AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023326.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023326.01.T01 MTR_8g090270 77.500 240 37 3 1 225 2 239 2.99e-119 352
MsG0480023326.01.T01 MTR_8g090270 95.070 142 4 1 179 320 346 484 2.19e-89 275
MsG0480023326.01.T01 MTR_8g090275 70.370 243 52 3 1 228 1 238 7.24e-108 324
MsG0480023326.01.T01 MTR_8g090275 70.922 141 38 2 179 317 342 481 6.43e-60 200
MsG0480023326.01.T01 MTR_8g090285 76.887 212 40 2 1 206 2 210 3.00e-106 320
MsG0480023326.01.T01 MTR_8g090285 65.497 171 29 2 179 320 345 514 1.31e-67 220
MsG0480023326.01.T01 MTR_5g063390 74.332 187 48 0 1 187 1 187 1.83e-95 292
MsG0480023326.01.T01 MTR_5g063390 70.000 130 35 2 179 305 346 474 4.12e-58 195
MsG0480023326.01.T01 MTR_5g063390 70.000 130 35 2 179 305 217 345 4.08e-59 194
MsG0480023326.01.T01 MTR_5g063390 62.069 58 22 0 130 187 1 58 8.15e-17 80.5
MsG0480023326.01.T01 MTR_5g019490 44.865 185 96 1 5 183 38 222 1.12e-36 137
MsG0480023326.01.T01 MTR_5g019490 45.217 115 57 1 179 293 386 494 2.21e-25 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023326.01.T01 AT5G10190 71.505 186 53 0 1 186 1 186 9.13e-90 272
MsG0480023326.01.T01 AT5G10190 71.505 186 53 0 1 186 1 186 1.68e-88 273
MsG0480023326.01.T01 AT5G10190 67.133 143 45 2 179 320 342 483 8.73e-61 201
MsG0480023326.01.T01 AT1G78130 69.948 193 52 1 1 187 1 193 3.96e-86 267
MsG0480023326.01.T01 AT1G78130 67.910 134 41 2 179 311 345 477 3.90e-57 192
MsG0480023326.01.T01 AT5G10190 67.133 143 45 2 179 320 213 354 8.12e-62 201
MsG0480023326.01.T01 AT5G10190 52.632 57 27 0 130 186 1 57 6.46e-11 63.2
MsG0480023326.01.T01 AT4G36790 50.286 175 87 0 5 179 42 216 1.30e-46 164
MsG0480023326.01.T01 AT4G36790 38.621 145 71 5 154 293 357 488 3.20e-22 97.1
MsG0480023326.01.T01 AT2G18590 40.323 186 111 0 12 197 19 204 9.25e-34 129
MsG0480023326.01.T01 AT2G18590 35.652 115 68 3 179 293 364 472 3.21e-17 82.4

Find 74 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCTCCTCCACCTTCTTAC+AGG 0.172282 4:-83756250 MsG0480023326.01.T01:intron
GGGACTATTTCTGCAAATAT+TGG 0.217177 4:+83754127 None:intergenic
GGTAAATTTAGCAGGTATTA+TGG 0.231740 4:-83756487 MsG0480023326.01.T01:CDS
ACATCAATCTTTGGTATATC+TGG 0.252770 4:-83754706 MsG0480023326.01.T01:CDS
ACAGAATTTGAAGATCCTTT+TGG 0.270812 4:+83753986 None:intergenic
CAATTGTCGAGTCAGCAATT+AGG 0.272761 4:+83754821 None:intergenic
AATTGTCGAGTCAGCAATTA+GGG 0.326671 4:+83754822 None:intergenic
AGCAACCGTGGTGTAGCTTT+TGG 0.330743 4:-83754796 MsG0480023326.01.T01:CDS
TTTGGGTGGCTTCATTTAAC+AGG 0.340179 4:-83754778 MsG0480023326.01.T01:CDS
GCAATTAGGGATTGGATTGC+TGG 0.344245 4:+83754835 None:intergenic
AGCACCAATGTTATTGTCTC+TGG 0.371382 4:+83756510 None:intergenic
AAGTTTACTAATGTACTATT+GGG 0.380827 4:+83753718 None:intergenic
TGCATTAGTTGAATCAGAAA+TGG 0.385550 4:-83753816 MsG0480023326.01.T01:CDS
ATGAAGACACATACTGCAAT+TGG 0.389599 4:+83753893 None:intergenic
GACTGAGAGATGGGGTTGAC+TGG 0.401168 4:+83756604 None:intergenic
CTTGCTCGTTCCTTGTCCCT+CGG 0.402710 4:+83753845 None:intergenic
