Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017327.01.T01 | MCH91981.1 | 68.482 | 257 | 81 | 0 | 4 | 260 | 2 | 258 | 5.69E-127 | 375 |
| MsG0380017327.01.T02 | MCH91981.1 | 73.659 | 205 | 54 | 0 | 1 | 205 | 54 | 258 | 7.72E-107 | 322 |
| MsG0380017327.01.T03 | MCH91981.1 | 77.041 | 196 | 44 | 1 | 1 | 196 | 64 | 258 | 1.37E-105 | 318 |
| MsG0380017327.01.T04 | MCH91981.1 | 77.005 | 187 | 43 | 0 | 9 | 195 | 72 | 258 | 3.26E-100 | 305 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017327.01.T01 | Q9SMZ2 | 54.4 | 250 | 114 | 0 | 4 | 253 | 617 | 866 | 2.91E-90 | 290 |
| MsG0380017327.01.T02 | Q9SMZ2 | 57.292 | 192 | 82 | 0 | 7 | 198 | 675 | 866 | 9.46E-73 | 240 |
| MsG0380017327.01.T03 | Q9SMZ2 | 58.919 | 185 | 75 | 1 | 5 | 189 | 683 | 866 | 3.28E-69 | 230 |
| MsG0380017327.01.T04 | Q9SMZ2 | 56.216 | 185 | 81 | 0 | 4 | 188 | 682 | 866 | 9.48E-66 | 221 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017327.01.T01 | A0A392MWV4 | 68.482 | 257 | 81 | 0 | 4 | 260 | 2 | 258 | 2.72e-127 | 375 |
| MsG0380017327.01.T02 | A0A392MWV4 | 73.659 | 205 | 54 | 0 | 1 | 205 | 54 | 258 | 3.68e-107 | 322 |
| MsG0380017327.01.T03 | A0A392MWV4 | 77.041 | 196 | 44 | 1 | 1 | 196 | 64 | 258 | 6.52e-106 | 318 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048856.01 | MsG0380017327.01 | 0.811310 | 7.462673e-51 | 4.603656e-48 |
| MsG0080049148.01 | MsG0380017327.01 | 0.815321 | 9.814702e-52 | 6.745050e-49 |
| MsG0180000145.01 | MsG0380017327.01 | 0.812093 | 5.041323e-51 | 3.175637e-48 |
| MsG0180000178.01 | MsG0380017327.01 | 0.811455 | 6.942082e-51 | 4.299166e-48 |
| MsG0180000470.01 | MsG0380017327.01 | 0.806885 | 6.620354e-50 | 3.634825e-47 |
| MsG0180000828.01 | MsG0380017327.01 | 0.824825 | 6.542413e-54 | 5.860350e-51 |
| MsG0180001141.01 | MsG0380017327.01 | 0.817376 | 3.405569e-52 | 2.475972e-49 |
| MsG0180003073.01 | MsG0380017327.01 | 0.824120 | 9.588854e-54 | 8.416547e-51 |
| MsG0180003097.01 | MsG0380017327.01 | 0.834735 | 2.524395e-56 | 3.022067e-53 |
| MsG0180003188.01 | MsG0380017327.01 | 0.831384 | 1.721092e-55 | 1.864763e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017327.01.T01 | MTR_3g105370 | 68.726 | 259 | 81 | 0 | 2 | 260 | 598 | 856 | 2.60e-123 | 374 |
| MsG0380017327.01.T01 | MTR_3g105370 | 64.583 | 192 | 64 | 3 | 1 | 191 | 496 | 684 | 1.84e-73 | 242 |
| MsG0380017327.01.T01 | MTR_3g105370 | 31.250 | 176 | 87 | 5 | 4 | 162 | 296 | 454 | 1.39e-19 | 88.6 |
