AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023357.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023357.01.T01 MTR_8g091510 68.966 145 6 1 10 154 168 273 1.41e-60 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023357.01.T01 AT5G10070 42.857 140 38 2 10 146 157 257 4.12e-23 92.0

Find 35 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGCTTGGCAAGTTTAAC+TGG 0.229226 4:+84147659 MsG0480023356.01.T01:CDS
AAATGATAAAGTAAATAATT+TGG 0.266881 4:-84149158 None:intergenic
CTCTAACCATGCTGCGATAC+TGG 0.303027 4:+84148915 MsG0480023356.01.T01:exon
TTATTGAAATGAAAACTATT+TGG 0.307741 4:+84147587 MsG0480023356.01.T01:intron
AAATAATTTCAGCACTGTTT+TGG 0.333601 4:-84148467 None:intergenic
TTATTTCTGTTCAAAATGAT+TGG 0.353309 4:+84148484 MsG0480023356.01.T01:CDS
CTGCGATACTGGAATCAATA+TGG 0.360424 4:+84148926 MsG0480023356.01.T01:exon
CTCTTCATCACTATTGTCTA+TGG 0.381809 4:-84148854 None:intergenic
AACTCTTCCGATTCTGATGA+TGG 0.381819 4:+84148796 MsG0480023356.01.T01:exon
TCACTATCAGTAGGAGCCTT+TGG 0.382863 4:-84148528 None:intergenic
CCTTCAGTAATTCCCTGTAT+TGG 0.387471 4:-84148431 None:intergenic
TTGGCTATCACAAGCGCGTC+AGG 0.460396 4:+84148503 MsG0480023356.01.T01:CDS
AGCTGAGATATGCATCGTCT+TGG 0.461211 4:+84148989 MsG0480023356.01.T01:exon
GTTGCTTGGCAAGTTTAACT+GGG 0.464029 4:+84147660 MsG0480023356.01.T01:CDS
GCTGAGATATGCATCGTCTT+GGG 0.486978 4:+84148990 MsG0480023356.01.T01:exon
TATGCTGCATGGCAGTTGTT+AGG 0.502876 4:+84148749 MsG0480023356.01.T01:intron
TGGAAGCCCATCATCAGAAT+CGG 0.511595 4:-84148803 None:intergenic
TTCTTTGCTATATCTTGCAG+TGG 0.518716 4:+84147616 MsG0480023356.01.T01:intron
GAAACAGCAAATCTGTTGCT+TGG 0.521180 4:+84147646 MsG0480023356.01.T01:CDS
TTTCACTTATGATATTTACA+AGG 0.521917 4:+84147764 MsG0480023356.01.T01:intron
ATGGGCTTCCACCTCTTGAA+AGG 0.540522 4:+84148815 MsG0480023356.01.T01:exon
TTCATGTTCCTTTCAAGAGG+TGG 0.548622 4:-84148823 None:intergenic
ACTCTTCCGATTCTGATGAT+GGG 0.551069 4:+84148797 MsG0480023356.01.T01:exon
TGATTCATGTTCCTTTCAAG+AGG 0.557002 4:-84148826 None:intergenic
TTCACTTATGATATTTACAA+GGG 0.562228 4:+84147765 MsG0480023356.01.T01:intron
TGAAAATTACAGAAACATCA+AGG 0.577569 4:-84149113 None:intergenic
TTAATGTATAAGAGAAACGC+TGG 0.584564 4:-84149026 None:intergenic
TTGCTTGGCAAGTTTAACTG+GGG 0.613832 4:+84147661 MsG0480023356.01.T01:CDS
AAACATCAAGGCCTCACAGA+GGG 0.624939 4:-84149101 None:intergenic
CCAATACAGGGAATTACTGA+AGG 0.625012 4:+84148431 MsG0480023356.01.T01:intron
ACAAGCGCGTCAGGTTCCAA+AGG 0.626008 4:+84148512 MsG0480023356.01.T01:CDS
TTGATTCCAGTATCGCAGCA+TGG 0.640776 4:-84148921 None:intergenic
CACAGACCTTCACTATCAGT+AGG 0.664914 4:-84148537 None:intergenic
AAGGCTCCTACTGATAGTGA+AGG 0.667476 4:+84148531 MsG0480023356.01.T01:CDS
GAAACATCAAGGCCTCACAG+AGG 0.751301 4:-84149102 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTGATTCTCAATTATAT+GGG + Chr4:84148370-84148389 MsG0480023356.01.T01:intron 15.0%