CTAGCCTCAACATCAATCTT+TGG 0.405866 4:-83754715 MsG0480023326.01.T01:CDS
ATAGCCGTTCGCGATTTCTC+AGG 0.408991 4:+83754106 None:intergenic
TCAAGAGCTTTCAATGGGAT+TGG 0.410764 4:-83754874 MsG0480023326.01.T01:CDS
TTTGGAGGAGACTGAGAGAT+GGG 0.414854 4:+83756595 None:intergenic
GGAAACCTCGGAAGCATCAT+TGG 0.427563 4:-83754757 MsG0480023326.01.T01:CDS
CACAATCGTGCACATGTCAT+TGG 0.452926 4:-83756315 MsG0480023326.01.T01:CDS
TCGAGTCAGCAATTAGGGAT+TGG 0.455276 4:+83754827 None:intergenic
CAATTTCAAGAGCTTTCAAT+GGG 0.458162 4:-83754879 MsG0480023326.01.T01:CDS
GCGAACGGCTATATATGCTT+TGG 0.460484 4:-83754095 MsG0480023326.01.T01:CDS
CGTGCACATGTCATTGGTCT+AGG 0.460732 4:-83756309 MsG0480023326.01.T01:CDS
TTGCTCGTTCCTTGTCCCTC+GGG 0.467880 4:+83753846 None:intergenic
GCAATTTCAAGAGCTTTCAA+TGG 0.486903 4:-83754880 MsG0480023326.01.T01:CDS
AACGCTGACCCAACTGCGTT+AGG 0.491732 4:-83756408 MsG0480023326.01.T01:CDS
ATCAGAAATGGAACAACTAA+AGG 0.493730 4:-83753804 MsG0480023326.01.T01:CDS
GGTTATAAACCAATTCCAAA+AGG 0.499646 4:-83754001 MsG0480023326.01.T01:CDS
TCTCACTCTCTACCGATCCC+TGG 0.500868 4:-83756382 MsG0480023326.01.T01:CDS
GGACAAGGAACGAGCAAGAA+TGG 0.504562 4:-83753840 MsG0480023326.01.T01:CDS
TTGGTGCTGGTAAATTTAGC+AGG 0.506244 4:-83756495 MsG0480023326.01.T01:CDS
CCAATCTCTTTGTATACTCC+TGG 0.508882 4:+83756441 None:intergenic
TGAGAGAACCTAACGCAGTT+GGG 0.510746 4:+83756400 None:intergenic
CCAGGAGTATACAAAGAGAT+TGG 0.519790 4:-83756441 MsG0480023326.01.T01:CDS
CAATCTTTGGTATATCTGGT+TGG 0.529034 4:-83754702 MsG0480023326.01.T01:CDS
TGAGCTAAAACGCCAACAAT+CGG 0.541127 4:+83754034 None:intergenic
GTGAGAGAACCTAACGCAGT+TGG 0.547909 4:+83756399 None:intergenic
GGTAACAGAATGCTTGAACC+AGG 0.550482 4:+83756364 None:intergenic
TATACCAAAGATTGATGTTG+AGG 0.552826 4:+83754711 None:intergenic
AAAAGCGAGAACCTGTAAGA+AGG 0.556109 4:+83756239 None:intergenic
TAGCCGTTCGCGATTTCTCA+GGG 0.557145 4:+83754107 None:intergenic
TGGGGTTGACTGGAATGTGA+TGG 0.566170 4:+83756614 None:intergenic
AGAGACAATAACATTGGTGC+TGG 0.567209 4:-83756508 MsG0480023326.01.T01:CDS
TTATGAAACTCATGATAATG+AGG 0.568900 4:-83753756 MsG0480023326.01.T01:CDS
AAATGCGGCATCACTTGCTA+AGG 0.575912 4:-83753939 MsG0480023326.01.T01:CDS
GTAACAGAATGCTTGAACCA+GGG 0.588995 4:+83756365 None:intergenic
GAGAACCTGTAAGAAGGTGG+AGG 0.596387 4:+83756245 None:intergenic
GGGGTTGACTGGAATGTGAT+GGG 0.597061 4:+83756615 None:intergenic
TTGGAGGAGACTGAGAGATG+GGG 0.597839 4:+83756596 None:intergenic
CACGATTGTGTCGTGTGGCA+AGG 0.598295 4:+83756328 None:intergenic
GCATTGGTGAGTATCATAGT+TGG 0.598808 4:-83754658 MsG0480023326.01.T01:intron
AGAAATGGAACAACTAAAGG+TGG 0.599904 4:-83753801 MsG0480023326.01.T01:CDS
AGAATGCTTGAACCAGGGAT+CGG 0.613048 4:+83756370 None:intergenic
GAAACCAGAGACAATAACAT+TGG 0.614772 4:-83756514 MsG0480023326.01.T01:CDS
GCTGATGAGTCATTGTTACC+AGG 0.624049 4:-83756459 MsG0480023326.01.T01:CDS
GAGAACCAATGATGCTTCCG+AGG 0.625264 4:+83754752 None:intergenic
TTCTCTATTTCACATACCCG+AGG 0.625461 4:-83753862 MsG0480023326.01.T01:CDS
TGAAACTGATAGAGAAAATG+CGG 0.626937 4:-83753954 MsG0480023326.01.T01:CDS
GTGGCAAGGTAAGCAGCAAG+AGG 0.631198 4:+83756342 None:intergenic
