AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024249.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024249.01.T01 MTR_1g114640 83.105 219 29 2 1 212 1 218 2.32e-131 369
MsG0580024249.01.T01 MTR_1g033750 63.636 220 71 3 5 215 4 223 7.66e-95 276
MsG0580024249.01.T01 MTR_1g114640 80.723 166 24 2 1 159 1 165 1.82e-90 263
MsG0580024249.01.T01 MTR_1g114690 53.070 228 84 3 1 215 1 218 3.37e-77 231
MsG0580024249.01.T01 MTR_1g114690 51.982 227 86 3 1 214 1 217 5.11e-75 226
MsG0580024249.01.T01 MTR_1g114750 48.214 224 104 2 1 215 1 221 1.46e-70 215
MsG0580024249.01.T01 MTR_1g114690 46.565 262 83 4 1 215 1 252 5.31e-70 214
MsG0580024249.01.T01 MTR_1g114680 93.103 116 8 0 105 220 53 168 2.33e-68 208
MsG0580024249.01.T01 MTR_8g100065 58.896 163 66 1 54 215 23 185 4.48e-63 194
MsG0580024249.01.T01 MTR_6g077990 55.625 160 71 0 58 217 42 201 1.08e-62 194
MsG0580024249.01.T01 MTR_6g077990 52.000 175 84 0 43 217 26 200 1.09e-62 194
MsG0580024249.01.T01 MTR_1g115130 60.000 150 60 0 67 216 46 195 1.29e-62 194
MsG0580024249.01.T01 MTR_8g100065 58.896 163 66 1 54 215 45 207 1.71e-62 194
MsG0580024249.01.T01 MTR_6g077990 55.629 151 67 0 67 217 8 158 1.61e-58 182
MsG0580024249.01.T01 MTR_1g114690 50.867 173 62 3 1 160 1 163 2.45e-48 157
MsG0580024249.01.T01 MTR_7g083570 35.052 194 126 0 20 213 82 275 4.52e-43 147
MsG0580024249.01.T01 MTR_3g100750 32.212 208 130 2 15 212 49 255 8.36e-42 142
MsG0580024249.01.T01 MTR_8g069110 35.359 181 106 2 42 212 88 267 4.81e-41 141
MsG0580024249.01.T01 MTR_6g077990 51.240 121 59 0 43 163 26 146 3.61e-39 132
MsG0580024249.01.T01 MTR_8g100065 59.813 107 42 1 54 159 23 129 1.26e-38 130
MsG0580024249.01.T01 MTR_8g100065 59.813 107 42 1 54 159 45 151 4.26e-38 130
MsG0580024249.01.T01 MTR_2g076350 33.491 212 134 3 6 214 20 227 1.60e-33 120
MsG0580024249.01.T01 MTR_5g015750 76.712 73 9 2 1 66 1 72 7.92e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024249.01.T01 AT5G23370 59.000 200 81 1 16 215 18 216 6.40e-80 238
MsG0580024249.01.T01 AT5G08350 57.000 200 74 3 21 215 23 215 3.94e-76 229
MsG0580024249.01.T01 AT5G23350 55.072 207 82 3 16 217 18 218 4.17e-75 226
MsG0580024249.01.T01 AT5G23360 59.756 164 66 0 54 217 47 210 1.06e-72 220
MsG0580024249.01.T01 AT4G01600 40.909 176 103 1 42 216 14 189 5.18e-48 156
MsG0580024249.01.T01 AT4G01600 40.909 176 103 1 42 216 53 228 1.65e-47 156
MsG0580024249.01.T01 AT5G13200 36.170 188 120 0 29 216 77 264 3.06e-44 149
