Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024877.01.T01 | XP_003611862.1 | 95.993 | 574 | 23 | 0 | 1 | 574 | 90 | 663 | 0 | 1160 |
| MsG0580024877.01.T01 | XP_003611862.1 | 27.869 | 427 | 242 | 6 | 67 | 461 | 55 | 447 | 4.77E-37 | 157 |
| MsG0580024877.01.T01 | XP_003611862.1 | 25.325 | 308 | 197 | 4 | 166 | 473 | 53 | 327 | 2.68E-18 | 101 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003097.01 | MsG0580024877.01 | 0.806147 | 9.474241e-50 | 5.104307e-47 |
| MsG0180003188.01 | MsG0580024877.01 | 0.812972 | 3.238781e-51 | 2.088414e-48 |
| MsG0180004463.01 | MsG0580024877.01 | 0.802274 | 6.064713e-49 | 2.958406e-46 |
| MsG0280007164.01 | MsG0580024877.01 | 0.807938 | 3.958663e-50 | 2.233757e-47 |
| MsG0380016847.01 | MsG0580024877.01 | 0.808882 | 2.489857e-50 | 1.440231e-47 |
| MsG0380017327.01 | MsG0580024877.01 | 0.834953 | 2.224390e-56 | 2.680904e-53 |
| MsG0480018163.01 | MsG0580024877.01 | 0.817472 | 3.240117e-52 | 2.361969e-49 |
| MsG0580024165.01 | MsG0580024877.01 | 0.825400 | 4.784415e-54 | 4.356233e-51 |
| MsG0580024877.01 | MsG0780036477.01 | 0.807416 | 5.109599e-50 | 2.844360e-47 |
| MsG0580024877.01 | MsG0780040057.01 | 0.812950 | 3.276275e-51 | 2.111296e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024877.01.T01 | MTR_5g018690 | 95.993 | 574 | 23 | 0 | 1 | 574 | 90 | 663 | 0.0 | 1160 |
| MsG0580024877.01.T01 | MTR_5g018690 | 27.869 | 427 | 242 | 6 | 67 | 461 | 55 | 447 | 5.78e-41 | 157 |
| MsG0580024877.01.T01 | MTR_5g018690 | 25.325 | 308 | 197 | 4 | 166 | 473 | 53 | 327 | 3.25e-22 | 101 |
| MsG0580024877.01.T01 | MTR_7g093900 | 40.399 | 552 | 328 | 1 | 1 | 551 | 100 | 651 | 8.43e-149 | 444 |
| MsG0580024877.01.T01 | MTR_7g093900 | 27.512 | 418 | 234 | 7 | 94 | 477 | 92 | 474 | 2.02e-43 | 165 |
| MsG0580024877.01.T01 | MTR_7g093900 | 40.585 | 547 | 324 | 1 | 1 | 546 | 50 | 596 | 3.43e-145 | 440 |
| MsG0580024877.01.T01 | MTR_7g093900 | 27.512 | 418 | 234 | 7 | 94 | 477 | 42 | 424 | 5.80e-43 | 165 |
| MsG0580024877.01.T01 | MTR_4g024860 | 41.123 | 552 | 324 | 1 | 4 | 554 | 116 | 667 | 8.81e-142 | 436 |
| MsG0580024877.01.T01 | MTR_4g024860 | 27.139 | 409 | 233 | 5 | 101 | 477 | 112 | 487 | 6.00e-42 | 162 |
| MsG0580024877.01.T01 | MTR_2g036960 | 38.889 | 558 | 306 | 4 | 6 | 559 | 77 | 603 | 1.32e-136 | 413 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024877.01.T01 | AT2G29760 | 40.285 | 561 | 333 | 2 | 1 | 559 | 86 | 646 | 4.29e-152 | 454 |
| MsG0580024877.01.T01 | AT2G29760 | 27.778 | 396 | 220 | 7 | 99 | 461 | 83 | 445 | 2.09e-42 | 162 |
| MsG0580024877.01.T01 | AT2G22410 | 40.647 | 556 | 327 | 1 | 5 | 557 | 113 | 668 | 1.78e-151 | 451 |
