AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023355.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023355.01.T01 MTR_8g091510 76.064 188 20 2 8 195 9 171 6.01e-94 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023355.01.T01 AT5G10070 71.094 128 26 1 68 195 44 160 1.39e-61 190
MsG0480023355.01.T01 AT5G10070 71.094 128 26 1 68 195 44 160 3.34e-61 192

Find 53 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCTGTTGACACAGGATTT+TGG 0.156208 4:+84145229 MsG0480023355.01.T01:CDS
CATGGCCGACCATAATTTAT+TGG 0.182712 4:-84147224 None:intergenic
GTATACATCACCGCCGAATT+TGG 0.230241 4:+84145184 MsG0480023355.01.T01:CDS
CAACAGCCAATCCTTTATTC+TGG 0.275842 4:-84146508 None:intergenic
TGGCTGCTGCTTTGACAATA+TGG 0.286533 4:+84147270 MsG0480023355.01.T01:CDS
TACTCTTTAATCCAGAATAA+AGG 0.315486 4:+84146497 MsG0480023355.01.T01:CDS
TTACGTGTGACTAATGGTTT+TGG 0.315588 4:+84146194 MsG0480023355.01.T01:CDS
TCTAATATCATGCATGTCAT+TGG 0.344335 4:+84145208 MsG0480023355.01.T01:CDS
GCGGCAAACCCAATAAATTA+TGG 0.347803 4:+84147215 MsG0480023355.01.T01:CDS
GCACAAGATATATCAGATTC+AGG 0.355976 4:-84146166 None:intergenic
ATGGCCGACCATAATTTATT+GGG 0.359883 4:-84147223 None:intergenic
CGGGCGCAAGCTTTCAAGAC+TGG 0.386858 4:+84145276 MsG0480023355.01.T01:CDS
GCGCAGCGCAACTTCACAAA+AGG 0.400711 4:-84146563 None:intergenic
GAAAATCAAAGCTGCGTTCA+AGG 0.401936 4:+84144746 MsG0480023355.01.T01:CDS
GCTATCTTGTGTAGAGGCCT+TGG 0.413021 4:+84147250 MsG0480023355.01.T01:CDS
GAGACACATTGCTTTCCAAC+TGG 0.415063 4:-84146464 None:intergenic
CTTCTTAATACTTTACTGTT+CGG 0.421998 4:-84144590 None:intergenic
CGACTGGATTGAGCATGATT+GGG 0.424602 4:-84144698 None:intergenic
GGGGCGATGATTTCTGAATC+TGG 0.426482 4:-84144678 None:intergenic
GATTAAAGAGTAATCTTCTC+TGG 0.428373 4:-84146486 None:intergenic
TCGACTGGATTGAGCATGAT+TGG 0.433538 4:-84144699 None:intergenic
TGAGAGTTGGTTGTGTCGAC+TGG 0.435345 4:-84144714 None:intergenic
GGGCGCAAGCTTTCAAGACT+GGG 0.452113 4:+84145277 MsG0480023355.01.T01:CDS
CATGATATTAGATCCAAATT+CGG 0.452530 4:-84145197 None:intergenic
TTGACAATATGGTATAGAAA+TGG 0.464235 4:+84147281 MsG0480023355.01.T01:CDS
CTAATATCATGCATGTCATT+GGG 0.467975 4:+84145209 MsG0480023355.01.T01:CDS
CAGGAGTTACGTGTGACTAA+TGG 0.482383 4:+84146188 MsG0480023355.01.T01:CDS
CTCCTCTAGAGTGGGAATAG+TGG 0.486456 4:-84145146 None:intergenic
TTTAATCCAGAATAAAGGAT+TGG 0.488019 4:+84146502 MsG0480023355.01.T01:CDS
GAATAAAGGATTGGCTGTTG+TGG 0.490897 4:+84146511 MsG0480023355.01.T01:CDS
GGCTGTTGTGGATTGCTCGT+GGG 0.496557 4:+84146523 MsG0480023355.01.T01:CDS
AATTGTTTGCGAGAGAGAGA+TGG 0.519701 4:+84144640 MsG0480023355.01.T01:exon
TAGAGTGGGAATAGTGGTTA+CGG 0.519867 4:-84145140 None:intergenic
CAGTCTTGAAAGCTTGCGCC+CGG 0.525147 4:-84145275 None:intergenic
