AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008615.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008615.01.T01 MTR_2g046150 98.734 158 2 0 1 158 7 164 3.70e-113 318
MsG0280008615.01.T01 MTR_7g111900 46.809 141 74 1 1 140 3 143 9.12e-38 127
MsG0280008615.01.T01 MTR_1g084200 43.421 152 85 1 1 151 3 154 1.64e-36 124
MsG0280008615.01.T01 MTR_7g103200 43.704 135 75 1 1 134 3 137 3.38e-36 122
MsG0280008615.01.T01 MTR_2g019480 38.129 139 86 0 9 147 16 154 4.78e-32 112
MsG0280008615.01.T01 MTR_2g091215 40.909 132 78 0 5 136 3 134 9.87e-28 100
MsG0280008615.01.T01 MTR_2g091210 38.462 130 80 0 5 134 3 132 7.56e-27 98.6
MsG0280008615.01.T01 MTR_4g122930 36.986 146 85 4 1 141 3 146 6.42e-24 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008615.01.T01 AT4G24130 69.930 143 43 0 1 143 8 150 2.39e-71 212
MsG0280008615.01.T01 AT1G09310 46.207 145 76 2 1 144 3 146 1.52e-34 119
MsG0280008615.01.T01 AT1G56580 44.595 148 79 3 1 145 3 150 3.10e-34 118
MsG0280008615.01.T01 AT5G46230 37.121 132 83 0 3 134 8 139 1.29e-31 110
MsG0280008615.01.T01 AT1G30020 33.846 130 82 1 1 130 6 131 1.09e-25 96.3
MsG0280008615.01.T01 AT5G49600 34.694 147 85 4 1 137 1 146 1.24e-20 83.6

