380 MsG0480022816.01.T01:CDS
TTGACTTGCTTTCAGATATT+CGG 0.298968 4:-77019354 None:intergenic
TAGTGCTTTACTACTTATAT+TGG 0.299249 4:+77018631 MsG0480022816.01.T01:CDS
TCTGTAAAATGTAGATATCT+TGG 0.304829 4:+77017418 MsG0480022816.01.T01:CDS
TTAAGTGGTAATCGTCATTC+AGG 0.308630 4:+77019139 MsG0480022816.01.T01:CDS
GGCTCCATGCCTGATTCATC+TGG 0.311544 4:+77018710 MsG0480022816.01.T01:CDS
GAGGAAGAAAAGAAAATAGA+TGG 0.322485 4:+77018770 MsG0480022816.01.T01:CDS
AATCAGGCATGGAGCCAGTT+TGG 0.325335 4:-77018703 None:intergenic
GTAGTTTCAGAACTACTTCC+AGG 0.325611 4:-77018194 None:intergenic
CAAGTTTGTCAAGATATCTA+TGG 0.330598 4:-77018561 None:intergenic
CTGTTCTACAATTATGTTCT+CGG 0.331597 4:+77018933 MsG0480022816.01.T01:CDS
GACGAACTTCACCTTTGTTC+TGG 0.337622 4:-77018376 None:intergenic
ACTACTAGAGTGAGATTTAC+TGG 0.338054 4:-77019418 None:intergenic
TATCAAGTAGCCATGTGTTA+TGG 0.350285 4:-77017648 None:intergenic
TCTGTGGATAATTGTGCATT+AGG 0.360735 4:+77018452 MsG0480022816.01.T01:CDS
ACCTTTGGGTCTCTTGGAAC+TGG 0.364265 4:-77017798 None:intergenic
CAACTGTATCTGTAAATTGC+AGG 0.367797 4:-77018745 None:intergenic
ATTGACAGGCCAACAGTAAC+TGG 0.368677 4:-77017765 None:intergenic
TTCTTCACTGCTCTCGTAAA+GGG 0.369740 4:+77017250 MsG0480022816.01.T01:CDS
GAAATTGATCTTGGGAAGAT+AGG 0.377873 4:+77017610 MsG0480022816.01.T01:CDS
ATGTCTGAAAAGCCACAAAA+AGG 0.381616 4:+77019393 MsG0480022816.01.T01:CDS
CTGTTGTATCGACTATTGTT+TGG 0.387179 4:+77019056 MsG0480022816.01.T01:CDS
ATCAGGCATGGAGCCAGTTT+GGG 0.389777 4:-77018702 None:intergenic
GTTTCAGAGTAGTGATTTAG+TGG 0.391521 4:+77018316 MsG0480022816.01.T01:CDS
ACATCATCAGAAATTGATCT+TGG 0.392748 4:+77017601 MsG0480022816.01.T01:CDS
TTAATGTGTCCCTCTTCAAC+TGG 0.405885 4:-77018251 None:intergenic
CTGTAAAATGTAGATATCTT+GGG 0.408277 4:+77017419 MsG0480022816.01.T01:CDS
GAATCGGCTCGACGACGATT+TGG 0.408973 4:-77017283 None:intergenic
GTAGGATCTCTAGATATAAC+TGG 0.411979 4:-77019199 None:intergenic
ATCAAGTAGCCATGTGTTAT+GGG 0.412462 4:-77017647 None:intergenic
ATGGCTGTTTGTAGTGTTTG+AGG 0.418530 4:-77019094 None:intergenic
AGAAGATAAGATATTACTTT+GGG 0.420102 4:-77018398 None:intergenic
CCATGCTCCCCAGATGAATC+AGG 0.421747 4:-77018719 None:intergenic
GTTTGTCAGTTAGAGACATC+AGG 0.428109 4:+77019247 MsG0480022816.01.T01:CDS
TGGGAAGGTTGTGGATGATA+TGG 0.431109 4:+77017438 MsG0480022816.01.T01:CDS
CCAAGGAATTGCTCTTTCCC+TGG 0.439372 4:+77019025 MsG0480022816.01.T01:CDS
TTACTGTTGGCCTGTCAATT+GGG 0.442003 4:+77017769 MsG0480022816.01.T01:CDS
TGTGTGTACGCTGCAATCTC+AGG 0.443187 4:+77017824 MsG0480022816.01.T01:CDS
GTGGAAAGAGGAACCCAAAC+TGG 0.447972 4:+77018689 MsG0480022816.01.T01:CDS
AGCAAGTCAAATGATGGATC+TGG 0.450690 4:+77019367 MsG0480022816.01.T01:CDS
CAGAAGATAAGATATTACTT+TGG 0.454861 4:-77018399 None:intergenic
AGCTCAGCTTCAAAGTCCCT+TGG 0.455616 4:+77017316 MsG0480022816.01.T01:CDS
TGGCACGTCAATAGTCTTCT+CGG 0.456923 4:-77018231 None:intergenic
CTTGTTCAGCCAGTTACTGT+TGG 0.461376 4:+77017756 MsG0480022816.01.T01:CDS
TGATGAAAGTCACAAGTTGT+TGG 0.462344 4:+77018859 MsG0480022816.01.T01:CDS
GAGATACGACAAACTTTAAG+TGG 0.467365 4:+77019124 MsG0480022816.01.T01:CDS
AAATCTTCCCGGCCTTCCAT+TGG 0.470425 4:-77019475 None:intergenic
TTTCTTCACTGCTCTCGTAA+AGG 0.472705 4:+77017249 MsG0480022816.01.T01:CDS
AATGTATTGGGGAAACCTAC+TGG 0.474671 4:+77018824 MsG0480022816.01.T01:CDS
AGTCACACTCAAAATCTTCC+CGG 0.475849 4:-77019486 None:intergenic
GCTCCATGCCTGATTCATCT+GGG 0.478136 4:+77018711 MsG0480022816.01.T01:CDS