| MsG0380017327.01.T01 | MTR_7g417770 | 40.856 | 257 | 152 | 0 | 4 | 260 | 573 | 829 | 2.27e-71 | 235 |
| MsG0380017327.01.T01 | MTR_7g417770 | 27.638 | 199 | 142 | 2 | 2 | 200 | 470 | 666 | 2.71e-17 | 82.0 |
| MsG0380017327.01.T01 | MTR_7g417770 | 27.206 | 272 | 163 | 9 | 5 | 258 | 271 | 525 | 6.18e-17 | 80.9 |
| MsG0380017327.01.T01 | MTR_7g417770 | 23.667 | 300 | 182 | 6 | 4 | 265 | 371 | 661 | 3.43e-15 | 75.5 |
| MsG0380017327.01.T01 | MTR_7g417770 | 26.316 | 171 | 115 | 4 | 4 | 169 | 169 | 333 | 3.73e-12 | 66.6 |
| MsG0380017327.01.T01 | MTR_7g017700 | 40.856 | 257 | 152 | 0 | 4 | 260 | 573 | 829 | 2.27e-71 | 235 |
| MsG0380017327.01.T01 | MTR_7g017700 | 27.638 | 199 | 142 | 2 | 2 | 200 | 470 | 666 | 2.71e-17 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017327.01.T01 | AT4G33170 | 54.400 | 250 | 114 | 0 | 4 | 253 | 617 | 866 | 2.97e-91 | 290 |
| MsG0380017327.01.T01 | AT4G33170 | 26.705 | 176 | 122 | 3 | 21 | 191 | 55 | 228 | 6.59e-15 | 74.7 |
| MsG0380017327.01.T01 | AT4G33170 | 27.273 | 187 | 108 | 2 | 2 | 174 | 312 | 484 | 3.28e-14 | 72.8 |
| MsG0380017327.01.T01 | AT4G20770 | 41.393 | 244 | 143 | 0 | 4 | 247 | 518 | 761 | 3.03e-70 | 230 |
| MsG0380017327.01.T01 | AT4G20770 | 26.966 | 178 | 122 | 2 | 22 | 196 | 266 | 438 | 1.40e-15 | 76.6 |
| MsG0380017327.01.T01 | AT3G02010 | 41.107 | 253 | 148 | 1 | 2 | 253 | 449 | 701 | 3.67e-69 | 228 |
| MsG0380017327.01.T01 | AT3G02010 | 30.108 | 186 | 124 | 4 | 8 | 191 | 354 | 535 | 9.40e-15 | 74.3 |
| MsG0380017327.01.T01 | AT5G09950 | 41.339 | 254 | 146 | 3 | 1 | 251 | 609 | 862 | 4.11e-68 | 227 |
| MsG0380017327.01.T01 | AT5G09950 | 28.289 | 152 | 107 | 2 | 19 | 169 | 321 | 471 | 2.28e-14 | 73.2 |
| MsG0380017327.01.T01 | AT5G09950 | 27.442 | 215 | 151 | 4 | 1 | 212 | 507 | 719 | 2.56e-13 | 70.1 |
Find 91 sgRNAs with CRISPR-Local
Find 177 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATGCTTAATGCTTGTA+GGG | 0.490447 | 3:+94997825 | MsG0380017327.01.T01:CDS |
| AAGAAGTGGATTTAATATCG+TGG | 0.644576 | 3:+94996586 | MsG0380017327.01.T03:intron |
| AAGCAACATCATCCGGCGAA+GGG | 0.615132 | 3:-94997191 | None:intergenic |
| AAGCTGCGGGGTGCTTGGTG+AGG | 0.521591 | 3:+94997031 | MsG0380017327.01.T01:CDS |
| AAGGTGCACTTATTTGTTGC+TGG | 0.364805 | 3:+94998047 | MsG0380017327.01.T01:CDS |
| AATCTCAGGTTCAACTCCAT+AGG | 0.574466 | 3:-94997695 | None:intergenic |
| ACATAAGCCACTGAATCAGA+AGG | 0.602434 | 3:-94997904 | None:intergenic |
| AGAAGGAACCAGGGTTTAGT+TGG | 0.424231 | 3:+94998010 | MsG0380017327.01.T01:CDS |
