AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028000.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028000.01.T01 MTR_5g069640 70.588 51 15 0 31 81 68 118 3.00e-20 81.3
MsG0580028000.01.T01 MTR_8g093450 89.744 39 4 0 96 134 57 95 1.57e-18 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028000.01.T01 AT2G29590 58.824 51 21 0 31 81 65 115 3.10e-13 63.2

Find 30 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCCTCCTCTTTGTTAAAC+TGG 0.287597 5:-72821528 None:intergenic
ATGGATGATGAGAATAATTA+TGG 0.318824 5:+72817137 MsG0580028000.01.T01:CDS
GGGGCTGTAATCCATGAAAA+TGG 0.376151 5:+72821170 MsG0580028000.01.T01:CDS
AAGAAAAGATGAAAAGTGTA+TGG 0.395163 5:+72821563 MsG0580028000.01.T01:CDS
GTATTGTACCTGATACAATA+TGG 0.398826 5:-72821395 None:intergenic
GTGAAAGATAAGAAAGATCT+TGG 0.408538 5:+72821478 MsG0580028000.01.T01:CDS
AGTCATGATTGATATTGATA+TGG 0.416501 5:+72817181 MsG0580028000.01.T01:CDS
GAGGAGAACCATATTGTATC+AGG 0.416982 5:+72821387 MsG0580028000.01.T01:CDS
CATATCAACATAAACATTCA+TGG 0.435825 5:-72821198 None:intergenic
ACTCTCATGTACTTCCTCCA+TGG 0.465684 5:-72817161 None:intergenic
GTTTAACAAAGAGGAGGGAT+TGG 0.488431 5:+72821531 MsG0580028000.01.T01:CDS
AACACTGGAAAGTTGGCTAA+TGG 0.488900 5:+72821116 MsG0580028000.01.T01:CDS
CAATATGGTTCTCCTCTTCA+CGG 0.490112 5:-72821380 None:intergenic
ATAGGACAACACTGGAAAGT+TGG 0.504974 5:+72821109 MsG0580028000.01.T01:intron
CTCTCATGTACTTCCTCCAT+GGG 0.530308 5:-72817160 None:intergenic
GAATATCTTGAGGAATATGT+TGG 0.532265 5:+72821355 MsG0580028000.01.T01:CDS
AGAAAAGATGAAAAGTGTAT+GGG 0.540681 5:+72821564 MsG0580028000.01.T01:CDS
ATATCAACATAAACATTCAT+GGG 0.545871 5:-72821197 None:intergenic
TTTGTTAAACTGGTAATACA+AGG 0.562933 5:-72821518 None:intergenic
TACCAGTTTAACAAAGAGGA+GGG 0.568079 5:+72821526 MsG0580028000.01.T01:CDS
ATGAGAATAATTATGGCCCA+TGG 0.589425 5:+72817144 MsG0580028000.01.T01:CDS
GTATTACCAGTTTAACAAAG+AGG 0.598288 5:+72821522 MsG0580028000.01.T01:CDS
TCTTGGAATCATGAAAGTCA+AGG 0.604539 5:+72821495 MsG0580028000.01.T01:CDS
TTACCAGTTTAACAAAGAGG+AGG 0.617647 5:+72821525 MsG0580028000.01.T01:CDS
CCTCTATTATAGGACAACAC+TGG 0.628025 5:+72821101 MsG0580028000.01.T01:intron
TCACGTGCAGGAATATCTTG+AGG 0.635000 5:+72821345 MsG0580028000.01.T01:intron
AAGATGAAAAGTGTATGGGA+AGG 0.640402 5:+72821568 MsG0580028000.01.T01:CDS
AATATGTTGGAACCGTGAAG+AGG 0.650450 5:+72821368 MsG0580028000.01.T01:CDS
TATCAACATAAACATTCATG+GGG 0.688765 5:-72821196 None:intergenic
AGAATAATTATGGCCCATGG+AGG 0.704372 5:+72817147 MsG0580028000.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTACTTGAAATAAAA+AGG + Chr5:72820733-72820752 MsG0580028000.01.T01:intron 10.0%
!! TATTTATACAAATATCAAAT+AGG - Chr5:72820908-72820927 None:intergenic 10.0%
!!! AATTATGGTTTTATATTATT+TGG + Chr5:72819836-72819855 MsG0580028000.01.T01:intron 10.0%
!! AAGATAAAAGATGAAAATTA+TGG - Chr5:72819050-72819069 None:intergenic 15.0%
!! AATAATCCTTTATTAGTTAA+GGG + Chr5:72820973-72820992 MsG0580028000.01.T01:intron 15.0%
!! AGATTAAATTTAAGAAAAAC+TGG + Chr5:72821030-72821049 MsG0580028000.01.T01:intron 15.0%
!! ATAATATCTATACTCAATTA+TGG + Chr5:72818801-72818820 MsG0580028000.01.T01:intron 15.0%
!! TAATAATCCTTTATTAGTTA+AGG + Chr5:72820972-72820991 MsG0580028000.01.T01:intron 15.0%
!! TATATGAATATGTTGAATTA+TGG + Chr5:72819821-72819840 MsG0580028000.01.T01:intron 15.0%
!! TTATTCTATATAGAAATGAA+TGG + Chr5:72821059-72821078 MsG0580028000.01.T01:intron 15.0%
!! TTTAAACTTGAAATTAACAA+TGG - Chr5:72819230-72819249 None:intergenic 15.0%
!!! ATAAAGGATTATTATTTCTA+TGG - Chr5:72820966-72820985 None:intergenic 15.0%
!!! GATATTATTTTGTCATTATT+AGG - Chr5:72818789-72818808 None:intergenic 15.0%
