Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021845.01.T01 | MCI24577.1 | 92.754 | 69 | 4 | 1 | 1 | 68 | 1 | 69 | 3.25E-37 | 129 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021845.01.T01 | Q9SMV6 | 78.261 | 69 | 14 | 1 | 1 | 68 | 1 | 69 | 2.46E-28 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021845.01.T01 | A0A392QKZ3 | 92.754 | 69 | 4 | 1 | 1 | 68 | 1 | 69 | 1.55e-37 | 129 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000010.01 | MsG0480021845.01 | 0.830637 | 2.625164e-55 | 2.781995e-52 |
| MsG0180000544.01 | MsG0480021845.01 | 0.808895 | 2.473459e-50 | 1.431233e-47 |
| MsG0180001706.01 | MsG0480021845.01 | 0.805838 | 1.100051e-49 | 5.880158e-47 |
| MsG0180003246.01 | MsG0480021845.01 | 0.806114 | 9.625101e-50 | 5.181272e-47 |
| MsG0180005515.01 | MsG0480021845.01 | 0.816399 | 5.642592e-52 | 3.993971e-49 |
| MsG0180005676.01 | MsG0480021845.01 | 0.810064 | 1.388017e-50 | 8.285245e-48 |
| MsG0180006034.01 | MsG0480021845.01 | 0.803105 | 4.085113e-49 | 2.035285e-46 |
| MsG0280010884.01 | MsG0480021845.01 | 0.813787 | 2.144983e-51 | 1.414051e-48 |
| MsG0280011059.01 | MsG0480021845.01 | 0.821383 | 4.158293e-53 | 3.377712e-50 |
| MsG0380014401.01 | MsG0480021845.01 | 0.831938 | 1.256699e-55 | 1.384243e-52 |
| MsG0480019162.01 | MsG0480021845.01 | 0.806511 | 7.939736e-50 | 4.317475e-47 |
| MsG0480019163.01 | MsG0480021845.01 | 0.852263 | 5.176929e-61 | 1.075781e-57 |
| MsG0480021490.01 | MsG0480021845.01 | 0.863258 | 2.829877e-64 | 8.525794e-61 |
| MsG0480021776.01 | MsG0480021845.01 | 0.835186 | 1.942605e-56 | 2.357643e-53 |
| MsG0480021845.01 | MsG0480022956.01 | 0.848437 | 6.133206e-60 | 1.125547e-56 |
| MsG0480021845.01 | MsG0480023973.01 | 0.855684 | 5.348550e-62 | 1.245036e-58 |
| MsG0480021845.01 | MsG0580024264.01 | 0.845665 | 3.524930e-59 | 5.917820e-56 |
| MsG0480021845.01 | MsG0580025182.01 | 0.821563 | 3.778565e-53 | 3.084864e-50 |
| MsG0480021845.01 | MsG0780038309.01 | 0.809541 | 1.798107e-50 | 1.058628e-47 |
| MsG0480021845.01 | MsG0780040148.01 | 0.800420 | 1.453320e-48 | 6.764257e-46 |
| MsG0480021845.01 | MsG0780041470.01 | 0.830415 | 2.974355e-55 | 3.131589e-52 |
| MsG0480021845.01 | MsG0880045605.01 | 0.847160 | 1.378624e-59 | 2.428784e-56 |
| MsG0480021845.01 | MsG0880046384.01 | 0.820194 | 7.803436e-53 | 6.133128e-50 |
| MsG0480021845.01 | MsG0880046825.01 | 0.856327 | 3.466671e-62 | 8.243715e-59 |
| MsG0480021845.01 | MsG0880047179.01 | 0.820889 | 5.403554e-53 | 4.329606e-50 |
| MsG0480021845.01 | MsG0880047402.01 | 0.810331 | 1.215712e-50 | 7.307285e-48 |
| MsG0380015212.01 | MsG0480021845.01 | 0.851106 | 1.101585e-60 | 2.205647e-57 |
| MsG0380016191.01 | MsG0480021845.01 | 0.805220 | 1.482957e-49 | 7.801620e-47 |
| MsG0380016828.01 | MsG0480021845.01 | 0.830965 | 2.182178e-55 | 2.335014e-52 |
| MsG0380017263.01 | MsG0480021845.01 | 0.805336 | 1.402358e-49 | 7.399657e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021845.01.T01 | MTR_4g077660 | 97.101 | 69 | 1 | 1 | 1 | 68 | 1 | 69 | 8.38e-39 | 135 |