MsG0580024249.01.T01 AT4G01600 43.709 151 84 1 67 216 73 223 5.27e-43 145
MsG0580024249.01.T01 AT1G28200 35.678 199 116 4 22 212 60 254 6.37e-42 143
MsG0580024249.01.T01 AT1G28200 35.678 199 116 4 22 212 60 254 6.37e-42 143
MsG0580024249.01.T01 AT2G22475 35.079 191 121 2 23 212 103 291 8.02e-42 144
MsG0580024249.01.T01 AT5G13200 33.333 132 88 0 29 160 77 208 2.16e-25 99.0
MsG0580024249.01.T01 AT4G40100 27.368 190 103 2 23 212 62 216 4.64e-22 90.5
MsG0580024249.01.T01 AT2G22475 31.757 148 98 2 23 169 103 248 6.80e-22 90.5

Find 42 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTTCGACTTCTGGTTTA+TGG 0.139805 5:+2532278 MsG0580024249.01.T01:CDS
TTTCTTCATGAGTTACTTAA+TGG 0.146739 5:+2531485 MsG0580024249.01.T01:CDS
CTATGGATGGTTTCGACTTC+TGG 0.198951 5:+2532271 MsG0580024249.01.T01:CDS
AAGATAACAGATACAGTAAA+AGG 0.228633 5:+2531925 MsG0580024249.01.T01:CDS
TTTACTGTATCTGTTATCTT+TGG 0.247894 5:-2531922 None:intergenic
GGTTTCGACTTCTGGTTTAT+GGG 0.291409 5:+2532279 MsG0580024249.01.T01:CDS
GTTGGGAGCCAGAATTCTTC+AGG 0.336328 5:+2531957 MsG0580024249.01.T01:CDS
CCCGCCAACCTGAAGAATTC+TGG 0.351542 5:-2531965 None:intergenic
GATGAACATGTTTGGAAGAA+AGG 0.373892 5:+2531704 MsG0580024249.01.T01:CDS
ATTCATTAGAGTTCACTATA+AGG 0.401080 5:+2532164 MsG0580024249.01.T01:CDS
TATCTGCCAGCTGATTCTTC+TGG 0.418036 5:+2531533 MsG0580024249.01.T01:CDS
ATGACTAAGATGAACATGTT+TGG 0.423883 5:+2531696 MsG0580024249.01.T01:CDS
ATGAACATGTTTGGAAGAAA+GGG 0.430526 5:+2531705 MsG0580024249.01.T01:CDS
ATACATAGAAATAGTCACTA+TGG 0.430727 5:+2532254 MsG0580024249.01.T01:CDS
CCAGAATTCTTCAGGTTGGC+GGG 0.441065 5:+2531965 MsG0580024249.01.T01:CDS
GCCAGAATTCTTCAGGTTGG+CGG 0.449134 5:+2531964 MsG0580024249.01.T01:CDS
CTCTAAATCTCAGAGGGAAA+AGG 0.451562 5:+2532347 MsG0580024249.01.T01:CDS
ATCAAAATTTCTTCCTCGAA+TGG 0.453468 5:+2532138 MsG0580024249.01.T01:CDS
ACCTTGCTTTGACTTTGTGA+TGG 0.474385 5:-2531570 None:intergenic
TCTAATGAATTCTCCATTCG+AGG 0.480167 5:-2532151 None:intergenic
ATGAAGAGAAGGCCAGCTAT+AGG 0.493362 5:-2532078 None:intergenic
AGCTTTAAGATATCTTCAGC+AGG 0.515990 5:+2532320 MsG0580024249.01.T01:CDS
AGTAAAAGGGAAACTGAGGT+TGG 0.524536 5:+2531939 MsG0580024249.01.T01:CDS
TTGTAACCTGCAGTGAGACT+TGG 0.527828 5:+2531898 MsG0580024249.01.T01:intron
ACCACGTCAGGTCCTATAGC+TGG 0.538413 5:+2532066 MsG0580024249.01.T01:CDS
AGATAACAGATACAGTAAAA+GGG 0.540258 5:+2531926 MsG0580024249.01.T01:CDS
GGAGCCAGAATTCTTCAGGT+TGG 0.551383 5:+2531961 MsG0580024249.01.T01:CDS
GGCTATCTCTAAATCTCAGA+GGG 0.564713 5:+2532341 MsG0580024249.01.T01:CDS