!!! TTGTAGTGTTTTAATTTTAA+GGG + Chr5:72819490-72819509 MsG0580028000.01.T01:intron 15.0%
!! AACTCTAAACACATAATTTA+TGG - Chr5:72817736-72817755 None:intergenic 20.0%
!! AATAGATTCATAATCACAAT+TGG + Chr5:72819649-72819668 MsG0580028000.01.T01:intron 20.0%
!! ACACACATTATTATCTAATA+AGG - Chr5:72818328-72818347 None:intergenic 20.0%
!! ATATAGAAATGAATGGTAAA+AGG + Chr5:72821066-72821085 MsG0580028000.01.T01:intron 20.0%
!! ATATCAACATAAACATTCAT+GGG - Chr5:72821200-72821219 None:intergenic 20.0%
!! ATGGAAAATCTGAATATTAA+TGG - Chr5:72818598-72818617 None:intergenic 20.0%
!! ATTATTATCTAATAAGGTGA+CGG - Chr5:72818322-72818341 None:intergenic 20.0%
!! CAATGCATAAAAAAGAAAAT+GGG - Chr5:72819157-72819176 None:intergenic 20.0%
!! TAAGATGATGAGAATAATTA+CGG - Chr5:72818169-72818188 None:intergenic 20.0%
!! TCAATGCATAAAAAAGAAAA+TGG - Chr5:72819158-72819177 None:intergenic 20.0%
!! TCATGTTAACATAAAAGATT+AGG + Chr5:72821263-72821282 MsG0580028000.01.T01:intron 20.0%
!! TGTGATTATGAATCTATTAT+AGG - Chr5:72819647-72819666 None:intergenic 20.0%
!!! AAAAAAAGAATTTTGAGAAC+GGG - Chr5:72819890-72819909 None:intergenic 20.0%
!!! AAAAATACAGTTTTGACATT+AGG + Chr5:72817599-72817618 MsG0580028000.01.T01:intron 20.0%
!!! CTTGTAGTGTTTTAATTTTA+AGG + Chr5:72819489-72819508 MsG0580028000.01.T01:intron 20.0%
!!! GAAAAAAAGAATTTTGAGAA+CGG - Chr5:72819891-72819910 None:intergenic 20.0%
!!! TAATTCAACCATAAATTTTG+TGG - Chr5:72817554-72817573 None:intergenic 20.0%
!!! TGGTTTTATATTATTTGGAT+AGG + Chr5:72819841-72819860 MsG0580028000.01.T01:intron 20.0%
!!! TTGAGTTTTAAAATTTTCTG+AGG + Chr5:72819754-72819773 MsG0580028000.01.T01:intron 20.0%
!!! TTTTAAAACTCAACATATGA+TGG - Chr5:72819747-72819766 None:intergenic 20.0%
! AAACAGGAATAGAAAAATAC+AGG - Chr5:72819403-72819422 None:intergenic 25.0%
! AACAGGAATAGAAAAATACA+GGG - Chr5:72819402-72819421 None:intergenic 25.0%
! AAGTAAAGACTAAAGAAAAC+AGG - Chr5:72819975-72819994 None:intergenic 25.0%
! AAGTCTAACCACAAAATTTA+TGG + Chr5:72817543-72817562 MsG0580028000.01.T01:intron 25.0%
! AATCGAGTGAAAAAAAAGTT+TGG + Chr5:72817348-72817367 MsG0580028000.01.T01:intron 25.0%
! AATCTGAATATTAATGGTGA+TGG - Chr5:72818592-72818611 None:intergenic 25.0%
! ACTCAATTATGGAAAAAGAT+TGG + Chr5:72818812-72818831 MsG0580028000.01.T01:intron 25.0%
! AGAAAGATCATGTAATCTAA+AGG - Chr5:72818090-72818109 None:intergenic 25.0%
! AGCAAAATCTTGTGATTTAA+AGG + Chr5:72817216-72817235 MsG0580028000.01.T01:intron 25.0%
! AGTCAAGATCAATATTGATA+TGG - Chr5:72818125-72818144 None:intergenic 25.0%
! AGTCATGATTGATATTGATA+TGG + Chr5:72817181-72817200 MsG0580028000.01.T01:CDS 25.0%
! AGTTATATAGTCATGTCATT+AGG + Chr5:72819590-72819609 MsG0580028000.01.T01:intron 25.0%
! ATCTGAATATTAATGGTGAT+GGG - Chr5:72818591-72818610 None:intergenic 25.0%
! ATCTTCAAGTACAAAAAGTT+CGG - Chr5:72818043-72818062 None:intergenic 25.0%
! ATGGATGATGAGAATAATTA+TGG + Chr5:72817137-72817156 MsG0580028000.01.T01:CDS 25.0%
! ATGTAAAGCTCAAACTATAA+TGG - Chr5:72819694-72819713 None:intergenic 25.0%
! ATTAAATCCATTTGTTTCCA+TGG + Chr5:72818437-72818456 MsG0580028000.01.T01:intron 25.0%
! ATTCATAATCACAATTGGTT+TGG + Chr5:72819654-72819673 MsG0580028000.01.T01:intron 25.0%
! CATATCAACATAAACATTCA+TGG - Chr5:72821201-72821220 None:intergenic 25.0%
! CATTTAATGACAATAGAAGA+TGG - Chr5:72817814-72817833 None:intergenic 25.0%
! CTACAAAAGGTAAATATACA+TGG - Chr5:72818715-72818734 None:intergenic 25.0%
! GCAAAATCTTGTGATTTAAA+GGG + Chr5:72817217-72817236 MsG0580028000.01.T01:intron 25.0%
! TACGATATGGATAAATGAAT+TGG + Chr5:72818484-72818503 MsG0580028000.01.T01:intron 25.0%
! TATCAACATAAACATTCATG+GGG - Chr5:72821199-72821218 None:intergenic 25.0%