GTTCCTTGAAAATGGAGGTC+TGG 0.479356 4:+77018985 MsG0480022816.01.T01:CDS
ACAGCCTGCATGACCACAGC+AGG 0.480028 4:-77018914 None:intergenic
TGTCCCTGCTGTGGTCATGC+AGG 0.480777 4:+77018910 MsG0480022816.01.T01:CDS
ACACATTAAGAAACCTTTAG+TGG 0.488691 4:+77018265 MsG0480022816.01.T01:CDS
CATCATCAGAAATTGATCTT+GGG 0.490494 4:+77017602 MsG0480022816.01.T01:CDS
GCAGCTAAAACTTTGTCCAT+TGG 0.494564 4:+77018424 MsG0480022816.01.T01:CDS
CCAGGGAAAGAGCAATTCCT+TGG 0.497203 4:-77019025 None:intergenic
TCTTCCCGGCCTTCCATTGG+TGG 0.498345 4:-77019472 None:intergenic
AAGCTACTAGTCAGTTGCTA+TGG 0.499789 4:+77017560 MsG0480022816.01.T01:CDS
TCTGAAAGCAAGTCAAATGA+TGG 0.501565 4:+77019361 MsG0480022816.01.T01:CDS
TGAGCTAAGTGACCCTGAAT+CGG 0.502528 4:-77017299 None:intergenic
CCTGATTCATCTGGGGAGCA+TGG 0.506588 4:+77018719 MsG0480022816.01.T01:CDS
CCATGTGTTATGGGACAGCT+TGG 0.507212 4:-77017638 None:intergenic
GTTATCAAAGGAGAAAGAAA+AGG 0.510576 4:+77019288 MsG0480022816.01.T01:CDS
CATCTCTCACATTTGACAGC+AGG 0.511021 4:+77017461 MsG0480022816.01.T01:CDS
CTGGAAAGAGCCCAATTGAC+AGG 0.512329 4:-77017779 None:intergenic
AGGAAGAAAAGAAAATAGAT+GGG 0.517160 4:+77018771 MsG0480022816.01.T01:CDS
ATCGTCGTCGAGCCGATTCA+GGG 0.524491 4:+77017287 MsG0480022816.01.T01:CDS
GATACAACAGTGTCATAGCC+AGG 0.525275 4:-77019043 None:intergenic
AAGATTGAAAATGTGGAAAG+AGG 0.530302 4:+77018677 MsG0480022816.01.T01:CDS
GTACCCACCAATGGAAGGCC+GGG 0.533805 4:+77019468 MsG0480022816.01.T01:CDS
TTGTAGCTAAAACAGTTCCA+AGG 0.535364 4:-77017333 None:intergenic
AGAAAAGAAAATAGATGGGA+GGG 0.542058 4:+77018775 MsG0480022816.01.T01:CDS
TAAGTGGTAATCGTCATTCA+GGG 0.543015 4:+77019140 MsG0480022816.01.T01:CDS
ATTCAGGGCGTGTTTCTCCC+CGG 0.543256 4:+77019155 MsG0480022816.01.T01:CDS
AGTACCCACCAATGGAAGGC+CGG 0.545617 4:+77019467 MsG0480022816.01.T01:CDS
CAAAAGTTGTTATACTGCAA+TGG 0.546442 4:+77017486 MsG0480022816.01.T01:CDS
TCCAGTTCCAAGAGACCCAA+AGG 0.549898 4:+77017797 MsG0480022816.01.T01:CDS
TAGATATCTTGGGAAGGTTG+TGG 0.551242 4:+77017429 MsG0480022816.01.T01:CDS
GCAAGTATATCATCCACTAA+AGG 0.552674 4:-77018278 None:intergenic
TATTGACGTGCCAGTTGAAG+AGG 0.555410 4:+77018241 MsG0480022816.01.T01:CDS
GACAGTAGTTATGTTATCAA+AGG 0.557320 4:+77019276 MsG0480022816.01.T01:CDS
AAGGCCAAAGATTGAAAATG+TGG 0.558391 4:+77018670 MsG0480022816.01.T01:CDS
TGGATCAAATGCTGCAGTCA+AGG 0.559311 4:+77018651 MsG0480022816.01.T01:CDS
TAGTTTCAGAACTACTTCCA+GGG 0.569502 4:-77018193 None:intergenic
AAGAAAAGAAAATAGATGGG+AGG 0.575068 4:+77018774 MsG0480022816.01.T01:CDS
CTTCCCGGCCTTCCATTGGT+GGG 0.575864 4:-77019471 None:intergenic
ACAGGCGCTTGCTTTATCCC+TGG 0.579253 4:+77018176 MsG0480022816.01.T01:intron
CTCCATGCCTGATTCATCTG+GGG 0.579816 4:+77018712 MsG0480022816.01.T01:CDS
TTTGTCAGTTAGAGACATCA+GGG 0.579885 4:+77019248 MsG0480022816.01.T01:CDS
CTCCCCAGATGAATCAGGCA+TGG 0.580775 4:-77018714 None:intergenic
TGTAGCTAAAACAGTTCCAA+GGG 0.581025 4:-77017332 None:intergenic
ACTACAAACAGCCATGGAGT+TGG 0.583178 4:+77019102 MsG0480022816.01.T01:CDS
TCAATAGTTGCAAAGCCAGT+AGG 0.586776 4:-77018839 None:intergenic
AACTGTATCTGTAAATTGCA+GGG 0.587750 4:-77018744 None:intergenic
ATTGATCTTGGGAAGATAGG+AGG 0.594251 4:+77017613 MsG0480022816.01.T01:CDS
TCTGATGATGTGATAGAGCA+CGG 0.600120 4:-77017589 None:intergenic
GCAGCTTTCATGAAAACAGT+AGG 0.602792 4:-77019217 None:intergenic
TGAAAGACAGTCTCAAGTTG+AGG 0.605486 4:+77018582 MsG0480022816.01.T01:CDS
TTGTCGTATCTCCAACTCCA+TGG 0.606847 4:-77019113 None:intergenic