GCACATGGAGTCCGAGAGCA+TGG 0.566367 5:+2531738 MsG0580024249.01.T01:CDS
GTAAAAGGGAAACTGAGGTT+GGG 0.569074 5:+2531940 MsG0580024249.01.T01:CDS
TGTTACTTATCAACCACGTC+AGG 0.572611 5:+2532054 MsG0580024249.01.T01:CDS
ATAGAAATAGTCACTATGGA+TGG 0.592517 5:+2532258 MsG0580024249.01.T01:CDS
TCCATCACAAAGTCAAAGCA+AGG 0.594218 5:+2531569 MsG0580024249.01.T01:CDS
AAGATTACATACCATGCTCT+CGG 0.595383 5:-2531749 None:intergenic
ACAGAAAGCAACTTTGTGAG+TGG 0.605272 5:-2532103 None:intergenic
TGTGAGTGGAAATGAAGAGA+AGG 0.611200 5:-2532089 None:intergenic
CAGAATTCTTCAGGTTGGCG+GGG 0.646633 5:+2531966 MsG0580024249.01.T01:CDS
TGGCAGATATCTCTTTGATG+AGG 0.656890 5:-2531519 None:intergenic
ATACAGTAAAAGGGAAACTG+AGG 0.664591 5:+2531935 MsG0580024249.01.T01:CDS
TATATGCCAGAAGAATCAGC+TGG 0.671059 5:-2531539 None:intergenic
AGGCTATCTCTAAATCTCAG+AGG 0.678214 5:+2532340 MsG0580024249.01.T01:CDS
GCCAGCTATAGGACCTGACG+TGG 0.697419 5:-2532067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCTATGTATTTTTCTGA+TGG - Chr5:2532246-2532265 None:intergenic 20.0%
!!! TGAAATAATACAGTTTTGTT+AGG - Chr5:2531605-2531624 None:intergenic 20.0%
! AAGATAACAGATACAGTAAA+AGG + Chr5:2531925-2531944 MsG0580024249.01.T01:CDS 25.0%
! AGATAACAGATACAGTAAAA+GGG + Chr5:2531926-2531945 MsG0580024249.01.T01:CDS 25.0%
! ATACATAGAAATAGTCACTA+TGG + Chr5:2532254-2532273 MsG0580024249.01.T01:CDS 25.0%
! ATTCATTAGAGTTCACTATA+AGG + Chr5:2532164-2532183 MsG0580024249.01.T01:CDS 25.0%
! TAGAATGAAGTAGTAATTGA+TGG + Chr5:2531862-2531881 MsG0580024249.01.T01:intron 25.0%
! TTTACTGTATCTGTTATCTT+TGG - Chr5:2531925-2531944 None:intergenic 25.0%
! TTTCTTCATGAGTTACTTAA+TGG + Chr5:2531485-2531504 MsG0580024249.01.T01:CDS 25.0%
!! ATGATGCATCATACATATTT+TGG - Chr5:2531818-2531837 None:intergenic 25.0%
!! ATGATGCATCATTTGAAATT+TGG + Chr5:2531826-2531845 MsG0580024249.01.T01:intron 25.0%
!! CATTTGAAATTTGGAATGAT+TGG + Chr5:2531835-2531854 MsG0580024249.01.T01:intron 25.0%
!! TTTAATGTCATTGATGATCA+TGG + Chr5:2531639-2531658 MsG0580024249.01.T01:intron 25.0%
!!! ACTTGTTGTATTTTTTCATG+TGG - Chr5:2532198-2532217 None:intergenic 25.0%
!!! GTTTCATCATATTTTGACTT+TGG - Chr5:2532224-2532243 None:intergenic 25.0%
ATAGAAATAGTCACTATGGA+TGG + Chr5:2532258-2532277 MsG0580024249.01.T01:CDS 30.0%
ATCAAAATTTCTTCCTCGAA+TGG + Chr5:2532138-2532157 MsG0580024249.01.T01:CDS 30.0%
ATGAACATGTTTGGAAGAAA+GGG + Chr5:2531705-2531724 MsG0580024249.01.T01:CDS 30.0%
ATGACTAAGATGAACATGTT+TGG + Chr5:2531696-2531715 MsG0580024249.01.T01:CDS 30.0%