| MsG0580024877.01.T01 | AT2G22410 | 26.764 | 411 | 234 | 5 | 101 | 477 | 108 | 485 | 5.72e-46 | 172 |
| MsG0580024877.01.T01 | AT2G22410 | 25.622 | 402 | 231 | 6 | 1 | 369 | 213 | 579 | 1.35e-40 | 157 |
| MsG0580024877.01.T01 | AT4G14820 | 36.607 | 560 | 354 | 1 | 1 | 559 | 66 | 625 | 4.59e-141 | 426 |
| MsG0580024877.01.T01 | AT3G15930 | 36.945 | 563 | 348 | 2 | 1 | 559 | 90 | 649 | 2.73e-135 | 410 |
| MsG0580024877.01.T01 | AT3G15930 | 26.316 | 456 | 258 | 9 | 63 | 476 | 41 | 460 | 7.02e-43 | 163 |
| MsG0580024877.01.T01 | AT2G13600 | 37.367 | 562 | 345 | 2 | 1 | 555 | 108 | 669 | 1.34e-132 | 403 |
| MsG0580024877.01.T01 | AT2G13600 | 26.556 | 482 | 250 | 9 | 67 | 478 | 42 | 489 | 1.35e-51 | 188 |
Find 125 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATGGTAATTTGGATT+TGG | 0.233157 | 5:+11123820 | MsG0580024877.01.T01:CDS |
| AAACAACAAAATTGCTTCTT+CGG | 0.351830 | 5:-11123729 | None:intergenic |
| AAACATGGTAATTTGGATTT+GGG | 0.335265 | 5:+11123821 | MsG0580024877.01.T01:CDS |
| AAAGGGATTGTAAACTGGTT+TGG | 0.405990 | 5:-11123476 | None:intergenic |
| AACAAGTATATACTCTATGC+TGG | 0.453754 | 5:+11124969 | MsG0580024877.01.T01:CDS |
| AACATGAATTTATTTGGTTG+AGG | 0.427239 | 5:-11123365 | None:intergenic |
| AAGATTTGTGCATCTTTATA+TGG | 0.254300 | 5:+11123844 | MsG0580024877.01.T01:CDS |
| AATGGATCATCTAAAGTCTG+CGG | 0.615203 | 5:+11124999 | MsG0580024877.01.T01:CDS |
| AATTGAATCAATTTCAATAC+CGG | 0.471248 | 5:-11123873 | None:intergenic |
| AATTGTTACAAATGCACTCA+TGG | 0.472145 | 5:+11123892 | MsG0580024877.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAGTATATACTCTATGC+TGG | + | Chr5:11124969-11124988 | MsG0580024877.01.T01:CDS | 30.0% | |
| AATTGTTACAAATGCACTCA+TGG | + | Chr5:11123892-11123911 | MsG0580024877.01.T01:CDS | 30.0% | |
| ACAAGTATATACTCTATGCT+GGG | + | Chr5:11124970-11124989 | MsG0580024877.01.T01:CDS | 30.0% | |
| ACATTATGAATTCGACTTTC+GGG | + | Chr5:11124557-11124576 | MsG0580024877.01.T01:CDS | 30.0% | |
| ATAGACATGTATGCAAAATG+CGG | + | Chr5:11124307-11124326 | MsG0580024877.01.T01:CDS | 30.0% | |
| ATCAGCTTCATTGAAATTGA+TGG | + | Chr5:11124901-11124920 | MsG0580024877.01.T01:CDS | 30.0% | |
| ATGTAAACATTTGGATGTAG+AGG | + | Chr5:11125029-11125048 | MsG0580024877.01.T01:CDS | 30.0% | |
| ATTAACGCTTATGCTAATCA+TGG | + | Chr5:11124004-11124023 | MsG0580024877.01.T01:CDS | 30.0% | |
| CATCCTATAGAAAAGATCAA+CGG | - | Chr5:11124137-11124156 | None:intergenic | 30.0% | |
| CATCTTTATATGGTCATTAC+CGG | + | Chr5:11123854-11123873 | MsG0580024877.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 11123350 | 11125074 | 11123350 | ID=MsG0580024877.01;Name=MsG0580024877.01 |
| Chr5 | mRNA | 11123350 | 11125074 | 11123350 | ID=MsG0580024877.01.T01;Parent=MsG0580024877.01;Name=MsG0580024877.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|574 |