! TATGAATCTATTATAGGCAA+AGG - Chr5:72819641-72819660 None:intergenic 25.0%
! TGAAAAAAAAGTTTGGAAGA+AGG + Chr5:72817355-72817374 MsG0580028000.01.T01:intron 25.0%
! TTAAATCCATTTGTTTCCAT+GGG + Chr5:72818438-72818457 MsG0580028000.01.T01:intron 25.0%
! TTTGTTAAACTGGTAATACA+AGG - Chr5:72821521-72821540 None:intergenic 25.0%
!! AAATTTTATATATCACGTGC+AGG + Chr5:72821333-72821352 MsG0580028000.01.T01:intron 25.0%
!! AAGAAAAGATGAAAAGTGTA+TGG + Chr5:72821563-72821582 MsG0580028000.01.T01:CDS 25.0%
!! ACAAATGGATTTAATCTTGA+TGG - Chr5:72818432-72818451 None:intergenic 25.0%
!! AGAAAAGATGAAAAGTGTAT+GGG + Chr5:72821564-72821583 MsG0580028000.01.T01:CDS 25.0%
!! ATTGTAATTTTCTTGTATGC+AGG + Chr5:72817689-72817708 MsG0580028000.01.T01:intron 25.0%
!! ATTTTATGAGTGAGATTTCT+AGG + Chr5:72820473-72820492 MsG0580028000.01.T01:intron 25.0%
!! TAATATTCAGATTTTCCATG+AGG + Chr5:72818599-72818618 MsG0580028000.01.T01:intron 25.0%
!!! AAAAAAGAATTTTGAGAACG+GGG - Chr5:72819889-72819908 None:intergenic 25.0%
!!! AAATTTTCTGAGGATGATTT+AGG + Chr5:72819764-72819783 MsG0580028000.01.T01:intron 25.0%
!!! AATTTTCTGAGGATGATTTA+GGG + Chr5:72819765-72819784 MsG0580028000.01.T01:intron 25.0%
!!! ACACAACTATTGATTTTTCT+AGG + Chr5:72820841-72820860 MsG0580028000.01.T01:intron 25.0%
!!! TCGTAGTATTTTTATTGAGA+AGG - Chr5:72820671-72820690 None:intergenic 25.0%
!!! TCTACTTTGTTTCTAAACTT+TGG + Chr5:72820127-72820146 MsG0580028000.01.T01:intron 25.0%
AAAAGAATGGTGAAAGAAAG+TGG - Chr5:72817949-72817968 None:intergenic 30.0%
AAAAGGTAAATATACATGGC+AGG - Chr5:72818711-72818730 None:intergenic 30.0%
AAAGAATGGTGAAAGAAAGT+GGG - Chr5:72817948-72817967 None:intergenic 30.0%
AAATCTTGTGATTTAAAGGG+AGG + Chr5:72817220-72817239 MsG0580028000.01.T01:intron 30.0%
AAATGTAAGCGTATTGAGTT+GGG + Chr5:72819350-72819369 MsG0580028000.01.T01:intron 30.0%
AACCAATTAAATGCAGGTAA+TGG - Chr5:72817766-72817785 None:intergenic 30.0%
AAGACATAAGTCCAGTAATT+CGG + Chr5:72818530-72818549 MsG0580028000.01.T01:intron 30.0%
AATATCTGTGCTGAAGAAAA+AGG - Chr5:72819451-72819470 None:intergenic 30.0%
ACTTAAAGTAACTTTGAACG+TGG - Chr5:72817911-72817930 None:intergenic 30.0%
AGATCATGTAATCTAAAGGT+AGG - Chr5:72818086-72818105 None:intergenic 30.0%
AGCACAGATATTTAGTAGTT+GGG + Chr5:72819459-72819478 MsG0580028000.01.T01:intron 30.0%
ATCCATTACTGTTAAAGAGT+TGG - Chr5:72818756-72818775 None:intergenic 30.0%
ATCTTTCTTATCTTTCACCT+TGG - Chr5:72821476-72821495 None:intergenic 30.0%
ATTACATGATCTTTCTTCAG+AGG + Chr5:72818094-72818113 MsG0580028000.01.T01:intron 30.0%
ATTACTGGACTTATGTCTTA+TGG - Chr5:72818529-72818548 None:intergenic 30.0%
ATTGACAAAAACTACATGCA+AGG - Chr5:72820339-72820358 None:intergenic 30.0%
CATTAGGGAAACATGAAATA+GGG + Chr5:72819299-72819318 MsG0580028000.01.T01:intron 30.0%
CATTTATCCATATCGTAGAA+AGG - Chr5:72818481-72818500 None:intergenic 30.0%
CTGAAAAGAAGCTTCAATTT+AGG + Chr5:72821421-72821440 MsG0580028000.01.T01:intron 30.0%
GAATATCTTGAGGAATATGT+TGG + Chr5:72821355-72821374 MsG0580028000.01.T01:CDS 30.0%
GATGACTACAACAGATATAT+GGG - Chr5:72818274-72818293 None:intergenic 30.0%
GCTTAGAGTAATGTAAAACA+TGG + Chr5:72820010-72820029 MsG0580028000.01.T01:intron 30.0%
GCTTATAGTAATACTAAGCA+TGG + Chr5:72820293-72820312 MsG0580028000.01.T01:intron 30.0%
GTATTACCAGTTTAACAAAG+AGG + Chr5:72821522-72821541 MsG0580028000.01.T01:CDS 30.0%
GTATTGTACCTGATACAATA+TGG - Chr5:72821398-72821417 None:intergenic 30.0%
GTCAAAATTGTGTATCTTTC+AGG + Chr5:72820174-72820193 MsG0580028000.01.T01:intron 30.0%
GTGAAAGATAAGAAAGATCT+TGG + Chr5:72821478-72821497 MsG0580028000.01.T01:CDS 30.0%
TATGAATCGAGTGAAAAGAA+TGG - Chr5:72817962-72817981 None:intergenic 30.0%
