Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024464.01.T01 | XP_003611214.1 | 96.923 | 325 | 3 | 3 | 1 | 318 | 1 | 325 | 0 | 619 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0580024464.01 | 0.820783 | 5.715016e-53 | 4.565449e-50 |
| MsG0080048036.01 | MsG0580024464.01 | 0.840793 | 7.021876e-58 | 1.011307e-54 |
| MsG0080048059.01 | MsG0580024464.01 | -0.806578 | 7.685459e-50 | 4.186316e-47 |
| MsG0080048301.01 | MsG0580024464.01 | 0.806985 | 6.304954e-50 | 3.470600e-47 |
| MsG0080048917.01 | MsG0580024464.01 | -0.838651 | 2.534440e-57 | 3.416866e-54 |
| MsG0080048918.01 | MsG0580024464.01 | -0.802093 | 6.607745e-49 | 3.208449e-46 |
| MsG0080048929.01 | MsG0580024464.01 | -0.814372 | 1.593017e-51 | 1.067007e-48 |
| MsG0080049024.01 | MsG0580024464.01 | -0.808066 | 3.717817e-50 | 2.104999e-47 |
| MsG0080049038.01 | MsG0580024464.01 | -0.810171 | 1.316044e-50 | 7.877442e-48 |
| MsG0080049053.01 | MsG0580024464.01 | -0.823868 | 1.098593e-53 | 9.575406e-51 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024464.01.T01 | MTR_5g011520 | 96.923 | 325 | 3 | 3 | 1 | 318 | 1 | 325 | 0.0 | 619 |
| MsG0580024464.01.T01 | MTR_8g036060 | 59.036 | 332 | 83 | 12 | 1 | 311 | 1 | 300 | 2.52e-88 | 267 |
| MsG0580024464.01.T01 | MTR_5g091630 | 62.727 | 220 | 69 | 2 | 41 | 259 | 1 | 208 | 3.22e-78 | 239 |
| MsG0580024464.01.T01 | MTR_1g073860 | 63.402 | 194 | 62 | 2 | 91 | 275 | 87 | 280 | 1.07e-68 | 217 |
| MsG0580024464.01.T01 | MTR_1g079810 | 68.235 | 170 | 54 | 0 | 92 | 261 | 68 | 237 | 5.57e-68 | 215 |
| MsG0580024464.01.T01 | MTR_1g044155 | 73.885 | 157 | 41 | 0 | 91 | 247 | 119 | 275 | 4.51e-67 | 214 |
| MsG0580024464.01.T01 | MTR_7g080980 | 68.874 | 151 | 47 | 0 | 107 | 257 | 66 | 216 | 2.32e-65 | 207 |
| MsG0580024464.01.T01 | MTR_3g068035 | 52.679 | 224 | 76 | 3 | 108 | 311 | 89 | 302 | 2.53e-65 | 208 |
| MsG0580024464.01.T01 | MTR_4g098450 | 61.275 | 204 | 70 | 2 | 47 | 250 | 34 | 228 | 6.45e-65 | 206 |
| MsG0580024464.01.T01 | MTR_3g100470 | 43.277 | 238 | 103 | 5 | 35 | 268 | 31 | 240 | 3.34e-57 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024464.01.T01 | AT4G22810 | 56.069 | 346 | 102 | 11 | 1 | 318 | 1 | 324 | 5.69e-80 | 246 |
| MsG0580024464.01.T01 | AT4G12050 | 57.349 | 347 | 111 | 9 | 1 | 318 | 1 | 339 | 3.01e-75 | 234 |
| MsG0580024464.01.T01 | AT2G45430 | 56.667 | 330 | 118 | 10 | 1 | 318 | 1 | 317 | 9.09e-75 | 233 |
| MsG0580024464.01.T01 | AT2G42940 | 68.293 | 164 | 52 | 0 | 96 | 259 | 51 | 214 | 1.81e-71 | 222 |
| MsG0580024464.01.T01 | AT2G35270 | 69.872 | 156 | 47 | 0 | 96 | 251 | 76 | 231 | 6.80e-71 | 221 |
| MsG0580024464.01.T01 | AT3G60870 | 56.540 | 237 | 71 | 6 | 91 | 318 | 52 | 265 | 3.89e-70 | 219 |
| MsG0580024464.01.T01 | AT4G14465 | 55.365 | 233 | 88 | 5 | 91 | 318 | 60 | 281 | 8.01e-69 | 216 |
| MsG0580024464.01.T01 | AT1G76500 | 65.244 | 164 | 48 | 1 | 95 | 249 | 69 | 232 | 2.51e-63 | 202 |
| MsG0580024464.01.T01 | AT3G04570 | 69.697 | 165 | 42 | 1 | 91 | 247 | 73 | 237 | 4.94e-63 | 202 |
| MsG0580024464.01.T01 | AT4G17800 | 71.034 | 145 | 42 | 0 | 107 | 251 | 91 | 235 | 4.33e-62 | 199 |
Find 91 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGTGACACTAAGGCAAC+CGG | 0.494419 | 5:-5239217 | MsG0580024464.01.T01:CDS |
| AAGGTTCGAGATTCTTTCGC+TGG | 0.482991 | 5:-5239163 | MsG0580024464.01.T01:CDS |
| AAGTTAGAATCCGGAGGAGG+TGG | 0.540145 | 5:-5239465 | MsG0580024464.01.T01:CDS |