AGCGAGAACCTGTAAGAAGG+TGG 0.634010 4:+83756242 None:intergenic
TTTCACATACCCGAGGGACA+AGG 0.635739 4:-83753855 MsG0480023326.01.T01:CDS
TTGGATTGCTGGAATAACAA+TGG 0.646285 4:+83754846 None:intergenic
TCTCTATTTCACATACCCGA+GGG 0.649176 4:-83753861 MsG0480023326.01.T01:CDS
GCTAAAACGCCAACAATCGG+TGG 0.651078 4:+83754037 None:intergenic
TGGCAAGGTAAGCAGCAAGA+GGG 0.653848 4:+83756343 None:intergenic
CTTCATTTAACAGGAAACCT+CGG 0.655826 4:-83754769 MsG0480023326.01.T01:CDS
AGTCCCTGAGAAATCGCGAA+CGG 0.681481 4:-83754110 MsG0480023326.01.T01:CDS
ATGTGCACGATTGTGTCGTG+TGG 0.695146 4:+83756323 None:intergenic
TCGACAATTGATAGCAACCG+TGG 0.696303 4:-83754808 MsG0480023326.01.T01:CDS
GCCACCCAAAAGCTACACCA+CGG 0.722794 4:+83754791 None:intergenic
CTAAAGGTGGAACATGACAG+AGG 0.739543 4:-83753788 MsG0480023326.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATTTAATTAAAATAT+AGG - Chr4:83754805-83754824 MsG0480023326.01.T01:CDS 0.0%
!!! ATTTTAATTAAATTAATTTA+TGG + Chr4:83754803-83754822 None:intergenic 0.0%
!! TTAAAATCTTATAAAATCTT+AGG - Chr4:83754321-83754340 MsG0480023326.01.T01:intron 10.0%
!! TTTATAATTTCTAATTCAAT+TGG - Chr4:83755987-83756006 MsG0480023326.01.T01:intron 10.0%
!!! TGTTTAATCTTCTTATATTT+TGG + Chr4:83755034-83755053 None:intergenic 15.0%
!!! TTTTCTATTTTTCTTGTTTA+AGG - Chr4:83754141-83754160 MsG0480023326.01.T01:CDS 15.0%
!! AAATAAAATACGAGTTGTTA+AGG + Chr4:83754626-83754645 None:intergenic 20.0%
!! AATAAAATACGAGTTGTTAA+GGG + Chr4:83754625-83754644 None:intergenic 20.0%
!! ATAATTTCTAATTCAATTGG+TGG - Chr4:83755990-83756009 MsG0480023326.01.T01:intron 20.0%
!! ATAGGGAAAAAAAAAAAAAG+TGG - Chr4:83755305-83755324 MsG0480023326.01.T01:intron 20.0%
!! ATCTTTCATTTCAGAATAAA+AGG + Chr4:83755805-83755824 None:intergenic 20.0%
!! ATTTATTGTACTAGATGTTA+TGG + Chr4:83755165-83755184 None:intergenic 20.0%
!! GATTATAGAAATGAATATCA+TGG - Chr4:83754196-83754215 MsG0480023326.01.T01:intron 20.0%
!! GCATAAAAACAATGAATATA+TGG - Chr4:83754991-83755010 MsG0480023326.01.T01:intron 20.0%
!! GGTTAATATACAAAACATAT+AGG - Chr4:83755121-83755140 MsG0480023326.01.T01:intron 20.0%
!! TTAAAATATAGGTAATGTTC+CGG - Chr4:83754816-83754835 MsG0480023326.01.T01:CDS 20.0%
!!! AAATGTAATAAGTATTGCAT+TGG - Chr4:83754865-83754884 MsG0480023326.01.T01:CDS 20.0%
!!! ATCACTCTTTTTAATTTAGT+CGG + Chr4:83755238-83755257 None:intergenic 20.0%
!!! TAAGATTTTAAAAGACTATC+AGG + Chr4:83754312-83754331 None:intergenic 20.0%
! AAACAAGTTATTGAATCAAG+TGG - Chr4:83755049-83755068 MsG0480023326.01.T01:intron 25.0%
! GCATCTAAAAAAAACACAAT+TGG - Chr4:83754261-83754280 MsG0480023326.01.T01:intron 25.0%
! TAGAAATGATCTAACTGTTA+AGG + Chr4:83754708-83754727 None:intergenic 25.0%
!! ACATGTACTTGTTACATAAT+AGG - Chr4:83755449-83755468 MsG0480023326.01.T01:intron 25.0%
!! TGTTTTGTATATTAACCCTA+CGG + Chr4:83755118-83755137 None:intergenic 25.0%
!! TTATGAAACTCATGATAATG+AGG - Chr4:83756598-83756617 MsG0480023326.01.T01:five_prime_UTR 25.0%
!!! ATCTTTGTCTTTGTTTTCTA+TGG + Chr4:83753820-83753839 None:intergenic 25.0%
!!! ATTGTTTTTATGCACTTAAC+CGG + Chr4:83754984-83755003 None:intergenic 25.0%