CTGAAGTACCCACCAATGGA+AGG 0.612088 4:+77019463 MsG0480022816.01.T01:CDS
AGCACTAATGCATTTAGAGA+CGG 0.614014 4:-77018615 None:intergenic
TGTCAGTTAGAGACATCAGG+GGG 0.618218 4:+77019250 MsG0480022816.01.T01:CDS
TCAAACACTACAAACAGCCA+TGG 0.621903 4:+77019096 MsG0480022816.01.T01:CDS
CCAAGCTGTCCCATAACACA+TGG 0.623518 4:+77017638 MsG0480022816.01.T01:CDS
TGTGCTGAAGTACCCACCAA+TGG 0.629844 4:+77019459 MsG0480022816.01.T01:CDS
AGGGAAATTGCATCTCAGAA+CGG 0.631392 4:+77018524 MsG0480022816.01.T01:CDS
ATTTACAGATACAGTTGCGG+AGG 0.636989 4:+77018751 MsG0480022816.01.T01:CDS
TTATCCACAGAAAAGTCCAA+TGG 0.637448 4:-77018440 None:intergenic
GCAATTTACAGATACAGTTG+CGG 0.654962 4:+77018748 MsG0480022816.01.T01:CDS
ACACTTTGCAACCAGAACAA+AGG 0.655632 4:+77018365 MsG0480022816.01.T01:CDS
ATGAAGTTCCTTGAAAATGG+AGG 0.661524 4:+77018980 MsG0480022816.01.T01:CDS
ATTGACGTGCCAGTTGAAGA+GGG 0.662650 4:+77018242 MsG0480022816.01.T01:CDS
ATACAACAGTGTCATAGCCA+GGG 0.663229 4:-77019042 None:intergenic
AAAATGTAGATATCTTGGGA+AGG 0.664461 4:+77017423 MsG0480022816.01.T01:CDS
CTCTAACTGACAAACTGCAG+CGG 0.667811 4:-77019239 None:intergenic
ATCTAAATCATCGAGTACTG+AGG 0.670041 4:+77019315 MsG0480022816.01.T01:CDS
CAGCCTGCATGACCACAGCA+GGG 0.691046 4:-77018913 None:intergenic
AAAACAACATGTCCCTGCTG+TGG 0.707104 4:+77018901 MsG0480022816.01.T01:CDS
TTGTCAGTTAGAGACATCAG+GGG 0.713699 4:+77019249 MsG0480022816.01.T01:CDS
GTCAGTTAGAGACATCAGGG+GGG 0.796291 4:+77019251 MsG0480022816.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGATAAGATATTACTTT+GGG - Chr4:77018401-77018420 None:intergenic 20.0%
!! CAAAATCGAAATAACATTAT+GGG + Chr4:77017966-77017985 MsG0480022816.01.T01:intron 20.0%
!!! ATGGATTTTGAAAATGTATT+GGG + Chr4:77018812-77018831 MsG0480022816.01.T01:CDS 20.0%
!!! GTTAATAACTTTTATGTACA+CGG + Chr4:77017508-77017527 MsG0480022816.01.T01:CDS 20.0%
! AGGAAGAAAAGAAAATAGAT+GGG + Chr4:77018771-77018790 MsG0480022816.01.T01:CDS 25.0%
! CAGAAGATAAGATATTACTT+TGG - Chr4:77018402-77018421 None:intergenic 25.0%
! CTGTAAAATGTAGATATCTT+GGG + Chr4:77017419-77017438 MsG0480022816.01.T01:CDS 25.0%
! GCAAAATCGAAATAACATTA+TGG + Chr4:77017965-77017984 MsG0480022816.01.T01:intron 25.0%
! TAGTGCTTTACTACTTATAT+TGG + Chr4:77018631-77018650 MsG0480022816.01.T01:CDS 25.0%
! TCTGTAAAATGTAGATATCT+TGG + Chr4:77017418-77017437 MsG0480022816.01.T01:CDS 25.0%
!! TATAGCATTACTTCTTTTTG+AGG + Chr4:77018490-77018509 MsG0480022816.01.T01:CDS 25.0%
!! TGAAACAAATAGTGTTGAAA+TGG + Chr4:77018095-77018114 MsG0480022816.01.T01:intron 25.0%
!! TTTAGCTACAAATTTTCTTG+AGG + Chr4:77017345-77017364 MsG0480022816.01.T01:CDS 25.0%
!!! CATGGATTTTGAAAATGTAT+TGG + Chr4:77018811-77018830 MsG0480022816.01.T01:CDS 25.0%
!!! TGGATTTTGAAAATGTATTG+GGG + Chr4:77018813-77018832 MsG0480022816.01.T01:CDS 25.0%
AAAATGTAGATATCTTGGGA+AGG + Chr4:77017423-77017442 MsG0480022816.01.T01:CDS 30.0%
AACTGTATCTGTAAATTGCA+GGG - Chr4:77018747-77018766 None:intergenic 30.0%
AAGAAAAGAAAATAGATGGG+AGG + Chr4:77018774-77018793 MsG0480022816.01.T01:CDS 30.0%
AAGATTGAAAATGTGGAAAG+AGG + Chr4:77018677-77018696 MsG0480022816.01.T01:CDS 30.0%
ACACATTAAGAAACCTTTAG+TGG + Chr4:77018265-77018284 MsG0480022816.01.T01:CDS 30.0%
ACATCATCAGAAATTGATCT+TGG + Chr4:77017601-77017620 MsG0480022816.01.T01:CDS 30.0%
AGAAAAGAAAATAGATGGGA+GGG + Chr4:77018775-77018794 MsG0480022816.01.T01:CDS 30.0%
AGAATGTCCAGAAAAACTAA+GGG - Chr4:77017371-77017390 None:intergenic 30.0%
