AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024068.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024068.01.T01 MTR_5g005240 97.079 445 11 2 1 444 1 444 0.0 877
MsG0580024068.01.T01 MTR_1g106065 56.075 428 159 9 26 444 12 419 3.25e-149 431
MsG0580024068.01.T01 MTR_8g094880 44.662 459 170 7 17 442 15 422 6.47e-119 354
MsG0580024068.01.T01 MTR_8g094905 44.662 459 170 7 17 442 15 422 6.47e-119 354
MsG0580024068.01.T01 MTR_2g105270 45.898 451 201 7 8 443 24 446 3.81e-116 348
MsG0580024068.01.T01 MTR_5g045590 40.092 434 207 9 14 443 19 403 1.67e-95 293
MsG0580024068.01.T01 MTR_2g105270 48.883 358 144 6 23 365 39 372 1.24e-94 291
MsG0580024068.01.T01 MTR_1g040495 40.855 421 181 8 26 438 5 365 3.86e-94 288
MsG0580024068.01.T01 MTR_8g088670 40.323 434 187 10 23 443 46 420 4.09e-94 290
MsG0580024068.01.T01 MTR_2g105270 46.089 358 154 6 8 350 24 357 4.70e-85 265
MsG0580024068.01.T01 MTR_7g096170 45.052 384 156 7 23 400 12 346 3.66e-83 260
MsG0580024068.01.T01 MTR_7g096170 42.755 421 181 9 23 435 12 380 5.89e-83 260
MsG0580024068.01.T01 MTR_1g076210 39.588 437 196 11 21 443 6 388 2.41e-81 256
MsG0580024068.01.T01 MTR_1g093280 39.588 437 196 11 21 443 6 388 2.41e-81 256
MsG0580024068.01.T01 MTR_7g096170 44.253 348 137 7 23 363 12 309 2.58e-67 218
MsG0580024068.01.T01 MTR_8g088670 38.973 331 154 6 23 350 46 331 8.60e-64 209
MsG0580024068.01.T01 MTR_8g088670 38.576 337 141 7 23 347 46 328 1.33e-63 209
MsG0580024068.01.T01 MTR_7g096170 38.922 334 145 8 109 435 2 283 1.25e-47 165
MsG0580024068.01.T01 MTR_1g040475 44.737 190 98 3 36 223 20 204 1.47e-42 150
MsG0580024068.01.T01 MTR_2g100150 56.522 161 35 7 34 190 15 144 7.76e-40 140
MsG0580024068.01.T01 MTR_1g093370 50.420 119 56 1 325 443 51 166 7.36e-30 114
MsG0580024068.01.T01 MTR_1g076220 50.420 119 56 1 325 443 113 228 2.03e-29 115
MsG0580024068.01.T01 MTR_1g076220 56.000 50 22 0 187 236 53 102 2.06e-11 64.3
MsG0580024068.01.T01 MTR_1g055050 54.762 126 38 4 118 243 35 141 1.98e-28 113
MsG0580024068.01.T01 MTR_2g059110 55.000 80 36 0 26 105 8 87 6.02e-23 93.2
MsG0580024068.01.T01 MTR_2g059120 62.121 66 25 0 40 105 20 85 3.75e-20 85.5
MsG0580024068.01.T01 MTR_1g040455 38.298 94 55 1 350 443 7 97 6.60e-15 70.9
MsG0580024068.01.T01 MTR_2g084565 23.171 410 221 13 40 443 123 444 6.92e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024068.01.T01 AT3G19640 64.206 447 113 5 36 444 47 484 0.0 530
MsG0580024068.01.T01 AT1G80900 45.175 456 203 8 1 443 19 440 9.49e-115 344
MsG0580024068.01.T01 AT1G80900 45.175 456 203 8 1 443 19 440 9.49e-115 344
MsG0580024068.01.T01 AT1G16010 46.697 439 178 10 23 443 40 440 1.50e-114 343
MsG0580024068.01.T01 AT1G16010 46.697 439 178 10 23 443 40 440 1.50e-114 343
MsG0580024068.01.T01 AT1G16010 46.697 439 178 10 23 443 40 440 1.50e-114 343
MsG0580024068.01.T01 AT5G09690 43.823 429 181 6 19 443 23 395 6.71e-104 315
MsG0580024068.01.T01 AT5G09690 43.823 429 181 6 19 443 12 384 7.97e-104 314
MsG0580024068.01.T01 AT5G09690 46.373 386 150 5 19 400 23 355 2.56e-100 305
MsG0580024068.01.T01 AT3G58970 39.636 439 203 6 17 443 46 434 1.42e-99 305
MsG0580024068.01.T01 AT2G03620 41.289 419 207 8 25 443 40 419 4.66e-95 293
MsG0580024068.01.T01 AT2G03620 41.289 419 207 8 25 443 40 419 4.66e-95 293
MsG0580024068.01.T01 AT5G09690 45.833 360 142 4 19 376 12 320 1.06e-89 276
MsG0580024068.01.T01 AT5G09690 45.856 362 139 5 19 376 23 331 1.08e-89 276
MsG0580024068.01.T01 AT5G09690 45.856 362 139 5 19 376 23 331 1.08e-89 276
MsG0580024068.01.T01 AT5G09690 46.089 358 140 4 19 374 12 318 1.99e-89 275
MsG0580024068.01.T01 AT5G09690 46.089 358 140 4 19 374 23 329 2.74e-89 275
MsG0580024068.01.T01 AT5G09690 46.089 358 140 4 19 374 12 318 3.15e-89 275
MsG0580024068.01.T01 AT4G28580 33.796 432 203 11 27 444 46 408 3.84e-67 220
MsG0580024068.01.T01 AT5G09690 53.881 219 93 2 19 235 23 235 9.21e-67 216
MsG0580024068.01.T01 AT5G09690 53.881 219 93 2 19 235 23 235 9.21e-67 216
MsG0580024068.01.T01 AT5G09690 53.881 219 93 2 19 235 23 235 9.21e-67 216
MsG0580024068.01.T01 AT5G09690 53.881 219 93 2 19 235 12 224 1.06e-66 215
MsG0580024068.01.T01 AT5G09690 36.300 427 201 8 19 443 12 369 2.68e-63 209
MsG0580024068.01.T01 AT5G09720 39.185 319 140 7 23 337 8 276 9.12e-58 192
MsG0580024068.01.T01 AT5G09720 39.185 319 140 7 23 337 8 276 9.12e-58 192
MsG0580024068.01.T01 AT5G64560 47.794 136 67 2 308 443 261 392 7.75e-32 125
MsG0580024068.01.T01 AT5G64560 50.806 124 44 3 308 431 261 367 3.06e-29 118
MsG0580024068.01.T01 AT5G22830 29.016 193 118 4 39 227 135 312 2.71e-11 65.9
MsG0580024068.01.T01 AT5G22830 29.016 193 118 4 39 227 135 312 2.71e-11 65.9

Find 204 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGAACAATGTTATAAA+TGG 0.138594 5:-433737 MsG0580024068.01.T01:three_prime_UTR
TGTTTGGTGCTTCGAATTTA+AGG 0.165870 5:-435632 MsG0580024068.01.T01:CDS
AAGCTTCTTTAAACAATATT+TGG 0.189663 5:-433887 MsG0580024068.01.T01:three_prime_UTR
ACGCATGATGGCGTGCTTTC+CGG 0.208340 5:+436379 None:intergenic
AGCTGCCCCTGGCAGTTAAA+AGG 0.210678 5:+433217 None:intergenic
GAAGATTAGTACTCTCAATT+TGG 0.225739 5:-435970 MsG0580024068.01.T01:CDS
TTAACACATAATTATGAATT+AGG 0.232802 5:+432184 None:intergenic
ACTTCTCTTAATTGCTATTC+TGG 0.235540 5:-433832 MsG0580024068.01.T01:three_prime_UTR
ACTGTTGGTGGCGGCACTGC+TGG 0.237530 5:-434280 MsG0580024068.01.T01:CDS
AGCCGTTTGGTTGCGATTAC+TGG 0.237629 5:-435927 MsG0580024068.01.T01:CDS
