AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045119.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045119.01.T01 MTR_3g077720 82.927 164 20 1 1 156 1 164 5.12e-96 274
MsG0880045119.01.T01 MTR_3g077730 77.099 131 20 2 32 152 5 135 9.97e-64 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045119.01.T01 AT4G22220 65.359 153 43 3 5 148 7 158 1.09e-63 193
MsG0880045119.01.T01 AT4G04080 58.861 158 55 3 1 149 1 157 1.04e-61 188
MsG0880045119.01.T01 AT3G01020 56.707 164 60 2 1 156 3 163 7.07e-57 175

Find 57 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTAAGGTTGATGATAA+TGG 0.228001 8:-54480742 MsG0880045119.01.T01:CDS
TGTCATGAAGTTTCAAATTA+AGG 0.237273 8:-54480755 MsG0880045119.01.T01:CDS
AGTCGCCAATTCCAGCAATT+AGG 0.263293 8:-54480879 MsG0880045119.01.T01:CDS
GATTCTTGCTTTAAGACCTT+TGG 0.331092 8:-54480709 MsG0880045119.01.T01:CDS
TGTTTGAATGGTTTGTTTGT+AGG 0.334004 8:-54478668 MsG0880045119.01.T01:three_prime_UTR
TGCATGATTTCTCCCATTGC+TGG 0.335905 8:-54478920 MsG0880045119.01.T01:exon
GTCGCCAATTCCAGCAATTA+GGG 0.344413 8:-54480878 MsG0880045119.01.T01:CDS
ATGCTGCAGTGAAGCTTAAC+TGG 0.354352 8:+54479033 None:intergenic
TGCAGCTACTGAATGGGTTA+AGG 0.358960 8:-54480509 MsG0880045119.01.T01:intron
TTCTCCCATTGCTGGAATGC+TGG 0.368918 8:-54478912 MsG0880045119.01.T01:three_prime_UTR
GATGAAGTTTCTTGTTTGAA+TGG 0.380469 8:-54478680 MsG0880045119.01.T01:three_prime_UTR
GTCTTTATGACAATACAATA+TGG 0.381045 8:+54478724 None:intergenic
GCGACTCAAACGACGCCGTT+TGG 0.381388 8:+54480896 None:intergenic
AGAACCCTAATTGCTGGAAT+TGG 0.383969 8:+54480874 None:intergenic
GCAGCTACTGAATGGGTTAA+GGG 0.384513 8:-54480508 MsG0880045119.01.T01:intron
GGTGGATCACTACGAAAATA+AGG 0.387972 8:-54480827 MsG0880045119.01.T01:CDS
CGTGGAAGAACCCTAATTGC+TGG 0.397022 8:+54480868 None:intergenic
TACAAATACTTGTGTCGGCT+TGG 0.419718 8:-54478757 MsG0880045119.01.T01:three_prime_UTR
CTTATAAATACTTGTATCGT+TGG 0.431923 8:-54478702 MsG0880045119.01.T01:three_prime_UTR
GATGTGGGATCTGCTCTTGT+TGG 0.435958 8:-54480796 MsG0880045119.01.T01:CDS
TGCCTCGGCTACTGCGGCCA+CGG 0.446467 8:-54478959 MsG0880045119.01.T01:CDS
CGCAAAGAAACTCGTCCAAA+CGG 0.447336 8:-54480911 MsG0880045119.01.T01:CDS
TGCTTTAAGACCTTTGGATG+TGG 0.461939 8:-54480703 MsG0880045119.01.T01:CDS
GACGTTATAACAATACAATA+TGG 0.491794 8:+54478781 None:intergenic
ACTTGTGTCGGCTTGGAGCT+AGG 0.504527 8:-54478750 MsG0880045119.01.T01:three_prime_UTR
CATTCCAGCATTCCAGCAAT+GGG 0.505665 8:+54478908 None:intergenic