| MsG0480021845.01.T01 | MTR_5g007790 | 88.406 | 69 | 7 | 1 | 1 | 68 | 1 | 69 | 8.77e-36 | 127 |
| MsG0480021845.01.T01 | MTR_8g013700 | 69.444 | 72 | 21 | 1 | 1 | 71 | 1 | 72 | 2.70e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021845.01.T01 | AT5G17020 | 78.261 | 69 | 14 | 1 | 1 | 68 | 1 | 69 | 2.51e-29 | 108 |
| MsG0480021845.01.T01 | AT5G17020 | 78.261 | 69 | 14 | 1 | 1 | 68 | 1 | 69 | 2.68e-29 | 108 |
| MsG0480021845.01.T01 | AT3G03110 | 73.913 | 69 | 17 | 1 | 1 | 68 | 1 | 69 | 1.72e-27 | 103 |
Find 32 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAAAAGGAAACGTAAT+TGG | 0.297188 | 4:+65067276 | None:intergenic |
| TTCGGGAGAGAGAAGAGTTC+GGG | 0.301257 | 4:+65067108 | None:intergenic |
| CTTAGATCCAGTGCCGTAGA+AGG | 0.421045 | 4:+65066912 | None:intergenic |
| ATCTCCTCCGAGCGAGTCTA+GGG | 0.425074 | 4:-65067068 | MsG0480021845.01.T01:five_prime_UTR |
| TGAGTGAGTTTGAAAGAAGA+GGG | 0.429009 | 4:+65067230 | None:intergenic |
| TTTCGGGAGAGAGAAGAGTT+CGG | 0.431562 | 4:+65067107 | None:intergenic |
| ATTCACTCTACTCTCGCTCT+AGG | 0.440316 | 4:-65067030 | MsG0480021845.01.T01:five_prime_UTR |
| AATCTCCTCCGAGCGAGTCT+AGG | 0.461260 | 4:-65067069 | MsG0480021845.01.T01:five_prime_UTR |
| GCCACCGTCGCCGCCTTCTA+CGG | 0.469747 | 4:-65066925 | MsG0480021845.01.T01:CDS |
| TCACTCTTCTTCTTCTCCGA+TGG | 0.473964 | 4:-65067001 | MsG0480021845.01.T01:exon |
| GACGCTACTAAGCTATATAT+AGG | 0.486822 | 4:+65067313 | None:intergenic |
| TTCACTCTACTCTCGCTCTA+GGG | 0.501547 | 4:-65067029 | MsG0480021845.01.T01:five_prime_UTR |
| AGAATAATAGGTATTTATAG+AGG | 0.505983 | 4:+65067164 | None:intergenic |
| GTGAGTGAGTTTGAAAGAAG+AGG | 0.508087 | 4:+65067229 | None:intergenic |
| GTCGCCGCCTTCTACGGCAC+TGG | 0.520083 | 4:-65066919 | MsG0480021845.01.T01:CDS |
| CTGAGCTTCTCAGCGGCCAT+CGG | 0.521732 | 4:+65066985 | None:intergenic |
| AGTGAGTTTGAAAGAAGAGG+GGG | 0.528376 | 4:+65067232 | None:intergenic |
| ACTCGCACGGAATGAAGAAA+AGG | 0.528494 | 4:+65067264 | None:intergenic |
| GCCGTAGAAGGCGGCGACGG+TGG | 0.532551 | 4:+65066924 | None:intergenic |
| TCGAGTAACGGAACATCGAT+CGG | 0.565699 | 4:+65066949 | None:intergenic |
| GCGACGGTGGCGTCGAGTAA+CGG | 0.567435 | 4:+65066937 | None:intergenic |
| GAGTGAGTTTGAAAGAAGAG+GGG | 0.588426 | 4:+65067231 | None:intergenic |
| ACAAAACCCCTAGACTCGCT+CGG | 0.604424 | 4:+65067061 | None:intergenic |
| ACTTGCAAAACAATGATATG+TGG | 0.610340 | 4:-65066538 | MsG0480021845.01.T01:CDS |
| AGTGCCGTAGAAGGCGGCGA+CGG | 0.612587 | 4:+65066921 | None:intergenic |
| GTGAGTTTGAAAGAAGAGGG+GGG | 0.626996 | 4:+65067233 | None:intergenic |
| AGATCCAGTGCCGTAGAAGG+CGG | 0.642341 | 4:+65066915 | None:intergenic |
| CGGCACTGGATCTAAGCAAG+AGG | 0.649693 | 4:-65066905 | MsG0480021845.01.T01:intron |
| TCGGGAGAGAGAAGAGTTCG+GGG | 0.658338 | 4:+65067109 | None:intergenic |
