AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024247.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024247.01.T01 MTR_1g114640 83.957 187 30 0 1 187 1 187 2.05e-112 320
MsG0580024247.01.T01 MTR_1g114640 81.818 165 30 0 1 165 1 165 1.03e-95 276
MsG0580024247.01.T01 MTR_1g033750 59.804 204 79 2 5 205 4 207 1.37e-76 229
MsG0580024247.01.T01 MTR_1g114690 50.794 189 86 3 1 187 1 184 8.32e-55 174
MsG0580024247.01.T01 MTR_1g114690 49.198 187 92 2 1 187 1 184 3.63e-54 172
MsG0580024247.01.T01 MTR_1g114750 47.619 189 95 2 1 187 1 187 3.36e-53 170
MsG0580024247.01.T01 MTR_1g114690 43.498 223 85 4 1 187 1 218 6.45e-48 157
MsG0580024247.01.T01 MTR_6g077990 53.793 145 59 2 65 205 43 183 2.07e-46 152
MsG0580024247.01.T01 MTR_6g077990 53.793 145 59 2 65 205 42 182 2.17e-46 152
MsG0580024247.01.T01 MTR_1g114690 50.000 168 77 3 1 166 1 163 6.53e-46 151
MsG0580024247.01.T01 MTR_1g115130 42.927 205 90 3 1 205 1 178 1.36e-43 145
MsG0580024247.01.T01 MTR_8g100065 50.993 151 65 3 60 205 23 169 4.86e-43 143
MsG0580024247.01.T01 MTR_6g077990 54.015 137 55 2 73 205 8 140 1.62e-42 140
MsG0580024247.01.T01 MTR_8g100065 50.993 151 65 3 60 205 45 191 1.72e-42 142
MsG0580024247.01.T01 MTR_6g077990 58.095 105 44 0 65 169 42 146 4.97e-38 129
MsG0580024247.01.T01 MTR_8g100065 57.009 107 45 1 60 165 23 129 2.19e-36 124
MsG0580024247.01.T01 MTR_8g100065 57.009 107 45 1 60 165 45 151 5.77e-36 124
MsG0580024247.01.T01 MTR_5g015750 80.556 72 14 0 1 72 1 72 6.88e-35 120
MsG0580024247.01.T01 MTR_1g114680 85.714 77 11 0 111 187 53 129 3.27e-31 112
MsG0580024247.01.T01 MTR_7g083570 32.215 149 101 0 28 176 84 232 1.20e-24 98.2
MsG0580024247.01.T01 MTR_3g100750 35.766 137 81 3 71 202 109 243 2.81e-23 94.0
MsG0580024247.01.T01 MTR_8g069110 31.176 170 98 4 50 205 90 254 3.26e-22 91.7
MsG0580024247.01.T01 MTR_2g076350 41.176 102 59 1 46 146 52 153 7.97e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024247.01.T01 AT5G08350 52.459 183 76 3 28 205 23 199 2.88e-55 175
MsG0580024247.01.T01 AT5G23370 48.293 205 101 1 1 205 1 200 4.15e-55 175
MsG0580024247.01.T01 AT5G23350 56.364 165 62 2 28 187 23 182 4.55e-54 172
MsG0580024247.01.T01 AT5G23360 47.525 202 87 4 1 197 1 188 3.47e-52 167
MsG0580024247.01.T01 AT4G01600 36.905 168 98 2 46 205 5 172 8.15e-30 109
MsG0580024247.01.T01 AT4G01600 34.343 198 121 3 16 205 15 211 1.58e-29 109
