AlfalfaGEDB | MsG0480023326.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023326.01

KEGG: -
GO: GO:0016021 GO:0055085
PFAM: PF07690

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023326.01.T01	PNX99517.1	76.62	355	47	7	1	320	1	354	5.19E-179	509

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023326.01.T01	A0A2K3NWF7	76.620	355	47	7	1	320	1	354	2.48e-179	509

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049070.01	MsG0480023326.01	0.830586	2.701244e-55	2.858206e-52
MsG0180000040.01	MsG0480023326.01	0.855858	4.755940e-62	1.113467e-58
MsG0180000489.01	MsG0480023326.01	0.803194	3.916227e-49	1.955736e-46
MsG0180000490.01	MsG0480023326.01	0.805364	1.383234e-49	7.304231e-47
MsG0180000491.01	MsG0480023326.01	0.816300	5.936385e-52	4.190833e-49
MsG0180000655.01	MsG0480023326.01	0.826838	2.177622e-54	2.065675e-51
MsG0180000942.01	MsG0480023326.01	0.837070	6.457342e-57	8.293509e-54
MsG0180000976.01	MsG0480023326.01	0.858282	9.166480e-63	2.327352e-59
MsG0180001548.01	MsG0480023326.01	0.834015	3.825864e-56	4.482858e-53
MsG0180004687.01	MsG0480023326.01	0.847052	1.475622e-59	2.590613e-56

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023326.01.T01	MTR_8g090270	77.500	240	37	3	1	225	2	239	2.99e-119	352
MsG0480023326.01.T01	MTR_8g090270	95.070	142	4	1	179	320	346	484	2.19e-89	275
MsG0480023326.01.T01	MTR_8g090275	70.370	243	52	3	1	228	1	238	7.24e-108	324
MsG0480023326.01.T01	MTR_8g090275	70.922	141	38	2	179	317	342	481	6.43e-60	200
MsG0480023326.01.T01	MTR_8g090285	76.887	212	40	2	1	206	2	210	3.00e-106	320
MsG0480023326.01.T01	MTR_8g090285	65.497	171	29	2	179	320	345	514	1.31e-67	220
MsG0480023326.01.T01	MTR_5g063390	74.332	187	48	0	1	187	1	187	1.83e-95	292
MsG0480023326.01.T01	MTR_5g063390	70.000	130	35	2	179	305	346	474	4.12e-58	195
MsG0480023326.01.T01	MTR_5g063390	70.000	130	35	2	179	305	217	345	4.08e-59	194
MsG0480023326.01.T01	MTR_5g063390	62.069	58	22	0	130	187	1	58	8.15e-17	80.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023326.01.T01	AT5G10190	71.505	186	53	0	1	186	1	186	9.13e-90	272
MsG0480023326.01.T01	AT5G10190	71.505	186	53	0	1	186	1	186	1.68e-88	273
MsG0480023326.01.T01	AT5G10190	67.133	143	45	2	179	320	342	483	8.73e-61	201
MsG0480023326.01.T01	AT1G78130	69.948	193	52	1	1	187	1	193	3.96e-86	267
MsG0480023326.01.T01	AT1G78130	67.910	134	41	2	179	311	345	477	3.90e-57	192
MsG0480023326.01.T01	AT5G10190	67.133	143	45	2	179	320	213	354	8.12e-62	201
MsG0480023326.01.T01	AT5G10190	52.632	57	27	0	130	186	1	57	6.46e-11	63.2
MsG0480023326.01.T01	AT4G36790	50.286	175	87	0	5	179	42	216	1.30e-46	164
MsG0480023326.01.T01	AT4G36790	38.621	145	71	5	154	293	357	488	3.20e-22	97.1
MsG0480023326.01.T01	AT2G18590	40.323	186	111	0	12	197	19	204	9.25e-34	129

