AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023921.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023921.01.T01 MTR_8g104890 43.195 169 9 3 1 82 1 169 1.05e-30 106
MsG0480023921.01.T01 MTR_5g017650 78.571 42 9 0 16 57 66 107 4.42e-18 74.3
MsG0480023921.01.T01 MTR_8g104870 72.093 43 12 0 15 57 62 104 8.51e-16 68.6
MsG0480023921.01.T01 MTR_8g104830 62.857 35 13 0 20 54 24 58 4.33e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023921.01.T01 AT1G14870 78.947 38 8 0 20 57 31 68 4.29e-16 68.6
MsG0480023921.01.T01 AT1G14870 78.947 38 8 0 20 57 31 68 5.62e-16 67.8
MsG0480023921.01.T01 AT1G68610 71.429 35 10 0 20 54 34 68 1.19e-13 62.4
MsG0480023921.01.T01 AT1G14880 71.429 35 10 0 20 54 30 64 9.83e-12 57.4
MsG0480023921.01.T01 AT3G18470 38.961 77 37 2 14 80 14 90 1.32e-11 56.6
MsG0480023921.01.T01 AT5G35525 60.526 38 15 0 20 57 31 68 1.90e-11 56.6

Find 23 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTGTCCATGCATCACCTT+TGG 0.421844 4:+91271678 MsG0480023921.01.T01:CDS
TTGCAGCTTGTGCTGTAAGC+GGG 0.457897 4:+91271822 MsG0480023921.01.T01:intron
TGCTGGAGCTGTTGGTGCCA+TGG 0.464678 4:-91272425 None:intergenic
GTGGCTGATTGTACGGTTGT+TGG 0.466033 4:-91271441 None:intergenic
TGTTCAACTGCTGGAGCTGT+TGG 0.477230 4:-91272433 None:intergenic
CGAGTCATGTGTTCAACTGC+TGG 0.481564 4:-91272442 None:intergenic
GAAATGTGGCACAAGGAACC+AGG 0.502641 4:+91272396 MsG0480023921.01.T01:CDS
GTGGCTGGTGGCTGATTGTA+CGG 0.517478 4:-91271448 None:intergenic
TTGGTGCCATGGCTACTCCC+TGG 0.530182 4:-91272414 None:intergenic
GTTCACCGGAAACCGGTGGC+TGG 0.554741 4:-91271463 None:intergenic
CACCGGAAACCGGTGGCTGG+TGG 0.556751 4:-91271460 None:intergenic
TACAATCAGCCACCAGCCAC+CGG 0.567328 4:+91271451 MsG0480023921.01.T01:CDS
ATTGCAGCTTGTGCTGTAAG+CGG 0.567979 4:+91271821 MsG0480023921.01.T01:intron
AAATTGGCTATAGGGTGGCA+TGG 0.569669 4:+91272374 MsG0480023921.01.T01:intron
TGCAGCTTGTGCTGTAAGCG+GGG 0.590429 4:+91271823 MsG0480023921.01.T01:intron
AATCTCAGCAATTCGTCCAA+AGG 0.597233 4:-91271694 None:intergenic
ATTCGTCCAAAGGTGATGCA+TGG 0.598061 4:-91271684 None:intergenic
AATTGCTGAGATTGTTGACA+AGG 0.603304 4:+91271704 MsG0480023921.01.T01:CDS
TATAGGGTGGCATGGAAATG+TGG 0.605383 4:+91272382 MsG0480023921.01.T01:intron
AAGGAACCAGGGAGTAGCCA+TGG 0.612064 4:+91272408 MsG0480023921.01.T01:CDS
TGGCATGGAAATGTGGCACA+AGG 0.665913 4:+91272389 MsG0480023921.01.T01:CDS
AAATGTGGCACAAGGAACCA+GGG 0.671495 4:+91272397 MsG0480023921.01.T01:CDS
ATTGCTGAGATTGTTGACAA+GGG 0.678367 4:+91271705 MsG0480023921.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTAAAATTTAGAAG+AGG - Chr4:91271615-91271634 None:intergenic 10.0%
!! TAGTTATATATCATTATTAA+TGG - Chr4:91271760-91271779 None:intergenic 10.0%
!!! ATTCTCTTAATTTTTTATTT+AGG + Chr4:91272154-91272173 MsG0480023921.01.T01:intron 10.0%
!!! TAAATTTCATTTCATTTTTT+AGG + Chr4:91271642-91271661 MsG0480023921.01.T01:intron 10.0%
!! CTTGTAAGATATTCATTAAT+TGG + Chr4:91271733-91271752 MsG0480023921.01.T01:intron 20.0%
!! TTAGAAGAGGAATTAATTAA+TGG - Chr4:91271602-91271621 None:intergenic 20.0%
!!! ATTTATTTGAAATTGGCTAT+AGG + Chr4:91272365-91272384 MsG0480023921.01.T01:intron 20.0%