!! ATATAATTGAGAATCAAATT+CGG - Chr4:84148370-84148389 None:intergenic 15.0%
!!! TGTTATATATCTATTTTACA+CGG + Chr4:84148562-84148581 MsG0480023356.01.T01:intron 15.0%
!!! TTAACAAAAAAATTTGTTCA+TGG - Chr4:84148034-84148053 None:intergenic 15.0%
!! AAAAATTCACAATATATCAG+AGG - Chr4:84148707-84148726 None:intergenic 20.0%
!! ATTTGATTCTCAATTATATG+GGG + Chr4:84148371-84148390 MsG0480023356.01.T01:intron 20.0%
!! CTTATAAATATGCTTCATAA+AGG - Chr4:84147947-84147966 None:intergenic 20.0%
!! GAATTTGATTCTCAATTATA+TGG + Chr4:84148369-84148388 MsG0480023356.01.T01:intron 20.0%
!! TAGTTATGTATAGATAACTA+GGG + Chr4:84148088-84148107 MsG0480023356.01.T01:intron 20.0%
!! TTAGTTATGTATAGATAACT+AGG + Chr4:84148087-84148106 MsG0480023356.01.T01:intron 20.0%
!! TTCACTTATGATATTTACAA+GGG + Chr4:84147765-84147784 MsG0480023356.01.T01:intron 20.0%
!! TTTCACTTATGATATTTACA+AGG + Chr4:84147764-84147783 MsG0480023356.01.T01:intron 20.0%
!!! GTTATATATCTATTTTACAC+GGG + Chr4:84148563-84148582 MsG0480023356.01.T01:intron 20.0%
!!! TTATATATCTATTTTACACG+GGG + Chr4:84148564-84148583 MsG0480023356.01.T01:intron 20.0%
!!! TTATTTCTGTTCAAAATGAT+TGG + Chr4:84148484-84148503 MsG0480023356.01.T01:CDS 20.0%
!!! TTTTTATATCATGAACTACA+AGG - Chr4:84147886-84147905 None:intergenic 20.0%
! AAGTTATGTAAGTTACGTTA+CGG - Chr4:84147915-84147934 None:intergenic 25.0%
! CATATTAGATCTCTCAATTA+AGG - Chr4:84148199-84148218 None:intergenic 25.0%
! GTTAAATGAAAAAAGCAACA+GGG + Chr4:84147830-84147849 MsG0480023356.01.T01:intron 25.0%
! TAATTCCAGTATTCATTTGT+AGG - Chr4:84147992-84148011 None:intergenic 25.0%
! TGAAAATTACAGAAACATCA+AGG - Chr4:84149116-84149135 None:intergenic 25.0%
! TGTTAAATGAAAAAAGCAAC+AGG + Chr4:84147829-84147848 MsG0480023356.01.T01:intron 25.0%
!! AAATAATTTCAGCACTGTTT+TGG - Chr4:84148470-84148489 None:intergenic 25.0%
!! AACTTTCTTTTAACCAATAC+AGG + Chr4:84148418-84148437 MsG0480023356.01.T01:intron 25.0%
!! ACTTTCTTTTAACCAATACA+GGG + Chr4:84148419-84148438 MsG0480023356.01.T01:intron 25.0%
!!! ATATTTTCACTTTGCTATCT+AGG + Chr4:84147737-84147756 MsG0480023356.01.T01:intron 25.0%
!!! ATTTGTTTTTAATCTGACAC+TGG + Chr4:84148886-84148905 MsG0480023356.01.T01:exon 25.0%
!!! CTTTTTTCATTTAACAGTGT+GGG - Chr4:84147825-84147844 None:intergenic 25.0%
ATCAGATACAAGAGTAATCA+AGG - Chr4:84148166-84148185 None:intergenic 30.0%
CGAAACAATTGGAATGAATT+TGG - Chr4:84148314-84148333 None:intergenic 30.0%
TAGATAAAAGATTGAACGCA+TGG - Chr4:84148614-84148633 None:intergenic 30.0%
TCAGATACAAGAGTAATCAA+GGG - Chr4:84148165-84148184 None:intergenic 30.0%
TTAATGTATAAGAGAAACGC+TGG - Chr4:84149029-84149048 None:intergenic 30.0%
TTTCATGGAAACGAAACAAT+TGG - Chr4:84148325-84148344 None:intergenic 30.0%
! GAATGAATTTGGTTCACAAT+TGG - Chr4:84148303-84148322 None:intergenic 30.0%
!!! GCTTTTTTCATTTAACAGTG+TGG - Chr4:84147826-84147845 None:intergenic 30.0%