TCAGAAGATTATGTTGTGAT+GGG + Chr5:72819269-72819288 MsG0580028000.01.T01:intron 30.0%
TCATTAGGGAAACATGAAAT+AGG + Chr5:72819298-72819317 MsG0580028000.01.T01:intron 30.0%
TCTTTCTTATCTTTCACCTT+GGG - Chr5:72821475-72821494 None:intergenic 30.0%
TGATGACTACAACAGATATA+TGG - Chr5:72818275-72818294 None:intergenic 30.0%
TGTGTTAATGTGCTATCTTT+AGG + Chr5:72821295-72821314 MsG0580028000.01.T01:intron 30.0%
TTCAGAAGATTATGTTGTGA+TGG + Chr5:72819268-72819287 MsG0580028000.01.T01:intron 30.0%
TTTCACTTTCAATGTACTCT+TGG + Chr5:72820590-72820609 MsG0580028000.01.T01:intron 30.0%
! AAAGTTCGATTTTCGAAAGT+CGG - Chr5:72817853-72817872 None:intergenic 30.0%
! AACTGTATTTTTCTTGTACG+TGG - Chr5:72817591-72817610 None:intergenic 30.0%
! AGAAAACAGGTACGTTTTAT+AGG - Chr5:72819962-72819981 None:intergenic 30.0%
! AGTTATGTCTTTTAGCAATC+AGG + Chr5:72819521-72819540 MsG0580028000.01.T01:intron 30.0%
! ATTAGTTTACTGAACGTCAA+AGG - Chr5:72817667-72817686 None:intergenic 30.0%
! ATTGAGAAGGTTTTCCTTTA+CGG - Chr5:72820658-72820677 None:intergenic 30.0%
! CATGTAGTTTTTGTCAATCA+CGG + Chr5:72820341-72820360 MsG0580028000.01.T01:intron 30.0%
! CGTTCATAACACGTTTTATA+GGG - Chr5:72819933-72819952 None:intergenic 30.0%
! CTATAATGGAGTTCATAGAA+TGG - Chr5:72819680-72819699 None:intergenic 30.0%
! GAAAACAGGTACGTTTTATA+GGG - Chr5:72819961-72819980 None:intergenic 30.0%
! GTTACTTTCACCTCTATTAT+AGG + Chr5:72821091-72821110 MsG0580028000.01.T01:intron 30.0%
! GTTCAAAGTTACTTTAAGTG+TGG + Chr5:72817912-72817931 MsG0580028000.01.T01:intron 30.0%
! TATTTGGTAGTATACAACTG+TGG + Chr5:72820414-72820433 MsG0580028000.01.T01:intron 30.0%
! TCGTTCATAACACGTTTTAT+AGG - Chr5:72819934-72819953 None:intergenic 30.0%
! TTCTTTCAAGATCACCATTT+TGG + Chr5:72818378-72818397 MsG0580028000.01.T01:intron 30.0%
!! AGTAAACTCTGCTTTATTTG+TGG + Chr5:72817284-72817303 MsG0580028000.01.T01:intron 30.0%
!! CTCTTTAACAGTAATGGATT+TGG + Chr5:72818757-72818776 MsG0580028000.01.T01:intron 30.0%
!! TGCTTTATTTGTGTCTCAAT+AGG - Chr5:72818013-72818032 None:intergenic 30.0%
!!! TCTTTTTTTCTGTGTAGTGT+TGG + Chr5:72819901-72819920 MsG0580028000.01.T01:intron 30.0%
AAAAATACCCTTGCCACATA+AGG - Chr5:72818873-72818892 None:intergenic 35.0%
AAAAGTTTGGAAGAAGGTGA+TGG + Chr5:72817361-72817380 MsG0580028000.01.T01:intron 35.0%
AAAATACCCTTGCCACATAA+GGG - Chr5:72818872-72818891 None:intergenic 35.0%
AAGAATGGTGAAAGAAAGTG+GGG - Chr5:72817947-72817966 None:intergenic 35.0%
AAGTAAAGACTGAAGTGAAC+AGG - Chr5:72820260-72820279 None:intergenic 35.0%
AATTATATGCCGCTGCTATT+TGG + Chr5:72820398-72820417 MsG0580028000.01.T01:intron 35.0%
ACTCAATACGCTTACATTTG+TGG - Chr5:72819349-72819368 None:intergenic 35.0%
ACTTATGTCTTATGGTAGAG+TGG - Chr5:72818521-72818540 None:intergenic 35.0%
AGAGGTCATACAATCTTACT+TGG + Chr5:72821000-72821019 MsG0580028000.01.T01:intron 35.0%
AGCCAACTCTTTAACAGTAA+TGG + Chr5:72818751-72818770 MsG0580028000.01.T01:intron 35.0%
ATAGTCATGTCATTAGGTCA+TGG + Chr5:72819596-72819615 MsG0580028000.01.T01:intron 35.0%
ATCAAAGTAGCGAACAAAAC+AGG - Chr5:72819419-72819438 None:intergenic 35.0%
ATGAGAATAATTATGGCCCA+TGG + Chr5:72817144-72817163 MsG0580028000.01.T01:CDS 35.0%
ATGGCATAAGTTCTTGGAAA+AGG - Chr5:72819728-72819747 None:intergenic 35.0%
CAAAGCAATCAGTTAAATGC+AGG + Chr5:72817507-72817526 MsG0580028000.01.T01:intron 35.0%
CAAAGTAGACTAAAGAGAAC+AGG - Chr5:72820116-72820135 None:intergenic 35.0%
CAAATGTAAGCGTATTGAGT+TGG + Chr5:72819349-72819368 MsG0580028000.01.T01:intron 35.0%
CAGCACAGATATTTAGTAGT+TGG + Chr5:72819458-72819477 MsG0580028000.01.T01:intron 35.0%
CATATGATGGCATAAGTTCT+TGG - Chr5:72819734-72819753 None:intergenic 35.0%
CATGTAATCTAAAGGTAGGA+AGG - Chr5:72818082-72818101 None:intergenic 35.0%
