AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021706.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021706.01.T01 MTR_4g075100 64.286 112 19 2 14 107 127 235 3.44e-39 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021706.01.T01 AT5G23140 50.806 124 43 1 2 107 115 238 7.90e-34 118

Find 34 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTACCCTCCAATTCTAAT+TGG 0.195466 4:-63252551 None:intergenic
GCTCAAGAGGCAAAGGAGTT+TGG 0.292108 4:+63252449 MsG0480021706.01.T01:CDS
TCACAAGTGCTTATGCTCTT+AGG 0.332531 4:+63251969 MsG0480021706.01.T01:CDS
CTCAAGAGGCAAAGGAGTTT+GGG 0.337555 4:+63252450 MsG0480021706.01.T01:CDS
ATAAAGGTTCCAATTAGAAT+TGG 0.358203 4:+63252542 MsG0480021706.01.T01:CDS
ATCATGATTCACCAGCCTTC+TGG 0.360193 4:+63252279 MsG0480021706.01.T01:CDS
TGATCAGAGACCTATGACTT+TGG 0.371036 4:+63252490 MsG0480021706.01.T01:CDS
GCTGAAGAAGGCAAAGATAA+AGG 0.396103 4:+63252526 MsG0480021706.01.T01:CDS
TCTGTTGTGTGCAGGTGCCA+AGG 0.485472 4:+63252228 MsG0480021706.01.T01:intron
CACTGTACCCACCAGAAGGC+TGG 0.496466 4:-63252290 None:intergenic
ATAACATGATTCGTAATCAC+AGG 0.496646 4:-63252971 None:intergenic
TTCTGGTGGGTACAGTGGCC+AGG 0.499106 4:+63252296 MsG0480021706.01.T01:CDS
GAATTGCAATATCCTTTGCC+TGG 0.517374 4:-63252314 None:intergenic
AAGGTTCCAATTAGAATTGG+AGG 0.517580 4:+63252545 MsG0480021706.01.T01:CDS
TCTGATGCTGTTGCTGAAGA+AGG 0.521877 4:+63252514 MsG0480021706.01.T01:CDS
CAGCCTTCTGGTGGGTACAG+TGG 0.521919 4:+63252291 MsG0480021706.01.T01:CDS
CCAAGCAGATTGTTCGAATG+TGG 0.525727 4:+63252340 MsG0480021706.01.T01:CDS
TGAATCATGATTGTAGCATT+CGG 0.531675 4:-63252267 None:intergenic
GAGAGCACGCCTCTGAACCT+TGG 0.541022 4:-63252245 None:intergenic
GGAGTTTGGGATTATTGATG+AGG 0.549497 4:+63252463 MsG0480021706.01.T01:CDS
AGCATCAGAACCAAAGTCAT+AGG 0.552757 4:-63252500 None:intergenic
TTACTTTATGACTGCTCAAG+AGG 0.554775 4:+63252436 MsG0480021706.01.T01:CDS
TGGGTACAGTGGCCAGGCAA+AGG 0.561919 4:+63252302 MsG0480021706.01.T01:CDS
AATCATGTTATGAGATTGAG+AGG 0.562589 4:+63252983 MsG0480021706.01.T01:CDS
ATGATTCACCAGCCTTCTGG+TGG 0.571995 4:+63252282 MsG0480021706.01.T01:CDS
CCACATTCGAACAATCTGCT+TGG 0.578997 4:-63252340 None:intergenic
GTGCAGGTGCCAAGGTTCAG+AGG 0.585549 4:+63252236 MsG0480021706.01.T01:intron
TATGACTGCTCAAGAGGCAA+AGG 0.586598 4:+63252442 MsG0480021706.01.T01:CDS
CAAGCAGATTGTTCGAATGT+GGG 0.600409 4:+63252341 MsG0480021706.01.T01:CDS
TGGCCACTGTACCCACCAGA+AGG 0.601246 4:-63252294 None:intergenic
AGGTTCCAATTAGAATTGGA+GGG 0.604856 4:+63252546 MsG0480021706.01.T01:CDS