Find 30 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGAGTTCTTCCCTTAA+AGG 0.135944 2:-35040873 MsG0280008615.01.T01:CDS
ACATTCAACAAGATCCTTTA+AGG 0.179628 2:+35040859 None:intergenic
AGAGTAAGCAATTGTTTGTT+TGG 0.287883 2:-35040688 MsG0280008615.01.T01:CDS
TGTGGAAGAGTAAGAGAAAC+TGG 0.354470 2:-35040839 MsG0280008615.01.T01:CDS
CTTTAAGGGAAGAACTCCTT+TGG 0.355774 2:+35040874 None:intergenic
TCAATACTCATTTCAACTAT+AGG 0.386964 2:+35040662 None:intergenic
ATTTATGAGTGTTGAGGAAA+AGG 0.388563 2:-35040570 MsG0280008615.01.T01:CDS
GAGTAAGCAATTGTTTGTTT+GGG 0.413681 2:-35040687 MsG0280008615.01.T01:CDS
CAACCATTCCATACAACTCT+TGG 0.413753 2:-35040912 MsG0280008615.01.T01:CDS
TTTAAGGGAAGAACTCCTTT+GGG 0.420742 2:+35040875 None:intergenic
GAAGTTGCAATTGAAGGATA+AGG 0.421440 2:-35040537 MsG0280008615.01.T01:CDS
CATTCAACAAGATCCTTTAA+GGG 0.423103 2:+35040860 None:intergenic
ATACAACTCTTGGAAGAGCT+TGG 0.448905 2:-35040902 MsG0280008615.01.T01:CDS
TTTAAGACTCCTATGGGGAT+TGG 0.453850 2:-35040614 MsG0280008615.01.T01:CDS
GTATGAGAAGTTGCAATTGA+AGG 0.453990 2:-35040543 MsG0280008615.01.T01:CDS
ATAGTTGAAATGAGTATTGA+GGG 0.460794 2:-35040659 MsG0280008615.01.T01:CDS
CCTTCAAAATAATGTTCACT+TGG 0.482969 2:+35040791 None:intergenic
AAGCTCTTCCAAGAGTTGTA+TGG 0.483868 2:+35040904 None:intergenic
TATAGTTGAAATGAGTATTG+AGG 0.501110 2:-35040660 MsG0280008615.01.T01:CDS
AATTGAAGTTACTGCTTATG+TGG 0.504724 2:-35040744 MsG0280008615.01.T01:CDS
ACAAAGACTCTTGTGAGCTA+TGG 0.512268 2:-35040767 MsG0280008615.01.T01:CDS
TTAAAGGATCTTGTTGAATG+TGG 0.521582 2:-35040857 MsG0280008615.01.T01:CDS
CTTCCAAGAGTTGTATGGAA+TGG 0.546372 2:+35040909 None:intergenic
GTTGCAATTGAAGGATAAGG+AGG 0.546376 2:-35040534 MsG0280008615.01.T01:CDS
GCAGGTGCAGAAATAGTGTA+TGG 0.551940 2:-35040950 MsG0280008615.01.T01:CDS
TACTTCATTTATGAGTGTTG+AGG 0.556318 2:-35040576 MsG0280008615.01.T01:CDS
TTCAAGATGAAGAAAATGAG+TGG 0.567086 2:-35040716 MsG0280008615.01.T01:CDS
GAAGAGCTTGGATTTCCCAA+AGG 0.599321 2:-35040890 MsG0280008615.01.T01:CDS
ATGGTAACAAATGAACGTGC+AGG 0.612544 2:-35040968 MsG0280008615.01.T01:CDS
AAAGACTTACCAATCCCCAT+AGG 0.653062 2:+35040605 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAATACTCATTTCAACTAT+AGG + Chr2:35040823-35040842 None:intergenic 25.0%
!! TATAGTTGAAATGAGTATTG+AGG - Chr2:35040822-35040841 MsG0280008615.01.T01:CDS 25.0%
!!! ATAGTTGAAATGAGTATTGA+GGG - Chr2:35040823-35040842 MsG0280008615.01.T01:CDS 25.0%
!!! ATGTATTTTAAGACTCCTAT+GGG - Chr2:35040862-35040881 MsG0280008615.01.T01:CDS 25.0%
!!! TTTTGTTTGGATGAAACAAA+AGG - Chr2:35040666-35040685 MsG0280008615.01.T01:CDS 25.0%
AATTGAAGTTACTGCTTATG+TGG - Chr2:35040738-35040757 MsG0280008615.01.T01:CDS 30.0%
ACATTCAACAAGATCCTTTA+AGG + Chr2:35040626-35040645 None:intergenic 30.0%
AGAGTAAGCAATTGTTTGTT+TGG - Chr2:35040794-35040813 MsG0280008615.01.T01:CDS 30.0%
CATTCAACAAGATCCTTTAA+GGG + Chr2:35040625-35040644 None:intergenic 30.0%
CCTTCAAAATAATGTTCACT+TGG + Chr2:35040694-35040713 None:intergenic 30.0%
GAGTAAGCAATTGTTTGTTT+GGG - Chr2:35040795-35040814 MsG0280008615.01.T01:CDS 30.0%
TTAAAGGATCTTGTTGAATG+TGG - Chr2:35040625-35040644 MsG0280008615.01.T01:CDS 30.0%
TTCAAGATGAAGAAAATGAG+TGG - Chr2:35040766-35040785 MsG0280008615.01.T01:CDS 30.0%
! TACTTCATTTATGAGTGTTG+AGG - Chr2:35040906-35040925 MsG0280008615.01.T01:CDS 30.0%
!! ATTTATGAGTGTTGAGGAAA+AGG - Chr2:35040912-35040931 MsG0280008615.01.T01:CDS 30.0%
!!! ATGAGTATTGAGGGTTTTAA+TGG - Chr2:35040832-35040851 MsG0280008615.01.T01:CDS 30.0%
!!! CCAAGTGAACATTATTTTGA+AGG - Chr2:35040691-35040710 MsG0280008615.01.T01:CDS 30.0%
!!! GATGTATTTTAAGACTCCTA+TGG - Chr2:35040861-35040880 MsG0280008615.01.T01:CDS 30.0%
!!! TAAGAGAAACTGGTTTTGTT+TGG - Chr2:35040653-35040672 MsG0280008615.01.T01:CDS 30.0%
!!! TGTATTTTAAGACTCCTATG+GGG - Chr2:35040863-35040882 MsG0280008615.01.T01:CDS 30.0%
GAAGTTGCAATTGAAGGATA+AGG - Chr2:35040945-35040964 MsG0280008615.01.T01:CDS 35.0%
GTATGAGAAGTTGCAATTGA+AGG - Chr2:35040939-35040958 MsG0280008615.01.T01:CDS 35.0%
TTTAAGGGAAGAACTCCTTT+GGG + Chr2:35040610-35040629 None:intergenic 35.0%
AAAGACTTACCAATCCCCAT+AGG + Chr2:35040880-35040899 None:intergenic 40.0%
AAGCTCTTCCAAGAGTTGTA+TGG + Chr2:35040581-35040600 None:intergenic 40.0%
ACAAAGACTCTTGTGAGCTA+TGG - Chr2:35040715-35040734 MsG0280008615.01.T01:CDS 40.0%
ATACAACTCTTGGAAGAGCT+TGG - Chr2:35040580-35040599 MsG0280008615.01.T01:CDS 40.0%
ATGGTAACAAATGAACGTGC+AGG - Chr2:35040514-35040533 MsG0280008615.01.T01:CDS 40.0%
CAAAGGAGTTCTTCCCTTAA+AGG - Chr2:35040609-35040628 MsG0280008615.01.T01:CDS 40.0%
CAACCATTCCATACAACTCT+TGG - Chr2:35040570-35040589 MsG0280008615.01.T01:CDS 40.0%
CTTCCAAGAGTTGTATGGAA+TGG + Chr2:35040576-35040595 None:intergenic 40.0%
GTTGCAATTGAAGGATAAGG+AGG - Chr2:35040948-35040967 MsG0280008615.01.T01:CDS 40.0%
TGTGGAAGAGTAAGAGAAAC+TGG - Chr2:35040643-35040662 MsG0280008615.01.T01:CDS 40.0%
! CTTTAAGGGAAGAACTCCTT+TGG + Chr2:35040611-35040630 None:intergenic 40.0%
! TTTAAGACTCCTATGGGGAT+TGG - Chr2:35040868-35040887 MsG0280008615.01.T01:CDS 40.0%
GAAGAGCTTGGATTTCCCAA+AGG - Chr2:35040592-35040611 MsG0280008615.01.T01:CDS 45.0%
GCAGGTGCAGAAATAGTGTA+TGG - Chr2:35040532-35040551 MsG0280008615.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr2 gene 35040514 35040990 35040514 ID=MsG0280008615.01;Name=MsG0280008615.01
Chr2 mRNA 35040514 35040990 35040514 ID=MsG0280008615.01.T01;Parent=MsG0280008615.01;Name=MsG0280008615.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|158
Chr2 exon 35040514 35040990 35040514 ID=MsG0280008615.01.T01:exon:20447;Parent=MsG0280008615.01.T01
Chr2 CDS 35040514 35040990 35040514 ID=MsG0280008615.01.T01:cds;Parent=MsG0280008615.01.T01
Gene Sequence