!!! TTTATTTGAAATTGGCTATA+GGG + Chr4:91272366-91272385 MsG0480023921.01.T01:intron 20.0%
! AAGAGGAATTAATTAATGGA+CGG - Chr4:91271598-91271617 None:intergenic 25.0%
! GTTCTAATTACCACTTAATT+TGG + Chr4:91271571-91271590 MsG0480023921.01.T01:intron 25.0%
!! TATTGTTGTCTCATTTGTTA+CGG - Chr4:91271905-91271924 None:intergenic 25.0%
!!! ATACATTAATTTGTGTGTGA+CGG + Chr4:91271852-91271871 MsG0480023921.01.T01:intron 25.0%
! TAACATACAAGTTTTGCAGT+CGG - Chr4:91271550-91271569 None:intergenic 30.0%
! TCGACAATTCAATGTTTCTA+TGG + Chr4:91272114-91272133 MsG0480023921.01.T01:intron 30.0%
!! TACATTAATTTGTGTGTGAC+GGG + Chr4:91271853-91271872 MsG0480023921.01.T01:intron 30.0%
!!! TTTTAGGTTGCATAACGTTT+TGG + Chr4:91271658-91271677 MsG0480023921.01.T01:intron 30.0%
AATTGCTGAGATTGTTGACA+AGG + Chr4:91271704-91271723 MsG0480023921.01.T01:CDS 35.0%
ATTGCTGAGATTGTTGACAA+GGG + Chr4:91271705-91271724 MsG0480023921.01.T01:CDS 35.0%
TGAACTAAGCAATCACAACA+AGG - Chr4:91271944-91271963 None:intergenic 35.0%
! ATTTGAAATTGGCTATAGGG+TGG + Chr4:91272369-91272388 MsG0480023921.01.T01:intron 35.0%
! GGTTGTTGGAATTTTGAACT+TGG - Chr4:91271430-91271449 None:intergenic 35.0%
! TGTTGGAATTTTGAACTTGG+AGG - Chr4:91271427-91271446 None:intergenic 35.0%
AATCTCAGCAATTCGTCCAA+AGG - Chr4:91271697-91271716 None:intergenic 40.0%
AATGGACGGTCCAAATTAAG+TGG - Chr4:91271584-91271603 None:intergenic 40.0%
ATTTGTACCATTGTCGCATC+TGG + Chr4:91272299-91272318 MsG0480023921.01.T01:intron 40.0%
CAAGTTCCATGTCAAATCCA+CGG - Chr4:91272018-91272037 None:intergenic 40.0%
TGAAAACCGTGGATTTGACA+TGG + Chr4:91272009-91272028 MsG0480023921.01.T01:intron 40.0%
!! TAATTTGTGTGTGACGGGTT+TGG + Chr4:91271858-91271877 MsG0480023921.01.T01:intron 40.0%
AAATGTGGCACAAGGAACCA+GGG + Chr4:91272397-91272416 MsG0480023921.01.T01:CDS 45.0%
AAATTGGCTATAGGGTGGCA+TGG + Chr4:91272374-91272393 MsG0480023921.01.T01:intron 45.0%
AACCTAATGCCAACCAGGAA+TGG + Chr4:91271499-91271518 MsG0480023921.01.T01:intron 45.0%
AACTCATCCAGATGCGACAA+TGG - Chr4:91272309-91272328 None:intergenic 45.0%
ATTCGTCCAAAGGTGATGCA+TGG - Chr4:91271687-91271706 None:intergenic 45.0%
ATTGCAGCTTGTGCTGTAAG+CGG + Chr4:91271821-91271840 MsG0480023921.01.T01:intron 45.0%
CGTGGATTTGACATGGAACT+TGG + Chr4:91272016-91272035 MsG0480023921.01.T01:intron 45.0%
TATAGGGTGGCATGGAAATG+TGG + Chr4:91272382-91272401 MsG0480023921.01.T01:intron 45.0%
!!! TTAATTTATTTATTTGAAAT+TGG + Chr4:91272358-91272377 MsG0480023921.01.T01:intron 5.0%
CCAACAACCTAATGCCAACC+AGG + Chr4:91271494-91271513 MsG0480023921.01.T01:intron 50.0%
CGAGTCATGTGTTCAACTGC+TGG - Chr4:91272445-91272464 None:intergenic 50.0%
GAAATGTGGCACAAGGAACC+AGG + Chr4:91272396-91272415 MsG0480023921.01.T01:CDS 50.0%
GAAGCAGTCAAATAGACCCG+TGG - Chr4:91271526-91271545 None:intergenic 50.0%
GACCATTCCTGGTTGGCATT+AGG - Chr4:91271504-91271523 None:intergenic 50.0%
GTGGCTGATTGTACGGTTGT+TGG - Chr4:91271444-91271463 None:intergenic 50.0%
TATCGCGAGCTTGAAAACCG+TGG + Chr4:91271998-91272017 MsG0480023921.01.T01:intron 50.0%
TGGCATGGAAATGTGGCACA+AGG + Chr4:91272389-91272408 MsG0480023921.01.T01:CDS 50.0%
TGGTGTCCATGCATCACCTT+TGG + Chr4:91271678-91271697 MsG0480023921.01.T01:CDS 50.0%