GCCTCGGCTACTGCGGCCAC+GGG 0.517239 8:-54478958 MsG0880045119.01.T01:CDS
TCATTCCAGCATTCCAGCAA+TGG 0.517945 8:+54478907 None:intergenic
ATTCTACCACGAGACGGTGG+TGG 0.539129 8:-54480845 MsG0880045119.01.T01:CDS
CTACGAAAATAAGGAAGAGC+GGG 0.540115 8:-54480818 MsG0880045119.01.T01:CDS
TGCAGTGAAGCTTAACTGGC+GGG 0.542392 8:+54479037 None:intergenic
CGTCTTACAAATACTTGTGT+CGG 0.543906 8:-54478762 MsG0880045119.01.T01:three_prime_UTR
TCCCGTGGCCGCAGTAGCCG+AGG 0.550070 8:+54478957 None:intergenic
AATCCACTCATTAGCACCAT+TGG 0.552447 8:+54478856 None:intergenic
AAATAAGGAAGAGCGGGATG+TGG 0.555570 8:-54480812 MsG0880045119.01.T01:CDS
GCTATTGCTTCATCGTCCGT+TGG 0.572466 8:-54480676 MsG0880045119.01.T01:intron
TTATGAAGCTAAGCGTGCCT+CGG 0.575006 8:-54478974 MsG0880045119.01.T01:CDS
AATAAGGAAGAGCGGGATGT+GGG 0.577997 8:-54480811 MsG0880045119.01.T01:CDS
CTTGTTGGTGCACCCGCTTG+TGG 0.585089 8:-54480781 MsG0880045119.01.T01:CDS
CTTCATGACATCACCACAAG+CGG 0.586806 8:+54480768 None:intergenic
TAAGCGTGCCTCGGCTACTG+CGG 0.598393 8:-54478965 MsG0880045119.01.T01:CDS
AGTGATCCACCACCGTCTCG+TGG 0.599249 8:+54480839 None:intergenic
TTCATGACATCACCACAAGC+GGG 0.599308 8:+54480769 None:intergenic
TCATTAGTATGATTCTCCAA+TGG 0.600860 8:-54478872 MsG0880045119.01.T01:three_prime_UTR
TCACTGCAGCATGCTTGCTG+AGG 0.608636 8:-54479022 MsG0880045119.01.T01:CDS
GGTTAAGGGAAAGCAAATTG+AGG 0.612867 8:-54480494 MsG0880045119.01.T01:CDS
AGTGGCAGATTTGTCTCCCG+TGG 0.628353 8:+54478942 None:intergenic
CTGCAGTGAAGCTTAACTGG+CGG 0.631259 8:+54479036 None:intergenic
ACCGTCTCGTGGTAGAATCG+TGG 0.633041 8:+54480850 None:intergenic
TCTCCAATGGTGCTAATGAG+TGG 0.639727 8:-54478859 MsG0880045119.01.T01:three_prime_UTR
AATAGCGGAACCACATCCAA+AGG 0.639771 8:+54480693 None:intergenic
ACTACGAAAATAAGGAAGAG+CGG 0.649525 8:-54480819 MsG0880045119.01.T01:CDS
TCCACGATTCTACCACGAGA+CGG 0.664442 8:-54480851 MsG0880045119.01.T01:CDS
CAATGGGAGAAATCATGCAG+TGG 0.672265 8:+54478924 None:intergenic
AACGGACGATGAAGCAATAG+CGG 0.675366 8:+54480678 None:intergenic
TGAGGATGCTATAAAAGCAG+CGG 0.695297 8:-54479004 MsG0880045119.01.T01:CDS
ACGATTCTACCACGAGACGG+TGG 0.736423 8:-54480848 MsG0880045119.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAAAACTTGATAT+AGG + Chr8:54479323-54479342 None:intergenic 10.0%
!! ATAATCAAATTCAATAAAAA+AGG + Chr8:54478981-54479000 None:intergenic 10.0%
!! TAATCAAATTCAATAAAAAA+GGG + Chr8:54478980-54478999 None:intergenic 10.0%
!!! AAGGATTTTTTTTTTTTTTA+GGG - Chr8:54478935-54478954 MsG0880045119.01.T01:exon 10.0%