ATAATTGATGCAATGAAACC+AGG - Chr4:77017903-77017922 None:intergenic 30.0%
ATTTCCACTATTGTTGAGAT+GGG + Chr4:77018034-77018053 MsG0480022816.01.T01:intron 30.0%
CAAAAGTTGTTATACTGCAA+TGG + Chr4:77017486-77017505 MsG0480022816.01.T01:CDS 30.0%
CAAGTTTGTCAAGATATCTA+TGG - Chr4:77018564-77018583 None:intergenic 30.0%
CATCATCAGAAATTGATCTT+GGG + Chr4:77017602-77017621 MsG0480022816.01.T01:CDS 30.0%
CTGGACATTCTACTTATATT+GGG + Chr4:77017380-77017399 MsG0480022816.01.T01:CDS 30.0%
CTGTTCTACAATTATGTTCT+CGG + Chr4:77018933-77018952 MsG0480022816.01.T01:CDS 30.0%
CTTGTTACACATGTATATTC+TGG + Chr4:77017927-77017946 MsG0480022816.01.T01:intron 30.0%
GACAGTAGTTATGTTATCAA+AGG + Chr4:77019276-77019295 MsG0480022816.01.T01:CDS 30.0%
GAGGAAGAAAAGAAAATAGA+TGG + Chr4:77018770-77018789 MsG0480022816.01.T01:CDS 30.0%
GTTAACAAAAACACATGCTT+TGG + Chr4:77018955-77018974 MsG0480022816.01.T01:CDS 30.0%
GTTATCAAAGGAGAAAGAAA+AGG + Chr4:77019288-77019307 MsG0480022816.01.T01:CDS 30.0%
TAGAATGTCCAGAAAAACTA+AGG - Chr4:77017372-77017391 None:intergenic 30.0%
TCTGGACATTCTACTTATAT+TGG + Chr4:77017379-77017398 MsG0480022816.01.T01:CDS 30.0%
! AAATAGTGTTGAAATGGCTA+TGG + Chr4:77018101-77018120 MsG0480022816.01.T01:intron 30.0%
! CTTTCCACATTTTCAATCTT+TGG - Chr4:77018677-77018696 None:intergenic 30.0%
!! TTGACTTGCTTTCAGATATT+CGG - Chr4:77019357-77019376 None:intergenic 30.0%
AAAACCCATCTCAACAATAG+TGG - Chr4:77018041-77018060 None:intergenic 35.0%
AAAGATGTCAAAAATGACCG+GGG - Chr4:77019175-77019194 None:intergenic 35.0%
AAGGCCAAAGATTGAAAATG+TGG + Chr4:77018670-77018689 MsG0480022816.01.T01:CDS 35.0%
ACACATGTATATTCTGGTGT+TGG + Chr4:77017933-77017952 MsG0480022816.01.T01:intron 35.0%
ACTACTAGAGTGAGATTTAC+TGG - Chr4:77019421-77019440 None:intergenic 35.0%
AGCACTAATGCATTTAGAGA+CGG - Chr4:77018618-77018637 None:intergenic 35.0%
AGGACTACAACTACAAAACA+TGG - Chr4:77017883-77017902 None:intergenic 35.0%
ATCAAGTAGCCATGTGTTAT+GGG - Chr4:77017650-77017669 None:intergenic 35.0%
ATGAAGTTCCTTGAAAATGG+AGG + Chr4:77018980-77018999 MsG0480022816.01.T01:CDS 35.0%
ATGTCTGAAAAGCCACAAAA+AGG + Chr4:77019393-77019412 MsG0480022816.01.T01:CDS 35.0%
CAAAGATGTCAAAAATGACC+GGG - Chr4:77019176-77019195 None:intergenic 35.0%
CAACTGTATCTGTAAATTGC+AGG - Chr4:77018748-77018767 None:intergenic 35.0%
CATTTCCACTATTGTTGAGA+TGG + Chr4:77018033-77018052 MsG0480022816.01.T01:intron 35.0%
CCAAAGATGTCAAAAATGAC+CGG - Chr4:77019177-77019196 None:intergenic 35.0%
GAAATTGATCTTGGGAAGAT+AGG + Chr4:77017610-77017629 MsG0480022816.01.T01:CDS 35.0%
GAGATACGACAAACTTTAAG+TGG + Chr4:77019124-77019143 MsG0480022816.01.T01:CDS 35.0%
GCAAGTATATCATCCACTAA+AGG - Chr4:77018281-77018300 None:intergenic 35.0%
GCAATTTACAGATACAGTTG+CGG + Chr4:77018748-77018767 MsG0480022816.01.T01:CDS 35.0%
GCTATGAAGTTCCTTGAAAA+TGG + Chr4:77018977-77018996 MsG0480022816.01.T01:CDS 35.0%
GGACTACAACTACAAAACAT+GGG - Chr4:77017882-77017901 None:intergenic 35.0%
GTAGGATCTCTAGATATAAC+TGG - Chr4:77019202-77019221 None:intergenic 35.0%
GTTTCAGAGTAGTGATTTAG+TGG + Chr4:77018316-77018335 MsG0480022816.01.T01:CDS 35.0%
TAAGTGGTAATCGTCATTCA+GGG + Chr4:77019140-77019159 MsG0480022816.01.T01:CDS 35.0%
TAGTTTCAGAACTACTTCCA+GGG - Chr4:77018196-77018215 None:intergenic 35.0%
TATCAAGTAGCCATGTGTTA+TGG - Chr4:77017651-77017670 None:intergenic 35.0%
TCTGAAAGCAAGTCAAATGA+TGG + Chr4:77019361-77019380 MsG0480022816.01.T01:CDS 35.0%
TCTGTGGATAATTGTGCATT+AGG + Chr4:77018452-77018471 MsG0480022816.01.T01:CDS 35.0%
