Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008361.01.T01 | KEH37521.1 | 86.301 | 73 | 9 | 1 | 1 | 73 | 1 | 72 | 2.78E-32 | 120 |
| MsG0280008361.01.T02 | KEH37521.1 | 86.364 | 44 | 6 | 0 | 1 | 44 | 29 | 72 | 1.02E-16 | 79.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008361.01.T01 | A0A072V7B3 | 86.301 | 73 | 9 | 1 | 1 | 73 | 1 | 72 | 1.33e-32 | 120 |
| MsG0280008361.01.T02 | A0A072V7B3 | 86.364 | 44 | 6 | 0 | 1 | 44 | 29 | 72 | 4.88e-17 | 79.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048856.01 | MsG0280008361.01 | 0.801961 | 7.031535e-49 | 3.402685e-46 |
| MsG0180001892.01 | MsG0280008361.01 | 0.805644 | 1.208374e-49 | 6.426942e-47 |
| MsG0180003361.01 | MsG0280008361.01 | 0.821194 | 4.597425e-53 | 3.715138e-50 |
| MsG0180003920.01 | MsG0280008361.01 | 0.819324 | 1.233177e-52 | 9.458852e-50 |
| MsG0180004099.01 | MsG0280008361.01 | 0.807810 | 4.213969e-50 | 2.369940e-47 |
| MsG0280007506.01 | MsG0280008361.01 | 0.800513 | 1.391202e-48 | 6.490167e-46 |
| MsG0280007955.01 | MsG0280008361.01 | 0.818971 | 1.483257e-52 | 1.126876e-49 |
| MsG0280008361.01 | MsG0280008362.01 | 0.889214 | 2.959236e-73 | 2.365648e-69 |
| MsG0280008361.01 | MsG0280009794.01 | 0.809921 | 1.490235e-50 | 8.862178e-48 |
| MsG0280008361.01 | MsG0280011014.01 | 0.819334 | 1.226756e-52 | 9.411820e-50 |
| MsG0280008361.01 | MsG0380011496.01 | 0.831636 | 1.492246e-55 | 1.628880e-52 |
| MsG0280008361.01 | MsG0380011501.01 | 0.830539 | 2.774880e-55 | 2.931995e-52 |
| MsG0280008361.01 | MsG0380013475.01 | 0.809108 | 2.226665e-50 | 1.295930e-47 |
| MsG0280008361.01 | MsG0380013476.01 | 0.806833 | 6.789734e-50 | 3.722818e-47 |
| MsG0280008361.01 | MsG0380017869.01 | 0.857347 | 1.736233e-62 | 4.272271e-59 |
| MsG0280008361.01 | MsG0480018909.01 | 0.824112 | 9.628026e-54 | 8.448999e-51 |
| MsG0280008361.01 | MsG0480019922.01 | 0.806736 | 7.116930e-50 | 3.892502e-47 |
| MsG0280008361.01 | MsG0480023359.01 | 0.801436 | 9.012276e-49 | 4.303711e-46 |
| MsG0280008361.01 | MsG0580024489.01 | 0.801658 | 8.116486e-49 | 3.897610e-46 |
| MsG0280008361.01 | MsG0580026610.01 | 0.807651 | 4.554543e-50 | 2.550834e-47 |
| MsG0280008361.01 | MsG0580027650.01 | 0.814170 | 1.765470e-51 | 1.176030e-48 |
| MsG0280008361.01 | MsG0580027925.01 | 0.819792 | 9.641269e-53 | 7.493414e-50 |
| MsG0280008361.01 | MsG0580028436.01 | 0.826221 | 3.055100e-54 | 2.847477e-51 |
| MsG0280008361.01 | MsG0580029441.01 | 0.802922 | 4.456585e-49 | 2.210152e-46 |
| MsG0280008361.01 | MsG0680030949.01 | 0.800700 | 1.274381e-48 | 5.973258e-46 |
| MsG0280008361.01 | MsG0780036752.01 | 0.800874 | 1.174352e-48 | 5.529122e-46 |
| MsG0280008361.01 | MsG0780038424.01 | 0.835733 | 1.412867e-56 | 1.743160e-53 |