CTCACACCCTTAACTAATAA+AGG - Chr5:72820982-72821001 None:intergenic 35.0%
GAAGAGTTTCGGTTAATTGT+TGG + Chr5:72818557-72818576 MsG0580028000.01.T01:intron 35.0%
GACCATTACCTGCATTTAAT+TGG + Chr5:72817761-72817780 MsG0580028000.01.T01:intron 35.0%
GATCTTGAAAGAATCCTTGA+GGG - Chr5:72818371-72818390 None:intergenic 35.0%
GATGGAGAACAACAAATTCA+TGG - Chr5:72818414-72818433 None:intergenic 35.0%
GGTCAACTGTGACTTTAATT+CGG + Chr5:72817710-72817729 MsG0580028000.01.T01:intron 35.0%
TAAAGTAACTTTGAACGTGG+TGG - Chr5:72817908-72817927 None:intergenic 35.0%
TACCAGTTTAACAAAGAGGA+GGG + Chr5:72821526-72821545 MsG0580028000.01.T01:CDS 35.0%
TAGTCATGTCATTAGGTCAT+GGG + Chr5:72819597-72819616 MsG0580028000.01.T01:intron 35.0%
TATAACTCTAACCATTGCAC+TGG - Chr5:72819577-72819596 None:intergenic 35.0%
TCAAAATTGTGTTTCCTTGC+AGG + Chr5:72820036-72820055 MsG0580028000.01.T01:intron 35.0%
TCTTGGAATCATGAAAGTCA+AGG + Chr5:72821495-72821514 MsG0580028000.01.T01:CDS 35.0%
TCTTGGATTTGTGTATCTAG+AGG + Chr5:72820607-72820626 MsG0580028000.01.T01:intron 35.0%
TGATCTTGAAAGAATCCTTG+AGG - Chr5:72818372-72818391 None:intergenic 35.0%
TGTATACTACCAAATAGCAG+CGG - Chr5:72820410-72820429 None:intergenic 35.0%
TTAAATGCAGGAAACAACCA+TGG + Chr5:72817519-72817538 MsG0580028000.01.T01:intron 35.0%
TTACCAGTTTAACAAAGAGG+AGG + Chr5:72821525-72821544 MsG0580028000.01.T01:CDS 35.0%
TTGTTGGATTGTTTGCGATT+TGG + Chr5:72820780-72820799 MsG0580028000.01.T01:intron 35.0%
TTTACTGAACGTCAAAGGTT+TGG - Chr5:72817662-72817681 None:intergenic 35.0%
TTTGACATTAGGTAAACCAG+TGG + Chr5:72817610-72817629 MsG0580028000.01.T01:intron 35.0%
! AAGATGAAAAGTGTATGGGA+AGG + Chr5:72821568-72821587 MsG0580028000.01.T01:CDS 35.0%
! AGAAATTGGGTTTTTGTCGT+CGG + Chr5:72820703-72820722 MsG0580028000.01.T01:intron 35.0%
! AGATTATGTTGTGATGGGAT+CGG + Chr5:72819274-72819293 MsG0580028000.01.T01:intron 35.0%
! ATCACAAGATTTTGCTTCTG+AGG - Chr5:72817212-72817231 None:intergenic 35.0%
! ATCAGCTACGACAAGATTTT+CGG + Chr5:72819118-72819137 MsG0580028000.01.T01:intron 35.0%
! ATTATCTAATAAGGTGACGG+TGG - Chr5:72818319-72818338 None:intergenic 35.0%
! ATTTGATAATACTGCCTGCA+AGG - Chr5:72820053-72820072 None:intergenic 35.0%
! GCGATTTTGTTGAATGTTGT+TGG + Chr5:72820764-72820783 MsG0580028000.01.T01:intron 35.0%
! TAACACGTTTTATAGGGAAG+TGG - Chr5:72819927-72819946 None:intergenic 35.0%
! TCAACTTTTGTGAGTGAAGT+TGG + Chr5:72821146-72821165 MsG0580028000.01.T01:CDS 35.0%
! TTAAATGCAGGTAATGGTCA+TGG - Chr5:72817760-72817779 None:intergenic 35.0%
! TTATCTAATAAGGTGACGGT+GGG - Chr5:72818318-72818337 None:intergenic 35.0%
! TTATTAGTTAAGGGTGTGAG+AGG + Chr5:72820982-72821001 MsG0580028000.01.T01:intron 35.0%
! TTTTGTGGTTAGACTTTCCA+TGG - Chr5:72817539-72817558 None:intergenic 35.0%
!! TGAATATTAATGGTGATGGG+TGG - Chr5:72818588-72818607 None:intergenic 35.0%
AAAAACAACCCATCAACTGG+AGG - Chr5:72820573-72820592 None:intergenic 40.0%
AAACGTCACTGCTCTACAAA+AGG - Chr5:72818728-72818747 None:intergenic 40.0%
AAATACCCTTGCCACATAAG+GGG - Chr5:72818871-72818890 None:intergenic 40.0%
AAGCACGAACCAGAGAAATT+GGG + Chr5:72820690-72820709 MsG0580028000.01.T01:intron 40.0%
AATATGTTGGAACCGTGAAG+AGG + Chr5:72821368-72821387 MsG0580028000.01.T01:CDS 40.0%
ACTCTCATGTATTTCCTCCA+AGG + Chr5:72818142-72818161 MsG0580028000.01.T01:intron 40.0%
AGAATAATTATGGCCCATGG+AGG + Chr5:72817147-72817166 MsG0580028000.01.T01:CDS 40.0%
AGGTAAATATACATGGCAGG+TGG - Chr5:72818708-72818727 None:intergenic 40.0%
ATAGGACAACACTGGAAAGT+TGG + Chr5:72821109-72821128 MsG0580028000.01.T01:intron 40.0%
ATCCCTCCTCTTTGTTAAAC+TGG - Chr5:72821531-72821550 None:intergenic 40.0%