AAGAATATTTAGGCAGATTA+AGG 0.256973 5:-432762 MsG0580024068.01.T01:three_prime_UTR
CTCTAACATGATGTTACTTT+TGG 0.264291 5:-432481 MsG0580024068.01.T01:three_prime_UTR
AATAGGAGAGGAAATGTTTA+AGG 0.264805 5:+433325 None:intergenic
CTAACTGTCACATGGTATTA+TGG 0.270739 5:-433071 MsG0580024068.01.T01:three_prime_UTR
CTTAGTGGCAAAGAATATTT+AGG 0.277601 5:-432772 MsG0580024068.01.T01:three_prime_UTR
TGAAGCAGCTTGTAGTGTTT+TGG 0.284919 5:-436051 MsG0580024068.01.T01:CDS
GCTAGAGTCCATTGTGTTTA+GGG 0.289725 5:-432420 MsG0580024068.01.T01:three_prime_UTR
TGCTAGAGTCCATTGTGTTT+AGG 0.297621 5:-432421 MsG0580024068.01.T01:three_prime_UTR
ATCGCTACCGTTGGCGTTAC+CGG 0.300032 5:+436511 None:intergenic
TAGGGGATCAACGTAACTTT+AGG 0.300648 5:-432402 MsG0580024068.01.T01:three_prime_UTR
AATGTGTCTGACTCTGTTTA+TGG 0.318761 5:-433786 MsG0580024068.01.T01:three_prime_UTR
AAAGTCAATAAGAATTTATA+AGG 0.319840 5:+433020 None:intergenic
ATATCGTTGGAAGCGGGTTT+AGG 0.335773 5:-435711 MsG0580024068.01.T01:CDS
GTGCAAGCACAAACGCTTGC+TGG 0.343936 5:-434224 MsG0580024068.01.T01:CDS
GTACCTTTCTTCCGAATTGC+CGG 0.346470 5:+436458 None:intergenic
TCCTACCCTTCCACCGTTCT+CGG 0.346952 5:-436308 MsG0580024068.01.T01:CDS
AGCTGAACTACTGAATAATT+TGG 0.349237 5:-432701 MsG0580024068.01.T01:three_prime_UTR
CCCATTTATAACATTGTTCA+AGG 0.353636 5:+433736 None:intergenic
TTCATTTCTGCTGTATTCTC+TGG 0.356117 5:-433177 MsG0580024068.01.T01:three_prime_UTR
CTATTCTGGTTTATAAACTT+TGG 0.362420 5:-433818 MsG0580024068.01.T01:three_prime_UTR
CTGTTGGTGGCGGCACTGCT+GGG 0.366775 5:-434279 MsG0580024068.01.T01:CDS
TTCGTATAAAATTTAAGTTC+AGG 0.370414 5:-432236 MsG0580024068.01.T01:three_prime_UTR
TGATACAGTGTACTAGTAAT+CGG 0.375155 5:+432806 None:intergenic
AATTCTTATTGACTTTGAAT+TGG 0.376478 5:-433013 MsG0580024068.01.T01:three_prime_UTR
TGAGAATCTTCCTGATTATC+AGG 0.379240 5:+436125 None:intergenic
TACTCCTCGTCCTGATAATC+AGG 0.381579 5:-436135 MsG0580024068.01.T01:CDS
CACAGTTTCAATTTGAGTAA+TGG 0.386934 5:+432587 None:intergenic
GTTTGGTGCTTCGAATTTAA+GGG 0.389449 5:-435631 MsG0580024068.01.T01:CDS
TATTCAATGATCTCCATGTT+TGG 0.389712 5:-433251 MsG0580024068.01.T01:three_prime_UTR
ATCAAAATGACCTGTCTTAG+TGG 0.390473 5:-432787 MsG0580024068.01.T01:three_prime_UTR
AACAATGTTATAAATGGGTT+GGG 0.394159 5:-433731 MsG0580024068.01.T01:three_prime_UTR
CATAGTCTTCCATGAGTTTG+AGG 0.396026 5:-433553 MsG0580024068.01.T01:three_prime_UTR
GGTGTTGATGTTAATCAAAC+TGG 0.400482 5:-435747 MsG0580024068.01.T01:CDS
CTGTTTGATGATAAATTGTA+TGG 0.401637 5:-434322 MsG0580024068.01.T01:CDS
TCTTACTCTTTCCATGACTT+TGG 0.405809 5:-432451 MsG0580024068.01.T01:three_prime_UTR
CAAGCGTTTGTGCTTGCACC+AGG 0.405920 5:+434228 None:intergenic
ATTCAGATACGTGATTTCAT+TGG 0.407014 5:+433119 None:intergenic
CTGGTATAGGTCTTAAAGTC+AGG 0.409153 5:+433489 None:intergenic
AGAAAAGGAAACACGAAGAA+TGG 0.412211 5:+432926 None:intergenic
TGCTCTTTCTCTTCCGAGAA+CGG 0.416716 5:+436295 None:intergenic
ACCTACCTAAAAGTAATGTT+TGG 0.419067 5:+432670 None:intergenic
TGCACGTGACCTTCGAATCC+TGG 0.419970 5:-436342 MsG0580024068.01.T01:CDS
GTTTATAAACTTTGGAAGCC+TGG 0.421298 5:-433810 MsG0580024068.01.T01:three_prime_UTR
AATTGTTTACTGTACAATAG+TGG 0.427454 5:+432343 None:intergenic
ACACGAAGAATGGGATTGAT+AGG 0.429328 5:+432936 None:intergenic
GATCATGATGTTCATGATAA+TGG 0.430175 5:-435768 MsG0580024068.01.T01:CDS
AAGTACACACGCACTTACTT+TGG 0.435537 5:+432309 None:intergenic
GAAGTCACTTCAACAGCAAT+TGG 0.436400 5:-435826 MsG0580024068.01.T01:CDS
AGCAACCACATACAAGAATA+TGG 0.438257 5:+434255 None:intergenic
ACAGAACCATCTTCTCCAAA+TGG 0.439397 5:-434428 MsG0580024068.01.T01:CDS
CCGCCGCCGTACCGGCAATT+CGG 0.439847 5:-436469 MsG0580024068.01.T01:CDS
CTCGATTACCTGTGCTTCGC+CGG 0.441216 5:+436403 None:intergenic
CTCCTCAACATCAAGCTTAG+TGG 0.445396 5:+435566 None:intergenic
AAATGGGTTGGGATCCTCTT+CGG 0.445647 5:-433720 MsG0580024068.01.T01:three_prime_UTR
TGTATGTGGTTGCTATTGCC+TGG 0.445914 5:-434246 MsG0580024068.01.T01:CDS
AGTGCCTTCGTTGTTGTCGC+TGG 0.447201 5:-434373 MsG0580024068.01.T01:CDS
CCTTGAACAATGTTATAAAT+GGG 0.448877 5:-433736 MsG0580024068.01.T01:three_prime_UTR
AATTCTAGAGAGCAAATGTT+TGG 0.448954 5:-435648 MsG0580024068.01.T01:CDS
ATATTCTTTGCCACTAAGAC+AGG 0.449381 5:+432777 None:intergenic
TGACTCCCATTTGGAGAAGA+TGG 0.450914 5:+434422 None:intergenic
GAAAATATATTAGAAAGAAA+AGG 0.457107 5:+432911 None:intergenic
TAAGTGCGTGTGTACTTTGA+AGG 0.458167 5:-432303 MsG0580024068.01.T01:three_prime_UTR
GAACAATGTTATAAATGGGT+TGG 0.462701 5:-433732 MsG0580024068.01.T01:three_prime_UTR
GAAAAGGAAACACGAAGAAT+GGG 0.464278 5:+432927 None:intergenic
GCTTGATGATGATGAAGATA+TGG 0.464583 5:-435868 MsG0580024068.01.T01:CDS
AACAGCTGCCGCCGCCGTAC+CGG 0.467572 5:-436477 MsG0580024068.01.T01:CDS
TGAGGAGCTTGAGATGCTCT+TGG 0.467583 5:-435550 MsG0580024068.01.T01:CDS
AGGAGGAGAGGTGAATTATG+AGG 0.467721 5:-435688 MsG0580024068.01.T01:CDS
GGTGCATTATTTATCTGACA+TGG 0.469649 5:-432520 MsG0580024068.01.T01:three_prime_UTR
CAAACGCTTGCTGGAGTGAA+TGG 0.472005 5:-434215 MsG0580024068.01.T01:exon
CATGTTGACCACGGCAACTC+TGG 0.474836 5:-434401 MsG0580024068.01.T01:CDS
ATTCTCATTTGAGCTGCCCC+TGG 0.474985 5:+433206 None:intergenic
GAGAAGAAGAACTTCGTTTG+CGG 0.477402 5:+436235 None:intergenic
GGTGAATTATGAGGAGGATG+AGG 0.479462 5:-435679 MsG0580024068.01.T01:CDS
TTCGTGATGACGAAGAATCC+TGG 0.480391 5:+436166 None:intergenic
GGTAGGAGAGGAGAGGGTCC+AGG 0.480489 5:+436324 None:intergenic
CCGAATTGCCGGTACGGCGG+CGG 0.484334 5:+436469 None:intergenic
CGTCAATGGCTGGTTGTTGA+CGG 0.484415 5:-436428 MsG0580024068.01.T01:CDS
