Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027369.01.T01 | ABD33316.1 | 75.781 | 128 | 31 | 0 | 50 | 177 | 126 | 253 | 1.56E-58 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027369.01.T01 | Q2HRF7 | 75.781 | 128 | 31 | 0 | 50 | 177 | 126 | 253 | 7.47e-59 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003669.01 | MsG0580027369.01 | 0.832994 | 6.878140e-56 | 7.817011e-53 |
| MsG0480020377.01 | MsG0580027369.01 | 0.809270 | 2.055383e-50 | 1.201415e-47 |
| MsG0580027369.01 | MsG0580028394.01 | 0.803009 | 4.276220e-49 | 2.125266e-46 |
| MsG0580027369.01 | MsG0680033379.01 | 0.806342 | 8.618931e-50 | 4.666566e-47 |
| MsG0580027369.01 | MsG0780036498.01 | 0.833272 | 5.864837e-56 | 6.720558e-53 |
| MsG0580027369.01 | MsG0880042800.01 | 0.814781 | 1.293320e-51 | 8.758588e-49 |
| MsG0580027369.01 | MsG0880047191.01 | 0.800329 | 1.516737e-48 | 7.043058e-46 |
| MsG0280008615.01 | MsG0580027369.01 | 0.805649 | 1.205743e-49 | 6.413684e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027369.01.T01 | MTR_7g068350 | 38.298 | 235 | 108 | 3 | 27 | 261 | 47 | 244 | 6.51e-41 | 141 |
| MsG0580027369.01.T01 | MTR_5g098775 | 34.737 | 190 | 64 | 4 | 35 | 224 | 68 | 197 | 3.09e-23 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 70 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGTGGGCGACTCAGATC+TGG | 0.255149 | 5:-59222391 | None:intergenic |
| ATGTTGATGCAATGGTTATT+TGG | 0.272270 | 5:+59222805 | MsG0580027369.01.T01:CDS |
| ACGCGCATTTCGACTATAAT+TGG | 0.298687 | 5:-59222695 | None:intergenic |
| GTGGAAGGGTCATGGTGGTA+TGG | 0.312347 | 5:-59222229 | None:intergenic |
| GATAAGACACGATTGATAAA+AGG | 0.342103 | 5:-59222644 | None:intergenic |
| ATTTACTCTTTAAAACTGTT+GGG | 0.366710 | 5:+59222913 | MsG0580027369.01.T01:CDS |
| CAAATCAACCATGGAGGCTT+TGG | 0.374059 | 5:-59222412 | None:intergenic |
| TATTTACTCTTTAAAACTGT+TGG | 0.388133 | 5:+59222912 | MsG0580027369.01.T01:CDS |
| ACGTGTGGTGTGTTGTGTTT+TGG | 0.392807 | 5:+59222506 | MsG0580027369.01.T01:CDS |
| TGGAAGGGTCATGGTGGTAT+GGG | 0.404833 | 5:-59222228 | None:intergenic |
| AGGGCTGATGTTAACTTTGA+AGG | 0.407861 | 5:-59222943 | None:intergenic |
| TTTATCAATCGTGTCTTATC+CGG | 0.411474 | 5:+59222647 | MsG0580027369.01.T01:CDS |
| CTGGTCACTGCGGCCGCCAC+CGG | 0.414025 | 5:+59222318 | MsG0580027369.01.T01:CDS |
| TTGATGTTTGGAATCACATA+AGG | 0.428439 | 5:+59222578 | MsG0580027369.01.T01:CDS |
| ACCAGAGAAGAGCAGCTGTC+GGG | 0.432023 | 5:-59222300 | None:intergenic |
| AACCATGGAGGCTTTGGTGG+TGG | 0.435706 | 5:-59222406 | None:intergenic |
| TGATGTTGGTGGAAGGGTCA+TGG | 0.441193 | 5:-59222237 | None:intergenic |
| TCTTTCTTTCTATCCAGTTA+CGG | 0.450104 | 5:-59222738 | None:intergenic |
| CCCCGACAGCTGCTCTTCTC+TGG | 0.461416 | 5:+59222299 | MsG0580027369.01.T01:CDS |