TGATGAAAGTCACAAGTTGT+TGG + Chr4:77018859-77018878 MsG0480022816.01.T01:CDS 35.0%
TGTAGCTAAAACAGTTCCAA+GGG - Chr4:77017335-77017354 None:intergenic 35.0%
TTAAGTGGTAATCGTCATTC+AGG + Chr4:77019139-77019158 MsG0480022816.01.T01:CDS 35.0%
TTATCCACAGAAAAGTCCAA+TGG - Chr4:77018443-77018462 None:intergenic 35.0%
TTATGTACACGGAACGTTTA+AGG + Chr4:77017519-77017538 MsG0480022816.01.T01:CDS 35.0%
TTGTAGCTAAAACAGTTCCA+AGG - Chr4:77017336-77017355 None:intergenic 35.0%
TTTGTCAGTTAGAGACATCA+GGG + Chr4:77019248-77019267 MsG0480022816.01.T01:CDS 35.0%
! ACATTTTCAAAATCCATGCC+AGG - Chr4:77018809-77018828 None:intergenic 35.0%
! ATCTAAATCATCGAGTACTG+AGG + Chr4:77019315-77019334 MsG0480022816.01.T01:CDS 35.0%
! ATGTTTTGTAGTTGTAGTCC+TGG + Chr4:77017882-77017901 MsG0480022816.01.T01:intron 35.0%
! CATCTCAACAATAGTGGAAA+TGG - Chr4:77018035-77018054 None:intergenic 35.0%
! GATTTACTGGAACCTTTTTG+TGG - Chr4:77019408-77019427 None:intergenic 35.0%
!! ATTGTTGAGATGGGTTTTCA+AGG + Chr4:77018043-77018062 MsG0480022816.01.T01:intron 35.0%
!! CTGTTGTATCGACTATTGTT+TGG + Chr4:77019056-77019075 MsG0480022816.01.T01:CDS 35.0%
!! GCATTACTTCTTTTTGAGGA+TGG + Chr4:77018494-77018513 MsG0480022816.01.T01:CDS 35.0%
!! TTGTTGAGATGGGTTTTCAA+GGG + Chr4:77018044-77018063 MsG0480022816.01.T01:intron 35.0%
AAGCTACTAGTCAGTTGCTA+TGG + Chr4:77017560-77017579 MsG0480022816.01.T01:CDS 40.0%
AATGTATTGGGGAAACCTAC+TGG + Chr4:77018824-77018843 MsG0480022816.01.T01:CDS 40.0%
ACAAAAACCTTTGGGTCTCT+TGG - Chr4:77017807-77017826 None:intergenic 40.0%
ACACTTTGCAACCAGAACAA+AGG + Chr4:77018365-77018384 MsG0480022816.01.T01:CDS 40.0%
AGCGTACACACAAAAACCTT+TGG - Chr4:77017816-77017835 None:intergenic 40.0%
AGGGAAATTGCATCTCAGAA+CGG + Chr4:77018524-77018543 MsG0480022816.01.T01:CDS 40.0%
AGTCACACTCAAAATCTTCC+CGG - Chr4:77019489-77019508 None:intergenic 40.0%
ATACAACAGTGTCATAGCCA+GGG - Chr4:77019045-77019064 None:intergenic 40.0%
ATGGCTGTTTGTAGTGTTTG+AGG - Chr4:77019097-77019116 None:intergenic 40.0%
ATTGATCTTGGGAAGATAGG+AGG + Chr4:77017613-77017632 MsG0480022816.01.T01:CDS 40.0%
ATTTACAGATACAGTTGCGG+AGG + Chr4:77018751-77018770 MsG0480022816.01.T01:CDS 40.0%
GCAGCTAAAACTTTGTCCAT+TGG + Chr4:77018424-77018443 MsG0480022816.01.T01:CDS 40.0%
GCAGCTTTCATGAAAACAGT+AGG - Chr4:77019220-77019239 None:intergenic 40.0%
GCGTACACACAAAAACCTTT+GGG - Chr4:77017815-77017834 None:intergenic 40.0%
GTAGTTTCAGAACTACTTCC+AGG - Chr4:77018197-77018216 None:intergenic 40.0%
GTTTGTCAGTTAGAGACATC+AGG + Chr4:77019247-77019266 MsG0480022816.01.T01:CDS 40.0%
TAGATATCTTGGGAAGGTTG+TGG + Chr4:77017429-77017448 MsG0480022816.01.T01:CDS 40.0%
TCAAACACTACAAACAGCCA+TGG + Chr4:77019096-77019115 MsG0480022816.01.T01:CDS 40.0%
TCAATAGTTGCAAAGCCAGT+AGG - Chr4:77018842-77018861 None:intergenic 40.0%
TTAATGTGTCCCTCTTCAAC+TGG - Chr4:77018254-77018273 None:intergenic 40.0%
TTCTTCACTGCTCTCGTAAA+GGG + Chr4:77017250-77017269 MsG0480022816.01.T01:CDS 40.0%
TTGTCAGTTAGAGACATCAG+GGG + Chr4:77019249-77019268 MsG0480022816.01.T01:CDS 40.0%
TTTCTTCACTGCTCTCGTAA+AGG + Chr4:77017249-77017268 MsG0480022816.01.T01:CDS 40.0%
! TCTGATGATGTGATAGAGCA+CGG - Chr4:77017592-77017611 None:intergenic 40.0%
! TGAAAGACAGTCTCAAGTTG+AGG + Chr4:77018582-77018601 MsG0480022816.01.T01:CDS 40.0%
! TTACTGTTGGCCTGTCAATT+GGG + Chr4:77017769-77017788 MsG0480022816.01.T01:CDS 40.0%
! TTGTCCATTGGACTTTTCTG+TGG + Chr4:77018436-77018455 MsG0480022816.01.T01:CDS 40.0%