| AGGTGCACTTATTTGTTGCT+GGG | 0.460526 | 3:+94998048 | MsG0380017327.01.T01:CDS |
| ATCATCAGATGAGACTCGCT+GGG | 0.546122 | 3:+94997274 | MsG0380017327.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAATGCTTAATGCTTGTA+GGG | + | Chr3:94997825-94997844 | MsG0380017327.01.T01:CDS | 30.0% | |
| ATGAAGGCTTATGTTGAAAT+GGG | + | Chr3:94996094-94996113 | MsG0380017327.01.T03:CDS | 30.0% | |
| ATGATTCCGATGTTATTGTT+TGG | + | Chr3:94996284-94996303 | MsG0380017327.01.T03:intron | 30.0% | |
| ATTTAGCCTAATGCATGAAA+GGG | + | Chr3:94996970-94996989 | MsG0380017327.01.T03:intron | 30.0% | |
| CTTGTTCCAAACAATAACAT+CGG | - | Chr3:94996293-94996312 | None:intergenic | 30.0% | |
| GTTCTGTTAATTATGCAAGA+AGG | + | Chr3:94996547-94996566 | MsG0380017327.01.T03:intron | 30.0% | |
| TAAATCAATGAAGAGCCTTA+AGG | - | Chr3:94996658-94996677 | None:intergenic | 30.0% | |
| TAAATGTAAAGAAGGAACCA+GGG | + | Chr3:94998001-94998020 | MsG0380017327.01.T01:CDS | 30.0% | |
| TATTTAGAAGAATGAGCACT+AGG | + | Chr3:94997480-94997499 | MsG0380017327.01.T01:intron | 30.0% | |
| TATTTAGCCTAATGCATGAA+AGG | + | Chr3:94996969-94996988 | MsG0380017327.01.T03:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 94996058 | 94998098 | 94996058 | ID=MsG0380017327.01;Name=MsG0380017327.01 |
| Chr3 | mRNA | 94996994 | 94998098 | 94996994 | ID=MsG0380017327.01.T01;Parent=MsG0380017327.01;Name=MsG0380017327.01.T01;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|0|0|2|0|268 |
| Chr3 | exon | 94996994 | 94997310 | 94996994 | ID=MsG0380017327.01.T01:exon:2142;Parent=MsG0380017327.01.T01 |
| Chr3 | CDS | 94996994 | 94997310 | 94996994 | ID=MsG0380017327.01.T01:cds;Parent=MsG0380017327.01.T01 |
| Chr3 | CDS | 94997609 | 94998098 | 94997609 | ID=MsG0380017327.01.T01:cds;Parent=MsG0380017327.01.T01 |
| Chr3 | mRNA | 94997159 | 94998098 | 94997159 | ID=MsG0380017327.01.T02;Parent=MsG0380017327.01;Name=MsG0380017327.01.T02;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0|0|2|0|213 |
| Chr3 | exon | 94997159 | 94997310 | 94997159 | ID=MsG0380017327.01.T02:exon:2144;Parent=MsG0380017327.01.T02 |
| Chr3 | CDS | 94997159 | 94997310 | 94997159 | ID=MsG0380017327.01.T02:cds;Parent=MsG0380017327.01.T02 |
| Chr3 | CDS | 94997609 | 94998098 | 94997609 | ID=MsG0380017327.01.T02:cds;Parent=MsG0380017327.01.T02 |
| Chr3 | mRNA | 94996058 | 94998098 | 94996058 | ID=MsG0380017327.01.T03;Parent=MsG0380017327.01;Name=MsG0380017327.01.T03;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0|0|2|0|204 |
