AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006552.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006552.01.T01 MTR_2g010600 90.184 163 15 1 1 163 1 162 1.96e-108 306
MsG0280006552.01.T01 MTR_4g050762 84.049 163 25 1 1 163 1 162 1.56e-94 271
MsG0280006552.01.T01 MTR_2g012370 84.049 163 25 1 1 163 1 162 1.56e-94 271
MsG0280006552.01.T01 MTR_2g010590 74.847 163 37 2 1 163 1 159 7.30e-89 256
MsG0280006552.01.T01 MTR_2g010650 67.647 170 43 7 1 163 1 165 2.37e-73 217
MsG0280006552.01.T01 MTR_2g010610 72.393 163 20 3 1 163 1 138 3.54e-72 213
MsG0280006552.01.T01 MTR_2g010630 69.872 156 39 5 13 163 13 165 9.70e-72 213
MsG0280006552.01.T01 MTR_2g010700 59.412 170 60 5 1 163 1 168 1.87e-65 197
MsG0280006552.01.T01 MTR_2g435490 54.717 159 68 2 6 163 9 164 5.16e-63 192
MsG0280006552.01.T01 MTR_2g010640 60.479 167 58 6 1 163 1 163 7.67e-61 186
MsG0280006552.01.T01 MTR_2g010670 57.738 168 62 6 1 163 1 164 1.21e-55 172
MsG0280006552.01.T01 MTR_2g010690 54.762 168 67 6 1 163 1 164 4.60e-53 166
MsG0280006552.01.T01 MTR_2g010580 50.331 151 70 3 13 163 19 164 5.94e-52 163
MsG0280006552.01.T01 MTR_4g092540 45.122 164 84 4 1 163 1 159 9.25e-45 145
MsG0280006552.01.T01 MTR_4g128750 50.735 136 63 2 29 163 41 173 2.25e-44 144
MsG0280006552.01.T01 MTR_4g128770 44.203 138 74 3 28 163 70 206 1.42e-38 130
MsG0280006552.01.T01 MTR_1g067290 42.029 138 74 4 28 162 73 207 6.40e-33 116
MsG0280006552.01.T01 MTR_8g078770 38.129 139 81 4 28 163 36 172 8.38e-26 97.1
MsG0280006552.01.T01 MTR_5g018770 31.844 179 103 4 4 163 7 185 1.17e-24 94.4
MsG0280006552.01.T01 MTR_4g092515 55.789 95 36 4 1 94 7 96 7.50e-23 88.2
MsG0280006552.01.T01 MTR_5g018755 38.462 91 51 4 78 163 13 103 1.16e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006552.01.T01 AT2G14580 64.242 165 51 4 1 163 3 161 5.29e-70 209
MsG0280006552.01.T01 AT4G33720 59.259 162 62 2 2 163 6 163 5.38e-66 199
MsG0280006552.01.T01 AT2G14610 58.788 165 60 4 1 163 3 161 4.84e-64 194
MsG0280006552.01.T01 AT5G26130 58.228 158 64 2 6 163 11 166 1.41e-59 182
MsG0280006552.01.T01 AT3G19690 53.333 165 71 3 1 163 1 161 5.13e-59 181
MsG0280006552.01.T01 AT4G33710 59.859 142 55 2 22 163 27 166 2.02e-55 172
MsG0280006552.01.T01 AT2G19990 54.962 131 56 1 33 163 49 176 4.33e-50 159
MsG0280006552.01.T01 AT1G50060 52.410 166 71 5 1 163 1 161 8.14e-50 157
MsG0280006552.01.T01 AT4G25790 49.640 139 66 2 26 163 75 210 9.77e-48 154
MsG0280006552.01.T01 AT3G09590 48.551 138 66 2 28 163 52 186 4.81e-46 149
MsG0280006552.01.T01 AT5G57625 49.265 136 65 2 29 163 75 207 7.64e-46 149
