Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023607.01.T01 | PNX81251.1 | 60.976 | 164 | 29 | 3 | 1 | 129 | 1 | 164 | 3.64E-56 | 182 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023607.01.T01 | P31110 | 45.513 | 156 | 46 | 5 | 9 | 129 | 24 | 175 | 1.85E-32 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005687.01 | MsG0480023607.01 | 0.802992 | 4.311132e-49 | 2.141737e-46 |
| MsG0480018670.01 | MsG0480023607.01 | 0.809751 | 1.620745e-50 | 9.595838e-48 |
| MsG0480021830.01 | MsG0480023607.01 | 0.840698 | 7.433602e-58 | 1.067457e-54 |
| MsG0480023607.01 | MsG0580024340.01 | 0.803729 | 3.033687e-49 | 1.535953e-46 |
| MsG0480023607.01 | MsG0580028179.01 | 0.806643 | 7.446338e-50 | 4.062644e-47 |
| MsG0480023607.01 | MsG0680031996.01 | 0.800227 | 1.590984e-48 | 7.368442e-46 |
| MsG0480023607.01 | MsG0780036620.01 | 0.819972 | 8.768685e-53 | 6.849497e-50 |
| MsG0480023607.01 | MsG0780036846.01 | 0.807502 | 4.899961e-50 | 2.733785e-47 |
| MsG0480023607.01 | MsG0880042084.01 | 0.820775 | 5.739702e-53 | 4.584103e-50 |
| MsG0480023607.01 | MsG0880042416.01 | 0.805547 | 1.266388e-49 | 6.718498e-47 |
| MsG0480023607.01 | MsG0880042417.01 | 0.816131 | 6.477251e-52 | 4.551280e-49 |
| MsG0380014982.01 | MsG0480023607.01 | 0.803986 | 2.682442e-49 | 1.367192e-46 |
| MsG0380017683.01 | MsG0480023607.01 | 0.816829 | 4.519665e-52 | 3.236960e-49 |
| MsG0280006552.01 | MsG0480023607.01 | 0.830537 | 2.777639e-55 | 2.934778e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023607.01.T01 | MTR_8g096920 | 59.756 | 164 | 31 | 3 | 1 | 129 | 8 | 171 | 2.21e-59 | 181 |
| MsG0480023607.01.T01 | MTR_8g096910 | 57.317 | 164 | 35 | 3 | 1 | 129 | 11 | 174 | 3.39e-58 | 178 |
| MsG0480023607.01.T01 | MTR_8g096900 | 33.333 | 213 | 58 | 7 | 1 | 129 | 34 | 246 | 1.22e-23 | 92.4 |
| MsG0480023607.01.T01 | MTR_5g010635 | 33.498 | 203 | 53 | 6 | 9 | 129 | 21 | 223 | 5.45e-22 | 87.8 |
| MsG0480023607.01.T01 | MTR_5g010640 | 34.266 | 143 | 58 | 3 | 6 | 112 | 20 | 162 | 7.39e-18 | 77.0 |
| MsG0480023607.01.T01 | MTR_2g067990 | 29.592 | 196 | 60 | 7 | 11 | 129 | 26 | 220 | 2.49e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023607.01.T01 | AT4G11650 | 40.000 | 145 | 51 | 3 | 4 | 112 | 15 | 159 | 7.79e-27 | 100 |
| MsG0480023607.01.T01 | AT1G77700 | 34.211 | 114 | 36 | 2 | 8 | 84 | 57 | 168 | 4.75e-13 | 65.1 |
| MsG0480023607.01.T01 | AT1G77700 | 34.211 | 114 | 36 | 2 | 8 | 84 | 85 | 196 | 5.01e-13 | 65.1 |
