Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023359.01.T01 | AET04560.1 | 94.444 | 144 | 8 | 0 | 1 | 144 | 1 | 144 | 5.81E-94 | 281 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023359.01.T01 | Q9ZT50 | 54.386 | 57 | 24 | 1 | 67 | 123 | 85 | 139 | 5.13E-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023359.01.T01 | G7LAG1 | 94.444 | 144 | 8 | 0 | 1 | 144 | 1 | 144 | 2.78e-94 | 281 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380011496.01 | MsG0480023359.01 | 0.800542 | 1.372407e-48 | 6.407128e-46 |
| MsG0380011501.01 | MsG0480023359.01 | 0.808705 | 2.715412e-50 | 1.563425e-47 |
| MsG0480023359.01 | MsG0580027925.01 | 0.808810 | 2.579317e-50 | 1.489155e-47 |
| MsG0280007955.01 | MsG0480023359.01 | 0.807637 | 4.585923e-50 | 2.567499e-47 |
| MsG0280008361.01 | MsG0480023359.01 | 0.801436 | 9.012276e-49 | 4.303711e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023359.01.T01 | MTR_8g091560 | 94.444 | 144 | 8 | 0 | 1 | 144 | 1 | 144 | 7.04e-98 | 281 |
| MsG0480023359.01.T01 | MTR_3g087900 | 49.660 | 147 | 70 | 3 | 1 | 145 | 1 | 145 | 6.16e-41 | 134 |
| MsG0480023359.01.T01 | MTR_2g096350 | 41.304 | 92 | 44 | 5 | 54 | 138 | 117 | 205 | 2.98e-14 | 67.4 |
| MsG0480023359.01.T01 | MTR_8g098770 | 34.559 | 136 | 71 | 5 | 5 | 124 | 8 | 141 | 4.73e-14 | 65.9 |
| MsG0480023359.01.T01 | MTR_5g026260 | 42.647 | 68 | 36 | 3 | 57 | 123 | 494 | 559 | 8.08e-13 | 65.1 |
| MsG0480023359.01.T01 | MTR_5g066690 | 42.500 | 80 | 39 | 2 | 33 | 112 | 29 | 101 | 8.98e-13 | 61.6 |
| MsG0480023359.01.T01 | MTR_4g063070 | 50.000 | 58 | 25 | 2 | 66 | 122 | 334 | 388 | 1.34e-12 | 64.3 |
| MsG0480023359.01.T01 | MTR_5g066700 | 50.000 | 54 | 25 | 1 | 62 | 115 | 53 | 104 | 2.67e-12 | 60.5 |
| MsG0480023359.01.T01 | MTR_4g077570 | 51.923 | 52 | 25 | 0 | 64 | 115 | 7 | 58 | 5.87e-12 | 58.2 |
| MsG0480023359.01.T01 | MTR_0010s0050 | 35.227 | 88 | 44 | 1 | 30 | 117 | 49 | 123 | 1.20e-11 | 58.9 |
| MsG0480023359.01.T01 | MTR_4g120270 | 52.830 | 53 | 21 | 2 | 64 | 115 | 100 | 149 | 1.65e-11 | 60.5 |
| MsG0480023359.01.T01 | MTR_4g116460 | 50.000 | 48 | 22 | 2 | 68 | 115 | 441 | 486 | 1.78e-11 | 60.8 |
| MsG0480023359.01.T01 | MTR_4g116460 | 50.000 | 48 | 22 | 2 | 68 | 115 | 484 | 529 | 1.81e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023359.01.T01 | AT1G15100 | 54.386 | 57 | 24 | 1 | 67 | 123 | 85 | 139 | 5.23e-15 | 68.2 |
| MsG0480023359.01.T01 | AT5G45290 | 48.214 | 56 | 28 | 1 | 68 | 123 | 490 | 544 | 7.48e-14 | 67.8 |
| MsG0480023359.01.T01 | AT3G16720 | 56.364 | 55 | 20 | 2 | 64 | 117 | 115 | 166 | 1.86e-12 | 63.5 |
| MsG0480023359.01.T01 | AT5G45290 | 48.214 | 56 | 27 | 2 | 68 | 123 | 490 | 543 | 2.27e-12 | 63.5 |
| MsG0480023359.01.T01 | AT5G41400 | 46.552 | 58 | 29 | 2 | 68 | 124 | 105 | 161 | 1.55e-11 | 59.3 |
| MsG0480023359.01.T01 | AT4G00305 | 41.538 | 65 | 31 | 3 | 55 | 118 | 62 | 120 | 2.14e-11 | 58.2 |
| MsG0480023359.01.T01 | AT1G72310 | 45.902 | 61 | 29 | 2 | 64 | 123 | 123 | 180 | 2.29e-11 | 60.5 |
| MsG0480023359.01.T01 | AT2G20030 | 46.875 | 64 | 29 | 3 | 60 | 121 | 116 | 176 | 4.69e-11 | 59.7 |