CACGCCATCATGCGTCGTAC+CGG 0.485557 5:-436371 MsG0580024068.01.T01:CDS
TGGTCAACATGACTCCCATT+TGG 0.493890 5:+434413 None:intergenic
TCAAGGATATTCTGATACCA+TGG 0.497548 5:+433753 None:intergenic
TTTATGGCTTTATATGCCCA+TGG 0.497857 5:-433770 MsG0580024068.01.T01:three_prime_UTR
GCTGCCCCTGGCAGTTAAAA+GGG 0.499047 5:+433218 None:intergenic
GTTTGTACTAGAGTCACAAC+AGG 0.505271 5:-433421 MsG0580024068.01.T01:three_prime_UTR
GACAGAGATGTTCAATCTGA+TGG 0.505755 5:+433595 None:intergenic
AAGGTACAAGCGTTCGTCAA+TGG 0.506236 5:-436442 MsG0580024068.01.T01:CDS
AGGATTCGAAGGTCACGTGC+AGG 0.509325 5:+436344 None:intergenic
AGCGGGTTTAGGAGGAGGAG+AGG 0.510440 5:-435700 MsG0580024068.01.T01:CDS
AGAATAGCAATTAAGAGAAG+TGG 0.510827 5:+433834 None:intergenic
TGGGACCATATTCTTGTATG+TGG 0.512820 5:-434260 MsG0580024068.01.T01:CDS
TAACATCATGTTAGAGCTGG+TGG 0.514940 5:+432488 None:intergenic
TTATCATGAACATCATGATC+AGG 0.515588 5:+435771 None:intergenic
CAGAACCATCTTCTCCAAAT+GGG 0.517721 5:-434427 MsG0580024068.01.T01:CDS
AAGGTCACGTGCAGGGAGGC+CGG 0.519707 5:+436352 None:intergenic
TTGGAAGCGGGTTTAGGAGG+AGG 0.523144 5:-435705 MsG0580024068.01.T01:CDS
TCGTCAGATTAAGAGCCGTT+TGG 0.523284 5:-435940 MsG0580024068.01.T01:CDS
GAGTTTATGTGGACTGTTGG+TGG 0.523529 5:-434292 MsG0580024068.01.T01:CDS
TCGTTCAAGAGCTTCAAGCC+AGG 0.526920 5:-436184 MsG0580024068.01.T01:CDS
TACCTTGGTCCTCAAACTCA+TGG 0.527520 5:+433544 None:intergenic
TACAAGCGTTCGTCAATGGC+TGG 0.527925 5:-436438 MsG0580024068.01.T01:CDS
AAAACAAACATAATGTACAC+TGG 0.529329 5:+433947 None:intergenic
TGCTGAGATATCGTTGGAAG+CGG 0.529462 5:-435718 MsG0580024068.01.T01:CDS
GTTTGGTTGCGATTACTGGA+AGG 0.530868 5:-435923 MsG0580024068.01.T01:CDS
TTTGGTTGCGATTACTGGAA+GGG 0.531763 5:-435922 MsG0580024068.01.T01:CDS
CTAGAGTCCATTGTGTTTAG+GGG 0.532971 5:-432419 MsG0580024068.01.T01:three_prime_UTR
TGGCTGGTTGTTGACGGCAC+CGG 0.538585 5:-436422 MsG0580024068.01.T01:CDS
GATAGGTCTATGAAGACTCT+AGG 0.538710 5:+432953 None:intergenic
ACGCCAACGGTAGCGATGAC+CGG 0.540694 5:-436505 MsG0580024068.01.T01:CDS
TGACGGCACCGGCGAAGCAC+AGG 0.542644 5:-436411 MsG0580024068.01.T01:CDS
ACAGAGTCAGACACATTGCC+AGG 0.542707 5:+433792 None:intergenic
TTCCAGTAATCGCAACCAAA+CGG 0.543100 5:+435925 None:intergenic
TCTCTTCCGAGAACGGTGGA+AGG 0.543564 5:+436302 None:intergenic
TTCAACAGTGTCTTCATCAG+AGG 0.545221 5:+435797 None:intergenic
GAAGTCAATTTATCAAGCGC+AGG 0.545824 5:+435993 None:intergenic
GCTGAGATATCGTTGGAAGC+GGG 0.545841 5:-435717 MsG0580024068.01.T01:CDS
GTGCATTATTTATCTGACAT+GGG 0.547133 5:-432519 MsG0580024068.01.T01:three_prime_UTR
AAGTAACATCATGTTAGAGC+TGG 0.551688 5:+432485 None:intergenic
CACCACTAAGCTTGATGTTG+AGG 0.552846 5:-435568 MsG0580024068.01.T01:CDS
GGAGAGGGTCCAGGATTCGA+AGG 0.557929 5:+436333 None:intergenic
TTTCTTCCGAATTGCCGGTA+CGG 0.558235 5:+436463 None:intergenic
CCATGTGACAGTTAGATGAC+TGG 0.559072 5:+433079 None:intergenic
AGCAAATTAGTCCAAAGTCA+TGG 0.560002 5:+432440 None:intergenic
AAGATGCGAGGAAACGTCAC+CGG 0.560599 5:-436530 MsG0580024068.01.T01:CDS
ATGGGATGAGAGAGTTTATG+TGG 0.562365 5:-434303 MsG0580024068.01.T01:CDS
TGGTATAGGTCTTAAAGTCA+GGG 0.563205 5:+433490 None:intergenic
CCACCGGTCATCGCTACCGT+TGG 0.563218 5:+436502 None:intergenic
CTCTTGAACGAAAGGAGTAA+CGG 0.563746 5:+436196 None:intergenic
TCGTTGGAAGCGGGTTTAGG+AGG 0.564325 5:-435708 MsG0580024068.01.T01:CDS
GAAGCACAGGTAATCGAGGC+CGG 0.564508 5:-436398 MsG0580024068.01.T01:CDS
TTATAAGGTGTAGTTACAGA+AGG 0.565956 5:+433035 None:intergenic
GCTTACTTTGTTCAGATCGA+CGG 0.566014 5:-435525 MsG0580024068.01.T01:CDS
GTGAATTCTTAGCTCTCACA+TGG 0.566498 5:-432640 MsG0580024068.01.T01:three_prime_UTR
CAATACTTGTAGATATACCT+TGG 0.566714 5:+433529 None:intergenic
GGGAATGATATTACCAAACA+TGG 0.568231 5:+433238 None:intergenic
GCTTGAAGCTCTTGAACGAA+AGG 0.572210 5:+436188 None:intergenic
AGAGAGTTTATGTGGACTGT+TGG 0.574329 5:-434295 MsG0580024068.01.T01:CDS
CTCTTCCGAGAACGGTGGAA+GGG 0.575974 5:+436303 None:intergenic
TCAAGCGCAACGAATTCAAA+CGG 0.576753 5:+436083 None:intergenic
CAAGGATATTCTGATACCAT+GGG 0.576868 5:+433754 None:intergenic
GTACCGGCAATTCGGAAGAA+AGG 0.580132 5:-436461 MsG0580024068.01.T01:CDS
TGGAAGGGTAGGAGAGGAGA+GGG 0.581421 5:+436318 None:intergenic
ACCAGGTGCTGAGATATCGT+TGG 0.584563 5:-435724 MsG0580024068.01.T01:CDS
AGGAGAGGTGAATTATGAGG+AGG 0.586365 5:-435685 MsG0580024068.01.T01:CDS
GAGGCCGGTACGACGCATGA+TGG 0.587077 5:+436367 None:intergenic
CGGCGGCAGCTGTTCCCCAC+CGG 0.591719 5:+436486 None:intergenic
GGATGTCATGATGCCAAGGT+AGG 0.591991 5:-432883 MsG0580024068.01.T01:three_prime_UTR
CAACGGTAGCGATGACCGGT+GGG 0.594792 5:-436501 MsG0580024068.01.T01:CDS
GGAGAGTGAAGCAAAGACGC+TGG 0.597867 5:-436030 MsG0580024068.01.T01:CDS
AGCAAAGACGCTGGAACAAG+AGG 0.602892 5:-436021 MsG0580024068.01.T01:CDS
CTTCCGAATTGCCGGTACGG+CGG 0.605828 5:+436466 None:intergenic
AAACAAACATAATGTACACT+GGG 0.610252 5:+433948 None:intergenic
CACTCTCAATAAGCTCTCAA+CGG 0.611809 5:-435502 MsG0580024068.01.T01:intron
GGATTCGAAGGTCACGTGCA+GGG 0.614484 5:+436345 None:intergenic
CTTCGGATGTCATGATGCCA+AGG 0.614627 5:-432887 MsG0580024068.01.T01:three_prime_UTR
ATTATCAGGACGAGGAGTAG+TGG 0.614768 5:+436139 None:intergenic
TTTATGTGGACTGTTGGTGG+CGG 0.614954 5:-434289 MsG0580024068.01.T01:CDS
CATTACTCAAATTGAAACTG+TGG 0.618129 5:-432586 MsG0580024068.01.T01:three_prime_UTR
TTCCATGAGTTTGAGGACCA+AGG 0.618441 5:-433546 MsG0580024068.01.T01:three_prime_UTR
TCCAACGATATCTCAGCACC+TGG 0.620597 5:+435723 None:intergenic