| Chr5 | exon | 11123350 | 11125074 | 11123350 | ID=MsG0580024877.01.T01:exon:449;Parent=MsG0580024877.01.T01 |
| Chr5 | CDS | 11123350 | 11125074 | 11123350 | ID=MsG0580024877.01.T01:cds;Parent=MsG0580024877.01.T01 |
| Gene Sequence |
>MsG0580024877.01.T01 ATGTTTGATCAAATTCCTCAACCAAATAAATTCATGTTTAACCATTTAATTAAGGGTTATTCTAATTCCAATGACCCAGTTAAGTCTTTGTTGCTGTACCGTCGAATGGTGGGTGATGGTCTTTTACCAAACCAGTTTACAATCCCTTTTGTTCTGAAAGCTTGTGCTGCCAAATCGTGTTATTGGGTCGGTGTTTGTGTTCATGCTCAGTCTTTTAAGCTTGGAATGGGGTCACATGCTTGTGTTCAGAACGCAATTTTGAATGTTTATGTTGCTTGTGGTTTGATAACAAGTGCAAGGAGAGTGTTTGATGATATTTCTGAAAGGACCTTGGTCTCGTGGAATTCGATGATTAATGGGTATTCTAAAATGGGTTGGTCCGAAGAAGCAATTTTGTTGTTTCGAGAAATGCAAGAAGTTGGATTGGAGCCTGATGTGTTTACGTTGGTTGGCTTGCTTTCTGTTTCGACAAAACATGGTAATTTGGATTTGGGAAGATTTGTGCATCTTTATATGGTCATTACCGGTATTGAAATTGATTCAATTGTTACAAATGCACTCATGGATATGTATGCCAAGTGTGGGAATTTGAAATGTGCTAAAAGTGTTTTTGATCAAATGCTTGATAAGGATGTTGTTTCTTGGACGTGTATGATTAACGCTTATGCTAATCATGGACTTATTGATTGTGCTTTGGAATTTTTTAACCAGATGCCGGGGAAGAACGTGGTTTCTTGGAATTCAATAATTTGGTGTCATGTTCAAGAGGGACTTTATCCGGAAGCCGTTGATCTTTTCTATAGGATGTGTGATTCAGGCGTGATGCCTAATGATGCTACTCTTGTTGCCATTCTTTCATCCTGCAGTCACATGGGTGATCTAGCATTGGGAAAACAAGCCCACAGCTACATTTTTGATAACAACATTACACTGAGTGTGACGCTTTGCAACGCTATAATAGACATGTATGCAAAATGCGGTGCTCTTCAGATTGCCATGGACGTCTTCTTCGGGATGCCTGAGAAGAATGCAGTGTCATGGAATGTTATTATCGGGGCACTTGCTCTGCATGGTTATGGAAAAGAAGCTATTGAGATGTTTGAGAAGATGCAGGCAAGTGGAGTTTGTCCTGATGAGATTACTTTTACAGGTTTGCTTTCTGCTTGTAGTCACAGTGGTCTTGTGGACATGGGGCAATATTACTTTGACATTATGAATTCGACTTTCGGGATTTCTCCTGATGTTGAACATTATGCGTGCATGGTCGATCTTTTAGGTCGCCGTGGATTGTTAGGAGAAGCGATATCATTGATTAAGAAGATGCCTGTTAAACCAGATGTTGTTGTTTGGAGTGCTTTACTTGGTGCTTGTCGAACCTATGGGAATCTAGCAATTGGTAAGCAAATCATGAAGCAGTTGTTGGAGTTGGGACGATACAATTCTGGTCTTTATGTGCTTCTCTCAAATATGTATTCAGAATCTCAAAGATGGGAAGACATGAAAAATATTTGGAAAATACTGGATCAAAATGGGATAAAAAAGTGCAGGGCAATCAGCTTCATTGAAATTGATGGCTGTTGTTATCAATTTATGGTGGATGATAAAAGACATGGAGCTTCAACAAGTATATACTCTATGCTGGGTCAATTAATGGATCATCTAAAGTCTGCGGGGTATCCATGTAAACATTTGGATGTAGAGGAAATATACTGCTCTTATATTTAA |
| Protein sequence |
>MsG0580024877.01.T01 MFDQIPQPNKFMFNHLIKGYSNSNDPVKSLLLYRRMVGDGLLPNQFTIPFVLKACAAKSCYWVGVCVHAQSFKLGMGSHACVQNAILNVYVACGLITSARRVFDDISERTLVSWNSMINGYSKMGWSEEAILLFREMQEVGLEPDVFTLVGLLSVSTKHGNLDLGRFVHLYMVITGIEIDSIVTNALMDMYAKCGNLKCAKSVFDQMLDKDVVSWTCMINAYANHGLIDCALEFFNQMPGKNVVSWNSIIWCHVQEGLYPEAVDLFYRMCDSGVMPNDATLVAILSSCSHMGDLALGKQAHSYIFDNNITLSVTLCNAIIDMYAKCGALQIAMDVFFGMPEKNAVSWNVIIGALALHGYGKEAIEMFEKMQASGVCPDEITFTGLLSACSHSGLVDMGQYYFDIMNSTFGISPDVEHYACMVDLLGRRGLLGEAISLIKKMPVKPDVVVWSALLGACRTYGNLAIGKQIMKQLLELGRYNSGLYVLLSNMYSESQRWEDMKNIWKILDQNGIKKCRAISFIEIDGCCYQFMVDDKRHGASTSIYSMLGQLMDHLKSAGYPCKHLDVEEIYCSYI* |
