Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024068.01.T01 | XP_003610633.1 | 97.079 | 445 | 11 | 2 | 1 | 444 | 1 | 444 | 0 | 877 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000856.01 | MsG0580024068.01 | 0.800129 | 1.665610e-48 | 7.694949e-46 |
| MsG0580024068.01 | MsG0780040742.01 | 0.800787 | 1.223574e-48 | 5.747640e-46 |
| MsG0580024068.01 | MsG0880047594.01 | 0.809825 | 1.562816e-50 | 9.270373e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580026510.01 | MsG0580024068.01 | PPI |
| MsG0580026510.01 | MsG0580024068.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024068.01.T01 | MTR_5g005240 | 97.079 | 445 | 11 | 2 | 1 | 444 | 1 | 444 | 0.0 | 877 |
| MsG0580024068.01.T01 | MTR_1g106065 | 56.075 | 428 | 159 | 9 | 26 | 444 | 12 | 419 | 3.25e-149 | 431 |
| MsG0580024068.01.T01 | MTR_8g094880 | 44.662 | 459 | 170 | 7 | 17 | 442 | 15 | 422 | 6.47e-119 | 354 |
| MsG0580024068.01.T01 | MTR_8g094905 | 44.662 | 459 | 170 | 7 | 17 | 442 | 15 | 422 | 6.47e-119 | 354 |
| MsG0580024068.01.T01 | MTR_2g105270 | 45.898 | 451 | 201 | 7 | 8 | 443 | 24 | 446 | 3.81e-116 | 348 |
| MsG0580024068.01.T01 | MTR_5g045590 | 40.092 | 434 | 207 | 9 | 14 | 443 | 19 | 403 | 1.67e-95 | 293 |
| MsG0580024068.01.T01 | MTR_2g105270 | 48.883 | 358 | 144 | 6 | 23 | 365 | 39 | 372 | 1.24e-94 | 291 |
| MsG0580024068.01.T01 | MTR_1g040495 | 40.855 | 421 | 181 | 8 | 26 | 438 | 5 | 365 | 3.86e-94 | 288 |
| MsG0580024068.01.T01 | MTR_8g088670 | 40.323 | 434 | 187 | 10 | 23 | 443 | 46 | 420 | 4.09e-94 | 290 |
| MsG0580024068.01.T01 | MTR_2g105270 | 46.089 | 358 | 154 | 6 | 8 | 350 | 24 | 357 | 4.70e-85 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024068.01.T01 | AT3G19640 | 64.206 | 447 | 113 | 5 | 36 | 444 | 47 | 484 | 0.0 | 530 |
| MsG0580024068.01.T01 | AT1G80900 | 45.175 | 456 | 203 | 8 | 1 | 443 | 19 | 440 | 9.49e-115 | 344 |
| MsG0580024068.01.T01 | AT1G80900 | 45.175 | 456 | 203 | 8 | 1 | 443 | 19 | 440 | 9.49e-115 | 344 |
| MsG0580024068.01.T01 | AT1G16010 | 46.697 | 439 | 178 | 10 | 23 | 443 | 40 | 440 | 1.50e-114 | 343 |
| MsG0580024068.01.T01 | AT1G16010 | 46.697 | 439 | 178 | 10 | 23 | 443 | 40 | 440 | 1.50e-114 | 343 |
| MsG0580024068.01.T01 | AT1G16010 | 46.697 | 439 | 178 | 10 | 23 | 443 | 40 | 440 | 1.50e-114 | 343 |
| MsG0580024068.01.T01 | AT5G09690 | 43.823 | 429 | 181 | 6 | 19 | 443 | 23 | 395 | 6.71e-104 | 315 |