ATGAGAATAATTACGGCCCT+TGG - Chr5:72818162-72818181 None:intergenic 40.0%
CAACGCAACCAATTAAATGC+AGG - Chr5:72817772-72817791 None:intergenic 40.0%
CAATATGGTTCTCCTCTTCA+CGG - Chr5:72821383-72821402 None:intergenic 40.0%
CAATTAACCGAAACTCTTCC+AGG - Chr5:72818556-72818575 None:intergenic 40.0%
CCAGTGTTGTCCTATAATAG+AGG - Chr5:72821104-72821123 None:intergenic 40.0%
CCTAACAACAACTTACCTGT+CGG + Chr5:72817878-72817897 MsG0580028000.01.T01:intron 40.0%
CCTCTATTATAGGACAACAC+TGG + Chr5:72821101-72821120 MsG0580028000.01.T01:intron 40.0%
CGACAAAAACCCAATTTCTC+TGG - Chr5:72820702-72820721 None:intergenic 40.0%
CGACTCACAAAATTCATGTC+TGG - Chr5:72819379-72819398 None:intergenic 40.0%
CTAGAGGAATACATATCTCG+TGG + Chr5:72820623-72820642 MsG0580028000.01.T01:intron 40.0%
CTCTCATGTATTTCCTCCAA+GGG + Chr5:72818143-72818162 MsG0580028000.01.T01:intron 40.0%
CTTGTGATTTAAAGGGAGGA+AGG + Chr5:72817224-72817243 MsG0580028000.01.T01:intron 40.0%
GAGATCTACGAAAGAGAAGA+GGG - Chr5:72818924-72818943 None:intergenic 40.0%
GAGATTGAGACCTTCAAACA+CGG - Chr5:72818966-72818985 None:intergenic 40.0%
GAGGAGAACCATATTGTATC+AGG + Chr5:72821387-72821406 MsG0580028000.01.T01:CDS 40.0%
GATTGAGCCTTTCTACGATA+TGG + Chr5:72818471-72818490 MsG0580028000.01.T01:intron 40.0%
GGGTTGTCACATATGCATAA+TGG - Chr5:72818197-72818216 None:intergenic 40.0%
GGTTTCATGAACACAAGTGT+AGG - Chr5:72818229-72818248 None:intergenic 40.0%
GTCATGACTTGATATACCCA+AGG + Chr5:72821456-72821475 MsG0580028000.01.T01:intron 40.0%
GTGAAAAACAACCCATCAAC+TGG - Chr5:72820576-72820595 None:intergenic 40.0%
GTGTAGAGTAATGTGAAGCA+TGG + Chr5:72820149-72820168 MsG0580028000.01.T01:intron 40.0%
GTTTAACAAAGAGGAGGGAT+TGG + Chr5:72821531-72821550 MsG0580028000.01.T01:CDS 40.0%
TAAATATACATGGCAGGTGG+TGG - Chr5:72818705-72818724 None:intergenic 40.0%
TCAATCCAATTTAGCACCCA+TGG - Chr5:72818457-72818476 None:intergenic 40.0%
TCATGTTCATCAGCTCTCAT+TGG - Chr5:72818250-72818269 None:intergenic 40.0%
TGTTGACCTTGAATCGATTG+TGG - Chr5:72819003-72819022 None:intergenic 40.0%
TGTTTCCATGGGTGCTAAAT+TGG + Chr5:72818449-72818468 MsG0580028000.01.T01:intron 40.0%
TTACTGTTAGCGCAGAGATA+AGG - Chr5:72817387-72817406 None:intergenic 40.0%
TTAGCACCCATGGAAACAAA+TGG - Chr5:72818447-72818466 None:intergenic 40.0%
TTCGAAAGTCGGAAAGAAGA+TGG - Chr5:72817842-72817861 None:intergenic 40.0%
! ACAGGTAAGTTGTTGTTAGG+TGG - Chr5:72817878-72817897 None:intergenic 40.0%
! ACTTTTGTGAGTGAAGTTGG+AGG + Chr5:72821149-72821168 MsG0580028000.01.T01:CDS 40.0%
! CTTTTGTGAGTGAAGTTGGA+GGG + Chr5:72821150-72821169 MsG0580028000.01.T01:CDS 40.0%
! GAAATTGGGTTTTTGTCGTC+GGG + Chr5:72820704-72820723 MsG0580028000.01.T01:intron 40.0%
! GATTTTCCATGAGGAAGAAG+AGG + Chr5:72818608-72818627 MsG0580028000.01.T01:intron 40.0%
! TTTTGTGAGTGAAGTTGGAG+GGG + Chr5:72821151-72821170 MsG0580028000.01.T01:CDS 40.0%
!!! TTCGTTGCTTTTTTGCTCTG+TGG + Chr5:72820516-72820535 MsG0580028000.01.T01:intron 40.0%
AACCCATCAACTGGAGGAAA+AGG - Chr5:72820567-72820586 None:intergenic 45.0%
AATTCGGCCTGGAAGAGTTT+CGG + Chr5:72818546-72818565 MsG0580028000.01.T01:intron 45.0%
ACCTTCAAACACGGTTGAGA+GGG - Chr5:72818957-72818976 None:intergenic 45.0%
ACTCTCATGTACTTCCTCCA+TGG - Chr5:72817164-72817183 None:intergenic 45.0%
ACTGAACGTCAAAGGTTTGG+TGG - Chr5:72817659-72817678 None:intergenic 45.0%
AGAATAATTACGGCCCTTGG+AGG - Chr5:72818159-72818178 None:intergenic 45.0%
AGTGTATGGGAAGGACCATA+AGG + Chr5:72821577-72821596 MsG0580028000.01.T01:CDS 45.0%
ATAAGTCCAGTAATTCGGCC+TGG + Chr5:72818535-72818554 MsG0580028000.01.T01:intron 45.0%
ATACACCCCTTATGTGGCAA+GGG + Chr5:72818863-72818882 MsG0580028000.01.T01:intron 45.0%
ATGGAAGAGTGGTATGGAGA+AGG - Chr5:72818845-72818864 None:intergenic 45.0%