! ATTCGAGGAAGAAATTTTGA+TGG - Chr5:2532139-2532158 None:intergenic 30.0%
! CATGGTCATTTCATATTTTC+AGG + Chr5:2531657-2531676 MsG0580024249.01.T01:intron 30.0%
!! GATGTCTTCATTTTTGTTTG+AGG - Chr5:2531464-2531483 None:intergenic 30.0%
AAGATTACATACCATGCTCT+CGG - Chr5:2531752-2531771 None:intergenic 35.0%
ATACAGTAAAAGGGAAACTG+AGG + Chr5:2531935-2531954 MsG0580024249.01.T01:CDS 35.0%
GATGAACATGTTTGGAAGAA+AGG + Chr5:2531704-2531723 MsG0580024249.01.T01:CDS 35.0%
TCTAATGAATTCTCCATTCG+AGG - Chr5:2532154-2532173 None:intergenic 35.0%
!! AGCTTTAAGATATCTTCAGC+AGG + Chr5:2532320-2532339 MsG0580024249.01.T01:CDS 35.0%
ACAGAAAGCAACTTTGTGAG+TGG - Chr5:2532106-2532125 None:intergenic 40.0%
AGGCTATCTCTAAATCTCAG+AGG + Chr5:2532340-2532359 MsG0580024249.01.T01:CDS 40.0%
AGTAAAAGGGAAACTGAGGT+TGG + Chr5:2531939-2531958 MsG0580024249.01.T01:CDS 40.0%
CACAAAGTCAAAGCAAGGTA+TGG + Chr5:2531574-2531593 MsG0580024249.01.T01:intron 40.0%
CTCTAAATCTCAGAGGGAAA+AGG + Chr5:2532347-2532366 MsG0580024249.01.T01:CDS 40.0%
GGCTATCTCTAAATCTCAGA+GGG + Chr5:2532341-2532360 MsG0580024249.01.T01:CDS 40.0%
GTAAAAGGGAAACTGAGGTT+GGG + Chr5:2531940-2531959 MsG0580024249.01.T01:CDS 40.0%
TATATGCCAGAAGAATCAGC+TGG - Chr5:2531542-2531561 None:intergenic 40.0%
TCCATCACAAAGTCAAAGCA+AGG + Chr5:2531569-2531588 MsG0580024249.01.T01:CDS 40.0%
TGGCAGATATCTCTTTGATG+AGG - Chr5:2531522-2531541 None:intergenic 40.0%
TGTGAGTGGAAATGAAGAGA+AGG - Chr5:2532092-2532111 None:intergenic 40.0%
! ACCTTGCTTTGACTTTGTGA+TGG - Chr5:2531573-2531592 None:intergenic 40.0%
! GAGAGCATGGTATGTAATCT+TGG + Chr5:2531751-2531770 MsG0580024249.01.T01:intron 40.0%
! TGTTACTTATCAACCACGTC+AGG + Chr5:2532054-2532073 MsG0580024249.01.T01:CDS 40.0%
!! GGTTTCGACTTCTGGTTTAT+GGG + Chr5:2532279-2532298 MsG0580024249.01.T01:CDS 40.0%
!! TGGTTTCGACTTCTGGTTTA+TGG + Chr5:2532278-2532297 MsG0580024249.01.T01:CDS 40.0%
ATGAAGAGAAGGCCAGCTAT+AGG - Chr5:2532081-2532100 None:intergenic 45.0%
CTATGGATGGTTTCGACTTC+TGG + Chr5:2532271-2532290 MsG0580024249.01.T01:CDS 45.0%
TTGTAACCTGCAGTGAGACT+TGG + Chr5:2531898-2531917 MsG0580024249.01.T01:intron 45.0%
! AAGGGTGACAGTTTTGCACA+TGG + Chr5:2531723-2531742 MsG0580024249.01.T01:CDS 45.0%
! TATCTGCCAGCTGATTCTTC+TGG + Chr5:2531533-2531552 MsG0580024249.01.T01:CDS 45.0%
GGAGCCAGAATTCTTCAGGT+TGG + Chr5:2531961-2531980 MsG0580024249.01.T01:CDS 50.0%
GTTGGGAGCCAGAATTCTTC+AGG + Chr5:2531957-2531976 MsG0580024249.01.T01:CDS 50.0%
TTTGGTCCAAGTCTCACTGC+AGG - Chr5:2531907-2531926 None:intergenic 50.0%