MsG0280006552.01.T01 AT1G50050 53.012 166 71 5 1 163 1 162 1.02e-45 147
MsG0280006552.01.T01 AT4G30320 45.033 151 78 3 14 163 15 161 6.42e-44 142
MsG0280006552.01.T01 AT4G25780 50.000 138 66 3 28 163 54 190 3.62e-43 142
MsG0280006552.01.T01 AT4G33730 48.428 159 68 5 14 163 19 172 3.97e-43 141
MsG0280006552.01.T01 AT1G50050 51.829 164 72 5 1 161 1 160 1.72e-42 141
MsG0280006552.01.T01 AT5G02730 47.482 139 68 2 27 163 58 193 2.21e-42 140
MsG0280006552.01.T01 AT1G01310 46.715 137 68 3 28 163 87 219 1.19e-39 134
MsG0280006552.01.T01 AT4G31470 44.604 139 72 3 26 163 51 185 2.37e-38 129
MsG0280006552.01.T01 AT4G07820 46.479 142 70 4 22 163 25 160 1.12e-37 127
MsG0280006552.01.T01 AT5G66590 42.336 137 77 1 29 163 49 185 5.18e-34 118
MsG0280006552.01.T01 AT2G19970 43.262 141 71 5 30 163 39 177 1.31e-29 107
MsG0280006552.01.T01 AT2G19980 37.427 171 85 8 3 163 7 165 6.67e-26 97.1

Find 38 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGGTTTATCCCTCATTA+TGG 0.279105 2:+3379511 MsG0280006552.01.T01:CDS
GGTGGCGGTGATAAACATTA+TGG 0.283064 2:+3379698 MsG0280006552.01.T01:CDS
CATAGTCTGCTTGTGAGTCT+TGG 0.335837 2:-3379550 None:intergenic
TATTAAGCATGCACTTAGTA+TGG 0.353177 2:-3379968 None:intergenic
GCCATTATACTCAGGTGGTT+TGG 0.358578 2:+3379840 MsG0280006552.01.T01:CDS
TCCAAACCACCTGAGTATAA+TGG 0.398812 2:-3379841 None:intergenic
ATAGTCTGCTTGTGAGTCTT+GGG 0.401577 2:-3379549 None:intergenic
TTGGTGTTGCAAATATTGTT+TGG 0.407510 2:+3379603 MsG0280006552.01.T01:CDS
GTTACTATTATAATCATAGT+TGG 0.429625 2:-3379792 None:intergenic
TGGTGTTGCAAATATTGTTT+GGG 0.451345 2:+3379604 MsG0280006552.01.T01:CDS
TTGACGTCTTGCATCGTTGT+GGG 0.459049 2:-3379579 None:intergenic
GTGGCGGTGATAAACATTAT+GGG 0.460708 2:+3379699 MsG0280006552.01.T01:CDS
ATTATTATCACATCTCACTT+TGG 0.463790 2:-3379888 None:intergenic
CGTTATTATAGTTGCCTGGT+GGG 0.465282 2:-3379940 None:intergenic
GAGAATCTAGCGTGGAGCTC+CGG 0.490150 2:+3379722 MsG0280006552.01.T01:CDS
GTGAGATGTGATAATAATAG+AGG 0.514467 2:+3379893 MsG0280006552.01.T01:CDS
ATCTGAGCCACTCATGTCGC+CGG 0.523273 2:-3379741 None:intergenic
CTTGACGTCTTGCATCGTTG+TGG 0.543312 2:-3379580 None:intergenic
GCTCAGATGCAGTGAAGTTG+TGG 0.543630 2:+3379756 MsG0280006552.01.T01:CDS
CTCAGATGCAGTGAAGTTGT+GGG 0.551141 2:+3379757 MsG0280006552.01.T01:CDS
TGGAGCTCCGGCGACATGAG+TGG 0.557633 2:+3379734 MsG0280006552.01.T01:CDS
GTGTAACATGAACCATAATG+AGG 0.566235 2:-3379523 None:intergenic
ATAATGTTTATCACCGCCAC+CGG 0.571825 2:-3379696 None:intergenic
TCGTTATTATAGTTGCCTGG+TGG 0.573306 2:-3379941 None:intergenic
AAACTATGCTAATCAACGCA+AGG 0.596763 2:+3379652 MsG0280006552.01.T01:CDS