!!! TAAGGATTTTTTTTTTTTTT+AGG - Chr8:54478934-54478953 MsG0880045119.01.T01:exon 10.0%
!! TTATATTTATTCTTCATGAT+CGG - Chr8:54480138-54480157 MsG0880045119.01.T01:intron 15.0%
!!! AATAGATACATAATTTTTAG+TGG - Chr8:54480246-54480265 MsG0880045119.01.T01:intron 15.0%
!!! AGTGTAACTTATTTTATTTT+AGG + Chr8:54479920-54479939 None:intergenic 15.0%
!!! ATATAAATTTTTGTTGAAGA+TGG - Chr8:54479412-54479431 MsG0880045119.01.T01:intron 15.0%
!!! TATAAATTTTTGTTGAAGAT+GGG - Chr8:54479413-54479432 MsG0880045119.01.T01:intron 15.0%
!!! TCCCAAATTTTAATAAATTT+GGG + Chr8:54480227-54480246 None:intergenic 15.0%
!!! TTCCCAAATTTTAATAAATT+TGG + Chr8:54480228-54480247 None:intergenic 15.0%
!!! TTGGTTTTAATTTTATGTTT+GGG - Chr8:54479480-54479499 MsG0880045119.01.T01:intron 15.0%
!! ATGTAGAAAGCAAATATATT+TGG - Chr8:54479457-54479476 MsG0880045119.01.T01:intron 20.0%
!! GCCCAAATTTATTAAAATTT+GGG - Chr8:54480223-54480242 MsG0880045119.01.T01:intron 20.0%
!! TGAAGTTAAAATTGTAATGT+TGG + Chr8:54479676-54479695 None:intergenic 20.0%
!! TGCCCAAATTTATTAAAATT+TGG - Chr8:54480222-54480241 MsG0880045119.01.T01:intron 20.0%
!!! AAATTTTAATAAATTTGGGC+AGG + Chr8:54480223-54480242 None:intergenic 20.0%
!!! AACTTATTTTATTTTAGGAC+AGG + Chr8:54479915-54479934 None:intergenic 20.0%
!!! ACTTATTTTATTTTAGGACA+GGG + Chr8:54479914-54479933 None:intergenic 20.0%
!!! CTACTAAATAGTTTTATCTT+TGG - Chr8:54480392-54480411 MsG0880045119.01.T01:intron 20.0%
!!! CTTTTATGAATATTACTACT+TGG - Chr8:54479996-54480015 MsG0880045119.01.T01:intron 20.0%
!!! GTTACATTTTAATCAAACTT+TGG - Chr8:54479366-54479385 MsG0880045119.01.T01:intron 20.0%
!!! GTTGGTTTTAATTTTATGTT+TGG - Chr8:54479479-54479498 MsG0880045119.01.T01:intron 20.0%
!!! TATTCAGACTTTTCTTAATT+GGG - Chr8:54480736-54480755 MsG0880045119.01.T01:CDS 20.0%
!!! TATTTTATCTTTCTTCAGTT+CGG - Chr8:54479339-54479358 MsG0880045119.01.T01:intron 20.0%
!!! TGGTTTTAATTTTATGTTTG+GGG - Chr8:54479481-54479500 MsG0880045119.01.T01:intron 20.0%
!!! TTATTCAGACTTTTCTTAAT+TGG - Chr8:54480735-54480754 MsG0880045119.01.T01:CDS 20.0%
!!! TTTTAATAAACCATTCTCTA+AGG - Chr8:54479833-54479852 MsG0880045119.01.T01:intron 20.0%
! AAACAAGAAAAGAAACCAAA+AGG + Chr8:54480317-54480336 None:intergenic 25.0%
! AAATTGTAATGTTGGATGAT+AGG + Chr8:54479668-54479687 None:intergenic 25.0%
! AGAAAGCAAATATATTTGGT+TGG - Chr8:54479461-54479480 MsG0880045119.01.T01:intron 25.0%
! CTTATAAATACTTGTATCGT+TGG - Chr8:54480847-54480866 MsG0880045119.01.T01:CDS 25.0%
