Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024264.01.T01 | MQM04069.1 | 90.667 | 75 | 7 | 0 | 1 | 75 | 1 | 75 | 8.64E-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024264.01.T01 | Q9SMV6 | 83.562 | 73 | 12 | 0 | 1 | 73 | 1 | 73 | 9.50E-35 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024264.01.T01 | A0A843W469 | 90.667 | 75 | 7 | 0 | 1 | 75 | 1 | 75 | 4.13e-44 | 147 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010884.01 | MsG0580024264.01 | 0.856805 | 2.509818e-62 | 6.062089e-59 |
| MsG0480021845.01 | MsG0580024264.01 | 0.845665 | 3.524930e-59 | 5.917820e-56 |
| MsG0480022405.01 | MsG0580024264.01 | 0.807581 | 4.712502e-50 | 2.634404e-47 |
| MsG0480023973.01 | MsG0580024264.01 | 0.819497 | 1.125878e-52 | 8.678512e-50 |
| MsG0580024264.01 | MsG0780041470.01 | 0.826155 | 3.168644e-54 | 2.947703e-51 |
| MsG0580024264.01 | MsG0880046825.01 | 0.808408 | 3.143621e-50 | 1.796048e-47 |
| MsG0580024264.01 | MsG0880047179.01 | 0.813185 | 2.909317e-51 | 1.886718e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024264.01.T01 | MTR_5g007790 | 100.000 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 1.11e-44 | 153 |
| MsG0580024264.01.T01 | MTR_4g077660 | 91.781 | 73 | 6 | 0 | 1 | 73 | 1 | 73 | 2.69e-41 | 143 |
| MsG0580024264.01.T01 | MTR_8g013700 | 79.452 | 73 | 15 | 0 | 1 | 73 | 1 | 73 | 2.04e-33 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024264.01.T01 | AT5G17020 | 83.562 | 73 | 12 | 0 | 1 | 73 | 1 | 73 | 9.68e-36 | 127 |
| MsG0580024264.01.T01 | AT5G17020 | 83.562 | 73 | 12 | 0 | 1 | 73 | 1 | 73 | 1.04e-35 | 127 |
| MsG0580024264.01.T01 | AT3G03110 | 78.082 | 73 | 16 | 0 | 1 | 73 | 1 | 73 | 2.60e-33 | 120 |
Find 20 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCAAGTTCTTTGCTTTGC+AGG | 0.259869 | 5:+2729967 | MsG0580024264.01.T01:CDS |
| GCTACTGTTGCTGCCTTCTA+TGG | 0.318637 | 5:+2729170 | MsG0580024264.01.T01:CDS |
| TTTGCAATTCTCGCAAAATC+TGG | 0.354937 | 5:-2729886 | None:intergenic |
| CATTGCTTTCAGGGTGAAGT+AGG | 0.403203 | 5:-2730022 | None:intergenic |
| TTTAGATCCGGTGCCATAGA+AGG | 0.428767 | 5:-2729183 | None:intergenic |
| TTCTCCCTTCGCCATGGCCG+CGG | 0.437973 | 5:+2729100 | None:intergenic |
| ATAAACCTGTTCTTTAGATC+CGG | 0.476786 | 5:-2729195 | None:intergenic |
| TCGAGCAAAGGGACATCAAT+GGG | 0.493539 | 5:-2729146 | None:intergenic |
| TGGCACCGGATCTAAAGAAC+AGG | 0.514200 | 5:+2729190 | MsG0580024264.01.T01:CDS |
| CAACAGTAGCATCGAGCAAA+GGG | 0.527093 | 5:-2729157 | None:intergenic |
| ATGACTTGTAGCCACATGTC+TGG | 0.529906 | 5:-2729914 | None:intergenic |
| AACCTGCAAAGCAAAGAACT+TGG | 0.536982 | 5:-2729969 | None:intergenic |