| TCTCCTCCGAGCGAGTCTAG+GGG | 0.701809 | 4:-65067067 | MsG0480021845.01.T01:five_prime_UTR |
| AAACCCCTAGACTCGCTCGG+AGG | 0.750988 | 4:+65067064 | None:intergenic |
| CAAATCTCTGAGCTTCTCAG+CGG | 0.769266 | 4:+65066978 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTAGTTAAGAATAAT+AGG | + | Chr4:65066670-65066689 | None:intergenic | 10.0% |
| !! | AGAATAATAGGTATTTATAG+AGG | + | Chr4:65066658-65066677 | None:intergenic | 20.0% |
| !! | TCAATTATTATCAACAACAT+TGG | + | Chr4:65067107-65067126 | None:intergenic | 20.0% |
| ! | GGTAAAAATGAAGAGAAAAA+AGG | + | Chr4:65066637-65066656 | None:intergenic | 25.0% |
| !! | AATGAATTACACGCATTTTA+GGG | - | Chr4:65066985-65067004 | MsG0480021845.01.T01:CDS | 25.0% |
| ACTTGCAAAACAATGATATG+TGG | - | Chr4:65067281-65067300 | MsG0480021845.01.T01:five_prime_UTR | 30.0% | |
| TGAAGAAAAGGAAACGTAAT+TGG | + | Chr4:65066546-65066565 | None:intergenic | 30.0% | |
| ! | AGATAATGTGATGCTGTTTA+CGG | - | Chr4:65067167-65067186 | MsG0480021845.01.T01:five_prime_UTR | 30.0% |
| ! | CAATGAATTACACGCATTTT+AGG | - | Chr4:65066984-65067003 | MsG0480021845.01.T01:CDS | 30.0% |
| !!! | TTGCTGTTTTTTTGTGTGTT+TGG | - | Chr4:65067194-65067213 | MsG0480021845.01.T01:five_prime_UTR | 30.0% |
| GACGCTACTAAGCTATATAT+AGG | + | Chr4:65066509-65066528 | None:intergenic | 35.0% | |
| TGAGTGAGTTTGAAAGAAGA+GGG | + | Chr4:65066592-65066611 | None:intergenic | 35.0% | |
| ! | ATACCAAGTTTTTGCATTGC+AGG | - | Chr4:65067344-65067363 | MsG0480021845.01.T01:five_prime_UTR | 35.0% |
| ! | TTCTAGAGAGAGAGAGATTT+CGG | + | Chr4:65066732-65066751 | None:intergenic | 35.0% |
| AGTGAGTTTGAAAGAAGAGG+GGG | + | Chr4:65066590-65066609 | None:intergenic | 40.0% | |
| GAACCTGCAATGCAAAAACT+TGG | + | Chr4:65067350-65067369 | None:intergenic | 40.0% | |
| GAGTGAGTTTGAAAGAAGAG+GGG | + | Chr4:65066591-65066610 | None:intergenic | 40.0% | |
| GTGAGTGAGTTTGAAAGAAG+AGG | + | Chr4:65066593-65066612 | None:intergenic | 40.0% | |
| TCTAGAGAGAGAGAGATTTC+GGG | + | Chr4:65066731-65066750 | None:intergenic | 40.0% | |
| TTGGAAATCGTGTAGATTGC+AGG | + | Chr4:65067088-65067107 | None:intergenic | 40.0% | |
| ACTCGCACGGAATGAAGAAA+AGG | + | Chr4:65066558-65066577 | None:intergenic | 45.0% | |
| AGCGACATTGTTGAAGAGAG+AGG | + | Chr4:65066957-65066976 | None:intergenic | 45.0% | |
| ATTCACTCTACTCTCGCTCT+AGG | - | Chr4:65066789-65066808 | MsG0480021845.01.T01:intron | 45.0% | |
| CAAATCTCTGAGCTTCTCAG+CGG | + | Chr4:65066844-65066863 | None:intergenic | 45.0% | |
| GCGACATTGTTGAAGAGAGA+GGG | + | Chr4:65066956-65066975 | None:intergenic | 45.0% | |
| GTGAGTTTGAAAGAAGAGGG+GGG | + | Chr4:65066589-65066608 | None:intergenic | 45.0% | |
| TCACTCTTCTTCTTCTCCGA+TGG | - | Chr4:65066818-65066837 | MsG0480021845.01.T01:intron | 45.0% | |
| TTCACTCTACTCTCGCTCTA+GGG | - | Chr4:65066790-65066809 | MsG0480021845.01.T01:intron | 45.0% | |
| TTTCGGGAGAGAGAAGAGTT+CGG | + | Chr4:65066715-65066734 | None:intergenic | 45.0% | |
| ! | TCGAGTAACGGAACATCGAT+CGG | + | Chr4:65066873-65066892 | None:intergenic | 45.0% |