MsG0580024247.01.T01 AT4G01600 34.694 196 118 4 16 205 15 206 2.01e-26 101
MsG0580024247.01.T01 AT5G13200 31.373 153 105 0 35 187 77 229 6.21e-24 96.3
MsG0580024247.01.T01 AT1G28200 35.664 143 85 3 60 197 97 237 1.20e-22 92.4
MsG0580024247.01.T01 AT1G28200 35.664 143 85 3 60 197 97 237 1.20e-22 92.4
MsG0580024247.01.T01 AT5G13200 32.576 132 89 0 35 166 77 208 2.12e-22 90.9
MsG0580024247.01.T01 AT2G22475 32.895 152 91 4 59 205 133 278 6.27e-21 88.6
MsG0580024247.01.T01 AT2G22475 39.241 79 48 0 71 149 145 223 5.16e-16 74.3

Find 39 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTCACAAAGTTGCTTTC+TGG 0.165974 5:+2518160 MsG0580024247.01.T01:CDS
TTCCTTCATGAGCTACTTAA+TGG 0.213634 5:+2517299 MsG0580024247.01.T01:CDS
GCGTAGATGGTTTCGACTTC+TGG 0.218018 5:+2518416 MsG0580024247.01.T01:CDS
AAGATAACAGATACAGTAAA+AGG 0.228633 5:+2517982 MsG0580024247.01.T01:CDS
TTTACTGTATCTGTTATCTT+TGG 0.247894 5:-2517979 None:intergenic
ACTCTAATCAATTCTCCTTT+TGG 0.287784 5:-2518210 None:intergenic
AGATACTTGGCCGATTCTTC+TGG 0.307658 5:+2517365 MsG0580024247.01.T01:CDS
ATACAGTAAAAGGGAAATTA+AGG 0.314014 5:+2517992 MsG0580024247.01.T01:CDS
CTGACTAAGATGAACATGTT+TGG 0.338683 5:+2517728 MsG0580024247.01.T01:CDS
TGTTACTTATCAACCACATT+AGG 0.368586 5:+2518111 MsG0580024247.01.T01:CDS
TGACTAAGATGAACATGTTT+GGG 0.379362 5:+2517729 MsG0580024247.01.T01:CDS
GATGAACATGTTTGGGAGAA+AGG 0.393124 5:+2517736 MsG0580024247.01.T01:CDS
ATTGATTAGAGTTCTCTATA+AGG 0.399862 5:+2518221 MsG0580024247.01.T01:CDS
AGTAAAAGGGAAATTAAGGT+TGG 0.443877 5:+2517996 MsG0580024247.01.T01:CDS
GTTGTATATGCCAGAAGAAT+CGG 0.444156 5:-2517375 None:intergenic
GGAGGCAAAATTATTCAAGT+TGG 0.454364 5:+2518018 MsG0580024247.01.T01:CDS
ATGAACATGTTTGGGAGAAA+GGG 0.454867 5:+2517737 MsG0580024247.01.T01:CDS
GGCAAAATTATTCAAGTTGG+AGG 0.471062 5:+2518021 MsG0580024247.01.T01:CDS
ATCACCTCAACTCATTCAGT+TGG 0.473136 5:+2517329 MsG0580024247.01.T01:CDS
ATAGAAATAGTCAGCGTAGA+TGG 0.474979 5:+2518403 MsG0580024247.01.T01:CDS
ATGAAGAGGAGGCCAGCTAT+AGG 0.476519 5:-2518135 None:intergenic
CTGTAACCTGCAGTGAGACT+TGG 0.489945 5:+2517955 MsG0580024247.01.T01:intron
ATCAAGATCTCGTCACCAAA+AGG 0.523407 5:+2518195 MsG0580024247.01.T01:CDS
GTAAAAGGGAAATTAAGGTT+GGG 0.528971 5:+2517997 MsG0580024247.01.T01:CDS
TCACCTCAACTCATTCAGTT+GGG 0.537734 5:+2517330 MsG0580024247.01.T01:CDS