| GTTGCTGCCTTCTATGGCAC+CGG | 0.537210 | 5:+2729176 | MsG0580024264.01.T01:CDS |
| ATCGAGCAAAGGGACATCAA+TGG | 0.541831 | 5:-2729147 | None:intergenic |
| ACGGAGTTTCTCCGCGGCCA+TGG | 0.542714 | 5:-2729111 | None:intergenic |
| GCAACAGTAGCATCGAGCAA+AGG | 0.558645 | 5:-2729158 | None:intergenic |
| ATTGCTTTCAGGGTGAAGTA+GGG | 0.568500 | 5:-2730021 | None:intergenic |
| CAATGGGTTGAGATAAATCA+CGG | 0.613732 | 5:-2729130 | None:intergenic |
| TGCAAAATAATCCAGACATG+TGG | 0.636102 | 5:+2729903 | MsG0580024264.01.T01:CDS |
| TAAATCACGGAGTTTCTCCG+CGG | 0.773462 | 5:-2729117 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCAAGTAATTAAATTTA+AGG | - | Chr5:2729812-2729831 | None:intergenic | 10.0% |
| !!! | ATGAAAAAAACATAATTTTG+AGG | + | Chr5:2729768-2729787 | MsG0580024264.01.T01:intron | 15.0% |
| !!! | TATTTAATTTTCTGCATATA+TGG | + | Chr5:2729506-2729525 | MsG0580024264.01.T01:intron | 15.0% |
| !! | AACCAAACAAAAAAACTTAA+CGG | - | Chr5:2729612-2729631 | None:intergenic | 20.0% |
| !!! | ATTCTTCATTTGATTTGTTT+TGG | + | Chr5:2729254-2729273 | MsG0580024264.01.T01:intron | 20.0% |
| !!! | TAAGGAAATTTTATGTGTTA+GGG | + | Chr5:2729345-2729364 | MsG0580024264.01.T01:intron | 20.0% |
| !!! | TAAGTTTTTTTGTTTGGTTT+CGG | + | Chr5:2729613-2729632 | MsG0580024264.01.T01:intron | 20.0% |
| !!! | TTAAGGAAATTTTATGTGTT+AGG | + | Chr5:2729344-2729363 | MsG0580024264.01.T01:intron | 20.0% |
| !!! | TTTTAACTGTGAATTTGTTT+TGG | + | Chr5:2729449-2729468 | MsG0580024264.01.T01:intron | 20.0% |
| ! | TTGAAAATGATGAAACGTTA+AGG | + | Chr5:2729327-2729346 | MsG0580024264.01.T01:intron | 25.0% |
| !! | AATTTTATGTGTTAGGGTTT+AGG | + | Chr5:2729351-2729370 | MsG0580024264.01.T01:intron | 25.0% |
| !! | ATTTTATGTGTTAGGGTTTA+GGG | + | Chr5:2729352-2729371 | MsG0580024264.01.T01:intron | 25.0% |
| !!! | CAACACTTTTTTAATTTGCA+TGG | - | Chr5:2729640-2729659 | None:intergenic | 25.0% |
| AAAAAACGCGCTATAATTGA+CGG | - | Chr5:2729575-2729594 | None:intergenic | 30.0% | |
| ACAAAAAAACTTAACGGCAA+AGG | - | Chr5:2729606-2729625 | None:intergenic | 30.0% | |
| ATAAACCTGTTCTTTAGATC+CGG | - | Chr5:2729198-2729217 | None:intergenic | 30.0% | |
| TGTGTTAATTAACGACTATG+AGG | + | Chr5:2729393-2729412 | MsG0580024264.01.T01:intron | 30.0% | |
| !!! | GCAACATTTTTTGAATTTGC+AGG | + | Chr5:2729735-2729754 | MsG0580024264.01.T01:intron | 30.0% |
| !!! | TGCCGTTAAGTTTTTTTGTT+TGG | + | Chr5:2729607-2729626 | MsG0580024264.01.T01:intron | 30.0% |
| AAAAACGCGCTATAATTGAC+GGG | - | Chr5:2729574-2729593 | None:intergenic | 35.0% | |
| ACTATGAGGATTAAGCTCTA+AGG | + | Chr5:2729407-2729426 | MsG0580024264.01.T01:intron | 35.0% | |