ATTGTTTGCGAGAGAGAGAT+GGG 0.525702 4:+84144641 MsG0480023355.01.T01:exon
TTTCGTTGTCTAGTGAGAGT+TGG 0.547894 4:-84144727 None:intergenic
GGGTTTGCCGCTACAAGCCA+TGG 0.551853 4:-84147203 None:intergenic
TGGCTGTTGTGGATTGCTCG+TGG 0.556974 4:+84146522 MsG0480023355.01.T01:CDS
AGGATTTAGCTCCTCTAGAG+TGG 0.557390 4:-84145155 None:intergenic
GAATCTGATATATCTTGTGC+AGG 0.559064 4:+84146169 MsG0480023355.01.T01:CDS
TGCAGTGCCATGGCTTGTAG+CGG 0.560226 4:+84147196 MsG0480023355.01.T01:intron
TGTCATTGGGCTGTTGACAC+AGG 0.566948 4:+84145222 MsG0480023355.01.T01:CDS
TATTGTCAAAGCAGCAGCCA+AGG 0.583613 4:-84147267 None:intergenic
GCGGTGATGTATACAGTGGT+AGG 0.595033 4:-84145175 None:intergenic
ATGTCAGCTATCTTGTGTAG+AGG 0.595536 4:+84147244 MsG0480023355.01.T01:CDS
AACCACTATTCCCACTCTAG+AGG 0.628488 4:+84145144 MsG0480023355.01.T01:CDS
GGATTTAGCTCCTCTAGAGT+GGG 0.629875 4:-84145154 None:intergenic
CAAACCCAATAAATTATGGT+CGG 0.643896 4:+84147219 MsG0480023355.01.T01:CDS
GACTGGATTGAGCATGATTG+GGG 0.648452 4:-84144697 None:intergenic
TCTACACAAGATAGCTGACA+TGG 0.652062 4:-84147242 None:intergenic
TATAAGTTCATACAGAGGCG+AGG 0.658960 4:-84146590 None:intergenic
GATATTAGATCCAAATTCGG+CGG 0.670190 4:-84145194 None:intergenic
TTCGGCGGTGATGTATACAG+TGG 0.720667 4:-84145179 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTAGTTGCAAAATAT+TGG + Chr4:84145544-84145563 MsG0480023355.01.T01:intron 15.0%
!!! CAAAAAATTGATGATATAAA+AGG + Chr4:84146008-84146027 MsG0480023355.01.T01:intron 15.0%
!!! CATTATAATTATTTTTGACT+AGG + Chr4:84147304-84147323 MsG0480023355.01.T01:CDS 15.0%
!!! TGTTTTTTATACAATTTCTT+TGG + Chr4:84145960-84145979 MsG0480023355.01.T01:intron 15.0%
!!! TTTTGTTTACTTATATGAAT+GGG + Chr4:84145831-84145850 MsG0480023355.01.T01:intron 15.0%
!! AAATCTAATATTTCATATGG+AGG + Chr4:84145771-84145790 MsG0480023355.01.T01:intron 20.0%
!! ACAGTTAAATTATGTTCATA+AGG - Chr4:84144957-84144976 None:intergenic 20.0%
!! CTTATTATATAATATGTCAG+TGG + Chr4:84146142-84146161 MsG0480023355.01.T01:intron 20.0%
!! TCCAAATCTAATATTTCATA+TGG + Chr4:84145768-84145787 MsG0480023355.01.T01:intron 20.0%
!! TCCATATGAAATATTAGATT+TGG - Chr4:84145772-84145791 None:intergenic 20.0%
!! TCTTAAAAACGTTAATAAGA+AGG - Chr4:84146266-84146285 None:intergenic 20.0%
!!! AATTTCATGTATAGTTTTAG+AGG - Chr4:84145639-84145658 None:intergenic 20.0%
!!! AGATTAGTTTTGTTTTTTTC+TGG + Chr4:84145456-84145475 MsG0480023355.01.T01:intron 20.0%
!!! CAATATTTTGTTTTCTCTTT+TGG + Chr4:84146354-84146373 MsG0480023355.01.T01:intron 20.0%
!!! CTTTTGTTTACTTATATGAA+TGG + Chr4:84145830-84145849 MsG0480023355.01.T01:intron 20.0%
!!! TTAAAATAGTTTTGAATGCT+AGG + Chr4:84145500-84145519 MsG0480023355.01.T01:intron 20.0%
! AAACAAAATATTGCTTCACA+AGG - Chr4:84146347-84146366 None:intergenic 25.0%
! CATGATATTAGATCCAAATT+CGG - Chr4:84145200-84145219 None:intergenic 25.0%