AGATAACAGATACAGTAAAA+GGG 0.540258 5:+2517983 MsG0580024247.01.T01:CDS
GTGTATGCAGTCCGAGAGCA+TGG 0.564348 5:+2517770 MsG0580024247.01.T01:CDS
CTGATTACATACCATGCTCT+CGG 0.564630 5:-2517781 None:intergenic
ATGTTTGGGAGAAAGGGTGA+TGG 0.565214 5:+2517743 MsG0580024247.01.T01:CDS
TCCATTACAAAATCAAAGCA+AGG 0.583726 5:+2517401 MsG0580024247.01.T01:CDS
TTCCATTAAGTAGCTCATGA+AGG 0.584791 5:-2517301 None:intergenic
GAAGTCATCAAAGAGATACT+TGG 0.585940 5:+2517352 MsG0580024247.01.T01:CDS
ACCACATTAGGTCCTATAGC+TGG 0.588201 5:+2518123 MsG0580024247.01.T01:CDS
CCAGAAAGCAACTTTGTGAG+TGG 0.608051 5:-2518160 None:intergenic
CTTTGTGAGTGGATATGAAG+AGG 0.608902 5:-2518149 None:intergenic
AAAGGGAAATTAAGGTTGGG+AGG 0.624997 5:+2518000 MsG0580024247.01.T01:CDS
GCCAGCTATAGGACCTAATG+TGG 0.654715 5:-2518124 None:intergenic
TGTGAGTGGATATGAAGAGG+AGG 0.670894 5:-2518146 None:intergenic
CTTCCCAACTGAATGAGTTG+AGG 0.692685 5:-2517333 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATAATTTAATTCATCA+TGG + Chr5:2517670-2517689 MsG0580024247.01.T01:intron 10.0%
!! TTGTTGCAACAAAAAAAAAA+TGG - Chr5:2517568-2517587 None:intergenic 20.0%
!!! AAATACAGTTTTGTTAATGT+AGG - Chr5:2517437-2517456 None:intergenic 20.0%
!!! AATGAAGTTTTAACTGATAA+TGG + Chr5:2517919-2517938 MsG0580024247.01.T01:intron 20.0%
!!! ATGGTCATTTTTATATTTTC+AGG + Chr5:2517689-2517708 MsG0580024247.01.T01:intron 20.0%
!!! CTTAATTTGTTTACTATTTC+AGG + Chr5:2518308-2518327 MsG0580024247.01.T01:intron 20.0%
! AAGATAACAGATACAGTAAA+AGG + Chr5:2517982-2518001 MsG0580024247.01.T01:CDS 25.0%
! AAGTGTGAAGAAAAGATAAA+AGG - Chr5:2518291-2518310 None:intergenic 25.0%
! AGATAACAGATACAGTAAAA+GGG + Chr5:2517983-2518002 MsG0580024247.01.T01:CDS 25.0%
! ATACAGTAAAAGGGAAATTA+AGG + Chr5:2517992-2518011 MsG0580024247.01.T01:CDS 25.0%
! ATCAATGAAAAATTGAAGGT+TGG - Chr5:2517820-2517839 None:intergenic 25.0%
! ATTGATTAGAGTTCTCTATA+AGG + Chr5:2518221-2518240 MsG0580024247.01.T01:CDS 25.0%
! TGGAATCAATGAAAAATTGA+AGG - Chr5:2517824-2517843 None:intergenic 25.0%
! TTTACTGTATCTGTTATCTT+TGG - Chr5:2517982-2518001 None:intergenic 25.0%
!! TATACTTTGAGTTTGAACTT+TGG + Chr5:2517880-2517899 MsG0580024247.01.T01:intron 25.0%
!!! TTTCTATGTACTTTTTCTGA+AGG - Chr5:2518390-2518409 None:intergenic 25.0%
AAACTACTATACAATGCTCT+TGG - Chr5:2517844-2517863 None:intergenic 30.0%
AAGTATACATCATAGCAGTT+TGG - Chr5:2517867-2517886 None:intergenic 30.0%