| MsG0580024068.01.T01 | AT5G09690 | 43.823 | 429 | 181 | 6 | 19 | 443 | 12 | 384 | 7.97e-104 | 314 |
| MsG0580024068.01.T01 | AT5G09690 | 46.373 | 386 | 150 | 5 | 19 | 400 | 23 | 355 | 2.56e-100 | 305 |
| MsG0580024068.01.T01 | AT3G58970 | 39.636 | 439 | 203 | 6 | 17 | 443 | 46 | 434 | 1.42e-99 | 305 |
Find 204 sgRNAs with CRISPR-Local
Find 281 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAAACATAATGTACAC+TGG | 0.529329 | 5:+433947 | None:intergenic |
| AAAATCAATTCTCACGAGGG+TGG | 0.644396 | 5:+433395 | None:intergenic |
| AAACAAACATAATGTACACT+GGG | 0.610252 | 5:+433948 | None:intergenic |
| AAAGTCAATAAGAATTTATA+AGG | 0.319840 | 5:+433020 | None:intergenic |
| AAATGGGTTGGGATCCTCTT+CGG | 0.445647 | 5:-433720 | MsG0580024068.01.T01:three_prime_UTR |
| AACAATGTTATAAATGGGTT+GGG | 0.394159 | 5:-433731 | MsG0580024068.01.T01:three_prime_UTR |
| AACAGCTGCCGCCGCCGTAC+CGG | 0.467572 | 5:-436477 | MsG0580024068.01.T01:CDS |
| AACGGTAGCGATGACCGGTG+GGG | 0.675836 | 5:-436500 | MsG0580024068.01.T01:CDS |
| AACGTCACCGGTAACGCCAA+CGG | 0.620696 | 5:-436518 | MsG0580024068.01.T01:CDS |
| AAGAATATTTAGGCAGATTA+AGG | 0.256973 | 5:-432762 | MsG0580024068.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCAGCTTATGAAGTAGT+AGG | - | Chr5:433753-433772 | MsG0580024068.01.T01:three_prime_UTR | 30.0% | |
| AATAGGAGAGGAAATGTTTA+AGG | + | Chr5:435469-435488 | None:intergenic | 30.0% | |
| ACCTACCTAAAAGTAATGTT+TGG | + | Chr5:436124-436143 | None:intergenic | 30.0% | |
| AGAATAGCAATTAAGAGAAG+TGG | + | Chr5:434960-434979 | None:intergenic | 30.0% | |
| AGCTGAACTACTGAATAATT+TGG | - | Chr5:436090-436109 | MsG0580024068.01.T01:CDS | 30.0% | |
| ATGTCACTAGATAAGTTCAA+GGG | - | Chr5:433630-433649 | MsG0580024068.01.T01:three_prime_UTR | 30.0% | |
| ATTCAGATACGTGATTTCAT+TGG | + | Chr5:435675-435694 | None:intergenic | 30.0% | |
| CAAGGAAATGTTAAATAGGA+AGG | + | Chr5:433318-433337 | None:intergenic | 30.0% | |
| CAATACTTGTAGATATACCT+TGG | + | Chr5:435265-435284 | None:intergenic | 30.0% | |
| CACAGTTTCAATTTGAGTAA+TGG | + | Chr5:436207-436226 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 432190 | 436623 | 432190 | ID=MsG0580024068.01;Name=MsG0580024068.01 |
| Chr5 | mRNA | 432190 | 436623 | 432190 | ID=MsG0580024068.01.T01;Parent=MsG0580024068.01;Name=MsG0580024068.01.T01;_AED=0.43;_eAED=0.43;_QI=74|1|1|1|1|1|2|2029|444 |
| Chr5 | exon | 432190 | 434506 | 432190 | ID=MsG0580024068.01.T01:exon:23076;Parent=MsG0580024068.01.T01 |