! CATTACATTATTTGTGATTG+AGG - Chr4:84147164-84147183 None:intergenic 25.0%
! GTAAATGAATTGAAGAGTAA+TGG - Chr4:84144777-84144796 None:intergenic 25.0%
! GTGAAACAAATATCAATTAG+AGG - Chr4:84145591-84145610 None:intergenic 25.0%
! GTTGATTATGTAATCTATGA+AGG + Chr4:84146096-84146115 MsG0480023355.01.T01:intron 25.0%
! TACTCTTTAATCCAGAATAA+AGG + Chr4:84146497-84146516 MsG0480023355.01.T01:CDS 25.0%
! TTGACAATATGGTATAGAAA+TGG + Chr4:84147281-84147300 MsG0480023355.01.T01:CDS 25.0%
! TTTAATCCAGAATAAAGGAT+TGG + Chr4:84146502-84146521 MsG0480023355.01.T01:CDS 25.0%
!! AAATTGATGATATAAAAGGC+TGG + Chr4:84146012-84146031 MsG0480023355.01.T01:intron 25.0%
AAAATATTGGACGATCACAT+TGG + Chr4:84145557-84145576 MsG0480023355.01.T01:intron 30.0%
AATAACGTCGTGTTGAAATT+TGG + Chr4:84146749-84146768 MsG0480023355.01.T01:intron 30.0%
ACCACAGATTAAAGTAATTG+TGG - Chr4:84145889-84145908 None:intergenic 30.0%
ACTTTAATCTGTGGTAATAC+TGG + Chr4:84145894-84145913 MsG0480023355.01.T01:intron 30.0%
ATAACGTCGTGTTGAAATTT+GGG + Chr4:84146750-84146769 MsG0480023355.01.T01:intron 30.0%
ATCAAATTAATGCAGGTACA+AGG - Chr4:84145412-84145431 None:intergenic 30.0%
ATCATACTTGAAGTATGAAG+AGG + Chr4:84145714-84145733 MsG0480023355.01.T01:intron 30.0%
CAAACCCAATAAATTATGGT+CGG + Chr4:84147219-84147238 MsG0480023355.01.T01:CDS 30.0%
CAGATACTATGATATCTCTA+TGG - Chr4:84145921-84145940 None:intergenic 30.0%
CCACAGATTAAAGTAATTGT+GGG - Chr4:84145888-84145907 None:intergenic 30.0%
CTAATATCATGCATGTCATT+GGG + Chr4:84145209-84145228 MsG0480023355.01.T01:CDS 30.0%
CTCTTGAGAAGAGTTTATAA+AGG - Chr4:84146824-84146843 None:intergenic 30.0%
CTGATACCATGTTGAAATTT+GGG + Chr4:84146957-84146976 MsG0480023355.01.T01:intron 30.0%
CTTCAACATCAAATTAATGC+AGG - Chr4:84145419-84145438 None:intergenic 30.0%
GAGAAGAGTTTATAAAGATG+AGG - Chr4:84146819-84146838 None:intergenic 30.0%
GATTAAAGAGTAATCTTCTC+TGG - Chr4:84146489-84146508 None:intergenic 30.0%
GGCAAAAAGATTAAATGCAA+TGG - Chr4:84147128-84147147 None:intergenic 30.0%
GTTCCATAAAAATCCACAAT+CGG - Chr4:84145108-84145127 None:intergenic 30.0%
GTTTACTTATATGAATGGGT+AGG + Chr4:84145835-84145854 MsG0480023355.01.T01:intron 30.0%
TCAAATTAATGCAGGTACAA+GGG - Chr4:84145411-84145430 None:intergenic 30.0%
TCTAATATCATGCATGTCAT+TGG + Chr4:84145208-84145227 MsG0480023355.01.T01:CDS 30.0%
TCTGATACCATGTTGAAATT+TGG + Chr4:84146956-84146975 MsG0480023355.01.T01:intron 30.0%
TCTTTGGACAAAAAATCTAG+TGG + Chr4:84145976-84145995 MsG0480023355.01.T01:intron 30.0%
TGATACCATGTTGAAATTTG+GGG + Chr4:84146958-84146977 MsG0480023355.01.T01:intron 30.0%
TTGTGAGTGCAAATACATAT+TGG + Chr4:84145372-84145391 MsG0480023355.01.T01:intron 30.0%
! ATGTTCATAAGGTATCTTTG+AGG - Chr4:84144946-84144965 None:intergenic 30.0%