| !!! | GTGCTGCTGATTCGATTTTG+CGG | - | Chr4:65067257-65067276 | MsG0480021845.01.T01:five_prime_UTR | 45.0% |
| !!! | TGCTGCTGATTCGATTTTGC+GGG | - | Chr4:65067258-65067277 | MsG0480021845.01.T01:five_prime_UTR | 45.0% |
| ACAAAACCCCTAGACTCGCT+CGG | + | Chr4:65066761-65066780 | None:intergenic | 50.0% | |
| CTTAGATCCAGTGCCGTAGA+AGG | + | Chr4:65066910-65066929 | None:intergenic | 50.0% | |
| TTCGGGAGAGAGAAGAGTTC+GGG | + | Chr4:65066714-65066733 | None:intergenic | 50.0% | |
| AATCTCCTCCGAGCGAGTCT+AGG | - | Chr4:65066750-65066769 | MsG0480021845.01.T01:intron | 55.0% | |
| AGATCCAGTGCCGTAGAAGG+CGG | + | Chr4:65066907-65066926 | None:intergenic | 55.0% | |
| ATCTCCTCCGAGCGAGTCTA+GGG | - | Chr4:65066751-65066770 | MsG0480021845.01.T01:intron | 55.0% | |
| TCGGGAGAGAGAAGAGTTCG+GGG | + | Chr4:65066713-65066732 | None:intergenic | 55.0% | |
| ! | CGGCACTGGATCTAAGCAAG+AGG | - | Chr4:65066914-65066933 | MsG0480021845.01.T01:CDS | 55.0% |
| AAACCCCTAGACTCGCTCGG+AGG | + | Chr4:65066758-65066777 | None:intergenic | 60.0% | |
| CTGAGCTTCTCAGCGGCCAT+CGG | + | Chr4:65066837-65066856 | None:intergenic | 60.0% | |
| TCTCCTCCGAGCGAGTCTAG+GGG | - | Chr4:65066752-65066771 | MsG0480021845.01.T01:intron | 60.0% | |
| AGTGCCGTAGAAGGCGGCGA+CGG | + | Chr4:65066901-65066920 | None:intergenic | 65.0% | |
| ! | GCGACGGTGGCGTCGAGTAA+CGG | + | Chr4:65066885-65066904 | None:intergenic | 65.0% |
| GCCACCGTCGCCGCCTTCTA+CGG | - | Chr4:65066894-65066913 | MsG0480021845.01.T01:intron | 70.0% | |
| GTCGCCGCCTTCTACGGCAC+TGG | - | Chr4:65066900-65066919 | MsG0480021845.01.T01:intron | 70.0% | |
| GCCGTAGAAGGCGGCGACGG+TGG | + | Chr4:65066898-65066917 | None:intergenic | 75.0% | |
| GGGGGACGCTCGCACTCGCA+CGG | + | Chr4:65066571-65066590 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 65066457 | 65067384 | 65066457 | ID=MsG0480021845.01;Name=MsG0480021845.01 |
| Chr4 | mRNA | 65066457 | 65067384 | 65066457 | ID=MsG0480021845.01.T01;Parent=MsG0480021845.01;Name=MsG0480021845.01.T01;_AED=0.51;_eAED=0.51;_QI=360|1|0.66|1|0.5|0.33|3|0|76 |
| Chr4 | exon | 65066457 | 65066588 | 65066457 | ID=MsG0480021845.01.T01:exon:20583;Parent=MsG0480021845.01.T01 |
| Chr4 | exon | 65066906 | 65067091 | 65066906 | ID=MsG0480021845.01.T01:exon:20582;Parent=MsG0480021845.01.T01 |
| Chr4 | exon | 65067112 | 65067384 | 65067112 | ID=MsG0480021845.01.T01:exon:20581;Parent=MsG0480021845.01.T01 |
| Chr4 | five_prime_UTR | 65067112 | 65067384 | 65067112 | ID=MsG0480021845.01.T01:five_prime_utr;Parent=MsG0480021845.01.T01 |
| Chr4 | five_prime_UTR | 65067005 | 65067091 | 65067005 | ID=MsG0480021845.01.T01:five_prime_utr;Parent=MsG0480021845.01.T01 |
| Chr4 | CDS | 65066906 | 65067004 | 65066906 | ID=MsG0480021845.01.T01:cds;Parent=MsG0480021845.01.T01 |
| Chr4 | CDS | 65066457 | 65066588 | 65066457 | ID=MsG0480021845.01.T01:cds;Parent=MsG0480021845.01.T01 |
| Gene Sequence |
| Protein sequence |