| MsG0480023607.01.T01 | AT1G77700 | 34.211 | 114 | 36 | 2 | 8 | 84 | 57 | 168 | 6.41e-13 | 64.7 |
| MsG0480023607.01.T01 | AT1G75030 | 30.400 | 125 | 46 | 2 | 1 | 84 | 13 | 137 | 4.04e-11 | 58.9 |
Find 40 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTTTGCCCTTGAAATTGT+TGG | 0.340640 | 4:-87575116 | MsG0480023607.01.T01:CDS |
| GTCAGAACCAAGTGTGAATT+CGG | 0.365389 | 4:+87575319 | None:intergenic |
| ATTAATTTATCTGTGATTGA+TGG | 0.391582 | 4:-87575284 | MsG0480023607.01.T01:CDS |
| GGCAGTGATGGTTGTCAAAC+TGG | 0.398020 | 4:-87575398 | MsG0480023607.01.T01:CDS |
| GCTGCTACTCCCGTCAATAA+AGG | 0.413360 | 4:-87575522 | MsG0480023607.01.T01:intron |
| GTGAATTCGGCAAGGGTTAG+CGG | 0.425952 | 4:+87575332 | None:intergenic |
| TGAATTCGGCAAGGGTTAGC+GGG | 0.433684 | 4:+87575333 | None:intergenic |
| CCGGGGAGATGATAAGCATC+AGG | 0.438940 | 4:+87575185 | None:intergenic |
| ACCAATGTTCCTTCACTGTC+TGG | 0.440354 | 4:-87575548 | MsG0480023607.01.T01:CDS |
| GCCCTTGATTGCAGCTCATC+CGG | 0.442995 | 4:-87575362 | MsG0480023607.01.T01:CDS |
| TATAAAGGGTGAATCTATAT+AGG | 0.450019 | 4:+87575092 | None:intergenic |
| AACTGCTTACCTTTATTGAC+GGG | 0.462868 | 4:+87575513 | None:intergenic |
| TACCGGATGAGCTGCAATCA+AGG | 0.472730 | 4:+87575360 | None:intergenic |
| AGGGCAATTAGAATCCTTGC+AGG | 0.477226 | 4:+87575205 | None:intergenic |
| GAGGTTGAACTAAATTGCAT+TGG | 0.486855 | 4:+87575251 | None:intergenic |
| TGTATTCGTTCGTTTATCAC+CGG | 0.504853 | 4:+87575166 | None:intergenic |
| TCTGTGCTTGATATCTGCTC+AGG | 0.511370 | 4:-87575592 | MsG0480023607.01.T01:CDS |
| CAATTTAGTTCAACCTCTAG+TGG | 0.511442 | 4:-87575245 | MsG0480023607.01.T01:CDS |
| ATATAGGTCCAACAATTTCA+AGG | 0.518062 | 4:+87575108 | None:intergenic |
| ACCCTTGCCGAATTCACACT+TGG | 0.518284 | 4:-87575326 | MsG0480023607.01.T01:CDS |
| CAACTGCTTACCTTTATTGA+CGG | 0.524802 | 4:+87575512 | None:intergenic |
| TTTGACGGTTCAGGCAGTGA+TGG | 0.534748 | 4:-87575410 | MsG0480023607.01.T01:intron |
| TTATTGACGGGAGTAGCAGC+TGG | 0.547273 | 4:+87575525 | None:intergenic |
| TACTTTGTTGCATCCACTAG+AGG | 0.548592 | 4:+87575232 | None:intergenic |
| GAATTCGGCAAGGGTTAGCG+GGG | 0.553998 | 4:+87575334 | None:intergenic |
| TGTCAAACTGGTGATTGTGC+TGG | 0.557596 | 4:-87575386 | MsG0480023607.01.T01:CDS |
| GCCAGACAGTGAAGGAACAT+TGG | 0.558598 | 4:+87575547 | None:intergenic |
| CCTGATGCTTATCATCTCCC+CGG | 0.578309 | 4:-87575185 | MsG0480023607.01.T01:CDS |
| TATAGGTCCAACAATTTCAA+GGG | 0.578361 | 4:+87575109 | None:intergenic |
| GTATTCGTTCGTTTATCACC+GGG | 0.586920 | 4:+87575167 | None:intergenic |