!!! CTATTTTTCTTGTTTAAGGA+GGG - Chr4:83754145-83754164 MsG0480023326.01.T01:intron 25.0%
!!! CTTTTTTTTGGACTCTAAAT+AGG - Chr4:83755354-83755373 MsG0480023326.01.T01:intron 25.0%
!!! GTAGTTATTTTGTTGTCTTT+TGG + Chr4:83755759-83755778 None:intergenic 25.0%
!!! GTTTATCATTTTTCACTCTT+TGG - Chr4:83755913-83755932 MsG0480023326.01.T01:intron 25.0%
!!! TAGCTCTAATTGTATAGATA+GGG - Chr4:83755288-83755307 MsG0480023326.01.T01:intron 25.0%
!!! TAGTCATTTGCACTTTTTTT+TGG - Chr4:83755342-83755361 MsG0480023326.01.T01:intron 25.0%
!!! TCAGTTCTTTGTTATGTTTT+TGG + Chr4:83753780-83753799 None:intergenic 25.0%
!!! TCTATTTTTCTTGTTTAAGG+AGG - Chr4:83754144-83754163 MsG0480023326.01.T01:intron 25.0%
AAAGACTATCAGGATTTCAA+AGG + Chr4:83754302-83754321 None:intergenic 30.0%
AATATTGGATCATTAGTTGC+TGG + Chr4:83756215-83756234 None:intergenic 30.0%
AATGTTAGCACAAATAAGTG+CGG - Chr4:83756073-83756092 MsG0480023326.01.T01:intron 30.0%
ACATCAATCTTTGGTATATC+TGG - Chr4:83755648-83755667 MsG0480023326.01.T01:intron 30.0%
ACTCTTTGGTTAGAACTAAT+AGG - Chr4:83755927-83755946 MsG0480023326.01.T01:intron 30.0%
ATCAGAAATGGAACAACTAA+AGG - Chr4:83756550-83756569 MsG0480023326.01.T01:five_prime_UTR 30.0%
ATGATCTTTGTTAAGACGAA+TGG - Chr4:83755075-83755094 MsG0480023326.01.T01:intron 30.0%
ATGGTACAACAGCAATTATT+TGG - Chr4:83755958-83755977 MsG0480023326.01.T01:intron 30.0%
CAATTTCAAGAGCTTTCAAT+GGG - Chr4:83755475-83755494 MsG0480023326.01.T01:intron 30.0%
GACTCTCAAGATTTGTAATT+TGG + Chr4:83754365-83754384 None:intergenic 30.0%
GGTAAATTTAGCAGGTATTA+TGG - Chr4:83753867-83753886 MsG0480023326.01.T01:CDS 30.0%
GGTTATAAACCAATTCCAAA+AGG - Chr4:83756353-83756372 MsG0480023326.01.T01:CDS 30.0%
TATACCAAAGATTGATGTTG+AGG + Chr4:83755646-83755665 None:intergenic 30.0%
TGAAACTGATAGAGAAAATG+CGG - Chr4:83756400-83756419 MsG0480023326.01.T01:CDS 30.0%
TGCATTAGTTGAATCAGAAA+TGG - Chr4:83756538-83756557 MsG0480023326.01.T01:exon 30.0%
TTGTGCTAACATTATTCTTC+CGG + Chr4:83756066-83756085 None:intergenic 30.0%
TTTCAGATAATTGTAGCTCA+AGG - Chr4:83755864-83755883 MsG0480023326.01.T01:intron 30.0%
! AATTCAATTGGTGGTCTATT+TGG - Chr4:83755999-83756018 MsG0480023326.01.T01:intron 30.0%
! ACAGAATTTGAAGATCCTTT+TGG + Chr4:83756371-83756390 None:intergenic 30.0%
! CTTTTTGACAAAAAGTCTAG+TGG + Chr4:83754435-83754454 None:intergenic 30.0%
! GCTACAATTATCTGAAATGA+TGG + Chr4:83755861-83755880 None:intergenic 30.0%
! TCAGAATAAAAGGACTTGTT+TGG + Chr4:83755795-83755814 None:intergenic 30.0%
!! CTAGCTCTAATTGTATAGAT+AGG - Chr4:83755287-83755306 MsG0480023326.01.T01:intron 30.0%
!! TAACACTTTTTATGCCTTTG+AGG + Chr4:83754908-83754927 None:intergenic 30.0%
!!! ACGAATGGTGGATTTTTTTT+TGG - Chr4:83755090-83755109 MsG0480023326.01.T01:intron 30.0%
!!! GATTTTTTTTTGGATCCGTA+GGG - Chr4:83755100-83755119 MsG0480023326.01.T01:intron 30.0%
!!! GGTTTGGTTTTAGTCATTTT+GGG - Chr4:83756167-83756186 MsG0480023326.01.T01:intron 30.0%
!!! GTTCTTTGTTATGTTTTTGG+AGG + Chr4:83753777-83753796 None:intergenic 30.0%
!!! GTTTTAGCTCAACATGTTTA+TGG - Chr4:83756332-83756351 MsG0480023326.01.T01:CDS 30.0%