| GGTTATTTGGATATGATAGC+TGG | 0.464703 | 5:+59222818 | MsG0580027369.01.T01:CDS |
| CATGGTGGAGGCTCCGGTGG+CGG | 0.466078 | 5:-59222331 | None:intergenic |
| GGGCTGATGTTAACTTTGAA+GGG | 0.467165 | 5:-59222942 | None:intergenic |
| GGGCGACTCAGATCTGGGTC+AGG | 0.469746 | 5:-59222385 | None:intergenic |
| CACAATAGCCTTCACGGTAG+TGG | 0.476719 | 5:-59222359 | None:intergenic |
| GATGAACATGGTGGAGGCTC+CGG | 0.479348 | 5:-59222337 | None:intergenic |
| ATCAACCATGGAGGCTTTGG+TGG | 0.484341 | 5:-59222409 | None:intergenic |
| AAATTTAGCAGAGAAATTTG+AGG | 0.512580 | 5:-59222848 | None:intergenic |
| GAACATGGTGGAGGCTCCGG+TGG | 0.517780 | 5:-59222334 | None:intergenic |
| CTGAATTTGTGCTGCACGTG+TGG | 0.528812 | 5:+59222491 | MsG0580027369.01.T01:CDS |
| TTTACTCTTTAAAACTGTTG+GGG | 0.543205 | 5:+59222914 | MsG0580027369.01.T01:CDS |
| AAGGCAAATAGAATGAAGTC+AGG | 0.543407 | 5:-59222625 | None:intergenic |
| GTGGTGGGCGACTCAGATCT+GGG | 0.544225 | 5:-59222390 | None:intergenic |
| ACCATGGAGGCTTTGGTGGT+GGG | 0.546567 | 5:-59222405 | None:intergenic |
| GATCTGAACGATGGAGGTCA+TGG | 0.548058 | 5:-59222261 | None:intergenic |
| TCATGGATGATGTTGGTGGA+AGG | 0.553564 | 5:-59222244 | None:intergenic |
| GTGTCTTATCCGGTGCGACA+TGG | 0.559102 | 5:+59222657 | MsG0580027369.01.T01:CDS |
| GACCAGAGAAGAGCAGCTGT+CGG | 0.561341 | 5:-59222301 | None:intergenic |
| GCCCACCACCAAAGCCTCCA+TGG | 0.567944 | 5:+59222404 | MsG0580027369.01.T01:CDS |
| AGGTGGCACAATAGCCTTCA+CGG | 0.570394 | 5:-59222365 | None:intergenic |
| GAGGTCATGGATGATGTTGG+TGG | 0.576665 | 5:-59222248 | None:intergenic |
| AAGTCAGGAACAGCCTCATA+CGG | 0.579274 | 5:-59222610 | None:intergenic |
| CCAGAGAAGAGCAGCTGTCG+GGG | 0.581872 | 5:-59222299 | None:intergenic |
| AAAACACAGAGCATTACAAA+GGG | 0.584519 | 5:-59222962 | None:intergenic |
| AGGGTCATGGTGGTATGGGA+TGG | 0.586983 | 5:-59222224 | None:intergenic |
| CCTTTGTCAATATCCGCGGA+TGG | 0.590703 | 5:+59222779 | MsG0580027369.01.T01:CDS |
| CCTCCATCGTTCAGATCTGA+CGG | 0.595004 | 5:+59222267 | MsG0580027369.01.T01:CDS |
| CGACTCAGATCTGGGTCAGG+TGG | 0.597671 | 5:-59222382 | None:intergenic |
| GGCTGATGTTAACTTTGAAG+GGG | 0.604055 | 5:-59222941 | None:intergenic |
| CATGGATGATGTTGGTGGAA+GGG | 0.604915 | 5:-59222243 | None:intergenic |
| TAAAACACAGAGCATTACAA+AGG | 0.605093 | 5:-59222963 | None:intergenic |
| TCTTGTTCACCATGTCGCAC+CGG | 0.608480 | 5:-59222666 | None:intergenic |
| ATGTTCATCCACTACCGTGA+AGG | 0.621869 | 5:+59222351 | MsG0580027369.01.T01:CDS |
| ATTTCCTTTGTCAATATCCG+CGG | 0.634287 | 5:+59222775 | MsG0580027369.01.T01:CDS |
| AAGGTGTGCAGTGCCGTATG+AGG | 0.636801 | 5:+59222597 | MsG0580027369.01.T01:CDS |
| GTAGTGGATGAACATGGTGG+AGG | 0.638473 | 5:-59222343 | None:intergenic |
| CTGGATAGAAAGAAAGACTG+TGG | 0.640096 | 5:+59222744 | MsG0580027369.01.T01:CDS |