| TGATATCTGCTCAGGCAGCA+AGG | 0.592249 | 4:-87575584 | MsG0480023607.01.T01:CDS |
| AGCAGCTGGCCAGACAGTGA+AGG | 0.595270 | 4:+87575539 | None:intergenic |
| CGAACGAATACATGCCCAGT+TGG | 0.595353 | 4:-87575155 | MsG0480023607.01.T01:CDS |
| AACCAAGTGTGAATTCGGCA+AGG | 0.607224 | 4:+87575324 | None:intergenic |
| ACCGGATGAGCTGCAATCAA+GGG | 0.609796 | 4:+87575361 | None:intergenic |
| CAACAAAGTACTAACCTGCA+AGG | 0.625089 | 4:-87575219 | MsG0480023607.01.T01:CDS |
| ACCAAGTGTGAATTCGGCAA+GGG | 0.626520 | 4:+87575325 | None:intergenic |
| CGGGGAGATGATAAGCATCA+GGG | 0.646159 | 4:+87575186 | None:intergenic |
| AATTCGGCAAGGGTTAGCGG+GGG | 0.672413 | 4:+87575335 | None:intergenic |
| TATTCGTTCGTTTATCACCG+GGG | 0.759174 | 4:+87575168 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATTTATCTGTGATTGA+TGG | - | Chr4:87575422-87575441 | MsG0480023607.01.T01:intron | 20.0% |
| ATATAGGTCCAACAATTTCA+AGG | + | Chr4:87575601-87575620 | None:intergenic | 30.0% | |
| TATAGGTCCAACAATTTCAA+GGG | + | Chr4:87575600-87575619 | None:intergenic | 30.0% | |
| !! | ATTTTATAGTTGGTACCAAC+TGG | + | Chr4:87575569-87575588 | None:intergenic | 30.0% |
| !! | TTTTATAGTTGGTACCAACT+GGG | + | Chr4:87575568-87575587 | None:intergenic | 30.0% |
| AACTGCTTACCTTTATTGAC+GGG | + | Chr4:87575196-87575215 | None:intergenic | 35.0% | |
| CAACTGCTTACCTTTATTGA+CGG | + | Chr4:87575197-87575216 | None:intergenic | 35.0% | |
| GAGGTTGAACTAAATTGCAT+TGG | + | Chr4:87575458-87575477 | None:intergenic | 35.0% | |
| TGTATTCGTTCGTTTATCAC+CGG | + | Chr4:87575543-87575562 | None:intergenic | 35.0% | |
| ! | CAATTTAGTTCAACCTCTAG+TGG | - | Chr4:87575461-87575480 | MsG0480023607.01.T01:intron | 35.0% |
| ! | GGCAAAGACGATTTTATAGT+TGG | + | Chr4:87575579-87575598 | None:intergenic | 35.0% |
| CGTCAAAATGGCAATTGGTT+CGG | + | Chr4:87575283-87575302 | None:intergenic | 40.0% | |
| CGTCAATAAAGGTAAGCAGT+TGG | - | Chr4:87575195-87575214 | MsG0480023607.01.T01:CDS | 40.0% | |
| GTATTCGTTCGTTTATCACC+GGG | + | Chr4:87575542-87575561 | None:intergenic | 40.0% | |
| GTCAGAACCAAGTGTGAATT+CGG | + | Chr4:87575390-87575409 | None:intergenic | 40.0% | |
| GTCTTTGCCCTTGAAATTGT+TGG | - | Chr4:87575590-87575609 | MsG0480023607.01.T01:CDS | 40.0% | |
| TACTTTGTTGCATCCACTAG+AGG | + | Chr4:87575477-87575496 | None:intergenic | 40.0% | |
| TATTCGTTCGTTTATCACCG+GGG | + | Chr4:87575541-87575560 | None:intergenic | 40.0% | |
| TGAACCGTCAAAATGGCAAT+TGG | + | Chr4:87575288-87575307 | None:intergenic | 40.0% | |