ATGGTCCAGCTGTTCCAAAA+TGG - Chr5:72818395-72818414 None:intergenic 45.0%
CTCTCATGTACTTCCTCCAT+GGG - Chr5:72817163-72817182 None:intergenic 45.0%
GAAGCACGAACCAGAGAAAT+TGG + Chr5:72820689-72820708 MsG0580028000.01.T01:intron 45.0%
GGAGATCTACGAAAGAGAAG+AGG - Chr5:72818925-72818944 None:intergenic 45.0%
GGGGCTGTAATCCATGAAAA+TGG + Chr5:72821170-72821189 MsG0580028000.01.T01:CDS 45.0%
GTAGACATGAACTACCCTCA+AGG + Chr5:72818354-72818373 MsG0580028000.01.T01:intron 45.0%
TAAATCGGCGAGTAAGTGGT+TGG + Chr5:72817422-72817441 MsG0580028000.01.T01:intron 45.0%
TACCTTCCTCTTCTTCCTCA+TGG - Chr5:72818617-72818636 None:intergenic 45.0%
TAGGTCATGGGTTTCAGTCA+TGG + Chr5:72819609-72819628 MsG0580028000.01.T01:intron 45.0%
TCACGTGCAGGAATATCTTG+AGG + Chr5:72821345-72821364 MsG0580028000.01.T01:intron 45.0%
TCTTCCATACACCCCTTATG+TGG + Chr5:72818857-72818876 MsG0580028000.01.T01:intron 45.0%
TGGAAGAGTGGTATGGAGAA+GGG - Chr5:72818844-72818863 None:intergenic 45.0%
TTCCATGAGGAAGAAGAGGA+AGG + Chr5:72818612-72818631 MsG0580028000.01.T01:intron 45.0%
! AGCCTTTTCCTCCAGTTGAT+GGG + Chr5:72820562-72820581 MsG0580028000.01.T01:intron 45.0%
! ATAAGGGGTGTATGGAAGAG+TGG - Chr5:72818856-72818875 None:intergenic 45.0%
! CCGACAGGTAAGTTGTTGTT+AGG - Chr5:72817881-72817900 None:intergenic 45.0%
! GTGATGGGATCGGATCATTA+GGG + Chr5:72819284-72819303 MsG0580028000.01.T01:intron 45.0%
! TAGCCTTTTCCTCCAGTTGA+TGG + Chr5:72820561-72820580 MsG0580028000.01.T01:intron 45.0%
! TCAGAGCCACAATCGATTCA+AGG + Chr5:72818994-72819013 MsG0580028000.01.T01:intron 45.0%
! TCATGGGGAGACCATTTTCA+TGG - Chr5:72821184-72821203 None:intergenic 45.0%
! TGTGATGGGATCGGATCATT+AGG + Chr5:72819283-72819302 MsG0580028000.01.T01:intron 45.0%
! TTGAATCGATTGTGGCTCTG+AGG - Chr5:72818995-72819014 None:intergenic 45.0%
!! AGGCTGTTTTCTTCCAGGAA+GGG + Chr5:72819541-72819560 MsG0580028000.01.T01:intron 45.0%
!! CAATCAGGCTGTTTTCTTCC+AGG + Chr5:72819536-72819555 MsG0580028000.01.T01:intron 45.0%
!! TCAGGCAGCATTGACAATTG+CGG + Chr5:72820192-72820211 MsG0580028000.01.T01:intron 45.0%
!! TGAATCGATTGTGGCTCTGA+GGG - Chr5:72818994-72819013 None:intergenic 45.0%
!!! GATCACCATTTTGGAACAGC+TGG + Chr5:72818387-72818406 MsG0580028000.01.T01:intron 45.0%
!!! TTGCTTTTTTGCTCTGTGGC+AGG + Chr5:72820520-72820539 MsG0580028000.01.T01:intron 45.0%
AACAGTAACTCTGCAGGTGG+TGG + Chr5:72817399-72817418 MsG0580028000.01.T01:intron 50.0%
ACGGTTGAGAGGGAGAAGAA+AGG - Chr5:72818947-72818966 None:intergenic 50.0%
ACTCTTCCAGGCCGAATTAC+TGG - Chr5:72818544-72818563 None:intergenic 50.0%
ATCCAAGCACGCAAGGTTCT+AGG + Chr5:72819081-72819100 MsG0580028000.01.T01:intron 50.0%
CATACACCCCTTATGTGGCA+AGG + Chr5:72818862-72818881 MsG0580028000.01.T01:intron 50.0%
CGGTTGAGAGGGAGAAGAAA+GGG - Chr5:72818946-72818965 None:intergenic 50.0%
CGTGGATTTGCGAACCGTAA+AGG + Chr5:72820641-72820660 MsG0580028000.01.T01:intron 50.0%
GAACACAAGTGTAGGCGCAT+TGG - Chr5:72818221-72818240 None:intergenic 50.0%
GACCACCGGAATTTGAACTC+CGG - Chr5:72817455-72817474 None:intergenic 50.0%
GACCTTCAAACACGGTTGAG+AGG - Chr5:72818958-72818977 None:intergenic 50.0%
GCTAACAGTAACTCTGCAGG+TGG + Chr5:72817396-72817415 MsG0580028000.01.T01:intron 50.0%
GGAGTTCAAATTCCGGTGGT+CGG + Chr5:72817454-72817473 MsG0580028000.01.T01:intron 50.0%
GGGTGTATGGAAGAGTGGTA+TGG - Chr5:72818851-72818870 None:intergenic 50.0%
GTCATCTTCTTTCCGACCAC+CGG - Chr5:72817469-72817488 None:intergenic 50.0%
GTGGTAAATCGGCGAGTAAG+TGG + Chr5:72817418-72817437 MsG0580028000.01.T01:intron 50.0%
TCCAAGCACGCAAGGTTCTA+GGG + Chr5:72819082-72819101 MsG0580028000.01.T01:intron 50.0%
TCCACCAACGCAGCAATCTA+GGG + Chr5:72817633-72817652 MsG0580028000.01.T01:intron 50.0%