!! AGCAAGTCAAATGATGGATC+TGG + Chr4:77019367-77019386 MsG0480022816.01.T01:CDS 40.0%
!! CTGCTCTTTTTAGTCTTCCA+AGG + Chr4:77019008-77019027 MsG0480022816.01.T01:CDS 40.0%
!! TTTTGAGGATGGAAGTGCAA+GGG + Chr4:77018505-77018524 MsG0480022816.01.T01:CDS 40.0%
!! TTTTTGAGGATGGAAGTGCA+AGG + Chr4:77018504-77018523 MsG0480022816.01.T01:CDS 40.0%
!!! CCGGTCATTTTTGACATCTT+TGG + Chr4:77019174-77019193 MsG0480022816.01.T01:CDS 40.0%
AAAACAACATGTCCCTGCTG+TGG + Chr4:77018901-77018920 MsG0480022816.01.T01:CDS 45.0%
AACATGGGATGATTCCAGAC+TGG - Chr4:77017867-77017886 None:intergenic 45.0%
ACTACAAACAGCCATGGAGT+TGG + Chr4:77019102-77019121 MsG0480022816.01.T01:CDS 45.0%
AGGATGTAATCCTACCAGTC+TGG + Chr4:77017850-77017869 MsG0480022816.01.T01:intron 45.0%
ATTGACAGGCCAACAGTAAC+TGG - Chr4:77017768-77017787 None:intergenic 45.0%
ATTGACGTGCCAGTTGAAGA+GGG + Chr4:77018242-77018261 MsG0480022816.01.T01:CDS 45.0%
CATCTCTCACATTTGACAGC+AGG + Chr4:77017461-77017480 MsG0480022816.01.T01:CDS 45.0%
CTCTAACTGACAAACTGCAG+CGG - Chr4:77019242-77019261 None:intergenic 45.0%
CTTGTTCAGCCAGTTACTGT+TGG + Chr4:77017756-77017775 MsG0480022816.01.T01:CDS 45.0%
GACGAACTTCACCTTTGTTC+TGG - Chr4:77018379-77018398 None:intergenic 45.0%
GATACAACAGTGTCATAGCC+AGG - Chr4:77019046-77019065 None:intergenic 45.0%
GTGTTGAAATGGCTATGGAG+TGG + Chr4:77018106-77018125 MsG0480022816.01.T01:intron 45.0%
GTTCCTTGAAAATGGAGGTC+TGG + Chr4:77018985-77019004 MsG0480022816.01.T01:CDS 45.0%
TATTGACGTGCCAGTTGAAG+AGG + Chr4:77018241-77018260 MsG0480022816.01.T01:CDS 45.0%
TCATGCTGATGATCCTATCC+AGG + Chr4:77018137-77018156 MsG0480022816.01.T01:intron 45.0%
TGAGCTAAGTGACCCTGAAT+CGG - Chr4:77017302-77017321 None:intergenic 45.0%
TGGATCAAATGCTGCAGTCA+AGG + Chr4:77018651-77018670 MsG0480022816.01.T01:CDS 45.0%
TGGCACGTCAATAGTCTTCT+CGG - Chr4:77018234-77018253 None:intergenic 45.0%
TGGGAAGGTTGTGGATGATA+TGG + Chr4:77017438-77017457 MsG0480022816.01.T01:CDS 45.0%
TGTCAGTTAGAGACATCAGG+GGG + Chr4:77019250-77019269 MsG0480022816.01.T01:CDS 45.0%
TTGTCGTATCTCCAACTCCA+TGG - Chr4:77019116-77019135 None:intergenic 45.0%
! GTTACTGTTGGCCTGTCAAT+TGG + Chr4:77017768-77017787 MsG0480022816.01.T01:CDS 45.0%
!! AGATGGGTTTTCAAGGGCAA+GGG + Chr4:77018050-77018069 MsG0480022816.01.T01:intron 45.0%
!! CTTGAGGCCCTTAGTTTTTC+TGG + Chr4:77017361-77017380 MsG0480022816.01.T01:CDS 45.0%
AAATCTTCCCGGCCTTCCAT+TGG - Chr4:77019478-77019497 None:intergenic 50.0%
AATCAGGCATGGAGCCAGTT+TGG - Chr4:77018706-77018725 None:intergenic 50.0%
AATGGTTGCCTCATCAGGTG+AGG - Chr4:77018017-77018036 None:intergenic 50.0%
ACCTTTGGGTCTCTTGGAAC+TGG - Chr4:77017801-77017820 None:intergenic 50.0%
AGCTCAGCTTCAAAGTCCCT+TGG + Chr4:77017316-77017335 MsG0480022816.01.T01:CDS 50.0%
AGTTCGTCATCCTCCTGGAT+AGG - Chr4:77018153-77018172 None:intergenic 50.0%
ATCAGGCATGGAGCCAGTTT+GGG - Chr4:77018705-77018724 None:intergenic 50.0%
ATGGTTGCCTCATCAGGTGA+GGG - Chr4:77018016-77018035 None:intergenic 50.0%
CCAAGCTGTCCCATAACACA+TGG + Chr4:77017638-77017657 MsG0480022816.01.T01:CDS 50.0%
CCAAGGAATTGCTCTTTCCC+TGG + Chr4:77019025-77019044 MsG0480022816.01.T01:CDS 50.0%
CCAGGGAAAGAGCAATTCCT+TGG - Chr4:77019028-77019047 None:intergenic 50.0%
CCATGTGTTATGGGACAGCT+TGG - Chr4:77017641-77017660 None:intergenic 50.0%
CTCCATGCCTGATTCATCTG+GGG + Chr4:77018712-77018731 MsG0480022816.01.T01:CDS 50.0%
CTGGAAAGAGCCCAATTGAC+AGG - Chr4:77017782-77017801 None:intergenic 50.0%