TGATTCACCAGCCTTCTGGT+GGG 0.661766 4:+63252283 MsG0480021706.01.T01:CDS
TAACATGATTCGTAATCACA+GGG 0.687071 4:-63252970 None:intergenic
GTGAAACACTGATATAGACA+CGG 0.713505 4:-63251943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAACAATCAGATATTATTA+TGG - Chr4:63252793-63252812 None:intergenic 20.0%
! AATTCAGAAGAATATGGATA+GGG + Chr4:63252412-63252431 MsG0480021706.01.T01:intron 25.0%
! ATAAAGGTTCCAATTAGAAT+TGG + Chr4:63252542-63252561 MsG0480021706.01.T01:CDS 25.0%
! GCAAACATTTAAATACAACA+AGG - Chr4:63252591-63252610 None:intergenic 25.0%
! TAAGTCCAAATAACTATTCA+CGG + Chr4:63251992-63252011 MsG0480021706.01.T01:intron 25.0%
! TAATTCAGAAGAATATGGAT+AGG + Chr4:63252411-63252430 MsG0480021706.01.T01:intron 25.0%
AATCATGTTATGAGATTGAG+AGG + Chr4:63252983-63253002 MsG0480021706.01.T01:CDS 30.0%
ATAACATGATTCGTAATCAC+AGG - Chr4:63252974-63252993 None:intergenic 30.0%
ATGATACTATGCAGTACATT+CGG + Chr4:63252156-63252175 MsG0480021706.01.T01:intron 30.0%
TAACATGATTCGTAATCACA+GGG - Chr4:63252973-63252992 None:intergenic 30.0%
TGAATCATGATTGTAGCATT+CGG - Chr4:63252270-63252289 None:intergenic 30.0%
TGACGTAATTCAGAAGAATA+TGG + Chr4:63252406-63252425 MsG0480021706.01.T01:intron 30.0%
!!! TATTTTGAGTTTTGATACCC+AGG + Chr4:63252877-63252896 MsG0480021706.01.T01:intron 30.0%
AAATGCAGCCTTGATCTAAT+GGG + Chr4:63252023-63252042 MsG0480021706.01.T01:intron 35.0%
AAGGTTCCAATTAGAATTGG+AGG + Chr4:63252545-63252564 MsG0480021706.01.T01:CDS 35.0%
AGGTTCCAATTAGAATTGGA+GGG + Chr4:63252546-63252565 MsG0480021706.01.T01:CDS 35.0%
ATTCATGACCCATTAGATCA+AGG - Chr4:63252034-63252053 None:intergenic 35.0%
ATTCTTCTGAATTACGTCAG+TGG - Chr4:63252405-63252424 None:intergenic 35.0%
GTGAAACACTGATATAGACA+CGG - Chr4:63251946-63251965 None:intergenic 35.0%
TGAACTGTGATCCTAACAAT+TGG + Chr4:63252102-63252121 MsG0480021706.01.T01:intron 35.0%
TTACTTTATGACTGCTCAAG+AGG + Chr4:63252436-63252455 MsG0480021706.01.T01:CDS 35.0%
TTGAGCCGTGAATAGTTATT+TGG - Chr4:63252000-63252019 None:intergenic 35.0%
!! TGCGCTCAATTAGGTTTTAT+TGG + Chr4:63252710-63252729 MsG0480021706.01.T01:intron 35.0%
ACTAGAAACATAGAGAGCCT+GGG - Chr4:63252897-63252916 None:intergenic 40.0%
AGCATCAGAACCAAAGTCAT+AGG - Chr4:63252503-63252522 None:intergenic 40.0%
ATCACTGCAACTAATGCCTA+AGG - Chr4:63252620-63252639 None:intergenic 40.0%
CAAATGCAGCCTTGATCTAA+TGG + Chr4:63252022-63252041 MsG0480021706.01.T01:intron 40.0%
CAAGCAGATTGTTCGAATGT+GGG + Chr4:63252341-63252360 MsG0480021706.01.T01:CDS 40.0%
CATTAGATCAAGGCTGCATT+TGG - Chr4:63252024-63252043 None:intergenic 40.0%