AAGGTTCGAAAGAAATCAAG+AGG - Chr4:84147867-84147886 None:intergenic 35.0%
AGGTTCGAAAGAAATCAAGA+GGG - Chr4:84147866-84147885 None:intergenic 35.0%
CAGATACAAGAGTAATCAAG+GGG - Chr4:84148164-84148183 None:intergenic 35.0%
CAGTACCTACAAATGAATAC+TGG + Chr4:84147984-84148003 MsG0480023356.01.T01:intron 35.0%
CTCTTCATCACTATTGTCTA+TGG - Chr4:84148857-84148876 None:intergenic 35.0%
CTGTGAACTAAAGGAGTTTA+TGG + Chr4:84148268-84148287 MsG0480023356.01.T01:intron 35.0%
TGATTCATGTTCCTTTCAAG+AGG - Chr4:84148829-84148848 None:intergenic 35.0%
! TAAACTCCTTTAGTTCACAG+TGG - Chr4:84148268-84148287 None:intergenic 35.0%
! TTCTTTGCTATATCTTGCAG+TGG + Chr4:84147616-84147635 MsG0480023356.01.T01:intron 35.0%
!!! ACTGTTTTGGCATTTAGAGT+AGG - Chr4:84148457-84148476 None:intergenic 35.0%
AAAGAAATCAAGAGGGACTC+TGG - Chr4:84147859-84147878 None:intergenic 40.0%
AACTCTTCCGATTCTGATGA+TGG + Chr4:84148796-84148815 MsG0480023356.01.T01:exon 40.0%
AAGAAATCAAGAGGGACTCT+GGG - Chr4:84147858-84147877 None:intergenic 40.0%
ACTCTTCCGATTCTGATGAT+GGG + Chr4:84148797-84148816 MsG0480023356.01.T01:exon 40.0%
CATGTTAGACACGGATTTCA+TGG - Chr4:84148340-84148359 None:intergenic 40.0%
CCAATACAGGGAATTACTGA+AGG + Chr4:84148431-84148450 MsG0480023356.01.T01:intron 40.0%
CCTAACAATCATAGCTAGTG+AGG - Chr4:84148114-84148133 None:intergenic 40.0%
CCTCACTAGCTATGATTGTT+AGG + Chr4:84148111-84148130 MsG0480023356.01.T01:intron 40.0%
CCTTCAGTAATTCCCTGTAT+TGG - Chr4:84148434-84148453 None:intergenic 40.0%
CTGCGATACTGGAATCAATA+TGG + Chr4:84148926-84148945 MsG0480023356.01.T01:exon 40.0%
GAAACAGCAAATCTGTTGCT+TGG + Chr4:84147646-84147665 MsG0480023356.01.T01:CDS 40.0%
GTACAGTGAAATTGTGTGCT+AGG - Chr4:84148225-84148244 None:intergenic 40.0%
GTGAAACCACTGTGAACTAA+AGG + Chr4:84148259-84148278 MsG0480023356.01.T01:intron 40.0%
GTTGCTTGGCAAGTTTAACT+GGG + Chr4:84147660-84147679 MsG0480023356.01.T01:CDS 40.0%
TTCATGGCTACATACAGAGA+TGG - Chr4:84148018-84148037 None:intergenic 40.0%
TTCATGTTCCTTTCAAGAGG+TGG - Chr4:84148826-84148845 None:intergenic 40.0%
TTGCTTGGCAAGTTTAACTG+GGG + Chr4:84147661-84147680 MsG0480023356.01.T01:CDS 40.0%
! ACATGTATTTTCCCTCTGTG+AGG + Chr4:84149090-84149109 MsG0480023356.01.T01:exon 40.0%
! TGTTGCTTGGCAAGTTTAAC+TGG + Chr4:84147659-84147678 MsG0480023356.01.T01:CDS 40.0%
!! CTATTTTACACGGGGTGTTA+TGG + Chr4:84148572-84148591 MsG0480023356.01.T01:intron 40.0%
!!! TTTTTTCCTCCTATGCTGCA+TGG + Chr4:84148738-84148757 MsG0480023356.01.T01:intron 40.0%
AAACATCAAGGCCTCACAGA+GGG - Chr4:84149104-84149123 None:intergenic 45.0%
AGCTGAGATATGCATCGTCT+TGG + Chr4:84148989-84149008 MsG0480023356.01.T01:exon 45.0%
CACAGACCTTCACTATCAGT+AGG - Chr4:84148540-84148559 None:intergenic 45.0%
GAGTAATCAAGGGGCTAATC+AGG - Chr4:84148155-84148174 None:intergenic 45.0%
GCTGAGATATGCATCGTCTT+GGG + Chr4:84148990-84149009 MsG0480023356.01.T01:exon 45.0%
GGTGAATCGCATGTTAGACA+CGG - Chr4:84148349-84148368 None:intergenic 45.0%