TCCCTCTCAACCGTGTTTGA+AGG + Chr5:72818953-72818972 MsG0580028000.01.T01:intron 50.0%
TGCGCTAACAGTAACTCTGC+AGG + Chr5:72817393-72817412 MsG0580028000.01.T01:intron 50.0%
! ACAAGTGTAGGCGCATTGGT+GGG - Chr5:72818217-72818236 None:intergenic 50.0%
! CTCTGCAGGTGGTGGTAAAT+CGG + Chr5:72817407-72817426 MsG0580028000.01.T01:intron 50.0%
! CTTGCCACATAAGGGGTGTA+TGG - Chr5:72818864-72818883 None:intergenic 50.0%
!! CAGGCTGTTTTCTTCCAGGA+AGG + Chr5:72819540-72819559 MsG0580028000.01.T01:intron 50.0%
!! GGCTGTTTTCTTCCAGGAAG+GGG + Chr5:72819542-72819561 MsG0580028000.01.T01:intron 50.0%
ACCCTAGAACCTTGCGTGCT+TGG - Chr5:72819086-72819105 None:intergenic 55.0%
CACAAGTGTAGGCGCATTGG+TGG - Chr5:72818218-72818237 None:intergenic 55.0%
GAACGTGGTGGTAAACCGAC+AGG - Chr5:72817896-72817915 None:intergenic 55.0%
GGTGGCCGGAGTTCAAATTC+CGG + Chr5:72817447-72817466 MsG0580028000.01.T01:intron 55.0%
GTCCACCAACGCAGCAATCT+AGG + Chr5:72817632-72817651 MsG0580028000.01.T01:intron 55.0%
TCGGCGAGTAAGTGGTTGGT+AGG + Chr5:72817426-72817445 MsG0580028000.01.T01:intron 55.0%
TCTCGCAATCCAAGCACGCA+AGG + Chr5:72819074-72819093 MsG0580028000.01.T01:intron 55.0%
TTCTTCCAGGAAGGGGACCT+CGG + Chr5:72819549-72819568 MsG0580028000.01.T01:intron 55.0%
! TAACCATTGCACTGGCACCG+AGG - Chr5:72819569-72819588 None:intergenic 55.0%
!! GCGAGTAAGTGGTTGGTAGG+TGG + Chr5:72817429-72817448 MsG0580028000.01.T01:intron 55.0%
!! GTAAGTGGTTGGTAGGTGGC+CGG + Chr5:72817433-72817452 MsG0580028000.01.T01:intron 55.0%
GCCCTAGATTGCTGCGTTGG+TGG - Chr5:72817637-72817656 None:intergenic 60.0%
GGCCGGAGTTCAAATTCCGG+TGG + Chr5:72817450-72817469 MsG0580028000.01.T01:intron 60.0%
GTGGCCCTAGATTGCTGCGT+TGG - Chr5:72817640-72817659 None:intergenic 60.0%
! TTGCTGCGTTGGTGGACCAC+TGG - Chr5:72817629-72817648 None:intergenic 60.0%
!! GGACCTCGGTGCCAGTGCAA+TGG + Chr5:72819563-72819582 MsG0580028000.01.T01:intron 65.0%
! TGGCACCGAGGTCCCCTTCC+TGG - Chr5:72819557-72819576 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 72817137 72821597 72817137 ID=MsG0580028000.01;Name=MsG0580028000.01
Chr5 mRNA 72817137 72821597 72817137 ID=MsG0580028000.01.T01;Parent=MsG0580028000.01;Name=MsG0580028000.01.T01;_AED=0.56;_eAED=0.56;_QI=0|0.33|0.25|0.75|1|1|4|0|134
Chr5 exon 72817137 72817226 72817137 ID=MsG0580028000.01.T01:exon:7340;Parent=MsG0580028000.01.T01
Chr5 exon 72821113 72821253 72821113 ID=MsG0580028000.01.T01:exon:7341;Parent=MsG0580028000.01.T01
Chr5 exon 72821355 72821408 72821355 ID=MsG0580028000.01.T01:exon:7342;Parent=MsG0580028000.01.T01
Chr5 exon 72821478 72821597 72821478 ID=MsG0580028000.01.T01:exon:7343;Parent=MsG0580028000.01.T01
Chr5 CDS 72817137 72817226 72817137 ID=MsG0580028000.01.T01:cds;Parent=MsG0580028000.01.T01
Chr5 CDS 72821113 72821253 72821113 ID=MsG0580028000.01.T01:cds;Parent=MsG0580028000.01.T01
Chr5 CDS 72821355 72821408 72821355 ID=MsG0580028000.01.T01:cds;Parent=MsG0580028000.01.T01
Chr5 CDS 72821478 72821597 72821478 ID=MsG0580028000.01.T01:cds;Parent=MsG0580028000.01.T01
Gene Sequence

>MsG0580028000.01.T01

ATGGATGATGAGAATAATTATGGCCCATGGAGGAAGTACATGAGAGTCATGATTGATATTGATATGGAAGAACCTCAGAAGCAAAATCTTGACAACACTGGAAAGTTGGCTAATGGTGCAATTTCAACTTTTGTGAGTGAAGTTGGAGGGGCTGTAATCCATGAAAATGGTCTCCCCATGAATGTTTATGTTGATATGTCTATTTCTTTTCTTGCAACTGCTCATGTTAGTGAATATCTTGAGGAATATGTTGGAACCGTGAAGAGGAGAACCATATTGTATCAGGTGAAAGATAAGAAAGATCTTGGAATCATGAAAGTCAAGGCCTTGTATTACCAGTTTAACAAAGAGGAGGGATTGGTTGTACTAGAAGAAAAGATGAAAAGTGTATGGGAAGGACCATAA
Protein sequence

>MsG0580028000.01.T01

MDDENNYGPWRKYMRVMIDIDMEEPQKQNLDNTGKLANGAISTFVSEVGGAVIHENGLPMNVYVDMSISFLATAHVSEYLEEYVGTVKRRTILYQVKDKKDLGIMKVKALYYQFNKEEGLVVLEEKMKSVWEGP*