AGTAAAAGGGAAATTAAGGT+TGG + Chr5:2517996-2518015 MsG0580024247.01.T01:CDS 30.0%
CAATCAAAATAACTGATGTC+CGG + Chr5:2517522-2517541 MsG0580024247.01.T01:intron 30.0%
GTAAAAGGGAAATTAAGGTT+GGG + Chr5:2517997-2518016 MsG0580024247.01.T01:CDS 30.0%
TACAAAATCAAAGCAAGGTA+TGG + Chr5:2517406-2517425 MsG0580024247.01.T01:intron 30.0%
TCCATTACAAAATCAAAGCA+AGG + Chr5:2517401-2517420 MsG0580024247.01.T01:CDS 30.0%
TGACTAAGATGAACATGTTT+GGG + Chr5:2517729-2517748 MsG0580024247.01.T01:CDS 30.0%
! ACTCTAATCAATTCTCCTTT+TGG - Chr5:2518213-2518232 None:intergenic 30.0%
! TGTTACTTATCAACCACATT+AGG + Chr5:2518111-2518130 MsG0580024247.01.T01:CDS 30.0%
!! ACCTTGCTTTGATTTTGTAA+TGG - Chr5:2517405-2517424 None:intergenic 30.0%
AACTTCCCAACAAAATGTCT+CGG - Chr5:2517614-2517633 None:intergenic 35.0%
ATAGAAATAGTCAGCGTAGA+TGG + Chr5:2518403-2518422 MsG0580024247.01.T01:CDS 35.0%
ATGAACATGTTTGGGAGAAA+GGG + Chr5:2517737-2517756 MsG0580024247.01.T01:CDS 35.0%
CTGACTAAGATGAACATGTT+TGG + Chr5:2517728-2517747 MsG0580024247.01.T01:CDS 35.0%
GAAGTCATCAAAGAGATACT+TGG + Chr5:2517352-2517371 MsG0580024247.01.T01:CDS 35.0%
GGAGGCAAAATTATTCAAGT+TGG + Chr5:2518018-2518037 MsG0580024247.01.T01:CDS 35.0%
GTTGTATATGCCAGAAGAAT+CGG - Chr5:2517378-2517397 None:intergenic 35.0%
TTCCATTAAGTAGCTCATGA+AGG - Chr5:2517304-2517323 None:intergenic 35.0%
TTCCTTCATGAGCTACTTAA+TGG + Chr5:2517299-2517318 MsG0580024247.01.T01:CDS 35.0%
! ACATGCTGTATCTTTTCATG+TGG - Chr5:2518342-2518361 None:intergenic 35.0%
! TTCATTCGGGTTAAATAGCA+TGG - Chr5:2517468-2517487 None:intergenic 35.0%
!! AAGGAATGAGAGCATTTACA+TGG - Chr5:2518272-2518291 None:intergenic 35.0%
!! GGCAAAATTATTCAAGTTGG+AGG + Chr5:2518021-2518040 MsG0580024247.01.T01:CDS 35.0%
!!! CGGACATCAGTTATTTTGAT+TGG - Chr5:2517524-2517543 None:intergenic 35.0%
!!! GCTTCTTCACATTTTGACTT+TGG - Chr5:2518368-2518387 None:intergenic 35.0%
AAAGGGAAATTAAGGTTGGG+AGG + Chr5:2518000-2518019 MsG0580024247.01.T01:CDS 40.0%
ACTTCCCAACAAAATGTCTC+GGG - Chr5:2517613-2517632 None:intergenic 40.0%
AGTTTGAACTTTGGCGTGAT+TGG + Chr5:2517889-2517908 MsG0580024247.01.T01:intron 40.0%
ATCAAGATCTCGTCACCAAA+AGG + Chr5:2518195-2518214 MsG0580024247.01.T01:CDS 40.0%
ATCACCTCAACTCATTCAGT+TGG + Chr5:2517329-2517348 MsG0580024247.01.T01:CDS 40.0%
CTGATTACATACCATGCTCT+CGG - Chr5:2517784-2517803 None:intergenic 40.0%
CTTTGTGAGTGGATATGAAG+AGG - Chr5:2518152-2518171 None:intergenic 40.0%