| ! | CAACAAAGTACTAACCTGCA+AGG | - | Chr4:87575487-87575506 | MsG0480023607.01.T01:intron | 40.0% |
| !!! | AATTGCCATTTTGACGGTTC+AGG | - | Chr4:87575287-87575306 | MsG0480023607.01.T01:CDS | 40.0% |
| !!! | CGAACCAATTGCCATTTTGA+CGG | - | Chr4:87575281-87575300 | MsG0480023607.01.T01:CDS | 40.0% |
| AACCAAGTGTGAATTCGGCA+AGG | + | Chr4:87575385-87575404 | None:intergenic | 45.0% | |
| AACCTCATCTGCCCGAATTT+GGG | - | Chr4:87575256-87575275 | MsG0480023607.01.T01:CDS | 45.0% | |
| ACCAAGTGTGAATTCGGCAA+GGG | + | Chr4:87575384-87575403 | None:intergenic | 45.0% | |
| ACCAATGTTCCTTCACTGTC+TGG | - | Chr4:87575158-87575177 | MsG0480023607.01.T01:CDS | 45.0% | |
| AGGGCAATTAGAATCCTTGC+AGG | + | Chr4:87575504-87575523 | None:intergenic | 45.0% | |
| TCTGTGCTTGATATCTGCTC+AGG | - | Chr4:87575114-87575133 | MsG0480023607.01.T01:CDS | 45.0% | |
| TGTCAAACTGGTGATTGTGC+TGG | - | Chr4:87575320-87575339 | MsG0480023607.01.T01:CDS | 45.0% | |
| !! | AATTGGTTCGGCCCCAAATT+CGG | + | Chr4:87575271-87575290 | None:intergenic | 45.0% |
| AAGGTAAGCAGTTGGACAGC+AGG | - | Chr4:87575203-87575222 | MsG0480023607.01.T01:CDS | 50.0% | |
| ACCCTTGCCGAATTCACACT+TGG | - | Chr4:87575380-87575399 | MsG0480023607.01.T01:CDS | 50.0% | |
| ACCGGATGAGCTGCAATCAA+GGG | + | Chr4:87575348-87575367 | None:intergenic | 50.0% | |
| ACCTCATCTGCCCGAATTTG+GGG | - | Chr4:87575257-87575276 | MsG0480023607.01.T01:CDS | 50.0% | |
| AGGTAAGCAGTTGGACAGCA+GGG | - | Chr4:87575204-87575223 | MsG0480023607.01.T01:CDS | 50.0% | |
| CACTGCCTGAACCGTCAAAA+TGG | + | Chr4:87575295-87575314 | None:intergenic | 50.0% | |
| CCTGATGCTTATCATCTCCC+CGG | - | Chr4:87575521-87575540 | MsG0480023607.01.T01:intron | 50.0% | |
| CGAACGAATACATGCCCAGT+TGG | - | Chr4:87575551-87575570 | MsG0480023607.01.T01:CDS | 50.0% | |
| CGGGGAGATGATAAGCATCA+GGG | + | Chr4:87575523-87575542 | None:intergenic | 50.0% | |
| GAACCTCATCTGCCCGAATT+TGG | - | Chr4:87575255-87575274 | MsG0480023607.01.T01:CDS | 50.0% | |
| GCCAGACAGTGAAGGAACAT+TGG | + | Chr4:87575162-87575181 | None:intergenic | 50.0% | |
| GCTGCTACTCCCGTCAATAA+AGG | - | Chr4:87575184-87575203 | MsG0480023607.01.T01:CDS | 50.0% | |
| GGCAGTGATGGTTGTCAAAC+TGG | - | Chr4:87575308-87575327 | MsG0480023607.01.T01:CDS | 50.0% | |
| GTGAATTCGGCAAGGGTTAG+CGG | + | Chr4:87575377-87575396 | None:intergenic | 50.0% | |
| TACCGGATGAGCTGCAATCA+AGG | + | Chr4:87575349-87575368 | None:intergenic | 50.0% | |
| TGATATCTGCTCAGGCAGCA+AGG | - | Chr4:87575122-87575141 | MsG0480023607.01.T01:CDS | 50.0% | |