| TGTTGGTGGAAGGGTCATGG+TGG | 0.644871 | 5:-59222234 | None:intergenic |
| ATTACACAACAAATCAACCA+TGG | 0.647535 | 5:-59222421 | None:intergenic |
| GATGGACAATGTTGATGCAA+TGG | 0.651297 | 5:+59222797 | MsG0580027369.01.T01:CDS |
| ATGGAGGTCATGGATGATGT+TGG | 0.652536 | 5:-59222251 | None:intergenic |
| GCATCAACATTGTCCATCCG+CGG | 0.656989 | 5:-59222792 | None:intergenic |
| CCATCCGCGGATATTGACAA+AGG | 0.658885 | 5:-59222779 | None:intergenic |
| CCGTCAGATCTGAACGATGG+AGG | 0.659609 | 5:-59222267 | None:intergenic |
| TTCACGGTAGTGGATGAACA+TGG | 0.667373 | 5:-59222349 | None:intergenic |
| CCACCGTCAGATCTGAACGA+TGG | 0.684520 | 5:-59222270 | None:intergenic |
| ACGGTAGTGGATGAACATGG+TGG | 0.723355 | 5:-59222346 | None:intergenic |
| TATGGGATGGCATATGACAG+AGG | 0.727831 | 5:-59222211 | None:intergenic |
| ACACAACAAATCAACCATGG+AGG | 0.736793 | 5:-59222418 | None:intergenic |
| CTGCTCTTCTCTGGTCACTG+CGG | 0.737192 | 5:+59222308 | MsG0580027369.01.T01:CDS |
| CCATCGTTCAGATCTGACGG+TGG | 0.752976 | 5:+59222270 | MsG0580027369.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTACTCTTTAAAACTGTT+GGG | + | Chr5:59222913-59222932 | MsG0580027369.01.T01:CDS | 20.0% |
| !!! | TATTTACTCTTTAAAACTGT+TGG | + | Chr5:59222912-59222931 | MsG0580027369.01.T01:CDS | 20.0% |
| ! | AAATTTAGCAGAGAAATTTG+AGG | - | Chr5:59222851-59222870 | None:intergenic | 25.0% |
| !!! | TTTACTCTTTAAAACTGTTG+GGG | + | Chr5:59222914-59222933 | MsG0580027369.01.T01:CDS | 25.0% |
| ATGTTGATGCAATGGTTATT+TGG | + | Chr5:59222805-59222824 | MsG0580027369.01.T01:CDS | 30.0% | |
| ATTACACAACAAATCAACCA+TGG | - | Chr5:59222424-59222443 | None:intergenic | 30.0% | |
| GATAAGACACGATTGATAAA+AGG | - | Chr5:59222647-59222666 | None:intergenic | 30.0% | |
| TCTTTCTTTCTATCCAGTTA+CGG | - | Chr5:59222741-59222760 | None:intergenic | 30.0% | |
| TTTATCAATCGTGTCTTATC+CGG | + | Chr5:59222647-59222666 | MsG0580027369.01.T01:CDS | 30.0% | |
| ! | AAAACACAGAGCATTACAAA+GGG | - | Chr5:59222965-59222984 | None:intergenic | 30.0% |
| ! | TAAAACACAGAGCATTACAA+AGG | - | Chr5:59222966-59222985 | None:intergenic | 30.0% |
| ! | TTGATGTTTGGAATCACATA+AGG | + | Chr5:59222578-59222597 | MsG0580027369.01.T01:CDS | 30.0% |
| ! | TTGTAATGCTCTGTGTTTTA+TGG | + | Chr5:59222966-59222985 | MsG0580027369.01.T01:CDS | 30.0% |
| AAGGCAAATAGAATGAAGTC+AGG | - | Chr5:59222628-59222647 | None:intergenic | 35.0% | |
| ATTTCCTTTGTCAATATCCG+CGG | + | Chr5:59222775-59222794 | MsG0580027369.01.T01:CDS | 35.0% | |
| CCAAAAAAATGATCCGTAAC+TGG | + | Chr5:59222725-59222744 | MsG0580027369.01.T01:CDS | 35.0% | |
| GGTTATTTGGATATGATAGC+TGG | + | Chr5:59222818-59222837 | MsG0580027369.01.T01:CDS | 35.0% | |
| !!! | CCAGTTACGGATCATTTTTT+TGG | - | Chr5:59222728-59222747 | None:intergenic | 35.0% |