TGGAGAAACTCAGTACGTGT+TGG 0.598633 2:+3379860 MsG0280006552.01.T01:CDS
ACATGCAATTACGACCCACC+AGG 0.608369 2:+3379926 MsG0280006552.01.T01:CDS
AACTATGCTAATCAACGCAA+GGG 0.616329 2:+3379653 MsG0280006552.01.T01:CDS
TGTAACATGAACCATAATGA+GGG 0.618907 2:-3379522 None:intergenic
AACGATGCAAGACGTCAAGT+TGG 0.620216 2:+3379584 MsG0280006552.01.T01:CDS
AAACTGATGCACTCCGGTGG+CGG 0.623369 2:+3379683 MsG0280006552.01.T01:CDS
GTGTGGCCATTATACTCAGG+TGG 0.627875 2:+3379835 MsG0280006552.01.T01:CDS
TGGCGGTGATAAACATTATG+GGG 0.629775 2:+3379700 MsG0280006552.01.T01:CDS
AGTGTGTGGCCATTATACTC+AGG 0.651003 2:+3379832 MsG0280006552.01.T01:CDS
GATTGTAAACTGATGCACTC+CGG 0.660976 2:+3379677 MsG0280006552.01.T01:CDS
TGTAAACTGATGCACTCCGG+TGG 0.705347 2:+3379680 MsG0280006552.01.T01:CDS
ATTATGGGGAGAATCTAGCG+TGG 0.720756 2:+3379714 MsG0280006552.01.T01:CDS
TGTGCTGCTAATAAAGTGTG+TGG 0.730769 2:+3379818 MsG0280006552.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTACTATTATAATCATAGT+TGG - Chr2:3379795-3379814 None:intergenic 20.0%
! ATTATTATCACATCTCACTT+TGG - Chr2:3379891-3379910 None:intergenic 25.0%
GTGAGATGTGATAATAATAG+AGG + Chr2:3379893-3379912 MsG0280006552.01.T01:CDS 30.0%
TGTAACATGAACCATAATGA+GGG - Chr2:3379525-3379544 None:intergenic 30.0%
! TTTTCGTTATTATAGTTGCC+TGG - Chr2:3379947-3379966 None:intergenic 30.0%
!! TGGTGTTGCAAATATTGTTT+GGG + Chr2:3379604-3379623 MsG0280006552.01.T01:CDS 30.0%
!! TTGGTGTTGCAAATATTGTT+TGG + Chr2:3379603-3379622 MsG0280006552.01.T01:CDS 30.0%
AAACTATGCTAATCAACGCA+AGG + Chr2:3379652-3379671 MsG0280006552.01.T01:CDS 35.0%
AACTATGCTAATCAACGCAA+GGG + Chr2:3379653-3379672 MsG0280006552.01.T01:CDS 35.0%
GTGTAACATGAACCATAATG+AGG - Chr2:3379526-3379545 None:intergenic 35.0%
! ATTTTCTCTATTGTGTGTGC+TGG + Chr2:3379493-3379512 MsG0280006552.01.T01:CDS 35.0%
! TTTTCTCTATTGTGTGTGCT+GGG + Chr2:3379494-3379513 MsG0280006552.01.T01:CDS 35.0%
ATAATGTTTATCACCGCCAC+CGG - Chr2:3379699-3379718 None:intergenic 40.0%
ATAGTCTGCTTGTGAGTCTT+GGG - Chr2:3379552-3379571 None:intergenic 40.0%
CGTTATTATAGTTGCCTGGT+GGG - Chr2:3379943-3379962 None:intergenic 40.0%
GATTGTAAACTGATGCACTC+CGG + Chr2:3379677-3379696 MsG0280006552.01.T01:CDS 40.0%
GTGGCGGTGATAAACATTAT+GGG + Chr2:3379699-3379718 MsG0280006552.01.T01:CDS 40.0%
TCCAAACCACCTGAGTATAA+TGG - Chr2:3379844-3379863 None:intergenic 40.0%
TCGTTATTATAGTTGCCTGG+TGG - Chr2:3379944-3379963 None:intergenic 40.0%
TGGCGGTGATAAACATTATG+GGG + Chr2:3379700-3379719 MsG0280006552.01.T01:CDS 40.0%
! TGTGCTGCTAATAAAGTGTG+TGG + Chr2:3379818-3379837 MsG0280006552.01.T01:CDS 40.0%
AACGATGCAAGACGTCAAGT+TGG + Chr2:3379584-3379603 MsG0280006552.01.T01:CDS 45.0%