| MsG0480023359.01.T01 | AT4G28890 | 45.312 | 64 | 31 | 2 | 60 | 122 | 115 | 175 | 4.86e-11 | 59.7 |
| MsG0480023359.01.T01 | AT1G23980 | 50.847 | 59 | 25 | 2 | 60 | 117 | 136 | 191 | 6.00e-11 | 59.3 |
| MsG0480023359.01.T01 | AT4G30400 | 55.319 | 47 | 17 | 2 | 67 | 112 | 133 | 176 | 7.10e-11 | 59.3 |
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTACTGGGTTGTGGTAAT+TGG | 0.239689 | 4:-84160805 | None:intergenic |
| GTGCTACTTGTCCACTTTGC+AGG | 0.272706 | 4:+84161004 | MsG0480023359.01.T01:CDS |
| CACATTTCATAAGGATTGTT+TGG | 0.286072 | 4:+84160954 | MsG0480023359.01.T01:CDS |
| TTGAGGAAGGGGACATTGTT+AGG | 0.300056 | 4:+84160914 | MsG0480023359.01.T01:CDS |
| ATAAGGATTGTTTGGACAAA+TGG | 0.327903 | 4:+84160962 | MsG0480023359.01.T01:CDS |
| CTTCAATGAAATTGAGGTAC+TGG | 0.330326 | 4:-84160819 | None:intergenic |
| CGATCAGTATATATGTAAAT+AGG | 0.332800 | 4:-84160741 | None:intergenic |
| GTATTGTTTGCTTCAAAATC+AGG | 0.367220 | 4:+84161059 | MsG0480023359.01.T01:CDS |
| CAAAATCAGGTAGAATTTGA+TGG | 0.399273 | 4:+84161072 | MsG0480023359.01.T01:CDS |
| GGCTTCTTCAACCTTCTAGT+GGG | 0.406833 | 4:-84160850 | None:intergenic |
| CAATCTATGTGCTCTGCTGC+AGG | 0.424192 | 4:-84160871 | None:intergenic |
| CTGTGAACACACATTTCATA+AGG | 0.434557 | 4:+84160945 | MsG0480023359.01.T01:CDS |
| TCTTCTCTTCAATGAAATTG+AGG | 0.435803 | 4:-84160825 | None:intergenic |
| TGTTTATCTGAGTTTGAGGA+AGG | 0.453729 | 4:+84160901 | MsG0480023359.01.T01:CDS |
| CAGCAGAGCACATAGATTGT+AGG | 0.454258 | 4:+84160875 | MsG0480023359.01.T01:CDS |
| GGTATGTTTATCTGAGTTTG+AGG | 0.460224 | 4:+84160897 | MsG0480023359.01.T01:CDS |
| TTTATCTGAGTTTGAGGAAG+GGG | 0.469479 | 4:+84160903 | MsG0480023359.01.T01:CDS |
| AAGAGAAGAACCCCACTAGA+AGG | 0.481865 | 4:+84160839 | MsG0480023359.01.T01:CDS |
| AAAATCAGGTAGAATTTGAT+GGG | 0.490367 | 4:+84161073 | MsG0480023359.01.T01:CDS |
| AGGCTTCTTCAACCTTCTAG+TGG | 0.505384 | 4:-84160851 | None:intergenic |
| GAAATTGAGGTACTGGGTTG+TGG | 0.534342 | 4:-84160812 | None:intergenic |
| AAAACTTTGTTCCTGCAAAG+TGG | 0.534572 | 4:-84161015 | None:intergenic |
| AGCAGAGCACATAGATTGTA+GGG | 0.548771 | 4:+84160876 | MsG0480023359.01.T01:CDS |
| TTCAATGAAATTGAGGTACT+GGG | 0.560800 | 4:-84160818 | None:intergenic |
| GCTTCTTCAACCTTCTAGTG+GGG | 0.573302 | 4:-84160849 | None:intergenic |
| GTTTATCTGAGTTTGAGGAA+GGG | 0.622505 | 4:+84160902 | MsG0480023359.01.T01:CDS |
| ACTTTAGGTAACAAATGACA+AGG | 0.685765 | 4:-84160771 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATCAGGTAGAATTTGAT+GGG | + | Chr4:84161073-84161092 | MsG0480023359.01.T01:CDS | 25.0% |
| ! | CGATCAGTATATATGTAAAT+AGG | - | Chr4:84160744-84160763 | None:intergenic | 25.0% |
| !!! | TTGGATTTTTAGTTGACTTT+AGG | - | Chr4:84160789-84160808 | None:intergenic | 25.0% |
| ACTTTAGGTAACAAATGACA+AGG | - | Chr4:84160774-84160793 | None:intergenic | 30.0% | |
| ATAAGGATTGTTTGGACAAA+TGG | + | Chr4:84160962-84160981 | MsG0480023359.01.T01:CDS | 30.0% | |
| CAAAATCAGGTAGAATTTGA+TGG | + | Chr4:84161072-84161091 | MsG0480023359.01.T01:CDS | 30.0% | |