GAGGGAAAAAGAACCTGGCA+TGG + Chr4:77018793-77018812 MsG0480022816.01.T01:CDS 50.0%
GATGGGAGGGAAAAAGAACC+TGG + Chr4:77018788-77018807 MsG0480022816.01.T01:CDS 50.0%
GCTCCATGCCTGATTCATCT+GGG + Chr4:77018711-77018730 MsG0480022816.01.T01:CDS 50.0%
GTCAGTTAGAGACATCAGGG+GGG + Chr4:77019251-77019270 MsG0480022816.01.T01:CDS 50.0%
GTGGAAAGAGGAACCCAAAC+TGG + Chr4:77018689-77018708 MsG0480022816.01.T01:CDS 50.0%
GTGGAAATGGTTGCCTCATC+AGG - Chr4:77018022-77018041 None:intergenic 50.0%
TACGCTGCAATCTCAGGTTC+AGG + Chr4:77017830-77017849 MsG0480022816.01.T01:intron 50.0%
TCCAGTTCCAAGAGACCCAA+AGG + Chr4:77017797-77017816 MsG0480022816.01.T01:CDS 50.0%
TGCTGATGATCCTATCCAGG+AGG + Chr4:77018140-77018159 MsG0480022816.01.T01:intron 50.0%
TGGGATGATTCCAGACTGGT+AGG - Chr4:77017863-77017882 None:intergenic 50.0%
TGTGTGTACGCTGCAATCTC+AGG + Chr4:77017824-77017843 MsG0480022816.01.T01:CDS 50.0%
! CAGCCAGACCTCCATTTTCA+AGG - Chr4:77018991-77019010 None:intergenic 50.0%
! CTGAAGTACCCACCAATGGA+AGG + Chr4:77019463-77019482 MsG0480022816.01.T01:CDS 50.0%
! TGTGCTGAAGTACCCACCAA+TGG + Chr4:77019459-77019478 MsG0480022816.01.T01:CDS 50.0%
!! GAGATGGGTTTTCAAGGGCA+AGG + Chr4:77018049-77018068 MsG0480022816.01.T01:intron 50.0%
ACAGGCGCTTGCTTTATCCC+TGG + Chr4:77018176-77018195 MsG0480022816.01.T01:intron 55.0%
AGTACCCACCAATGGAAGGC+CGG + Chr4:77019467-77019486 MsG0480022816.01.T01:CDS 55.0%
AGTTAGAGACATCAGGGGGG+AGG + Chr4:77019254-77019273 MsG0480022816.01.T01:CDS 55.0%
ATTCAGGGCGTGTTTCTCCC+CGG + Chr4:77019155-77019174 MsG0480022816.01.T01:CDS 55.0%
CCATGCTCCCCAGATGAATC+AGG - Chr4:77018722-77018741 None:intergenic 55.0%
CCTGATTCATCTGGGGAGCA+TGG + Chr4:77018719-77018738 MsG0480022816.01.T01:CDS 55.0%
CTCCCCAGATGAATCAGGCA+TGG - Chr4:77018717-77018736 None:intergenic 55.0%
GGAGGATGACGAACTTGCAC+AGG + Chr4:77018158-77018177 MsG0480022816.01.T01:intron 55.0%
GGCTCCATGCCTGATTCATC+TGG + Chr4:77018710-77018729 MsG0480022816.01.T01:CDS 55.0%
GTGCAAGTTCGTCATCCTCC+TGG - Chr4:77018158-77018177 None:intergenic 55.0%
TGGTTGCCTCATCAGGTGAG+GGG - Chr4:77018015-77018034 None:intergenic 55.0%
! AATCGTCGTCGAGCCGATTC+AGG + Chr4:77017286-77017305 MsG0480022816.01.T01:CDS 55.0%
! ATCGTCGTCGAGCCGATTCA+GGG + Chr4:77017287-77017306 MsG0480022816.01.T01:CDS 55.0%
!! CTTTGCTGTTGTCCCCACGT+CGG + Chr4:77017716-77017735 MsG0480022816.01.T01:intron 55.0%
!! GAATCGGCTCGACGACGATT+TGG - Chr4:77017286-77017305 None:intergenic 55.0%
ACAGCCTGCATGACCACAGC+AGG - Chr4:77018917-77018936 None:intergenic 60.0%
CAGCCTGCATGACCACAGCA+GGG - Chr4:77018916-77018935 None:intergenic 60.0%
CTTCCCGGCCTTCCATTGGT+GGG - Chr4:77019474-77019493 None:intergenic 60.0%
GGTTGCCTCATCAGGTGAGG+GGG - Chr4:77018014-77018033 None:intergenic 60.0%
GTACCCACCAATGGAAGGCC+GGG + Chr4:77019468-77019487 MsG0480022816.01.T01:CDS 60.0%
TCATGCCCCCTCACCTGATG+AGG + Chr4:77018006-77018025 MsG0480022816.01.T01:intron 60.0%
TCTGCCTGAGATGCCGACGT+GGG - Chr4:77017732-77017751 None:intergenic 60.0%
TCTTCCCGGCCTTCCATTGG+TGG - Chr4:77019475-77019494 None:intergenic 60.0%
TGTCCCTGCTGTGGTCATGC+AGG + Chr4:77018910-77018929 MsG0480022816.01.T01:CDS 60.0%
! GGTGAGGGGGCATGAAACGT+TGG - Chr4:77018001-77018020 None:intergenic 60.0%
CTCTGCCTGAGATGCCGACG+TGG - Chr4:77017733-77017752 None:intergenic 65.0%
CTGCCTGAGATGCCGACGTG+GGG - Chr4:77017731-77017750 None:intergenic 65.0%
TGTCCCCACGTCGGCATCTC+AGG + Chr4:77017725-77017744 MsG0480022816.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 77017241 77019516 77017241 ID=MsG0480022816.01;Name=MsG0480022816.01