GTTGCATACATTGTCTGTGC+AGG + Chr4:84148652-84148671 MsG0480023356.01.T01:intron 45.0%
TAACAACTGCCATGCAGCAT+AGG - Chr4:84148750-84148769 None:intergenic 45.0%
TATGCTGCATGGCAGTTGTT+AGG + Chr4:84148749-84148768 MsG0480023356.01.T01:intron 45.0%
TCACTATCAGTAGGAGCCTT+TGG - Chr4:84148531-84148550 None:intergenic 45.0%
TGGAAGCCCATCATCAGAAT+CGG - Chr4:84148806-84148825 None:intergenic 45.0%
TTGATTCCAGTATCGCAGCA+TGG - Chr4:84148924-84148943 None:intergenic 45.0%
! AAGGCTCCTACTGATAGTGA+AGG + Chr4:84148531-84148550 MsG0480023356.01.T01:CDS 45.0%
! GGGGTGTTATGGTATGAAGT+TGG + Chr4:84148583-84148602 MsG0480023356.01.T01:intron 45.0%
AGGGGCTAATCAGGAGCTAA+TGG - Chr4:84148146-84148165 None:intergenic 50.0%
ATGGGCTTCCACCTCTTGAA+AGG + Chr4:84148815-84148834 MsG0480023356.01.T01:exon 50.0%
CTCTAACCATGCTGCGATAC+TGG + Chr4:84148915-84148934 MsG0480023356.01.T01:exon 50.0%
GAAACATCAAGGCCTCACAG+AGG - Chr4:84149105-84149124 None:intergenic 50.0%
ACAAGCGCGTCAGGTTCCAA+AGG + Chr4:84148512-84148531 MsG0480023356.01.T01:CDS 55.0%
CAACTGCCATGCAGCATAGG+AGG - Chr4:84148747-84148766 None:intergenic 55.0%
TTGGCTATCACAAGCGCGTC+AGG + Chr4:84148503-84148522 MsG0480023356.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 84147595 84149168 84147595 ID=MsG0480023357.01;Name=MsG0480023357.01
Chr4 mRNA 84147595 84149168 84147595 ID=MsG0480023357.01.T01;Parent=MsG0480023357.01;Name=MsG0480023357.01.T01;_AED=0.46;_eAED=0.55;_QI=0|0.5|0.33|1|0.5|0.33|3|283|154
Chr4 exon 84147595 84147786 84147595 ID=MsG0480023357.01.T01:exon:2628;Parent=MsG0480023357.01.T01
Chr4 exon 84148402 84148552 84148402 ID=MsG0480023357.01.T01:exon:2629;Parent=MsG0480023357.01.T01
Chr4 exon 84148764 84149168 84148764 ID=MsG0480023357.01.T01:exon:2630;Parent=MsG0480023357.01.T01
Chr4 CDS 84147595 84147786 84147595 ID=MsG0480023357.01.T01:cds;Parent=MsG0480023357.01.T01
Chr4 CDS 84148402 84148552 84148402 ID=MsG0480023357.01.T01:cds;Parent=MsG0480023357.01.T01
Chr4 CDS 84148764 84148885 84148764 ID=MsG0480023357.01.T01:cds;Parent=MsG0480023357.01.T01
Chr4 three_prime_UTR 84148886 84149168 84148886 ID=MsG0480023357.01.T01:three_prime_utr;Parent=MsG0480023357.01.T01
Gene Sequence

>MsG0480023357.01.T01

ATGAAAACTATTTGGTTGAAGTTCTTTGCTATATCTTGCAGTGGAGAAGAAGAAACAGCAAATCTGTTGCTTGGCAAGTTTAACTGGGGTCACGCTTTTTCGTCTCTGAATAAGTATGATATGATTCATTTATTTAATTTTTATATTTTCACTTTGCTATCTAGGTTATTTTCACTTATGATATTTACAAGGACTTTTCTATATTCAGAACTTTCTTTTAACCAATACAGGGAATTACTGAAGGCCTACTCTAAATGCCAAAACAGTGCTGAAATTATTTCTGTTCAAAATGATTGGCTATCACAAGCGCGTCAGGTTCCAAAGGCTCCTACTGATAGTGAAGTTGTTAGGCAAGAAAATGAAGATAATGATCAAAACTCTTCCGATTCTGATGATGGGCTTCCACCTCTTGAAAGGAACATGAATCATTTGACCATAGACAATAGTGATGAAGAGAGTGAGTAG

Protein sequence

>MsG0480023357.01.T01

MKTIWLKFFAISCSGEEETANLLLGKFNWGHAFSSLNKYDMIHLFNFYIFTLLSRLFSLMIFTRTFLYSELSFNQYRELLKAYSKCQNSAEIISVQNDWLSQARQVPKAPTDSEVVRQENEDNDQNSSDSDDGLPPLERNMNHLTIDNSDEESE*