| CACATTTCATAAGGATTGTT+TGG | + | Chr4:84160954-84160973 | MsG0480023359.01.T01:CDS | 30.0% | |
| GTATTGTTTGCTTCAAAATC+AGG | + | Chr4:84161059-84161078 | MsG0480023359.01.T01:CDS | 30.0% | |
| TCTTCTCTTCAATGAAATTG+AGG | - | Chr4:84160828-84160847 | None:intergenic | 30.0% | |
| ! | TTCAATGAAATTGAGGTACT+GGG | - | Chr4:84160821-84160840 | None:intergenic | 30.0% |
| AAAACTTTGTTCCTGCAAAG+TGG | - | Chr4:84161018-84161037 | None:intergenic | 35.0% | |
| CTGTGAACACACATTTCATA+AGG | + | Chr4:84160945-84160964 | MsG0480023359.01.T01:CDS | 35.0% | |
| CTTCAATGAAATTGAGGTAC+TGG | - | Chr4:84160822-84160841 | None:intergenic | 35.0% | |
| GGTATGTTTATCTGAGTTTG+AGG | + | Chr4:84160897-84160916 | MsG0480023359.01.T01:CDS | 35.0% | |
| GTTTATCTGAGTTTGAGGAA+GGG | + | Chr4:84160902-84160921 | MsG0480023359.01.T01:CDS | 35.0% | |
| TGTTTATCTGAGTTTGAGGA+AGG | + | Chr4:84160901-84160920 | MsG0480023359.01.T01:CDS | 35.0% | |
| TTTATCTGAGTTTGAGGAAG+GGG | + | Chr4:84160903-84160922 | MsG0480023359.01.T01:CDS | 35.0% | |
| !! | TTGCAGGAACAAAGTTTTGT+CGG | + | Chr4:84161020-84161039 | MsG0480023359.01.T01:CDS | 35.0% |
| AGCAGAGCACATAGATTGTA+GGG | + | Chr4:84160876-84160895 | MsG0480023359.01.T01:CDS | 40.0% | |
| AAGAGAAGAACCCCACTAGA+AGG | + | Chr4:84160839-84160858 | MsG0480023359.01.T01:CDS | 45.0% | |
| AGGCTTCTTCAACCTTCTAG+TGG | - | Chr4:84160854-84160873 | None:intergenic | 45.0% | |
| CAGCAGAGCACATAGATTGT+AGG | + | Chr4:84160875-84160894 | MsG0480023359.01.T01:CDS | 45.0% | |
| GCTTCTTCAACCTTCTAGTG+GGG | - | Chr4:84160852-84160871 | None:intergenic | 45.0% | |
| GGCTTCTTCAACCTTCTAGT+GGG | - | Chr4:84160853-84160872 | None:intergenic | 45.0% | |
| TTGAGGAAGGGGACATTGTT+AGG | + | Chr4:84160914-84160933 | MsG0480023359.01.T01:CDS | 45.0% | |
| !! | AGGTACTGGGTTGTGGTAAT+TGG | - | Chr4:84160808-84160827 | None:intergenic | 45.0% |
| !! | GAAATTGAGGTACTGGGTTG+TGG | - | Chr4:84160815-84160834 | None:intergenic | 45.0% |
| CAATCTATGTGCTCTGCTGC+AGG | - | Chr4:84160874-84160893 | None:intergenic | 50.0% | |
| !! | GTGCTACTTGTCCACTTTGC+AGG | + | Chr4:84161004-84161023 | MsG0480023359.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 84160691 | 84161131 | 84160691 | ID=MsG0480023359.01;Name=MsG0480023359.01 |
| Chr4 | mRNA | 84160691 | 84161131 | 84160691 | ID=MsG0480023359.01.T01;Parent=MsG0480023359.01;Name=MsG0480023359.01.T01;_AED=0.04;_eAED=0.06;_QI=0|-1|0|1|-1|0|1|0|146 |
| Chr4 | exon | 84160691 | 84161131 | 84160691 | ID=MsG0480023359.01.T01:exon:1360;Parent=MsG0480023359.01.T01 |
| Chr4 | CDS | 84160691 | 84161131 | 84160691 | ID=MsG0480023359.01.T01:cds;Parent=MsG0480023359.01.T01 |
| Chr4 | mRNA | 84160981 | 84161091 | 84160981 | ID=MsG0480023359.01.T02;Parent=MsG0480023359.01;Name=MsG0480023359.01.T02;_AED=0.38;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|36 |
| Chr4 | exon | 84160981 | 84161091 | 84160981 | ID=MsG0480023359.01.T02:exon:1361;Parent=MsG0480023359.01.T02 |
| Chr4 | CDS | 84160981 | 84161091 | 84160981 | ID=MsG0480023359.01.T02:cds;Parent=MsG0480023359.01.T02 |
| Gene Sequence |
| Protein sequence |