! ATTTTTATGGAACCCAAAGT+TGG + Chr4:84145115-84145134 MsG0480023355.01.T01:intron 30.0%
! GAAGAAATCTTTAAGTATCG+TGG - Chr4:84147100-84147119 None:intergenic 30.0%
! TAATCTTTTTGCCTCGTTTA+GGG + Chr4:84147135-84147154 MsG0480023355.01.T01:intron 30.0%
! TTAATCTTTTTGCCTCGTTT+AGG + Chr4:84147134-84147153 MsG0480023355.01.T01:intron 30.0%
AAATACTGTAGTGTAGAACC+AGG + Chr4:84146655-84146674 MsG0480023355.01.T01:intron 35.0%
AAGCGTATAAGTTCATACAG+AGG - Chr4:84146598-84146617 None:intergenic 35.0%
AGTAGTTATTCAGCGAACAA+AGG - Chr4:84146425-84146444 None:intergenic 35.0%
ATGGCCGACCATAATTTATT+GGG - Chr4:84147226-84147245 None:intergenic 35.0%
CAAAATCAAGCTTGCAATGT+GGG + Chr4:84144887-84144906 MsG0480023355.01.T01:intron 35.0%
CCCACAATTACTTTAATCTG+TGG + Chr4:84145885-84145904 MsG0480023355.01.T01:intron 35.0%
CTAACTCATCCTTACAAAAC+CGG + Chr4:84146775-84146794 MsG0480023355.01.T01:intron 35.0%
GAATCTGATATATCTTGTGC+AGG + Chr4:84146169-84146188 MsG0480023355.01.T01:CDS 35.0%
GAGAAGAGTTTATAAAGGTG+AGG - Chr4:84147026-84147045 None:intergenic 35.0%
GATATTAGATCCAAATTCGG+CGG - Chr4:84145197-84145216 None:intergenic 35.0%
GCACAAGATATATCAGATTC+AGG - Chr4:84146169-84146188 None:intergenic 35.0%
GCTTGTGTATAAGCTAACAA+AGG - Chr4:84144987-84145006 None:intergenic 35.0%
TAACGTCGTGTTGAAATTTG+GGG + Chr4:84146751-84146770 MsG0480023355.01.T01:intron 35.0%
TGATGATATAAAAGGCTGGT+TGG + Chr4:84146016-84146035 MsG0480023355.01.T01:intron 35.0%
TTTGTTTCACATGCTTTCAC+TGG + Chr4:84145601-84145620 MsG0480023355.01.T01:intron 35.0%
! ATAAAAGGCTGGTTGGTTAA+TGG + Chr4:84146023-84146042 MsG0480023355.01.T01:intron 35.0%
! CACATTGCAAGCTTGATTTT+GGG - Chr4:84144888-84144907 None:intergenic 35.0%
! TACGTGTGACTAATGGTTTT+GGG + Chr4:84146195-84146214 MsG0480023355.01.T01:CDS 35.0%
! TAGAGTTTTTGTAATGCCAC+AGG + Chr4:84145862-84145881 MsG0480023355.01.T01:intron 35.0%
! TCTCCGATTGTGGATTTTTA+TGG + Chr4:84145102-84145121 MsG0480023355.01.T01:intron 35.0%
! TTACGTGTGACTAATGGTTT+TGG + Chr4:84146194-84146213 MsG0480023355.01.T01:CDS 35.0%
! TTCTTTTTATTGCAGTGCCA+TGG + Chr4:84147186-84147205 MsG0480023355.01.T01:intron 35.0%
!! TTGTGTTTTTACAGTCCAGT+TGG + Chr4:84146449-84146468 MsG0480023355.01.T01:intron 35.0%
AACTTCACAGCTAGACGAAA+TGG + Chr4:84146306-84146325 MsG0480023355.01.T01:intron 40.0%
AATTGTTTGCGAGAGAGAGA+TGG + Chr4:84144640-84144659 MsG0480023355.01.T01:exon 40.0%
ACAATCGGAGATACTCTTAG+AGG - Chr4:84145093-84145112 None:intergenic 40.0%
ACGTTATTGCCAGATAGTAG+CGG - Chr4:84146737-84146756 None:intergenic 40.0%
ACTTCACAGCTAGACGAAAT+GGG + Chr4:84146307-84146326 MsG0480023355.01.T01:intron 40.0%
ATCAGAGCTTGTCGAATTCA+AGG - Chr4:84146942-84146961 None:intergenic 40.0%
ATGTCAGCTATCTTGTGTAG+AGG + Chr4:84147244-84147263 MsG0480023355.01.T01:CDS 40.0%
