AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049070.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049070.01.T01 MTR_8g107430 96.471 85 3 0 2 86 90 174 4.22e-56 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049070.01.T01 AT3G17930 80.723 83 16 0 4 86 84 166 4.06e-46 146

Find 18 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCAACTTGTGCTGGTTT+TGG 0.236008 contig647end:-11368 MsG0080049070.01.T01:CDS
AGGAAAGATGTCATATTAAT+CGG 0.384907 contig647end:-11595 MsG0080049070.01.T01:CDS
CTTGGCATTGCCTTAAAATC+CGG 0.397706 contig647end:-11556 MsG0080049070.01.T01:CDS
TTACAGACTAAGGCACCATT+TGG 0.404439 contig647end:-11625 None:intergenic
GGACTTGGAGTTACTTTCCT+TGG 0.438033 contig647end:-11574 MsG0080049070.01.T01:CDS
AAATGTTGTTCAACTTGTGC+TGG 0.438263 contig647end:-11374 MsG0080049070.01.T01:CDS
AGGCACCATTTGGATATTCA+AGG 0.473273 contig647end:-11615 MsG0080049070.01.T01:CDS
AGCTTGTAGTGGATCAAATC+CGG 0.498185 contig647end:+11399 None:intergenic
TCTTTCCTTGAATATCCAAA+TGG 0.501246 contig647end:+11610 None:intergenic
GATGTCATATTAATCGGACT+TGG 0.523993 contig647end:-11589 MsG0080049070.01.T01:CDS
GAGTTGAGATACCTCAAGTC+CGG 0.544969 contig647end:+11537 None:intergenic
TGCCTTAAAATCCGGACTTG+AGG 0.557648 contig647end:-11548 MsG0080049070.01.T01:intron
ACAACATTTCCAGCTTGTAG+TGG 0.569187 contig647end:+11388 None:intergenic
GGATTTGATCCACTACAAGC+TGG 0.576522 contig647end:-11397 MsG0080049070.01.T01:CDS
TGACTACGAAAGTAAAGTCA+TGG 0.582776 contig647end:-11269 MsG0080049070.01.T01:CDS
TATCTTCAGAGTCTCAAACA+AGG 0.652304 contig647end:-11317 MsG0080049070.01.T01:CDS
TTGAGATCCATCCAACAGTG+AGG 0.728057 contig647end:+11344 None:intergenic
CTACGAAAGTAAAGTCATGG+AGG 0.745272 contig647end:-11266 MsG0080049070.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGGAAAGATGTCATATTAAT+CGG - contig647end:11282-11301 MsG0080049070.01.T01:CDS 25.0%
!! ATATATATTTTGCAGTTTGC+CGG - contig647end:11459-11478 MsG0080049070.01.T01:intron 25.0%
ATCATGCAACTATTCTTTCT+TGG - contig647end:11430-11449 MsG0080049070.01.T01:intron 30.0%
TCTTTCCTTGAATATCCAAA+TGG + contig647end:11270-11289 None:intergenic 30.0%
AAATGTTGTTCAACTTGTGC+TGG - contig647end:11503-11522 MsG0080049070.01.T01:intron 35.0%
GATGTCATATTAATCGGACT+TGG - contig647end:11288-11307 MsG0080049070.01.T01:CDS 35.0%
TATCTTCAGAGTCTCAAACA+AGG - contig647end:11560-11579 MsG0080049070.01.T01:CDS 35.0%
TGACTACGAAAGTAAAGTCA+TGG - contig647end:11608-11627 MsG0080049070.01.T01:CDS 35.0%
! ATTTTCCAATTACCACACAC+AGG - contig647end:11378-11397 MsG0080049070.01.T01:CDS 35.0%