| TTATTGACGGGAGTAGCAGC+TGG | + | Chr4:87575184-87575203 | None:intergenic | 50.0% | |
| TTTGACGGTTCAGGCAGTGA+TGG | - | Chr4:87575296-87575315 | MsG0480023607.01.T01:CDS | 50.0% | |
| ! | TGAATTCGGCAAGGGTTAGC+GGG | + | Chr4:87575376-87575395 | None:intergenic | 50.0% |
| !! | ATTGGTTCGGCCCCAAATTC+GGG | + | Chr4:87575270-87575289 | None:intergenic | 50.0% |
| CCGGGGAGATGATAAGCATC+AGG | + | Chr4:87575524-87575543 | None:intergenic | 55.0% | |
| GCCCTTGATTGCAGCTCATC+CGG | - | Chr4:87575344-87575363 | MsG0480023607.01.T01:CDS | 55.0% | |
| GGTAAGCAGTTGGACAGCAG+GGG | - | Chr4:87575205-87575224 | MsG0480023607.01.T01:CDS | 55.0% | |
| ! | AAGGGTTAGCGGGGGTTTAC+CGG | + | Chr4:87575366-87575385 | None:intergenic | 55.0% |
| ! | AATTCGGCAAGGGTTAGCGG+GGG | + | Chr4:87575374-87575393 | None:intergenic | 55.0% |
| ! | GAATTCGGCAAGGGTTAGCG+GGG | + | Chr4:87575375-87575394 | None:intergenic | 55.0% |
| AGCAGCTGGCCAGACAGTGA+AGG | + | Chr4:87575170-87575189 | None:intergenic | 60.0% | |
| AGCTGGGATGTCCAGGACCA+TGG | + | Chr4:87575235-87575254 | None:intergenic | 60.0% | |
| CGGGCAGATGAGGTTCAGCT+GGG | + | Chr4:87575251-87575270 | None:intergenic | 60.0% | |
| GAGGTTCAGCTGGGATGTCC+AGG | + | Chr4:87575242-87575261 | None:intergenic | 60.0% | |
| GCCCCAAATTCGGGCAGATG+AGG | + | Chr4:87575261-87575280 | None:intergenic | 60.0% | |
| GGGATGTCCAGGACCATGGT+TGG | + | Chr4:87575231-87575250 | None:intergenic | 60.0% | |
| TCGGGCAGATGAGGTTCAGC+TGG | + | Chr4:87575252-87575271 | None:intergenic | 60.0% | |
| TGGACAGCAGGGGCCAACCA+TGG | - | Chr4:87575215-87575234 | MsG0480023607.01.T01:CDS | 65.0% | |
| GCAGGGGCCAACCATGGTCC+TGG | - | Chr4:87575221-87575240 | MsG0480023607.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 87575106 | 87575622 | 87575106 | ID=MsG0480023607.01;Name=MsG0480023607.01 |
| Chr4 | mRNA | 87575106 | 87575622 | 87575106 | ID=MsG0480023607.01.T01;Parent=MsG0480023607.01;Name=MsG0480023607.01.T01;_AED=0.43;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|137 |
| Chr4 | exon | 87575523 | 87575622 | 87575523 | ID=MsG0480023607.01.T01:exon:1860;Parent=MsG0480023607.01.T01 |
| Chr4 | exon | 87575106 | 87575419 | 87575106 | ID=MsG0480023607.01.T01:exon:1859;Parent=MsG0480023607.01.T01 |
| Chr4 | CDS | 87575523 | 87575622 | 87575523 | ID=MsG0480023607.01.T01:cds;Parent=MsG0480023607.01.T01 |
| Chr4 | CDS | 87575106 | 87575419 | 87575106 | ID=MsG0480023607.01.T01:cds;Parent=MsG0480023607.01.T01 |
| Gene Sequence |
| Protein sequence |