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Find 74 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCGAGAACCTGTAAGA+AGG	0.556109	4:+83756239	None:intergenic
AAATGCGGCATCACTTGCTA+AGG	0.575912	4:-83753939	MsG0480023326.01.T01:CDS
AACGCTGACCCAACTGCGTT+AGG	0.491732	4:-83756408	MsG0480023326.01.T01:CDS
AAGTTTACTAATGTACTATT+GGG	0.380827	4:+83753718	None:intergenic
AATTGTCGAGTCAGCAATTA+GGG	0.326671	4:+83754822	None:intergenic
ACAGAATTTGAAGATCCTTT+TGG	0.270812	4:+83753986	None:intergenic
ACATCAATCTTTGGTATATC+TGG	0.252770	4:-83754706	MsG0480023326.01.T01:CDS
AGAAATGGAACAACTAAAGG+TGG	0.599904	4:-83753801	MsG0480023326.01.T01:CDS
AGAATGCTTGAACCAGGGAT+CGG	0.613048	4:+83756370	None:intergenic
AGAGACAATAACATTGGTGC+TGG	0.567209	4:-83756508	MsG0480023326.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGACTATCAGGATTTCAA+AGG	+	Chr4:83754302-83754321	None:intergenic	30.0%
AATATTGGATCATTAGTTGC+TGG	+	Chr4:83756215-83756234	None:intergenic	30.0%
AATGTTAGCACAAATAAGTG+CGG	-	Chr4:83756073-83756092	MsG0480023326.01.T01:intron	30.0%
ACATCAATCTTTGGTATATC+TGG	-	Chr4:83755648-83755667	MsG0480023326.01.T01:intron	30.0%
ACTCTTTGGTTAGAACTAAT+AGG	-	Chr4:83755927-83755946	MsG0480023326.01.T01:intron	30.0%
ATCAGAAATGGAACAACTAA+AGG	-	Chr4:83756550-83756569	MsG0480023326.01.T01:five_prime_UTR	30.0%
ATGATCTTTGTTAAGACGAA+TGG	-	Chr4:83755075-83755094	MsG0480023326.01.T01:intron	30.0%
ATGGTACAACAGCAATTATT+TGG	-	Chr4:83755958-83755977	MsG0480023326.01.T01:intron	30.0%
CAATTTCAAGAGCTTTCAAT+GGG	-	Chr4:83755475-83755494	MsG0480023326.01.T01:intron	30.0%
GACTCTCAAGATTTGTAATT+TGG	+	Chr4:83754365-83754384	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	83753733	83756643	83753733	ID=MsG0480023326.01;Name=MsG0480023326.01
Chr4	mRNA	83753733	83756643	83753733	ID=MsG0480023326.01.T01;Parent=MsG0480023326.01;Name=MsG0480023326.01.T01;_AED=0.49;_eAED=0.49;_QI=105\|0.5\|0.33\|1\|1\|1\|3\|0\|320
Chr4	exon	83753733	83754160	83753733	ID=MsG0480023326.01.T01:exon:5586;Parent=MsG0480023326.01.T01
Chr4	exon	83754659	83754905	83754659	ID=MsG0480023326.01.T01:exon:5585;Parent=MsG0480023326.01.T01
Chr4	exon	83756251	83756643	83756251	ID=MsG0480023326.01.T01:exon:5584;Parent=MsG0480023326.01.T01
Chr4	five_prime_UTR	83756539	83756643	83756539	ID=MsG0480023326.01.T01:five_prime_utr;Parent=MsG0480023326.01.T01
Chr4	CDS	83756251	83756538	83756251	ID=MsG0480023326.01.T01:cds;Parent=MsG0480023326.01.T01
Chr4	CDS	83754659	83754905	83754659	ID=MsG0480023326.01.T01:cds;Parent=MsG0480023326.01.T01
Chr4	CDS	83753733	83754160	83753733	ID=MsG0480023326.01.T01:cds;Parent=MsG0480023326.01.T01

Gene Sequence

>MsG0480023326.01.T01

ATGAAACCAGAGACAATAACATTGGTGCTGGTAAATTTAGCAGGTATTATGGAGAGAGCTGATGAGTCATTGTTACCAGGAGTATACAAAGAGATTGGTGATGAACTCAACGCTGACCCAACTGCGTTAGGTTCTCTCACTCTCTACCGATCCCTGGTTCAAGCATTCTGTTACCCTCTTGCTGCTTACCTTGCCACACGACACAATCGTGCACATGTCATTGGTCTAGGTGCTTTTCTTTGGGCTGCTGCTACGTTCTTCGTTGCTGTCTCCTCCACCTTCTTACAGGTTGCAATTTCAAGAGCTTTCAATGGGATTGGACTAGCCATTGTTATTCCAGCAATCCAATCCCTAATTGCTGACTCGACAATTGATAGCAACCGTGGTGTAGCTTTTGGGTGGCTTCATTTAACAGGAAACCTCGGAAGCATCATTGGTTCTCTCATTGCAGTACTACTAGCCTCAACATCAATCTTTGGTATATCTGGTTGGAGAATAGCTTTTCACATTGTTGCATTGGTGAGTATCATAGTTGCAACTAATGATCCAATATTTGCAGAAATAGTCCCTGAGAAATCGCGAACGGCTATATATGCTTTGGATCGATCTTTTGAGAGTATGTTGGCTTCTTTTGCTCCACCGATTGTTGGCGTTTTAGCTCAACATGTTTATGGTTATAAACCAATTCCAAAAGGATCTTCAAATTCTGTGAAGATCATTGAAACTGATAGAGAAAATGCGGCATCACTTGCTAAGGCACTTTATAGTACAATTGCAATTCCAATTGCAGTATGTGTCTTCATTTATTCATTTCTCTATTTCACATACCCGAGGGACAAGGAACGAGCAAGAATGGTTGCATTAGTTGAATCAGAAATGGAACAACTAAAGGTGGAACATGACAGAGGAGAAGAATATTATGAAACTCATGATAATGAGGAGAAAGTTTTGTTGTCCCAATAG

Protein sequence

>MsG0480023326.01.T01

MKPETITLVLVNLAGIMERADESLLPGVYKEIGDELNADPTALGSLTLYRSLVQAFCYPLAAYLATRHNRAHVIGLGAFLWAAATFFVAVSSTFLQVAISRAFNGIGLAIVIPAIQSLIADSTIDSNRGVAFGWLHLTGNLGSIIGSLIAVLLASTSIFGISGWRIAFHIVALVSIIVATNDPIFAEIVPEKSRTAIYALDRSFESMLASFAPPIVGVLAQHVYGYKPIPKGSSNSVKIIETDRENAASLAKALYSTIAIPIAVCVFIYSFLYFTYPRDKERARMVALVESEMEQLKVEHDRGEEYYETHDNEEKVLLSQ*