| ACACAACAAATCAACCATGG+AGG | - | Chr5:59222421-59222440 | None:intergenic | 40.0% | |
| ACGCGCATTTCGACTATAAT+TGG | - | Chr5:59222698-59222717 | None:intergenic | 40.0% | |
| AGGGCTGATGTTAACTTTGA+AGG | - | Chr5:59222946-59222965 | None:intergenic | 40.0% | |
| CTGGATAGAAAGAAAGACTG+TGG | + | Chr5:59222744-59222763 | MsG0580027369.01.T01:CDS | 40.0% | |
| GATGGACAATGTTGATGCAA+TGG | + | Chr5:59222797-59222816 | MsG0580027369.01.T01:CDS | 40.0% | |
| GGGCTGATGTTAACTTTGAA+GGG | - | Chr5:59222945-59222964 | None:intergenic | 40.0% | |
| ! | GGCTGATGTTAACTTTGAAG+GGG | - | Chr5:59222944-59222963 | None:intergenic | 40.0% |
| !! | GTCAGCTGAGTTTTGATGTT+TGG | + | Chr5:59222566-59222585 | MsG0580027369.01.T01:CDS | 40.0% |
| AAGTCAGGAACAGCCTCATA+CGG | - | Chr5:59222613-59222632 | None:intergenic | 45.0% | |
| ATGGAGGTCATGGATGATGT+TGG | - | Chr5:59222254-59222273 | None:intergenic | 45.0% | |
| ATGTTCATCCACTACCGTGA+AGG | + | Chr5:59222351-59222370 | MsG0580027369.01.T01:CDS | 45.0% | |
| CAAATCAACCATGGAGGCTT+TGG | - | Chr5:59222415-59222434 | None:intergenic | 45.0% | |
| TATGGGATGGCATATGACAG+AGG | - | Chr5:59222214-59222233 | None:intergenic | 45.0% | |
| ! | ACGTGTGGTGTGTTGTGTTT+TGG | + | Chr5:59222506-59222525 | MsG0580027369.01.T01:CDS | 45.0% |
| ! | CATGGATGATGTTGGTGGAA+GGG | - | Chr5:59222246-59222265 | None:intergenic | 45.0% |
| ! | TCATGGATGATGTTGGTGGA+AGG | - | Chr5:59222247-59222266 | None:intergenic | 45.0% |
| !! | TTCACGGTAGTGGATGAACA+TGG | - | Chr5:59222352-59222371 | None:intergenic | 45.0% |
| AGGTGGCACAATAGCCTTCA+CGG | - | Chr5:59222368-59222387 | None:intergenic | 50.0% | |
| CACAATAGCCTTCACGGTAG+TGG | - | Chr5:59222362-59222381 | None:intergenic | 50.0% | |
| CCATCCGCGGATATTGACAA+AGG | - | Chr5:59222782-59222801 | None:intergenic | 50.0% | |
| CCTCCATCGTTCAGATCTGA+CGG | + | Chr5:59222267-59222286 | MsG0580027369.01.T01:CDS | 50.0% | |
| CCTTTGTCAATATCCGCGGA+TGG | + | Chr5:59222779-59222798 | MsG0580027369.01.T01:CDS | 50.0% | |
| CTGAATTTGTGCTGCACGTG+TGG | + | Chr5:59222491-59222510 | MsG0580027369.01.T01:CDS | 50.0% | |
| GAGGTCATGGATGATGTTGG+TGG | - | Chr5:59222251-59222270 | None:intergenic | 50.0% | |
| GATCTGAACGATGGAGGTCA+TGG | - | Chr5:59222264-59222283 | None:intergenic | 50.0% | |
| GCATCAACATTGTCCATCCG+CGG | - | Chr5:59222795-59222814 | None:intergenic | 50.0% | |
| TCTTGTTCACCATGTCGCAC+CGG | - | Chr5:59222669-59222688 | None:intergenic | 50.0% | |
| TGGAAGGGTCATGGTGGTAT+GGG | - | Chr5:59222231-59222250 | None:intergenic | 50.0% | |
| ! | ATCAACCATGGAGGCTTTGG+TGG | - | Chr5:59222412-59222431 | None:intergenic | 50.0% |
| ! | TGATGTTGGTGGAAGGGTCA+TGG | - | Chr5:59222240-59222259 | None:intergenic | 50.0% |
| !! | ACGGTAGTGGATGAACATGG+TGG | - | Chr5:59222349-59222368 | None:intergenic | 50.0% |