>MsG0280008615.01.T01

ATGGTAACAAATGAACGTGCAGGTGCAGAAATAGTGTATGGTTCTGAAGAATGTTTCAACCATTCCATACAACTCTTGGAAGAGCTTGGATTTCCCAAAGGAGTTCTTCCCTTAAAGGATCTTGTTGAATGTGGAAGAGTAAGAGAAACTGGTTTTGTTTGGATGAAACAAAAGGCACCAAGTGAACATTATTTTGAAGGTACAAAGACTCTTGTGAGCTATGGAATTGAAGTTACTGCTTATGTGGAGAAATTCAAGATGAAGAAAATGAGTGGAATTAAGAGTAAGCAATTGTTTGTTTGGGTGCCTATAGTTGAAATGAGTATTGAGGGTTTTAATGGAAAGAAGATGTATTTTAAGACTCCTATGGGGATTGGTAAGTCTTTTCATGTTACTTCATTTATGAGTGTTGAGGAAAAGGAAAAGTATGAGAAGTTGCAATTGAAGGATAAGGAGGTTGAGATCAAAGAAAATTAA

Protein sequence

>MsG0280008615.01.T01

MVTNERAGAEIVYGSEECFNHSIQLLEELGFPKGVLPLKDLVECGRVRETGFVWMKQKAPSEHYFEGTKTLVSYGIEVTAYVEKFKMKKMSGIKSKQLFVWVPIVEMSIEGFNGKKMYFKTPMGIGKSFHVTSFMSVEEKEKYEKLQLKDKEVEIKEN*