CCAATTAGAATTGGAGGGTA+AGG + Chr4:63252551-63252570 MsG0480021706.01.T01:intron 40.0%
CCGATCAACACAATCTGTTT+GGG + Chr4:63252182-63252201 MsG0480021706.01.T01:intron 40.0%
CCTTACCCTCCAATTCTAAT+TGG - Chr4:63252554-63252573 None:intergenic 40.0%
GAATTGCAATATCCTTTGCC+TGG - Chr4:63252317-63252336 None:intergenic 40.0%
GCTGAAGAAGGCAAAGATAA+AGG + Chr4:63252526-63252545 MsG0480021706.01.T01:CDS 40.0%
TCACAAGTGCTTATGCTCTT+AGG + Chr4:63251969-63251988 MsG0480021706.01.T01:CDS 40.0%
TCCGATCAACACAATCTGTT+TGG + Chr4:63252181-63252200 MsG0480021706.01.T01:intron 40.0%
TGATCAGAGACCTATGACTT+TGG + Chr4:63252490-63252509 MsG0480021706.01.T01:CDS 40.0%
TTCAGTCTTTGCGCTCAATT+AGG + Chr4:63252701-63252720 MsG0480021706.01.T01:intron 40.0%
TTCCAGTGACACCAATTGTT+AGG - Chr4:63252116-63252135 None:intergenic 40.0%
TTCTGAATTACGTCAGTGGT+TGG - Chr4:63252401-63252420 None:intergenic 40.0%
! CCCAAACAGATTGTGTTGAT+CGG - Chr4:63252185-63252204 None:intergenic 40.0%
! CTAATGGGTCATGAATCTGT+TGG + Chr4:63252038-63252057 MsG0480021706.01.T01:intron 40.0%
! GGAGTTTGGGATTATTGATG+AGG + Chr4:63252463-63252482 MsG0480021706.01.T01:CDS 40.0%
!! ATCCTAACAATTGGTGTCAC+TGG + Chr4:63252111-63252130 MsG0480021706.01.T01:intron 40.0%
ACACAACAGAAGAGAACCCA+TGG - Chr4:63252218-63252237 None:intergenic 45.0%
ACTGCAACTAATGCCTAAGG+CGG - Chr4:63252617-63252636 None:intergenic 45.0%
ATCATGATTCACCAGCCTTC+TGG + Chr4:63252279-63252298 MsG0480021706.01.T01:CDS 45.0%
CACTAGAAACATAGAGAGCC+TGG - Chr4:63252898-63252917 None:intergenic 45.0%
CCAAGCAGATTGTTCGAATG+TGG + Chr4:63252340-63252359 MsG0480021706.01.T01:CDS 45.0%
CCACATTCGAACAATCTGCT+TGG - Chr4:63252343-63252362 None:intergenic 45.0%
CTCAAGAGGCAAAGGAGTTT+GGG + Chr4:63252450-63252469 MsG0480021706.01.T01:CDS 45.0%
GCGCAAAGACTGAAGCTTAA+TGG - Chr4:63252695-63252714 None:intergenic 45.0%
TATGACTGCTCAAGAGGCAA+AGG + Chr4:63252442-63252461 MsG0480021706.01.T01:CDS 45.0%
TCTGATGCTGTTGCTGAAGA+AGG + Chr4:63252514-63252533 MsG0480021706.01.T01:CDS 45.0%
!! TGGTGTCACTGGAAATGTGT+AGG + Chr4:63252122-63252141 MsG0480021706.01.T01:intron 45.0%
AAATGTTTGCCTGCCGCCTT+AGG + Chr4:63252601-63252620 MsG0480021706.01.T01:intron 50.0%
ATGATTCACCAGCCTTCTGG+TGG + Chr4:63252282-63252301 MsG0480021706.01.T01:CDS 50.0%
GAGTTGTACGTCAAGCACAC+TGG + Chr4:63252375-63252394 MsG0480021706.01.T01:intron 50.0%
GCTCAAGAGGCAAAGGAGTT+TGG + Chr4:63252449-63252468 MsG0480021706.01.T01:CDS 50.0%
GGTTCTCTTCTGTTGTGTGC+AGG + Chr4:63252220-63252239 MsG0480021706.01.T01:intron 50.0%
TGATTCACCAGCCTTCTGGT+GGG + Chr4:63252283-63252302 MsG0480021706.01.T01:CDS 50.0%