ATTGTTTGCGAGAGAGAGAT+GGG + Chr4:84144641-84144660 MsG0480023355.01.T01:exon 40.0%
CAACAGCCAATCCTTTATTC+TGG - Chr4:84146511-84146530 None:intergenic 40.0%
CATGGCCGACCATAATTTAT+TGG - Chr4:84147227-84147246 None:intergenic 40.0%
CCAAAATCAAGCTTGCAATG+TGG + Chr4:84144886-84144905 MsG0480023355.01.T01:intron 40.0%
GAAAATCAAAGCTGCGTTCA+AGG + Chr4:84144746-84144765 MsG0480023355.01.T01:CDS 40.0%
GCGGCAAACCCAATAAATTA+TGG + Chr4:84147215-84147234 MsG0480023355.01.T01:CDS 40.0%
GCTTTCACTGGAGTAATCTT+GGG + Chr4:84145613-84145632 MsG0480023355.01.T01:intron 40.0%
TAGAACCAGGTTGTCAATAG+CGG + Chr4:84146668-84146687 MsG0480023355.01.T01:intron 40.0%
TAGAGTGGGAATAGTGGTTA+CGG - Chr4:84145143-84145162 None:intergenic 40.0%
TATAAGTTCATACAGAGGCG+AGG - Chr4:84146593-84146612 None:intergenic 40.0%
TCAGAGCTTGTCGAATTCAA+GGG - Chr4:84146941-84146960 None:intergenic 40.0%
TCTACACAAGATAGCTGACA+TGG - Chr4:84147245-84147264 None:intergenic 40.0%
TCTTCATTATCACCCTCACA+AGG - Chr4:84144846-84144865 None:intergenic 40.0%
TGCTTTCACTGGAGTAATCT+TGG + Chr4:84145612-84145631 MsG0480023355.01.T01:intron 40.0%
TTAAAGTAATTGTGGGCCTG+TGG - Chr4:84145881-84145900 None:intergenic 40.0%
TTAGGCCCCAAATTTCAACA+TGG - Chr4:84146759-84146778 None:intergenic 40.0%
TTTCGTTGTCTAGTGAGAGT+TGG - Chr4:84144730-84144749 None:intergenic 40.0%
! ACGCTAAAGTAAGGCTTGTT+TGG - Chr4:84145060-84145079 None:intergenic 40.0%
! AGAGTCTTATCTATCCGATG+CGG + Chr4:84146840-84146859 MsG0480023355.01.T01:intron 40.0%
! AGAGTCTTATCTATCCGATG+TGG + Chr4:84146840-84146859 MsG0480023355.01.T01:intron 40.0%
! ATATGGAGGAAGCCTTTTGA+GGG + Chr4:84145785-84145804 MsG0480023355.01.T01:intron 40.0%
! CCACATTGCAAGCTTGATTT+TGG - Chr4:84144889-84144908 None:intergenic 40.0%
! CTTGGTGCATGAATAGGTTT+AGG + Chr4:84146912-84146931 MsG0480023355.01.T01:intron 40.0%
! GGGTGATAATGAAGAATCAG+AGG + Chr4:84144851-84144870 MsG0480023355.01.T01:intron 40.0%
! TGAAGTATGAAGAGGCATAG+AGG + Chr4:84145722-84145741 MsG0480023355.01.T01:intron 40.0%
!! ACGTGTGACTAATGGTTTTG+GGG + Chr4:84146196-84146215 MsG0480023355.01.T01:CDS 40.0%
!! CGGTTTTGTAAGGATGAGTT+AGG - Chr4:84146777-84146796 None:intergenic 40.0%
!! GAATAAAGGATTGGCTGTTG+TGG + Chr4:84146511-84146530 MsG0480023355.01.T01:CDS 40.0%
!! GGGAATTGCTTTAAGGTACT+TGG + Chr4:84146217-84146236 MsG0480023355.01.T01:intron 40.0%
!! TCTAAGAGTATCTCCGATTG+TGG + Chr4:84145092-84145111 MsG0480023355.01.T01:intron 40.0%
!!! GTTTTGGGGGAATTGCTTTA+AGG + Chr4:84146210-84146229 MsG0480023355.01.T01:CDS 40.0%
AACCACTATTCCCACTCTAG+AGG + Chr4:84145144-84145163 MsG0480023355.01.T01:CDS 45.0%
ACATGGTACCACTATTGCAG+CGG + Chr4:84146691-84146710 MsG0480023355.01.T01:intron 45.0%
AGTGGTTACGGAACCAACTT+TGG - Chr4:84145131-84145150 None:intergenic 45.0%