!!! TGGTTTGGTTTTAGTCATTT+TGG - Chr4:83756166-83756185 MsG0480023326.01.T01:intron 30.0%
!!! TTGGGGATTTTTTATCTCAA+CGG - Chr4:83756030-83756049 MsG0480023326.01.T01:intron 30.0%
!!! TTTGAAGATCCTTTTGGAAT+TGG + Chr4:83756365-83756384 None:intergenic 30.0%
!!! TTTTGGGATTAATCACTTTC+TGG - Chr4:83756183-83756202 MsG0480023326.01.T01:intron 30.0%
AAAACCAAACCATGTACGAA+GGG + Chr4:83756158-83756177 None:intergenic 35.0%
AACAACAAAATTGCTGCCAA+AGG + Chr4:83756110-83756129 None:intergenic 35.0%
AACATTATTCTTCCGGTGTT+TGG + Chr4:83756059-83756078 None:intergenic 35.0%
AATTGTCGAGTCAGCAATTA+GGG + Chr4:83755535-83755554 None:intergenic 35.0%
AGAAATGGAACAACTAAAGG+TGG - Chr4:83756553-83756572 MsG0480023326.01.T01:five_prime_UTR 35.0%
AGAGACATTTGATACGATGA+AGG + Chr4:83755390-83755409 None:intergenic 35.0%
ATCTTTGTTAAGACGAATGG+TGG - Chr4:83755078-83755097 MsG0480023326.01.T01:intron 35.0%
ATGAAGACACATACTGCAAT+TGG + Chr4:83756464-83756483 None:intergenic 35.0%
ATGTTAGCACAAATAAGTGC+GGG - Chr4:83756074-83756093 MsG0480023326.01.T01:intron 35.0%
CAATCTTTGGTATATCTGGT+TGG - Chr4:83755652-83755671 MsG0480023326.01.T01:intron 35.0%
CATGGTGTAATATACAGTGT+TGG - Chr4:83754214-83754233 MsG0480023326.01.T01:intron 35.0%
CTTCATTTAACAGGAAACCT+CGG - Chr4:83755585-83755604 MsG0480023326.01.T01:intron 35.0%
GAAACCAGAGACAATAACAT+TGG - Chr4:83753840-83753859 MsG0480023326.01.T01:CDS 35.0%
GAGTATTTGGATCATTTCGA+GGG - Chr4:83755886-83755905 MsG0480023326.01.T01:intron 35.0%
GCAATTTCAAGAGCTTTCAA+TGG - Chr4:83755474-83755493 MsG0480023326.01.T01:intron 35.0%
TAAAACCAAACCATGTACGA+AGG + Chr4:83756159-83756178 None:intergenic 35.0%
TATAGTAAGTCCAACTCAGA+TGG + Chr4:83755424-83755443 None:intergenic 35.0%
TTATGTTGACATCTCCTCAA+AGG - Chr4:83754891-83754910 MsG0480023326.01.T01:intron 35.0%
TTATTCCTTAACCATTGCCT+CGG - Chr4:83754954-83754973 MsG0480023326.01.T01:intron 35.0%
TTGGATTGCTGGAATAACAA+TGG + Chr4:83755511-83755530 None:intergenic 35.0%
! AGCTTTTCACATTGTTGCAT+TGG - Chr4:83755680-83755699 MsG0480023326.01.T01:intron 35.0%
!! ATTGTAGCTCAAGGAGTATT+TGG - Chr4:83755873-83755892 MsG0480023326.01.T01:intron 35.0%
!! GGGACTATTTCTGCAAATAT+TGG + Chr4:83756230-83756249 None:intergenic 35.0%
!! TCGATCTTTTGAGAGTATGT+TGG - Chr4:83756283-83756302 MsG0480023326.01.T01:CDS 35.0%
!! TTGTTGTCTTTTGGAAAGTG+TGG + Chr4:83755750-83755769 None:intergenic 35.0%
!!! GGATTTTTTTTTGGATCCGT+AGG - Chr4:83755099-83755118 MsG0480023326.01.T01:intron 35.0%
AAAAGCGAGAACCTGTAAGA+AGG + Chr4:83754118-83754137 None:intergenic 40.0%
AGAGACAATAACATTGGTGC+TGG - Chr4:83753846-83753865 MsG0480023326.01.T01:CDS 40.0%
AGCACCAATGTTATTGTCTC+TGG + Chr4:83753847-83753866 None:intergenic 40.0%
AGTCCAACTCAGATGGTATA+AGG + Chr4:83755417-83755436 None:intergenic 40.0%
CAAAATTGCTGCCAAAGGAA+TGG + Chr4:83756105-83756124 None:intergenic 40.0%
CAACCTTATACCATCTGAGT+TGG - Chr4:83755411-83755430 MsG0480023326.01.T01:intron 40.0%
CAACTCAGATGGTATAAGGT+TGG + Chr4:83755413-83755432 None:intergenic 40.0%
CAATTGTCGAGTCAGCAATT+AGG + Chr4:83755536-83755555 None:intergenic 40.0%