ACAACATTTCCAGCTTGTAG+TGG + contig647end:11492-11511 None:intergenic 40.0%
AGGCACCATTTGGATATTCA+AGG - contig647end:11262-11281 MsG0080049070.01.T01:CDS 40.0%
CTACGAAAGTAAAGTCATGG+AGG - contig647end:11611-11630 MsG0080049070.01.T01:CDS 40.0%
CTTGGCATTGCCTTAAAATC+CGG - contig647end:11321-11340 MsG0080049070.01.T01:CDS 40.0%
! TACCTCAAGTCCGGATTTTA+AGG + contig647end:11334-11353 None:intergenic 40.0%
!! AGCTTGTAGTGGATCAAATC+CGG + contig647end:11481-11500 None:intergenic 40.0%
!!! GTTCAACTTGTGCTGGTTTT+GGG - contig647end:11510-11529 MsG0080049070.01.T01:intron 40.0%
!!! TGTTCAACTTGTGCTGGTTT+TGG - contig647end:11509-11528 MsG0080049070.01.T01:intron 40.0%
AAGTGCCTGTGTGTGGTAAT+TGG + contig647end:11386-11405 None:intergenic 45.0%
CAAACATAAGTGCCTGTGTG+TGG + contig647end:11393-11412 None:intergenic 45.0%
GAGTTGAGATACCTCAAGTC+CGG + contig647end:11343-11362 None:intergenic 45.0%
GGATTTGATCCACTACAAGC+TGG - contig647end:11480-11499 MsG0080049070.01.T01:intron 45.0%
TGCCTTAAAATCCGGACTTG+AGG - contig647end:11329-11348 MsG0080049070.01.T01:CDS 45.0%
TTGAGATCCATCCAACAGTG+AGG + contig647end:11536-11555 None:intergenic 45.0%
! GGACTTGGAGTTACTTTCCT+TGG - contig647end:11303-11322 MsG0080049070.01.T01:CDS 45.0%
!!! CGGATTTTAAGGCAATGCCA+AGG + contig647end:11323-11342 None:intergenic 45.0%
! TTTTGGGCCTCACTGTTGGA+TGG - contig647end:11526-11545 MsG0080049070.01.T01:intron 50.0%
!!! CTGGTTTTGGGCCTCACTGT+TGG - contig647end:11522-11541 MsG0080049070.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
contig647end gene 11258 11641 11258 ID=MsG0080049070.01;Name=MsG0080049070.01
contig647end mRNA 11258 11641 11258 ID=MsG0080049070.01.T01;Parent=MsG0080049070.01;Name=MsG0080049070.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|86
contig647end exon 11549 11641 11549 ID=MsG0080049070.01.T01:exon:8337;Parent=MsG0080049070.01.T01
contig647end exon 11258 11425 11258 ID=MsG0080049070.01.T01:exon:8338;Parent=MsG0080049070.01.T01
contig647end CDS 11549 11641 11549 ID=MsG0080049070.01.T01:cds;Parent=MsG0080049070.01.T01
contig647end CDS 11258 11425 11258 ID=MsG0080049070.01.T01:cds;Parent=MsG0080049070.01.T01
Gene Sequence

>MsG0080049070.01.T01

ACTAAGGCACCATTTGGATATTCAAGGAAAGATGTCATATTAATCGGACTTGGAGTTACTTTCCTTGGCATTGCCTTAAAATCCGGACTTGAGTTTGCCGGATTTGATCCACTACAAGCTGGAAATGTTGTTCAACTTGTGCTGGTTTTGGGCCTCACTGTTGGATGGATCTCAACATATATCTTCAGAGTCTCAAACAAGGAAATGACATATGCACAACAGCTACGTGACTACGAAAGTAAAGTCATGGAGGTAAAATAA

Protein sequence

>MsG0080049070.01.T01

TKAPFGYSRKDVILIGLGVTFLGIALKSGLEFAGFDPLQAGNVVQLVLVLGLTVGWISTYIFRVSNKEMTYAQQLRDYESKVMEVK*