! GACGTTATAACAATACAATA+TGG + Chr8:54480771-54480790 None:intergenic 25.0%
! GTCTTTATGACAATACAATA+TGG + Chr8:54480828-54480847 None:intergenic 25.0%
! TGTCATGAAGTTTCAAATTA+AGG - Chr8:54478794-54478813 MsG0880045119.01.T01:three_prime_UTR 25.0%
! TTATCATTCTCTAATAGTTC+AGG - Chr8:54479794-54479813 MsG0880045119.01.T01:intron 25.0%
! TTTCATTTCCACAACAAAAA+AGG - Chr8:54478591-54478610 MsG0880045119.01.T01:three_prime_UTR 25.0%
!! ATATTTTGCCATAATAAGCA+TGG - Chr8:54479278-54479297 MsG0880045119.01.T01:intron 25.0%
!! ATTCAGACTTTTCTTAATTG+GGG - Chr8:54480737-54480756 MsG0880045119.01.T01:CDS 25.0%
!! TATTTTTCGCTTGAGAATAT+TGG - Chr8:54479238-54479257 MsG0880045119.01.T01:intron 25.0%
!!! ACAGCTTATTTTTCTACTTA+TGG - Chr8:54479532-54479551 MsG0880045119.01.T01:intron 25.0%
!!! CAAATTAAGGTTGATGATAA+TGG - Chr8:54478807-54478826 MsG0880045119.01.T01:three_prime_UTR 25.0%
!!! CTTATTTTATTTTAGGACAG+GGG + Chr8:54479913-54479932 None:intergenic 25.0%
!!! TAGGTTGTTTTTTCATGTAT+TGG - Chr8:54478896-54478915 MsG0880045119.01.T01:three_prime_UTR 25.0%
!!! TGGTTTGTTATAGGAAATTA+AGG - Chr8:54478916-54478935 MsG0880045119.01.T01:three_prime_UTR 25.0%
!!! TTATTTTATTTTAGGACAGG+GGG + Chr8:54479912-54479931 None:intergenic 25.0%
!!! TTCATGTATTGGTTTGTTAT+AGG - Chr8:54478907-54478926 MsG0880045119.01.T01:three_prime_UTR 25.0%
!!! TTGGTTTCTTTTCTTGTTTT+TGG - Chr8:54480318-54480337 MsG0880045119.01.T01:intron 25.0%
!!! TTTCTTTTCCTTTTTTGTTG+TGG + Chr8:54478602-54478621 None:intergenic 25.0%
!!! TTTCTTTTCTTGTTTTTGGA+AGG - Chr8:54480322-54480341 MsG0880045119.01.T01:intron 25.0%
!!! TTTTAATCAAACTTTGGCTT+TGG - Chr8:54479372-54479391 MsG0880045119.01.T01:intron 25.0%
AACTGTTTCCATGCTTATTA+TGG + Chr8:54479289-54479308 None:intergenic 30.0%
AATAAGCTGTTTATCCAAAC+AGG + Chr8:54479522-54479541 None:intergenic 30.0%
ATAAGCTGTTTATCCAAACA+GGG + Chr8:54479521-54479540 None:intergenic 30.0%
ATGAAAAAACAACCTACCAA+CGG + Chr8:54478892-54478911 None:intergenic 30.0%
ATTAAGAACACGTAAGTAAC+TGG - Chr8:54479089-54479108 MsG0880045119.01.T01:intron 30.0%
TAAGATTAAATCCTGCAGAT+CGG + Chr8:54480372-54480391 None:intergenic 30.0%
TCATAAAAGTTAGTATTCGC+TGG + Chr8:54479985-54480004 None:intergenic 30.0%
TCATTAGTATGATTCTCCAA+TGG - Chr8:54480677-54480696 MsG0880045119.01.T01:CDS 30.0%
! GATGAAGTTTCTTGTTTGAA+TGG - Chr8:54480869-54480888 MsG0880045119.01.T01:CDS 30.0%
! TTTTCGTGCACTCTTTAATT+TGG + Chr8:54479725-54479744 None:intergenic 30.0%