AACGTCACCGGTAACGCCAA+CGG 0.620696 5:-436518 MsG0580024068.01.T01:CDS
TCCGAGAACGGTGGAAGGGT+AGG 0.621308 5:+436307 None:intergenic
TGTTTGATGATAAATTGTAT+GGG 0.621429 5:-434321 MsG0580024068.01.T01:CDS
GAACGGTGGAAGGGTAGGAG+AGG 0.622247 5:+436312 None:intergenic
CCAACGGTAGCGATGACCGG+TGG 0.624688 5:-436502 MsG0580024068.01.T01:CDS
CCAGTCATCTAACTGTCACA+TGG 0.627545 5:-433079 MsG0580024068.01.T01:three_prime_UTR
GTTGACCACGGCAACTCTGG+TGG 0.627812 5:-434398 MsG0580024068.01.T01:CDS
CGTTGATCCCCTAAACACAA+TGG 0.628459 5:+432412 None:intergenic
CTCAACATCAAGCTTAGTGG+TGG 0.628847 5:+435569 None:intergenic
GAGAAAATCAATTCTCACGA+GGG 0.628853 5:+433392 None:intergenic
TCTTCCTGATTATCAGGACG+AGG 0.628896 5:+436131 None:intergenic
TAGTGCTTGCACGTGTTCCG+CGG 0.631370 5:+435604 None:intergenic
CGGCGAAGCACAGGTAATCG+AGG 0.643846 5:-436402 MsG0580024068.01.T01:CDS
AAAATCAATTCTCACGAGGG+TGG 0.644396 5:+433395 None:intergenic
GTGGAAGGGTAGGAGAGGAG+AGG 0.646101 5:+436317 None:intergenic
TGAACGAAAGGAGTAACGGA+AGG 0.650529 5:+436200 None:intergenic
ACTTACCACCAGAGTTGCCG+TGG 0.664332 5:+434393 None:intergenic
GTCATGGAAAGAGTAAGACC+AGG 0.665566 5:+432456 None:intergenic
AATACCAGCGACAACAACGA+AGG 0.666521 5:+434369 None:intergenic
GATGTTAATCAAACTGGACC+AGG 0.673948 5:-435741 MsG0580024068.01.T01:CDS
AACGGTAGCGATGACCGGTG+GGG 0.675836 5:-436500 MsG0580024068.01.T01:CDS
GATAATCAGGAAGATTCTCA+CGG 0.676638 5:-436122 MsG0580024068.01.T01:CDS
GGAGAAAATCAATTCTCACG+AGG 0.677546 5:+433391 None:intergenic
GGTATAGGTCTTAAAGTCAG+GGG 0.678975 5:+433491 None:intergenic
GAAGCAAATAGATGTCGTAG+AGG 0.679419 5:+434107 None:intergenic
TCTTTCTCTTCCGAGAACGG+TGG 0.682175 5:+436298 None:intergenic
ACATTATTTATGTAGCACAA+CGG 0.683769 5:+434188 None:intergenic
AATGGGAGTCATGTTGACCA+CGG 0.693986 5:-434410 MsG0580024068.01.T01:CDS
TTCGAAGGTCACGTGCAGGG+AGG 0.702733 5:+436348 None:intergenic
TCGAATTTAAGGGATAGCCG+CGG 0.713074 5:-435621 MsG0580024068.01.T01:CDS
TGAATTCTTAGCTCTCACAT+GGG 0.716305 5:-432639 MsG0580024068.01.T01:three_prime_UTR
TGATGATGATGAAGATATGG+CGG 0.716447 5:-435865 MsG0580024068.01.T01:CDS
CGGCGACTAGAAAATTCCAG+TGG 0.717479 5:-433700 MsG0580024068.01.T01:three_prime_UTR
GGAGTATGTTGATGACACAG+AGG 0.741575 5:-434479 MsG0580024068.01.T01:CDS
AATCAGGAAGATTCTCACGG+CGG 0.760544 5:-436119 MsG0580024068.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATACGAGTATATATTA+TGG - Chr5:434132-434151 MsG0580024068.01.T01:three_prime_UTR 15.0%
!! AAAGTCAATAAGAATTTATA+AGG + Chr5:435774-435793 None:intergenic 15.0%
!! GAAAATATATTAGAAAGAAA+AGG + Chr5:435883-435902 None:intergenic 15.0%
!! TTTAATATAAAAAATAGGAG+AGG + Chr5:435481-435500 None:intergenic 15.0%
!!! AATTACAATTTTACAGAAAA+CGG + Chr5:434655-434674 None:intergenic 15.0%
!!! TTTCTAATATATTTTCTCTT+CGG - Chr5:435887-435906 MsG0580024068.01.T01:CDS 15.0%
!! AAATCAAGGAAATGTTAAAT+AGG + Chr5:433322-433341 None:intergenic 20.0%
!! AAGCTTCTTTAAACAATATT+TGG - Chr5:434904-434923 MsG0580024068.01.T01:intron 20.0%
!! GAATTCAAGAGAAAAAAATA+TGG + Chr5:435424-435443 None:intergenic 20.0%
!! GATAAATTATAATCAATCCA+AGG + Chr5:433828-433847 None:intergenic 20.0%
!! TATGAAAATTGTATCTTCAT+AGG + Chr5:433858-433877 None:intergenic 20.0%
!!! AATTCTTATTGACTTTGAAT+TGG - Chr5:435778-435797 MsG0580024068.01.T01:CDS 20.0%
!!! TGATTTTTCATAAACTAACT+TGG - Chr5:433915-433934 MsG0580024068.01.T01:three_prime_UTR 20.0%
!!! TGTTTGATGATAAATTGTAT+GGG - Chr5:434470-434489 MsG0580024068.01.T01:CDS 20.0%
!!! TTCGTATAAAATTTAAGTTC+AGG - Chr5:436555-436574 MsG0580024068.01.T01:five_prime_UTR 20.0%
!!! TTTAACATTTCCTTGATTTT+AGG - Chr5:433323-433342 MsG0580024068.01.T01:three_prime_UTR 20.0%
! AAAAAATAAGCCAATCCAAA+AGG + Chr5:433657-433676 None:intergenic 25.0%
! AAAAATAAGCCAATCCAAAA+GGG + Chr5:433656-433675 None:intergenic 25.0%
! AAAACAAACATAATGTACAC+TGG + Chr5:434847-434866 None:intergenic 25.0%
! AAACAAACATAATGTACACT+GGG + Chr5:434846-434865 None:intergenic 25.0%
! AAGAATATTTAGGCAGATTA+AGG - Chr5:436029-436048 MsG0580024068.01.T01:CDS 25.0%
! AATAATGCACCAAAAATTCT+TGG + Chr5:436262-436281 None:intergenic 25.0%
! AATATGCAAAAATCTGGTAT+AGG + Chr5:435318-435337 None:intergenic 25.0%
! AATTGTTTACTGTACAATAG+TGG + Chr5:436451-436470 None:intergenic 25.0%
! ACATTATTTATGTAGCACAA+CGG + Chr5:434606-434625 None:intergenic 25.0%
! ATATATTATGGTACAATACC+TGG - Chr5:434144-434163 MsG0580024068.01.T01:three_prime_UTR 25.0%
! ATCATTGAAGAGAAAAAGAA+AGG + Chr5:435511-435530 None:intergenic 25.0%
! CAATAGCAATTATATAAACC+AGG + Chr5:434165-434184 None:intergenic 25.0%
! CCTTGAACAATGTTATAAAT+GGG - Chr5:435055-435074 MsG0580024068.01.T01:intron 25.0%
! GTACAAGAAAAATTAGTTGA+AGG + Chr5:434725-434744 None:intergenic 25.0%
! TATGTGATATGATGTTACTA+TGG - Chr5:434191-434210 MsG0580024068.01.T01:three_prime_UTR 25.0%
! TCCTTGAACAATGTTATAAA+TGG - Chr5:435054-435073 MsG0580024068.01.T01:intron 25.0%
!! AACAATGTTATAAATGGGTT+GGG - Chr5:435060-435079 MsG0580024068.01.T01:intron 25.0%
!! CTGTTTGATGATAAATTGTA+TGG - Chr5:434469-434488 MsG0580024068.01.T01:CDS 25.0%
!! TTTTCTTGTACATTGTTGTA+TGG - Chr5:434734-434753 MsG0580024068.01.T01:intron 25.0%
!!! CATTAGTGTTTTTAACTTGT+TGG + Chr5:433382-433401 None:intergenic 25.0%
!!! CTATTCTGGTTTATAAACTT+TGG - Chr5:434973-434992 MsG0580024068.01.T01:intron 25.0%
!!! GTAACTTTAGGAGATATTTT+AGG - Chr5:436401-436420 MsG0580024068.01.T01:CDS 25.0%
AAATCAGCTTATGAAGTAGT+AGG - Chr5:433753-433772 MsG0580024068.01.T01:three_prime_UTR 30.0%