| AATCTTAATCCTAATCTTCT+CGG | 0.405395 | 5:-5238820 | MsG0580024464.01.T01:CDS |
| ACAACAGCTCCAGGAGAAGC+CGG | 0.562958 | 5:+5239198 | None:intergenic |
| ACAGATCAAGATGAACAAAG+CGG | 0.697061 | 5:-5239567 | MsG0580024464.01.T01:CDS |
| ACCTCCTCCGGATTCTAACT+TGG | 0.395394 | 5:+5239467 | None:intergenic |
| ACCTTGAAGTTGTTGAATAG+AGG | 0.394601 | 5:+5238948 | None:intergenic |
| AGAAGATTAGGATTAAGATT+AGG | 0.426727 | 5:+5238823 | None:intergenic |
| AGACCAAGAGGAAGACCAGC+TGG | 0.509576 | 5:-5239411 | MsG0580024464.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATTAGGATTAGAATTAGG+AGG | + | Chr5:5239641-5239660 | None:intergenic | 30.0% | |
| ATGGATCAAATAACATCACA+TGG | - | Chr5:5238771-5238790 | MsG0580024464.01.T01:CDS | 30.0% | |
| TGGTTTGACCATATATTTAG+CGG | - | Chr5:5239376-5239395 | MsG0580024464.01.T01:CDS | 30.0% | |
| ACAGATCAAGATGAACAAAG+CGG | - | Chr5:5238909-5238928 | MsG0580024464.01.T01:CDS | 35.0% | |
| ACCTTGAAGTTGTTGAATAG+AGG | + | Chr5:5239531-5239550 | None:intergenic | 35.0% | |
| AGATGCCTTCTGATAATTTC+TGG | - | Chr5:5239681-5239700 | MsG0580024464.01.T01:CDS | 35.0% | |
| CTGTCACGTGTGATAATAAT+CGG | + | Chr5:5239113-5239132 | None:intergenic | 35.0% | |
| GAAGCAGACATAATAACAAC+AGG | + | Chr5:5239458-5239477 | None:intergenic | 35.0% | |
| GGTTTGACCATATATTTAGC+GGG | - | Chr5:5239377-5239396 | MsG0580024464.01.T01:CDS | 35.0% | |
| TCCTCTATTCAACAACTTCA+AGG | - | Chr5:5239527-5239546 | MsG0580024464.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 5238771 | 5239727 | 5238771 | ID=MsG0580024464.01;Name=MsG0580024464.01 |
| Chr5 | mRNA | 5238771 | 5239727 | 5238771 | ID=MsG0580024464.01.T01;Parent=MsG0580024464.01;Name=MsG0580024464.01.T01;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|318 |
| Chr5 | exon | 5238771 | 5239727 | 5238771 | ID=MsG0580024464.01.T01:exon:755;Parent=MsG0580024464.01.T01 |
| Chr5 | CDS | 5238771 | 5239727 | 5238771 | ID=MsG0580024464.01.T01:cds;Parent=MsG0580024464.01.T01 |
| Gene Sequence |
>MsG0580024464.01.T01 ATGGATCAAATAACATCACATGGACATTCACTTCCTCCACCTTTCCACACAGCAAGAGATCTTCATCTTCACCATCAACACCAACAGCAGCAACAACAGCACCATCAATTCCACACTTTACAGCAGCAACAACAAACAACAGATCAAGATGAACAAAGCGGAAGTAGCAGCGGCGGTGGACTCAACCTCACAAACCGAGAAGAAAACAGCAACAACAAGTTCAGCACAGAATTTAGCCCCAAGTTAGAATCCGGAGGAGGTGGTTCCGGTGGCGACACGGATTCAATGACAAGAAGACCAAGAGGAAGACCAGCTGGATCCAAAAACAAACCAAAACCACCGATTATTATCACACGTGACAGCGCAAACGCCTTGAAAACTCATGTGATGGAGGTTGCAGACGGTTGCGACGTTGTTGAAAGTGTCAATAACTTTGCTAGACGCCGTCAAAGAGGCGTTTGCATCATGAGTGGGACAGGAACGGTTACAAACGTGACACTAAGGCAACCGGCTTCTCCTGGAGCTGTTGTTACACTTCATGGAAGGTTCGAGATTCTTTCGCTGGCTGGATCGTTTCTTCCACCACCTGCTCCGCCCGCTGCTTCTGGTTTGACCATATATTTAGCGGGCGGACAAGGGCAGGTTGTTGGTGGAAGTGTTGTTGGTGCTTTGATTGCTTCTGGACCTGTTGTTATTATGTCTGCTTCTTTTAGCAACGCTGCTTATGAGAGACTTCCTTTGGAAGATGATGATGGTTCCTCTATTCAACAACTTCAAGGTGGTGGTTCTCCAAGTGGTGGTGGTGGTGTTCAGCAACAACAACAGCTTTTAGGAGATTCAACTGCTCCACTTTTTCAAGCTATGCCTCCTCCTAATTCTAATCCTAATCTTAATCCTAATCTTCTCGGTCAGATGCCTTCTGATAATTTCTGGCCAACCGGTCGTTCTCCGTATTGA |
| Protein sequence |
>MsG0580024464.01.T01 MDQITSHGHSLPPPFHTARDLHLHHQHQQQQQQHHQFHTLQQQQQTTDQDEQSGSSSGGGLNLTNREENSNNKFSTEFSPKLESGGGGSGGDTDSMTRRPRGRPAGSKNKPKPPIIITRDSANALKTHVMEVADGCDVVESVNNFARRRQRGVCIMSGTGTVTNVTLRQPASPGAVVTLHGRFEILSLAGSFLPPPAPPAASGLTIYLAGGQGQVVGGSVVGALIASGPVVIMSASFSNAAYERLPLEDDDGSSIQQLQGGGSPSGGGGVQQQQQLLGDSTAPLFQAMPPPNSNPNLNPNLLGQMPSDNFWPTGRSPY* |