!! AAGCAAATTGTGTTTTTGTC+TGG - Chr8:54479137-54479156 MsG0880045119.01.T01:intron 30.0%
!! TGTTTGAATGGTTTGTTTGT+AGG - Chr8:54480881-54480900 MsG0880045119.01.T01:CDS 30.0%
AACATAGCAGCAAAAGATGA+TGG + Chr8:54480289-54480308 None:intergenic 35.0%
AATAGCTCGACCTATAGAAT+AGG - Chr8:54480098-54480117 MsG0880045119.01.T01:intron 35.0%
ACTACGAAAATAAGGAAGAG+CGG - Chr8:54478730-54478749 MsG0880045119.01.T01:three_prime_UTR 35.0%
ATAGCTCGACCTATAGAATA+GGG - Chr8:54480099-54480118 MsG0880045119.01.T01:intron 35.0%
CGTCTTACAAATACTTGTGT+CGG - Chr8:54480787-54480806 MsG0880045119.01.T01:CDS 35.0%
GAAGTTTGTGTATTACTACG+TGG - Chr8:54479162-54479181 MsG0880045119.01.T01:intron 35.0%
! CATCTTTTGCTGCTATGTTT+TGG - Chr8:54480290-54480309 MsG0880045119.01.T01:intron 35.0%
! GAGTGTTTTTCCTTAGAGAA+TGG + Chr8:54479846-54479865 None:intergenic 35.0%
!! GATTCTTGCTTTAAGACCTT+TGG - Chr8:54478840-54478859 MsG0880045119.01.T01:three_prime_UTR 35.0%
!! TTTGGTAATGCTTCCATGAT+TGG - Chr8:54480410-54480429 MsG0880045119.01.T01:intron 35.0%
!! TTTTGTTGAAGATGGGTTTC+TGG - Chr8:54479420-54479439 MsG0880045119.01.T01:intron 35.0%
!!! CTTTTCTTGTTTTTGGAAGG+AGG - Chr8:54480325-54480344 MsG0880045119.01.T01:intron 35.0%
AATCCACTCATTAGCACCAT+TGG + Chr8:54480696-54480715 None:intergenic 40.0%
AGAACCCTAATTGCTGGAAT+TGG + Chr8:54478678-54478697 None:intergenic 40.0%
CAGACCAAAACTACCAATCA+TGG + Chr8:54480426-54480445 None:intergenic 40.0%
CCATCAAACAATGCTAGCAA+GGG - Chr8:54480047-54480066 MsG0880045119.01.T01:intron 40.0%
CTACGAAAATAAGGAAGAGC+GGG - Chr8:54478731-54478750 MsG0880045119.01.T01:three_prime_UTR 40.0%
GGTGGATCACTACGAAAATA+AGG - Chr8:54478722-54478741 MsG0880045119.01.T01:three_prime_UTR 40.0%
GGTTAAGGGAAAGCAAATTG+AGG - Chr8:54479055-54479074 MsG0880045119.01.T01:CDS 40.0%
TAGCTACTAGGAAGAGTTTC+CGG - Chr8:54480070-54480089 MsG0880045119.01.T01:intron 40.0%
TCCATCAAACAATGCTAGCA+AGG - Chr8:54480046-54480065 MsG0880045119.01.T01:intron 40.0%
TGAGGATGCTATAAAAGCAG+CGG - Chr8:54480545-54480564 MsG0880045119.01.T01:intron 40.0%
! ATCGTTTTGCAGCTACTGAA+TGG - Chr8:54479033-54479052 MsG0880045119.01.T01:CDS 40.0%
! CTGGATTGCGTTATTTCAAC+TGG + Chr8:54479966-54479985 None:intergenic 40.0%
! GACTTTTCTTAATTGGGGCT+AGG - Chr8:54480742-54480761 MsG0880045119.01.T01:CDS 40.0%
! GTTATTTCAACTGGTCGCTA+TGG + Chr8:54479957-54479976 None:intergenic 40.0%
! TAGGTCGAGCTATTTCATAC+CGG + Chr8:54480092-54480111 None:intergenic 40.0%
! TCGTTTTGCAGCTACTGAAT+GGG - Chr8:54479034-54479053 MsG0880045119.01.T01:CDS 40.0%