GATGAACATGTTTGGGAGAA+AGG + Chr5:2517736-2517755 MsG0580024247.01.T01:CDS 40.0%
TAACTGATGTCCGGAATTCA+CGG + Chr5:2517531-2517550 MsG0580024247.01.T01:intron 40.0%
TCACCTCAACTCATTCAGTT+GGG + Chr5:2517330-2517349 MsG0580024247.01.T01:CDS 40.0%
! AACCTCGGGATGTTTTCATT+CGG - Chr5:2517482-2517501 None:intergenic 40.0%
! AACTTTTCAGCGTACAAGAC+GGG + Chr5:2518064-2518083 MsG0580024247.01.T01:CDS 40.0%
! ATGTCCCGAGACATTTTGTT+GGG + Chr5:2517606-2517625 MsG0580024247.01.T01:intron 40.0%
! CAACTTTTCAGCGTACAAGA+CGG + Chr5:2518063-2518082 MsG0580024247.01.T01:CDS 40.0%
! TATGTCCCGAGACATTTTGT+TGG + Chr5:2517605-2517624 MsG0580024247.01.T01:intron 40.0%
!!! GGTTTCGACTTCTGGTTTTT+GGG + Chr5:2518424-2518443 MsG0580024247.01.T01:CDS 40.0%
!!! TGGTTTCGACTTCTGGTTTT+TGG + Chr5:2518423-2518442 MsG0580024247.01.T01:CDS 40.0%
!!! TTTTGGTGACGAGATCTTGA+TGG - Chr5:2518196-2518215 None:intergenic 40.0%
ACCACATTAGGTCCTATAGC+TGG + Chr5:2518123-2518142 MsG0580024247.01.T01:CDS 45.0%
ATGTTTGGGAGAAAGGGTGA+TGG + Chr5:2517743-2517762 MsG0580024247.01.T01:CDS 45.0%
CCAGAAAGCAACTTTGTGAG+TGG - Chr5:2518163-2518182 None:intergenic 45.0%
CTTCCCAACTGAATGAGTTG+AGG - Chr5:2517336-2517355 None:intergenic 45.0%
TGTGAGTGGATATGAAGAGG+AGG - Chr5:2518149-2518168 None:intergenic 45.0%
! ACCTCGGGATGTTTTCATTC+GGG - Chr5:2517481-2517500 None:intergenic 45.0%
! ACTTTTCAGCGTACAAGACG+GGG + Chr5:2518065-2518084 MsG0580024247.01.T01:CDS 45.0%
! AGATACTTGGCCGATTCTTC+TGG + Chr5:2517365-2517384 MsG0580024247.01.T01:CDS 45.0%
! CCACTCACAAAGTTGCTTTC+TGG + Chr5:2518160-2518179 MsG0580024247.01.T01:CDS 45.0%
!! TGATTGGCTGCTGCTTTGTT+CGG - Chr5:2517508-2517527 None:intergenic 45.0%
!! TGAATTAAATTATATTAAAT+TGG - Chr5:2517667-2517686 None:intergenic 5.0%
ACCACTACCTCCGTGAATTC+CGG - Chr5:2517544-2517563 None:intergenic 50.0%
ACCCGAATGAAAACATCCCG+AGG + Chr5:2517477-2517496 MsG0580024247.01.T01:intron 50.0%
ATGAAGAGGAGGCCAGCTAT+AGG - Chr5:2518138-2518157 None:intergenic 50.0%
CTGTAACCTGCAGTGAGACT+TGG + Chr5:2517955-2517974 MsG0580024247.01.T01:intron 50.0%
GCCAGCTATAGGACCTAATG+TGG - Chr5:2518127-2518146 None:intergenic 50.0%
GCGTAGATGGTTTCGACTTC+TGG + Chr5:2518416-2518435 MsG0580024247.01.T01:CDS 50.0%
GTCTCGGGACATATTCACGA+TGG - Chr5:2517598-2517617 None:intergenic 50.0%
TTTGGTCCAAGTCTCACTGC+AGG - Chr5:2517964-2517983 None:intergenic 50.0%