ATCGGAGATACTCTTAGAGG+AGG - Chr4:84145090-84145109 None:intergenic 45.0%
ATCTATCCGATGTGGCACTT+GGG + Chr4:84147055-84147074 MsG0480023355.01.T01:intron 45.0%
CAGGAGTTACGTGTGACTAA+TGG + Chr4:84146188-84146207 MsG0480023355.01.T01:CDS 45.0%
CGACTGGATTGAGCATGATT+GGG - Chr4:84144701-84144720 None:intergenic 45.0%
CTAACTGCAGAACCTTGTGA+GGG + Chr4:84144831-84144850 MsG0480023355.01.T01:intron 45.0%
GAAAAACCCAAGTGCCACAT+CGG - Chr4:84147064-84147083 None:intergenic 45.0%
GACTGGATTGAGCATGATTG+GGG - Chr4:84144700-84144719 None:intergenic 45.0%
GAGACACATTGCTTTCCAAC+TGG - Chr4:84146467-84146486 None:intergenic 45.0%
GGACGATCACATTGGATCAA+AGG + Chr4:84145565-84145584 MsG0480023355.01.T01:intron 45.0%
GGAGGCTAAACGCTAAAGTA+AGG - Chr4:84145069-84145088 None:intergenic 45.0%
GGTGCATGAATAGGTTTAGG+CGG + Chr4:84146915-84146934 MsG0480023355.01.T01:intron 45.0%
GTATACATCACCGCCGAATT+TGG + Chr4:84145184-84145203 MsG0480023355.01.T01:CDS 45.0%
GTGGTTACGGAACCAACTTT+GGG - Chr4:84145130-84145149 None:intergenic 45.0%
TATCTATCCGATGTGGCACT+TGG + Chr4:84147054-84147073 MsG0480023355.01.T01:intron 45.0%
TATTGTCAAAGCAGCAGCCA+AGG - Chr4:84147270-84147289 None:intergenic 45.0%
TCGACTGGATTGAGCATGAT+TGG - Chr4:84144702-84144721 None:intergenic 45.0%
TCTAACTGCAGAACCTTGTG+AGG + Chr4:84144830-84144849 MsG0480023355.01.T01:intron 45.0%
! AGGATTTAGCTCCTCTAGAG+TGG - Chr4:84145158-84145177 None:intergenic 45.0%
! CATATGGAGGAAGCCTTTTG+AGG + Chr4:84145784-84145803 MsG0480023355.01.T01:intron 45.0%
! GAGTCTTATCTATCCGATGC+GGG + Chr4:84146841-84146860 MsG0480023355.01.T01:intron 45.0%
! GGCTGTTGACACAGGATTTT+GGG + Chr4:84145230-84145249 MsG0480023355.01.T01:CDS 45.0%
! TGTGATGCCAAAAAGTGCAC+CGG + Chr4:84145256-84145275 MsG0480023355.01.T01:CDS 45.0%
!! CCTTACAAAACCGGCTTGTA+AGG + Chr4:84146784-84146803 MsG0480023355.01.T01:intron 45.0%
!! CCTTACAAGCCGGTTTTGTA+AGG - Chr4:84146787-84146806 None:intergenic 45.0%
!! GGATTTAGCTCCTCTAGAGT+GGG - Chr4:84145157-84145176 None:intergenic 45.0%
!! TGGCTGCTGCTTTGACAATA+TGG + Chr4:84147270-84147289 MsG0480023355.01.T01:CDS 45.0%
!! TTTGGGCTTGGTGCATGAAT+AGG + Chr4:84146906-84146925 MsG0480023355.01.T01:intron 45.0%
!!! CGTGTGACTAATGGTTTTGG+GGG + Chr4:84146197-84146216 MsG0480023355.01.T01:CDS 45.0%
AAGCCAAACCGCTGCAATAG+TGG - Chr4:84146702-84146721 None:intergenic 50.0%
AGTATCGTGGTGCGACGTAT+TGG - Chr4:84147087-84147106 None:intergenic 50.0%
ATCTATCCGATGCGGGACTT+GGG + Chr4:84146848-84146867 MsG0480023355.01.T01:intron 50.0%
CATGTCCGCTATTGACAACC+TGG - Chr4:84146676-84146695 None:intergenic 50.0%
CATGTTGCCTTGCCCTCAAA+AGG - Chr4:84145800-84145819 None:intergenic 50.0%
GAAAAACCCAAGTCCCGCAT+CGG - Chr4:84146857-84146876 None:intergenic 50.0%
GATTGAGGCAACCCTAAACG+AGG - Chr4:84147149-84147168 None:intergenic 50.0%