| TGCAAAATAATCCAGACATG+TGG | + | Chr5:2729903-2729922 | MsG0580024264.01.T01:CDS | 35.0% | |
| TTTGCAATTCTCGCAAAATC+TGG | - | Chr5:2729889-2729908 | None:intergenic | 35.0% | |
| !! | CAATGGGTTGAGATAAATCA+CGG | - | Chr5:2729133-2729152 | None:intergenic | 35.0% |
| AACCTGCAAAGCAAAGAACT+TGG | - | Chr5:2729972-2729991 | None:intergenic | 40.0% | |
| ATCCCTGATGTGAGCTTATT+CGG | + | Chr5:2729666-2729685 | MsG0580024264.01.T01:intron | 40.0% | |
| CACAATAAAATCACGTCGCA+TGG | - | Chr5:2729692-2729711 | None:intergenic | 40.0% | |
| TACCAAGTTCTTTGCTTTGC+AGG | + | Chr5:2729967-2729986 | MsG0580024264.01.T01:CDS | 40.0% | |
| ATCGAGCAAAGGGACATCAA+TGG | - | Chr5:2729150-2729169 | None:intergenic | 45.0% | |
| GTGAAACGAAGAACGAGCAA+TGG | - | Chr5:2729223-2729242 | None:intergenic | 45.0% | |
| TAAATCACGGAGTTTCTCCG+CGG | - | Chr5:2729120-2729139 | None:intergenic | 45.0% | |
| TCGAGCAAAGGGACATCAAT+GGG | - | Chr5:2729149-2729168 | None:intergenic | 45.0% | |
| TTTGATCCTGACGTCATACG+TGG | - | Chr5:2729715-2729734 | None:intergenic | 45.0% | |
| ! | ATGACTTGTAGCCACATGTC+TGG | - | Chr5:2729917-2729936 | None:intergenic | 45.0% |
| ! | CAACAGTAGCATCGAGCAAA+GGG | - | Chr5:2729160-2729179 | None:intergenic | 45.0% |
| !! | TTTAGATCCGGTGCCATAGA+AGG | - | Chr5:2729186-2729205 | None:intergenic | 45.0% |
| ATTGTGCCACGTATGACGTC+AGG | + | Chr5:2729706-2729725 | MsG0580024264.01.T01:intron | 50.0% | |
| GCTACTGTTGCTGCCTTCTA+TGG | + | Chr5:2729170-2729189 | MsG0580024264.01.T01:CDS | 50.0% | |
| GGCCGAATAAGCTCACATCA+GGG | - | Chr5:2729671-2729690 | None:intergenic | 50.0% | |
| TGGCCGAATAAGCTCACATC+AGG | - | Chr5:2729672-2729691 | None:intergenic | 50.0% | |
| ! | GCAACAGTAGCATCGAGCAA+AGG | - | Chr5:2729161-2729180 | None:intergenic | 50.0% |
| ! | TGGCACCGGATCTAAAGAAC+AGG | + | Chr5:2729190-2729209 | MsG0580024264.01.T01:CDS | 50.0% |
| GTTGCTGCCTTCTATGGCAC+CGG | + | Chr5:2729176-2729195 | MsG0580024264.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 2729113 | 2730036 | 2729113 | ID=MsG0580024264.01;Name=MsG0580024264.01 |
| Chr5 | mRNA | 2729113 | 2730036 | 2729113 | ID=MsG0580024264.01.T01;Parent=MsG0580024264.01;Name=MsG0580024264.01.T01;_AED=0.49;_eAED=0.58;_QI=0|0|0|1|1|1|2|0|87 |
| Chr5 | exon | 2729113 | 2729211 | 2729113 | ID=MsG0580024264.01.T01:exon:1816;Parent=MsG0580024264.01.T01 |
| Chr5 | exon | 2729872 | 2730036 | 2729872 | ID=MsG0580024264.01.T01:exon:1817;Parent=MsG0580024264.01.T01 |
| Chr5 | CDS | 2729113 | 2729211 | 2729113 | ID=MsG0580024264.01.T01:cds;Parent=MsG0580024264.01.T01 |
| Chr5 | CDS | 2729872 | 2730036 | 2729872 | ID=MsG0580024264.01.T01:cds;Parent=MsG0580024264.01.T01 |
| Gene Sequence |
| Protein sequence |