! TGCTTTGTTCGGGACAACCT+CGG - Chr5:2517497-2517516 None:intergenic 50.0%
!! GATTGGCTGCTGCTTTGTTC+GGG - Chr5:2517507-2517526 None:intergenic 50.0%
CTGATGTCCGGAATTCACGG+AGG + Chr5:2517534-2517553 MsG0580024247.01.T01:intron 55.0%
GTGTATGCAGTCCGAGAGCA+TGG + Chr5:2517770-2517789 MsG0580024247.01.T01:CDS 55.0%
TCCGGAATTCACGGAGGTAG+TGG + Chr5:2517540-2517559 MsG0580024247.01.T01:intron 55.0%
! GCTTTGTTCGGGACAACCTC+GGG - Chr5:2517496-2517515 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 2517287 2518455 2517287 ID=MsG0580024247.01;Name=MsG0580024247.01
Chr5 mRNA 2517287 2518455 2517287 ID=MsG0580024247.01.T01;Parent=MsG0580024247.01;Name=MsG0580024247.01.T01;_AED=0.20;_eAED=0.21;_QI=0|1|0.5|1|1|1|4|0|205
Chr5 exon 2517287 2517422 2517287 ID=MsG0580024247.01.T01:exon:1737;Parent=MsG0580024247.01.T01
Chr5 exon 2517711 2517791 2517711 ID=MsG0580024247.01.T01:exon:1738;Parent=MsG0580024247.01.T01
Chr5 exon 2517968 2518242 2517968 ID=MsG0580024247.01.T01:exon:1739;Parent=MsG0580024247.01.T01
Chr5 exon 2518330 2518455 2518330 ID=MsG0580024247.01.T01:exon:1740;Parent=MsG0580024247.01.T01
Chr5 CDS 2517287 2517422 2517287 ID=MsG0580024247.01.T01:cds;Parent=MsG0580024247.01.T01
Chr5 CDS 2517711 2517791 2517711 ID=MsG0580024247.01.T01:cds;Parent=MsG0580024247.01.T01
Chr5 CDS 2517968 2518242 2517968 ID=MsG0580024247.01.T01:cds;Parent=MsG0580024247.01.T01
Chr5 CDS 2518330 2518455 2518330 ID=MsG0580024247.01.T01:cds;Parent=MsG0580024247.01.T01
Gene Sequence

>MsG0580024247.01.T01

ATGAAGACGTCATTCCTTCATGAGCTACTTAATGGAATTTCTATCACCTCAACTCATTCAGTTGGGAAGTCATCAAAGAGATACTTGGCCGATTCTTCTGGCATATACAACAAATCCATTACAAAATCAAAGCAAGGTAAATTTAATTCAGTTCTGACTAAGATGAACATGTTTGGGAGAAAGGGTGATGGTTTTGTGTATGCAGTCCGAGAGCATGTGAGACTTGGACCAAAGATAACAGATACAGTAAAAGGGAAATTAAGGTTGGGAGGCAAAATTATTCAAGTTGGAGGAATAGAGAAAGTGTTTATGCAACTTTTCAGCGTACAAGACGGGGAGAAGCTGTTGAAAGCGTCACAATGTTACTTATCAACCACATTAGGTCCTATAGCTGGCCTCCTCTTCATATCCACTCACAAAGTTGCTTTCTGGAGTGACAAATCCATCAAGATCTCGTCACCAAAAGGAGAATTGATTAGAGTTCTCTATAAGGTCTCTATTCCACATGAAAAGATACAGCATGTCAACCAAAGTCAAAATGTGAAGAAGCCTTCAGAAAAAGTACATAGAAATAGTCAGCGTAGATGGTTTCGACTTCTGGTTTTTGGGTTTCTTTAA

Protein sequence

>MsG0580024247.01.T01

MKTSFLHELLNGISITSTHSVGKSSKRYLADSSGIYNKSITKSKQGKFNSVLTKMNMFGRKGDGFVYAVREHVRLGPKITDTVKGKLRLGGKIIQVGGIEKVFMQLFSVQDGEKLLKASQCYLSTTLGPIAGLLFISTHKVAFWSDKSIKISSPKGELIRVLYKVSIPHEKIQHVNQSQNVKKPSEKVHRNSQRRWFRLLVFGFL*