AATAGGAGAGGAAATGTTTA+AGG + Chr5:435469-435488 None:intergenic 30.0%
ACCTACCTAAAAGTAATGTT+TGG + Chr5:436124-436143 None:intergenic 30.0%
AGAATAGCAATTAAGAGAAG+TGG + Chr5:434960-434979 None:intergenic 30.0%
AGCTGAACTACTGAATAATT+TGG - Chr5:436090-436109 MsG0580024068.01.T01:CDS 30.0%
ATGTCACTAGATAAGTTCAA+GGG - Chr5:433630-433649 MsG0580024068.01.T01:three_prime_UTR 30.0%
ATTCAGATACGTGATTTCAT+TGG + Chr5:435675-435694 None:intergenic 30.0%
CAAGGAAATGTTAAATAGGA+AGG + Chr5:433318-433337 None:intergenic 30.0%
CAATACTTGTAGATATACCT+TGG + Chr5:435265-435284 None:intergenic 30.0%
CACAGTTTCAATTTGAGTAA+TGG + Chr5:436207-436226 None:intergenic 30.0%
CATTACTCAAATTGAAACTG+TGG - Chr5:436205-436224 MsG0580024068.01.T01:CDS 30.0%
CCCATTTATAACATTGTTCA+AGG + Chr5:435058-435077 None:intergenic 30.0%
CGAATTCCACACAAAAAATT+GGG - Chr5:432195-432214 MsG0580024068.01.T01:three_prime_UTR 30.0%
CTTAGTGGCAAAGAATATTT+AGG - Chr5:436019-436038 MsG0580024068.01.T01:CDS 30.0%
CTTATTTCCATAAACTCACT+AGG + Chr5:433541-433560 None:intergenic 30.0%
GAACAATGTTATAAATGGGT+TGG - Chr5:435059-435078 MsG0580024068.01.T01:intron 30.0%
GAAGATTAGTACTCTCAATT+TGG - Chr5:432821-432840 MsG0580024068.01.T01:three_prime_UTR 30.0%
GAGAAGAATATGCAAAAATC+TGG + Chr5:435324-435343 None:intergenic 30.0%
GATCATGATGTTCATGATAA+TGG - Chr5:433023-433042 MsG0580024068.01.T01:three_prime_UTR 30.0%
GTGCATTATTTATCTGACAT+GGG - Chr5:436272-436291 MsG0580024068.01.T01:CDS 30.0%
GTTGTAGAAACCTAAAATCA+AGG + Chr5:433336-433355 None:intergenic 30.0%
TATGTCACTAGATAAGTTCA+AGG - Chr5:433629-433648 MsG0580024068.01.T01:three_prime_UTR 30.0%
TATTCAATGATCTCCATGTT+TGG - Chr5:435540-435559 MsG0580024068.01.T01:CDS 30.0%
TCAAAAAGTGAGAGATGAAT+TGG - Chr5:432893-432912 MsG0580024068.01.T01:three_prime_UTR 30.0%
TCGAATTCCACACAAAAAAT+TGG - Chr5:432194-432213 MsG0580024068.01.T01:three_prime_UTR 30.0%
TGATACAGTGTACTAGTAAT+CGG + Chr5:435988-436007 None:intergenic 30.0%
TTATAAGGTGTAGTTACAGA+AGG + Chr5:435759-435778 None:intergenic 30.0%
TTATCATGAACATCATGATC+AGG + Chr5:433023-433042 None:intergenic 30.0%
! AATTCTAGAGAGCAAATGTT+TGG - Chr5:433143-433162 MsG0580024068.01.T01:three_prime_UTR 30.0%
! ACTTCTCTTAATTGCTATTC+TGG - Chr5:434959-434978 MsG0580024068.01.T01:intron 30.0%
! CTCTTTGGATTGACTTATTT+GGG + Chr5:434025-434044 None:intergenic 30.0%
! GTTTGTACTCCAAGAATTTT+TGG - Chr5:436250-436269 MsG0580024068.01.T01:CDS 30.0%
! TCTCTTTGGATTGACTTATT+TGG + Chr5:434026-434045 None:intergenic 30.0%
! TCTTTGGATTGACTTATTTG+GGG + Chr5:434024-434043 None:intergenic 30.0%
! TCTTTTTCTCTTCAATGATC+TGG - Chr5:435512-435531 MsG0580024068.01.T01:CDS 30.0%
! TTTTTGCTTTCTGATCTAGT+TGG - Chr5:433421-433440 MsG0580024068.01.T01:three_prime_UTR 30.0%
!! ACCAAACATTACTTTTAGGT+AGG - Chr5:436120-436139 MsG0580024068.01.T01:CDS 30.0%
!! ATATTTGTTTTCTCTTCCAC+TGG + Chr5:435110-435129 None:intergenic 30.0%
!! CACTTTTAAGTCAGAAAGTT+TGG + Chr5:436516-436535 None:intergenic 30.0%
!! GCTGATACTTTGTTATTTGT+TGG - Chr5:434270-434289 MsG0580024068.01.T01:CDS 30.0%
!! TGTGACCAAACATTACTTTT+AGG - Chr5:436116-436135 MsG0580024068.01.T01:CDS 30.0%
!!! CTCTAACATGATGTTACTTT+TGG - Chr5:436310-436329 MsG0580024068.01.T01:CDS 30.0%
AAAAATAAGCCGATCCAAAC+GGG - Chr5:433997-434016 MsG0580024068.01.T01:three_prime_UTR 35.0%
AAGTAACATCATGTTAGAGC+TGG + Chr5:436309-436328 None:intergenic 35.0%
AATGTGTCTGACTCTGTTTA+TGG - Chr5:435005-435024 MsG0580024068.01.T01:intron 35.0%
AGAAAAGGAAACACGAAGAA+TGG + Chr5:435868-435887 None:intergenic 35.0%
AGCAAATTAGTCCAAAGTCA+TGG + Chr5:436354-436373 None:intergenic 35.0%
AGCAACCACATACAAGAATA+TGG + Chr5:434539-434558 None:intergenic 35.0%
ATATTCTTTGCCACTAAGAC+AGG + Chr5:436017-436036 None:intergenic 35.0%
ATCAAAATGACCTGTCTTAG+TGG - Chr5:436004-436023 MsG0580024068.01.T01:CDS 35.0%
ATGCTATCCTAGTGAGTTTA+TGG - Chr5:433531-433550 MsG0580024068.01.T01:three_prime_UTR 35.0%
CAAAAATAAGCCGATCCAAA+CGG - Chr5:433996-434015 MsG0580024068.01.T01:three_prime_UTR 35.0%
CAAGGATATTCTGATACCAT+GGG + Chr5:435040-435059 None:intergenic 35.0%
CAGAAAAAATCAAAGATGCG+AGG - Chr5:432249-432268 MsG0580024068.01.T01:three_prime_UTR 35.0%
CTAACTGTCACATGGTATTA+TGG - Chr5:435720-435739 MsG0580024068.01.T01:CDS 35.0%
GAAAAGGAAACACGAAGAAT+GGG + Chr5:435867-435886 None:intergenic 35.0%
GAGAAAATCAATTCTCACGA+GGG + Chr5:435402-435421 None:intergenic 35.0%
GATAATCAGGAAGATTCTCA+CGG - Chr5:432669-432688 MsG0580024068.01.T01:three_prime_UTR 35.0%
GGGAATGATATTACCAAACA+TGG + Chr5:435556-435575 None:intergenic 35.0%
GGTGCATTATTTATCTGACA+TGG - Chr5:436271-436290 MsG0580024068.01.T01:CDS 35.0%
TCAAGGATATTCTGATACCA+TGG + Chr5:435041-435060 None:intergenic 35.0%
TCTTACTCTTTCCATGACTT+TGG - Chr5:436340-436359 MsG0580024068.01.T01:CDS 35.0%
TGAGAATCTTCCTGATTATC+AGG + Chr5:432669-432688 None:intergenic 35.0%
TGGTATAGGTCTTAAAGTCA+GGG + Chr5:435304-435323 None:intergenic 35.0%
TTCATTTCTGCTGTATTCTC+TGG - Chr5:435614-435633 MsG0580024068.01.T01:CDS 35.0%
TTGCAAGAAAAAACCTACCT+TGG + Chr5:435924-435943 None:intergenic 35.0%
! ATATGAGAGAAGTTTTCACG+TGG + Chr5:435165-435184 None:intergenic 35.0%
! CAAAGTATCAGCAACAAAGA+AGG + Chr5:434262-434281 None:intergenic 35.0%
! CACTTTATGGTTTGTACTTG+AGG - Chr5:433802-433821 MsG0580024068.01.T01:three_prime_UTR 35.0%
! GTTTATAAACTTTGGAAGCC+TGG - Chr5:434981-435000 MsG0580024068.01.T01:intron 35.0%
! TGAATTCTTAGCTCTCACAT+GGG - Chr5:436152-436171 MsG0580024068.01.T01:CDS 35.0%
! TGATGATGATGAAGATATGG+CGG - Chr5:432926-432945 MsG0580024068.01.T01:three_prime_UTR 35.0%