Chr4 mRNA 77017241 77019516 77017241 ID=MsG0480022816.01.T01;Parent=MsG0480022816.01;Name=MsG0480022816.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|626
Chr4 exon 77017241 77017685 77017241 ID=MsG0480022816.01.T01:exon:19885;Parent=MsG0480022816.01.T01
Chr4 exon 77017747 77017845 77017747 ID=MsG0480022816.01.T01:exon:19886;Parent=MsG0480022816.01.T01
Chr4 exon 77018180 77019516 77018180 ID=MsG0480022816.01.T01:exon:19887;Parent=MsG0480022816.01.T01
Chr4 CDS 77017241 77017685 77017241 ID=MsG0480022816.01.T01:cds;Parent=MsG0480022816.01.T01
Chr4 CDS 77017747 77017845 77017747 ID=MsG0480022816.01.T01:cds;Parent=MsG0480022816.01.T01
Chr4 CDS 77018180 77019516 77018180 ID=MsG0480022816.01.T01:cds;Parent=MsG0480022816.01.T01
Gene Sequence

>MsG0480022816.01.T01

ATGCGCTCTTTCTTCACTGCTCTCGTAAAGGGATTCACTACACCAAATCGTCGTCGAGCCGATTCAGGGTCACTTAGCTCAGCTTCAAAGTCCCTTGGAACTGTTTTAGCTACAAATTTTCTTGAGGCCCTTAGTTTTTCTGGACATTCTACTTATATTGGGCTTGAAACTTCACTTTCTGTAAAATGTAGATATCTTGGGAAGGTTGTGGATGATATGGCATCTCTCACATTTGACAGCAGGCGCAAAAGTTGTTATACTGCAATGGTTAATAACTTTTATGTACACGGAACGTTTAAGGAGTTATTGACAACATTTGAAGCTACTAGTCAGTTGCTATGGACTCTTCCGTGCTCTATCACATCATCAGAAATTGATCTTGGGAAGATAGGAGGAGCCAAGCTGTCCCATAACACATGGCTACTTGATACATTACAAAGCTACTGCAGAGCTTCTTGTTCAGCCAGTTACTGTTGGCCTGTCAATTGGGCTCTTTCCAGTTCCAAGAGACCCAAAGGTTTTTGTGTGTACGCTGCAATCTCAGGCGCTTGCTTTATCCCTGGAAGTAGTTCTGAAACTACTAAAGTTGATAGTGCCGAGAAGACTATTGACGTGCCAGTTGAAGAGGGACACATTAAGAAACCTTTAGTGGATGATATACTTGCTGCATCTGCGAAGTTGTTTCAGAGTAGTGATTTAGTGGCATTTAAATTGACAGATTTGCTCGTAACACTTTGCAACCAGAACAAAGGTGAAGTTCGTCCCAAAGTAATATCTTATCTTCTGCAGCAGCTAAAACTTTGTCCATTGGACTTTTCTGTGGATAATTGTGCATTAGGTGTGTTAGCACATATTATAGCATTACTTCTTTTTGAGGATGGAAGTGCAAGGGAAATTGCATCTCAGAACGGAATTATATCTATCACCATAGATATCTTGACAAACTTGAAAGACAGTCTCAAGTTGAGGAAAGAAATATCCGTCTCTAAATGCATTAGTGCTTTACTACTTATATTGGATCAAATGCTGCAGTCAAGGCCAAAGATTGAAAATGTGGAAAGAGGAACCCAAACTGGCTCCATGCCTGATTCATCTGGGGAGCATGGTTCCCTGCAATTTACAGATACAGTTGCGGAGGAAGAAAAGAAAATAGATGGGAGGGAAAAAGAACCTGGCATGGATTTTGAAAATGTATTGGGGAAACCTACTGGCTTTGCAACTATTGATGAAAGTCACAAGTTGTTGGATATTACATGTGATTTGATAAAACAACATGTCCCTGCTGTGGTCATGCAGGCTGTTCTACAATTATGTTCTCGGTTAACAAAAACACATGCTTTGGCTATGAAGTTCCTTGAAAATGGAGGTCTGGCTGCTCTTTTTAGTCTTCCAAGGAATTGCTCTTTCCCTGGCTATGACACTGTTGTATCGACTATTGTTTGGCATCTTCTTGAAGATCCTCAAACACTACAAACAGCCATGGAGTTGGAGATACGACAAACTTTAAGTGGTAATCGTCATTCAGGGCGTGTTTCTCCCCGGTCATTTTTGACATCTTTGGCGCCAGTTATATCTAGAGATCCTACTGTTTTCATGAAAGCTGCCGCTGCAGTTTGTCAGTTAGAGACATCAGGGGGGAGGACAGTAGTTATGTTATCAAAGGAGAAAGAAAAGGAAAAATCTAAATCATCGAGTACTGAGGCTACAAATGAATGTCTCCGAATATCTGAAAGCAAGTCAAATGATGGATCTGGAAAATGTCTGAAAAGCCACAAAAAGGTTCCAGTAAATCTCACTCTAGTAGTTGATCAACTTCTTGAGATTGTGCTGAAGTACCCACCAATGGAAGGCCGGGAAGATTTTGAGTGTGACTCTACTTAA
Protein sequence

>MsG0480022816.01.T01

MRSFFTALVKGFTTPNRRRADSGSLSSASKSLGTVLATNFLEALSFSGHSTYIGLETSLSVKCRYLGKVVDDMASLTFDSRRKSCYTAMVNNFYVHGTFKELLTTFEATSQLLWTLPCSITSSEIDLGKIGGAKLSHNTWLLDTLQSYCRASCSASYCWPVNWALSSSKRPKGFCVYAAISGACFIPGSSSETTKVDSAEKTIDVPVEEGHIKKPLVDDILAASAKLFQSSDLVAFKLTDLLVTLCNQNKGEVRPKVISYLLQQLKLCPLDFSVDNCALGVLAHIIALLLFEDGSAREIASQNGIISITIDILTNLKDSLKLRKEISVSKCISALLLILDQMLQSRPKIENVERGTQTGSMPDSSGEHGSLQFTDTVAEEEKKIDGREKEPGMDFENVLGKPTGFATIDESHKLLDITCDLIKQHVPAVVMQAVLQLCSRLTKTHALAMKFLENGGLAALFSLPRNCSFPGYDTVVSTIVWHLLEDPQTLQTAMELEIRQTLSGNRHSGRVSPRSFLTSLAPVISRDPTVFMKAAAAVCQLETSGGRTVVMLSKEKEKEKSKSSSTEATNECLRISESKSNDGSGKCLKSHKKVPVNLTLVVDQLLEIVLKYPPMEGREDFECDST*