CAACTAATGCCTAAGGCGGC+AGG - Chr4:63252613-63252632 None:intergenic 55.0%
TCTGTTGTGTGCAGGTGCCA+AGG + Chr4:63252228-63252247 MsG0480021706.01.T01:intron 55.0%
TTTGGGTCAGCTGCGTCCAT+GGG + Chr4:63252199-63252218 MsG0480021706.01.T01:intron 55.0%
CACTGTACCCACCAGAAGGC+TGG - Chr4:63252293-63252312 None:intergenic 60.0%
GTGCAGGTGCCAAGGTTCAG+AGG + Chr4:63252236-63252255 MsG0480021706.01.T01:intron 60.0%
GTTTGGGTCAGCTGCGTCCA+TGG + Chr4:63252198-63252217 MsG0480021706.01.T01:intron 60.0%
TGGCCACTGTACCCACCAGA+AGG - Chr4:63252297-63252316 None:intergenic 60.0%
TGGGTACAGTGGCCAGGCAA+AGG + Chr4:63252302-63252321 MsG0480021706.01.T01:CDS 60.0%
! CAGCCTTCTGGTGGGTACAG+TGG + Chr4:63252291-63252310 MsG0480021706.01.T01:CDS 60.0%
! GAGAGCACGCCTCTGAACCT+TGG - Chr4:63252248-63252267 None:intergenic 60.0%
! TTCTGGTGGGTACAGTGGCC+AGG + Chr4:63252296-63252315 MsG0480021706.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63251923 63253009 63251923 ID=MsG0480021706.01;Name=MsG0480021706.01
Chr4 mRNA 63251923 63253009 63251923 ID=MsG0480021706.01.T01;Parent=MsG0480021706.01;Name=MsG0480021706.01.T01;_AED=0.61;_eAED=0.64;_QI=0|0|0|0.5|1|1|4|0|130
Chr4 exon 63251923 63251990 63251923 ID=MsG0480021706.01.T01:exon:29277;Parent=MsG0480021706.01.T01
Chr4 exon 63252242 63252379 63252242 ID=MsG0480021706.01.T01:exon:29276;Parent=MsG0480021706.01.T01
Chr4 exon 63252433 63252567 63252433 ID=MsG0480021706.01.T01:exon:29275;Parent=MsG0480021706.01.T01
Chr4 exon 63252958 63253009 63252958 ID=MsG0480021706.01.T01:exon:29274;Parent=MsG0480021706.01.T01
Chr4 CDS 63251923 63251990 63251923 ID=MsG0480021706.01.T01:cds;Parent=MsG0480021706.01.T01
Chr4 CDS 63252242 63252379 63252242 ID=MsG0480021706.01.T01:cds;Parent=MsG0480021706.01.T01
Chr4 CDS 63252433 63252567 63252433 ID=MsG0480021706.01.T01:cds;Parent=MsG0480021706.01.T01
Chr4 CDS 63252958 63253009 63252958 ID=MsG0480021706.01.T01:cds;Parent=MsG0480021706.01.T01
Gene Sequence

>MsG0480021706.01.T01

ATGAGTGTTGTGTCTGTTATCCGTGTCTATATCAGTGTTTCACTGCTCACAAGTGCTTATGCTCTTAGGTGCCAAGGTTCAGAGGCGTGCTCTCCGAATGCTACAATCATGATTCACCAGCCTTCTGGTGGGTACAGTGGCCAGGCAAAGGATATTGCAATTCATACCAAGCAGATTGTTCGAATGTGGGATGCGCTGAATGAGTTGGATTACTTTATGACTGCTCAAGAGGCAAAGGAGTTTGGGATTATTGATGAGGTTATTGATCAGAGACCTATGACTTTGGTTCTGATGCTGTTGCTGAAGAAGGCAAAGATAAAGGTTCCAATTAGAATTGGAGGCATACTGATCAACCCTGTGATTACGAATCATGTTATGAGATTGAGAGGATGA

Protein sequence

>MsG0480021706.01.T01

MSVVSVIRVYISVSLLTSAYALRCQGSEACSPNATIMIHQPSGGYSGQAKDIAIHTKQIVRMWDALNELDYFMTAQEAKEFGIIDEVIDQRPMTLVLMLLLKKAKIKVPIRIGGILINPVITNHVMRLRG*