CCAATCTCTTTGTATACTCC+TGG + Chr4:83753916-83753935 None:intergenic 40.0%
CTAGCCTCAACATCAATCTT+TGG - Chr4:83755639-83755658 MsG0480023326.01.T01:intron 40.0%
GAACTAATAGGCTTCTCACA+TGG - Chr4:83755939-83755958 MsG0480023326.01.T01:intron 40.0%
GTAACAGAATGCTTGAACCA+GGG + Chr4:83753992-83754011 None:intergenic 40.0%
GTCTATTTGGAGGATGGTTT+GGG - Chr4:83756012-83756031 MsG0480023326.01.T01:intron 40.0%
TATTCCTTAACCATTGCCTC+GGG - Chr4:83754955-83754974 MsG0480023326.01.T01:intron 40.0%
TCAAGAGCTTTCAATGGGAT+TGG - Chr4:83755480-83755499 MsG0480023326.01.T01:intron 40.0%
TCTATTTGGAGGATGGTTTG+GGG - Chr4:83756013-83756032 MsG0480023326.01.T01:intron 40.0%
TCTCTATTTCACATACCCGA+GGG - Chr4:83756493-83756512 MsG0480023326.01.T01:CDS 40.0%
TGAGCTAAAACGCCAACAAT+CGG + Chr4:83756323-83756342 None:intergenic 40.0%
TTCTCTATTTCACATACCCG+AGG - Chr4:83756492-83756511 MsG0480023326.01.T01:CDS 40.0%
TTTGGGTGGCTTCATTTAAC+AGG - Chr4:83755576-83755595 MsG0480023326.01.T01:intron 40.0%
! CCAGGAGTATACAAAGAGAT+TGG - Chr4:83753913-83753932 MsG0480023326.01.T01:CDS 40.0%
! GCATTGGTGAGTATCATAGT+TGG - Chr4:83755696-83755715 MsG0480023326.01.T01:intron 40.0%
! GGAGTATTTGGATCATTTCG+AGG - Chr4:83755885-83755904 MsG0480023326.01.T01:intron 40.0%
! GTTTAAGGAGGGTGTATCAA+GGG - Chr4:83754156-83754175 MsG0480023326.01.T01:intron 40.0%
! TCAATTGGTGGTCTATTTGG+AGG - Chr4:83756002-83756021 MsG0480023326.01.T01:intron 40.0%
! TGGTCTAGGTGCTTTTCTTT+GGG - Chr4:83754059-83754078 MsG0480023326.01.T01:CDS 40.0%
! TGTTTAAGGAGGGTGTATCA+AGG - Chr4:83754155-83754174 MsG0480023326.01.T01:intron 40.0%
! TTGGTCTAGGTGCTTTTCTT+TGG - Chr4:83754058-83754077 MsG0480023326.01.T01:CDS 40.0%
!! TTGGTGCTGGTAAATTTAGC+AGG - Chr4:83753859-83753878 MsG0480023326.01.T01:CDS 40.0%
AGAATGCTTGAACCAGGGAT+CGG + Chr4:83753987-83754006 None:intergenic 45.0%
ATTCCTTAACCATTGCCTCG+GGG - Chr4:83754956-83754975 MsG0480023326.01.T01:intron 45.0%
CACAATCGTGCACATGTCAT+TGG - Chr4:83754039-83754058 MsG0480023326.01.T01:CDS 45.0%
CTAAAGGTGGAACATGACAG+AGG - Chr4:83756566-83756585 MsG0480023326.01.T01:five_prime_UTR 45.0%
GCAATTAGGGATTGGATTGC+TGG + Chr4:83755522-83755541 None:intergenic 45.0%
GCGAACGGCTATATATGCTT+TGG - Chr4:83756259-83756278 MsG0480023326.01.T01:CDS 45.0%
GCTGATGAGTCATTGTTACC+AGG - Chr4:83753895-83753914 MsG0480023326.01.T01:CDS 45.0%
GGTAACAGAATGCTTGAACC+AGG + Chr4:83753993-83754012 None:intergenic 45.0%
GGTCTATTTGGAGGATGGTT+TGG - Chr4:83756011-83756030 MsG0480023326.01.T01:intron 45.0%
TCGAGTCAGCAATTAGGGAT+TGG + Chr4:83755530-83755549 None:intergenic 45.0%
TCTCAACGGTTACCAAACAC+CGG - Chr4:83756044-83756063 MsG0480023326.01.T01:intron 45.0%
TGAGAGAACCTAACGCAGTT+GGG + Chr4:83753957-83753976 None:intergenic 45.0%
TTGCTTTCTTAACCAGTGCC+CGG + Chr4:83754847-83754866 None:intergenic 45.0%
TTTGGAGGAGACTGAGAGAT+GGG + Chr4:83753762-83753781 None:intergenic 45.0%
! AAATGCGGCATCACTTGCTA+AGG - Chr4:83756415-83756434 MsG0480023326.01.T01:CDS 45.0%
! TCGACAATTGATAGCAACCG+TGG - Chr4:83755546-83755565 MsG0480023326.01.T01:intron 45.0%