TGTTCAACTGCTGGAGCTGT+TGG - Chr4:91272436-91272455 None:intergenic 50.0%
TTGCAGCTTGTGCTGTAAGC+GGG + Chr4:91271822-91271841 MsG0480023921.01.T01:intron 50.0%
! CCTGGTTGGCATTAGGTTGT+TGG - Chr4:91271497-91271516 None:intergenic 50.0%
! GGTTGGCATTAGGTTGTTGG+TGG - Chr4:91271494-91271513 None:intergenic 50.0%
AAGGAACCAGGGAGTAGCCA+TGG + Chr4:91272408-91272427 MsG0480023921.01.T01:CDS 55.0%
ATAGACCCGTGGACCATTCC+TGG - Chr4:91271515-91271534 None:intergenic 55.0%
GTGGCTGGTGGCTGATTGTA+CGG - Chr4:91271451-91271470 None:intergenic 55.0%
TACAATCAGCCACCAGCCAC+CGG + Chr4:91271451-91271470 MsG0480023921.01.T01:CDS 55.0%
TGCAGCTTGTGCTGTAAGCG+GGG + Chr4:91271823-91271842 MsG0480023921.01.T01:intron 55.0%
TGCCAACCAGGAATGGTCCA+CGG + Chr4:91271506-91271525 MsG0480023921.01.T01:intron 55.0%
!! TGTTGGTGGCCTACGTTCAC+CGG - Chr4:91271480-91271499 None:intergenic 55.0%
ACCCGTGGACCATTCCTGGT+TGG - Chr4:91271511-91271530 None:intergenic 60.0%
CTACGTTCACCGGAAACCGG+TGG - Chr4:91271470-91271489 None:intergenic 60.0%
GCCAACCAGGAATGGTCCAC+GGG + Chr4:91271507-91271526 MsG0480023921.01.T01:intron 60.0%
GGCCTACGTTCACCGGAAAC+CGG - Chr4:91271473-91271492 None:intergenic 60.0%
!! CACCGGTTTCCGGTGAACGT+AGG + Chr4:91271468-91271487 MsG0480023921.01.T01:intron 60.0%
!! TGCTGGAGCTGTTGGTGCCA+TGG - Chr4:91272428-91272447 None:intergenic 60.0%
!! TTGGTGCCATGGCTACTCCC+TGG - Chr4:91272417-91272436 None:intergenic 60.0%
GTTCACCGGAAACCGGTGGC+TGG - Chr4:91271466-91271485 None:intergenic 65.0%
!! AGCCACCAGCCACCGGTTTC+CGG + Chr4:91271458-91271477 MsG0480023921.01.T01:intron 65.0%
CACCGGAAACCGGTGGCTGG+TGG - Chr4:91271463-91271482 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 91271418 91272468 91271418 ID=MsG0480023921.01;Name=MsG0480023921.01
Chr4 mRNA 91271418 91272468 91271418 ID=MsG0480023921.01.T01;Parent=MsG0480023921.01;Name=MsG0480023921.01.T01;_AED=0.50;_eAED=0.55;_QI=0|0|0|1|0.66|0.75|4|0|82
Chr4 exon 91271418 91271472 91271418 ID=MsG0480023921.01.T01:exon:13251;Parent=MsG0480023921.01.T01
Chr4 exon 91271664 91271735 91271664 ID=MsG0480023921.01.T01:exon:13252;Parent=MsG0480023921.01.T01
Chr4 exon 91271828 91271867 91271828 ID=MsG0480023921.01.T01:exon:13253;Parent=MsG0480023921.01.T01
Chr4 exon 91272387 91272468 91272387 ID=MsG0480023921.01.T01:exon:13254;Parent=MsG0480023921.01.T01
Chr4 CDS 91271418 91271472 91271418 ID=MsG0480023921.01.T01:cds;Parent=MsG0480023921.01.T01
Chr4 CDS 91271664 91271735 91271664 ID=MsG0480023921.01.T01:cds;Parent=MsG0480023921.01.T01
Chr4 CDS 91271828 91271867 91271828 ID=MsG0480023921.01.T01:cds;Parent=MsG0480023921.01.T01
Chr4 CDS 91272387 91272468 91272387 ID=MsG0480023921.01.T01:cds;Parent=MsG0480023921.01.T01
Gene Sequence

>MsG0480023921.01.T01

ATGAATCCTCCAAGTTCAAAATTCCAACAACCGTACAATCAGCCACCAGCCACCGGTTGCATAACGTTTTGGTGTCCATGCATCACCTTTGGACGAATTGCTGAGATTGTTGACAAGGGATCAACTTCTTGTGCTGTAAGCGGGGCTCTATATACATTAATTTGTGTGGTGGCATGGAAATGTGGCACAAGGAACCAGGGAGTAGCCATGGCACCAACAGCTCCAGCAGTTGAACACATGACTCGTTGA

Protein sequence

>MsG0480023921.01.T01

MNPPSSKFQQPYNQPPATGCITFWCPCITFGRIAEIVDKGSTSCAVSGALYTLICVVAWKCGTRNQGVAMAPTAPAVEHMTR*