| !! | GTAGTGGATGAACATGGTGG+AGG | - | Chr5:59222346-59222365 | None:intergenic | 50.0% |
| AAGGTGTGCAGTGCCGTATG+AGG | + | Chr5:59222597-59222616 | MsG0580027369.01.T01:CDS | 55.0% | |
| ACCAGAGAAGAGCAGCTGTC+GGG | - | Chr5:59222303-59222322 | None:intergenic | 55.0% | |
| AGGGTCATGGTGGTATGGGA+TGG | - | Chr5:59222227-59222246 | None:intergenic | 55.0% | |
| CCACCGTCAGATCTGAACGA+TGG | - | Chr5:59222273-59222292 | None:intergenic | 55.0% | |
| CCATCGTTCAGATCTGACGG+TGG | + | Chr5:59222270-59222289 | MsG0580027369.01.T01:CDS | 55.0% | |
| CCGTCAGATCTGAACGATGG+AGG | - | Chr5:59222270-59222289 | None:intergenic | 55.0% | |
| CTGCTCTTCTCTGGTCACTG+CGG | + | Chr5:59222308-59222327 | MsG0580027369.01.T01:CDS | 55.0% | |
| GACCAGAGAAGAGCAGCTGT+CGG | - | Chr5:59222304-59222323 | None:intergenic | 55.0% | |
| GTGGAAGGGTCATGGTGGTA+TGG | - | Chr5:59222232-59222251 | None:intergenic | 55.0% | |
| GTGTCTTATCCGGTGCGACA+TGG | + | Chr5:59222657-59222676 | MsG0580027369.01.T01:CDS | 55.0% | |
| ! | AACCATGGAGGCTTTGGTGG+TGG | - | Chr5:59222409-59222428 | None:intergenic | 55.0% |
| ! | ACCATGGAGGCTTTGGTGGT+GGG | - | Chr5:59222408-59222427 | None:intergenic | 55.0% |
| ! | GATGAACATGGTGGAGGCTC+CGG | - | Chr5:59222340-59222359 | None:intergenic | 55.0% |
| ! | TGTTGGTGGAAGGGTCATGG+TGG | - | Chr5:59222237-59222256 | None:intergenic | 55.0% |
| CCAGAGAAGAGCAGCTGTCG+GGG | - | Chr5:59222302-59222321 | None:intergenic | 60.0% | |
| CGACTCAGATCTGGGTCAGG+TGG | - | Chr5:59222385-59222404 | None:intergenic | 60.0% | |
| !! | GTGGTGGGCGACTCAGATCT+GGG | - | Chr5:59222393-59222412 | None:intergenic | 60.0% |
| CCCCGACAGCTGCTCTTCTC+TGG | + | Chr5:59222299-59222318 | MsG0580027369.01.T01:CDS | 65.0% | |
| GCCCACCACCAAAGCCTCCA+TGG | + | Chr5:59222404-59222423 | MsG0580027369.01.T01:CDS | 65.0% | |
| GGGCGACTCAGATCTGGGTC+AGG | - | Chr5:59222388-59222407 | None:intergenic | 65.0% | |
| ! | GAACATGGTGGAGGCTCCGG+TGG | - | Chr5:59222337-59222356 | None:intergenic | 65.0% |
| !! | GGTGGTGGGCGACTCAGATC+TGG | - | Chr5:59222394-59222413 | None:intergenic | 65.0% |
| ! | CATGGTGGAGGCTCCGGTGG+CGG | - | Chr5:59222334-59222353 | None:intergenic | 70.0% |
| CTGGTCACTGCGGCCGCCAC+CGG | + | Chr5:59222318-59222337 | MsG0580027369.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 59222208 | 59222993 | 59222208 | ID=MsG0580027369.01;Name=MsG0580027369.01 |
| Chr5 | mRNA | 59222208 | 59222993 | 59222208 | ID=MsG0580027369.01.T01;Parent=MsG0580027369.01;Name=MsG0580027369.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|261 |
| Chr5 | exon | 59222208 | 59222993 | 59222208 | ID=MsG0580027369.01.T01:exon:13321;Parent=MsG0580027369.01.T01 |
| Chr5 | CDS | 59222208 | 59222993 | 59222208 | ID=MsG0580027369.01.T01:cds;Parent=MsG0580027369.01.T01 |
| Gene Sequence |
| Protein sequence |