! TTGGTGGTCTATTTGGAGGA+TGG - Chr4:83756006-83756025 MsG0480023326.01.T01:intron 45.0%
!! TCTTTTGCTCCACCGATTGT+TGG - Chr4:83756308-83756327 MsG0480023326.01.T01:CDS 45.0%
!! TTTTGGAGGAGACTGAGAGA+TGG + Chr4:83753763-83753782 None:intergenic 45.0%
!!! AAATTGATTAATTTTTTTTT+TGG + Chr4:83754655-83754674 None:intergenic 5.0%
!!! AATTGATTAATTTTTTTTTT+GGG + Chr4:83754654-83754673 None:intergenic 5.0%
AGCGAGAACCTGTAAGAAGG+TGG + Chr4:83754115-83754134 None:intergenic 50.0%
AGTCCCTGAGAAATCGCGAA+CGG - Chr4:83756244-83756263 MsG0480023326.01.T01:intron 50.0%
ATAGCCGTTCGCGATTTCTC+AGG + Chr4:83756251-83756270 None:intergenic 50.0%
ATGTGCACGATTGTGTCGTG+TGG + Chr4:83754034-83754053 None:intergenic 50.0%
GAGAACCAATGATGCTTCCG+AGG + Chr4:83755605-83755624 None:intergenic 50.0%
GAGAACCTGTAAGAAGGTGG+AGG + Chr4:83754112-83754131 None:intergenic 50.0%
GCTAAAACGCCAACAATCGG+TGG + Chr4:83756320-83756339 None:intergenic 50.0%
GGACAAGGAACGAGCAAGAA+TGG - Chr4:83756514-83756533 MsG0480023326.01.T01:CDS 50.0%
GGGGTTGACTGGAATGTGAT+GGG + Chr4:83753742-83753761 None:intergenic 50.0%
GTGAGAGAACCTAACGCAGT+TGG + Chr4:83753958-83753977 None:intergenic 50.0%
TAGCCGTTCGCGATTTCTCA+GGG + Chr4:83756250-83756269 None:intergenic 50.0%
TGCGGGTTCAACCATTCCTT+TGG - Chr4:83756091-83756110 MsG0480023326.01.T01:intron 50.0%
TGGCAAGGTAAGCAGCAAGA+GGG + Chr4:83754014-83754033 None:intergenic 50.0%
TGGGGTTGACTGGAATGTGA+TGG + Chr4:83753743-83753762 None:intergenic 50.0%
TGTCTCCTCCACCTTCTTAC+AGG - Chr4:83754104-83754123 MsG0480023326.01.T01:CDS 50.0%
TTGGAGGAGACTGAGAGATG+GGG + Chr4:83753761-83753780 None:intergenic 50.0%
TTTCACATACCCGAGGGACA+AGG - Chr4:83756499-83756518 MsG0480023326.01.T01:CDS 50.0%
! AAGGGCGTGGATGGATCATT+AGG + Chr4:83756140-83756159 None:intergenic 50.0%
! AGCAACCGTGGTGTAGCTTT+TGG - Chr4:83755558-83755577 MsG0480023326.01.T01:intron 50.0%
! CGTGCACATGTCATTGGTCT+AGG - Chr4:83754045-83754064 MsG0480023326.01.T01:CDS 50.0%
! GCAACCGTGGTGTAGCTTTT+GGG - Chr4:83755559-83755578 MsG0480023326.01.T01:intron 50.0%
!! GGAAACCTCGGAAGCATCAT+TGG - Chr4:83755597-83755616 MsG0480023326.01.T01:intron 50.0%
AACGCTGACCCAACTGCGTT+AGG - Chr4:83753946-83753965 MsG0480023326.01.T01:CDS 55.0%
AGGTAATGTTCCGGTGCCTC+CGG - Chr4:83754825-83754844 MsG0480023326.01.T01:CDS 55.0%
CAAACCATGTACGAAGGGCG+TGG + Chr4:83756153-83756172 None:intergenic 55.0%
CACGATTGTGTCGTGTGGCA+AGG + Chr4:83754029-83754048 None:intergenic 55.0%
CACGCCCTTCGTACATGGTT+TGG - Chr4:83756151-83756170 MsG0480023326.01.T01:intron 55.0%
CTTGCTCGTTCCTTGTCCCT+CGG + Chr4:83756512-83756531 None:intergenic 55.0%
CTTTCTTAACCAGTGCCCGG+AGG + Chr4:83754844-83754863 None:intergenic 55.0%
GACTGAGAGATGGGGTTGAC+TGG + Chr4:83753753-83753772 None:intergenic 55.0%
GCCACCCAAAAGCTACACCA+CGG + Chr4:83755566-83755585 None:intergenic 55.0%
GTGGCAAGGTAAGCAGCAAG+AGG + Chr4:83754015-83754034 None:intergenic 55.0%
TCTCACTCTCTACCGATCCC+TGG - Chr4:83753972-83753991 MsG0480023326.01.T01:CDS 55.0%
TTGCTCGTTCCTTGTCCCTC+GGG + Chr4:83756511-83756530 None:intergenic 55.0%
! ACCGTGGTGTAGCTTTTGGG+TGG - Chr4:83755562-83755581 MsG0480023326.01.T01:intron 55.0%