GCAATCCTCACCTTACAAGC+CGG - Chr4:84146797-84146816 None:intergenic 50.0%
GCGGTGATGTATACAGTGGT+AGG - Chr4:84145178-84145197 None:intergenic 50.0%
GCTATCTTGTGTAGAGGCCT+TGG + Chr4:84147250-84147269 MsG0480023355.01.T01:CDS 50.0%
GGAGATACTCTTAGAGGAGG+AGG - Chr4:84145087-84145106 None:intergenic 50.0%
GGGGCGATGATTTCTGAATC+TGG - Chr4:84144681-84144700 None:intergenic 50.0%
GTACCACTATTGCAGCGGTT+TGG + Chr4:84146696-84146715 MsG0480023355.01.T01:intron 50.0%
GTATCGTGGTGCGACGTATT+GGG - Chr4:84147086-84147105 None:intergenic 50.0%
TATCTATCCGATGCGGGACT+TGG + Chr4:84146847-84146866 MsG0480023355.01.T01:intron 50.0%
TGTCATTGGGCTGTTGACAC+AGG + Chr4:84145222-84145241 MsG0480023355.01.T01:CDS 50.0%
TTCGGCGGTGATGTATACAG+TGG - Chr4:84145182-84145201 None:intergenic 50.0%
! GGGCTGTTGACACAGGATTT+TGG + Chr4:84145229-84145248 MsG0480023355.01.T01:CDS 50.0%
! GTGATGCCAAAAAGTGCACC+GGG + Chr4:84145257-84145276 MsG0480023355.01.T01:CDS 50.0%
! TGAGAGTTGGTTGTGTCGAC+TGG - Chr4:84144717-84144736 None:intergenic 50.0%
!! CAAAACCGGCTTGTAAGGTG+AGG + Chr4:84146789-84146808 MsG0480023355.01.T01:intron 50.0%
!! CACCAAGCCCAAAGAGTGTT+GGG - Chr4:84146899-84146918 None:intergenic 50.0%
!! CAGGTTGTCAATAGCGGACA+TGG + Chr4:84146674-84146693 MsG0480023355.01.T01:intron 50.0%
!! CTCCTCTAGAGTGGGAATAG+TGG - Chr4:84145149-84145168 None:intergenic 50.0%
!! TATTGCAGCGGTTTGGCTTG+TGG + Chr4:84146703-84146722 MsG0480023355.01.T01:intron 50.0%
CAGTCTTGAAAGCTTGCGCC+CGG - Chr4:84145278-84145297 None:intergenic 55.0%
CCTCACGCCCAACACTCTTT+GGG + Chr4:84146889-84146908 MsG0480023355.01.T01:intron 55.0%
GCGCAGCGCAACTTCACAAA+AGG - Chr4:84146566-84146585 None:intergenic 55.0%
GGGCGCAAGCTTTCAAGACT+GGG + Chr4:84145277-84145296 MsG0480023355.01.T01:CDS 55.0%
! GCACCAAGCCCAAAGAGTGT+TGG - Chr4:84146900-84146919 None:intergenic 55.0%
! GGCTGTTGTGGATTGCTCGT+GGG + Chr4:84146523-84146542 MsG0480023355.01.T01:CDS 55.0%
! TGCAGTGCCATGGCTTGTAG+CGG + Chr4:84147196-84147215 MsG0480023355.01.T01:intron 55.0%
! TGGCTGTTGTGGATTGCTCG+TGG + Chr4:84146522-84146541 MsG0480023355.01.T01:CDS 55.0%
!! AAAGAGTGTTGGGCGTGAGG+GGG - Chr4:84146889-84146908 None:intergenic 55.0%
!! CAAAGAGTGTTGGGCGTGAG+GGG - Chr4:84146890-84146909 None:intergenic 55.0%
!! CCAAAGAGTGTTGGGCGTGA+GGG - Chr4:84146891-84146910 None:intergenic 55.0%
!! GAGGAAGCCTTTTGAGGGCA+AGG + Chr4:84145790-84145809 MsG0480023355.01.T01:intron 55.0%
CCCTCACGCCCAACACTCTT+TGG + Chr4:84146888-84146907 MsG0480023355.01.T01:intron 60.0%
CGCCCAACACTCTTTGGGCT+TGG + Chr4:84146894-84146913 MsG0480023355.01.T01:intron 60.0%
CGGGCGCAAGCTTTCAAGAC+TGG + Chr4:84145276-84145295 MsG0480023355.01.T01:CDS 60.0%
GGGTTTGCCGCTACAAGCCA+TGG - Chr4:84147206-84147225 None:intergenic 60.0%
! CTTGCGCCCGGTGCACTTTT+TGG - Chr4:84145266-84145285 None:intergenic 60.0%