!! CTGCTATGTTTTGGACCTTT+TGG - Chr8:54480299-54480318 MsG0880045119.01.T01:intron 40.0%
!! GCTTCCATGATTGGTAGTTT+TGG - Chr8:54480419-54480438 MsG0880045119.01.T01:intron 40.0%
!! TACAAATACTTGTGTCGGCT+TGG - Chr8:54480792-54480811 MsG0880045119.01.T01:CDS 40.0%
!! TGCTTTAAGACCTTTGGATG+TGG - Chr8:54478846-54478865 MsG0880045119.01.T01:three_prime_UTR 40.0%
!!! AGCTTTGGATTCCTTTTCCT+TGG + Chr8:54480167-54480186 None:intergenic 40.0%
!!! TTTATTTTAGGACAGGGGGT+GGG + Chr8:54479908-54479927 None:intergenic 40.0%
!!! TTTTATTTTAGGACAGGGGG+TGG + Chr8:54479909-54479928 None:intergenic 40.0%
AAATAAGGAAGAGCGGGATG+TGG - Chr8:54478737-54478756 MsG0880045119.01.T01:three_prime_UTR 45.0%
AACGGACGATGAAGCAATAG+CGG + Chr8:54478874-54478893 None:intergenic 45.0%
AATAAGGAAGAGCGGGATGT+GGG - Chr8:54478738-54478757 MsG0880045119.01.T01:three_prime_UTR 45.0%
AGTCGCCAATTCCAGCAATT+AGG - Chr8:54478670-54478689 MsG0880045119.01.T01:three_prime_UTR 45.0%
ATGATCGGCAACCAAGGAAA+AGG - Chr8:54480153-54480172 MsG0880045119.01.T01:intron 45.0%
ATGCTGCAGTGAAGCTTAAC+TGG + Chr8:54480519-54480538 None:intergenic 45.0%
CAATGGGAGAAATCATGCAG+TGG + Chr8:54480628-54480647 None:intergenic 45.0%
CACTCCTCTCCCTATTCTAT+AGG + Chr8:54480111-54480130 None:intergenic 45.0%
CATTCCAGCATTCCAGCAAT+GGG + Chr8:54480644-54480663 None:intergenic 45.0%
CCCTTGCTAGCATTGTTTGA+TGG + Chr8:54480050-54480069 None:intergenic 45.0%
CGCAAAGAAACTCGTCCAAA+CGG - Chr8:54478638-54478657 MsG0880045119.01.T01:three_prime_UTR 45.0%
CTATAGAATAGGGAGAGGAG+TGG - Chr8:54480109-54480128 MsG0880045119.01.T01:intron 45.0%
CTTCATGACATCACCACAAG+CGG + Chr8:54478784-54478803 None:intergenic 45.0%
GACGTGTAGCTAAGTAGCTT+TGG + Chr8:54480182-54480201 None:intergenic 45.0%
GTCGCCAATTCCAGCAATTA+GGG - Chr8:54478671-54478690 MsG0880045119.01.T01:three_prime_UTR 45.0%
TCATTCCAGCATTCCAGCAA+TGG + Chr8:54480645-54480664 None:intergenic 45.0%
TCGACCTATAGAATAGGGAG+AGG - Chr8:54480104-54480123 MsG0880045119.01.T01:intron 45.0%
TGCATGATTTCTCCCATTGC+TGG - Chr8:54480629-54480648 MsG0880045119.01.T01:intron 45.0%
TTATGAAGCTAAGCGTGCCT+CGG - Chr8:54480575-54480594 MsG0880045119.01.T01:intron 45.0%
TTCATGACATCACCACAAGC+GGG + Chr8:54478783-54478802 None:intergenic 45.0%
TTCTTCATGATCGGCAACCA+AGG - Chr8:54480147-54480166 MsG0880045119.01.T01:intron 45.0%
! AATAGCGGAACCACATCCAA+AGG + Chr8:54478859-54478878 None:intergenic 45.0%
! TCTCCAATGGTGCTAATGAG+TGG - Chr8:54480690-54480709 MsG0880045119.01.T01:CDS 45.0%