| Chr3 | exon | 94996058 | 94996184 | 94996058 | ID=MsG0380017327.01.T03:exon:2145;Parent=MsG0380017327.01.T03 |
| Chr3 | exon | 94997611 | 94998098 | 94997611 | ID=MsG0380017327.01.T03:exon:2146;Parent=MsG0380017327.01.T03 |
| Chr3 | CDS | 94996058 | 94996184 | 94996058 | ID=MsG0380017327.01.T03:cds;Parent=MsG0380017327.01.T03 |
| Chr3 | CDS | 94997611 | 94998098 | 94997611 | ID=MsG0380017327.01.T03:cds;Parent=MsG0380017327.01.T03 |
| Chr3 | mRNA | 94996582 | 94998098 | 94996582 | ID=MsG0380017327.01.T04;Parent=MsG0380017327.01;Name=MsG0380017327.01.T04;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0|0|2|0|203 |
| Chr3 | exon | 94996582 | 94996717 | 94996582 | ID=MsG0380017327.01.T04:exon:2147;Parent=MsG0380017327.01.T04 |
| Chr3 | exon | 94997623 | 94998098 | 94997623 | ID=MsG0380017327.01.T04:exon:2148;Parent=MsG0380017327.01.T04 |
| Chr3 | CDS | 94996582 | 94996717 | 94996582 | ID=MsG0380017327.01.T04:cds;Parent=MsG0380017327.01.T04 |
| Chr3 | CDS | 94997623 | 94998098 | 94997623 | ID=MsG0380017327.01.T04:cds;Parent=MsG0380017327.01.T04 |
| Gene Sequence |
>MsG0380017327.01.T04 ATGAAAGAAGTGGATTTAATATCGTGGAACACTGTGTATACGGGTTGTGCTCTGAGTGGTTTAGAGGAGTGTTCCTTAAGGCTCTTCATTGATTTATTACGGAGTGGCCTGTTGCCAGATCAATTCACTATTGCAAGGGTCCTTTCTGCTTGTAGTCATTCTGGTTTGATATCTGATGCATATGAAAATTTTGATTCTATGCAGAAAACCTATGGAGTTGAACCTGAGATTGAGCACTATTCTTGTCTTGTGGATGCCCTTAGCCGTGCGGGGCGCATACAAGAGGCTGAGAAGGTGGTATCATCAATGCCTTTTGAAGCTTCTGCTACAATGTACAGAACAATGCTTAATGCTTGTAGGGTTCAGGGAGACAAAGAGACTGGAAAACGCGTGGCAGAAAAGCTTTTAACCATGGATCCTTCTGATTCAGTGGCTTATGTTCTTTTATCCAATATTTATGCGGCTGCTAACCAATGGGAAAATGCAGTGAGTGCTAGGAATATGATGAAAAGAGTAAATGTAAAGAAGGAACCAGGGTTTAGTTGGATAGACATGAAAACAAGGTGCACTTATTTGTTGCTGGGGATAGATCACATAAAGAAACTGATTTGA >MsG0380017327.01.T01 GAGAAAGCTGATCAGATTACTTTTGCAAATGCGGCCAAAGCTGCGGGGTGCTTGGTGAGGCTTCAGCAAGGGAAGCAGATTCATGCTGTTGTTATAAAAATGAGGTTTCATTATGATTTGTTTGTCATTAGTGGTATTCTGGACATGTATCTGAAATGTGGAGAGATGAAAAGTGCTCGTAAAGTTTTCAATCAGATCCCTTCGCCGGATGATGTTGCTTGGACCACTGTGATTTCGGGATGTGTGGAAAACGGGGAGGAAGAGCAAGCTCTTTTTACATATCATCAGATGAGACTCGCTGGGGTGCAACCGGATGAAGTTACTTTTATTGGGGTCCTTTCTGCTTGTAGTCATTCTGGTTTGATATCTGATGCATATGAAAATTTTGATTCTATGCAGAAAACCTATGGAGTTGAACCTGAGATTGAGCACTATTCTTGTCTTGTGGATGCCCTTAGCCGTGCGGGGCGCATACAAGAGGCTGAGAAGGTGGTATCATCAATGCCTTTTGAAGCTTCTGCTACAATGTACAGAACAATGCTTAATGCTTGTAGGGTTCAGGGAGACAAAGAGACTGGAAAACGCGTGGCAGAAAAGCTTTTAACCATGGATCCTTCTGATTCAGTGGCTTATGTTCTTTTATCCAATATTTATGCGGCTGCTAACCAATGGGAAAATGCAGTGAGTGCTAGGAATATGATGAAAAGAGTAAATGTAAAGAAGGAACCAGGGTTTAGTTGGATAGACATGAAAACAAGGTGCACTTATTTGTTGCTGGGGATAGATCACATAAAGAAACTGATTTGA >MsG0380017327.01.T02 ATGAAAAGTGCTCGTAAAGTTTTCAATCAGATCCCTTCGCCGGATGATGTTGCTTGGACCACTGTGATTTCGGGATGTGTGGAAAACGGGGAGGAAGAGCAAGCTCTTTTTACATATCATCAGATGAGACTCGCTGGGGTGCAACCGGATGAAGTTACTTTTATTGGGGTCCTTTCTGCTTGTAGTCATTCTGGTTTGATATCTGATGCATATGAAAATTTTGATTCTATGCAGAAAACCTATGGAGTTGAACCTGAGATTGAGCACTATTCTTGTCTTGTGGATGCCCTTAGCCGTGCGGGGCGCATACAAGAGGCTGAGAAGGTGGTATCATCAATGCCTTTTGAAGCTTCTGCTACAATGTACAGAACAATGCTTAATGCTTGTAGGGTTCAGGGAGACAAAGAGACTGGAAAACGCGTGGCAGAAAAGCTTTTAACCATGGATCCTTCTGATTCAGTGGCTTATGTTCTTTTATCCAATATTTATGCGGCTGCTAACCAATGGGAAAATGCAGTGAGTGCTAGGAATATGATGAAAAGAGTAAATGTAAAGAAGGAACCAGGGTTTAGTTGGATAGACATGAAAACAAGGTGCACTTATTTGTTGCTGGGGATAGATCACATAAAGAAACTGATTTGA >MsG0380017327.01.T03 ATGCCTGTGAGGGATGTGGTGTTATGGAATGTGATGATGAAGGCTTATGTTGAAATGGGTGCTGGTGATGAAGCGTTGGGTCTTTTTTCTGCATTTCATCGAAGTGGACTGCGTCCTGATTGTGTTATTACTTTTATTGGGGTCCTTTCTGCTTGTAGTCATTCTGGTTTGATATCTGATGCATATGAAAATTTTGATTCTATGCAGAAAACCTATGGAGTTGAACCTGAGATTGAGCACTATTCTTGTCTTGTGGATGCCCTTAGCCGTGCGGGGCGCATACAAGAGGCTGAGAAGGTGGTATCATCAATGCCTTTTGAAGCTTCTGCTACAATGTACAGAACAATGCTTAATGCTTGTAGGGTTCAGGGAGACAAAGAGACTGGAAAACGCGTGGCAGAAAAGCTTTTAACCATGGATCCTTCTGATTCAGTGGCTTATGTTCTTTTATCCAATATTTATGCGGCTGCTAACCAATGGGAAAATGCAGTGAGTGCTAGGAATATGATGAAAAGAGTAAATGTAAAGAAGGAACCAGGGTTTAGTTGGATAGACATGAAAACAAGGTGCACTTATTTGTTGCTGGGGATAGATCACATAAAGAAACTGATTTGA |
| Protein sequence |
>MsG0380017327.01.T04 MKEVDLISWNTVYTGCALSGLEECSLRLFIDLLRSGLLPDQFTIARVLSACSHSGLISDAYENFDSMQKTYGVEPEIEHYSCLVDALSRAGRIQEAEKVVSSMPFEASATMYRTMLNACRVQGDKETGKRVAEKLLTMDPSDSVAYVLLSNIYAAANQWENAVSARNMMKRVNVKKEPGFSWIDMKTRCTYLLLGIDHIKKLI* >MsG0380017327.01.T01 EKADQITFANAAKAAGCLVRLQQGKQIHAVVIKMRFHYDLFVISGILDMYLKCGEMKSARKVFNQIPSPDDVAWTTVISGCVENGEEEQALFTYHQMRLAGVQPDEVTFIGVLSACSHSGLISDAYENFDSMQKTYGVEPEIEHYSCLVDALSRAGRIQEAEKVVSSMPFEASATMYRTMLNACRVQGDKETGKRVAEKLLTMDPSDSVAYVLLSNIYAAANQWENAVSARNMMKRVNVKKEPGFSWIDMKTRCTYLLLGIDHIKKLI* >MsG0380017327.01.T02 MKSARKVFNQIPSPDDVAWTTVISGCVENGEEEQALFTYHQMRLAGVQPDEVTFIGVLSACSHSGLISDAYENFDSMQKTYGVEPEIEHYSCLVDALSRAGRIQEAEKVVSSMPFEASATMYRTMLNACRVQGDKETGKRVAEKLLTMDPSDSVAYVLLSNIYAAANQWENAVSARNMMKRVNVKKEPGFSWIDMKTRCTYLLLGIDHIKKLI* >MsG0380017327.01.T03 MPVRDVVLWNVMMKAYVEMGAGDEALGLFSAFHRSGLRPDCVITFIGVLSACSHSGLISDAYENFDSMQKTYGVEPEIEHYSCLVDALSRAGRIQEAEKVVSSMPFEASATMYRTMLNACRVQGDKETGKRVAEKLLTMDPSDSVAYVLLSNIYAAANQWENAVSARNMMKRVNVKKEPGFSWIDMKTRCTYLLLGIDHIKKLI* |