!! GCTTGATGATGATGAAGATA+TGG - Chr5:432923-432942 MsG0580024068.01.T01:three_prime_UTR 35.0%
!! GGTGTTGATGTTAATCAAAC+TGG - Chr5:433044-433063 MsG0580024068.01.T01:three_prime_UTR 35.0%
!! GTTTGGTGCTTCGAATTTAA+GGG - Chr5:433160-433179 MsG0580024068.01.T01:three_prime_UTR 35.0%
!! TGTTTGGTGCTTCGAATTTA+AGG - Chr5:433159-433178 MsG0580024068.01.T01:three_prime_UTR 35.0%
!! TTTATGGCTTTATATGCCCA+TGG - Chr5:435021-435040 MsG0580024068.01.T01:intron 35.0%
!!! ATGATGTTACTTTTGGTTCC+TGG - Chr5:436317-436336 MsG0580024068.01.T01:CDS 35.0%
!!! GTGTTTTTAACTTGTTGGAC+TGG + Chr5:433377-433396 None:intergenic 35.0%
!!! TTCGTTCCCAATTTTTTGTG+TGG + Chr5:432204-432223 None:intergenic 35.0%
AAAATCAATTCTCACGAGGG+TGG + Chr5:435399-435418 None:intergenic 40.0%
AAGTACACACGCACTTACTT+TGG + Chr5:436485-436504 None:intergenic 40.0%
ACACGAAGAATGGGATTGAT+AGG + Chr5:435858-435877 None:intergenic 40.0%
ACAGAACCATCTTCTCCAAA+TGG - Chr5:434363-434382 MsG0580024068.01.T01:CDS 40.0%
ATGGGATGAGAGAGTTTATG+TGG - Chr5:434488-434507 MsG0580024068.01.T01:intron 40.0%
CACTCTCAATAAGCTCTCAA+CGG - Chr5:433289-433308 MsG0580024068.01.T01:three_prime_UTR 40.0%
CAGAACCATCTTCTCCAAAT+GGG - Chr5:434364-434383 MsG0580024068.01.T01:CDS 40.0%
CATAGTCTTCCATGAGTTTG+AGG - Chr5:435238-435257 MsG0580024068.01.T01:intron 40.0%
CTAGAGTCCATTGTGTTTAG+GGG - Chr5:436372-436391 MsG0580024068.01.T01:CDS 40.0%
CTCTTGAACGAAAGGAGTAA+CGG + Chr5:432598-432617 None:intergenic 40.0%
CTGGTATAGGTCTTAAAGTC+AGG + Chr5:435305-435324 None:intergenic 40.0%
GAAGCAAATAGATGTCGTAG+AGG + Chr5:434687-434706 None:intergenic 40.0%
GAAGTCAATTTATCAAGCGC+AGG + Chr5:432801-432820 None:intergenic 40.0%
GAAGTCACTTCAACAGCAAT+TGG - Chr5:432965-432984 MsG0580024068.01.T01:three_prime_UTR 40.0%
GACAGAGATGTTCAATCTGA+TGG + Chr5:435199-435218 None:intergenic 40.0%
GAGAAGAAGAACTTCGTTTG+CGG + Chr5:432559-432578 None:intergenic 40.0%
GATAGGTCTATGAAGACTCT+AGG + Chr5:435841-435860 None:intergenic 40.0%
GATGTTAATCAAACTGGACC+AGG - Chr5:433050-433069 MsG0580024068.01.T01:three_prime_UTR 40.0%
GCTAGAGTCCATTGTGTTTA+GGG - Chr5:436371-436390 MsG0580024068.01.T01:CDS 40.0%
GCTTACTTTGTTCAGATCGA+CGG - Chr5:433266-433285 MsG0580024068.01.T01:three_prime_UTR 40.0%
GGAGAAAATCAATTCTCACG+AGG + Chr5:435403-435422 None:intergenic 40.0%
GGTATAGGTCTTAAAGTCAG+GGG + Chr5:435303-435322 None:intergenic 40.0%
GTTTGTACTAGAGTCACAAC+AGG - Chr5:435370-435389 MsG0580024068.01.T01:intron 40.0%
TAACATCATGTTAGAGCTGG+TGG + Chr5:436306-436325 None:intergenic 40.0%
TAAGTGCGTGTGTACTTTGA+AGG - Chr5:436488-436507 MsG0580024068.01.T01:CDS 40.0%
TAGGGGATCAACGTAACTTT+AGG - Chr5:436389-436408 MsG0580024068.01.T01:CDS 40.0%
TCAAGCGCAACGAATTCAAA+CGG + Chr5:432711-432730 None:intergenic 40.0%
TGCTAGAGTCCATTGTGTTT+AGG - Chr5:436370-436389 MsG0580024068.01.T01:CDS 40.0%
TGGGACCATATTCTTGTATG+TGG - Chr5:434531-434550 MsG0580024068.01.T01:intron 40.0%
TTCAACAGTGTCTTCATCAG+AGG + Chr5:432997-433016 None:intergenic 40.0%
TTCCAGTAATCGCAACCAAA+CGG + Chr5:432869-432888 None:intergenic 40.0%
TTTGGTTGCGATTACTGGAA+GGG - Chr5:432869-432888 MsG0580024068.01.T01:three_prime_UTR 40.0%
! AGAGAGTTTATGTGGACTGT+TGG - Chr5:434496-434515 MsG0580024068.01.T01:intron 40.0%
! GTGAATTCTTAGCTCTCACA+TGG - Chr5:436151-436170 MsG0580024068.01.T01:CDS 40.0%
! GTTGTTGTCGCTGGTATTTT+TGG - Chr5:434427-434446 MsG0580024068.01.T01:CDS 40.0%
! TGAAGCAGCTTGTAGTGTTT+TGG - Chr5:432740-432759 MsG0580024068.01.T01:three_prime_UTR 40.0%
!! ATCATTCCCTTTTAACTGCC+AGG - Chr5:435567-435586 MsG0580024068.01.T01:CDS 40.0%
!! TCATTCCCTTTTAACTGCCA+GGG - Chr5:435568-435587 MsG0580024068.01.T01:CDS 40.0%
AAGGTACAAGCGTTCGTCAA+TGG - Chr5:432349-432368 MsG0580024068.01.T01:three_prime_UTR 45.0%
AATACCAGCGACAACAACGA+AGG + Chr5:434425-434444 None:intergenic 45.0%
AATCAGGAAGATTCTCACGG+CGG - Chr5:432672-432691 MsG0580024068.01.T01:three_prime_UTR 45.0%
AATGGGAGTCATGTTGACCA+CGG - Chr5:434381-434400 MsG0580024068.01.T01:CDS 45.0%
AGGAGAGGTGAATTATGAGG+AGG - Chr5:433106-433125 MsG0580024068.01.T01:three_prime_UTR 45.0%
AGGAGGAGAGGTGAATTATG+AGG - Chr5:433103-433122 MsG0580024068.01.T01:three_prime_UTR 45.0%
ATTATCAGGACGAGGAGTAG+TGG + Chr5:432655-432674 None:intergenic 45.0%
CACCACTAAGCTTGATGTTG+AGG - Chr5:433223-433242 MsG0580024068.01.T01:three_prime_UTR 45.0%
CCAGTCATCTAACTGTCACA+TGG - Chr5:435712-435731 MsG0580024068.01.T01:CDS 45.0%
CCATGTGACAGTTAGATGAC+TGG + Chr5:435715-435734 None:intergenic 45.0%
CGTTGATCCCCTAAACACAA+TGG + Chr5:436382-436401 None:intergenic 45.0%
CTCAACATCAAGCTTAGTGG+TGG + Chr5:433225-433244 None:intergenic 45.0%
CTCCTCAACATCAAGCTTAG+TGG + Chr5:433228-433247 None:intergenic 45.0%
GCTTGAAGCTCTTGAACGAA+AGG + Chr5:432606-432625 None:intergenic 45.0%
GGTGAATTATGAGGAGGATG+AGG - Chr5:433112-433131 MsG0580024068.01.T01:three_prime_UTR 45.0%
GTACCTTTCTTCCGAATTGC+CGG + Chr5:432336-432355 None:intergenic 45.0%
GTCATGGAAAGAGTAAGACC+AGG + Chr5:436338-436357 None:intergenic 45.0%
GTCGGTACTAAGAGTCTCTT+TGG + Chr5:434040-434059 None:intergenic 45.0%
GTTTGGTTGCGATTACTGGA+AGG - Chr5:432868-432887 MsG0580024068.01.T01:three_prime_UTR 45.0%
TACCTTGGTCCTCAAACTCA+TGG + Chr5:435250-435269 None:intergenic 45.0%
TACTCCTCGTCCTGATAATC+AGG - Chr5:432656-432675 MsG0580024068.01.T01:three_prime_UTR 45.0%
TCGAATTTAAGGGATAGCCG+CGG - Chr5:433170-433189 MsG0580024068.01.T01:three_prime_UTR 45.0%
TCGTCAGATTAAGAGCCGTT+TGG - Chr5:432851-432870 MsG0580024068.01.T01:three_prime_UTR 45.0%