!! AACTTTGGCTTTGGTGCTTG+AGG - Chr8:54479381-54479400 MsG0880045119.01.T01:intron 45.0%
!! GCAGCTACTGAATGGGTTAA+GGG - Chr8:54479041-54479060 MsG0880045119.01.T01:CDS 45.0%
!! TGCAGCTACTGAATGGGTTA+AGG - Chr8:54479040-54479059 MsG0880045119.01.T01:CDS 45.0%
CGTGGAAGAACCCTAATTGC+TGG + Chr8:54478684-54478703 None:intergenic 50.0%
CTGCAGTGAAGCTTAACTGG+CGG + Chr8:54480516-54480535 None:intergenic 50.0%
GATGTGGGATCTGCTCTTGT+TGG - Chr8:54478753-54478772 MsG0880045119.01.T01:three_prime_UTR 50.0%
GCTATTGCTTCATCGTCCGT+TGG - Chr8:54478873-54478892 MsG0880045119.01.T01:three_prime_UTR 50.0%
GGCGCGATTCAGAAACCAAA+GGG + Chr8:54480351-54480370 None:intergenic 50.0%
TCAACTGGTCGCTATGGTGA+TGG + Chr8:54479951-54479970 None:intergenic 50.0%
TCCACGATTCTACCACGAGA+CGG - Chr8:54478698-54478717 MsG0880045119.01.T01:three_prime_UTR 50.0%
TGCAGTGAAGCTTAACTGGC+GGG + Chr8:54480515-54480534 None:intergenic 50.0%
TGCTAGCAAGGGTAGCTACT+AGG - Chr8:54480058-54480077 MsG0880045119.01.T01:intron 50.0%
TTCTCCCATTGCTGGAATGC+TGG - Chr8:54480637-54480656 MsG0880045119.01.T01:intron 50.0%
TTGAAGATGCGAGCCCTGTT+TGG - Chr8:54479505-54479524 MsG0880045119.01.T01:intron 50.0%
TTGCTTCATCGTCCGTTGGT+AGG - Chr8:54478877-54478896 MsG0880045119.01.T01:three_prime_UTR 50.0%
!! GCTTTGGTGCTTGAGGTTGA+TGG - Chr8:54479388-54479407 MsG0880045119.01.T01:intron 50.0%
!! GTTTTTGGAAGGAGGCCCTT+TGG - Chr8:54480333-54480352 MsG0880045119.01.T01:intron 50.0%
!! TGGTCGCTATGGTGATGGTT+AGG + Chr8:54479946-54479965 None:intergenic 50.0%
ACCGTCTCGTGGTAGAATCG+TGG + Chr8:54478702-54478721 None:intergenic 55.0%
ACGATTCTACCACGAGACGG+TGG - Chr8:54478701-54478720 MsG0880045119.01.T01:three_prime_UTR 55.0%
AGTGGCAGATTTGTCTCCCG+TGG + Chr8:54480610-54480629 None:intergenic 55.0%
ATTCTACCACGAGACGGTGG+TGG - Chr8:54478704-54478723 MsG0880045119.01.T01:three_prime_UTR 55.0%
CGGCGCGATTCAGAAACCAA+AGG + Chr8:54480352-54480371 None:intergenic 55.0%
! TCACTGCAGCATGCTTGCTG+AGG - Chr8:54480527-54480546 MsG0880045119.01.T01:intron 55.0%
!! ACTTGTGTCGGCTTGGAGCT+AGG - Chr8:54480799-54480818 MsG0880045119.01.T01:CDS 55.0%
!!! ATTTTAGGACAGGGGGTGGG+TGG + Chr8:54479905-54479924 None:intergenic 55.0%
!!! TTTTAGGACAGGGGGTGGGT+GGG + Chr8:54479904-54479923 None:intergenic 55.0%
AGTGATCCACCACCGTCTCG+TGG + Chr8:54478713-54478732 None:intergenic 60.0%
CTTGTTGGTGCACCCGCTTG+TGG - Chr8:54478768-54478787 MsG0880045119.01.T01:three_prime_UTR 60.0%
GCGACTCAAACGACGCCGTT+TGG + Chr8:54478656-54478675 None:intergenic 60.0%