| MsG0280008361.01 | MsG0780040846.01 | 0.808475 | 3.040540e-50 | 1.740270e-47 |
| MsG0280008361.01 | MsG0880042961.01 | 0.809794 | 1.586796e-50 | 9.405242e-48 |
| MsG0280008361.01 | MsG0880043034.01 | 0.813387 | 2.626337e-51 | 1.712804e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008361.01.T01 | MTR_2g040890 | 86.301 | 73 | 9 | 1 | 1 | 73 | 1 | 72 | 3.37e-36 | 120 |
| MsG0280008361.01.T01 | MTR_2g040740 | 69.841 | 63 | 18 | 1 | 1 | 63 | 1 | 62 | 1.32e-20 | 83.2 |
| MsG0280008361.01.T01 | MTR_2g040830 | 69.841 | 63 | 18 | 1 | 1 | 63 | 1 | 62 | 1.45e-20 | 82.8 |
| MsG0280008361.01.T01 | MTR_2g040850 | 58.462 | 65 | 26 | 1 | 1 | 65 | 1 | 64 | 2.66e-20 | 80.9 |
| MsG0280008361.01.T01 | MTR_2g040650 | 66.667 | 63 | 20 | 1 | 1 | 63 | 1 | 62 | 4.15e-18 | 76.3 |
| MsG0280008361.01.T01 | MTR_2g040690 | 68.750 | 48 | 14 | 1 | 16 | 63 | 18 | 64 | 3.97e-17 | 73.6 |
| MsG0280008361.01.T01 | MTR_2g040870 | 63.830 | 47 | 17 | 0 | 17 | 63 | 13 | 59 | 1.40e-16 | 69.7 |
| MsG0280008361.01.T01 | MTR_7g116850 | 42.857 | 63 | 36 | 0 | 1 | 63 | 24 | 86 | 5.10e-12 | 59.3 |
| MsG0280008361.01.T02 | MTR_2g040890 | 86.364 | 44 | 6 | 0 | 1 | 44 | 29 | 72 | 1.24e-20 | 79.3 |
| MsG0280008361.01.T02 | MTR_2g040870 | 64.706 | 34 | 12 | 0 | 1 | 34 | 26 | 59 | 2.37e-12 | 57.4 |
| MsG0280008361.01.T02 | MTR_2g040850 | 61.111 | 36 | 14 | 0 | 1 | 36 | 29 | 64 | 1.48e-11 | 56.2 |
| MsG0280008361.01.T02 | MTR_2g040740 | 64.706 | 34 | 12 | 0 | 1 | 34 | 29 | 62 | 1.87e-11 | 56.6 |
| MsG0280008361.01.T02 | MTR_2g040830 | 64.706 | 34 | 12 | 0 | 1 | 34 | 29 | 62 | 1.87e-11 | 56.6 |
| MsG0280008361.01.T02 | MTR_2g040690 | 64.706 | 34 | 12 | 0 | 1 | 34 | 31 | 64 | 6.01e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCAATATGGTTGTCTATT+GGG | 0.292929 | 2:+29978591 | MsG0280008361.01.T01:CDS |
| ATCATCAAATGGTGGCAATA+TGG | 0.331767 | 2:+29978579 | MsG0280008361.01.T01:CDS |
| GTGGCAATATGGTTGTCTAT+TGG | 0.373642 | 2:+29978590 | MsG0280008361.01.T01:CDS |
| ACCGCAATATGGTTTCAAAA+AGG | 0.375145 | 2:+29978503 | None:intergenic |
| CTTCTTCTTGAAAGATATTG+AGG | 0.406981 | 2:-29978687 | None:intergenic |
| GGCAATATGGTTGTCTATTG+GGG | 0.450971 | 2:+29978592 | MsG0280008361.01.T01:CDS |
| AATCCCAAGTTAAACTTCAT+AGG | 0.462233 | 2:+29978710 | MsG0280008361.01.T01:CDS |
| ATGACCTATGAAGTTTAACT+TGG | 0.472964 | 2:-29978714 | None:intergenic |
| TTGAAATCAACTTGTGACAC+TGG | 0.504021 | 2:+29978637 | MsG0280008361.01.T01:CDS |
| ATATTGCCACCATTTGATGA+TGG | 0.509465 | 2:-29978577 | None:intergenic |
| ACTATCAAACCATCATCAAA+TGG | 0.510231 | 2:+29978568 | MsG0280008361.01.T01:CDS |
| TGATGATGGTTTGATAGTGA+AGG | 0.576846 | 2:-29978563 | None:intergenic |
| TGACCTATGAAGTTTAACTT+GGG | 0.596903 | 2:-29978713 | None:intergenic |