TCTTCCTGATTATCAGGACG+AGG + Chr5:432663-432682 None:intergenic 45.0%
TGACTCCCATTTGGAGAAGA+TGG + Chr5:434372-434391 None:intergenic 45.0%
TGCTCTTTCTCTTCCGAGAA+CGG + Chr5:432499-432518 None:intergenic 45.0%
TGCTGAGATATCGTTGGAAG+CGG - Chr5:433073-433092 MsG0580024068.01.T01:three_prime_UTR 45.0%
TGGTCAACATGACTCCCATT+TGG + Chr5:434381-434400 None:intergenic 45.0%
TTCCATGAGTTTGAGGACCA+AGG - Chr5:435245-435264 MsG0580024068.01.T01:intron 45.0%
TTTCTTCCGAATTGCCGGTA+CGG + Chr5:432331-432350 None:intergenic 45.0%
! ATATCGTTGGAAGCGGGTTT+AGG - Chr5:433080-433099 MsG0580024068.01.T01:three_prime_UTR 45.0%
! ATGGTTTGTACTTGAGGCCT+TGG - Chr5:433808-433827 MsG0580024068.01.T01:three_prime_UTR 45.0%
! GACTTGATGGTCTGTACTTG+AGG - Chr5:434074-434093 MsG0580024068.01.T01:three_prime_UTR 45.0%
! GAGTTTATGTGGACTGTTGG+TGG - Chr5:434499-434518 MsG0580024068.01.T01:intron 45.0%
! GATAAGTTCAAGGGCCCTTT+TGG - Chr5:433639-433658 MsG0580024068.01.T01:three_prime_UTR 45.0%
! GGTTGTCTTGTTGAAGCTGA+GGG - Chr5:434291-434310 MsG0580024068.01.T01:CDS 45.0%
! TGAACGAAAGGAGTAACGGA+AGG + Chr5:432594-432613 None:intergenic 45.0%
! TGGTTGTCTTGTTGAAGCTG+AGG - Chr5:434290-434309 MsG0580024068.01.T01:CDS 45.0%
! TGTATGTGGTTGCTATTGCC+TGG - Chr5:434545-434564 MsG0580024068.01.T01:intron 45.0%
! TTCGTGATGACGAAGAATCC+TGG + Chr5:432628-432647 None:intergenic 45.0%
! TTTATGTGGACTGTTGGTGG+CGG - Chr5:434502-434521 MsG0580024068.01.T01:intron 45.0%
!! AAATGGGTTGGGATCCTCTT+CGG - Chr5:435071-435090 MsG0580024068.01.T01:intron 45.0%
!! CATTCCCTTTTAACTGCCAG+GGG - Chr5:435569-435588 MsG0580024068.01.T01:CDS 45.0%
!! GAATTTTCTAGTCGCCGAAG+AGG + Chr5:435088-435107 None:intergenic 45.0%
!! GGAGTATGTTGATGACACAG+AGG - Chr5:434312-434331 MsG0580024068.01.T01:CDS 45.0%
!!! TGATTTTTAATATAAAAAAT+AGG + Chr5:435486-435505 None:intergenic 5.0%
AAGATGCGAGGAAACGTCAC+CGG - Chr5:432261-432280 MsG0580024068.01.T01:three_prime_UTR 50.0%
ACAGAGTCAGACACATTGCC+AGG + Chr5:435002-435021 None:intergenic 50.0%
ACCATCAAGTCTTCTCCTCG+CGG + Chr5:434065-434084 None:intergenic 50.0%
AGCAAAGACGCTGGAACAAG+AGG - Chr5:432770-432789 MsG0580024068.01.T01:three_prime_UTR 50.0%
AGCCGTTTGGTTGCGATTAC+TGG - Chr5:432864-432883 MsG0580024068.01.T01:three_prime_UTR 50.0%
ATGGTCTGTACTTGAGGCCT+TGG - Chr5:434080-434099 MsG0580024068.01.T01:three_prime_UTR 50.0%
ATTCTCATTTGAGCTGCCCC+TGG + Chr5:435588-435607 None:intergenic 50.0%
CGGCGACTAGAAAATTCCAG+TGG - Chr5:435091-435110 MsG0580024068.01.T01:intron 50.0%
CTTCGGATGTCATGATGCCA+AGG - Chr5:435904-435923 MsG0580024068.01.T01:CDS 50.0%
GCTGAGATATCGTTGGAAGC+GGG - Chr5:433074-433093 MsG0580024068.01.T01:three_prime_UTR 50.0%
GGATGTCATGATGCCAAGGT+AGG - Chr5:435908-435927 MsG0580024068.01.T01:CDS 50.0%
TACAAGCGTTCGTCAATGGC+TGG - Chr5:432353-432372 MsG0580024068.01.T01:three_prime_UTR 50.0%
TCCAACGATATCTCAGCACC+TGG + Chr5:433071-433090 None:intergenic 50.0%
TCGTTCAAGAGCTTCAAGCC+AGG - Chr5:432607-432626 MsG0580024068.01.T01:three_prime_UTR 50.0%
TCTTTCTCTTCCGAGAACGG+TGG + Chr5:432496-432515 None:intergenic 50.0%
TGAGGAGCTTGAGATGCTCT+TGG - Chr5:433241-433260 MsG0580024068.01.T01:three_prime_UTR 50.0%
! ACCATAAAGTGCTCTCCGCT+CGG + Chr5:433793-433812 None:intergenic 50.0%
! ATTTGGGGTCCCGTTTGGAT+CGG + Chr5:434009-434028 None:intergenic 50.0%
! CAAACGCTTGCTGGAGTGAA+TGG - Chr5:434576-434595 MsG0580024068.01.T01:intron 50.0%
! CGTCAATGGCTGGTTGTTGA+CGG - Chr5:432363-432382 MsG0580024068.01.T01:three_prime_UTR 50.0%
! GACTTATTTGGGGTCCCGTT+TGG + Chr5:434014-434033 None:intergenic 50.0%
! GTACCGGCAATTCGGAAGAA+AGG - Chr5:432330-432349 MsG0580024068.01.T01:three_prime_UTR 50.0%
! GTTCAAGGGCCCTTTTGGAT+TGG - Chr5:433644-433663 MsG0580024068.01.T01:three_prime_UTR 50.0%
!! ACCAGGTGCTGAGATATCGT+TGG - Chr5:433067-433086 MsG0580024068.01.T01:three_prime_UTR 50.0%
AACGTCACCGGTAACGCCAA+CGG - Chr5:432273-432292 MsG0580024068.01.T01:three_prime_UTR 55.0%
ACCGCGAGGAGAAGACTTGA+TGG - Chr5:434061-434080 MsG0580024068.01.T01:three_prime_UTR 55.0%
ACTTACCACCAGAGTTGCCG+TGG + Chr5:434401-434420 None:intergenic 55.0%
AGCTGCCCCTGGCAGTTAAA+AGG + Chr5:435577-435596 None:intergenic 55.0%
AGGATTCGAAGGTCACGTGC+AGG + Chr5:432450-432469 None:intergenic 55.0%
AGTCTTCTCCTCGCGGTAGT+CGG + Chr5:434058-434077 None:intergenic 55.0%
AGTGCCTTCGTTGTTGTCGC+TGG - Chr5:434418-434437 MsG0580024068.01.T01:CDS 55.0%
ATCGCTACCGTTGGCGTTAC+CGG + Chr5:432283-432302 None:intergenic 55.0%
CAAGCGTTTGTGCTTGCACC+AGG + Chr5:434566-434585 None:intergenic 55.0%
CATGTTGACCACGGCAACTC+TGG - Chr5:434390-434409 MsG0580024068.01.T01:CDS 55.0%
CTCGATTACCTGTGCTTCGC+CGG + Chr5:432391-432410 None:intergenic 55.0%
CTCTTCCGAGAACGGTGGAA+GGG + Chr5:432491-432510 None:intergenic 55.0%
GAAGCACAGGTAATCGAGGC+CGG - Chr5:432393-432412 MsG0580024068.01.T01:three_prime_UTR 55.0%
GCTGCCCCTGGCAGTTAAAA+GGG + Chr5:435576-435595 None:intergenic 55.0%
GGAGAGTGAAGCAAAGACGC+TGG - Chr5:432761-432780 MsG0580024068.01.T01:three_prime_UTR 55.0%
GGATTCGAAGGTCACGTGCA+GGG + Chr5:432449-432468 None:intergenic 55.0%
GTGCAAGCACAAACGCTTGC+TGG - Chr5:434567-434586 MsG0580024068.01.T01:intron 55.0%
TAGTGCTTGCACGTGTTCCG+CGG + Chr5:433190-433209 None:intergenic 55.0%
TCCTACCCTTCCACCGTTCT+CGG - Chr5:432483-432502 MsG0580024068.01.T01:three_prime_UTR 55.0%
TCTCTTCCGAGAACGGTGGA+AGG + Chr5:432492-432511 None:intergenic 55.0%
TGCACGTGACCTTCGAATCC+TGG - Chr5:432449-432468 MsG0580024068.01.T01:three_prime_UTR 55.0%