| Chr5 | exon | 435503 | 436623 | 435503 | ID=MsG0580024068.01.T01:exon:23075;Parent=MsG0580024068.01.T01 |
| Chr5 | five_prime_UTR | 436550 | 436623 | 436550 | ID=MsG0580024068.01.T01:five_prime_utr;Parent=MsG0580024068.01.T01 |
| Chr5 | CDS | 435503 | 436549 | 435503 | ID=MsG0580024068.01.T01:cds;Parent=MsG0580024068.01.T01 |
| Chr5 | CDS | 434219 | 434506 | 434219 | ID=MsG0580024068.01.T01:cds;Parent=MsG0580024068.01.T01 |
| Chr5 | three_prime_UTR | 432190 | 434218 | 432190 | ID=MsG0580024068.01.T01:three_prime_utr;Parent=MsG0580024068.01.T01 |
| Gene Sequence |
>MsG0580024068.01.T01 ATGCGAGGAAACGTCACCGGTAACGCCAACGGTAGCGATGACCGGTGGGGAACAGCTGCCGCCGCCGTACCGGCAATTCGGAAGAAAGGTACAAGCGTTCGTCAATGGCTGGTTGTTGACGGCACCGGCGAAGCACAGGTAATCGAGGCCGGAAAGCACGCCATCATGCGTCGTACCGGCCTCCCTGCACGTGACCTTCGAATCCTGGACCCTCTCCTCTCCTACCCTTCCACCGTTCTCGGAAGAGAAAGAGCAATCGTCATCAACTTAGAACACATCAAAGCAATTATCACCGCAAACGAAGTTCTTCTTCTCAATTCACGCGATCCTTCCGTTACTCCTTTCGTTCAAGAGCTTCAAGCCAGGATTCTTCGTCATCACGAAGCAACCACTACTCCTCGTCCTGATAATCAGGAAGATTCTCACGGCGGAATTAAGATTCTTCCGTTTGAATTCGTTGCGCTTGAAGCGTGTCTTGAAGCAGCTTGTAGTGTTTTGGAGAGTGAAGCAAAGACGCTGGAACAAGAGGCGCATCCTGCGCTTGATAAATTGACTTCGAAGATTAGTACTCTCAATTTGGAACGTGTTCGTCAGATTAAGAGCCGTTTGGTTGCGATTACTGGAAGGGTTCAAAAAGTGAGAGATGAATTGGAACACTTGCTTGATGATGATGAAGATATGGCGGAGATGTATCTTACTGAGAAGTCACTTCAACAGCAATTGGAACAAACCTCTGATGAAGACACTGTTGAAACTCCTGATCATGATGTTCATGATAATGGTGTTGATGTTAATCAAACTGGACCAGGTGCTGAGATATCGTTGGAAGCGGGTTTAGGAGGAGGAGAGGTGAATTATGAGGAGGATGAGGATAATCTGAATTCTAGAGAGCAAATGTTTGGTGCTTCGAATTTAAGGGATAGCCGCGGAACACGTGCAAGCACTACTTATAGTGTCACCACCACTAAGCTTGATGTTGAGGAGCTTGAGATGCTCTTGGAAGCTTACTTTGTTCAGATCGACGGCACTCTCAATAAGCTCTCAACGCTGAGGGAGTATGTTGATGACACAGAGGATTATATCAACATTATGCTTGATGACAAACAGAACCATCTTCTCCAAATGGGAGTCATGTTGACCACGGCAACTCTGGTGGTAAGTGCCTTCGTTGTTGTCGCTGGTATTTTTGGCATGAATATTAAAATTGAACTGTTTGATGATAAATTGTATGGGATGAGAGAGTTTATGTGGACTGTTGGTGGCGGCACTGCTGGGACCATATTCTTGTATGTGGTTGCTATTGCCTGGTGCAAGCACAAACGCTTGCTGGAGTGA |
| Protein sequence |
>MsG0580024068.01.T01 MRGNVTGNANGSDDRWGTAAAAVPAIRKKGTSVRQWLVVDGTGEAQVIEAGKHAIMRRTGLPARDLRILDPLLSYPSTVLGRERAIVINLEHIKAIITANEVLLLNSRDPSVTPFVQELQARILRHHEATTTPRPDNQEDSHGGIKILPFEFVALEACLEAACSVLESEAKTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEHLLDDDEDMAEMYLTEKSLQQQLEQTSDEDTVETPDHDVHDNGVDVNQTGPGAEISLEAGLGGGEVNYEEDEDNLNSREQMFGASNLRDSRGTRASTTYSVTTTKLDVEELEMLLEAYFVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIKIELFDDKLYGMREFMWTVGGGTAGTIFLYVVAIAWCKHKRLLE* |