AGTGTGTGGCCATTATACTC+AGG + Chr2:3379832-3379851 MsG0280006552.01.T01:CDS 45.0%
ATTATGGGGAGAATCTAGCG+TGG + Chr2:3379714-3379733 MsG0280006552.01.T01:CDS 45.0%
CATAGTCTGCTTGTGAGTCT+TGG - Chr2:3379553-3379572 None:intergenic 45.0%
CTCAGATGCAGTGAAGTTGT+GGG + Chr2:3379757-3379776 MsG0280006552.01.T01:CDS 45.0%
GCCATTATACTCAGGTGGTT+TGG + Chr2:3379840-3379859 MsG0280006552.01.T01:CDS 45.0%
GCTGGGTTTATCCCTCATTA+TGG + Chr2:3379511-3379530 MsG0280006552.01.T01:CDS 45.0%
TGGAGAAACTCAGTACGTGT+TGG + Chr2:3379860-3379879 MsG0280006552.01.T01:CDS 45.0%
TTGACGTCTTGCATCGTTGT+GGG - Chr2:3379582-3379601 None:intergenic 45.0%
! GGTGGCGGTGATAAACATTA+TGG + Chr2:3379698-3379717 MsG0280006552.01.T01:CDS 45.0%
ACATGCAATTACGACCCACC+AGG + Chr2:3379926-3379945 MsG0280006552.01.T01:CDS 50.0%
CTTGACGTCTTGCATCGTTG+TGG - Chr2:3379583-3379602 None:intergenic 50.0%
GCTCAGATGCAGTGAAGTTG+TGG + Chr2:3379756-3379775 MsG0280006552.01.T01:CDS 50.0%
GTGTGGCCATTATACTCAGG+TGG + Chr2:3379835-3379854 MsG0280006552.01.T01:CDS 50.0%
TGTAAACTGATGCACTCCGG+TGG + Chr2:3379680-3379699 MsG0280006552.01.T01:CDS 50.0%
AAACTGATGCACTCCGGTGG+CGG + Chr2:3379683-3379702 MsG0280006552.01.T01:CDS 55.0%
ATCTGAGCCACTCATGTCGC+CGG - Chr2:3379744-3379763 None:intergenic 55.0%
GAGAATCTAGCGTGGAGCTC+CGG + Chr2:3379722-3379741 MsG0280006552.01.T01:CDS 55.0%
TGGAGCTCCGGCGACATGAG+TGG + Chr2:3379734-3379753 MsG0280006552.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 3379485 3379976 3379485 ID=MsG0280006552.01;Name=MsG0280006552.01
Chr2 mRNA 3379485 3379976 3379485 ID=MsG0280006552.01.T01;Parent=MsG0280006552.01;Name=MsG0280006552.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|163
Chr2 exon 3379485 3379976 3379485 ID=MsG0280006552.01.T01:exon:1660;Parent=MsG0280006552.01.T01
Chr2 CDS 3379485 3379976 3379485 ID=MsG0280006552.01.T01:cds;Parent=MsG0280006552.01.T01
Gene Sequence

>MsG0280006552.01.T01

ATGAGTTCATTTTCTCTATTGTGTGTGCTGGGTTTATCCCTCATTATGGTTCATGTTACACATGCCCAAGACTCACAAGCAGACTATGTGAACGCCCACAACGATGCAAGACGTCAAGTTGGTGTTGCAAATATTGTTTGGGACAATACTGTTGCTGCTTTTGCACAAAACTATGCTAATCAACGCAAGGGTGATTGTAAACTGATGCACTCCGGTGGCGGTGATAAACATTATGGGGAGAATCTAGCGTGGAGCTCCGGCGACATGAGTGGCTCAGATGCAGTGAAGTTGTGGGTGAACGAGAAAGCCAACTATGATTATAATAGTAACACATGTGCTGCTAATAAAGTGTGTGGCCATTATACTCAGGTGGTTTGGAGAAACTCAGTACGTGTTGGATGTGCCAAAGTGAGATGTGATAATAATAGAGGAACATTCATTACATGCAATTACGACCCACCAGGCAACTATAATAACGAAAAGCCATACTAA

Protein sequence

>MsG0280006552.01.T01

MSSFSLLCVLGLSLIMVHVTHAQDSQADYVNAHNDARRQVGVANIVWDNTVAAFAQNYANQRKGDCKLMHSGGGDKHYGENLAWSSGDMSGSDAVKLWVNEKANYDYNSNTCAANKVCGHYTQVVWRNSVRVGCAKVRCDNNRGTFITCNYDPPGNYNNEKPY*