! GTCGACTGGCCGCTACTATC+TGG + Chr4:84146725-84146744 MsG0480023355.01.T01:intron 60.0%
!! AAGAGTGTTGGGCGTGAGGG+GGG - Chr4:84146888-84146907 None:intergenic 60.0%
!! CCCAAAGAGTGTTGGGCGTG+AGG - Chr4:84146892-84146911 None:intergenic 60.0%
!! CGGTTTGGCTTGTGGTCGAC+TGG + Chr4:84146711-84146730 MsG0480023355.01.T01:intron 60.0%
! GGGCGTGAGGGGGGTGTATT+GGG - Chr4:84146879-84146898 None:intergenic 65.0%
! TGGGCGTGAGGGGGGTGTAT+TGG - Chr4:84146880-84146899 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 84144592 84147325 84144592 ID=MsG0480023355.01;Name=MsG0480023355.01
Chr4 mRNA 84144592 84147325 84144592 ID=MsG0480023355.01.T01;Parent=MsG0480023355.01;Name=MsG0480023355.01.T01;_AED=0.47;_eAED=0.59;_QI=67|0.5|0.4|1|0.5|0.4|5|0|206
Chr4 exon 84144592 84144767 84144592 ID=MsG0480023355.01.T01:exon:2623;Parent=MsG0480023355.01.T01
Chr4 exon 84145134 84145313 84145134 ID=MsG0480023355.01.T01:exon:2624;Parent=MsG0480023355.01.T01
Chr4 exon 84146162 84146231 84146162 ID=MsG0480023355.01.T01:exon:2625;Parent=MsG0480023355.01.T01
Chr4 exon 84146463 84146599 84146463 ID=MsG0480023355.01.T01:exon:2626;Parent=MsG0480023355.01.T01
Chr4 exon 84147201 84147325 84147201 ID=MsG0480023355.01.T01:exon:2627;Parent=MsG0480023355.01.T01
Chr4 five_prime_UTR 84144592 84144658 84144592 ID=MsG0480023355.01.T01:five_prime_utr;Parent=MsG0480023355.01.T01
Chr4 CDS 84144659 84144767 84144659 ID=MsG0480023355.01.T01:cds;Parent=MsG0480023355.01.T01
Chr4 CDS 84145134 84145313 84145134 ID=MsG0480023355.01.T01:cds;Parent=MsG0480023355.01.T01
Chr4 CDS 84146162 84146231 84146162 ID=MsG0480023355.01.T01:cds;Parent=MsG0480023355.01.T01
Chr4 CDS 84146463 84146599 84146463 ID=MsG0480023355.01.T01:cds;Parent=MsG0480023355.01.T01
Chr4 CDS 84147201 84147325 84147201 ID=MsG0480023355.01.T01:cds;Parent=MsG0480023355.01.T01
Gene Sequence

>MsG0480023355.01.T01

ATGGGCGTAATAACAGACACCAGATTCAGAAATCATCGCCCCAATCATGCTCAATCCAGTCGACACAACCAACTCTCACTAGACAACGAAAATCAAAGCTGCGTTCAAGTTGGTTCCGTAACCACTATTCCCACTCTAGAGGAGCTAAATCCTACCACTGTATACATCACCGCCGAATTTGGATCTAATATCATGCATGTCATTGGGCTGTTGACACAGGATTTTGGGCAATGTGATGCCAAAAAGTGCACCGGGCGCAAGCTTTCAAGACTGGGTTTTCTAAAAGCAATGGTCCTGAATCTGATATATCTTGTGCAGGAGTTACGTGTGACTAATGGTTTTGGGGGAATTGCTTTAAGTCCAGTTGGAAAGCAATGTGTCTCCAGAGAAGATTACTCTTTAATCCAGAATAAAGGATTGGCTGTTGTGGATTGCTCGTGGGCACGCTTAGATGACGTGCCTTTTGTGAAGTTGCGCTGCGCTGCTCCTCGCCTCTTGCCATGGCTTGTAGCGGCAAACCCAATAAATTATGGTCGGCCATGTCAGCTATCTTGTGTAGAGGCCTTGGCTGCTGCTTTGACAATATGGTATAGAAATGGCATTATAATTATTTTTGACTAG
Protein sequence

>MsG0480023355.01.T01

MGVITDTRFRNHRPNHAQSSRHNQLSLDNENQSCVQVGSVTTIPTLEELNPTTVYITAEFGSNIMHVIGLLTQDFGQCDAKKCTGRKLSRLGFLKAMVLNLIYLVQELRVTNGFGGIALSPVGKQCVSREDYSLIQNKGLAVVDCSWARLDDVPFVKLRCAAPRLLPWLVAANPINYGRPCQLSCVEALAAALTIWYRNGIIIIFD*