!! CAGAATTCTTCAGGTTGGCG+GGG + Chr5:2531966-2531985 MsG0580024249.01.T01:CDS 50.0%
!! CCAGAATTCTTCAGGTTGGC+GGG + Chr5:2531965-2531984 MsG0580024249.01.T01:CDS 50.0%
!! GCCAGAATTCTTCAGGTTGG+CGG + Chr5:2531964-2531983 MsG0580024249.01.T01:CDS 50.0%
ACCACGTCAGGTCCTATAGC+TGG + Chr5:2532066-2532085 MsG0580024249.01.T01:CDS 55.0%
CCCGCCAACCTGAAGAATTC+TGG - Chr5:2531968-2531987 None:intergenic 55.0%
GCACATGGAGTCCGAGAGCA+TGG + Chr5:2531738-2531757 MsG0580024249.01.T01:CDS 60.0%
GCCAGCTATAGGACCTGACG+TGG - Chr5:2532070-2532089 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 2531457 2532374 2531457 ID=MsG0580024249.01;Name=MsG0580024249.01
Chr5 mRNA 2531457 2532374 2531457 ID=MsG0580024249.01.T01;Parent=MsG0580024249.01;Name=MsG0580024249.01.T01;_AED=0.41;_eAED=0.41;_QI=16|1|0.66|1|1|1|3|0|220
Chr5 exon 2531457 2531590 2531457 ID=MsG0580024249.01.T01:exon:1741;Parent=MsG0580024249.01.T01
Chr5 exon 2531679 2531759 2531679 ID=MsG0580024249.01.T01:exon:1742;Parent=MsG0580024249.01.T01
Chr5 exon 2531911 2532374 2531911 ID=MsG0580024249.01.T01:exon:1743;Parent=MsG0580024249.01.T01
Chr5 five_prime_UTR 2531457 2531472 2531457 ID=MsG0580024249.01.T01:five_prime_utr;Parent=MsG0580024249.01.T01
Chr5 CDS 2531473 2531590 2531473 ID=MsG0580024249.01.T01:cds;Parent=MsG0580024249.01.T01
Chr5 CDS 2531679 2531759 2531679 ID=MsG0580024249.01.T01:cds;Parent=MsG0580024249.01.T01
Chr5 CDS 2531911 2532374 2531911 ID=MsG0580024249.01.T01:cds;Parent=MsG0580024249.01.T01
Gene Sequence

>MsG0580024249.01.T01

ATGAAGACATCATTTCTTCATGAGTTACTTAATGGAATTTATGTTACCTCATCAAAGAGATATCTGCCAGCTGATTCTTCTGGCATATACAGCAAATCCATCACAAAGTCAAAGCAAGGTAAATTAAATTCAGTTATGACTAAGATGAACATGTTTGGAAGAAAGGGTGACAGTTTTGCACATGGAGTCCGAGAGCATGTGAGACTTGGACCAAAGATAACAGATACAGTAAAAGGGAAACTGAGGTTGGGAGCCAGAATTCTTCAGGTTGGCGGGGTAGAGAAAGTATTTATGAAACTTTTTAATGTAAACGACAGAGAGAAGCTGTTGAAAGCATCGCAGTGTTACTTATCAACCACGTCAGGTCCTATAGCTGGCCTTCTCTTCATTTCCACTCACAAAGTTGCTTTCTGTAGTGACAAATCCATCAAAATTTCTTCCTCGAATGGAGAATTCATTAGAGTTCACTATAAGGTCTCTATTCCACATGAAAAAATACAACAAGTCAACCAAAGTCAAAATATGATGAAACCATCAGAAAAATACATAGAAATAGTCACTATGGATGGTTTCGACTTCTGGTTTATGGGTTTCTTCAATTACAAGAAAGCTTTAAGATATCTTCAGCAGGCTATCTCTAAATCTCAGAGGGAAAAGGTGTAG

Protein sequence

>MsG0580024249.01.T01

MKTSFLHELLNGIYVTSSKRYLPADSSGIYSKSITKSKQGKLNSVMTKMNMFGRKGDSFAHGVREHVRLGPKITDTVKGKLRLGARILQVGGVEKVFMKLFNVNDREKLLKASQCYLSTTSGPIAGLLFISTHKVAFCSDKSIKISSSNGEFIRVHYKVSIPHEKIQQVNQSQNMMKPSEKYIEIVTMDGFDFWFMGFFNYKKALRYLQQAISKSQREKV*