CCATCCACGCCCTTCGTACA+TGG - Chr4:83756146-83756165 MsG0480023326.01.T01:intron 60.0%
GGTAATGTTCCGGTGCCTCC+GGG - Chr4:83754826-83754845 MsG0480023326.01.T01:CDS 60.0%
GTGCCCCGAGGCAATGGTTA+AGG + Chr4:83754962-83754981 None:intergenic 60.0%
TAACCATTGCCTCGGGGCAC+CGG - Chr4:83754962-83754981 MsG0480023326.01.T01:intron 60.0%
! CCATGTACGAAGGGCGTGGA+TGG + Chr4:83756149-83756168 None:intergenic 60.0%
TAACCGGTGCCCCGAGGCAA+TGG + Chr4:83754968-83754987 None:intergenic 65.0%
TGCACTTAACCGGTGCCCCG+AGG + Chr4:83754974-83754993 None:intergenic 65.0%
! TAACCAGTGCCCGGAGGCAC+CGG + Chr4:83754838-83754857 None:intergenic 65.0%
GTTCCGGTGCCTCCGGGCAC+TGG - Chr4:83754832-83754851 MsG0480023326.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 83753733 83756643 83753733 ID=MsG0480023326.01;Name=MsG0480023326.01
Chr4 mRNA 83753733 83756643 83753733 ID=MsG0480023326.01.T01;Parent=MsG0480023326.01;Name=MsG0480023326.01.T01;_AED=0.49;_eAED=0.49;_QI=105|0.5|0.33|1|1|1|3|0|320
Chr4 exon 83753733 83754160 83753733 ID=MsG0480023326.01.T01:exon:5586;Parent=MsG0480023326.01.T01
Chr4 exon 83754659 83754905 83754659 ID=MsG0480023326.01.T01:exon:5585;Parent=MsG0480023326.01.T01
Chr4 exon 83756251 83756643 83756251 ID=MsG0480023326.01.T01:exon:5584;Parent=MsG0480023326.01.T01
Chr4 five_prime_UTR 83756539 83756643 83756539 ID=MsG0480023326.01.T01:five_prime_utr;Parent=MsG0480023326.01.T01
Chr4 CDS 83756251 83756538 83756251 ID=MsG0480023326.01.T01:cds;Parent=MsG0480023326.01.T01
Chr4 CDS 83754659 83754905 83754659 ID=MsG0480023326.01.T01:cds;Parent=MsG0480023326.01.T01
Chr4 CDS 83753733 83754160 83753733 ID=MsG0480023326.01.T01:cds;Parent=MsG0480023326.01.T01
Gene Sequence

>MsG0480023326.01.T01

ATGAAACCAGAGACAATAACATTGGTGCTGGTAAATTTAGCAGGTATTATGGAGAGAGCTGATGAGTCATTGTTACCAGGAGTATACAAAGAGATTGGTGATGAACTCAACGCTGACCCAACTGCGTTAGGTTCTCTCACTCTCTACCGATCCCTGGTTCAAGCATTCTGTTACCCTCTTGCTGCTTACCTTGCCACACGACACAATCGTGCACATGTCATTGGTCTAGGTGCTTTTCTTTGGGCTGCTGCTACGTTCTTCGTTGCTGTCTCCTCCACCTTCTTACAGGTTGCAATTTCAAGAGCTTTCAATGGGATTGGACTAGCCATTGTTATTCCAGCAATCCAATCCCTAATTGCTGACTCGACAATTGATAGCAACCGTGGTGTAGCTTTTGGGTGGCTTCATTTAACAGGAAACCTCGGAAGCATCATTGGTTCTCTCATTGCAGTACTACTAGCCTCAACATCAATCTTTGGTATATCTGGTTGGAGAATAGCTTTTCACATTGTTGCATTGGTGAGTATCATAGTTGCAACTAATGATCCAATATTTGCAGAAATAGTCCCTGAGAAATCGCGAACGGCTATATATGCTTTGGATCGATCTTTTGAGAGTATGTTGGCTTCTTTTGCTCCACCGATTGTTGGCGTTTTAGCTCAACATGTTTATGGTTATAAACCAATTCCAAAAGGATCTTCAAATTCTGTGAAGATCATTGAAACTGATAGAGAAAATGCGGCATCACTTGCTAAGGCACTTTATAGTACAATTGCAATTCCAATTGCAGTATGTGTCTTCATTTATTCATTTCTCTATTTCACATACCCGAGGGACAAGGAACGAGCAAGAATGGTTGCATTAGTTGAATCAGAAATGGAACAACTAAAGGTGGAACATGACAGAGGAGAAGAATATTATGAAACTCATGATAATGAGGAGAAAGTTTTGTTGTCCCAATAG
Protein sequence

>MsG0480023326.01.T01

MKPETITLVLVNLAGIMERADESLLPGVYKEIGDELNADPTALGSLTLYRSLVQAFCYPLAAYLATRHNRAHVIGLGAFLWAAATFFVAVSSTFLQVAISRAFNGIGLAIVIPAIQSLIADSTIDSNRGVAFGWLHLTGNLGSIIGSLIAVLLASTSIFGISGWRIAFHIVALVSIIVATNDPIFAEIVPEKSRTAIYALDRSFESMLASFAPPIVGVLAQHVYGYKPIPKGSSNSVKIIETDRENAASLAKALYSTIAIPIAVCVFIYSFLYFTYPRDKERARMVALVESEMEQLKVEHDRGEEYYETHDNEEKVLLSQ*