| GATGATGGTTTGATAGTGAA+GGG | 0.600294 | 2:-29978562 | None:intergenic |
| ATCAAACCATCATCAAATGG+TGG | 0.640431 | 2:+29978571 | MsG0280008361.01.T01:CDS |
| ATGATGGTTTGATAGTGAAG+GGG | 0.664735 | 2:-29978561 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCCCAAGTTAAACTTCAT+AGG | + | Chr2:29978710-29978729 | MsG0280008361.01.T01:CDS | 30.0% | |
| ACTATCAAACCATCATCAAA+TGG | + | Chr2:29978568-29978587 | MsG0280008361.01.T01:CDS | 30.0% | |
| ATGACCTATGAAGTTTAACT+TGG | - | Chr2:29978717-29978736 | None:intergenic | 30.0% | |
| CTTCTTCTTGAAAGATATTG+AGG | - | Chr2:29978690-29978709 | None:intergenic | 30.0% | |
| ! | TGACCTATGAAGTTTAACTT+GGG | - | Chr2:29978716-29978735 | None:intergenic | 30.0% |
| ATATTGCCACCATTTGATGA+TGG | - | Chr2:29978580-29978599 | None:intergenic | 35.0% | |
| ATCAAACCATCATCAAATGG+TGG | + | Chr2:29978571-29978590 | MsG0280008361.01.T01:CDS | 35.0% | |
| ATCATCAAATGGTGGCAATA+TGG | + | Chr2:29978579-29978598 | MsG0280008361.01.T01:CDS | 35.0% | |
| TGGCAATATGGTTGTCTATT+GGG | + | Chr2:29978591-29978610 | MsG0280008361.01.T01:CDS | 35.0% | |
| TTGAAATCAACTTGTGACAC+TGG | + | Chr2:29978637-29978656 | MsG0280008361.01.T01:CDS | 35.0% | |
| !! | ATGATGGTTTGATAGTGAAG+GGG | - | Chr2:29978564-29978583 | None:intergenic | 35.0% |
| !! | GATGATGGTTTGATAGTGAA+GGG | - | Chr2:29978565-29978584 | None:intergenic | 35.0% |
| !! | TGATGATGGTTTGATAGTGA+AGG | - | Chr2:29978566-29978585 | None:intergenic | 35.0% |
| GGCAATATGGTTGTCTATTG+GGG | + | Chr2:29978592-29978611 | MsG0280008361.01.T01:CDS | 40.0% | |
| GTGGCAATATGGTTGTCTAT+TGG | + | Chr2:29978590-29978609 | MsG0280008361.01.T01:CDS | 40.0% | |
| !!! | GCAAGCATTGACTCTTTTCT+TGG | + | Chr2:29978525-29978544 | MsG0280008361.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 29978511 | 29978741 | 29978511 | ID=MsG0280008361.01;Name=MsG0280008361.01 |
| Chr2 | mRNA | 29978511 | 29978741 | 29978511 | ID=MsG0280008361.01.T01;Parent=MsG0280008361.01;Name=MsG0280008361.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|76 |
| Chr2 | exon | 29978511 | 29978741 | 29978511 | ID=MsG0280008361.01.T01:exon:6769;Parent=MsG0280008361.01.T01 |
| Chr2 | CDS | 29978511 | 29978741 | 29978511 | ID=MsG0280008361.01.T01:cds;Parent=MsG0280008361.01.T01 |
| Chr2 | mRNA | 29978598 | 29978741 | 29978598 | ID=MsG0280008361.01.T02;Parent=MsG0280008361.01;Name=MsG0280008361.01.T02;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|47 |
| Chr2 | exon | 29978598 | 29978741 | 29978598 | ID=MsG0280008361.01.T02:exon:6770;Parent=MsG0280008361.01.T02 |
| Chr2 | CDS | 29978598 | 29978741 | 29978598 | ID=MsG0280008361.01.T02:cds;Parent=MsG0280008361.01.T02 |
| Gene Sequence |
| Protein sequence |