TGGAAGGGTAGGAGAGGAGA+GGG + Chr5:432476-432495 None:intergenic 55.0%
! ACGCATGATGGCGTGCTTTC+CGG + Chr5:432415-432434 None:intergenic 55.0%
! TCGTTGGAAGCGGGTTTAGG+AGG - Chr5:433083-433102 MsG0580024068.01.T01:three_prime_UTR 55.0%
! TTGGAAGCGGGTTTAGGAGG+AGG - Chr5:433086-433105 MsG0580024068.01.T01:three_prime_UTR 55.0%
!! ACCGAGCGGAGAGCACTTTA+TGG - Chr5:433789-433808 MsG0580024068.01.T01:three_prime_UTR 55.0%
!! GACAAGTACCGACTACCGAG+CGG - Chr5:433775-433794 MsG0580024068.01.T01:three_prime_UTR 55.0%
!! TCTTAGTACCGACTACCGCG+AGG - Chr5:434047-434066 MsG0580024068.01.T01:three_prime_UTR 55.0%
AACGGTAGCGATGACCGGTG+GGG - Chr5:432291-432310 MsG0580024068.01.T01:three_prime_UTR 60.0%
ACGCCAACGGTAGCGATGAC+CGG - Chr5:432286-432305 MsG0580024068.01.T01:three_prime_UTR 60.0%
AGTGCTCTCCGCTCGGTAGT+CGG + Chr5:433786-433805 None:intergenic 60.0%
CAACGGTAGCGATGACCGGT+GGG - Chr5:432290-432309 MsG0580024068.01.T01:three_prime_UTR 60.0%
CACGCCATCATGCGTCGTAC+CGG - Chr5:432420-432439 MsG0580024068.01.T01:three_prime_UTR 60.0%
CGGCGAAGCACAGGTAATCG+AGG - Chr5:432389-432408 MsG0580024068.01.T01:three_prime_UTR 60.0%
CTTCCGAATTGCCGGTACGG+CGG + Chr5:432328-432347 None:intergenic 60.0%
GAACGGTGGAAGGGTAGGAG+AGG + Chr5:432482-432501 None:intergenic 60.0%
GGAGAGGGTCCAGGATTCGA+AGG + Chr5:432461-432480 None:intergenic 60.0%
GTGGAAGGGTAGGAGAGGAG+AGG + Chr5:432477-432496 None:intergenic 60.0%
GTTGACCACGGCAACTCTGG+TGG - Chr5:434393-434412 MsG0580024068.01.T01:CDS 60.0%
TCCGAGAACGGTGGAAGGGT+AGG + Chr5:432487-432506 None:intergenic 60.0%
TTCGAAGGTCACGTGCAGGG+AGG + Chr5:432446-432465 None:intergenic 60.0%
! AGCGGGTTTAGGAGGAGGAG+AGG - Chr5:433091-433110 MsG0580024068.01.T01:three_prime_UTR 60.0%
! TGGCTGGTTGTTGACGGCAC+CGG - Chr5:432369-432388 MsG0580024068.01.T01:three_prime_UTR 60.0%
AAGGTCACGTGCAGGGAGGC+CGG + Chr5:432442-432461 None:intergenic 65.0%
CCAACGGTAGCGATGACCGG+TGG - Chr5:432289-432308 MsG0580024068.01.T01:three_prime_UTR 65.0%
CCACCGGTCATCGCTACCGT+TGG + Chr5:432292-432311 None:intergenic 65.0%
GAGGCCGGTACGACGCATGA+TGG + Chr5:432427-432446 None:intergenic 65.0%
GGTAGGAGAGGAGAGGGTCC+AGG + Chr5:432470-432489 None:intergenic 65.0%
!! ACTGTTGGTGGCGGCACTGC+TGG - Chr5:434511-434530 MsG0580024068.01.T01:intron 65.0%
!! CTGTTGGTGGCGGCACTGCT+GGG - Chr5:434512-434531 MsG0580024068.01.T01:intron 65.0%
AACAGCTGCCGCCGCCGTAC+CGG - Chr5:432314-432333 MsG0580024068.01.T01:three_prime_UTR 70.0%
CCGAATTGCCGGTACGGCGG+CGG + Chr5:432325-432344 None:intergenic 70.0%
! CCGCCGCCGTACCGGCAATT+CGG - Chr5:432322-432341 MsG0580024068.01.T01:three_prime_UTR 70.0%
!! TGACGGCACCGGCGAAGCAC+AGG - Chr5:432380-432399 MsG0580024068.01.T01:three_prime_UTR 70.0%
CGGCGGCAGCTGTTCCCCAC+CGG + Chr5:432308-432327 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 432190 436623 432190 ID=MsG0580024068.01;Name=MsG0580024068.01
Chr5 mRNA 432190 436623 432190 ID=MsG0580024068.01.T01;Parent=MsG0580024068.01;Name=MsG0580024068.01.T01;_AED=0.43;_eAED=0.43;_QI=74|1|1|1|1|1|2|2029|444
Chr5 exon 432190 434506 432190 ID=MsG0580024068.01.T01:exon:23076;Parent=MsG0580024068.01.T01
Chr5 exon 435503 436623 435503 ID=MsG0580024068.01.T01:exon:23075;Parent=MsG0580024068.01.T01
Chr5 five_prime_UTR 436550 436623 436550 ID=MsG0580024068.01.T01:five_prime_utr;Parent=MsG0580024068.01.T01
Chr5 CDS 435503 436549 435503 ID=MsG0580024068.01.T01:cds;Parent=MsG0580024068.01.T01
Chr5 CDS 434219 434506 434219 ID=MsG0580024068.01.T01:cds;Parent=MsG0580024068.01.T01
Chr5 three_prime_UTR 432190 434218 432190 ID=MsG0580024068.01.T01:three_prime_utr;Parent=MsG0580024068.01.T01
Gene Sequence

>MsG0580024068.01.T01

ATGCGAGGAAACGTCACCGGTAACGCCAACGGTAGCGATGACCGGTGGGGAACAGCTGCCGCCGCCGTACCGGCAATTCGGAAGAAAGGTACAAGCGTTCGTCAATGGCTGGTTGTTGACGGCACCGGCGAAGCACAGGTAATCGAGGCCGGAAAGCACGCCATCATGCGTCGTACCGGCCTCCCTGCACGTGACCTTCGAATCCTGGACCCTCTCCTCTCCTACCCTTCCACCGTTCTCGGAAGAGAAAGAGCAATCGTCATCAACTTAGAACACATCAAAGCAATTATCACCGCAAACGAAGTTCTTCTTCTCAATTCACGCGATCCTTCCGTTACTCCTTTCGTTCAAGAGCTTCAAGCCAGGATTCTTCGTCATCACGAAGCAACCACTACTCCTCGTCCTGATAATCAGGAAGATTCTCACGGCGGAATTAAGATTCTTCCGTTTGAATTCGTTGCGCTTGAAGCGTGTCTTGAAGCAGCTTGTAGTGTTTTGGAGAGTGAAGCAAAGACGCTGGAACAAGAGGCGCATCCTGCGCTTGATAAATTGACTTCGAAGATTAGTACTCTCAATTTGGAACGTGTTCGTCAGATTAAGAGCCGTTTGGTTGCGATTACTGGAAGGGTTCAAAAAGTGAGAGATGAATTGGAACACTTGCTTGATGATGATGAAGATATGGCGGAGATGTATCTTACTGAGAAGTCACTTCAACAGCAATTGGAACAAACCTCTGATGAAGACACTGTTGAAACTCCTGATCATGATGTTCATGATAATGGTGTTGATGTTAATCAAACTGGACCAGGTGCTGAGATATCGTTGGAAGCGGGTTTAGGAGGAGGAGAGGTGAATTATGAGGAGGATGAGGATAATCTGAATTCTAGAGAGCAAATGTTTGGTGCTTCGAATTTAAGGGATAGCCGCGGAACACGTGCAAGCACTACTTATAGTGTCACCACCACTAAGCTTGATGTTGAGGAGCTTGAGATGCTCTTGGAAGCTTACTTTGTTCAGATCGACGGCACTCTCAATAAGCTCTCAACGCTGAGGGAGTATGTTGATGACACAGAGGATTATATCAACATTATGCTTGATGACAAACAGAACCATCTTCTCCAAATGGGAGTCATGTTGACCACGGCAACTCTGGTGGTAAGTGCCTTCGTTGTTGTCGCTGGTATTTTTGGCATGAATATTAAAATTGAACTGTTTGATGATAAATTGTATGGGATGAGAGAGTTTATGTGGACTGTTGGTGGCGGCACTGCTGGGACCATATTCTTGTATGTGGTTGCTATTGCCTGGTGCAAGCACAAACGCTTGCTGGAGTGA
Protein sequence

>MsG0580024068.01.T01

MRGNVTGNANGSDDRWGTAAAAVPAIRKKGTSVRQWLVVDGTGEAQVIEAGKHAIMRRTGLPARDLRILDPLLSYPSTVLGRERAIVINLEHIKAIITANEVLLLNSRDPSVTPFVQELQARILRHHEATTTPRPDNQEDSHGGIKILPFEFVALEACLEAACSVLESEAKTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEHLLDDDEDMAEMYLTEKSLQQQLEQTSDEDTVETPDHDVHDNGVDVNQTGPGAEISLEAGLGGGEVNYEEDEDNLNSREQMFGASNLRDSRGTRASTTYSVTTTKLDVEELEMLLEAYFVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIKIELFDDKLYGMREFMWTVGGGTAGTIFLYVVAIAWCKHKRLLE*