TAAGCGTGCCTCGGCTACTG+CGG - Chr8:54480584-54480603 MsG0880045119.01.T01:intron 60.0%
! TCTGAATCGCGCCGATCTGC+AGG - Chr8:54480358-54480377 MsG0880045119.01.T01:intron 60.0%
ACAGGGGGTGGGTGGGTGAG+TGG + Chr8:54479897-54479916 None:intergenic 70.0%
GGTGGGTGGGTGAGTGGTGG+TGG + Chr8:54479891-54479910 None:intergenic 70.0%
TGCCTCGGCTACTGCGGCCA+CGG - Chr8:54480590-54480609 MsG0880045119.01.T01:intron 70.0%
GCCTCGGCTACTGCGGCCAC+GGG - Chr8:54480591-54480610 MsG0880045119.01.T01:intron 75.0%
GGGGGTGGGTGGGTGAGTGG+TGG + Chr8:54479894-54479913 None:intergenic 75.0%
TCCCGTGGCCGCAGTAGCCG+AGG + Chr8:54480595-54480614 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 54478580 54480991 54478580 ID=MsG0880045119.01;Name=MsG0880045119.01
Chr8 mRNA 54478580 54480991 54478580 ID=MsG0880045119.01.T01;Parent=MsG0880045119.01;Name=MsG0880045119.01.T01;_AED=0.50;_eAED=0.50;_QI=47|1|1|1|1|1|3|356|156
Chr8 exon 54478580 54479083 54478580 ID=MsG0880045119.01.T01:exon:23528;Parent=MsG0880045119.01.T01
Chr8 exon 54480472 54480526 54480472 ID=MsG0880045119.01.T01:exon:23527;Parent=MsG0880045119.01.T01
Chr8 exon 54480677 54480991 54480677 ID=MsG0880045119.01.T01:exon:23526;Parent=MsG0880045119.01.T01
Chr8 five_prime_UTR 54480945 54480991 54480945 ID=MsG0880045119.01.T01:five_prime_utr;Parent=MsG0880045119.01.T01
Chr8 CDS 54480677 54480944 54480677 ID=MsG0880045119.01.T01:cds;Parent=MsG0880045119.01.T01
Chr8 CDS 54480472 54480526 54480472 ID=MsG0880045119.01.T01:cds;Parent=MsG0880045119.01.T01
Chr8 CDS 54478936 54479083 54478936 ID=MsG0880045119.01.T01:cds;Parent=MsG0880045119.01.T01
Chr8 three_prime_UTR 54478580 54478935 54478580 ID=MsG0880045119.01.T01:three_prime_utr;Parent=MsG0880045119.01.T01
Gene Sequence

>MsG0880045119.01.T01

ATGCTGAGAATCGCAAAGAAACTCGTCCAAACGGCGTCGTTTGAGTCGCCAATTCCAGCAATTAGGGTTCTTCCACGATTCTACCACGAGACGGTGGTGGATCACTACGAAAATAAGGAAGAGCGGGATGTGGGATCTGCTCTTGTTGGTGCACCCGCTTGTGGTGATGTCATGAAGTTTCAAATTAAGGTTGATGATAATGGAACAATCGTTGATTCTTGCTTTAAGACCTTTGGATGTGGTTCCGCTATTGCTTCATCGTCCGTTGCTACTGAATGGGTTAAGGGAAAGCAAATTGAGGAAGTTTTAAGCATTAAGAACACTGCGATTGCAAAGCATCTTAAACTCCCGCCAGTTAAGCTTCACTGCAGCATGCTTGCTGAGGATGCTATAAAAGCAGCGGTTAAAGATTATGAAGCTAAGCGTGCCTCGGCTACTGCGGCCACGGGAGACAAATCTGCCACTGCATGA
Protein sequence

>MsG0880045119.01.T01

MLRIAKKLVQTASFESPIPAIRVLPRFYHETVVDHYENKEERDVGSALVGAPACGDVMKFQIKVDDNGTIVDSCFKTFGCGSAIASSSVATEWVKGKQIEEVLSIKNTAIAKHLKLPPVKLHCSMLAEDAIKAAVKDYEAKRASATAATGDKSATA*